BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	1,436,255	T→A	5.3%	T250T (ACT→ACA)	yxeP_3 →	putative hydrolase YxeP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,436,255	0	T	A	5.3%	413.7 / 9.3	132	T250T (ACT→ACA)	yxeP_3	putative hydrolase YxeP
Reads supporting (aligned to +/- strand): ref base T (65/60); new base A (4/3); total (69/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.26e-01

Only 100 of 135 total aligned reads displayed.

ACAACATGTATTAACTCAAATATCACAAATTCAACAATTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAGATTGTGAAGTTAAATTTGCAGAAGGTTATCCCCCTACAATCAATAGTCCGAA  >  USA300TCH1516_ALE/1436117‑1436395

  aacaTGTATTAACTCAAATATCACAAATTCAACAATTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTc                                                                                                                                          <  3:179934/141‑1 (MQ=255)

  aacaTGTATTAACTCAAATATCACAAATTCAACAATTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTc                                                                                                                                          >  2:968527/1‑141 (MQ=255)

       gTATTAACTCAAATATCACAAATTCAACAATTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACa                                                                                                                                     >  1:285447/1‑141 (MQ=255)

        tATTAACTCAAATATCACAAATTCAACAATTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACat                                                                                                                                    <  2:971303/141‑1 (MQ=255)

        tATTAACTCAAATATCACAAATTCAACAATTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTCTAACAGGTCTTGGCTTTTTAGTAGTTGCTATTCTTACat                                                                                                                                    >  3:359730/1‑141 (MQ=255)

         aTTAACTCAAATATCACAAATTCAACAATTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACata                                                                                                                                   <  1:1040134/141‑1 (MQ=255)

           tAACTCAAATATCACAAATTCGACAATTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATg                                                                                                                                 >  2:944628/1‑141 (MQ=255)

           tAACTCAAATATCACAAATTCAACAATTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGTTATTTAGAAGGTACAATTCGTACATATg                                                                                                                                 <  2:894346/141‑1 (MQ=255)

           tAACTCAAATATCACAAATTCAACAATTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATg                                                                                                                                 <  2:76249/141‑1 (MQ=255)

             aCTCAAATATCACAAATTCAACAATTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGat                                                                                                                               >  2:625836/1‑141 (MQ=255)

             aCTCAAAGATCACAAATTCAACAATTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGat                                                                                                                               >  3:360629/1‑141 (MQ=255)

              cTCAAATATCACAAATTCAACAATTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGCACAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGata                                                                                                                              >  1:19776/1‑141 (MQ=255)

              cTCAAATATCACAAATTCAACAATTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCCGCTCAGGAGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGata                                                                                                                              >  1:19661/1‑141 (MQ=255)

                  aaTATCACAAATTCAACAATTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATtga                                                                                                                          <  1:191962/141‑1 (MQ=255)

                  aaTATCACAAATTCAACAATTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATtga                                                                                                                          >  3:9911/1‑141 (MQ=255)

                          aaaTTCAACAATTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCACGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTaa                                                                                                                  >  2:338148/1‑141 (MQ=255)

                            aTTCAACAATTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTTATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACa                                                                                                                >  2:902999/1‑141 (MQ=255)

                            aTTCAACAATTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGTTATTTAGAAGGTACAATTCGTACATATGATATTGATGATTTAACa                                                                                                                >  2:921319/1‑141 (MQ=255)

                             ttCAACAATTTCACCTTATCGGTGTTAAACGAAATATTGTTCATATTGGTCATTTTAAAGGTGGTGAAGCGATTATCACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACaa                                                                                                               <  1:958179/141‑1 (MQ=255)

                             ttCAACAATTTCACCTTAACGGTTTGAAACGAAATATTGTTTATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAGGGTACTATTCGTACATATGATATTGATGATTTAACaa                                                                                                               <  1:10729/141‑1 (MQ=255)

                                aacaaTTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCg                                                                                                            <  4:234488/141‑1 (MQ=255)

                                 acaaTTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGTTATTTAGAAGGTACAATTCGTACATATGATATTGATGATTTAACAATCGt                                                                                                           >  2:274506/1‑141 (MQ=255)

                                 acaaTTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGt                                                                                                           >  2:805951/1‑141 (MQ=255)

                                   aaTTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTa                                                                                                         <  1:659385/141‑1 (MQ=255)

                                      ttCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTaaaa                                                                                                      >  4:202526/1‑141 (MQ=255)

                                       tCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTaaggt                                                                                                     >  1:835042/1‑138 (MQ=255)

                                       tCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTaaaaa                                                                                                     <  3:210269/141‑1 (MQ=255)

                                         aCCTTAACGGTTTGAATTGCTATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATc                                                                                                   <  1:283491/141‑1 (MQ=255)

                                         aCCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATc                                                                                                   >  2:260595/1‑141 (MQ=255)

                                          ccTTAACGGTTTGAAACGAAATATTGTTCATATTAGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATTGCTATTTAGAAGGTACTATTCGTACATATGATATTGACGATTTAACAATCGTTAAAAATCa                                                                                                  <  4:231578/141‑1 (MQ=255)

                                           cgtAACGGGTTGAAACGAAATATTGTTCATATTGGTCATTTGAAAGCTGGTGAAGCGATGAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCaa                                                                                                 >  4:11503/3‑141 (MQ=255)

                                               aCGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTGGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGc                                                                                             <  2:153198/141‑1 (MQ=255)

                                                cGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGca                                                                                            <  1:802186/141‑1 (MQ=255)

                                                    ttGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAg                                                                                        >  1:211814/1‑141 (MQ=255)

                                                     tGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGa                                                                                       >  1:229359/1‑141 (MQ=255)

                                                     tGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGAGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAAGCGTTAAAAATCAAATGCACAAGa                                                                                       <  2:89245/141‑1 (MQ=255)

                                                       aaaCGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATa                                                                                     <  1:775335/141‑1 (MQ=255)

                                                       aaaCGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATa                                                                                     <  1:610617/141‑1 (MQ=255)

                                                           gAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAg                                                                                 >  1:291901/1‑141 (MQ=255)

                                                            aaaTATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGa                                                                                <  1:835973/141‑1 (MQ=255)

                                                            aaaTATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGa                                                                                <  4:111669/141‑1 (MQ=255)

                                                               tatTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAg                                                                             >  2:478465/1‑141 (MQ=255)

                                                                atTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAgt                                                                            <  1:668398/141‑1 (MQ=255)

                                                                atTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAgt                                                                            >  2:525647/1‑141 (MQ=255)

                                                                 ttGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATTATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCATAAAgtg                                                                           >  2:430060/1‑141 (MQ=255)

                                                                 ttGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAgtg                                                                           >  4:424134/1‑141 (MQ=255)

                                                                 ttGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAgtg                                                                           >  2:430042/1‑141 (MQ=255)

                                                                  tGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAgtgt                                                                          <  1:1025779/141‑1 (MQ=255)

                                                                     tCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCaa                                                                       >  1:834242/1‑141 (MQ=255)

                                                                         atTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCtt                                                                   <  3:171482/141‑1 (MQ=255)

                                                                           tGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCt                                                                 <  1:308371/141‑1 (MQ=255)

                                                                            ggTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTg                                                                >  2:1078274/1‑141 (MQ=255)

                                                                             gTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGt                                                               >  2:943557/1‑141 (MQ=255)

                                                                               cATTTTAAAGCTGGTGAAGTGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGttt                                                             >  2:326184/1‑141 (MQ=255)

                                                                               cATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGttt                                                             <  2:786392/141‑1 (MQ=255)

                                                                                aTTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTa                                                            >  2:983752/1‑141 (MQ=255)

                                                                                     agaGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTa                                                       <  2:706234/139‑1 (MQ=255)

                                                                                      aaGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAg                                                      <  3:239943/141‑1 (MQ=255)

                                                                                      aaGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAg                                                      >  3:261825/1‑141 (MQ=255)

                                                                                       aGCTGGTGAAGCGATTAACACTGTACGAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACACTCGTTAAAACTCAAATGCACCAGATAGCAGCAAGTGTCAAGCTTCCGTTTAATGTAGa                                                     >  1:48165/1‑141 (MQ=255)

                                                                                        gCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAGAt                                                    <  3:333204/141‑1 (MQ=255)

                                                                                         cTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAGAtt                                                   >  1:1036529/1‑141 (MQ=255)

                                                                                           ggTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAGATtgt                                                 >  1:11613/1‑141 (MQ=255)

                                                                                             ttaaGCGATTAAATCTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGACAGTGTCAAGCTTCTGTTTAATGTAGATTGTGa                                               <  4:339582/139‑1 (MQ=255)

                                                                                             tGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAGATTGTGa                                               <  2:760327/141‑1 (MQ=255)

                                                                                              gAAGCGATTAACGCTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAGATTGTGaa                                              <  4:201512/141‑1 (MQ=255)

                                                                                               aaGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGCACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAGATTGTGAAg                                             <  4:403006/141‑1 (MQ=255)

                                                                                                aGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAGATTGTGAAGt                                            >  4:90349/1‑141 (MQ=255)

                                                                                                aGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAGATTGTGAAGt                                            <  2:823899/141‑1 (MQ=255)

                                                                                                     ttAACACTGTACCAAGTAGCGGCTATCTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATACACAAGATAGCAGAAAGAGTCAAGCTTCTGTGTAATGTAGATTGCGAAGTTaaaa                                       >  4:71643/1‑140 (MQ=255)

                                                                                                     ttAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAGATTGTGAAGTTAAAt                                       >  3:14345/1‑141 (MQ=255)

                                                                                                     ttAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAGATTGTGAAGTTAAAt                                       >  3:14352/1‑141 (MQ=255)

                                                                                                     ttAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAGATTGTGAAGTTAAAt                                       <  2:997806/141‑1 (MQ=255)

                                                                                                     ttAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAGATTGTGAAGTTAAAt                                       <  1:776066/141‑1 (MQ=255)

                                                                                                      tAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAGATTGTGAAGTTAAAtt                                      >  3:270275/1‑141 (MQ=255)

                                                                                                       aaCACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAGATTGTGAAGTTAAAttt                                     >  1:408057/1‑141 (MQ=255)

                                                                                                       aaCACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAGATTGTGAAGTTAAAttt                                     >  4:394634/1‑141 (MQ=255)

                                                                                                        acacTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAGATTGTGAAGTTAAATTTg                                    >  1:852333/1‑141 (MQ=255)

                                                                                                              tACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAGATTGTGAAGTTAAATTTGCAGAAg                              <  1:128220/141‑1 (MQ=255)

                                                                                                                 cAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAGATTGTGAAGTTAAAATTGCAGAAGGtt                           >  3:181934/1‑141 (MQ=255)

                                                                                                                    gttattgCTATGGAGAAGGTACTATTCGTACATATTATATTGATGATTTAACTATCGGTAAAAATCAAATGCACAAGATAGCAGAAAGTGGCAAGCTTCTGTTTAATGTGTATTGTGTTGTTAAATTTGCAGAAGGTTATc                        <  2:281279/135‑1 (MQ=255)

                                                                                                                    gTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAGATTGTGAAGTTAAATTTGCAGAAGGTTATc                        >  1:653993/1‑141 (MQ=255)

                                                                                                                       aTGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAGATTGTGAAGTTAAATTTGCAGAAGGTTATcccc                     <  4:196727/141‑1 (MQ=255)

                                                                                                                         ggCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAGATTGTGAAGTTAAATTTGCAGAAGGTTATCCCCCt                   >  2:550571/1‑141 (MQ=255)

                                                                                                                          gttATTTAGAAGGTACAATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAGAATGTGAAGTTAAATTTGCAGAAGGTTATCCCCCTa                  <  2:1050070/139‑1 (MQ=255)

                                                                                                                          gttATTTAGAAGGTACAATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAGAATGTGAAGTTAAATTTGCAGAAGGTTATCCCCCTa                  <  2:1049995/139‑1 (MQ=255)

                                                                                                                          gCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAGATTGTGAAGTTAAATTTGCAGAAGGTTATCCCCCTa                  <  1:377787/141‑1 (MQ=255)

                                                                                                                          gCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAGATTGTGAAGTTAAATTTGCAGAAGGTTATCCCCCTa                  >  2:293582/1‑141 (MQ=255)

                                                                                                                           cTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAGATTGTGAAGTTAAATTTGCAGAAGGTTATCCCCCTAc                 >  1:562012/1‑141 (MQ=255)

                                                                                                                                tAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAGATTGTGAAGTTAAATTTGCAGAAGGTTATCCCCCTAcaatca            >  1:848934/1‑141 (MQ=255)

                                                                                                                                 aGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAGATTGTGAAGTTAAATTTGCAGAAGGTTATCCCCCTAcaatcaa           >  1:305693/1‑141 (MQ=255)

                                                                                                                                  gAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAGATTGTGAAGTTAAATTTGCAGAAGGTTATCCCCCTAcaatcaat          <  3:202526/141‑1 (MQ=255)

                                                                                                                                  gAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAGATTGTGAAGTTAAATTTGCAGAAGGTTATCCCCCTAcaatcaat          >  2:676146/1‑141 (MQ=255)

                                                                                                                                    aGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAGGCTTCTGTTTAATTGAGATTGTGTAGTTAAATTTGCAGAAGGTTATCCCCCTACAATCAATAg        >  1:758322/1‑141 (MQ=255)

                                                                                                                                      gTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAGATTGTGAAGTTAAATTTGCAGAAGGTTATCCCCCTACAATCAATAGTc      <  4:338952/141‑1 (MQ=255)

                                                                                                                                       tACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAGATTGTGAAGTTAAATTTGCAGAAGGTTATCCCCCTACAATCAATAGTcc     <  1:6384/141‑1 (MQ=255)

                                                                                                                                        aCTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAGATTGTGAAGTTAAATTTGCAGAAGTTTATCCCCCTACAATCAATAGTCCg    <  4:321178/141‑1 (MQ=255)

                                                                                                                                        aCTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAGATTGTGAAGTTAAATTTGCAGAAGGTTATCCCCCTACAATCAATAGTCCg    >  4:5370/1‑141 (MQ=255)

                                                                                                                                          tATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAGATTGTGAAGTTAAATTTGCAGAAGGTTATCCCCCTACAATCAATAGTCCGaa  >  1:278777/1‑141 (MQ=255)

                                                                                                                                          tATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAGATTGTGAAGTTAAATTTGCAGAAGGTTATCCCCCTACAATCAATAGTCCGaa  >  2:312639/1‑141 (MQ=255)

ACAACATGTATTAACTCAAATATCACAAATTCAACAATTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACAATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAGATTGTGAAGTTAAATTTGCAGAAGGTTATCCCCCTACAATCAATAGTCCGAA  >  USA300TCH1516_ALE/1436117‑1436395

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`