| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 1,270,066 | A→T | 5.6% | T375T (ACA→ACT) | trmFO → | Methylenetetrahydrofolate‑‑tRNA‑(uracil‑5‑)‑ methyltransferase TrmFO |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 1,270,066 | 0 | A | T | 5.6% | 313.0 / 5.8 | 105 | T375T (ACA→ACT) | trmFO | Methylenetetrahydrofolate‑‑tRNA‑(uracil‑5‑)‑ methyltransferase TrmFO |
| Reads supporting (aligned to +/- strand): ref base A (53/43); new base T (4/2); total (58/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.92e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 109 total aligned reads displayed.
TCACAACCAAACATACAGTTTGCGGGACAAATGACTGGTGTTGAAGGTTATGTAGAAAGCGCAGCTAGCGGCTTAGTTGCAGGTATCAATCTTGCGCATAAAATATTAGGCAAGGGTGAGGTAGTATTTCCGAGAGAAACAATGATTGGAAGTATGGCTTACTATATTTCTCATGCTAAAAACAATAAGAATTTCCAACCTATGAATGCTAACTTCGGGTTATTACCATCTTTAGAAACTAGAATTAAAGATAAAAAAGAACGCTATGAAGCACAAGCTAA > USA300TCH1516_ALE/1269926‑1270206 | tCACAACCAAACATACAGTTTGCGGGACAAATGACTGGTGTTGCAGGTTCTGTAGAAAGCGCAGCTAGCGGCTTAGTTGCAGGTATCAATCTTGCGCATAAAATATTAGGCAAGGGTGAGGTAGTATTTCCGAGAGAAACa > 2:604359/1‑141 (MQ=255) acaACCAAACATACAGTTTGCGGGACAAATGACTGGTGTTGAAGGTTATGTAGAAAGCGCAGCTAGCGGCTTAGTTGCAGGTATCAATCTTGCGCATAAAATATTAGGCAAGGGTGAGGTAGTATTTCCGAGAGAAACAAt > 3:331938/1‑141 (MQ=255) caACCAAACATACAGTTTGCGGGACAAATGACTGGTGTTGAAGGTTATGTAGAAAGCGCAGCTAGCGGCTTAGTTGCAGGTATCAATCTTGCGCATAAAATATTAGGCAAGGGTGAGGTAGTATTTCCGAGAGAAACAATg > 1:112998/1‑141 (MQ=255) cAAACATACAGTTTGCGGGACACATGACTGGTGTTGAAGGTTATGTAGAAAGCGCAGCTAGCGGCTTAGTTGCAGGTATCAATCTTGCGCATAAACTATTAGGCAGGGGTGAGGAAGTATTTACGAGAGAAACAATGATTg > 2:1074093/1‑141 (MQ=255) cAAACATACAGTTTGCGGGACAAATGACTGGTGTTGAAGGTTATGTAGAAAGCGCAGCTAGCGGCTTAGTTGCAGGTATCAATCTTGCGCATAAAATATTAGGCAAGGGTGAGGTAGTATTTCCGAGAGAAACAATGATTg < 2:619933/141‑1 (MQ=255) cAAACATACAGTTTGCGGGACAAATGACTGGTGTTGAAGGTTATGTAGAAAGCGCAGCTAGCGGCTTAGTTGCAGGTATCAATCTTGCGCATAAAATATTAGGCAAGGGTGAGGTAGTATTTCCGAGAGAAACAATGATTg < 2:433367/141‑1 (MQ=255) cAAACATACAGTTTGCAGGACAAATGACTGGTGTTGAAGGTTATGTAGAAAGCGCAGCTAGCGGCTTAGTTGCAGGTATCAATCTTGCGCATAAAATATTAGGCAAGGGTGAGGTAGTATTTCCGAGAGAAACAATGATTg < 2:534087/141‑1 (MQ=255) aagcATACAGTTTGCGGGACAAATGACTGGTGTCGAAGGTTATGTAGAAAGCGCAGCTAGCGGCTTAGTTGCAGGTATCAATCTTGCGAATAAAATATTAGGCAAGGGTGACGTAGTATGTCCGAGAGAAACAATGATTgg < 4:345306/138‑1 (MQ=255) aaaCATACAGTTTGCGGGACAAATGACTGGTGTTGAAGGTTATGTAGAAAGCGCAGCTAGCGGCTTAGTTGCAGGTATCAATCTTGCGCATAAAATATTAGGCAAGGGTGAGGTAGTATTTCCGAGAGAAACAATGATTgg < 1:583778/141‑1 (MQ=255) aCATACAGTTTGCGGGACAAATGACTGGTGTTGAAGGTTATGTAGAAAGCGCAGCTAGCGGCTTAGTTGCAGGTATCAATCTTGCGCATAAAATATTAGGCAAGGGTGAGGTAGTATTTCCGAGAGAAACAATGATTGGaa > 4:56121/1‑141 (MQ=255) cATACAGTTTGCGGGACAAATGACTGGTGTTGAAGGTTATGTAGAAAGCGCAGCTAGCGGCTTAGTTGCAGGTATCAATCTTGCGCATAAAATATTAGGCAAGGGTGAGGTAGTATTTCCGAGAGAAACAATGATTGGAAg < 2:727767/141‑1 (MQ=255) gtatgTTTGCGGGACAAATGACTGGTGTTGAAGGTTATGTAGAAAGCGCAGCTAGCGGCTTAGTTGCAGGTATCAATCTTGCGCATAAAATATTAGGCAAGGGTGAGGTAGTATTTCCGAGAGAAACAATGATTGGAAGTa > 1:424191/5‑141 (MQ=255) aCAGTTTGCGGGACAAATGACTGGTGTTGAAGGTTATGTAGAAAGCGCAGCTAGCGGCTTAGTTGCAGGTATCAATCTTGCGCATAAAATATTAGGCAAGGGTGAGGTAGTATTTCCGAGAGAAACAATGATTGGAAGTAt > 3:319383/1‑141 (MQ=255) cAGTTTGCGGGACAAATGACTGGTGTTGAAGGTTATGTAGAAAGCGCAGCTAGCGGCTTAGTTGCAGGTATCAATCTTGCGCATAAAATATTAGGCAAGGGTGAGGTAGTATTTCCGAGAGAAACAATGATTGGAAGTATg > 4:20975/1‑141 (MQ=255) cAGTTTGCGGGACAAATGACTGGTGTTGAAGGTTAAGTAGAAAGCGCAGCTAGCGGCTTAGTTGCAGGTATCAATCTTGCGCATAAACTATTAGGCCAGGGTGAGGTAGTATTTCCGAGGGAACGGAGGATTGGAAGTATg > 3:24166/1‑141 (MQ=255) gCGGGACAAATGACTGGTGTTGAAGGTTATGTAGAAAGCGCAGCTAGCGGCTTAGTTGCAGGTATCAATCTTGCGCATAAAATATTAGGCAAGGGTGAGGTAGTATTTCCGAGAGAAACAATGATTGGAAGTATGGCTTAc < 2:521502/141‑1 (MQ=255) gCGGGACAAATGACTGGTGTTGAAGGTTATGTAGAAAGCGCAGCTAGCGGCTTAGTTGCAGGTATCAATCTTGCGCATAAAATATTAGGCAAGGGTGAGGTAGTATTTCCGAGAGAAACAATGATTGGAAGTATGGCTTAc < 1:685419/141‑1 (MQ=255) gCGGGACAAATGACTGGTGTTGAAGGTTATGTAGAAAGCGCAGCTAGCGGCTTAGTTGCAGGTATCAATCTTGCGCATAAAATATTAGGCAAGGGTGAGGTAGTATTTCCGAGAGAAACAATGATTGGAAGTATGGCTTAc > 1:213358/1‑141 (MQ=255) cGGGACAAATGACTGGTGTTGAAGGTTATGTAGAAAGCGCAGCTAGCGGCTTAGTTGCAGGTATCAATCTTGCGCATAAAATATTAGGCAAGGGTGAGGTAGTATTTCCGAGAGAAACAATGATTGGAAGTATGGCTTACt < 4:325081/141‑1 (MQ=255) ggACAGATGACTGGTGTTGAAGGTTATGTAGAAAGCGCAGCTAGCGGCTTAGTTGCAGGTATCAATCTTGCGCATAAAATATTAGGCAAGGGTGAGGTAGTATTTCCGAGAGAAACAATGATTGGAAGTATGGCTTACtat < 1:503352/141‑1 (MQ=255) ggACAAATGACTGGTGTTGAAGGTTATGTAGAAAGCGCAGCTAGCGGCTTAGTTGCAGGTATCAATCTTGCGCATAAAATATTAGGCAAGGGTGAGGTAGTATTTCCGAGAGAAACAATGATTGGAAGTATGGCTTACtat < 2:68396/141‑1 (MQ=255) gACAAATGACTGGTGTTGAAGGTTATGTAGAAAGCGCAGCTAGCGGCTTAGTTGCAGGTATCAATCTTGCGCATAAAATATTAGGCAAGGGTGAGGTAGTATTTCCGAGAGAAACAATGATTGGAAGTATGGCTTACtata > 1:905180/1‑141 (MQ=255) aCAAATGACTGGTGTTGAAGGTTATGTAGAAAGCGCAGCTAGCGGCTTAGTTGCAGGTATCAATCTTGCGCATAAAATATTAGGCAAGGGTGAGGTAGTATTTCCGAGAGAAACAATGATTGGAAGTATGGCTTACTatat > 2:261865/1‑141 (MQ=255) aaaTGACTGGTGTTGAAGGTTATGTAGAAAGCGCAGCTAGCGGCTTAGTTGCAGGTATCAATCTTGCGCATAAAATATTAGGCAAGGGTGAGGTAGTATTTCCGAGAGAAACAATGATTGGAAGTATGGCTTACTATAttt < 1:789976/141‑1 (MQ=255) aTGACTGGTGTTGAAGGTTATGTAGAAAGCGCAGCTAGCGGCTTAGTTGCAGGTATCAATCTTGCGCATAAAATATTAGGCAAGGGTGAGGTAGTATTTCCGAGAGAAACAATGATTGGAAGTATGGCTTACTATATTtct > 1:45243/1‑141 (MQ=255) gACTGGTGTTGAAGGTTATGTAGAAAGCGCAGCTAGCGGCTTAGTTGCAGGTATCAATCTTGCGCATAAAATATTAGGCAAGGGTGAGGTAGTATTTCCGAGAGAAACAATGATTGGAAGTATGGCTTACTATATTTCTCa < 1:107725/141‑1 (MQ=255) aCTGGTGTTGAAGGTTATGTAGAAAGCGCAGCTAGCGGCTTAGTTGCAGGTATCAATCTTGCGCATGAAATATTAGGCAAGGGGGAGGGAGTATTTCCGTGAGAAACAATGATTGGAAGTATGGCTTACTATATTTCTCAt > 1:376296/1‑141 (MQ=255) aCTGGTGTTGAAGGTTATGTAGAAAGCGCAGCTAGCGGCTTAGTTGCAGGTATCAATCTTGCGCATAAAATATTAGGCAAGGGTGAGGTAGTATTTCCGAGAGAAACAATGATTGGAAGTATGGCTTACTATATTTCTCAt > 3:417122/1‑141 (MQ=255) cTGGTGTTGAAGGTTATGTAGAAAGCGCAGCTAGCGGCTTAGTTGCAGGTATCAATCTTGCGCATAAAATATTAGGCAAGGGTGAGGTAGTATTTCCGAGAGAAACAATGATTGGAAGTATGGCTTACTATATTTCTCATg < 2:29312/141‑1 (MQ=255) tgtTGAAGGTTATGTAGAAAGCGCAGCTAGCGGCTTAGTTGCAGGTATCAATCTTGCGCATAAAATATTAGGCAAAGGTGAGGTAGTATTTCCGAGAGAAACTATGATTGGAAGTATGGCTTACTATATTTCTCATGCTaa > 4:314616/1‑141 (MQ=255) gtTGAAGGTTATGTAGAAAGCGCAGCTAGCGGCTTAGTTGCAGGTATCAATCTTGCGCATAAAATATTAGGCAAGGGTGAGGTAGTATTTCCGAGAGAAACAATGATTGGAAGTATGGCTTACTATATTTCTCATGCTaaa > 1:608229/1‑141 (MQ=255) gAAGGTTATGTAGAAAGCGCAGCTAGCGGCTTAGTTGCAGGTATCAATCTTGCGCATAAAATATTAGGCAAGGGTGAGGTAGTATTTCCGAGAGAAACAATGATTGGAAGTATGGCTTACTATATTTCTCATGCTAAAAAc > 3:205517/1‑141 (MQ=255) aGGTTATGTAGAAAGCGCAGCTAGCGGCTTAGTTGCAGGTATCAATCTTGCGCATAAAATATTAGGCAAGGGTGAGGTAGTATTTCCGAGAGAAACAATGATTGGAAGTATGGCTTACTATATTTCTCATGCTAAAAACaa > 2:380170/1‑141 (MQ=255) tATGTAGAAAGCGCAGCTAGCGGCTTAGTTGCAGGTATCAATCTTGCGCATAAAATATTAGGCAAGGGTGAGGTAGTATTTCCGAGAGAAACAATGATTGGAAGTATGGCTTACTATATTTCTCATGCTAAAAACAATAAg < 2:172744/141‑1 (MQ=255) aTGTAGAAAGCGCAGCTAGCGGCTTAGTTGCAGGTATCAATCTTGCGCATAAAATTTTAGGCAAAGGTGAGGTAGTATTTCCGAGAGAAACTATGATTGGAAGTATGGCTTACTATATTTCTCATGCTAAAAACAATAAGa < 3:247032/141‑1 (MQ=255) gTAGAAAGCGCAGCTAGCGGCTTAGTTGCAGGTATCAATCTTGCGCATAAAATATTAGGCAAGGGTGAGGTAGTATTTCCGAGAGAAACAATGATTGGAAGTATGGCTTACTATATTTCTCATGCTAAAAACAATAAGAAt > 2:957876/1‑141 (MQ=255) gTAGAAAGCGCAGCTAGCGGCTTAGTTGCAGGTATCAATCTTGCGCATAAAATATTAGGCAAGGGTGAGGTAGTATTTCCGAGAGAAACAATGATTGGAAGTATGGCTTACTATATTTCTCATGCTAAAAACAATAAGAAt > 1:861955/1‑141 (MQ=255) tAGAAAGCGCAGCTAGCGGCTTAGTTGCAGGTATCAATCTTGCGCATAAAATATTAGGCAAGGGTGAGGTAGTATTTCCGAGAGAAACAATGATTGGAAGTATGGCTTACTATATTTCTCATGCTAAAAACAATAAGAAtt < 2:982097/141‑1 (MQ=255) gAAAGCGCAGCTAGCGGCTTAGTTGCAGGTATCAATCTTGCGCATAAAATATTAGGCAAGGGTGAGGTAGTATTTCCGAGAGAAACAATGATTGGAAGTATGGCTTACTATATTTCTCATGCTAAAAACAATAAGAATTTc > 2:55318/1‑141 (MQ=255) gaGCTAGCGGCTTAGTTGCAGGTAGCAATCTTGCGCATAAAATATTAGGCAAGGGTGAGGTAGTATTTCCGAGAGAAACAATGATTGGAAGTATGGCTTACTATATTTCTCATGCTAAAAACAATAAGAATTTCCAACCTa < 4:41212/140‑1 (MQ=255) cAGCTAGCGGCTTAGTTGCAGGTATCAATCTTGCGCATAAAATATTAGGCAAGGGTGAGGTAGTATTTCCGAGAGAAACAATGATTGGAAGTATGGCTTACTATATTTCTCATGCTAAAAACAATAAGAATTTCCAACCTa > 1:310626/1‑141 (MQ=255) cAGCTAGCGGCTTAGTTGCAGGTATCAATCTTGCGCATAAAATATTAGGCAAGGGTGAGGTAGTATTTCCGAGAGAAACAATGATTGGAAGTATGGCTTACTATATTTCTCATGCTAAAAACAATAAGAATTTCCAACCTa > 2:735403/1‑141 (MQ=255) aGCTAGCGGCTTAGTCGCAGGTATCAATCTTGCGCATAAAATATTAGGCAAGGGTGAGGTAGTATTTCCGAGAGAAACAATGATTGGAAGTATGGCTTACTATATTTCTCATGCTAAAAACAATAAGAATTTCCAACCTAt < 1:488775/141‑1 (MQ=255) gCTAGCGGCTTAGTTGCAGGTATCAATCTTGCGCATAAAATATTAGGCAAGGGTGAGGTAGTATTTCCGAGAGAAACAATGATTGGAAGTATGGCTTACTATATTTCTCATGCTAAAAACAATAAGAATTTCCAACCTATg > 1:899329/1‑141 (MQ=255) gCTAGCGGCTTAGTTGCAGGTATCAATCTTGCGCATAAAATATTAGGCAAGGGTGAGGTAGTATTTCCGAGAGAAACAATGATTGGAAGTATGGCTTACTATATTTCTCATGCTAAAAACAATAAGAATTTCCAACCTATg > 3:413505/1‑141 (MQ=255) ggCTTAGTTGCAGGTATCAATCTTGCGCATAAAATATTAGGCAAGGGTGAGGTAGTATTTCCGAGAGAAACAATGATTGGAAGTATGGCTTACTATATTTCTCATGCTAAAAACAATAAGAATTTCCAACCTATGAATGCt < 2:911323/141‑1 (MQ=255) gCTTAGTTGCAGGTATCAATCTTGCGCATAAAATATTAGGCAAGGGTGAGGTAGTATTTCCGAGAGAAACAATGATTGGAAGTATGGCTTACTATATTTCTCATGCTAAAAACAATAAGAATTTCCAACCTATGAATGCTa < 2:213358/141‑1 (MQ=255) cTTAGTTGCAGGTATCAATCTTGCGCATAAAATATTAGGCAAAGGTGAGGTAGTATTTCCGAGAGAAACTATGATTGGAAGTATGGCTTACTATATTTCTCATGCTAAAAACAATAAGAATTTCCAACCTATGAATGCTaa > 1:537339/1‑141 (MQ=255) tAGTTGCAGGTATCAATCTTGCGCATAAAATATTAGGCAAGGGTGAGGTAGTATTTCCGAGAGAAACAATGATTGGAAGTATGGCTTACTATATTTCTCATGCTAAAAACAATAAGAATTTCCAACCTATGAATGCTAACt > 3:411223/1‑141 (MQ=255) aGTTGCAGGTATCAATCTTGCGCATAAAATATTAGGCAAGGGTGAGGTAGTATTTCCGAGAGAAACAATGATTGGAAGTATGGCTTACTATATTTCTCATGCTAAAAACAATAAGAATTTCCAACCTATGAATGCTAACtt > 2:435913/1‑141 (MQ=255) ttGCAGGTATCAATCTTGCGCATAAAATATTAGGCAAGGGTGAGGTAGTATTTCCGAGAGAAACAATGATTGGAAGTATGGCTTTCTATATTTCTCATGCTAAAAACAATAAGAATTTCCAACCTATGAATGCTAACTTCg > 2:414322/1‑141 (MQ=255) tGCAGGTATCAATCTTGCGCATAAAATATTAGGCAAAGGTGAGGTAGTATTTCCGAGAGAAACTATGATTGGAAGTATGGCTTACTATATTTCTCATGCTAAAAACAATAAGAATTTCCAACCTATGAATGCTAACTTCgg > 1:255814/1‑141 (MQ=255) cAGGTATCAATCTTGCGCATAAAATATTAGGCAAGGGTGAGGTAGTATTTCCGAGAGAAACAATGATTGGAAGTATGGCTTACTATATTTCTCATGCTAAAAACAATAAGAATTTCCAACCTATGAATGCTAACTTCGGGt > 1:30541/1‑141 (MQ=255) aGGTATCAATCTTGCGCATAAAATATTAGGCAAGGGTGAGGTAGTATTTCCGAGAGAAACAATGATTGGAAGTATGGCTTACTATATTTCTCATGCTAAAAACAATAAGAATTTCCAACCTATGAATGCTAACTTCGGGtt > 4:276967/1‑141 (MQ=255) tCAATCTTGCGCATAAAATATTAGGCAAGGGTGAGGTAGTATTTCCGAGAGAAACAATGATTGGAAGTATGGCTTACTATATTTCTCATGCTAAAAACAATAAGAATTTCCAACCTATGAATGCTAACTTCGGGTTATTAc < 3:75626/141‑1 (MQ=255) aaTCTTGCGGTGAAAATATTAGGTATTGGTGGTGTAGTATTTCCGATAGGAACAATGATTGGAAGTAGGGCTTACTATGTTGCTCATGCTAAAAACAATAAGAATTTCCAACCTATGAATGCTAACTTCGGGTTATTACCa < 1:217643/141‑1 (MQ=255) aaTCTTGCGCATAAAATATTAGGCAAGGGTGAGGTAGTATTTCCGAGAGAAACAATGATTGGAAGTATGGCTTACTATATTTCTCATGCTAAAAACAATAAGAATTTCCAACCTATGAATGCTAACTTCGGGTTATTACCa > 1:133409/1‑141 (MQ=255) aTCTTGCGCATAAAATATTAGGCAAGGGTGAGGTAGTATTTCCGAGAGAAACAATGATTGGAAGTATGGCTTACTATATTTCTCATGCTAAAAACAATAAGAATTTCCAACCTATGAATGCTAACTTCGGGTTATTACCAt > 2:953711/1‑141 (MQ=255) ttGCGCATAAAATATTAGGCAAGGGTGAGGTAGTATTTCCGAGAGAAACGATGCTTGGAAGTATGGCTTACTATATTTCTCATGCTAAAAACAATAAGAATTTCCAACCTATGAATGCTAACTTCGGGTTATTACCATCtt < 1:1027657/141‑1 (MQ=255) ttGCGCATAAAATATTAGGCAAGGGTGAGGTAGTATTTCCGAGAGAAACAATGATTGGAAGTATGGCTTACTATATTTCTCATGCTAAAAACAATAAGAATTTCCAACCTATGAATGCTAACTTCGGGTTATTACCATCtt > 4:261490/1‑141 (MQ=255) ttGCGCATAAAATATTAGGCAAGGGTGAGGTAGTATTTCCGAGAGAAACAATGATTGGAAGTATGGCTTACTATATTTCTCATGCTAAAAACAATAAGAATTTCCAACCTATGAATGCTAACTTCGGGTTATTACCATCtt > 2:410477/1‑141 (MQ=255) tGCGCATAAAATATTAGGCAAGGGTGAGGTAGTATTTCCGAGAGAAACAATGATTGGAAGTATGGCTTACTATATTTCTCATGCTAAAAACAATAAGAATTTCCAACCTATGAATGCTAACTTCGGGTTATTACCATCttt > 2:870285/1‑141 (MQ=255) gcgcATAAAATATTAGGCAAGGGTGAGGTAGTATTTCCGAGAGAAACAATGATTGGAAGTATGGCTTACTATATTTCTCATGCTAAAAACAATAAGAATTTCCAACCTATGAATGCTAACTTCGGGTTATTACCATCTTTa > 4:262298/1‑141 (MQ=255) cgcATAAAATATTAGGCAAGGGTGAGGTAGTATTTCCGAGAGAAACAATGATTGGAAGTATGGCTTACTATATTTCTCATGCTAAAAACAATAAGAATTTCCAACCTATGAATGCTAACTTCGGGTTATTACCATCTTTAg > 4:370896/1‑141 (MQ=255) cgcATAAAATATTAGGCAAGGGTGAGGTAGTATTTCCGAGAGAAACAATGATTGGAAGTATGGCTTACTATATTTCTCATGCTAAAAACAATAAGAATTTCCAACCTATGAATGCTAACTTCGGGTTATTACCATCTTTAg > 2:506936/1‑141 (MQ=255) cgcATAAAATATTAGGCAAGGGTGAGGTAGTATTTCCGAGAGAAACAATGATTGGAAGTATGGCTTACTATATTTCTCATGCTAAAAACAATAAGAATTTCCAACCTATGAATGCTAACTTCGGGTTATTACCATCTTTAg < 2:780478/141‑1 (MQ=255) cATAAGATATTAGGGAAGGGTGAGGTTGTATTTATGAGAGAAACGATGATTGGAAGTATGGCTCACTATATTTCTCAGGCTAAAAACAATAAGAATTTCCGACCTATGAATGCTAACTTCGGGTTATTACCATCTTTAGaa < 2:73094/141‑1 (MQ=255) cATAAAATATTAGGCAAGGGTGAGGTAGTATTTCCGAGAGAAACAATGATTGGAAGTATGGCTTACTATATTTCTCATGCTAAAAACAATAAGAATTTCCAACCTATGAATGCTAACTTCGGGTTATTACCATCTTTAGaa < 3:213998/141‑1 (MQ=255) tAAAATATTGGGCAAGGGTGAGGTAGTATTTCCGAGAGAAACAATGATTGGAAGTATGGCTTACTATATTTCTCATGCTAAAAACAATAAGAATTTCCAACCCATGAATGCTAACTTCGGGTTATTACCATCTTTAGAAAc < 2:404686/141‑1 (MQ=255) aaaTATTAGGCAAGGGTGAGGTAGTATTTCCGAGAGAAACAATGATTGGAAGTATGGCTTACTATATTTCTCATGCTAAAAACAATAAGAATTTCCAACCTATGAATGCTAACTTCGGGTTATTACCATCTTTAGAAACTa > 2:314399/1‑141 (MQ=255) aaaTATTAGGCAAGGGTGAGGTAGTATTTCCGAGAGAAACAATGATTGGAAGTATGGCTTACTATATTTCTCATGCTAAAAACAATAAGAATTTCCAACCTATGAATGCTAACTTCGGGTTATTACCATCTTTAGAAACTa > 1:921365/1‑141 (MQ=255) ggCAAGGGTGAGGTAGTATTTCCGAGAGAAACAATGATTGGAAGTATGGCTTACTATATTTCTCATGCTAAAAACAATAAGAATTTCCAACCTATGAATGCTAACTTCGGGTTATTACCATCTTTAGAAACTAGAATTaaa < 4:218665/141‑1 (MQ=255) aaGGGTGAGGTAGTATTTCCGAGAGAAACAATGATTGGAAGTATGGCTTACTATATTTCTCATGCTAAAAACAATAAGAATTTCCAACCTATGAATGCTAACTTCGGGTTATTACCATCTTTAGAAACTAGAATTAAAGAt > 2:840897/1‑141 (MQ=255) aGGGTGAGGTAGTATTTCCGAGAGAAACAATGATTGGAAGTATGGCTTACTATATTTCTCATGCTAAAAACAATAAGAATTTCCAACCTATGAATGCTAACTTCGGGTTATTACCATCTTTAGAAACTAGAATTAAAGATa > 3:296703/1‑141 (MQ=255) gggTGAGGTAGTATTTCCGAGAGAAACAATGATTGGAAGTATGGCTTACTATATTTCTCATGCTAAAAACAATAAGAATTTCCAACCTATGAATGCTAACTTCGGGTTATTACCATCTTTAGAAACTAGAATTAAAGATaa < 1:109124/141‑1 (MQ=255) gggTGAGGTAGTATTTCCGAGAGAAACAATGATTGGAAGTATGGCTTACTATATTTCTCATGCTAAAAACAATAAGAATTTCCAACCTATGAATGCTAACTTCGGGTTATTACCATCTTTAGAAACTAGAATTAAAGATaa < 4:386997/141‑1 (MQ=255) gggTGAGGTAGTATTTCCGAGAGAAACAATGATTGGAAGTATGGCTTACTATATTTCTCATGCTAAAAACAATAAGAATTTCCAACCTATGAATGCTAACTTCGGGTTATTACCATCTTTAGAAACTAGAATTAAAGATaa < 1:891385/141‑1 (MQ=255) gTGAGGTAGTATTTCCGAGAGAAACAATGATTGGAAGTATGGCTTACTATATTTCTCATGCTAAAAACAATAAGAATTTCCAACCTATGAATGCTAACTTCGGGTTATTACCATCTTTAGAAACTAGAATTAAAGATaaaa < 2:610165/141‑1 (MQ=255) gAGGTAGTATTTCCGAGAGAAACAATGATTGGAAGTATGGCTTACTATATTTCTCATGCTAAAAACAATAAGAATTTCCAACCTATGAATGCTAACTTCGGGTTATTACCATCTTTAGAAACTAGAATTAAAGATaaaaaa > 3:336346/1‑141 (MQ=255) aGGTAGTATTTCCGAGAGAAACTATGATTGGAAGTATGGCTTACTATATTTCTCATGCTAAAAACAATAAGAATTTCCAACCTATGAATGCTAACTTCGGGTTATTACCATCTTTAGAAACTAGAATTAAAGATAAAAAAg < 3:314616/141‑1 (MQ=255) aGGTAGTATTTCCGAGAGAAACAATGATTGGAAGTATGGCTTACTATATTTCTCATGCTAAAAACAATAAGAATTTCCAACCTATGAATGCTAACTTCGGGTTATTACCATCTTTAGAAACTAGAATTAAAGATAAAAAAg < 1:40839/141‑1 (MQ=255) aGGTAGTATTTCCGAGAGAAACAATGATTGGAAGTATGGCTTACTATATTTCTCATGCTAAAAACAATAAGAATTTCCAACCTATGAATGCTAACTTCGGGTTATTACCATCTTTAGAAACTAGAATTAAAGATAAAAAAg > 2:244687/1‑141 (MQ=255) tagtaTTTCCGAGAGAAACAATGATTGGAAGTATGGCTTACTATATTTCTCATGCTAAAAACAATAAGAATTTCCAACCTATGAATGCTAACTTCGGGTTATTACCATCTTTAGAAACTAGAATTAAAGATAAAAAAGAAc > 2:123874/1‑141 (MQ=255) tagtaTTTCCGAGAGAAACAATGATTGGAAGTATGGCTTACTATATTTCTCATGCTAAAAACAATAAGAATTTCCAACCTATGAATGCTAACTTCGGGTTATTACCATCTTTAGAAACTAGAATTAAAGATAAAAAAGAAc < 4:254598/141‑1 (MQ=255) agtaTTTCCGAGAGAAACAATGATTGGAAGTATGGCTTACTATATTTCTCATGCTAAAAACAATAAGAATTTCCAACCTATGAATGCTAACTTCGGGTTATTACCATCTTTAGAAACTAGAATTAAAGATAAAAAAGAACg < 4:319383/141‑1 (MQ=255) gtaTTTCCGAGAGAAACAATGATTGGAAGTATGGCTTACTATATTTCTCATGCTAAAAACAATAAGAATTTCCAACCTATGAATGCTAACTTCGGGTTATTACCATCTTTAGAAACTAGAATTAAAGATAAAAAAGAACGc > 3:362925/1‑141 (MQ=255) tttCCGAGAGAAACAATGATTGGAAGTATGGCTTACTATATTTCTCATGCTAAAAACAATAAGAATTTCCAACCTATGAATGCTAACTTCGGGTTATTACCATCTTTAGAAACTAGAATTAAAGATAAAAAAGAACGCTAt < 3:401473/141‑1 (MQ=255) ttCCGAGAGAAACAATGATTGGAAGTATGGCTTACTATATTTCTCATGCTAAAAACAATAAGAATTTCCAACCTATGAATGCTAACTTCGGGTTATTACCATCTTTAGAAACTAGAATTAAAGATAAAAAAGAACGCTATg < 2:826812/141‑1 (MQ=255) ttCCGAGAGAAACAATGACTGGAAGTATGGCTTACTATATTTCTCATGCTAAAAACAATAAGAATTTCCAACCTATGAATGCTAACTTCGGGTTATTACCATCTTTAGAACCTAGAATTAAAGATAAAAAAGAACGCTATg > 4:309496/1‑141 (MQ=255) tCCGAGAGAAACTATGATTGGAAGTATGGCTTACTATATTTCTCATGCTAAAAACAATAAGAATTTCCAACCTATGAATGCTAACTTCGGGTTATTACCATCTTTAGAAACTAGAATTAAAGATAAAAAAGAACGCTATGa > 4:404385/1‑141 (MQ=255) tCCGAGAGAAACAATGATTGGAAGTATGGCTTACTATATTTCTCATGCTAAAAACAATAAGAATTTCCAACCTATGAATGCTAACTTCGGGTTATTACCATCTTTAGAAACTAGAATTAAAGATAAAAAAGAACGCTATGa < 1:601337/141‑1 (MQ=255) ccGAGAGAAACAATGATTGGAAGTATGGCTTACTATATTTCTCATGCTAAAAACAATAAGAATTTCCAACCTATGAATGCTAACTTCGGGTTATTACCATCTTTAGAAACTAGAATTAAAGATAAAAAAGAACGCTATGaa > 2:285958/1‑141 (MQ=255) cGAGAGAAACAATGATTGGAAGTATGGCTTAATATATTACTCATGCTAAAAACAATAAGAATTTCCAACCTATGAATGCTAACTTCGGGTTATTACCATCATTAGAAACTAAAATTAAAGAAAAAAAAGAACACTATGAAg > 4:154533/1‑141 (MQ=255) agagaAACAATGATTGGAAGTATGGCTTACTATATTTCTCATGCTAAAAACAATAAGAATTTCCAACCTATGAATGCTAACTTCGGGTTATTACCATCTTTAGAAACTAGAATTAAAGATAAAAAAGAACGCTATGAAGca > 2:171685/1‑141 (MQ=255) gagaAACAATGATTGGAAGTATGGCTTACTATATTTCTCATGCTAAAAACAATAAGAATTTCCAACCTATGAATGCTAACTTCGGGTTATTACCATCTTTAGAAACTAGAATAAAAGATAAAAAAGAACGCTATGAAGcac > 2:302580/1‑141 (MQ=255) aCAATGATTGGAAGTATGGCTTACTATATTTCTCATGCTAAAAACAATAAGAATTTCCAACCTATGAATGCTAACTTCGGGTTATTACCATCTTTAGAAACTAGAATTAAAGATAAAAAAGAACGCTATGAAGCACAAGCt > 1:375056/1‑141 (MQ=255) cAATGATTGGAAGTATGGCTTACTATATTTCTCATGCTAAAAACAATAAGAATTTCCAACCTATGAATGCTAACTTCGGGTTATTACCATCTTTAGAAACTAGAATTAAAGATAAAAAAGAACGCTATGAAGCACAAGCTa < 1:435479/141‑1 (MQ=255) aaTGATTGGAAGTATGGCTTACTATATTTCTCATGCTAAAAACAATAAGAATTTCCAACCTATGAATGCTAACTTCGGGTTATTACCATCTTTAGAAACTAGAATTAAAGATAAAAAAGAACGCTATGAAGCACAAGCTaa > 2:383325/1‑141 (MQ=255) aaTGATTGGAAGTATGGCTTACTATATTTCTCATGCTAAAAACAATAAGAATTTCCAACCTATGAATGCTAACTTCGGGTTATTACCATCTTTAGAAACTAGAATTAAAGATAAAAAAGAACGCTATGAAGCACAAGCTaa < 2:505009/141‑1 (MQ=255) aaTGATTGGAAGTATGGCTTACTATATTTCTCATGCTAAAAACAATAAGAATTTCCAACCTATGAATGCTAACTTCGGGTTATTACCATCTTTAGAAACTAGAATTAAAGATAAAAAAGAACGCTATGAAGCACAAGCTaa > 3:195880/1‑141 (MQ=255) | TCACAACCAAACATACAGTTTGCGGGACAAATGACTGGTGTTGAAGGTTATGTAGAAAGCGCAGCTAGCGGCTTAGTTGCAGGTATCAATCTTGCGCATAAAATATTAGGCAAGGGTGAGGTAGTATTTCCGAGAGAAACAATGATTGGAAGTATGGCTTACTATATTTCTCATGCTAAAAACAATAAGAATTTCCAACCTATGAATGCTAACTTCGGGTTATTACCATCTTTAGAAACTAGAATTAAAGATAAAAAAGAACGCTATGAAGCACAAGCTAA > USA300TCH1516_ALE/1269926‑1270206 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |