BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	1,299,755	T→A	6.4%	V472V (GTT→GTA)	rnj2 →	Ribonuclease J 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,299,755	0	T	A	6.4%	352.9 / 11.4	111	V472V (GTT→GTA)	rnj2	Ribonuclease J 2
Reads supporting (aligned to +/- strand): ref base T (50/53); new base A (5/2); total (55/56)
Fisher's exact test for biased strand distribution p-value = 4.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

Only 100 of 116 total aligned reads displayed.

ACAACGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGACTTATTACGTGAAGCAGAAGAGAAAGTACGTGAAATAGTAGAGGCTGGTTTAC  >  USA300TCH1516_ALE/1299616‑1299894

aCAACGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGATCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCtgttg                                                                                                                                            >  2:826742/1‑141 (MQ=255)

 cAACGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCtgttgt                                                                                                                                           <  1:697861/141‑1 (MQ=255)

  aaCGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCtgttgga                                                                                                                                          <  4:446620/141‑3 (MQ=255)

  aaCGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTa                                                                                                                                          >  2:877971/1‑141 (MQ=255)

  aaCGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTa                                                                                                                                          >  1:372266/1‑141 (MQ=255)

   aCGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTaa                                                                                                                                         >  2:90156/1‑141 (MQ=255)

     ggTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACg                                                                                                                                       <  2:302163/141‑1 (MQ=255)

      gTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGt                                                                                                                                      <  2:781333/141‑1 (MQ=255)

       tAAAGATATGATATTAAATGAAAAGGTAAATTCGGGAAATATTTTAATAGATGGTATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTAGTAACGtt                                                                                                                                     <  1:695134/141‑1 (MQ=255)

       tAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGtt                                                                                                                                     <  2:212844/141‑1 (MQ=255)

          aGATATGATATTAAATGAAAAGGTAAATTCGGGAAATATTTTAATAGATGGTATTGGTATTGGGGATGTAGGAAAGAGCGTGTGGATAGACCGGCATCTTTGAGCAGAAGATGGTATCGTTATTGCTGTAGTAACGTTAGa                                                                                                                                  >  1:918826/1‑141 (MQ=255)

           gatatgatatTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGAt                                                                                                                                 >  4:82744/1‑141 (MQ=255)

           gatatgatatTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGAt                                                                                                                                 >  2:567149/1‑141 (MQ=255)

               tgatatTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTa                                                                                                                             >  4:442840/1‑141 (MQ=255)

                    ttAAATGAAAAGGTAAATTCGGGAAATATTTTAATAGATGGTATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTAGTAACGTTAGATCCTAAAAAt                                                                                                                        >  2:53627/1‑141 (MQ=255)

                    ttAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAAt                                                                                                                        <  3:170828/141‑1 (MQ=255)

                       aaTGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGa                                                                                                                     >  2:718847/1‑141 (MQ=255)

                       aaTGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGa                                                                                                                     >  1:467402/1‑141 (MQ=255)

                        aTGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGAc                                                                                                                    >  3:191429/1‑141 (MQ=255)

                              aGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAg                                                                                                              >  2:836729/1‑141 (MQ=255)

                                   aaTTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCg                                                                                                         >  1:531378/1‑141 (MQ=255)

                                   aaTTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCg                                                                                                         >  3:242423/1‑141 (MQ=255)

                                    aTTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCgg                                                                                                        >  1:120470/1‑141 (MQ=255)

                                    aTTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCgg                                                                                                        >  3:213300/1‑141 (MQ=255)

                                     ttCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCggg                                                                                                       >  4:393085/1‑141 (MQ=255)

                                     ttCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCggg                                                                                                       >  4:393074/1‑141 (MQ=255)

                                      tCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGa                                                                                                      <  3:26638/141‑1 (MQ=255)

                                       cgggAAATATTTTAATAGATGGTATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTAGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCgggtc                                                                                                     >  1:380867/3‑139 (MQ=255)

                                       cAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGAACCTAAAAATAGACGTATAGCTGCGGGAc                                                                                                     <  2:1085153/141‑1 (MQ=255)

                                        aGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGAcc                                                                                                    <  4:242423/141‑1 (MQ=255)

                                         ggAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGGTGGTTATTTTGTTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCt                                                                                                   >  1:613788/1‑141 (MQ=255)

                                         ggAAATATTTTAATAGATGGCATTGGTATTAGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCGTAAAAATCTACGTATAGCTGCGGGACCt                                                                                                   >  2:728062/1‑141 (MQ=255)

                                              tatTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAAt                                                                                              >  2:635619/1‑141 (MQ=255)

                                               atTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAAtt                                                                                             >  1:276939/1‑141 (MQ=255)

                                                 tttAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCa                                                                                           <  2:922623/141‑1 (MQ=255)

                                                  ttAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCaa                                                                                          >  1:379062/1‑141 (MQ=255)

                                                   tAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAAt                                                                                         >  1:809542/1‑141 (MQ=255)

                                                     aTAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAAtct                                                                                       >  1:1014618/1‑141 (MQ=255)

                                                     aTAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAAtct                                                                                       >  1:295118/1‑141 (MQ=255)

                                                       aGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCg                                                                                     <  2:888301/141‑1 (MQ=255)

                                                         aTGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTg                                                                                   <  1:54198/141‑1 (MQ=255)

                                                         aTGCCATTGGTATCGGGGATGTCGGCAATATCGTGTTCAGCGGCCATCATCTCTTACCCGACGCTGGAACACATATTGTTAATGTACCGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTg                                                                                   <  3:154754/141‑1 (MQ=255)

                                                             cATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGtt                                                                               >  3:26644/1‑141 (MQ=255)

                                                               ttGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTg                                                                             <  1:77516/141‑1 (MQ=255)

                                                                     ttGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATg                                                                       <  2:159481/141‑1 (MQ=255)

                                                                      tGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGt                                                                      <  1:462644/141‑1 (MQ=255)

                                                                        gggATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTAc                                                                    <  1:183445/141‑1 (MQ=255)

                                                                         ggATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGAGGGTATCTTTAGTGCTGTTGTAACGTTAGATCCTAAAAATAGTCGTATAGCTGCGGGACCTGGAATTCAATCGCGTGGGTTTGTATATGTACg                                                                   <  2:976211/141‑1 (MQ=255)

                                                                          gATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGt                                                                  <  1:90156/141‑1 (MQ=255)

                                                                           aTGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTg                                                                 >  2:622352/1‑141 (MQ=255)

                                                                            tGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGa                                                                <  2:818513/141‑1 (MQ=255)

                                                                             gTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGaa                                                               <  1:73612/141‑1 (MQ=255)

                                                                               aGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAg                                                             <  3:206196/141‑1 (MQ=255)

                                                                                ggAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGt                                                            >  4:326915/1‑141 (MQ=255)

                                                                                ggAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGt                                                            >  3:2306/1‑141 (MQ=255)

                                                                                  aaaTATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGa                                                          >  3:262694/1‑141 (MQ=255)

                                                                                      atCGTGTTGAGAGACCGTTAGCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGAc                                                      <  3:115585/141‑1 (MQ=255)

                                                                                      atCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTCTTGCTGACGTGACAACAGATCCTAATAATAAACGTATAGCTGCGGTACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGAc                                                      <  1:758328/141‑1 (MQ=255)

                                                                                      atCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGAc                                                      <  1:1004346/141‑1 (MQ=255)

                                                                                      atCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGAc                                                      >  4:10355/1‑141 (MQ=255)

                                                                                        cGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGAAACTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGACtt                                                    <  2:301335/141‑1 (MQ=255)

                                                                                         gtgtTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGACtta                                                   >  2:637337/1‑141 (MQ=255)

                                                                                         gtgtTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGACtta                                                   >  2:323607/1‑141 (MQ=255)

                                                                                          tgtTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGACttat                                                  <  1:306588/141‑1 (MQ=255)

                                                                                           tttGAGAGACCGTCATCTTTTAGCAGAAGATGGTAGCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGACttatt                                                 <  1:455941/140‑1 (MQ=255)

                                                                                              gagagaCCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGACTTATTACg                                              <  3:436681/141‑1 (MQ=255)

                                                                                                gagaCCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGACTTATTACGTg                                            >  2:88752/1‑141 (MQ=255)

                                                                                                  gaCCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGACTTATTACGTGaa                                          >  2:1063615/1‑141 (MQ=255)

                                                                                                  gaCCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGACTTATTACGTGaa                                          <  3:270915/141‑1 (MQ=255)

                                                                                                   aCCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGACTTATTACGTGAAg                                         <  1:675611/141‑1 (MQ=255)

                                                                                                    ccGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGACTTATTACGTGAAGc                                        >  3:375865/1‑141 (MQ=255)

                                                                                                    ccGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGACTTATTACGTGAAGc                                        >  1:764430/1‑141 (MQ=255)

                                                                                                     cGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGACTTATTACGTGAAGCa                                       <  1:821044/141‑1 (MQ=255)

                                                                                                         aTCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGACTTATTACGTGAAGCagaag                                   <  3:61687/141‑1 (MQ=255)

                                                                                                         aTCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGACTTATTACGTGAAGCagaag                                   <  2:479044/141‑1 (MQ=255)

                                                                                                           cTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGACTTATTACGTGAAGCAGAagag                                 <  4:307452/141‑1 (MQ=255)

                                                                                                           cTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGACTTATTACGTGAAGCAGAagag                                 <  1:561750/141‑1 (MQ=255)

                                                                                                            ttttAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGCCCTGAAATTGAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGACTTATTACGTGAAGCAGAAgaga                                >  1:1028049/1‑141 (MQ=255)

                                                                                                            ttttAGCAGAAGATGGTATCTGTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGACTTATTACGTGAAGCAGAAgaga                                <  2:702147/141‑1 (MQ=255)

                                                                                                              ttAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTACAGCCGCGGGACGTGAAAACCAATCTCGGGGGTTTTAATATGTACGTGAAAGTGAAGTCTGATGACGTGAAGCAGAAGAGaaa                              >  1:918574/1‑141 (MQ=255)

                                                                                                              ctgctaaaagaTGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGACTTATTACGTGAAGCAGAAGAGaaa                              >  1:114497/8‑141 (MQ=255)

                                                                                                               tAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGACTTATTACGTGAAGCAGAAGAGAAAg                             <  3:296159/141‑1 (MQ=255)

                                                                                                               tAGCAGAAGATGGTATCTTTATTGCTGTAGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGTCCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGACTTATTACGTGAAGCAGAAGAGAAAg                             <  1:45994/141‑1 (MQ=255)

                                                                                                                 gCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGACTTATTACGTGAAGCAGAAGAGAAAGTa                           <  2:1074644/141‑1 (MQ=255)

                                                                                                                   agaagaTGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGACTTATTACGTGAAGCAGAAGAGAAAGTACg                         >  4:432627/1‑141 (MQ=255)

                                                                                                                     tataTGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGACTTATTACGTGAAGCAGAAGAGAAAGTACGTg                       <  2:283193/138‑1 (MQ=255)

                                                                                                                     aagaTGGTATCTTTATTGCTGTTGTAAAAATAGAAAATAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGACTTATTACGTGAAGCAGAAGAGAAAGTACGTg                       <  2:283324/141‑1 (MQ=255)

                                                                                                                          ggTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGACTTATTACGTGAAGCAGAAGAGAAAGTACGTGAAAta                  >  1:309806/1‑141 (MQ=255)

                                                                                                                           gTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGACTTATTACGTGAAGCAGAAGAGAAAGTACGTGAAAtag                 <  1:850744/141‑1 (MQ=255)

                                                                                                                           gTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGACTTATTACGTGAAGCAGAAGAGAAAGTACGTGAAAtag                 <  4:4984/141‑1 (MQ=255)

                                                                                                                             aTCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGACTTATTACGTGAAGCAGAAGAGAAAGTACGTGAAAtagta               >  1:86190/1‑141 (MQ=255)

                                                                                                                              tCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGACTTATTACGTGAAGCAGAAGAGAAAGTACGTGAAAtagtag              <  4:158821/141‑1 (MQ=255)

                                                                                                                               cTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGACTTATTACGTGAAGCAGAAGAGAAAGTACGTGAAATAGTaga             <  2:1034400/141‑1 (MQ=255)

                                                                                                                               cTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGACTTATTACGTGAAGCAGAAGAGAAAGTACGTGAAATAGTaga             <  2:543846/141‑1 (MQ=255)

                                                                                                                                tttATTGCTGTTGTAAGGTTAGTTCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGTGTTTGTATTTGTACGTGAAAGTGAAGACTTATTACGTGAAGCAGAAGAGAAAGTACGTGAAATAGTagag            <  1:454113/141‑1 (MQ=255)

                                                                                                                                  tATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGACTTATTACGTGAAGCAGAAGAGAAAGTACGTGAAATAGTAGAGGc          <  4:39822/141‑1 (MQ=255)

                                                                                                                                       cTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGACTTATTACGTGAAGCAGAAGAGAAAGTACGTGAAATAGTAGAGGCTGGtt     <  4:440718/141‑1 (MQ=255)

                                                                                                                                       cTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGACTTATTACGTGAAGCAGAAGAGAAAGTACGTGAAATAGTAGAGGCTGGtt     >  1:1058143/1‑141 (MQ=255)

                                                                                                                                       cTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGACTTATTACGTGAAGCAGAAGAGAAAGTACGTGAAATAGTAGAGGCTGGtt     <  3:421707/141‑1 (MQ=255)

                                                                                                                                         gttgtAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGACTTATTACGTGAAGCAGAAGAGAAAGTACGTGAAATAGTAGAGGCTGGTTTa   >  1:920028/1‑141 (MQ=255)

ACAACGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGACTTATTACGTGAAGCAGAAGAGAAAGTACGTGAAATAGTAGAGGCTGGTTTAC  >  USA300TCH1516_ALE/1299616‑1299894

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`