| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 1,451,876 | T→C | 5.9% | A682A (GCA→GCG) | odhA ← | 2‑oxoglutarate dehydrogenase E1 component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 1,451,876 | 0 | T | C | 5.9% | 356.6 / 8.0 | 117 | A682A (GCA→GCG) | odhA | 2‑oxoglutarate dehydrogenase E1 component |
| Reads supporting (aligned to +/- strand): ref base T (50/60); new base C (3/4); total (53/64) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.91e-01 | |||||||||||
Only 100 of 121 total aligned reads displayed.
GATGAATGTTCAGGCCCTTGACCCTCATATGCATGAGGTAAGAATAATGTTAATCCTGAACGTTCTCCCCATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTATTCAAAACCAACTACTGCTGCTTCTGAAAGCGGAGAATTGTGTATATCAAATGTCGCTTTTTGATCAGGAACATGATGTAAAGGTGTATATGT > USA300TCH1516_ALE/1451736‑1452016 | gatgaATGTTCAGGCCCTTGACCCTCATATGCATGAGGTAAGAATAATGTTAATCCTGAACGTTCTCCCCATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATtgt < 1:232752/141‑1 (MQ=255) tgaATGTTCAGGCCCTTGACCCTCATATGCATGAGGTAAGAATAATGTTAATCCTGAACGTTCTCCCCATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGc > 2:817841/1‑141 (MQ=255) tgaATGTTCAGGCCCTTGACCCTCATATGCATGAGGTAAGAATAATGTTAATCCTGAACGTTCTCCCCATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGc < 3:319374/141‑1 (MQ=255) aTGTTCAGGCCCTTGACCCTCATATGCATGAGGTAAGAATAATGTTAATCCTGAACGTTCTCCCCATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTc > 1:578904/1‑141 (MQ=255) ttCCGGCCCTTGACCCTCATATGCATGAGGTAAGAATAATGTTAATCCTGAACGTTCTCCCCATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCa < 2:266888/141‑1 (MQ=255) ttCAGGCCCTTGACCCTCATATGCATGAGGTAAGAATAATGTTAATCCTGAACGTTCTCCCCATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCa < 2:175152/141‑1 (MQ=255) ttCAGGCCCTTGACCCTCATATGCATGAGGTAAGAATAATGTTAATCCTGAACGTTCTCCCCATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCa > 3:394980/1‑141 (MQ=255) ttCAGGCACTTGACCCTCATATGCCTGCGGTACGAATACTGTTCCACCTGAACGTTCTCCCCCTTTTGAGCGAGAAATGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCAACATATTGTCCTTccac > 2:914742/1‑139 (MQ=255) tCAGGCCCTTGACCCTCATATGCATGAGGTAAGAATAATGTTAATCCTGAACGTTCTCCCCATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCaa > 4:66321/1‑141 (MQ=255) tCAGGCCCTTGACCCTCATATGCATGAGGTAAGAATAATGTTAATCCTGAACGTTCTCCCCATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCaa > 4:220653/1‑141 (MQ=255) ggCCCTTGACCCTCATATGCAAGAGGTAAGAATAATGTTAATCCTGAACGTTCTCCCCATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAata > 2:465086/1‑141 (MQ=255) ccTTGACCCTCATATGCATGAGGTAAGAATAATGTTAATCCTGAACGTTCTCCCCATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTg > 2:739953/1‑141 (MQ=255) cTTGACCCTCATATGCATGAGGTAAGAATAATGTTAATCCTGAACGTTCTCCCCATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGa < 2:149084/141‑1 (MQ=255) cTTGACCCTCATATGCATGAGGTAAGAATAATGTTAATCCTGAACGTTCTCCCCATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGa > 1:822158/1‑141 (MQ=255) gACCCTCATATGCATGAGGTAAGAATAATGTTAATCCTGAACGTTCTCCCCATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGc < 1:109870/141‑1 (MQ=255) gACCCTCATATGCATGAGGTAAGAATAATGTTAATCCTGAACGTTCTCCCCATTTTGAGCGAGAACTGAATAAGAAGTTAACAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGc < 2:689680/141‑1 (MQ=255) aCCCTCATATGCATGAGGTAAGAATAATGTTAATCCTGAACGTTCTCCCCATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCt < 1:280337/141‑1 (MQ=255) cccTCATATGCAAGAGGTAAGAATAATGTTAATCCTGTACGTTCTCCCGTTTTTGAGCGAGAACTGAATAAGAAGTTGTTAAAAAAAATTTGTGACATATTTGCAAAATCACCATATTGTGTGTTCAAAATATTGAAGCtt > 4:100635/1‑141 (MQ=255) ccTCATATGCATGAGGTAAGAATAATGTTAATCCTGAACGTTCTCCCCATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCttt > 1:369945/1‑141 (MQ=255) acTCATATGCATGAGGTAAGAATAATGTTAATCCTGAACGTTCTCCCCATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCttt > 1:1030758/2‑141 (MQ=255) cTCATATGCATGAGGTAAGAATAATGTTAATCCTGAACGTTCTCCCCATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCtttt < 3:6864/141‑1 (MQ=255) tCATATGCATGAGGTAAGAATAATGTTAATCCTGAACGTTCTCCCCATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCttttt > 4:87245/1‑141 (MQ=255) ttatGCATGATGTAAGAATAATGTTAATCCTGAACGTTCTCCCCATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCttttttt < 4:371784/140‑1 (MQ=255) tgcataTGAGGTAAGAATAATGTTAATCCTGAACGTTCTCCCCATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTg > 2:988288/6‑141 (MQ=255) tatGCATGAGGTAAGAATAATGTTAATCCTGAACGTTCTCCCCATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTg < 3:144227/141‑1 (MQ=255) gCATGAGGTAAGAATAATGTTAATCCTGAACGTTCTCCCCATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGttt > 3:124855/1‑141 (MQ=255) cATGAGGTAAGAATAATGTTAATCCTGAACGTTCTCCCCATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGtttt < 2:1070122/141‑1 (MQ=255) cATGAGGTAAGAATAATGTTAATCCTGAACGTTCTCCCCATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGtttt > 2:1059746/1‑141 (MQ=255) aTGAGGTAAGAATAATGTTAATCCTGAACGTTCTCCCCATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTc < 4:162935/141‑1 (MQ=255) aTGAGGTAAGAATAATGTTAATCCTGAACGTTCTCCCCATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTc < 2:369945/141‑1 (MQ=255) tGAGGTAAGAATAATGTTAATCCTGAACGTTCTCCCCATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTcc < 3:450079/141‑1 (MQ=255) tGAGGTAAGAATAATGTTAATCCTGAACGTTCTCCCCATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTcc > 2:345533/1‑141 (MQ=255) aGGTAAGCATAATGTTAATACTGAACGTTCTCCCCAGTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATACTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCcac < 2:376707/141‑1 (MQ=255) aGGTAAGAATAATGTTAATCCTGAACGTTCTCCCCATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCcac < 4:390487/141‑1 (MQ=255) aGAATAATGTTAATCCTGAACGTTCTCCCCATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATtat > 3:266388/1‑141 (MQ=255) aGAATAATGTTAATCCTGAACGTTCTCCCCATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATtat > 1:7021/1‑141 (MQ=255) gAATAATGTTAATCCTGAACGTTCTCCCCATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTTTTTTCCACATtata > 2:735077/1‑141 (MQ=255) gAATAATGTTAATCCTGAACGTTCTCCCCATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATtata < 2:993363/141‑1 (MQ=255) gAATAATGTTAATCCTGAACGTTCTCCCCATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCAAAATATTGAAGCTTTTTTTGTTTTCCACATtata < 2:168557/141‑1 (MQ=255) ataatGTTAATCCTGAACGTTCTCCCCATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGc > 2:405752/1‑141 (MQ=255) aatGTTAATCCTGAACGTTCTCCCCATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCg < 2:924133/141‑1 (MQ=255) tGTTAATCCTGAACGTTCTCCCCATGTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGCGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTa < 2:190634/141‑1 (MQ=255) gTTAATCCTGAACGTTCTCCCCATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTAt > 4:203176/1‑141 (MQ=255) ttAATCCTGAACGTTCTCCCCATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTAtt < 1:306545/141‑1 (MQ=255) ttAATCCTGAACGTTCTCCCCATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTAtt < 1:645347/141‑1 (MQ=255) aaTCCTGAACGTTCTCCCCATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTATTCa > 1:352686/1‑141 (MQ=255) aaTCCTGAACGTTCTCCCCATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTATTCa > 3:399332/1‑141 (MQ=255) aTCCTGAACGTTCTCCCCATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGCGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTATTCaa > 4:324321/1‑141 (MQ=255) aTCCTGAACGTTCTCCCCATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGCGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTATTCaa > 4:323983/1‑141 (MQ=255) cTGAACGTTCTCCCCATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTATTCAAAAc < 1:863466/141‑1 (MQ=255) gTTCTCCCCATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACTTTATAGACGTATTCAAAACCAacta > 2:482480/1‑141 (MQ=255) gTTCTCCCCATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTATTCAAAACCAacta < 2:846904/141‑1 (MQ=255) ttCTCCCCATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTATTCAAAACCAactac < 1:449725/141‑1 (MQ=255) tctcCCCATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTATTCAAAACCAactcct > 1:97083/1‑141 (MQ=255) tcCCCATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTATTCAAAACCAACTActgc > 1:285403/1‑141 (MQ=255) cATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAAACACCATATTGTGCTTCCCAAATATTGAAGCTGTGTTTGTTTTCCACATTATAGCCGTATTCAAAACCATCTACTgctgct < 2:294715/141‑1 (MQ=255) aTTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTATTCAAAACCAACTACTGCTGCtt > 1:820719/1‑141 (MQ=255) aTTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTATTCAAAACCAACTACTGCTGCtt < 2:74195/141‑1 (MQ=255) aTTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATAGTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTATTCAAAACCAACTACTGCTGCtt < 1:1083049/141‑1 (MQ=255) tttGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTATTCAAAACCAACTACTGCTGCTTCt < 3:160859/141‑1 (MQ=255) tttGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTATTCAAAACCAACTACTGCTGCTTCt < 2:550038/141‑1 (MQ=255) aGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTATTCAAAACCAACTACTGCTGCTTCTGaaa > 1:981356/1‑141 (MQ=255) aGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCAAAATATTGAAGATTTTTTTGTTTTAAACATTATAGCAGTATTCAAAACCAACTACTGCTGCTTCTGaaa > 1:157815/1‑141 (MQ=255) gagaACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTATTCAAAACCAACTACTGCTGCTTCTGAAAGCg < 4:124855/141‑1 (MQ=255) gagaACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTATTCAAAACCAACTACTGCTGCTTCTGAAAGCg > 2:118692/1‑141 (MQ=255) agaACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTATTCAAAACCAACTACTGCTGCTTCTGAAAGCgg > 1:240027/1‑141 (MQ=255) gAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTATTCAAAACCAACTACTGCTGCTTCTGAAAGCGGAGAAtt < 4:73597/141‑1 (MQ=255) aTAAGAAGTTGTCAAAATTCATGTGTGACATATTTGCAAAATCACCATATTGCGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTATTCAAAACCAACTACTGCTGCTTCTGAAAGCGGAGAATtgt < 1:604220/141‑1 (MQ=255) tAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCCAATATTGAAGCTTTTTTTGTTTTTCACATTATAGCCGTATTCAAAACCACCTACTGCTCCTTCTCAAAGCGGAGAATtgtg > 1:662521/1‑141 (MQ=255) tAAGAAGTTGTCAAAAATCATTCGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTATTCAAAACCAACTACTGCTGCTTCTGAAAGCGGAGAATtgtg < 3:379727/141‑1 (MQ=255) gaagaagTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCAGTATTCAAAACCAACTACTGCTGCTTCTGAAAGCGGAGAATggtg < 2:641227/140‑1 (MQ=255) agaagTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTATTCAAAACCAACTACTGCTGCTTCTGAAAGCGGAGAATTGTGta < 3:74055/141‑1 (MQ=255) tGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTATTCAAAACCAACTACTGCTGCTTCTGAAAGCGGAGAATTGTGTATATCaa > 3:198625/1‑141 (MQ=255) gTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCTACATTATAGCCGTATTCAAAACCAACTACTGCTGCTTCTGAAAGCGGAGAATTGTGTATATCaaa > 2:631704/1‑141 (MQ=255) tCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTATTCAAAACCAACTACTGCTGCTTCTGAAAGCGGAGAATTGTGTATATCAAAt < 1:823371/141‑1 (MQ=255) tCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTATTCAAAACCAACTACTGCTGCTTCTGAAAGCGGAGAATTGTGTATATCAAAt < 2:293019/141‑1 (MQ=255) cAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTATTCAAAACCAACTACTGCTGCTTCTGAAAGCGGAGAATTGTGTATATCAAATg < 3:310588/141‑1 (MQ=255) cAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTATTCAAAACCAACTACTGCTGCTTCTGAAAGCGGAGAATTGTGTATATCAAATg < 1:386958/141‑1 (MQ=255) aaaaaTCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTATTCAAAACCAACTACTGCTGCTTCTGAAAGCGGAGAATTGTGTATATCAAATGt > 1:35739/1‑141 (MQ=255) aaaaaTCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTATTCAAAACCAACTACTGCTGCTTCTGAAAGCGGAGAATTGTGTATATCAAATGt < 2:822158/141‑1 (MQ=255) tCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTATTCAAAACCAACTACTGCTGCTTCTGAAAGCGGAGAATTGTGTATATCAAATGTCGCtt < 2:747472/141‑1 (MQ=255) cATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCACATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTATTCAAAACCAACTACTGCTGCTTCTGAAAGCGGAGAATTGTGTATATCAAATGTCGCttt < 2:228394/141‑1 (MQ=255) aTTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTATTCAAAACCAACTACTGCTGCTTCTGAAAGCGGAGAATTGTGTATATCAAATGTCGCtttt < 1:218753/141‑1 (MQ=255) tgtgACATATTTGCAAAATCACCATATTGCGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTATTCAAAACCAACTACTGCTGCTTCTGAAAGCGGAGAATTGTGTATATCAAATGTCGCTTTTTGa < 3:323983/141‑1 (MQ=255) tgtgACATATTTGCAAAATCACCATATTGCGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTATTCAAAACCAACTACTGCTGCTTCTGAAAGCGGAGAATTGTGTATATCAAATGTCGCTTTTTGa < 3:324321/141‑1 (MQ=255) tgACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTATTCAAAACCAACTACTGCTGCTTCTGAAAGCGGAGAATTGTGTATATCAAATGTCGCTTTTTGATc < 2:981320/141‑1 (MQ=255) gACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTATTCAAAACCAACTACTGCTGCTTCTGAAAGCGGAGAATTGTGTATATCAAATGTCGCTTTTTGATCa < 3:407914/141‑1 (MQ=255) aCATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTATTCAAAACCAACTACTGCTGCTTCTGAAAGCGGAGAATTGTGTATATCAAATGTCGCTTTTTGATCAg < 3:207827/141‑1 (MQ=255) aCATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTATTCAAAACCAACTACTGCTGCTTCTGAAAGCGGAGAATTGTGTATATCAAATGTCGCTTTTTGATCAg < 2:362467/141‑1 (MQ=255) aCATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTATTCAAAACCAACTACTGCTGCTTCTGAAAGCGGAGAATTGTGTATATCAAATGTCGCTTTTTGATCAg < 3:64851/141‑1 (MQ=255) aCATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTATTCAAAACCAACTACTGCTGCTTCTGAAAGCGGAGAATTGTGTATATCAAATGTCGCTTTTTGATCAg < 4:75852/141‑1 (MQ=255) tGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTTTTTTCTACTTTATAGCCGTATTCAAAACCAACTACTGCTGCTTCTGAAAGCGGAGAATTTTGTATATCAAATGTCGCTTTTTGATCAGGAACatg > 2:642447/1‑141 (MQ=255) tGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTATTCAAAACCAACTACTGCTGCTTCTGAAAGCGGAGAATTGTGTATATCAAATGTCGCTTTTTGATCAGGAACatg > 3:6676/1‑141 (MQ=255) aaaTCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTTCACATTATAGCCGTATTCAAAACCAACTACTGCTGCTTCTGAAAGCGGAGAATTGTGTATATCAAATGTCGCTTTTTGATCAGGAACATGATGt > 2:884256/1‑141 (MQ=255) tCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTATTCAAAACCAACTACTGCTGCTTCTGAAAGCGGAGAATTGTGTATATCAAATGTCGCTTTTTGATCAGGAACATGATGTaaa > 1:761045/1‑141 (MQ=255) aCCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTATTCAAAACCAACTACTGCTGCTTCTGAAAGCGGAGAATTGTGTATATCAAATGTCGCTTTTTGATCAGGAACATGATGTAAAgg < 4:349005/141‑1 (MQ=255) atTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTATTCACCACCAACTACTGCTGCTTCTGAAAGCGGAGAATTGTGTATATCAAATGTCGCTTTTTGATCAGGAACATGATGTAAAGGTGtat < 1:613849/141‑1 (MQ=255) tgtgCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTATTCAAAACCAACTACTGCTGCTTCTGATATTGGAGAATTGTGTATATCAAATGTCGCTTTTTGATCAGGAACATGATGTAAAGGTGTatat > 1:19700/1‑141 (MQ=255) tgtgCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTATTCAAAACCAACTACTGCTGCTTCTGAAAGCGGAGAATTGTGTATATCAAATGTCGCTTTTTGATCAGGAACATGATGTAAAGGTGTatat < 1:412764/141‑1 (MQ=255) tgCTTCCCAAATATTGAAGCTTTTTTTTTTTTTTTTTTTATAGCCGTATTCAAAACCAACTACTGCTGCTTCTGAAAGCGGAGAATTGTGTATATCAAATGTCGCTTTTTGATCAGGAACATGATTTAAAGGTGTATATGt > 2:108215/1‑141 (MQ=255) | GATGAATGTTCAGGCCCTTGACCCTCATATGCATGAGGTAAGAATAATGTTAATCCTGAACGTTCTCCCCATTTTGAGCGAGAACTGAATAAGAAGTTGTCAAAAATCATTTGTGACATATTTGCAAAATCACCATATTGTGCTTCCCAAATATTGAAGCTTTTTTTGTTTTCCACATTATAGCCGTATTCAAAACCAACTACTGCTGCTTCTGAAAGCGGAGAATTGTGTATATCAAATGTCGCTTTTTGATCAGGAACATGATGTAAAGGTGTATATGT > USA300TCH1516_ALE/1451736‑1452016 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |