BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	1,299,677	C→T	5.2%	G446G (GGC→GGT)	rnj2 →	Ribonuclease J 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,299,677	0	C	T	5.2%	419.0 / 7.6	133	G446G (GGC→GGT)	rnj2	Ribonuclease J 2
Reads supporting (aligned to +/- strand): ref base C (74/52); new base T (5/2); total (79/54)
Fisher's exact test for biased strand distribution p-value = 7.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.07e-01

Only 100 of 135 total aligned reads displayed.

GCACATGCGAAGCTAGCAGCTGAAGCAGGTGTTGCACCAGAAAAGATTTTCCTTGTGGAAAAAGGAGATGTCATTAATTACAACGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTT  >  USA300TCH1516_ALE/1299537‑1299817

gCACATGCGAAGCTAGCAGCTGAAGCAGGTGTTGCACCAGAAAAGATTTTCCTTGTGGAAAAAGGAGATGTCATTAATTACAACGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGc                                                                                                                                              >  1:700521/1‑141 (MQ=255)

  acaTGCGAAGCTAGCAGCTGAAGCAGGTGTTGCACCAGAAAAGATTTTCCTTGTGGAAAAAGGAGATGTCATTAATTACAACGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCAt                                                                                                                                            <  3:434471/141‑1 (MQ=255)

   caTGCGAAGCTAGCAGCTGAAGCAGGTGTTGCAAAAGAAAAGATTTTCCTTGTGAAAAAAGGAGATTTCATTAATTACAACGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATTGCagt                                                                                                                                           <  4:343228/141‑3 (MQ=255)

     agCGAAGCTAGCAGCTGAAGCAGGTGTTGCACCAGAAAAGATTTAAAAAAAAAAAAAAGGAGATGTCATTAATTACAACGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTgg                                                                                                                                         <  2:967494/140‑1 (MQ=255)

        gAAGCTAGCAGCTGAAGCAGGTGTTGCACCAGAAAAGATTTTCCTTGTGGAAAAAGGAGATGTCATTAATTACAACGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTAt                                                                                                                                      >  4:141614/1‑141 (MQ=255)

          aGCTAGCAGCTGAAGCAGGTGTTGCACCAGAAAAGATTTTCCTTGTGGAAAAAGGAGATGTCATTAATTACAACGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTg                                                                                                                                    >  1:1074644/1‑141 (MQ=255)

          aGCTAGCAGCTGAAGCAGGTGTTGCACCAGAAAAGATTTTCCTTGTGGAAAAAGGAGATGTCATTAATTACAACGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTg                                                                                                                                    >  2:579346/1‑141 (MQ=255)

                cagcTGAAGCAGGTGTTGCATCGGAAAAGATTTTCCTTGTGGAAAAAGGAGATGTCATTAATTACAACGGTAAAGATATGGTATTATGTTAATGGGTTAGGTCGTGAAATATTTTAATAGATGGTATTGGTATTGGGGATg                                                                                                                              <  2:762686/141‑1 (MQ=255)

                  gcTGAAGCAGGTGTTGCACCAGAAAAGATTTTCCTTGTGGAAAAAGGAGATGTCATTAATTACAACGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTa                                                                                                                            <  1:755295/141‑1 (MQ=255)

                    tGAAGCAGGTGTTGCACCAGAAAAGATTTTCCTTGTGGAAAAAGGAGATGTCATTAATTACAACGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAgg                                                                                                                          <  2:928330/141‑1 (MQ=255)

                    tGAAGCAGGTGTTGCACCAGAAAAGATTTTCCTTGTGGAAAAAGGAGATGTCATTAATTACAACGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAgg                                                                                                                          <  1:435858/141‑1 (MQ=255)

                     gAAGCAGGTGTTGCACCAGAAAAGATTTTCCTTGTGGAAAAAGGAGATGTCATTAATTACAACGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGa                                                                                                                         <  2:375027/141‑1 (MQ=255)

                     gAAGCAGGTGTTGCACCAGAAAAGATTTTCCTTGTGGAAAAAGGAGATGTCATTAATTACAACGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGa                                                                                                                         <  1:343607/141‑1 (MQ=255)

                      aaGCAGGTGTTGCACCAGAAAAGATTTTCCTTGTGG‑AAAAGGAGATGTCATTAATTACAACGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGaaa                                                                                                                       <  1:168887/141‑1 (MQ=255)

                       aGCAGGTGTTGCACCAGAAAAGATTTTCCTTGTGGAAAAAGGAGATGTCATTAATTACAACGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGaaa                                                                                                                       <  2:987275/141‑1 (MQ=255)

                        gCAGGTGTTGCACCAGAAAAGATTTTCCTTGTGGAAAAAGGAGATGTCATTAATTACAACGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAat                                                                                                                      >  4:421707/1‑141 (MQ=255)

                         gaGGGGTTGCACCAGAAAAGATTTTCCTTGTGGAAAAGGGAGATGTCAATAATTACAACGGTAAAGATATGATATTAAATGGAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAata                                                                                                                     <  4:324059/140‑1 (MQ=255)

                         cAGGTGTTGCACCAGAAAAGATTTTCCTTGTGGAAAAAGGAGATGTCATTAATTACAACGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAata                                                                                                                     >  2:326504/1‑141 (MQ=255)

                         cAGGTGTTGCACCAGAAAAGATTTTCCTTGTGGAAAAAGGAGATGTCATTAATTACAACGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAata                                                                                                                     <  1:251271/141‑1 (MQ=255)

                         cAGGTGTTGCACCAGAAAAGATTTTCCTTGTGGAAAAAGGAGATGTCATTAATTACAACGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAata                                                                                                                     <  1:240824/141‑1 (MQ=255)

                           ggTGTTGCACCAGAAAAGATTTTCCTTGTGGAAAAAGGAGATGTCATTAATTACAACGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATc                                                                                                                   >  4:61687/1‑141 (MQ=255)

                           ggTGTTGCACCAGAAAAGATTTTCCTTGTGGAAAAAGGAGATGTCATTAATTACAACGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATc                                                                                                                   >  1:479044/1‑141 (MQ=255)

                            gtgtTGCACCAGAAAAGATTTTCCTTGTGGAAAAAGGAGATGTCATTAATTACAACGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCg                                                                                                                  >  3:39822/1‑141 (MQ=255)

                            gtgtTGCACCAGAAAAGATTTTCCTTGTGGAAAAAGGAGATGTCATTAATTACAACGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCg                                                                                                                  <  3:395158/141‑1 (MQ=255)

                               ttGCACCAGAAAAGATTTTCCTTGTGGAAAAAGGAGATGTCATTAATTACAACGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCgtgt                                                                                                               >  4:105188/1‑141 (MQ=255)

                               ttGCACCAGAAAAGATTTTCCTTGTGGAAAAAGGAGATGTCATTAATTACAACGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCgtgt                                                                                                               <  2:275349/141‑1 (MQ=255)

                                 gCACCAGAAAAGATTTTCCTTGTGGAAAAAGGAGATGTCATTAATTACAACGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTg                                                                                                             >  2:847938/1‑141 (MQ=255)

                                 gCACCAGAAAAGATTTTCCTTGTGGAAAAAGGAGATGTCATTAATTACAACGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTg                                                                                                             >  2:183445/1‑141 (MQ=255)

                                 gCACCAGAAAAGATTTTCCTTGTGGAAAAAGGAGATGTCATTAATTACAACGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTg                                                                                                             >  3:47389/1‑141 (MQ=255)

                                  cACCAGAAAAGATTTTCCTTGTGGAAAAAGGAGATGTCATTAATTACAACGGTAAAGATATGATATTAAATGAAAAGGTAAATTCATGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTga                                                                                                            <  1:20584/141‑1 (MQ=255)

                                  cACCAGAAAAGATTGTCCGTGTGGAAAAAGAAGATGTCATTAATTACAACGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTga                                                                                                            <  4:367495/141‑1 (MQ=255)

                                      aGAAAAGATTTTCCTTGTGGAAAAAGGAGATGTCATTAATTACAACGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTgagaga                                                                                                        <  3:381677/141‑1 (MQ=255)

                                       gAAAAGATTTTCCTTGTGGAAAAAGGAGATGTCATTAATTACAACGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGAc                                                                                                       >  2:995339/1‑141 (MQ=255)

                                        aaaaGGTTTTCCTTGTGGAAAAAGGAGATGTCATTAATTACAACGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGAcc                                                                                                      <  2:1004069/141‑1 (MQ=255)

                                        aaaaGATTTTCCTTGTGGAAAAAGGAGATGTCATTAATTACAACGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGAcc                                                                                                      <  1:547642/141‑1 (MQ=255)

                                        aaaaGATTTTCCTTGTGGAAAAAGGAGATGTCATTAATTACAACGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGAcc                                                                                                      <  1:497762/141‑1 (MQ=255)

                                            gATTTTCCTTGTGGAAAAAGGAGATGTCATTAATTACAACGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCa                                                                                                  >  1:849823/1‑141 (MQ=255)

                                            gATTTTCCTTGTGGAAAAAGGAGATGTCATTAATTACAACGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCa                                                                                                  >  2:952235/1‑141 (MQ=255)

                                              ttttCCTTGTGGAAAAAGGAGATGTCATTAATTACAACGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATc                                                                                                >  3:162869/1‑141 (MQ=255)

                                              ttttCCTTGTGGAAAAAGGAGATGTCATTAATTACAACGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATc                                                                                                >  3:307452/1‑141 (MQ=255)

                                              ttttCCTTGTGGAAAAAGGAGATGTCATTAATTACAACGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAAAAAAAAGTTGAGAGACCGTCATc                                                                                                >  1:283193/1‑141 (MQ=255)

                                                 tccacaaggAAAAAGGAGATGTCATTAATTACAACGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCttt                                                                                             >  2:283423/8‑141 (MQ=255)

                                                  ccTTGTGGAAAAAGGAGATGTCATTAATTACAACGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCtttt                                                                                            <  1:321781/141‑1 (MQ=255)

                                                    ttGTGGAAAAAGGAGATGTCATTAATTACAACGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAg                                                                                          <  3:7194/141‑1 (MQ=255)

                                                      gtgGAAAAAGGAGATGTCATTAATTACAACGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCGTTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCa                                                                                        <  2:47609/141‑1 (MQ=255)

                                                      gtgGAAAAAGGAGATGTCATTAATTACAACGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCa                                                                                        >  1:393376/1‑141 (MQ=255)

                                                      gtgGAAAAAGGAGATGTCATTAATTACAACGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCa                                                                                        <  2:47665/141‑1 (MQ=255)

                                                       tgGAAAAAGGAGATGTCATTAATTACAACGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCag                                                                                       >  2:1054718/1‑141 (MQ=255)

                                                             aaGGAGATGTCATTAATTACAACGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATg                                                                                 >  1:146511/1‑141 (MQ=255)

                                                             aaGGAGATGTCATTAATTACAACGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATg                                                                                 >  1:205148/1‑141 (MQ=255)

                                                             aaGGAGATGTCATTAATTACAACGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAAATGGCATAGGAATTGGGGAAGTCGAAATTGATGAAGTGATAGACGGTCAGCGTTTATCAGAAGATg                                                                                 >  3:315374/1‑141 (MQ=255)

                                                                gagaTGTCATTAATTACAACGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTa                                                                              <  2:79811/141‑1 (MQ=255)

                                                                 agaTGTCATTAATTACAACGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTAt                                                                             >  3:448336/1‑141 (MQ=255)

                                                                 agaTGTCATTAATTACAACGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTAt                                                                             >  1:726652/1‑141 (MQ=255)

                                                                  gaTGTCATTAATTACAACGGTAAAGATATGATATTAAATGAGAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATCGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGTAGATGGTATc                                                                            <  2:995168/141‑1 (MQ=255)

                                                                  gaTGTCATTAATTACAACGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATc                                                                            >  2:396865/1‑141 (MQ=255)

                                                                  gaTGTCATTAATTACAACGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATc                                                                            <  1:500896/141‑1 (MQ=255)

                                                                    tGTCATTAATTACAACGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCtt                                                                          <  1:579346/141‑1 (MQ=255)

                                                                     gTCATTAATTACAACGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCttt                                                                         >  2:750410/1‑141 (MQ=255)

                                                                     gTCATGAATTACAGCGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCttt                                                                         <  2:909611/141‑1 (MQ=255)

                                                                         ttaattaCAACGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTg                                                                     >  1:1034400/1‑141 (MQ=255)

                                                                             ttaCAACGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCtgt                                                                 >  1:136966/1‑141 (MQ=255)

                                                                               aCAACGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGATCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCtgttg                                                               >  2:826742/1‑141 (MQ=255)

                                                                                cAACGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCtgttgt                                                              <  1:697861/141‑1 (MQ=255)

                                                                                 aaCGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCtgttgga                                                             <  4:446620/141‑3 (MQ=255)

                                                                                 aaCGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTa                                                             >  2:877971/1‑141 (MQ=255)

                                                                                 aaCGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTa                                                             >  1:372266/1‑141 (MQ=255)

                                                                                    ggTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACg                                                          <  2:302163/141‑1 (MQ=255)

                                                                                     gTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGt                                                         <  2:781333/141‑1 (MQ=255)

                                                                                      tAAAGATATGATATTAAATGAAAAGGTAAATTCGGGAAATATTTTAATAGATGGTATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTAGTAACGtt                                                        <  1:695134/141‑1 (MQ=255)

                                                                                      tAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGtt                                                        <  2:212844/141‑1 (MQ=255)

                                                                                         aGATATGATATTAAATGAAAAGGTAAATTCGGGAAATATTTTAATAGATGGTATTGGTATTGGGGATGTAGGAAAGAGCGTGTGGATAGACCGGCATCTTTGAGCAGAAGATGGTATCGTTATTGCTGTAGTAACGTTAGa                                                     >  1:918826/1‑141 (MQ=255)

                                                                                          gatatgatatTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGAt                                                    >  4:82744/1‑141 (MQ=255)

                                                                                          gatatgatatTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGAt                                                    >  2:567149/1‑141 (MQ=255)

                                                                                              tgatatTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTa                                                >  4:442840/1‑141 (MQ=255)

                                                                                                   ttAAATGAAAAGGTAAATTCGGGAAATATTTTAATAGATGGTATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTAGTAACGTTAGATCCTAAAAAt                                           >  2:53627/1‑141 (MQ=255)

                                                                                                   ttAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAAt                                           <  3:170828/141‑1 (MQ=255)

                                                                                                      aaTGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGa                                        >  2:718847/1‑141 (MQ=255)

                                                                                                       aTGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGAc                                       >  3:191429/1‑141 (MQ=255)

                                                                                                             aGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAg                                 <  4:188096/141‑1 (MQ=255)

                                                                                                             aGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAg                                 >  2:836729/1‑141 (MQ=255)

                                                                                                              ggTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGc                                >  1:922623/1‑141 (MQ=255)

                                                                                                                  aaTTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCg                            >  3:242423/1‑141 (MQ=255)

                                                                                                                    ttCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCggg                          >  4:393074/1‑141 (MQ=255)

                                                                                                                     tCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGa                         <  3:26638/141‑1 (MQ=255)

                                                                                                                      cgggAAATATTTTAATAGATGGTATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTAGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCgggtc                        >  1:380776/3‑139 (MQ=255)

                                                                                                                      cAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGAACCTAAAAATAGACGTATAGCTGCGGGAc                        <  2:1085153/141‑1 (MQ=255)

                                                                                                                       aGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGAcc                       <  4:242423/141‑1 (MQ=255)

                                                                                                                        ggAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCt                      >  3:224655/1‑141 (MQ=255)

                                                                                                                        ggAAATATTTTAATAGATGGCATTGGTATTAGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCGTAAAAATCTACGTATAGCTGCGGGACCt                      >  2:728062/1‑141 (MQ=255)

                                                                                                                           aaTATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGaa                   >  2:4802/1‑141 (MQ=255)

                                                                                                                              atTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAAtt                >  1:276939/1‑141 (MQ=255)

                                                                                                                                tttAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCa              <  2:922623/141‑1 (MQ=255)

                                                                                                                                 ttAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCaa             >  1:379062/1‑141 (MQ=255)

                                                                                                                                  tAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAAt            >  1:809542/1‑141 (MQ=255)

                                                                                                                                    aTAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAAtct          >  1:295118/1‑141 (MQ=255)

                                                                                                                                    aTAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAAtct          >  1:1014618/1‑141 (MQ=255)

                                                                                                                                      aGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCg        <  2:888301/141‑1 (MQ=255)

                                                                                                                                        aTGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTg      <  1:54198/141‑1 (MQ=255)

                                                                                                                                        aTGCCATTGGTATCGGGGATGTCGGCAATATCGTGTTCAGCGGCCATCATCTCTTACCCGACGCTGGAACACATATTGTTAATGTACCGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTg      <  3:154754/141‑1 (MQ=255)

GCACATGCGAAGCTAGCAGCTGAAGCAGGTGTTGCACCAGAAAAGATTTTCCTTGTGGAAAAAGGAGATGTCATTAATTACAACGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTT  >  USA300TCH1516_ALE/1299537‑1299817

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`