BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	1,354,058	T→G	5.5%	T249T (ACA→ACC)	USA300HOU_RS06735 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,354,058	0	T	G	5.5%	414.9 / 12.2	138	T249T (ACA→ACC)	USA300HOU_RS06735	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (73/57); new base G (6/2); total (79/59)
Fisher's exact test for biased strand distribution p-value = 4.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 138 total aligned reads displayed.

GAGCGTAATGGATTATTATGCCTTTGATTTTACTTGAATATAGCTGTGATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACACCGGTACTTTGGACTTTAAAGTTAAAGTTTTGTTCAATATGATCCTTTAATGCTTTCGCATTATTTG  >  USA300TCH1516_ALE/1353921‑1354197

gAGCGTAATGTATCATTATGCCTTTGATTTTACTTGAATATAGCTGTGATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTa                                                                                                                                          <  4:380300/141‑1 (MQ=255)

  gCGTAATGGATTATTATGCCTTTGATTTTACTTGAATATAGCTGTGATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATc                                                                                                                                        <  2:453621/141‑1 (MQ=255)

   cGTAATGGATTATTATGCCTTTGATTTTACTTGAATATAGCTGTGATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCa                                                                                                                                       >  2:329828/1‑141 (MQ=255)

      aaTGGATTATTATGCCTTTGATTTTACTTGAATATAGCTGTGATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACa                                                                                                                                    <  1:749667/141‑1 (MQ=255)

      aaTGGATTATTATGCCTTTGATTTTACTTGAATATAGCTGTGATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACa                                                                                                                                    <  3:415012/141‑1 (MQ=255)

         ggATTATTATGCCTTTGATTTTACTTGAATATAGCTGTGATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACatat                                                                                                                                 >  1:710376/1‑141 (MQ=255)

         ggATTATTATGCCTTTGATTTTACTTGAATATAGCTGTGATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACatat                                                                                                                                 >  1:769587/1‑141 (MQ=255)

           attattATGCCTTTGATTTTACTTGAATATAGCTGTGATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATAttt                                                                                                                               <  2:504823/141‑1 (MQ=255)

              attatGCCTTTGATTTTACTTGAATATAGCTGTGATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTAtc                                                                                                                            <  3:326317/141‑1 (MQ=255)

              attatGCCTTTGATTTTACTTGAATATAGCTGCGATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTAtc                                                                                                                            >  2:854197/1‑141 (MQ=255)

                tatGCCTTTGATTTTACTTGAATATAGCTGTGATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTAtctc                                                                                                                          >  2:579879/1‑141 (MQ=255)

                  tGCCTTTGATTTTACTTGAATATAGCTGTGATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCtctt                                                                                                                        >  2:527197/1‑141 (MQ=255)

                  tGCCTTTGATTTTACTTGAATATAGCTGTGATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCtctt                                                                                                                        >  4:119357/1‑141 (MQ=255)

                       ttGATTTTACTTGAATATAGCTGTGATTTTATCAACCTGTTTTTTTTTTTAAGGGTGTTTTGATAGCTCTATGGCTTCTCTAATAATACGTTGTAAAGAAGATTTTGTTTTAACTGTATCAACATATTTATTtctttcttt                                                                                                                   >  2:243909/1‑140 (MQ=255)

                        tGATTTTACTTGAATATAGCTGTGATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCa                                                                                                                  <  2:185008/141‑1 (MQ=255)

                         gagtttACTAGAATATAGCTGTGAGTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAg                                                                                                                 <  2:311351/138‑1 (MQ=255)

                         gATTTTACTTGAATATAGCTGTGATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAg                                                                                                                 <  2:19966/141‑1 (MQ=255)

                          aTTTTACTTGAATATAGCTGTGATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGa                                                                                                                <  1:1020904/141‑1 (MQ=255)

                             ttACTTGAATATAGCTGTGATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAAcc                                                                                                             <  1:960078/141‑1 (MQ=255)

                              tACTTGAATATAGCTGTGATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCt                                                                                                            <  1:927287/141‑1 (MQ=255)

                              tACTTGAATATAGCTGTGATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCt                                                                                                            <  2:904129/141‑1 (MQ=255)

                               aCTTGAATATAGCTGTGATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAATGAAGATTTTGTTGAAACGGTATCAACATATTTATCTCTTTCTTCAGAACCtt                                                                                                           >  1:570734/1‑141 (MQ=255)

                                cTTGAATATAGCTGTGATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTg                                                                                                          >  1:730577/1‑141 (MQ=255)

                                   gAATATAGCTGTGATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAAt                                                                                                       <  1:1023827/141‑1 (MQ=255)

                                   gAATATAGCTGTGATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAAt                                                                                                       >  1:316231/1‑141 (MQ=255)

                                    aaTATAGCTGTGATTTGATCAATCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGCTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATg                                                                                                      <  3:379175/141‑1 (MQ=255)

                                    aaTATAGCTGTGATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATg                                                                                                      <  1:806503/141‑1 (MQ=255)

                                      tataGCTGTGATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGat                                                                                                    <  2:62893/141‑1 (MQ=255)

                                          gCTGTGATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTa                                                                                                >  1:678647/1‑141 (MQ=255)

                                          gCTGTGATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTa                                                                                                >  1:738405/1‑141 (MQ=255)

                                          gCTGTGATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTa                                                                                                >  1:840579/1‑141 (MQ=255)

                                          gCTGTGATTTGATCAACCTGTTATTTGTTAAAAGGGGGTTTAGATAGCTCTATGGCTCCCCTAATAAGACGCCGGAATGAAAATTTTGTTGCAACGTAACCAACATAGTTATCTCTTCTCTCAGAACCTAGACTGATATTa                                                                                                >  4:197082/1‑141 (MQ=255)

                                           cTGTTAATTTATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTaa                                                                                               <  4:242952/141‑1 (MQ=255)

                                           cTGTGATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTaa                                                                                               <  4:242965/141‑1 (MQ=255)

                                            tgtgATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAAc                                                                                              >  2:658203/1‑141 (MQ=255)

                                              tgATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTg                                                                                            <  1:44163/141‑1 (MQ=255)

                                                aTTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTa                                                                                          >  3:341515/1‑141 (MQ=255)

                                                  ttGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTAcc                                                                                        >  1:684242/1‑141 (MQ=255)

                                                  ttGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTAcc                                                                                        <  1:471591/141‑1 (MQ=255)

                                                   tGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAATACATTGATCTCCTTGTTCTGCACCGTGAAAGATTTTAACAGTAccc                                                                                       >  1:218152/1‑140 (MQ=255)

                                                    gATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCAt                                                                                      >  1:184410/1‑141 (MQ=255)

                                                      tCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCa                                                                                    <  4:29204/141‑1 (MQ=255)

                                                       cAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCAc                                                                                   >  3:116567/1‑141 (MQ=255)

                                                        aaCCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACt                                                                                  <  2:954802/141‑1 (MQ=255)

                                                          ccTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTaa                                                                                <  2:360730/141‑1 (MQ=255)

                                                           cTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTATATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTaaa                                                                               <  2:1009347/141‑1 (MQ=255)

                                                             gTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGt                                                                             >  4:54101/1‑141 (MQ=255)

                                                              ttCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTa                                                                            <  2:659076/141‑1 (MQ=255)

                                                               tCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTaa                                                                           >  1:595478/1‑141 (MQ=255)

                                                                  ttatttaAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATctct                                                                                                                         >  1:634130/1‑92 (MQ=255)

                                                                    atttaAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAac                                                                      >  1:987746/1‑141 (MQ=255)

                                                                     tttaAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAaca                                                                     >  1:639881/1‑141 (MQ=255)

                                                                      ttaAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAacac                                                                    >  4:37440/1‑141 (MQ=255)

                                                                       taAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACAcc                                                                   <  1:895686/141‑1 (MQ=255)

                                                                       taAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACAcc                                                                   >  2:846021/1‑141 (MQ=255)

                                                                         aaGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACACAgg                                                                 >  1:223099/1‑141 (MQ=255)

                                                                         aaGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGGCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACACCgg                                                                 >  2:663604/1‑141 (MQ=255)

                                                                          aGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACACCGGt                                                                >  1:438756/1‑141 (MQ=255)

                                                                          aGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACACCGGt                                                                >  2:797227/1‑141 (MQ=255)

                                                                          aGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACACCGGt                                                                >  2:940550/1‑141 (MQ=255)

                                                                            ggTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAATGAAGATTTTGTTGAAACGGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACACCGGTAc                                                              >  1:54963/1‑141 (MQ=255)

                                                                              tgtTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACACCGGTACtt                                                            >  2:379795/1‑141 (MQ=255)

                                                                              tgtTTAGATAGCTCTANGGCTTCGCTAATNATACGTCGTAACGAAGATTTTGTTGAAACTGTATCTACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACACCGGTACtt                                                            >  1:759365/1‑141 (MQ=255)

                                                                               gtTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACACCGGTACttt                                                           >  4:450335/1‑141 (MQ=255)

                                                                                tttAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACACCGGTACTTTg                                                          <  2:393991/141‑1 (MQ=255)

                                                                                tttAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACACCGGTACTTTg                                                          <  3:126208/141‑1 (MQ=255)

                                                                                tttAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACACCGGTACTTTg                                                          <  2:999376/141‑1 (MQ=255)

                                                                                 ttAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACACCGGTACTTTgg                                                         <  2:1054304/141‑1 (MQ=255)

                                                                                 ttAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACACCGGTACTTTgg                                                         >  2:1035445/1‑141 (MQ=255)

                                                                                     aTAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACACCGGTACTTTGGACtt                                                     >  2:984474/1‑141 (MQ=255)

                                                                                     aTAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACACCGGTACTTTGGACtt                                                     >  4:404642/1‑141 (MQ=255)

                                                                                             aTGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACACCGGTACTTTGGACTttaaagtta                                             <  1:985984/141‑1 (MQ=255)

                                                                                               ggCTTCGCTAATAATACGTCGTAATGAAGATTTTGTTGAAACGGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACACCGGTACTTTGGACTttaaagttaaa                                           >  1:163909/1‑141 (MQ=255)

                                                                                               ggCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACACCGGTACTTTGGACTttaaagttaaa                                           <  2:915953/141‑1 (MQ=255)

                                                                                                  ttCGCTAATAATACGTCGTAATGAAGATTTTGTTGAAACGGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACACCGGTACTTTGGACTTTaaagttaaagtt                                        <  3:117064/141‑1 (MQ=255)

                                                                                                  ttCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACACCGGTACTTTGGACTTTaaagttaaagtt                                        <  2:176439/141‑1 (MQ=255)

                                                                                                   tCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTTTACCATCACTAAAGTAAATAACACCGGTACTTTGGACTTTAAAGTTAAAGttt                                       <  1:550205/141‑1 (MQ=255)

                                                                                                   tCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACACCGGTACTTTGGACTTTAAAGTTAAAGttt                                       <  1:718907/141‑1 (MQ=255)

                                                                                                      cTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTCCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACACCGGTACTTTGGACTTTAAAGTTAAAGTTTTGt                                    >  1:891431/1‑141 (MQ=255)

                                                                                                        aataatACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACACCGGTACTTTGGACTTTAAAGTTAAAGTTTTGTTc                                  >  3:93478/1‑141 (MQ=255)

                                                                                                         ataataCGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGGACCATCACTAAAGTAAATAACACCGGTACTTTAGACTTTAAAGTTAAAGTTTTGTTCa                                 <  3:424139/141‑1 (MQ=255)

                                                                                                           aataCGTCGTAACGAAGATTTTGTTGAAGCTGTATCAACATATTTATCTCTTTCTTCAGAACCGTGAATGATATTAACTGTACCATCACTAAAGTAAATAACACCGGTACTTTGGACTTTAAAGTTAAATTTTTGTTCAat                               <  3:223316/141‑1 (MQ=255)

                                                                                                            ataCGTCTTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACACCGGTACTTTGGACTTTAAAGTTAAAGTTTTGTTCAata                              >  4:220935/1‑141 (MQ=255)

                                                                                                                ctcatAACGAAGATTTTGGTGACTCTTTAGAAACATATTTATCTCTTTCCTCAGTCCCTATCATTATATTCTTTGTCCCTTCACTAAAGTAAATAACGCTGGTACTTTGGACTTTAATGTGAAAGTTTTGTTCAATATGAt                          <  4:137497/137‑1 (MQ=255)

                                                                                                                      aCGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACACCGGTACTTTGGACTTTAAAGTTAAAGTTTTGTTCAATATGATCCTTTa                    >  1:931133/1‑141 (MQ=255)

                                                                                                                      aCGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACACCGGTACTTTGGACTTTAAAGTTAAAGTTTTGTTCAATATGATCCTTTa                    >  1:486142/1‑141 (MQ=255)

                                                                                                                       ccaaGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTAAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACACCGGTACTTTGGACTTTAAAGTTAAAGTTTTGTTCAATATGATCCTTTaa                   <  4:406377/139‑1 (MQ=255)

                                                                                                                          aGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACACCGGTACTTTGGACTTTAAAGTTAAAGTTTTGTTCAATATGATCCTTTAATGc                >  2:95382/1‑141 (MQ=255)

                                                                                                                          aGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTAACATCACTAAAGTCTATAACACCGGTATTTTGGAGTTTACCGTTAACATCTAGTTCATTCTGATCCTTTAATGc                >  3:89361/1‑141 (MQ=255)

                                                                                                                           gATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACACCGGTACTTTGGACTTTAAAGTTAAAGTTTTGTTCAATATGATCCTTTAATGCt               <  3:350623/141‑1 (MQ=255)

                                                                                                                               ttgttgAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGCAAATAACACCGGTACTTTGGACTTTAAAGTTAAAGTTTTGTTCAATATGATCCTTTAATGCTTTCg           >  1:795331/1‑141 (MQ=255)

                                                                                                                                tgttgAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACACCGGTACTTTGGACTTTAAAGTTAAAGTTTTGTTCAATATGATCCTTTAATGCTTTCGc          <  1:399895/141‑1 (MQ=255)

                                                                                                                                 gttgAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACACCGGTACTTTGGACTTTAAAGTTAAAGTTTTGTTCAATATGATCCTTTAATGCTTTCGCa         <  2:592257/141‑1 (MQ=255)

                                                                                                                                 gttgAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACACCGGTACTTTGGACTTTAAAGTTAAAGTTTTGTTCAATATGATCCTTTAATGCTTTCGCa         >  1:401501/1‑141 (MQ=255)

                                                                                                                                 gttgAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTAAATGATATTAACTGTACCATCACTAAAGTAAATAACACCGGTACTTTGGACTTTAAAGTTAAAGTTTTGTTCAATATGATCCTTTAATGCTTTCGCa         >  2:362364/1‑141 (MQ=255)

                                                                                                                                  ttgAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACACCGGTACTTTGGACTTTAAAGTTAAAGTTTTGTTCAATATGATCCTTTAATGCTTTCGCat        <  1:1035445/141‑1 (MQ=255)

                                                                                                                                  ttgAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACACCGGTACTTTGGACTTTAAAGTTAAAGTTTTGTTCAATATGATCCTTTAATGCTTTCGCat        >  4:416977/1‑141 (MQ=255)

                                                                                                                                     aaaCTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACACCGGTACTTTGGACTTTAAAGTTAAAGTTTTGTTCAATATGATCCTTTAATGCTTTCGCattat     <  1:718958/141‑1 (MQ=255)

                                                                                                                                      aaCTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACACCGGTACTTTGGACTTTAAAGTTAAAGTTTTGTTCAATATGATCCTTTAATGCTTTCGCattatt    <  1:159398/141‑1 (MQ=255)

                                                                                                                                       aCTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACACCGGTACTTTGGACTTTAAAGTTAAAGTTTTGTTCAATATGATCCTTTAATGCTTTCGCATTAttt   <  1:527197/141‑1 (MQ=255)

GAGCGTAATGGATTATTATGCCTTTGATTTTACTTGAATATAGCTGTGATTTGATCAACCTGTTCTTTATTTAAAGGGTGTTTAGATAGCTCTATGGCTTCGCTAATAATACGTCGTAACGAAGATTTTGTTGAAACTGTATCAACATATTTATCTCTTTCTTCAGAACCTTGAATGATATTAACTGTACCATCACTAAAGTAAATAACACCGGTACTTTGGACTTTAAAGTTAAAGTTTTGTTCAATATGATCCTTTAATGCTTTCGCATTATTTG  >  USA300TCH1516_ALE/1353921‑1354197

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`