Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 1,306,597 T→C 6.2% S220S (AGT→AGC)  phaB → Acetoacetyl‑CoA reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,306,5970TC 6.2% 305.4 / 7.7 108S220S (AGT→AGC) phaBAcetoacetyl‑CoA reductase
Reads supporting (aligned to +/- strand):  ref base T (66/35);  new base C (3/4);  total (69/39)
Fisher's exact test for biased strand distribution p-value = 2.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 112 total aligned reads displayed.

TTTGTAGCCGGTAATATGGCAAGTGAGTGGCAAGAAGATGAACTTCAAGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTATGATTGATGTTATAATATGAATATTACAATGAATCATAATTTGTTAATTAAACAAGGGGTG  >  USA300TCH1516_ALE/1306457‑1306737
                                                                                                                                            |                                                                                                                                            
tttGTAGCCGGTAATATGGCAAGTGAGTGGCAAGAAGATGAACTTCAAGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGCGCGCGCCTCCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAgt                                                                                                                                              >  1:284392/1‑141 (MQ=255)
 ttGTAGCCGGTAATATGGCAAGTGAGTGGCAAGAAGATGAACTTCAAGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAgtg                                                                                                                                             <  4:177304/141‑1 (MQ=255)
  tGTAGCCGGTAATATGGCAAGTGAGTGGCAAGAAGATGAACTTCAAGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAgtgt                                                                                                                                            <  1:765980/141‑1 (MQ=255)
    tAGCCGGTAATATGGCAAGTGAGTGGCAAGAAGATGAACTTCAAGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTa                                                                                                                                          >  1:540848/1‑141 (MQ=255)
    tAGCCGGTAATATGGCAAGTGAGTGGCAAGAAGATGAACTTCAAGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTa                                                                                                                                          <  1:936843/141‑1 (MQ=255)
    tAGCCGGTAATATGGCAAGGGAGTGGCAAGAAGATGAACTTCAAGCAATGACAACTGAGTTACCACAACAGCGAATGGCTTTACCGAGTGAGGTTGCTCATAAATGGGCCTATGTATATCACTCAATAGCTAGAAGCGTTa                                                                                                                                          >  2:684482/1‑141 (MQ=255)
             atatGGCAAGTGAGTGGCAAGAAGATGAACTTCAAGCAATGATAACTGACTTACCACAACAGCGAGTGGGTGTACCGAGTGTGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACta                                                                                                                                 >  1:319457/1‑141 (MQ=255)
             atatGGCAAGTGAGTGGCAAGAAGATGAACTTCAAGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACta                                                                                                                                 >  2:973600/1‑141 (MQ=255)
               gtGGCAAGTGAGTGGCAAGAAGATGAACTTCAAGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACTCAAATGCTAGAAGCGTTACTGGAACtata                                                                                                                               >  4:442267/2‑141 (MQ=255)
               atGGCAAGTGAGTGGCAAGAAGATGAACTTCAAGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACtata                                                                                                                               >  3:424364/1‑141 (MQ=255)
               atGGCAAGTGAGTGGCAAGAAGATGAACTTCAAGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACtata                                                                                                                               >  3:159852/1‑141 (MQ=255)
                tGGCAAGTGAGTGGCAAGAAGATGAACTTCAAGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACACGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATAc                                                                                                                              >  4:395999/1‑141 (MQ=255)
                 ggCAAGTGAGTGGCAAGAAGATGAACTTCAAGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACa                                                                                                                             >  2:567712/1‑141 (MQ=255)
                 ggCAAGTGAGTGGCAAGAAGATGAACTTCAAGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACa                                                                                                                             <  1:380711/141‑1 (MQ=255)
                  gCAAGTGAGTGGCAAGAAGATGAACTTCAAGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAg                                                                                                                            <  2:944064/141‑1 (MQ=255)
                     agtgagtgGCAAGAAGATGAACTTCAAGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGaaa                                                                                                                         <  1:790453/141‑1 (MQ=255)
                      gtgagtgGCAAGAAGATGAACTTCAAGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAg                                                                                                                        >  2:969494/1‑141 (MQ=255)
                        gagtgGCAAGAAGATGAACTTCAAGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGtt                                                                                                                      >  2:108504/1‑141 (MQ=255)
                         agtgGCAAGAAGATGAACTTCAAGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTa                                                                                                                     <  2:170679/141‑1 (MQ=255)
                         agtgGCAAGAAGATGAACTTCAAGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTa                                                                                                                     <  4:106880/141‑1 (MQ=255)
                                agaagaTGAACTTCAAGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGc                                                                                                              <  4:287526/141‑1 (MQ=255)
                                      tGAACTTCAAGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGta                                                                                                        >  1:57012/1‑141 (MQ=255)
                                       gAACTTCAAGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGtat                                                                                                       >  2:190013/1‑141 (MQ=255)
                                        aaCTTCAAGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACTCAAATGCTAGAAGCGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGtata                                                                                                      <  1:677556/141‑1 (MQ=255)
                                        aaCTTCAAGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGtata                                                                                                      >  2:1019494/1‑141 (MQ=255)
                                        aaCTTCAAGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGtata                                                                                                      <  1:15042/141‑1 (MQ=255)
                                        aaCTTCAAGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGtata                                                                                                      >  3:243786/1‑141 (MQ=255)
                                        aaCTTCAAGCAATGATAACTGAATTACCACAACAGCGATTGG‑TTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTCTACCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTatat                                                                                                     >  1:18934/1‑141 (MQ=255)
                                         aCTTCAAGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTatat                                                                                                     >  4:190087/1‑141 (MQ=255)
                                             cAAGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTaa                                                                                                 >  2:720479/1‑141 (MQ=255)
                                             cAAGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTaa                                                                                                 <  1:37042/141‑1 (MQ=255)
                                              aaGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTaaa                                                                                                >  2:29396/1‑141 (MQ=255)
                                                gCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAat                                                                                              >  4:286076/1‑141 (MQ=255)
                                                  aaTGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAatat                                                                                            >  3:301015/1‑141 (MQ=255)
                                                    tGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAg                                                                                          <  1:145736/141‑1 (MQ=255)
                                                    tGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAg                                                                                          >  2:968674/1‑141 (MQ=255)
                                                    tGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTCGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGCAAGTTAATGGTGCTTGGTATATTTAAACtataa                                                                                          >  4:263416/1‑140 (MQ=255)
                                                     gATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGa                                                                                         <  1:137233/141‑1 (MQ=255)
                                                      aTAACTGAATTACCACAACAGCGATTGGATTTACCGAGTGATGTTGCTCATCCAGGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGaa                                                                                        >  2:311822/1‑141 (MQ=255)
                                                       tAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAAc                                                                                       >  1:45460/1‑141 (MQ=255)
                                                       tAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAAc                                                                                       >  1:137871/1‑141 (MQ=255)
                                                       tAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAAc                                                                                       <  3:426602/141‑1 (MQ=255)
                                                         aCTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACCTGCGCCAATTTATATCAACCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTa                                                                                     <  1:917988/141‑1 (MQ=255)
                                                             aaTTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTAtttt                                                                                 <  2:187773/141‑1 (MQ=255)
                                                                   cacaACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAg                                                                           >  2:666515/1‑141 (MQ=255)
                                                                     caacaGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTACAATATAGAACTATTATTATAAGCa                                                                         >  4:191595/1‑141 (MQ=255)
                                                                     caacaGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCa                                                                         <  1:2602/141‑1 (MQ=255)
                                                                      aacaGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCaa                                                                        >  3:246732/1‑141 (MQ=255)
                                                                      aacaGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCaa                                                                        <  3:222125/141‑1 (MQ=255)
                                                                        caGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAAt                                                                      >  4:399042/1‑141 (MQ=255)
                                                                              ttGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGTAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAGCTATTTTTATTAGCAAATGttttt                                                                <  2:914314/141‑1 (MQ=255)
                                                                              ttGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGttttt                                                                <  1:479477/141‑1 (MQ=255)
                                                                              ttGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGttttt                                                                <  2:356524/141‑1 (MQ=255)
                                                                               tGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTa                                                               <  1:567712/141‑1 (MQ=255)
                                                                                ggTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCTCCCACATGCTAGCCGTGTTCCAGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTac                                                              <  1:1029169/141‑2 (MQ=255)
                                                                                  ttttACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACTCAAATGCTAGAAGCGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTAtga                                                            <  2:575272/141‑1 (MQ=255)
                                                                                     tACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAGATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTAtgattg                                                         <  2:107224/141‑1 (MQ=255)
                                                                                     tACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTAtgattg                                                         <  1:637513/141‑1 (MQ=255)
                                                                                      aCCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTAtgattga                                                        <  3:63861/141‑1 (MQ=255)
                                                                                       ccGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTAtgattgat                                                       >  2:89544/1‑141 (MQ=255)
                                                                                        cGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACTCAAATGCTAGAAGCGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTATGATTGATg                                                      <  2:805180/141‑1 (MQ=255)
                                                                                        cGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTATGATTGATg                                                      <  2:817969/141‑1 (MQ=255)
                                                                                           gTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTATGATTGATGTta                                                   >  2:678695/1‑141 (MQ=255)
                                                                                              aGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTATGATTGATGTTataa                                                >  1:980572/1‑141 (MQ=255)
                                                                                               ggTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTATGATTGATGTTataat                                               >  2:625311/1‑141 (MQ=255)
                                                                                                gTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAACTGTTTTTATGATTGATGTTataata                                              >  3:267842/1‑141 (MQ=255)
                                                                                                gTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTATGATTGATGTTataata                                              <  2:744705/141‑1 (MQ=255)
                                                                                                  tGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTATGATTGATGTTATAATATg                                            >  2:971732/1‑141 (MQ=255)
                                                                                                  tGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGATAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAAAGTTTTTTTGATTGATGTTATAatatt                                            >  2:507928/1‑140 (MQ=255)
                                                                                                   gCTCATACATGCGCCTATTTATATCACTCAAATGCTAGAAGCGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTATGATTGATGTTATAATATGa                                           >  1:980406/1‑141 (MQ=255)
                                                                                                   gCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTAtgattgat                                                       >  2:225080/1‑129 (MQ=255)
                                                                                                   gCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTAtgattgat                                                       <  1:225080/129‑1 (MQ=255)
                                                                                                   gCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTATGATTGATGTTATAATATGa                                           >  4:47936/1‑141 (MQ=255)
                                                                                                   gCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTATGATTGATGTTATAATATGa                                           <  4:224973/141‑1 (MQ=255)
                                                                                                          cATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTATGATTGATGTTATAATATGAATATTAc                                    >  1:556468/1‑141 (MQ=255)
                                                                                                          cATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTATGATTGATGTTATAATATGAATATTAc                                    >  2:239854/1‑141 (MQ=255)
                                                                                                              cgcCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTATGATTGATGTTATAATATGAATATTACAATg                                >  1:675943/1‑141 (MQ=255)
                                                                                                              cgcCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTATGATTGATGTTATAATATGAATATTACAATg                                >  4:457111/1‑141 (MQ=255)
                                                                                                              cgcCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTATGATTGATGTTATAATATGAATATTACAATg                                >  2:1031563/1‑141 (MQ=255)
                                                                                                              cgcCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTATGATTGATGTTATAATATGAATATTACAATg                                >  4:128461/1‑141 (MQ=255)
                                                                                                              cgcCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTATGATTGATGTTATAATATGAATATTACAATg                                >  2:32014/1‑141 (MQ=255)
                                                                                                                ccTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTATGATTGATGTTATAATATGAATATTACAATGaa                              >  2:300217/1‑141 (MQ=255)
                                                                                                                ccTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTATGATTGATGTTATAATATGAATATTACAATGaa                              >  1:801642/1‑141 (MQ=255)
                                                                                                                ccTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTATGATTGATGTTATAATATGAATATTACAATGaa                              >  2:688183/1‑141 (MQ=255)
                                                                                                                 cTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTATGATTGATGTTATAATATGAATATTACAATGAAt                             <  2:550882/141‑1 (MQ=255)
                                                                                                                 cTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTATGATTGATGTTATAATATGAATATTACAATGAAt                             <  1:952735/141‑1 (MQ=255)
                                                                                                                   aTTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAGCTATTTTTATGAGCAAATGTTTTTATGATTGATGTTATAATATGAATATTACAATGAATCa                           <  2:325213/141‑1 (MQ=255)
                                                                                                                        tatCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTATGATTGATGTTATAATATGAATATTACAATGAATCATAAtt                      >  2:199517/1‑141 (MQ=255)
                                                                                                                          tCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTATGATTGATGTTATAATATGAATATTACAATGAATCATAATTTg                    <  3:95966/141‑1 (MQ=255)
                                                                                                                          tCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTATGATTGATGTTATAATATGAATATTACAATGAATCATAATTTg                    >  2:866068/1‑141 (MQ=255)
                                                                                                                          tCACCCAAACGCTAGAAGTGTTACTGGAACTATACATAAAGTAACTGGTGCGTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGCTTTTATGATTGATGTTCTAATATGAATATTACGATGAATCATaactcg                    >  2:805067/1‑137 (MQ=255)
                                                                                                                               cAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTATGATTGATGTTATAATATGAATATTACAATGAATCATAATTTGttaat               >  3:174978/1‑141 (MQ=255)
                                                                                                                                   tGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTATGATTGATGTTATAATATGAATATTACAATGAATCATAATTTGTTAATTaaa           >  1:921965/1‑141 (MQ=255)
                                                                                                                                   tGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTATGATTGATGTTATAATATGAATATTACAATGAATCATAATTTGTTAATTaaa           <  1:205156/141‑1 (MQ=255)
                                                                                                                                       aGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTATGATTGATGTTATAATATGAATATTACAATGAATCATAATTTGTTAATTAAACAAg       >  1:878141/1‑141 (MQ=255)
                                                                                                                                       aGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTATGATTGATGTTATAATATGAATATTACAATGAATCATAATTTGTTAATTAAACAAg       >  2:692959/1‑141 (MQ=255)
                                                                                                                                        gAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTATGATTGATGTTATAATATGAATATTACAATGAATCATAATTTGTTAATTAAACAAgg      <  1:1055261/141‑1 (MQ=255)
                                                                                                                                         aaGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTATGATTGATGTTATAATATGAATATTACAATGAATCATAATTTGTTAATTAAACAAggg     >  4:28326/1‑141 (MQ=255)
                                                                                                                                         aaGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTATGATTGATGTTATAATATGAATATTACAATGAATCATAATTTGTTAATTAAACAAggg     >  1:372748/1‑141 (MQ=255)
                                                                                                                                            tgtTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTATGATTGATGTTATAATATGAATATTACAATGAATCATAATTTGTTAATTAAACAAGGGGTg  <  2:532523/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                            
TTTGTAGCCGGTAATATGGCAAGTGAGTGGCAAGAAGATGAACTTCAAGCAATGATAACTGAATTACCACAACAGCGATTGGTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGTGTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAAAATATAGAACTATTTTTATGAGCAAATGTTTTTATGATTGATGTTATAATATGAATATTACAATGAATCATAATTTGTTAATTAAACAAGGGGTG  >  USA300TCH1516_ALE/1306457‑1306737

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑