Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 1,460,199 T→A 5.1% intergenic (‑231/+169) USA300HOU_RS07250 ← / ← USA300HOU_RS07255 hypothetical protein/putative CtpA‑like serine protease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,460,1990TA 5.1% 378.8 / 7.9 119intergenic (‑231/+169)USA300HOU_RS07250/USA300HOU_RS07255hypothetical protein/putative CtpA‑like serine protease
Reads supporting (aligned to +/- strand):  ref base T (52/60);  new base A (2/4);  total (54/65)
Fisher's exact test for biased strand distribution p-value = 6.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 120 total aligned reads displayed.

AATGAAAAATAGTAATTATCACACTATGGGATAAAAATAACCAATAAAAGTATCATATACCAATTAATTGATTGTTAAAACTAGTGAATAATACTATTTAAAAAGTATATGCTCATTGCATATTAAACCATGAATTGTTATCAGCGATGATAGAAATTGCGTTATTTACATCATTTGTAAATGTCTAATTTATATCTTTTAGAGTTAGACGTCAATAAAAATAAACACGCCTCCTAAATTGCTATCTTTGCAAATTTAGGAGGCGTGTCATTAACATA  >  USA300TCH1516_ALE/1460059‑1460336
                                                                                                                                            |                                                                                                                                         
aatgaaAAATAGTAATTATCACACTATGGGATAAAAATAACCAATAAAAGTATCATATACCAATTAATTGATTGTTAAAACTAGTGAATAATACTATTTAAAAAGTATATGCTCATTGCATATTAAACCATGAATTGTTAt                                                                                                                                           <  2:757715/141‑1 (MQ=255)
 atgaaAAATAGTAATTATCACACTATGGAAAAAAAATAACCAATAAAAGTATCATATACCAATTAATTGATTGTTAAAACTAGTGAATAATACTATTTAAAAAGTATATGCTCATTGCATATTAAACCATGAATTGTTATc                                                                                                                                          <  2:612649/141‑1 (MQ=255)
   gaaAAATAGTAATTATCACACTATGGGATAAAAATAACCAATAAAAGTATCATATACCAATTAATTGATTGTTAAAACTAGTGAATAATACTATTTAAAAAGTATATGCTCATTGCATATTAAACCATGAATTGTTATCAg                                                                                                                                        <  4:359100/141‑1 (MQ=255)
     aaaaTAGTAATTATCACACTATGGGATAAAAATAACCAATAAAAGTATCATATACCAATTAATTGATTGTTAAAACTAGTGAATAATACTATTTAAAAAGTATATGCTCATTGCATATTAAACCATGAATTGTTATCAGCg                                                                                                                                      <  3:153405/141‑1 (MQ=255)
      aaaTAGTAATTATCACACTATGGGATAAAAATAACCAATAAAAGTATCATATACCAATTAATTGATTGTTAAAACTAGTGAATAATACTATTTAAAAAGTATATGCTCATTGCATATTAAACCATGAATTGTTATCAGCga                                                                                                                                     <  1:637114/141‑1 (MQ=255)
      aaaTAGTAATTATCACACTATGGGATAAAAATAACCAATAAAAGTATCATATACCAATTAATTGATTGTTAAAACTAGTGAATAATACTATTTAAAAAGTATATGCTCATTGCATATTAAACCATGAATTGTTATCAGCga                                                                                                                                     >  2:901131/1‑141 (MQ=255)
      aaaTAGTAATTATCACACTATGGGATAAAAATAACCAATAAAAGTATCATATACCAATTAATTGATTGTTAAAACTAGTGAATAATACTATTTAAAAAGTATATGCTCATTGCATATTAAACCATGAATTGTTATCAGCga                                                                                                                                     >  3:241941/1‑141 (MQ=255)
      aaaTAGTAATTATCACACTATGGGATAAAAATAACCAATAAAAGTATCATATACCAATTAATTGATTGTTAAAACTAGTGAATAATACTATTTAAAAAGTATATGCTCATTGCATATTAAACCATGAATTGTTATCAGCga                                                                                                                                     <  1:316938/141‑1 (MQ=255)
        aTAGTAATTATCACACTATGGGATAAAAATAACCAATAAAAGTATCATATACCAATTAATTGATTGTTAAAACTAGTGAATAATACTATTTAAAAAGTATATGCTCATTGCATATTAAACCATGAATTGTTATCAGCgatg                                                                                                                                   >  1:745309/1‑141 (MQ=255)
          aGTAATTATCACACTATGGGATAAAAATAACCAATAAAAGTATCATATACCAATTAATTGATTGTTAAAACTAGTGAATAATACTATTTAAAAAGTATATGCTCATTGCATATTAAACCATGAATTGTTATCAGCgatgat                                                                                                                                 <  2:537746/141‑1 (MQ=255)
           gTAATTATCACACTATGGGATAAAAATAACCAATAAAAGTATCATATACCAATTAATTGATTGTTAAAACTAGTGAATAATACTATTTAAAAAGTATATGCTTCTTGCATATTAAACCATTAATTGTTATAATCGATGATa                                                                                                                                >  2:182066/1‑141 (MQ=255)
           gTAATTATCACACTATGGGATAAAAATAACCAATAAAAGTATCATATACCAATTAATTGATTGTTAAAACTAGTGAATAATACTATTTAAAAAGTATATGCTCATTGCATATTAAACCATGAATTGTTATCAGCGATGATa                                                                                                                                >  2:162738/1‑141 (MQ=255)
             aaTTATCACACTATGGGATAAAAATAACCAATAAAAGTATCATATACCAATTAATTGATTGTTAAAACTAGTGAATAATACTATTTAAAAAGTATATGCTCATTGCATATTAAACCATGAATTGTTATCAGCGATGATAGa                                                                                                                              <  1:739757/141‑1 (MQ=255)
              aTTATCACACTATGGGATAAAAATAACCAATAAAAGTATCATATACCAATTAATTGATTGTTAAAACTAGTGAATAATACTATTTAAAAAGTATATGCTCATTGCATATTAAACCATGAATTGTTATCAGCGATGATAGaa                                                                                                                             >  1:916584/1‑141 (MQ=255)
              aTTATCACACTATGGGATAAAAATAACCAATAAAAGTATCATATACCAATTAATTGATTGTTAAAACTAGTGAATAATACTATTTAAAAAGTATATGCTCATTGCATATTAAACCATGAATTGTTATCAGCGATGATAGaa                                                                                                                             >  1:539394/1‑141 (MQ=255)
                tATCACACTATGGGATAAAAATAACCAATAAAAGTATCATATACCAATTAATTGATTGTTAAAACTAGTGAATAATACTATTTAAAAAGTATATGCTCATTGCATATTAAACCATGAATTGTTATCAGCGATGATAGAAAt                                                                                                                           <  1:18422/141‑1 (MQ=255)
                 aTCACACTATGGGATAAAAATAACCAATAAAAGTATCATATACCAATTAATTGATTGTTAAAACTAGTGAATAATACTATTTAAAAAGTATATGCTCATTGCATATTAAACCATGAATTGTTATCAGCGATGATAGAAAtt                                                                                                                          >  4:179217/1‑141 (MQ=255)
                 aTCACACTATGGGATAAAAATAACCAATAAAAGTATCATATACCAATTAATTGATTGTTAAAACTAGTGAATAATACTATTTAAAAAGTATATGCTCATTGCATATTAAACCATGAATTGTTATCAGCGATGATAGAAAtt                                                                                                                          <  2:489975/141‑1 (MQ=255)
                     cacTATGGGATAAAAATAACCAATAAAAGTATCATATACCAATTAATTGATTGTTAAAACTAGTGAATAATACTATTTAAAAAGTATATGCTCATTGCATATTAAACCATGAATTGTTATCAGCGATGATAGAAATTGCGt                                                                                                                      >  2:494195/1‑141 (MQ=255)
                     cacTATGGGATAAAAATAACCAATAAAAGTATCATATACCAATTAATTGATTGTTAAAACTAGTGAATAATACTATTTAAAAAGTATATGCTCATTGCATATTAAACCATGAATTGTTATCAGCGATGATAGAAATTGCGt                                                                                                                      >  1:1069513/1‑141 (MQ=255)
                            ggATAAAAATAACCAATAAAAGTATCATATACCAATTAATTGATTGTTAAAACTAGTGAATAATACTATTTAAAAAGTATATGCTCATTGCATATTAAACCATGAATTGTTATCAGCGATGATAGAAATTGCGTTATTTag                                                                                                               >  2:39439/1‑140 (MQ=255)
                              aTAAAAATAACCAATAAAAGTATCATATACCAATTAATTGATTGTTAAAACTAGTGAATAATACTATTTAAAAAGTATATGCTCATTGCATATTAAACCATGAATTGTTATCAGCGATGATAGAAATTGCGTTATTTAcat                                                                                                             >  4:1869/1‑141 (MQ=255)
                              aTAAAAATAACCAATAAAAGTATCATATACCAATTAATTGATTGTTAAAACTAGTGAATAATACTATTTAAAAAGTATATGCTCATTGCATATTAAACCATGAATTGTTATCAGCGATGATAGAAATTGCGTTATTTAcat                                                                                                             <  2:1040214/141‑1 (MQ=255)
                                  aaaTAACCAATAAAAGTATCATATACCAATTAATTGATTGTTAAAACTAGTGAATAATACTATTTAAAAAGTATATGCTCATTGCATATTAAACCATGAATTGTTATCAGCGATGATAGAAATTGCGTTATTTACATCAtt                                                                                                         <  1:887485/141‑1 (MQ=255)
                                  aaaTAACCAATAAAAGTATCATATACCAATTAATTGATTGTTAAAACTAGTGAATAATACTATTTAAAAAGTATATGCTCATTGCATATTAAACCATGAATTGTTATCAGCGATGATAGAAATTGCGTTATTTACATCAtt                                                                                                         <  2:717415/141‑1 (MQ=255)
                                   aaTAACCAATAAAAGTATCATATACCAATTAATTGATTGTTAAAACTAGTGAATAATACTATTTAAAAAGTATATGCTCATTGCATATTAAACCATGAATTGTTATCAGCGATGATAGAAATTGCGTTATTTACATCAttt                                                                                                        >  2:395727/1‑141 (MQ=255)
                                   aaTAACCAATAAAAGTATCATATACCAATTAATTGATTGTTAAAACTAGTGAATAATACTATTTAAAAAGTATATGCTCATTGCATATTAAACCATGAATTGTTATCAGCGATGATAGAAATTGCGTTATTTACATCAttt                                                                                                        <  1:446812/141‑1 (MQ=255)
                                    aTAACCAATAAAAGTATCATATACCAATTAATTGATTGTTAAAACTAGTGAATAATACTATTTAAAAAGTATATGCTCATTGCATATTAAACCATGAATTGTTATCAGCGATGATAGAAATTGCGTTATTTAcaaatgatg                                                                                                       <  1:641018/141‑8 (MQ=255)
                                    aTAACCAATAAAAGTATCATATACCAATTAATTGATTGTTAAAACTAGTGAATAATACTATTTAAAAAGTATATGCTCATTGCATATTAAACCATGAATTGTTATCAGCGATGATAGAAATTGCGTTATTTACATCATTTg                                                                                                       <  1:824415/141‑1 (MQ=255)
                                      aaCCAATAAAAGTATCATATACCAATTAATTGATTGTTAAAACTAGTGAATAATACTATTTAAAAAGTATATGCTCATTGCATATTAAACCATGAATTGTTATCAGCGATGATAGAAATTGCGTTATTTACATCATTTGTa                                                                                                     >  1:909884/1‑141 (MQ=255)
                                       aCCAATAAAAGTATCATATACCAATTAATTGATTGTTAAAACTAGTGAATAATACTATTTAAAAAGTATATGCTCATTGCATATTAAACCATGAATTGTTATCAGCGATGATAGAAATTGCGTTATTTACATCATTTGTaa                                                                                                    <  2:839912/141‑1 (MQ=255)
                                       aCCAATAAAAGTATCATATACCAATTAATTGATTGTTAAAACTAGTGAATAATACTATTTAAAAAGTATATGCTCATTGCATATTAAACCATGAATTGTTATCAGCGATGATAGAAATTGCGTTATTTACATCATTTGTaa                                                                                                    <  4:400840/141‑1 (MQ=255)
                                               aaGTATCATATACCAATTAATGGATTGTTAAAACTAGTGAATAATACTATTTAAAAAGTATTTGCTCATGGCATAGTAAACCAGGAATTGGTATGAGCGATTATAGAAATTGCGGTATTTACATCATTTGGAAATGTCGaa                                                                                            >  3:456195/1‑141 (MQ=255)
                                                 gTATCATATACCAATTAATTGATTGTTAAAACTAGTGAATAATACTATTTAAAAAGTATATGCTCATTGCATATTAAACCATGAATTGTTATCAGCGATGATAGAAATTGCGTTATTTACATCATTTGTAAATGTCTAAtt                                                                                          <  1:233632/141‑1 (MQ=255)
                                                  tATCATANACCAATTAATTGATTGTTAAAACTAGTGAATAATACTATTTAAAAAGTATATGCTCATTGCATATTAAACCATGAATTGTTACCAGCGATGATAGAAATTGCGTTATTTACATCATTTGTAAATGTCCAAttt                                                                                         <  2:813570/141‑1 (MQ=255)
                                                   aTCATATACCAATTAATTGATTGTTAAAACTAGTGAATAATACTATTTAAAAAGTATATGCTCATTGCATATTAAACCATGAATTGTTATCAGCGATGATAGAAATTGCGTTATTTACATCATTTGTAAATGTCTAATTta                                                                                        <  4:241941/141‑1 (MQ=255)
                                                    tCATATACCAATTAATTGATTGTTAAAACTAGTGAATAATACTATTTAAAAAGTATATGCTCATTGCATATTAAACCATGAATTGTTATCAGCGATGATAGAAATTGCGTTATTTACATCATTTGTAAATGTCTAATTtat                                                                                       >  4:235763/1‑141 (MQ=255)
                                                     cATATACCAATTAATTGATTGTTAAAACTAGTGAATAATACTATTTAAAAAGTATATGCTCATTGCATATTAAACCATGAATTGTTATCAGCGATGATAGAAATTGCGTTATTTACATCATTTGTAAATGTCTAATTtata                                                                                      <  1:416762/141‑1 (MQ=255)
                                                     cATATACCAATTAATTGATTGTTAAAACTAGTGAATAATACTATTTAAAAAGTATATGCTCATTGCATATTAAACCATGAATTGTTATCAGCGATGATAGAAATTGCGTTATTTACATCATTTGTAAATGTCTAATTtata                                                                                      <  2:472238/141‑1 (MQ=255)
                                                         taCCAATTAATTGATTGTTAAAACTAGTGAATAATACTATTTAAAAAGTATATGCTCATTGCATATTAAACCATGAATTGTTATCAGCGATGATAGAAATTGCGTTATTTACATCATTTGTAAATGTCTAATTTATATCtt                                                                                  <  2:744544/141‑1 (MQ=255)
                                                           ccAATTAATTGATTGTTAAAACTAGTGAATAATACTATTTAAAAAGTATATGCTCATTGCATATTAAACCATTAATTGTTATCAGCGATGATAGAAATTGCGTTATTTACATCATTTGTAAATGTCTAATTTATATCtttt                                                                                <  2:207379/141‑1 (MQ=255)
                                                            cAATTAATTGATTGTTAAAACTAGTGAATAATACTATTTAAAAAGTATATGCTCATTGCATATTAAACCATGAATTGTTATCAGCGATGATAGAAATTGCGTTATTTACATCATTTGTAAATGTCTAATTTATATCttttt                                                                               >  2:591676/1‑140 (MQ=255)
                                                            cAATTAATTGATTGTTAAAACTAGTGAATAATACTATTTAAAAAGTATATGCTCATTGCATATTAAACCATGAATTGTTATCAGCGATGATAGAAATTGCGTTATTTACATCATTTGTAAATGTCTAATTTATATCTTTTa                                                                               >  4:72537/1‑141 (MQ=255)
                                                            cAATTAATTGATTGTTAAAACTAGTGAATAATACTATTTAAAAAGTATATGCTAATTGCATATTAAACCATGAATTGTTATCAGCGATGATAGAAATTGCGTTATTTAAATCATTTGTAAATGTCTAATTTATATCttttt                                                                               >  4:413938/1‑140 (MQ=255)
                                                              attaattGATTGTTAAAACTAGTGAATAATACTATTTAAAAAGTATATGCTCATTGCATATTAAACCATGAATTGTTATCAGCGATGATAGAAATTGCGTTATTTACATCATTTGTAAATGTCTAATTTATATCTTTTaga                                                                             <  3:49797/141‑1 (MQ=255)
                                                              attaattGATTGTTAAAACTAGTGAATAATACTATTTAAAAAGTATATGCTCATTGCATATTAAACCATGAATTGTTATCAGCGATGATAGAAATTGCGTTATTTACATCATTTGTAAATGTCTAATTTATATCTTTTaga                                                                             <  2:941561/141‑1 (MQ=255)
                                                                taattGATTGTTAAAACTAGTGAATAATACTATTTAAAAAGTATATGCTCATTGCATATTAAACCATGAATTGTTATCAGCGATGATAGAAATTGCGTTATTTACATCATTTGTAAATGTCTAATTTATATCTTTTAGAGt                                                                           <  1:1018359/141‑1 (MQ=255)
                                                                taattGATTGTTAAAACTAGTGAATAATACTATTTAAAAAGTATATGCTCATTGCATATTAAACCATGAATTGTTATCAGCGATGATAGAAATTGCGTTATTTACATCATTTGTAAATGTCTAATTTATATCTTTTAGAGt                                                                           <  4:150187/141‑1 (MQ=255)
                                                                    tgattgTTAAAACTAGTGAATAATACTATTTAAAAAGTATATGCTCATTGCATATTAAACCATGAATTGTTATCAGCGATGATAGAAATTGCGTTATTTACATCATTTGTAAATGTCTAATTTATATCTTTTAGAGTTAGa                                                                       >  1:42512/1‑141 (MQ=255)
                                                                     gattgTTAAAACTAGTGAATAATACTATTTAAAAAGTATATGCTCATTGCATATTAAACCATGAATTGTTATCAGCGATGATAGAAATTGCGTTATTTACATCATTTGTAAATGTCTAATTTATATCTTTTAGAGTTAGAc                                                                      >  2:611198/1‑141 (MQ=255)
                                                                       ttgTTAAAACTAGTGAATAATACTATTTAAAAAGTATATGCTCATTGCATATTAAACCATGAATTGTTATCAGCGATGATAGAAATTGCGTTATTTACATCATTTGTAAATGTCTAATTTATATCTTTTAGAGTTAGACGt                                                                    <  1:620712/141‑1 (MQ=255)
                                                                       ttgTTAAAACTAGTGAATAATACTATTTAAAAAGTATATGCTAATTGCATATTAAACCATGAATTGTTATCAGCGATGATAGAAATTGCGTTATTTAAATCATTTGTAAATGTCTTATTTATATAATTTTGAGTTAGaaga                                                                    >  2:472804/1‑138 (MQ=255)
                                                                        tgTTAAAACTAGTGAATAATACTATTTAAAAAGTATATGCTCATTGCATATTAAATCATGAATTGTTATCAGCGATGATAGAAATTGCGTTATTTACATCATTTGTAAATGTCTAATTTATATCTTTTAGAGTTAGACGTc                                                                   <  4:180375/141‑1 (MQ=255)
                                                                        tgTTAAAACTAGTGAATAATACTATTTAAAAAGTATATGCTCATTGCATATTAAACCATGAATTGTTATCAGCGATGATAGAAATTGCGTTATTTACATCATTTGTAAATGTCTAATTTATATCTTTTAGAGTTAGACGTc                                                                   <  2:347215/141‑1 (MQ=255)
                                                                        tgTTAAAACTAGTGAATAATACTATTTAAAAAGTATATGCTCATTGCATATTAAACCATGAATTGTTATCAGCGATGATAGAAATTGCGTTATTTACATCATTTGTAAATGTCTAATTTATATCTTTTAGAGTTAGACGTc                                                                   <  2:751397/141‑1 (MQ=255)
                                                                          ttAAAACTAGTGAATAATACTATTTAAAAAGTATATGCTCATTGCATATTAAACCATGAATTGTTATCAGCGATGATAGAAATTGCGTTATTTACATCATTTGTAAATGTCTAATTTATATCTTTTAGAGTTAGACGTCaa                                                                 >  2:141521/1‑141 (MQ=255)
                                                                            aaaaCTAGTGAATAATACTATTTAAAAAGTATATGCTCATTGCATATTAAACCATGAACTGTTATCAGCGATGATAGAAATTGCGTTATTTATATCTTTTGTAAATGTCTAATTTATATCTTTTAGAGTTAGACGTCaata                                                               >  3:316883/1‑141 (MQ=255)
                                                                               aCTAGTGAATAATACTATTTAAAAAGTATATGCTCATTGCATATTAAACCATGAATTGTTATCAGCGATGATAGAAATTGCGTTATTTACATCATTTGTAAATGTCTAATTTATATCTTTTAGAGTTAGACGTCaataaaa                                                            <  2:883800/141‑1 (MQ=255)
                                                                                   gTGAATAATACTATTTAAAAAGTATATGCTCATTGCATATTAAACCATGAATTGTTATCAGCGATGATAGAAATTGCGTTATTTACATCATTTGTAAATGTCTAATTTATATCTTTTAGAGTTAGACGTCaataaaaataa                                                        >  2:1003790/1‑141 (MQ=255)
                                                                                   gTGAATAATACTATTTAAAAAGTATATGCTCATTGCATATTAAACCATGAATTGTTATCAGCGATGATAGAAATTGCGTTATTTACATCATTTGTAAATGTCTAATTTATATCTTTTAGAGTTAGACGTCaataaaaataa                                                        <  1:569762/141‑1 (MQ=255)
                                                                                       ataataCTATTTAAAAAGTATATGCTCATTGCATATTAAACCATGAATTGTTATCAGCGATGATAGAAATTGCGTTATTTACATCATTTGTAAATGTCTAATTTATATCTTTTAGAGTTAGACGTCAATAAAAATAAacac                                                    >  1:4652/1‑141 (MQ=255)
                                                                                        taataCTATTTAAAAAGTATATGCTCATTGCATATTAAACCATGAATTGTTATCAGCGATGATAGAAATTGCGTTATTTACATCATTTGTAAATGTCTAATTTATATCTTTTAGAGTTAGACGTCAATAAAAATAAACACg                                                   >  2:1075763/1‑141 (MQ=255)
                                                                                        taataCTAGTGAAAAAGTATATGCTCATAGTATATTAAAGCATGAATTGTTAACAGCGATGATAGAAATTGCGTTATTTACATCATTTGTAAATGTCTAATTTATATCTTTTAGGGTTAGACGTCAATAAAAATAAACACg                                                   <  2:40076/141‑1 (MQ=255)
                                                                                         aataCTATTTAAAAAGTATATGCTCATTGCATATTAAACCATGAATTGTTATCAGCGATGATAGAAATTGCGTTATTTACATCATTTGTAAATGTCTAATTTATATCTTTTAGAGTTAGACGTCAATAAAAATAAACACGc                                                  <  3:87628/141‑1 (MQ=255)
                                                                                          ataCTATTTAAAAAGTATATGCTCATTGCATATTAAACCATGAATTGTTATCAGCGATGATAGAAATTGCGTTATTTACATCATTTGTAAATGTCTAATTTATATCTTTTAGAGTTAGACGTCAATAAAAATAAACACGcc                                                 >  1:979610/1‑141 (MQ=255)
                                                                                              tATTTAAAAAGTATATGCTCATTGCATATTAAACCATGAATTGTTATCAGCGATGATAGAAATTGCGTTATTTACATCATTTGTAAATGTCTAATTTATATCTTTTAGAGTTAGACGTCAATAAAAATAAACACGcctcct                                             <  2:511630/141‑1 (MQ=255)
                                                                                              tATTTAAAAAGTATATGCTCATTGCATATTAAACCATGAATTGTTATCAGCGATGATAGAAATTGCGTTATTTACATCATTTGTAAATGTCTAATTTATATCTTTTAGAGTTAGACGTCAATAAAAATAAACACGcctcct                                             <  4:408241/141‑1 (MQ=255)
                                                                                                 ttAAAAAGTATATGCTCATTGCATATTAAACCATGAATTGTTATCAGCGATGATAGAAATTGCGTTATTTACATCATTTGTAAATGTCTAATTTATATCTTTTAGAGTTAGACGTCAATAAAAATAAACACGCCTCCTaaa                                          <  2:592279/141‑1 (MQ=255)
                                                                                                   aaaaaGTATATGCTCATTGCATATTAAACCATGAATTGTTATCAGCGATGATAGAAATTGCGTTATTTACATCATTTGTAAATGTCTAATTTATATCTTTTAGAGTTAGACGTCAATAAAAATAAACACGCCTCCTAAAtt                                        <  3:39594/141‑1 (MQ=255)
                                                                                                   aaaaaGTATATGCTCATTGCATATTAAACCATGAATTGTTATCAGCGATGATAGAAATTGCGTTATTTACATCATTTGTAAATGTCTAATTTATATCTTTTAGAGTTAGACGTCAATAAAAATAAACACGCCTCCTAAAtt                                        >  1:31101/1‑141 (MQ=255)
                                                                                                   aaaaaGTATATGCTCATTGCATATTAAACCATGAATTGTTATCAGCGATGATAGAAATTGCGTTATTTACATCATTTGTAAATGTCTAATTTATATCTTTTAGAGTTAGACGTCAATAAAAATAAACACGCCTCCTAAAtt                                        <  1:733361/141‑1 (MQ=255)
                                                                                                    aaaaGTATATGCTCATTGCATATTAAACCATGAATTGTTATCAGCGATGATAGAAATTGCGTTATTTACATCATTTGTAAATGTCTAATTTATATCTTTTAGAGTTAGACGTCAATAAAAATAAACACGCCTCCTAAATTg                                       >  2:1049554/1‑141 (MQ=255)
                                                                                                       aGTATATGCTCATTGCATATTAAACCATGAATTGTTATCAGCGATGATAGAAATTGCGTTATTTACATCATTTGTAAATGTCTAATTTATATCTTTTAGAGTTAGACGTCAATAAAAATAAACACGCCTCCTAAATTGCTa                                    >  2:240218/1‑141 (MQ=255)
                                                                                                       aGTATATGCTCATTGCATATTAAACCATGAATTGTTATCAGCGATGATAGAAATTGCGTTATTTACATCATTTGTAAATGTCTAATTTATATCTTTTAGAGTTAGACGTCAATAAAAATAAACACGCCTCCTAAATTGCTa                                    >  2:240178/1‑141 (MQ=255)
                                                                                                       aGTATATGCTCATTGCATATTAAACCATGAATTGTTATCAGCGATGATAGAAATTGCGTTATTTACAGCATTTGTAAATGTCTAATTTATATCTTTTAGAGTTAGACGTCAATAAAAATAAACACGCCTCCTAAATTGCTa                                    >  1:214569/1‑141 (MQ=255)
                                                                                                           tatGCTCATTGCATATTAAACCATGAATTGTTATCAGCGATGATAGAAATTGCGTTATTTACATCATTTGTAAATGTCTAATTTATATCTTTTAGAGTTAGACGTCAATAAAAATAAACACGCCTCCTAAATTGCTATCtt                                <  1:168650/141‑1 (MQ=255)
                                                                                                            atGCTCATTGCATATTAAACCATGAATTGTTATCAGCGATGATAGAAATTGCGTTATTTACATCATTTGTAAATGTCTAATTTATATCTTTTAGAGTTAGACGTCAATAAAAATAAACACGCCTCCTAAATTGCTATAttt                               >  4:414139/1‑141 (MQ=255)
                                                                                                             tGCTCATTGCATATTAAACCATGAATTGTTATCAGCGATGATAGAAATTGCGTTATTTACATCATTTGTAAATGTCTAATTTATATCTTTTAGAGTTAGACGTCAATAAAAATAAACACGCCTCCTAAATTGCTATCTTTg                              >  2:974078/1‑141 (MQ=255)
                                                                                                                tCATTGCATATTAAACCATGAATTGTTATCAGCGATGATAGAAATTGCGTTATTTACATCATTTGTAAATGTCTAATTTATATCTTTTAGAGTTAGACGTCAATAAAAATAAACACGCCTCCTAAATTGCTATCTTTGCaa                           >  2:900754/1‑141 (MQ=255)
                                                                                                                    tGCATATTAAACCATGAATTGTTATCAGCGATGATAGAAATTGCGTTATTTACATCATTTGTAAATGTCTAATTTATATCTTTTAGAGTTAGACGTCAATAAAAATAAACACGCCTCCTAAATTGCTATCTTTGCAAAttt                       <  2:539394/141‑1 (MQ=255)
                                                                                                                       atatTAAACCATGAATTGTTATCAGCGATGATAGAAATTGCGTTATTTACATCATTTGTAAATGTCTAATTTATATCTTTTAGCGTTAGACGTCAATAAAAATAAACACGCCTCCTAAATTGCTATCTTTGCAAATTCagg                    >  2:225944/1‑141 (MQ=255)
                                                                                                                          ttAAACCATGAATTGTTATCAGCGATGATAGAAATTGCGTTATTTACATCATTTGTAAATGTCTAATTTATATCTTTTAGAGTTAGACGTCAATAAAAATAAACACGCCTCCTAAATTGCTATCTTTGCAAATTTaggagg                 <  4:101318/141‑1 (MQ=255)
                                                                                                                          ttAAACCATGAATTGTTATCAGCGATGATAGAAATTGCGTTATTTACATCATTTGTAAATGTCTAATTTATATCTTTTAGAGTTAGACGTCAATAAAAATAAACACGCCTCCTAAATTGCTATCTTTGCAAATTTaggagg                 <  2:274077/141‑1 (MQ=255)
                                                                                                                            aaaCCATGAATTGTTATCAGCGATGATAGAAATTGCGTTATTTACATCATTTGTAAATGTCTAATTTATATCTTTTAGAGTTAGACGTCAATAAAAATAAACACGCCTCCTAAATTGCTATCTTTGCAAATTTAGGAGGCg               >  1:292149/1‑141 (MQ=255)
                                                                                                                              agcATGAATTGTTAACAGCGATGATAGAAATTGCGTTATTTACATCATTTGTAAATGTCTAATTTATATCTTTTAGGGTTAGACGTCAATAAAAATAAACACGCCTCCTAAATTGCTATCTTTGCAAATTTAGGAAGCgtg             <  3:203873/139‑1 (MQ=255)
                                                                                                                              aCCATGAATTGTTATCAGCGATGATAGAAATTGCGTTATTTACATCATTTGTAAATGTCTAATTTATATCTTTTAGAGTTAGACGTCAATAAAAATAAACACGCCTCCTAAATTGCTATCTTTGCAAATTTAGGAGGCgtg             >  1:995365/1‑141 (MQ=255)
                                                                                                                                  tGAATTGTTATCAGCGATGATAGAAATTGCGTTATTTACATCATTTGTAAATGTCTAATTTATATCTTTTAGAGTTAGACGTCAATAAAAATAAACACGCCTCCTAAATTGCTATCTTTGCAAATTTAGGAGGCGTGTCAt         <  1:421756/141‑1 (MQ=255)
                                                                                                                                   gAATTGTTATCAGCGATGATAGAAATTGCGTTATTTACATCATTTGTAAATGTCTAATTTATATCTTTTAGAGTTAGACGTCAATAAAAATAAACACGCCTCCTAAATTGCTATCTTTGCAAATTTAGGAGGCGTGTCAtt        <  2:714819/141‑1 (MQ=255)
                                                                                                                                    aaTTGTTATCAGCGATGATAGAAATTGCGTTATTTACATCATTTGTAAATGTCTAATTTATATCTTTTAGAGTTAGACGTCAATAAAAATAAACACGCCTCCTAAATTGCTATCTTTGCAAATTTAGGAGGCGTGTCATTa       <  3:169292/141‑1 (MQ=255)
                                                                                                                                     aTTGTTAACAGCGATGATAGAAATTGCGTTATTTACATCATTTGTAAAt                                                                                                  <  3:365802/49‑1 (MQ=255)
                                                                                                                                     aTTGTTAACAGCGATGATAGAAATTGCGTTATTTACATCATTTGTAAAt                                                                                                  >  4:365802/1‑49 (MQ=255)
                                                                                                                                      ttGTTATCAGCGATGATAGAAATTGCGTTATTTCCATCATTTGTAAATGTCTCATTTCTATCTTTTAGAGTTAGACGTCAATAAAAATAAACACGCCTCCTAAATTGCTATCTTTGCACATTTAGGAGGCGCGTCATTAAc     >  2:321659/1‑141 (MQ=255)
                                                                                                                                      ttGTTATCAGCGATGATAGAAATTGCGTTATTTACATCATTTGTAAATGTCTAATTTATATCTTTTAGAGTTAGACGTCAATAAAAATAAACACGCCTCCTAAATTGCTATCTTTGCaa                           >  1:794887/1‑119 (MQ=255)
                                                                                                                                      ttGTTATCAGCGATGATAGAAATTGCGTTATTTACATCATTTGTAAATGTCTAATTTATATCTTTTAGAGTTAGACGTCAATAAAAATAAACACGCCTCCTAAATTGCTATCTTTGCaa                           <  2:794887/119‑1 (MQ=255)
                                                                                                                                       tGTTATCAGCGATGATAGAAATTGCGTTATTTACATCATTTGTAAATGTCTAATTTATATCTTTTAGAGTTAGACGTCAATAAAAATAAACACGCCTCCTAAATTGCTATCTTTGCAAATTTAGGAGGCGTGTCATTAACa    <  4:164846/141‑1 (MQ=255)
                                                                                                                                       tGTTATCAGCGATGATAGAAATTGCGTTATTTACATCATTTGTAAATGTCTAATTTATATCTTTTAGAGTTAGACGTCAATAAAAATAAACACGCCTCCTAAATTGCTATCTTTGCAAATTTAGGAGGCGTGTCATTAACa    <  1:1018539/141‑1 (MQ=255)
                                                                                                                                       tGTTATCAGCGATGATAGAAATTGCGTTATTTACATCATTTGTAAATGTCTAATTTATATCTTTTAGAGTTAGACGTCAATAAAAATAAACACGCCTCCTAAATTGCTATCTTTGCAAATTTAGGAGGCGTGTCATTAACa    <  1:994377/141‑1 (MQ=255)
                                                                                                                                        gTTATCAGCGATGATAGAAATGGCGTTATTTACATCATTTGTAAATGTCTAATTTATATCTTTTAGAGTTAGACGTCAATAAAAATAAACACGCCTCCTAAATTGCTATCTTTGCAAAGTTAGGAGGCGTGTCATTAACat   >  2:1083279/1‑141 (MQ=255)
                                                                                                                                         ttATCAGCGATGATAGAAATTGCGTTATTTACATCATTTGTAAATGTCTAATTTATATCTTTTAGAGTTAGACGTCAATAAAAATAAACACGCCTCCTAAATTGCTATCTTTGCAAATTTAGGAGGCGTGTCATTAACata  >  3:49119/1‑141 (MQ=255)
                                                                                                                                         ttATCAGCGATGATAGAAATTGCGTTATTTACATCATTTGTAAATGTCTAATTTATATCTTTTAGAGTTAGACGTCAATAAAAATAAACACGCCTCCTAAATTGCTATCTTTGCAAATTTAGGAGGCGTGTCATTAACata  >  1:944544/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                         
AATGAAAAATAGTAATTATCACACTATGGGATAAAAATAACCAATAAAAGTATCATATACCAATTAATTGATTGTTAAAACTAGTGAATAATACTATTTAAAAAGTATATGCTCATTGCATATTAAACCATGAATTGTTATCAGCGATGATAGAAATTGCGTTATTTACATCATTTGTAAATGTCTAATTTATATCTTTTAGAGTTAGACGTCAATAAAAATAAACACGCCTCCTAAATTGCTATCTTTGCAAATTTAGGAGGCGTGTCATTAACATA  >  USA300TCH1516_ALE/1460059‑1460336

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: