Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 1,346,192 C→T 5.0% intergenic (+415/+134) USA300HOU_RS06685 → / ← ltaE hypothetical protein/Low specificity L‑threonine aldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,346,1920CT 5.0% 361.5 / 2.7 113intergenic (+415/+134)USA300HOU_RS06685/ltaEhypothetical protein/Low specificity L‑threonine aldolase
Reads supporting (aligned to +/- strand):  ref base C (44/62);  new base T (3/4);  total (47/66)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 118 total aligned reads displayed.

CGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATGAGCAGTTTCATAT‑‑ATAAATAAACACATCTACTCAACTGATCTTAATTAATTAATAAATGATTTACCAGATGACATATAACAGCATTATTAGATT  >  USA300TCH1516_ALE/1346052‑1346332
                                                                                                                                            |                                                                                                                                              
cGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTAc                                                                                                                                                <  4:383155/141‑1 (MQ=255)
cGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTAc                                                                                                                                                <  1:371647/141‑1 (MQ=255)
cGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTAc                                                                                                                                                <  2:438336/141‑1 (MQ=255)
cGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTCCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTAc                                                                                                                                                <  1:695818/141‑1 (MQ=255)
  aTTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCt                                                                                                                                              <  3:273495/141‑1 (MQ=255)
  aTTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATCAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCt                                                                                                                                              >  4:315769/1‑141 (MQ=255)
     gTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGt                                                                                                                                           <  2:1042032/141‑1 (MQ=255)
        aaTACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGtttt                                                                                                                                        <  2:599781/141‑1 (MQ=255)
        aaTACATCACTTTCATAAAATAACTATAACCATCATAATACTTCTCAAATCTCGTCAAAATAAAGAAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACAAAATAATATGTAGTATCTTGtttt                                                                                                                                        >  3:121214/1‑141 (MQ=255)
           aCATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGa                                                                                                                                     >  1:4469/1‑141 (MQ=255)
            cATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGaa                                                                                                                                    <  4:56009/141‑1 (MQ=255)
                  tttCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGt                                                                                                                              <  3:336960/141‑1 (MQ=255)
                    tCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTaa                                                                                                                            >  2:442035/1‑141 (MQ=255)
                    tCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTaa                                                                                                                            >  2:958232/1‑141 (MQ=255)
                    tCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAAGACGGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTaa                                                                                                                            <  1:373613/141‑1 (MQ=255)
                     cATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTATCAGTTTTTGGAATAATGTAAt                                                                                                                           >  1:826313/1‑141 (MQ=255)
                     cATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAAt                                                                                                                           <  2:720922/141‑1 (MQ=255)
                      aTAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAAtt                                                                                                                          >  1:29468/1‑141 (MQ=255)
                      aTAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAAtt                                                                                                                          >  1:395875/1‑141 (MQ=255)
                           aTAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTtata                                                                                                                     >  3:396697/1‑141 (MQ=255)
                           aTAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTtata                                                                                                                     >  1:731298/1‑141 (MQ=255)
                            tAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTatat                                                                                                                    <  1:1006269/141‑1 (MQ=255)
                            tAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTatat                                                                                                                    <  1:665753/141‑1 (MQ=255)
                            tAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTatat                                                                                                                    <  1:547845/141‑1 (MQ=255)
                             aaCTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATatc                                                                                                                   <  2:1067895/141‑1 (MQ=255)
                               cTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATatcat                                                                                                                 >  1:868818/1‑141 (MQ=255)
                                 aCAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGAAAATACAGCACACATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATAtcatca                                                                                                               <  2:933889/141‑1 (MQ=255)
                                      catcatAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAGATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGc                                                                                                          <  2:952838/141‑1 (MQ=255)
                                      catcatAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGc                                                                                                          <  1:802886/141‑1 (MQ=255)
                                        tcatAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTa                                                                                                        >  1:197275/1‑141 (MQ=255)
                                         catAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTAc                                                                                                       <  2:552808/141‑1 (MQ=255)
                                           taataCTTCTCAAATCTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTATCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTTCaa                                                                                                     <  1:224328/141‑1 (MQ=255)
                                              taCTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATg                                                                                                  <  2:189177/141‑1 (MQ=255)
                                               aCTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATGa                                                                                                 <  1:508426/141‑1 (MQ=255)
                                               aCTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATGa                                                                                                 <  4:400922/141‑1 (MQ=255)
                                                 ttCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATGAGc                                                                                               <  2:860160/141‑1 (MQ=255)
                                                 tactcAAATGTCGTGAAATTAGAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCTATAATCTGTAGTACCGTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATGAGc                                                                                               <  2:781022/139‑1 (MQ=255)
                                                  tctcAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATGAGCa                                                                                              >  3:54551/1‑141 (MQ=255)
                                                   ctcAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATGAGCAg                                                                                             <  4:261149/141‑1 (MQ=255)
                                                     cAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATGAGCAGtt                                                                                           <  2:618811/141‑1 (MQ=255)
                                                     aaaaTGTAGTCAAAGTAAAGTATAAACAGCACAAATTAACATTTTTAAATATATAATATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACTGCTACATATGAGCAGtt                                                                                           <  4:225402/140‑1 (MQ=255)
                                                      aaaTGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATGAGCAGttt                                                                                          <  3:166742/141‑1 (MQ=255)
                                                       aaTGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATGAGCAGTTTc                                                                                         >  1:368454/1‑141 (MQ=255)
                                                        aTGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACTTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATGAGCAGTTTCa                                                                                        <  2:945615/141‑1 (MQ=255)
                                                          gtcgtcAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATGAGCAGTTTCata                                                                                      >  2:235098/1‑141 (MQ=255)
                                                           tcgtcAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATGAGCAGTTTCatat                                                                                     <  1:554924/141‑1 (MQ=255)
                                                              tcAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATGAGCAGTTTCAtat‑‑ata                                                                                <  3:345807/141‑1 (MQ=255)
                                                               cAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATGAGCAGTTTCATAT‑‑ataa                                                                               <  2:220279/141‑1 (MQ=255)
                                                                 aaTTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATGAGCAGTTTCATAT‑‑ataaat                                                                             >  2:27548/1‑141 (MQ=255)
                                                                  aTTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTATCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTTCAAATGAGTAGTTTCatat‑‑aaatcaa                                                                            <  1:339678/141‑7 (MQ=255)
                                                                   ttAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTATCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTTCAAATGAGTAGTTTCatat‑‑aaatcaat                                                                           <  2:1043040/141‑8 (MQ=255)
                                                                       aGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATGAGCAGTTTCATAT‑‑ATAAATAAacac                                                                       >  2:141312/1‑141 (MQ=255)
                                                                        gTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATGAGCAGTCTCATAT‑‑ATAAATAAAcaca                                                                      <  1:924454/141‑1 (MQ=255)
                                                                        gTAAAGACAGCACAAATTAACATTGTTAAATATATAAAATAGAACAAGAAACGCAATAATCTGTATTACCTTGTTTTGGAATAATGGAATTTTATATTATCAACGTCTACAATTGATCAGGTTCAGAT‑‑ataaataatctct                                                                      >  4:340711/1‑136 (MQ=255)
                                                                               cAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATGAGCAGTTTCATAT‑‑ATAAATAAACACATCTACTc                                                               <  4:255480/141‑1 (MQ=255)
                                                                                 gCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATGAGCAGTTTCATAT‑‑ATAAATAAACACATCTACTCaa                                                             <  1:706152/141‑1 (MQ=255)
                                                                                 acacaTAATATCATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATGAGCAGTTTCATAT‑‑ATAAATAAACACATCTACTCaa                                                             <  2:39508/140‑1 (MQ=255)
                                                                                  cacaAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATGAGCAGTTTCATAT‑‑ATAAATAAACACATCTACTCAAc                                                            <  2:680379/141‑1 (MQ=255)
                                                                                    caAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTATCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTTCAAATGAGTAGTTTCATATAAATCAATAAACACAACTACTCAAc                                                            >  2:372335/1‑141 (MQ=255)
                                                                                    caAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATGAGCAGTTTCATAT‑‑ATAAATAAACACATCTACTCAACTg                                                          >  3:453656/1‑141 (MQ=255)
                                                                                      aaTTAACATTTTTAAATATATAAAATAAAACAAGATACTTAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATGAGCAGTTTCATAT‑‑ATAAATAAACACATCTACTCAACTGAt                                                        <  4:118646/141‑1 (MQ=255)
                                                                                      aaTTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTATCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTTCAAATGAGTAGTTTCATATAAATCAATAAACACAACTACTCAACTg                                                          <  1:671660/141‑1 (MQ=255)
                                                                                       aTTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATGAGCAGTTTCATAT‑‑ATAAATAAACACATCTACTCAACTGATc                                                       >  2:50223/1‑141 (MQ=255)
                                                                                       aTTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATGAGCAGTTTCATAT‑‑ATAAATAAACACATCTACTCAACTGATc                                                       >  2:366573/1‑141 (MQ=255)
                                                                                        ttAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATGAGCAGTTTCATAT‑‑ATAAATAAACACATCTACTCAACTGATCt                                                      <  2:297008/141‑1 (MQ=255)
                                                                                        atAACATTTTTAAATATATATAATAAAACAAGTTTCTCATTAATCTGTAGTACCTTGTTTTGGAATAATTTAATTTTATATCATCAACGGCTACAAATGATCAGTTTCATAT‑‑ATAAATAAACACATCTTCTCAACTGATCt                                                      <  2:644949/140‑1 (MQ=255)
                                                                                            cATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATGAGCAGTTTCATAT‑‑ATAAATAAACACATCTACTCAACTGATCttaat                                                  <  2:84706/141‑1 (MQ=255)
                                                                                            cATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATGAGCAGTTTCATAT‑‑ATAAATAAACACATCTACTCAACTGATCttaat                                                  <  2:559928/141‑1 (MQ=255)
                                                                                             aTTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATGAGCAGTTTCATAT‑‑ATAAATAAACACATCTACTCAACTGATCttaatt                                                 <  1:198491/141‑1 (MQ=255)
                                                                                               ttttAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATGAGCAGTTTCATAT‑‑ATAAATAAACACATCTACTCAACTGATCttaattaa                                               <  2:1062877/141‑1 (MQ=255)
                                                                                                tttAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATGAGCAGTTTCATAT‑‑ATAAATAAACACATCTACTCAACTGATCttaattaat                                              >  4:168107/1‑141 (MQ=255)
                                                                                                      tatataAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATGAGCAGTTTCATAT‑‑ATAAATAAACACATCTACTCAACTGATCTTAATTAATtaataa                                        <  4:186619/141‑1 (MQ=255)
                                                                                                      tatataAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATGAGCAGTTTCATAT‑‑ATAAATAAACACATCTACTCAACTGATCTTAATTAATtaataa                                        >  2:615732/1‑141 (MQ=255)
                                                                                                      tatataAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATGAGCAGTTTCATAT‑‑ATAAATAAACACATCTACTCAACTGATCTTAATTAATtaataa                                        >  1:911700/1‑141 (MQ=255)
                                                                                                        tataAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATGAGCAGTTTCATAT‑‑ATAAATAAACACATCTACTCAACTGATCTTAATTAATTAATAAAt                                      >  3:197949/1‑141 (MQ=255)
                                                                                                         ataaaataaaACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATGAGCAGTTTCCTAT‑‑ATAAATAAACACATCTACTCAACTGATCTTAATTAATTAATAAATg                                     >  1:1084600/1‑141 (MQ=255)
                                                                                                          taaaataaaaCAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATGAGCAGTTTCATAT‑‑ATAAATAAACACATCTACTCAACTGATCTTAATTAATTAATAAATGa                                    >  1:1002384/1‑141 (MQ=255)
                                                                                                           aaaataaaaCAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATGAGCAGTTTCATAT‑‑ATAAATAAACACATCTACTCAACTGATCTTAATTAATTAATAAATGAt                                   <  1:94982/141‑1 (MQ=255)
                                                                                                            aaataaaaCAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATGAGCAGTTTCATAT‑‑ATAAATAAACACATCTACTCAACTGATCTTAATTAATTAATAAATGAtt                                  <  1:273656/141‑1 (MQ=255)
                                                                                                                aaaaCAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATGAGCAGTTTCATAT‑‑ATAAATAAAGGAATCTACTCAACTGATCTTAATTAATTAATAAATGATTTAcc                              >  1:284538/1‑141 (MQ=255)
                                                                                                                 aaaCAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATGAGCAGTTTCATAT‑‑ATAAATAAACACATCTACTCAACTGATCTTAATTAATTAATAAATGATTTACCa                             >  2:851786/1‑141 (MQ=255)
                                                                                                                  aaCAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATGAGCAGTTTCATAT‑‑ATAAATAAACACATCTACTCAACTGATCTTAATTAATTAATAAATGATTTACCAg                            <  1:433767/141‑1 (MQ=255)
                                                                                                                  aaCAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATGAGCAGTTTCATAT‑‑ATAAATAAACACATCTACTCAACTGATCTTAATTAATTAATAAATGATTTACCAg                            >  4:286595/1‑141 (MQ=255)
                                                                                                                  aaCAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATCAGCAGTTTCATAT‑‑ATAAATAAACACATATACTCAACTGATCTTAATTAATTAATAAATGATTTAACAg                            >  4:286664/1‑141 (MQ=255)
                                                                                                                      aGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATGAGCAGTTTCATAT‑‑ATAAATAAACACATCTACTCAACTGATCTTAATTAATTAATAAATGATTTACCAGATGa                        <  3:208040/141‑1 (MQ=255)
                                                                                                                      aGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATGAGCAGTTTCATAT‑‑ATAAAAAAACACATCTACTCAACTGATCTTAATTAATTAATAAATGATTTACCAGATGa                        <  2:666081/141‑1 (MQ=255)
                                                                                                                      aGATACCCCACAACCTGTAGTACCTTGTTTTGGAATAATGTAATTTTCTACCACCAACGATTTATTACGTAATGTCTCAAAT‑‑CTAAATAAACACATCTACTCAACTGATCTTAATTAATTAATAAATGATTTACCAGATGa                        <  3:340711/141‑1 (MQ=255)
                                                                                                                           cTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATGAGCAGTTTCATAT‑‑ATAAATAAACACATCTACTCAACTGATCTTAATTAATTAATAAATGATTTACCAGATGACatat                   >  1:272860/1‑141 (MQ=255)
                                                                                                                              aataatCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATGAGCAGTTTCATAT‑‑ATAAATAAACACATCTACTCAACTGATCTTAATTAATTAATAAATGATTTACCAGATGACATATAAc                <  2:265815/141‑1 (MQ=255)
                                                                                                                                 aatCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATGAGCAGTTTCATAT‑‑ATAAATAAACACATCTACTCAACTGATCTTAATTAATTAATAAATGATTTACTAGATGACATATAACAGc             >  2:545458/1‑141 (MQ=255)
                                                                                                                                    cTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATGAGCAGTTTCATAT‑‑ATAAATAAACACATCTACTCAACTGATCTTAATTAATTAATAAATGATTTACCAGATGACATATAACAGCatt          >  2:717480/1‑141 (MQ=255)
                                                                                                                                    cTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATGAGCAGTTTCATAT‑‑ATAAATAAACACATCTACTCAACTGATCTTAATTAATTAATAAATGATTTACCAGATGACATATAACAGCatt          >  4:53796/1‑141 (MQ=255)
                                                                                                                                    cTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATGAGCAGTTTCATAT‑‑ATAAATAAACACATCTACTCAACTGATCTTAATTAATTAATAAATGATTTACCAGATGACATATAACAGCatt          >  3:29836/1‑141 (MQ=255)
                                                                                                                                     tGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATGAGCAGTTTCATAT‑‑ATAAATAAACACATCTACTCAACTGATCTTAATTAATTAATAAATGATTTACCAGATGACATATAACAGCatta         >  2:971638/1‑141 (MQ=255)
                                                                                                                                      gtagtaCCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATGAGCAGTTTCATAT‑‑ATAAATAAACACATCTACTCAACTGATCTTAATTAATTAATAAATGATTTACCAGATGACATATAACAGCattat        >  1:277445/1‑141 (MQ=255)
                                                                                                                                       tagtaCCTTGTTTTGGAATAATGTAATTTTATATGATCAACGGCTACAAATGAGCAGTTACAAAT‑‑ATAAATAAACACATCAACTCAAATGATCTTAATTAATTAATAAATGATCTACTAGATGACATATAAGAGCgttatt       >  4:284968/1‑141 (MQ=255)
                                                                                                                                         gtaCCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATGAGCAGTTTCATAT‑‑ATAAATAAACACATCTACTCAACTGATCTTAATTAATTAATAAATGATTTACCAGATGACATATAACAGCATTATTAg     >  4:189225/1‑141 (MQ=255)
                                                                                                                                          taCCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATGAGCAGTTTCATAT‑‑ATAAATAAACACATCTACTCAACTGATCTTAATTAATTAATAAATGATTTACCAGATGACATATAACAGCATTATTAGa    <  1:419995/141‑1 (MQ=255)
                                                                                                                                           aCCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATGAGCAGTTTCATAT‑‑ATAAATAAACACATCTACTCAACTGATCTTAATTAATTAATAAATGATTTACCAGATGACATATAACAGCATTATTAGAt   <  1:320232/141‑1 (MQ=255)
                                                                                                                                            ccTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATGAGCAGTTTCATAT‑‑ATAAATAAACACATCTACTCAACTGATCTTAATTAATTAATAAATGATTTACCAGATGACATATAACAGCATTATTAGAtt  >  2:989486/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                              
CGATTGTTAATACATTACTTTCATAAAATAACTACAACCATCATAATACTTCTCAAATGTCGTCAAATTAAAGTAAATACAGCACAAATTAACATTTTTAAATATATAAAATAAAACAAGATACTCAATAATCTGTAGTACCTTGTTTTGGAATAATGTAATTTTATATCATCAACGGCTACAAATGAGCAGTTTCATAT‑‑ATAAATAAACACATCTACTCAACTGATCTTAATTAATTAATAAATGATTTACCAGATGACATATAACAGCATTATTAGATT  >  USA300TCH1516_ALE/1346052‑1346332

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: