| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 1,433,241 | A→T | 5.6% | A77A (GCA→GCT) | dapA → | 4‑hydroxy‑tetrahydrodipicolinate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 1,433,241 | 0 | A | T | 5.6% | 284.3 / 7.2 | 89 | A77A (GCA→GCT) | dapA | 4‑hydroxy‑tetrahydrodipicolinate synthase |
| Reads supporting (aligned to +/- strand): ref base A (47/37); new base T (3/2); total (50/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.23e-01 | |||||||||||
ATTTTTTACTAGAAAATAATGCCCAAGCAATCATCGTTAATGGAACTACTGCTGAGAGCCCTACTTTAACAACAGATGAAAAAGAACGCATTCTAAAAACAGTTATTGATCTTGTAGATAAACGTGTTCCTGTCATAGCAGGAACTGGCACTAATGATACTGAAAAGTCAATCCAAGCTTCAATCCAAGCTAAAGCCTTAGGGGCTGATGCAATTATGTTAATTACGCCCTACTACAACAAAACGAACCAACGTGGTTTAGTCAAACACTTTGAAG > USA300TCH1516_ALE/1433102‑1433377 | aTTTTTTACTAGAAAATAATGCCCAAGCAATCATCGTTAATGGAACTACTGCTGAGAGCCCTACTTTAACAACAGATGAAAAAGAACGCATTCTAAAAACAGTTATTGATCTTGTAGATAAACGTGTTCCTGTCATAGCAg < 2:997820/141‑1 (MQ=255) tttACTAGAAAATAAAGCCCAAGCAATCATCGTTAATGGAACTACTGCTGAGAGCCCTACTTTAACAACAAAAAAAAAAGAACGCATTCTAAAAACAGTTATTGATCTTGTAGATAAACGTGTTCCTGTCATAGCAGGAAc < 2:693219/141‑1 (MQ=255) tACTAGAAAATAATGCCCAAGCAATCATCGTTAATGGAACTACTGCTGAGAGCCCTACTTTAACAACAGATGAAAAAGAACGCATTCTAAAAACAGTTATTGATCTTGTAGATAAACGTGTTCCTGTCATAGCTGGAACTg < 1:375900/141‑1 (MQ=255) naCTAGAAAATAATGCCNAAGCAATCATNGTTANTGGNACTACTGCTGAGAGCCCTACTTTAACAACAGATGAAAAAGAACGCATTCTAAAAACAGTTATTGATCTTGTAGATAAACGTGTTCCTGTCATAGCAGGAACTg > 1:740434/2‑141 (MQ=255) aGAAAATAATGCCCAAGCAATCATCGTTAATGGAACTACTGCTGAGAGCCCTACTTTAACAACAGATGAAAAAGAACGCATTCTAAAAACAGTTATTGATCTTGTAGATAAACGTGTTCCTGTCATAGCAGGAACTGGCAc > 4:110192/1‑141 (MQ=255) aGAAAATAATGCCCAAGCAATCAACGTTAATGGAACTACTGCTGAGAGCCCTACTTTAACAACAGATGAAAAAGAACGCATTCTAAAAACAGTTATTGATCTTGTAGATAAACGTGTTCCTGTCATAGCAGGAACTGGCAc < 4:252102/141‑1 (MQ=255) gAAAATAATCCCCAAGCAATCATCGTTAATGGCACTACTGCTGAGAGCCCTACTTTAACAACAGATGAAAAAGAACGCATTCTAAAAACAGTTATTGATCTTGTAGATAAACGTGTTCCTGTCATAGCAGGAACTGGCACt < 2:839116/141‑1 (MQ=255) taatGCCCAAGCAATCATCGTTAATGGAACTACTGCTGAGAGCCCTACTTTAACAACAGATGAAAAAGAGCGCATTCTAAAAACAGTTATTGATCTTGTAGATAAACGTGTTCCTGTCATAGCAGGAACTGGCACTAATGa < 1:439768/141‑1 (MQ=255) taatGCCCAAGCAATCATCGTTAATGGAACTACTGCTGAGAGCCCTACTTTAACAACAGATGAAAAAGAACGCATTCTAAAAACAGTTATTGATCTTGTAGATAAACGTGTTCCTGTCATAGCAGGAACTGGCACTAATGa < 4:73113/141‑1 (MQ=255) taatGCCCAAGCAATCATCGTTAATGGAACTACTGCTGAGAGCCCTACTTTAACAACAGATGAAAAAGAACGCATTCTAAAAACAGTTATTGATCTTGTAGATAAACGTGTTCCTGTCATAGCAGGAACTGGCACTAATGa < 1:823920/141‑1 (MQ=255) atGCCCAAGCAATCATCGTTAATGGAACTACTGCTGAGAGCCCTACTTTAACAACAGATGAAAAAGAACGCATTCTAAAAACAGTTATTGATCTTGTAGATAAACGTGTTCCTGTCATAGCAGGAACTGGCACTAATGATa > 2:1071718/1‑141 (MQ=255) atGCCCAAGCAATCATCGTTAATGGAACTACTGCTGAGAGCCCTACTTTAACAACAGATGAAAAAGAACGCATTCTAAAAACAGTTATTGATCTTGTAGATAAACGTGTTCCTGTCATAGCAGGAACTGGCACTAATGATa > 2:198267/1‑141 (MQ=255) atGCCCAAGCAATCATCGTTAATGGAACTACTGCTGAGAGCCCTACTTTAACAACAGATGAAAAAGAACGCATTCTAAAAACAGTTATTGATCTTGTAGATAAACGTGTTCCTGTCATAGCAGGAACTGGCACTAATGATa < 1:65749/141‑1 (MQ=255) atGCCCAAGCAATCATCGTTAATGGAACTACTGCTGAGAGCCCTACTTTAACAACAGATGAAAAAGAACGCATTCTAAAAACAGTTATTGATCTTGTAGATAAACGTGTTCCTGTCATAGCAGGAACTGGCACTAATGATa > 2:902184/1‑141 (MQ=255) atGCCCAAGCAATCATCGTTAATGGAACTACTGCTGAGAGCCCTACTTTAACAACAGATGAAAAAGAACGCATTCTAAAAACAGTTATTGATCTTGTAGATAAACGTGTTCCTGTCATAGCAGGAACTGGCACTAATGATa > 4:235651/1‑141 (MQ=255) atGCCCAAGCAATCATCGTTAATGGAACTACTGCTGAGAGCCCTACTTTAACAACAGATGAAAAAGAACGCATTCTAAAAACAGTTATTGATCTTGTAGATAAACGTGTTCCTGTCATAGCAGGAACTGGCACTAATGATa > 3:252769/1‑141 (MQ=255) gCCCAAGCAATCATCGTTAATGGAACTACTGCTGAGAGCCCTACTTTAACAACAGATGAAAAAGAACGCATTCTAAAAACAGTTATTGATCTTGTAGATAAACGTGTTCCTGTCATAGCTGGAACTGGAACTAATGATACt > 3:197646/1‑141 (MQ=255) cccAAGCAATCATCGTTAATGGAACTACTGCTGAGAGCCCTACTTTAACAACAGATGAAAAAGAACGCATTCTAAAAACAGTTATTGATCTTGTAGATAAACGTGTTCCTGTCATAGCAGGAACTGGCACTAATGATAATg > 3:225618/1‑141 (MQ=255) ccAAGCAATCATCGTTAATGGAACTACTGCTGAGAGCCCTACTTTAACAACAGATGAAAAAGAACGCATTCTAAAAACAGTTATTGATCTTGTAGATAAACGTGTTCCTGTCATAGCAGGAACTGGCACTAATGATACTGa < 2:505441/141‑1 (MQ=255) aaGCAATCATCGTTAATGGAACTACTGCTGAGAGCCCTACTTTAACAACAGATGAAAAAGAACGCATTCTAAAAACAGTTATTGATCTTGTAGATAAACGTGTTCCTGTCATAGCAGGAACTGGCACTAATGATACTGaaa < 1:239072/141‑1 (MQ=255) gCAATCATCGTTAATGGAACTACTGCTGAGAGCCCTACTTTAACAACAGATGAAAAAGAACGCATTCTAAAAACAGTTATTGATCTTGTAGATAAACGTGTTCCTGTCATAGCAGGAACTGGCACTAATGATACTGAAAAg > 1:506171/1‑141 (MQ=255) cAATCATCGTTAATGGAACTACTGCTGAGAGCCCTACTTTAACAACAGATGAAAAAGAACGCATTCTAAAAACAGTTATTGATCTTGTAGATAAACGTGTTCCTGTCATAGCAGGAACTGGCACTAATGATACTGAAAAGt < 1:173551/141‑1 (MQ=255) catcGTTAATGGAACTACTGCTGAGAGCCCTACTTTAACAACAGATGAAAAAGAACGCATTCTAAAAACAGTTATTGATCTTGTAGATAAACGTGTTCCTGTCATAGCAGGAACTGGCACTAATGATACTGAAAAGtcaat < 2:583420/141‑1 (MQ=255) atcGTTAATGGAACTACTGCTGAGAGCCCTACTTTAACAACAGATGAAAAAGAACGCATTCTAAAAACAGTTATTGATCTTGTAGATAAACGTGTTCCTGTCATAGCTGGAACTGGAACTAATGATACTGAAAAGtcaatc > 1:1080298/1‑141 (MQ=255) tcGTTAATGGAACTACTGCTGAGAGCCCTACTTTAACAACAGATGAAAAAGAACGCATTCTAAAAACAGTTATTGATCTTGTAGATAAACGTGTTCCTGTCATAGCAGGAACTGGCACTAATGATACTGAAAAGtcaatcc > 1:166651/1‑141 (MQ=255) cGTTAATGGAACTACTGCTGAGAGCCCTACTTTAACAACAGATGAAAAAGAACGCATTCTAAAAACAGTTATTGATCTTGTAGATAAACGTGTTCCTGTCATAGCAGGAACTGGCACTAATGATACTGAAAAGtcaatcca < 1:891265/141‑1 (MQ=255) gTTAATGGAACTACTGCTGAGAGCCCTACTTTAACAACAGATGAAAAAGAACGCATTCTAAAAACAGTTATTGATCTTGTAGATAAACGTGTTCCTGTCATAGCAGGAACTGGCACTCATGATACTGAAAAGtcaatccaa > 2:630250/1‑141 (MQ=255) aaTGGAACTACTGCGTAGAGCCCTACTTAAACAACAGATGAAAAAGAACGCATTCTAAAAACATTTTTTGATCTTGTAGATAAACTTGTTCCTGTCATAGCAGGAACTGGCACTAATGATACTGAAAAGtcaatccaagct < 2:835007/141‑1 (MQ=255) tGGAACTACTGCTGAGAGCCCTACTTTAACAACAGATGAAAAAGAACGCATTCTAAAAACAGTTATTGATCTTGTAGATAAACGTGTTCCTGTCATAGCAGGAACTGGCACTAATGATACTGAAAAGtcaatcaaagcttc > 4:394826/1‑141 (MQ=255) tGGAACTACTGCTGAGAGCCCTACTTTAACAACAGATGAAAAAGAACGCATTCTAAAAACAGTTATTGATCTTGTAGATAAACGTGTTCCTGTCATAGCAGGAACCGGCACTAATGATACTGAAAAGtcaatccaagcttc < 2:524500/141‑1 (MQ=255) actactGCTGAGAGCCCTACTTTAACAACAGATGAAATAGAACGCATTCTAAAAACAGTTATTGATCTTGTAGATAAACGTGTTCCTGTCATAGCAGGAACTGGCACTAATGATACTGAAAAGtcaatccaagcttcaatc > 1:781810/1‑141 (MQ=255) ctactGCTGAGAGCCCTACTTTAATTACAGATGAAAAAGAACGCATTCTAAAAACAGTTATTGATCTTGTAGATAAACGTGTTCCTGTCATAGCAGGAACTGGCACTAATGATACTGAAAAGtcaatccaagctccaatcc > 1:254105/1‑141 (MQ=255) ctactGCTGAGAGCCCTACTTTAACAACAGATGAAAAAGAACGCATTCTAAAAACAGTTATTGATCTTGTAGATAAACGTGTTCCTGTCATAGCAGGAACTGGCACTAATGATACTGAAAAGtcaatccaagcttcaatcc > 1:333761/1‑141 (MQ=255) tactGCTGAGAGCCCTACTTTAACAACAGATGAAAAAGAACGCATTCTAAAAACAGTTATTGATCTTGTAGATAAACGTGTTCCTGTCATAGCAGGAACTGGCACTAATGATACTGAAAAGtcaatccaagcttcaatcca > 1:447362/1‑141 (MQ=255) gctgAGAGCCCTACTTTAACAACAGATGAAAAAGAACGCATTCTAAAAACAGTTATTGATCTTGTAGATAAACGTGTTCCTGTCATAGCAGGAACTAGCACTAATGATACTGAAAAGtcaatccaagcttcaatccaagct > 4:414175/1‑141 (MQ=255) ctgAGAGCCCTACTTTAACAACAGATGAAAAAGAACGCATTCTAAAAACAGTTATTGATCTTGTAGATAAACGTGTTCCTGTCATAGCAGGAACTGGCACTAATGATACTGAAAAGTCAATCCAAGCTTCAATCCAAGCTa > 1:295135/1‑141 (MQ=255) tgAGAGCCCTACTTTAACAACAGATGAAAAAGAACGCATTCTAAAAACAGTTATTGATCTTGTAGATAAACGTGTTCCTGTCATAGCAGGAACTGGCACTAATGATACTGAAAAGTCAATCCAAGCTTCAATCCAAGCTaa > 1:557258/1‑141 (MQ=255) gtgCCCTACTATAACAACAGATGAAAAATAACGCATTCTAAAAACAGTTATTGATCTTGTAGATAAACGTGTTCCTGTCATAGCAGGAACTGGCACTAATGATACTGAAAAGTCAATCCAAGCTTCAATCCAAGCTAAAGc < 2:281201/139‑1 (MQ=255) gCCCTACTTTAACAACAGATGAAAAAGAACGCATTCTAAAAACAGTTATTGATCTTGTAGATAAACGTGTTCCTGTCATAGCAGGAACTGGCACTAATGATACTGAAAAGTCAATCCAAGCTTCAATCCAAGCTAAAGCCt > 1:1072379/1‑141 (MQ=255) cTACTTTAACAACAGATGAAAAAGAACGCATTCTAAAAACAGTTATTGATCTTGTAGATAAACGTGTTCCTGTCATAGCAGGAACTGGCACTAATGATACTGAAAAGTCAATCCAAGCTTCAATCCAAGCTAAAGCCTTAg < 1:24448/141‑1 (MQ=255) cTTTAACAACAGATGAAAAAGAACGCATTCTAAAAACAGTTATTGATCTTGTAGATAAACGTTTTCCTGTCATAGCAGGATGTGGCACTAATAATACTGAAAAGTCAAACCTAGCTTCAATCCAAGCTAAAGCCTTAgggg > 2:479293/1‑141 (MQ=255) cTTTAACAACAGATGAAAAAGAACGCATTCTAAAAACAGTTATTGATCTTGTAGATAAACGTGTTCCTGTCATAGCAGGAACTGGCACTAATGATACTGAAAAGTCAATCCAAGCTTCAATCCAAGCTAAAGCCTTAgggg < 1:191520/141‑1 (MQ=255) ttAACAACAGATGAAAAAGAACGCATTCTAAAAACAGTTACTGTTCTTGTAGATAAACGTGTTCCTGTCATAGCAGGAACTGGCACTAATGATACTGAAAAGTCAATCCAAGCTTCAATCCAAGCTAAAGCCTTAGGGGCt < 1:39807/141‑1 (MQ=255) aacaacAGATGAAAAAGAACGCATTCTAAAAACAGTTATTGATCTTGTAGATAAACGTGTTCCTGTCATAGCAGGAACTGGCACTAATGATACTGAAAAGTCAATCCAAGCTTCAATCCAAGCTAAAGCCTTAGGGGCTGa > 4:425033/1‑141 (MQ=255) aacaacAGATGAAAAAGAACGCATTCTAAAAACAGTTATTGATCTTGTAGATAAACGTGTTCCTGTCATAGCAGGAACTGGCACTAATGATACTGAAAAGTCAATCCAAGCTTCAATCCAAGCTAAAGCCTTAGGGGCTGa < 3:190172/141‑1 (MQ=255) acaacaGATGAAAAAGAACGCATTCTAAAAACAGTTATTGATCTTGTAGATAAACGTGTTCCTGTCATAGCAGGAACTGGCACTAATGATACTGAAAAGTCAATCCAAGCTTCAATCCAAGCTAAAGCCTTAGGGGCTGAt < 1:442702/141‑1 (MQ=255) aGATGAAAAAGAACGCATTCTAAAAACAGTTATTGATCTTGTAGATAAACGTGTTCCTGTCATAGCAGGAACTGGCACTAATGATACTGAAAAGTCAATCCAAGCTTCAATCCAAGCTAAAGCCTTAGGGGCTGATGCAAt > 2:284031/1‑141 (MQ=255) aGATGAAAAAGAACGCATTCTAAAAACAGTTATTGATCTTGTAGATAAACGTGTTCCTGTCATAGCAGGAACTGGCACTAATGATACTGAAAAGTCAATCCAAGCTTCAATCCAAGCTAAAGCCTTAGGGGCTGATGCAAt > 1:611156/1‑141 (MQ=255) aGATGAAAAAGAACGCATTCTAAAAACAGTTATTGATCTTGTAGATAAACGTGTTCCTGTCATAGCAGGAACTGGCACTAATGATACTGAAAAGTCAATCCAAGCTTCAATCCAAGCTAAAGCCTTAGGGGCTGATGCAAt > 1:608378/1‑141 (MQ=255) tGAAAAAGAACGCATTCTAAAAACAGTTATTGATCTTGTAGATAAACGTGTTCCTGTCATAGCAGGAACTGGCACTAATGATACTGAAAAGTCAATCCAAGCTTCAATCCAAGCTAAAGCCTTAGGGGCTGATGCAATTAt < 1:284661/141‑1 (MQ=255) aaaaGAACGCATTCTAAAAACAGTTATTGATCTTGTAGATAAACGTGTTCCTGTCATAGCAGGAACTGGCACTAATGATACTGAAAAGTCAATCCAAGCTTCAATCCAAGCTAAAGCCTTAGGGGCTGATGCAATTATGtt > 3:232948/1‑141 (MQ=255) aaaaGAACGCATTCTAAAAACAGTTATTGATCTTGTAGATAAACGTGTTCCTGTCATAGCAGGAACTGGCACTAATGATACTGAAAAGTCAATCCAAGCTTCAATCCAAGCTAAAGCCTTAGGGGCTGATGCAATTATGtt > 2:1061353/1‑141 (MQ=255) aaGAACGCATTCTAAAAACAGTTATTGATCTTGTAGATAAACGTGTTCCTGTCATAGCAGGAACTGGCACTAATGATACTGAAAAGTCAATCCAAGCTTCAATCCAAGCTAAAGCCTTAGGGGCTGATGCAATTATGTTaa > 2:264732/1‑141 (MQ=255) aaCGCATTCTAAAAACAGTTATTGATCTTGTAGATAAACGTGTTCCTGTCATAGCAGGAACTGGCACTAATGATACTGAAAAGTCAATCCAAGCTTCAATCCAAGCTAAAGCCTTAGGGGCTGATGCAATTATGTTAATTa < 1:662379/141‑1 (MQ=255) aCGCATTCTAAAAACAGTTATTGATCTTGTAGATAAACGTGTTCCTGTCATAGCAGGAACTGGCACTAATGATACTGAAAAGTCAATCCAAGCTTCAATCCAAGCTAAAGCCTTAGGGGCTGATGCAATTATGTTAATTAc > 1:924072/1‑141 (MQ=255) cATTCTAAAAACAGTTATTGATCTTGTAGATAAACGTGTTCCTGTCATAGCTGGAACTGGAACTAATGATACTGAAAAGTCAATCCAAGCTTCATTCCAAGCTAAAGCCTTAGGGGCTGATGCAATTATGTTAATTACGcc < 2:537148/141‑1 (MQ=255) aTTCTAAAAACAGTTATTGATCTTGTAGATAAACGTGTTCCTGTCATAGCAGGAACTGGCACTAATGATACTGAAAAGTCAATCCAAGCTTCAATCCAAGCTAAAGCCTTAGGGGCTGATGCAATTATGTTAATTACGccc > 1:1026821/1‑141 (MQ=255) aTTCTAAAAACAGTTATTGATCTTGTAGATAAACGTGTTCCTGTCATAGCAGGAACTGGCACTAATGATACTGAAAAGTCAATCCAAGCTTCAATCCAAGCTAAAGCCTTAGGGGCTGATGCAATTATGTTAATTACGccc > 2:932166/1‑141 (MQ=255) tctcAAAACAGTTACTGATGTTGTAGATAAACGTGTTCCTGTCATAGCAGGAACTGGCACTAATGATACTGAAAAGTCAATCCAAGCTTCAATCCAAGCTAAAGCCTTAGGGGCTGATGCAATTATGTTAATTACGCCcta < 4:313622/141‑1 (MQ=255) cTAAAAACAGTTATTGATCTTGTAGATAAACGTGTTCCTGTCATAGCAGGAACTGGCACTAATGATACTGAAAAGTCAATCCAAGCTTCAATCCAAGCTAAAGCCTTAGGGGCTGATGCAATTATGTTAATTACGCCctac > 3:413621/1‑141 (MQ=255) tAAAAACAGTTATTGATCTTGTAGATAAACGTGTTCCTGTCATAGCAGGAACTGGCACTAATGATACTGAAAAGTCAATCCAAGCTTCAATCCAAGCTAAAGCCTTAGGGGCTGATGCAATTATGTTAATTACGCCctact > 2:284672/1‑141 (MQ=255) tAAAAACAGTTATTGATCTTGTAGATAAACGTGTTCCTGTCATAGCAGGAACTGGCAATAAGGATACTGAAAAGTCAATCCAAGCTTCAATCCAAGCTAAAGCCTTAGGGGCTGATGCAATTATGTTAATTACGCCctact < 1:564910/141‑1 (MQ=255) aaaaaCAGTTATTGATCTTGTAGATAAACGTGTTCCTGTCATAGCAGGAACTGGCACTAATGATACTGAAAAGTCAATCCAAGCTTCAATCCAAGCTAAAGCCTTAGGGGCTGATGCAATTATGTTAATTACGCCctacta > 1:303124/1‑141 (MQ=255) aaaCAGTTATTGATCTTGTAGATAAACGTGTTCCTGTCATAGCAGGAACTGGCACTAATGATACTGAAAAGTCAATCCAAGCTTCAATCCAAGCTAAAGCCTTAGGGGCTGATGCAATTATGTTAATTACGCCCTACTaca > 3:209100/1‑141 (MQ=255) aCAGTTATTGATCTTGTAGATAAACGTGTTCCTGTCATAGCAGGAACTGGCACTAATGATACTGAAAAGTCAATCCAAGCTTCAATCCAAGCTAAAGCCTTAGGGGCTGATGCAATTATGTTAATTACGCCCTACTacaac > 3:410690/1‑141 (MQ=255) ttATTGATCTTGTAGATAAACGTGTTCCTGACATAGCTGGAACTGGAACTAATGATACTGAAAAGTCAATCCAAGCTTCATTCCAAGCTAAAGCCTTAGGGGCTGATGCAATTATGTTAATTACGCCCTACTACAACaaaa > 4:409536/1‑141 (MQ=255) aTCTTGTAGATAAACGTGTTCCTGTCATAGCAGGAACTGGCACTAATGATACTGAAAAGTCAATCCAAGCTTCAATCCAAGCTAAAGCCTTAGGGGCTGATGCAATTATGTTAATTACGCCCTACTACAACAAAACGAAcc < 2:506171/141‑1 (MQ=255) cTTGTAGATAAACGTGTTCCTGTCATAGCAGGAACTGGCACTAATGATACTGAAAAGTCAATCCAAGCTTCAATCCAAGCTAAAGCCTTAGGGGCTGATGCAATTATGTTAATTACGCCCTACTACAACAAAACGAACCaa < 2:437447/141‑1 (MQ=255) cTTGTAGATAAACGTGTTCCTGTCATAGCAGGAACTGGCACTAATGATACTGAAAAGTCAATCCAAGCTTCAATCCAAGCTAAAGCCTTAGGGGCTGATGCAATTATGTTAATTACGCCCTACTACAACAAAACGAACCaa < 1:119092/141‑1 (MQ=255) cTTGTAGATAAACGTGTTCCTGTCATAGCAGGAACTGGCACTAATGATACTGAAAAGTCAATCCAAGCTTCAATCCAAGCTAAAGCCTTAGGGGCTGATGCAATTATGTTAATTACGCCCTACTACAACAAAACGAACCaa < 3:172810/141‑1 (MQ=255) tGTAGATAAACGTGTTCCTGTCATAGCAGGAACTGGCACTAATGATACTGAAAAGTCAATCCAAGCTTCAATCCAAGCTAAAGCCTTAGGGGCTGATGCAATTATGTTAATTACGCCCTACTACAACAAAACGAACCAACg < 2:910052/141‑1 (MQ=255) gTAGATAAACGTGTTCCTGTCATAGCAGGAACTGGCACTAATGATACTGAAAAGTCAATCCAAGCTTCAATCCAAGCTAAAGCCTTAGGGGCTGATGCAATTATGTTAATTACGCCCTACTACAACAAAACGAACCAACGt > 1:96964/1‑141 (MQ=255) gTAGATAAACGTGTTCCTGTCATAGCAGGAACTGGCACTAATGATACTGAAAAGTCAATCCAAGCTTCAATCCAAGCTAAAGCCTTAGGGGCTGATGCAATTATGTTAATTACGCCCTACTACAACAAAACGAACCAACGt < 1:425599/141‑1 (MQ=255) tAGATAAACGTGTTCCTGTCATAGCAGGAACTGGCACTAATGATACTGAAAAGTCAATCCAAGCTTCAATCCAAGCTAAAGCCTTAGGGGCTGATGCAATTATGTTAATTACGCCCTACTACAACAAAACGAACCAACGTg < 2:375043/141‑1 (MQ=255) gATAAACGTGTTCCTGTCATAGCAGGAACTGGCACTAATGATACTGAAAAGTCAATCCAAGCTTCAATCCAAGCTAAAGCCTTAGGGGCTGATGCAATTATGTTAATTACGCCCTACTACAACAAAACGAACCAACGTGGt > 3:196826/1‑141 (MQ=255) aTAAACGTGTTCCTGTCATAGCAGGAACTGGCACTAATGATACTGAAAAGTCAATCCAAGCTTCAATCCAAGCTAAAGCCTTAGGGGCTGATGCAATTATGTTAATTACGCCCTACTACAACAAAACGAACCAACGTGGtt > 1:334799/1‑141 (MQ=255) gtgccagtTCCTGTCATAGCAGGAACTGGCACTAATGATACTGAAAAGTCAATCCAAGCCTCAATCCAAGCTAAAGCCTTAGGGGCTGATGCAATTATGTTAATTACGCACTACTACAGCAAAACGAACCAACGTGGGTTa > 2:876389/7‑141 (MQ=255) aaCGTGTTCCTGTCATAGCAGGAACTGGCACTAATGATACTGAAAAGTCAATCCAAGCTTCAATCCAAGCTAAAGCCTTAGGGGCTGATGCAATTATGTTAATTACGCCCTACTACAACAAAACGAACCAACGTGGTTTAg > 2:569995/1‑141 (MQ=255) aCGTGTTCCTGTCATAGCAGGAACTGGCACTAATGATACTGAAAAGTCAATCCAAGCTTCAATCCAAGCTAAAGCCTTAGGGGCTGATGCAATTATGTTAATTACGCCCTACTACAACAAAACGAACCAACGTGGTTTAGt < 4:70931/141‑1 (MQ=255) tgtTCCTGTCATAGCAGGAACTGGCACTAATGATACTGAAAAGTCAATCCAAGCTTCAATCCAAGCTAAAGCCTTAGGGGCTGATGCAATTATGTTAATTACGCCCTACTACAACAAAACGAACCAACGTGGTTTAGTCaa < 4:14151/141‑1 (MQ=255) ttCCTGTCATAGCAGGCACTGGCACTAATGATACTGAAAAGTCAATCCAAGCTTCAATCCAAGCTAAAGCCATAGGGGCTGATGCAATTATGTTAATTACGCCCTACTACAACAAAACGAACCAACGTGGATTAGTCAAac > 2:61539/1‑141 (MQ=255) tGTCATAGCAGGAACTGGCACTAATGATACTGAAAAGTCAATCCAAGCTTCAATCCAAGCTAAAGCCTTAGGGGCTGATGCAATTATGTTAATTACGCCCTACTACAACAAAACGAACCCACGTGGTTTAGTCAAACACtt > 3:115153/1‑141 (MQ=255) gTCATAGCAGGAACTGGCACTAATGATACTGAAAAGTCAATCCAAGCTTCAATCCAAGCTAAAGCCTTAGGGGCTGATGCAATTATGTTAATTACGCCCTACTACAACAAAACGAACCAACGTGGTTTAGTCAAACACttt < 1:35849/141‑1 (MQ=255) ctatgacAGGAACTGGCACTAATGATACTGAAAAGTCAATCCAAGCTTCAATCCAAGCTAAAGCCTTAGGGGCTGATGCAATTATGTTAATTACGCCCTACTACAACAAAACGAACCAACGTGGTTTAGTCAAACACTTTg > 1:430575/7‑141 (MQ=255) cATAGCAGGAACTGGCACTAATGATACTGAAAAGTCAATCCAAGCTTCAATCCAAGCTAAAGCCTTAGGGGCTGATGCAATTATGTTAATTACGCCCTACTACAACAAAACGAACCAACGTGGTTTAGTCAAACACTTTGa > 1:520718/1‑141 (MQ=255) atgacAGGAACTGGCACTAATGATACTGAAAAGTCAATCCAAGCTTCAATCCAAGCTAAAGCCTTAGGGGCTGATGCAATTATGTTAATTACGCCCTACTACAACAAAACGAACCAACGTGGTTTAGTCAAACACTTTGaa > 3:240456/5‑141 (MQ=255) tAGCAGGAACTGGCACTAATGATACTGAAAAGTCAATCCAAGCTTCAATCCAAGCTAAAGCCTTAGGGGCTGATGCAATTATGTTAATTACGCCCTACTACAACAAAACGAACCAACGTGGTTTAGTCAAACACTTTGAAg > 3:279908/1‑141 (MQ=255) tAGCAGGAACTGGCACTAATGATACTGAAAAGTCAATCCAAGCTTCAATCCAAGCTAAAGCCTTAGGGGCTGATGCAATTATGTTAATTACGCCCTACTACAACAAAACGAACCAACGTGGTTTAGTCAAACACTTTGAAg < 2:244174/141‑1 (MQ=255) tAGCAGGAACTGGCACTAATGATACTGAAAAGTCAACCCAAGCTTCAATCCAAGCTAAAGCCTTAGGGGCTGATGCAATTATGTTAATTACGCCCTACTACAACAAAACGAACCAACGTGGTTTAGTCAAACACTTTGAAg < 3:249214/141‑1 (MQ=255) | ATTTTTTACTAGAAAATAATGCCCAAGCAATCATCGTTAATGGAACTACTGCTGAGAGCCCTACTTTAACAACAGATGAAAAAGAACGCATTCTAAAAACAGTTATTGATCTTGTAGATAAACGTGTTCCTGTCATAGCAGGAACTGGCACTAATGATACTGAAAAGTCAATCCAAGCTTCAATCCAAGCTAAAGCCTTAGGGGCTGATGCAATTATGTTAATTACGCCCTACTACAACAAAACGAACCAACGTGGTTTAGTCAAACACTTTGAAG > USA300TCH1516_ALE/1433102‑1433377 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |