Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 1,481,732 C→T 5.1% T6764T (ACG→ACA ebh_1 ← Extracellular matrix‑binding protein ebh

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,481,7320CT 5.1% 492.0 / 8.7 154T6764T (ACG→ACAebh_1Extracellular matrix‑binding protein ebh
Reads supporting (aligned to +/- strand):  ref base C (72/74);  new base T (3/5);  total (75/79)
Fisher's exact test for biased strand distribution p-value = 7.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

Only 100 of 157 total aligned reads displayed.

CGTTCCATTGCTTGATCTAATTGTTCAGCTTTAGTTTTCACTTGATTTACTGTGCTAATATTTGGTGCATTTGTCACTTGCGTATTTAAATCTTGCTTTTGAGCATTATTTAAATGACTTAATTGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGCACCATTAAGCGCAGTTTTGTTAGTGCTCACTTGATTAGCTGCTTGTGTTACTGCAGATGGATCAAGTGTCGGCTGACTCGTTGCATTAATGATATTTTCAGCATTTG  >  USA300TCH1516_ALE/1481592‑1481871
                                                                                                                                            |                                                                                                                                           
cGTTCCATTGCTTGATCTAATTGTTCAGCTTTAGTTTTCACTTGATTTACTGTGCTAATATTTGGTGCATTTGTCACTTGCGTATTTAAATCTTGCTTTTGAGCATTATTTAAATGACTTAATTGGTTGATGTTAGCAGTc                                                                                                                                             >  1:1051564/1‑141 (MQ=255)
cGTTCCATTGCTTGATCTAATTGTTCAGCTTTAGTTTTCACTTGATTTACTGTGCTAATATTTGGTGCATTTGTCACTTGCGTATTTAAATCTTGCTTTTGAGCATTATTTAAATGACTTAATTGGTTGATGTTAGCAGTc                                                                                                                                             >  2:288236/1‑141 (MQ=255)
cGTTCCATTGCTTGATCTAATTGTTCAGCTTTAGTTTTCACTTGATTTACTGTGCTAATAATTGGTGCATTTGTCACTTGCTTATTTAAATCTTTCTTTTGAGCATTATTTAAATGACTTAATTGGTTGATGTTAGAAGTc                                                                                                                                             >  4:446318/1‑141 (MQ=255)
    ccATTGCTTGATCTAATTGTTCAGCTTTAGTTTTCACTTGATTTACTGTGCTAATATTTGGTGCATTTGTCACTTGTGTATTTAAATCTTGCTTTTGAGCATTGTTTAAATGACTTAATCGGTTGATGTTAGCAGTTGttt                                                                                                                                         >  2:85612/1‑141 (MQ=255)
    ccATTGCTTGATCTAATTGTTCAGCTTTAGTTTTCACTTGATTTACTGTGCTAATATTTGGTGCATTTGTCACTTGCGTATTTAAATCTTGCTTTTGAGCATTATTTAAATGACTTAATTGGTTGATGTTAGCAGTCGttt                                                                                                                                         <  1:168254/141‑1 (MQ=255)
           ttGATCTAATTGTTCAGCTTTAGTTTTCACTTGATTTACTGTGCTAATATTTGGTGCATTTGTCACTTGCGTATTTAAATCTTGCTTTTGAGCATTATTTAAATGACTTAATTGGTTGATGTTAGCAGTCGTTTCTTGCtt                                                                                                                                  >  3:314435/1‑141 (MQ=255)
            tGATCTAATTGTTCAGCTTTAGTTGTCACTTGATTTACTGTGCTAATATTTGGTGCATTTGTCACTTGCGTATTTAAATCTTGCTTTTGAGCGTTATTTAAATGACTTAATTGGTTGATGTTAGCAGTCGTTTCTTGCttt                                                                                                                                 <  2:932252/141‑1 (MQ=255)
              aTCTAATTGTTCAGCTTTAGTTTTCACTTGATTTACTGTGCTAATATTTGGTGCATTTGTCACTTGCGTATTTAAATCTTGCTTTTGAGCATTATTTAAATGACTTAATTGGTTGATGTTAGCAGTCGTTTCTTGCttttt                                                                                                                               <  1:1070982/141‑1 (MQ=255)
               tCTAATTGTTCAGCTTTAGTTTTCACTTGATTTACTGTGCTAATATTTGGTGCATTTGTCACTTGCGTATTTAAATCTTGCTTTTGAGCATTATTTAAATGACTTAATTGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTa                                                                                                                              >  2:331526/1‑141 (MQ=255)
                 cggtagTTCAGCTTTAGTTTTCACTTGATTTACTGTGCTAATATTTGGTGCATTTGTCACTTGCGTATTTAAATCTTGCTTTTGAGCATTATTTAAATGACTTAATTGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTAtt                                                                                                                            <  2:54687/136‑1 (MQ=255)
                  aaTTGTTCAGCTTTAGTTTTCACTTGATTTACTGTGCTAATATTTGGTGCATTTGTCACTTGCGTATTTAAATCTTGCTTTTGAGCATTATTTAAATGACTTAATTGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTATTc                                                                                                                           >  1:352444/1‑141 (MQ=255)
                   aTTGTTCAGCTTTAGTTTTCACTTGATTTACTGTGCTAATATTTGGTGCATTTGTCACTTGCGTATTTAAATCTTGCTTTTGAGCATTATTTAAATGACTTAATTGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTATTCg                                                                                                                          <  1:167042/141‑1 (MQ=255)
                    ttGTTCAGCTTTAGTTTTCACTTGATTTACTGTGCTAATATTTGGTGCATTTGTCACTTGCTTATTTAAATCTTGCTTTTGAGCATTATTTAAATGACTTAATTGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTATTCGc                                                                                                                         <  4:137447/141‑1 (MQ=255)
                    ttGTTCAGCTTTAGTTTTCACTTGATTTACTGTGCTAATATTTGGTGCATTTGTCACTTGCGTATTTAAATCTTGCTTTTGAGCATTATTTAAATGACTTAATTGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTATTCGc                                                                                                                         >  3:276815/1‑141 (MQ=255)
                        tCAGCTTTAGTTTTCACTTGATTTACTGTGCTAATATTTGGTGCATTTGTCACTTGCGTATTTAAATCTTGCTTTTGAGCATTATTTAAATGACTTAATTGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTATTCGCTAAg                                                                                                                     >  2:127170/1‑141 (MQ=255)
                         cAGCTTTAGTTTTCACTTGATTTACTGTGCTAATATTTGGTGCATTTGTCACTTGCGTATTTAAATCTTGCTTTTGAGCATTATTTAAATGACTTAATTGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTATTCGCTAAGt                                                                                                                    <  2:485598/141‑1 (MQ=255)
                         cAGCTTTAGTTTTCACTTGATTTACTGTGCTAATATTTGGTGCATTTGTCACTTGCGTATTTAAATCTTGCTTTTGAGCATTATTTAAATGACTTAATTGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTATTCGCTAAGt                                                                                                                    <  1:231039/141‑1 (MQ=255)
                          aGCTTTAGTTTTCACTTGATTTACTGTGCTAATATTTGGTGCATTTGTCACTTGTGTATTTAAATCTTGCTTTTGAGCATTGTTTAAATGACTTAATCGGTTGATGTTAGCAGTTGTTTCTTGCTTTTTATTTGCTAAGtt                                                                                                                   >  2:625324/1‑141 (MQ=255)
                            cTTTAGTTTTCACTTGATTTACTGTGCTAATATTTGGTGCATTTGTCACTTGTGTATTTAAATCTTGCTTTTGAGCATTGTTTAAATGACTTAATCGGTTGATGTTAGCAGTTGTTTCTTGCTTTTTATTTGCTAAGtttt                                                                                                                 >  1:1073762/1‑141 (MQ=255)
                                aGTTTTCACTTGATTTACTGTGCTAATATTTGGTGCATTTGTCACTTGCGTATTTAAATCTTGCTTTTGAGCATTATTTAAATGACTTAATTGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGc                                                                                                             >  2:946446/1‑141 (MQ=255)
                                 gTTTTCACTTGATTTACTGTGCTAATATTTGGTGCATTTGTCACTTGCGTATTTAAATCTTGCTTTTGAGCATTATTTAAATGACTTAATTGGTCGATGTTAGCAGTCGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGCa                                                                                                            >  1:284259/1‑141 (MQ=255)
                                    ttCACTTGATTTACTGTGCTAATATTTGGTGCATTTGTCACTTGCGTATTTAAATCTTGCTTTTGAGCATTATTTAAATGACTTAATTGGTTGATGTTAGCAGTTGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGCACCa                                                                                                         <  1:362282/141‑1 (MQ=255)
                                    ttCACTTGATTTACTGTGCTAATATTTGGTGCATTTGTCACTTGCGTATTTAAATCTTGCTTTTGAGCATTATTTAAATGACTTAATTGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGCACCa                                                                                                         <  1:388976/141‑1 (MQ=255)
                                       aCTTGATTTACTGTGCTAATATTTGGTGCATTTGTCACTTGCGTATTTAAATCTTGCTTTTGAGCATTATTTAAATGACTTAATTGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGCACCATTa                                                                                                      >  3:256014/1‑141 (MQ=255)
                                         ttGATTTACTGTGCTAATATTTGGTGCATTTGTCACTTGCGTATTTAAATCTTGCTTTTGAGCATTATTTAAATGACTTAATTGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGCACCATTAAg                                                                                                    >  2:6845/1‑141 (MQ=255)
                                            tttttCTTTGCTAATATTTGGTGCATTTGTCACTTGCGTATTTAAATCTTGCTTTTGAGCATTATTTAAATGACTTAATTGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGCACCATTAAgcgc                                                                                                 <  3:71506/140‑1 (MQ=255)
                                             tttACTGTGCTAATATTTGGTGCATTTGTCACTTGCGTATTTAAATCTTGCTTTTGAGCATTATTTAAATGACTTAATTGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGCACCATTAAGCGCa                                                                                                >  1:593936/1‑141 (MQ=255)
                                                aCTGTGCTAATATTTGGTGCATTTGTCACTTGCGTATTTAAATCTTGCTTTTGAGCATTATTTAAATGACTTAATTGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGCACCATTAAGCGCAGtt                                                                                             <  2:416045/141‑1 (MQ=255)
                                                 cTGTGCTAATATTTGGTGCATTTGTCACTTGCGTATTTAAATCTTGCTTTTGAGCATTATTTAAATGACTTAATTGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGCACCATTAAGCGCAGttt                                                                                            >  2:1008359/1‑141 (MQ=255)
                                                 cTGTGCTAATATTTGGTGCATTTGTCACTTGCGTATTTAAATCTTGCTTTTGAGCATTATTTAAATGACTTAATTGGTTGATGATAGCAGTCGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGCACCATTAAGCGCAGttt                                                                                            >  1:255444/1‑141 (MQ=255)
                                                  tgtgCTAATATTTGGTGCATTTGTCACTTGCGTATTTAAATCTTGCTTTTGAGCATTATTTAAATGACTTAATTGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGCACCATTAAGCGCAGtttt                                                                                           >  3:28510/1‑141 (MQ=255)
                                                    tgCTAATATTTGGTGCATTTGTCACTTGCGTATTTAAATCTTGCTTTTGAGCATTATTTAAATGACTTAATTGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGCACCATTAAGCGCAGTTTTGt                                                                                         >  1:636183/1‑141 (MQ=255)
                                                     gCTAATATTTGGTGCATTTGTCACTTGCGTATTTAAATCTTGCTTTTGAGCATTATTTAAATGACTTAATTGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGCACCATTAAGCGCAGTTTTGtt                                                                                        <  1:683191/141‑1 (MQ=255)
                                                         atatTTGGTGCATTTGTCACTTGCGTATTTAAATCTTGCTTTTGAGCATTATTTAAATGACTTAATTGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGCACCATTAAGCGCAGTTTTGTTAGTg                                                                                    <  3:117240/141‑1 (MQ=255)
                                                         atatTTGGTGCATTTGTCACTTGCGTATTTAAATCTTGCTTTTGAGCATTATTTAAATGACTTAATTGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGCACCATTAAGCGCAGTTTTGTTAGTg                                                                                    <  2:924208/141‑1 (MQ=255)
                                                          tatTTGGTGCATTTGTCACTTGCGTATTTAAATCTTGCTTTTGAGCATTATTTAAATGACTTAATTGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGCACCATTAAGCGCAGTTTTGTTAGTGc                                                                                   <  2:870615/141‑1 (MQ=255)
                                                               ggTGCATTTGTCACTTGCGTATTTAAATCTTGCTTTTGAGCATTATTTAAATGACTTAATTGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGCACCATTAAGCGCAGTTTTGTTAGTGCTCACt                                                                              <  1:160361/141‑1 (MQ=255)
                                                               ggTGCATTTGTCACTTGCGTATTTAAATCTTGCTTTTGAGCATTATTTAAATGACTTAATTGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTACTCGCTAAGTTTTGTGCACCATTAAGCGCAGTTTTGTTAGTGCTCACt                                                                              <  3:344578/141‑1 (MQ=255)
                                                                gTGCATTTGTCACTTGCGTATTTAAATCTTGCTTTTGAGCATTATTTAAATGACTTAATTGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGCACCATTAAGCGCAGTTTTGTTAGTGCTCACtt                                                                             >  4:13549/1‑141 (MQ=255)
                                                                gTGCATTTGTCACTTGCGTATTTAAATCTTGCTTTTGAGCATTATTTAAATGACTTAATTGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGCACCATTAAGCGCAGTTTTGTTAGTGCTCACtt                                                                             >  1:295111/1‑141 (MQ=255)
                                                                gTGCATTTGTCACTTGCGTATTTAAATCTTGCTTTTGAGCATTATTTAAATGACTTAATTGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGCACCATTAAGCGCAGTTTTGTTAGTGCTCACtt                                                                             >  4:346541/1‑141 (MQ=255)
                                                                   cATTTGTCACTTGCGTATTTAAATCTTGCTTTTGAGCATTATTTAAATGACTTAATTGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGCACCATTAAGCGCAGTTTTGTTAGTGCTCACTTGAt                                                                          <  1:172836/141‑1 (MQ=255)
                                                                   cATTTGTCACTTGCGTATTTAAATCTTGCTTTTGAGCATTATTTAAATGACTTAATTGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGCACCATTAAGCGCAGTTTTGTTAGTGCTCACTTGAt                                                                          <  1:1069238/141‑1 (MQ=255)
                                                                     tttGTCACTTGCGTATTTAAATCTTGCTTTTGAGCATTATTTAAATGACTTAATTGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGCACCATTAAGCGCAGTTTTGTTAGTGCTCACTTGATTa                                                                        <  1:755096/141‑1 (MQ=255)
                                                                     tttGTCACTTGCGTATTTAAATCTTGCTTTTGAGCATTATTTAAATGACTTAATTGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGCACCATTAAGCGCAGTTTTGTTAGTGCTCACTTGATTa                                                                        >  2:736943/1‑141 (MQ=255)
                                                                      ttGTCACTTGCGTATTTAAATCTTGCTTTTGAGCATTATTTAAATGACTTAATTGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGCACCATTAAGCGCAGTTTTGTTAGTGCTCACTTGATTAg                                                                       <  4:164327/141‑1 (MQ=255)
                                                                        gTCACTTGCGTATTTAAATCTTGCTTTTGAGCATTATTTAAATGACTTAATTGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGCACCATTAAGCGCAGTTTTGTTAGTGCTCACTTGATTAgct                                                                     <  2:72329/141‑1 (MQ=255)
                                                                         tCACTTGCGTATTTAAATCTTGCTTTTGAGCATTATTTAAATGACTTAATTGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGCACCATTAAGCGCAGTTTTGTTAGTGCTCACTTGATTAgctg                                                                    >  1:729620/1‑141 (MQ=255)
                                                                            cTTGCGTATTTAAATCTTGCTTTTGAGCATTATTTAAATGACTTAATTGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGCACCATTAAGCGCAGTTTTGTTAGTGCTCACTTGATTAGCTGCtt                                                                 <  3:421871/141‑1 (MQ=255)
                                                                            cTTGCGTATTTAAATCTTGCTTTTGAGCATTATTTAAATGACTTAATTGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGCACCATTAAGCGCAGTTTTGTTAGTGCTCACTTGATTAGCTGCtt                                                                 <  2:1082817/141‑1 (MQ=255)
                                                                             ttGCGTATTTAAATCTTGCTTTTGAGCATTATTTAAATGACTTAATTGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGCACCATTAAGCGCAGTTTTGTTAGTGCTCACTTGATTAGCTGCTtg                                                                <  2:357499/141‑1 (MQ=255)
                                                                             ttGCGTATTTAAATCTTGCTTTTGAGCATTATTTAAATGACTTAATTGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGCACCATTAAGCGCAGTTTTGTTAGTGCTCACTTGATTAGCTGCTtg                                                                <  1:477868/141‑1 (MQ=255)
                                                                               gCGTATTTAAATCTTGCTTTTGAGCATTATTTAAATGACTTAATTGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGCACCATTAAGCGCAGTTTTGTTAGTGCTCACTTGATTAGCTGCTtgtg                                                              >  1:353080/1‑141 (MQ=255)
                                                                                cGTATTTAAATCTTGCTTTTGAGCATTATTTAAATGACTTAATTGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGCACCATTAAGCGCAGTTTTGTTAGTGCTCACTTGATTAGCTGCTTgtgt                                                             <  2:444774/141‑1 (MQ=255)
                                                                                cGTATTTAAATCTTGCTTTTGAGCATTATTTAAATGACTTAATTGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGCACCATTAAGCGCAGTTTTGTTAGTGCTCACTTGATTAGCTGCTTgtgt                                                             <  4:57605/141‑1 (MQ=255)
                                                                                 gTATTTAAATCTTGCTTTTGAGCATTATTTAAATGACTTAATTGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGCACCATTAAGCGCAGTTTTGTTAGTGCTCACTTGATTAGCTGCTTGTGtt                                                            <  1:1017985/141‑1 (MQ=255)
                                                                                 gTATTTAAATCTTGCTTTTGAGCATTATTTAAATGACTTAATTGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGCACCATTAAGCGCAGTTTTGTTAGTGCTCACTTGATTAGCTGCTTGTGtt                                                            >  2:160576/1‑141 (MQ=255)
                                                                                 gTATTTAAATCTTGCTTTTGAGCATTATTTAAATGACTTAATTGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGCACCATTAAGCGCAGTTTTGTTAGTGCTCACTTGATTAGCTGCTTGTGtt                                                            >  2:429962/1‑141 (MQ=255)
                                                                                   aTTTAAATCTTGCTTTTTAGCATTATTTAAATGACTTAATTGGTTGATTTTAGCAGTCGTTTCTTTCTTTTTATTCGCTAAGTTTTGTGCACCATTAAGCGCAGTTTTGTTAGTGCTCACTTGATTAGCTGCTTGTGTTAc                                                          <  2:615117/141‑1 (MQ=255)
                                                                                   aTTTAAATCTTGCTTTTGAGCATTATTTAAATGACTTAATTGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGCACCATTAAGCGCAGTTTTGTTAGTGCTCACTTGATTAGCTGCTTGTGTTAc                                                          <  1:581319/141‑1 (MQ=255)
                                                                                     ttAAATCTTGCTTTTGAGCATTATTTAAATGACTTAATTGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGCACCATTAAGCGCAGTTTTGTTAGTGCTCACTTGATTAGCTGCTTGTGTTACTg                                                        <  2:441417/141‑1 (MQ=255)
                                                                                      tAAATCTTGCTTTTGAGCATTATTTAAATGACTTAATTGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGCACCATTAAGCGCAGTTTTGTTAGTGCTCACTTGATTAGCTGCTTGTGTTACTGc                                                       >  1:109900/1‑141 (MQ=255)
                                                                                          tCTTGCTTTTGAGCATTATTTAAATGACTTAATTGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGCACCATTAAGCGCAGTTTTGTTAGTGCTCACTTGATTAGCTGCTTGTGTTACTGCAGAt                                                   >  1:684764/1‑141 (MQ=255)
                                                                                           cTTGCTTTTGAGCATTATTTAAATGACTTAATTGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGCACCATTAAGCGCAGTTTTGTTAGTGCTCACTTGATTAGCTGCTTGTGTTACTGCAGATg                                                  >  1:276459/1‑141 (MQ=255)
                                                                                              gCTTTTGAGCATTATTTAAATGACTTAATTGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGCACCATTAAGCGCAGTTTTGTTAGTGCTCACTTGATTAGCTGCTTGTGTTACTGCAGATGGAt                                               <  1:846382/141‑1 (MQ=255)
                                                                                               cTTTTGAGGAGTATTTAAATGACTTAATTGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTATTCGCTAAGTGTTGTGCACCATTAAGCGCAGTTTTGTTAGTGCTCACTTGATTAGCTGCTTGTGTTACTGCAGATGGATc                                              <  4:247544/141‑1 (MQ=255)
                                                                                                ttttGAGCATTATTTAAATGACTTAATTGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGCACCATTAAGCGCAGTTTTGTTAGTGCTCACTTGATTAGCTGCTTGTGTTACTGCAGATGGATCa                                             <  2:344998/141‑1 (MQ=255)
                                                                                                ttttGAGCATTATTTAAATGACTTAATTGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGCACCATTAAGCGCAGTTTTGTTAGTGCTCACTTGATTAGCTGCTTGTGTTACTGCAGATGGATCa                                             <  2:344963/141‑1 (MQ=255)
                                                                                                ttttGAGCATTACTTAAATGACTTAATTGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGCACCATTAAGCGCAGTTTTGTTAGTGCTCACTTGATTAGCTGCTTGTGTTACTGCAGATGGATCa                                             <  4:366747/141‑1 (MQ=255)
                                                                                                 tttGAGCATTATTTAAATGACTTAATTGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGCACCATTAAGCGCAGTTTTGTTAGTGCTCACTTGATTAGCTGCTTGTGTTACTGCAGATGGATCaa                                            <  1:1017495/141‑1 (MQ=255)
                                                                                                 tttGAGCATTATTTAAATGACTTAATTGGTTGATGTTAGCAGTCGTGTCTTGCTTTTTATTCGCTAAGTTTTGTGCACCATTAAGCGCAGTTTTGTTAGTGCTCACTTGATTAGCTGCTTGTGTTACTGCAGATGGATCaa                                            <  1:964730/141‑1 (MQ=255)
                                                                                                   tGAGCATTATTTAAATGACTTAATTGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGCACCATTAAGCGCAGTTTTGTTAGTGCTCACTTGATTAGCTGCTTGTGTTACTGCAGATGGATCAAgt                                          <  3:193947/141‑1 (MQ=255)
                                                                                                    gAGCATTATTTAAATGACTTAATTGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGCACCATTAAGCGCAGTTTTGTTAGTGCTCACTTGATTAGCTGCTTGTGTTACTGCAGATGGATCCAgtt                                         >  4:402067/1‑140 (MQ=255)
                                                                                                     aGCATTATTTAAATGACTTAATTGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGCACCATTAAGCGCAGTTTTGTTAGTGCTCACTTGATTAGCTGCTTGTGTTACTGCAGATGGATCAAgtgt                                        >  2:58635/1‑141 (MQ=255)
                                                                                                     aGCATTATTTAAATGACTTAATTGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGCACCATTAAGCGCAGTTTTGTTAGTGCTCACTTGATTAGCTGCTTGTGTTACTGCAGATGGATCAAgtgt                                        >  3:367463/1‑141 (MQ=255)
                                                                                                     aGCATTATTTAAATGACTTAATTGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGCACCATTAAGCGCAGTTTTGTTAGTGCTCACTTGATTAGCTGCTTGTGTTACTGCAGATGGATCAAgtgt                                        >  2:125688/1‑141 (MQ=255)
                                                                                                       cATTGTTTAAATGACTTAATCGGTTGATGTTAGCAGTTGTTTCTTGCTTTTTATTTGCTAAGTTTTGCGCACCATTAAGCGCAGTTTTGTTAGTGTTCACTTGATTAGCTGCTTGTGTTACTGCAGATGGATCAAgtgttt                                      <  1:1031710/141‑3 (MQ=255)
                                                                                                         ttattTAAATGACTTAATTGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTTTTCGGTAAGATTTGTGCACCATCAAGCGCAGATTTCTTAGTGCCCACTTGATTAGCTGCTTGTGTTGTTGTAGATGGATCAAGTGTCGGc                                    >  2:163115/1‑141 (MQ=255)
                                                                                                         ttattTAAATGACTTAATTGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGCACCATTAAGCGCAGTTTTGTTAGTGCTCACTTGATTAGCTGCTTGTGTTACTGCAGATGGATCAAGTGTCGGc                                    >  2:664738/1‑141 (MQ=255)
                                                                                                         ttattTAAATGACTTAATTGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGCACCATTAAGCGCAGTTTTGTTAGTGCTCACTTGATTAGCTGCTTGTGTTACTGCAGATGGATCAAGTGTCGGc                                    >  4:113246/1‑141 (MQ=255)
                                                                                                          tgtttAAATGACTTAATCGGTTGATGTTAGCAGTTGTTTCTTGCTTTTTATTTGCTAAGTTTTGCGCACCATTAAGCGCAGTTTTGTTAGTGTTCACTTGATTAGCTGCTTGTGTTACTGCAGATGGATCAAGTGTTGGCt                                   <  2:774668/139‑1 (MQ=255)
                                                                                                           attTAAATGACTTAATTGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGCACCATTAAGCGCAGTTTTGTTAGTGCTCACTTGATTAGCTGCTTGTGTTACTGCAGATGGATCAAGTGTCGGCTg                                  >  1:795538/1‑141 (MQ=255)
                                                                                                              tAAATGACTTAATTGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGCACCATTAAGCGCAGTTTTGTTAGTGCTCACTTGATTAGCTGCTTGTGTTACTGCAGATGGATCAAGTGTCGGCTGACt                               >  2:818679/1‑141 (MQ=255)
                                                                                                                aaTGACTTAATTGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGCACCATTAAGCGCAGTTTTGTTAGTGCTCACTTGATTAGCTGCTTGTGTTACTGCAGATGGATCAAGTGTCGGCTGACTCg                             >  2:326146/1‑141 (MQ=255)
                                                                                                                 aTGACTTAATTGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGCACCATTAAGCGCAGTTTTGTTAGTGCTCACTTGATTAGCTGCTTGTGTTACTGCAGATGGATCAAGTGTCGGCTGACTCGt                            <  3:235658/141‑1 (MQ=255)
                                                                                                                     cTTAATTGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGCACCATTAAGCGCAGTTTTGTTAGTGCTCACTTGATTAGCTGCTTGTGTTACTGCAGATGGATCAAGTGTCGGCTGACTCGTTGCa                        >  2:181939/1‑141 (MQ=255)
                                                                                                                       tAATTGGTTGATGTTCGCAGTCGTTTCTTGCTTCTTATTCGCCACGTTTTGTGCAACAAAAAGAGCAGTTTTGTTCGCCCTCAATTGCTTAGCTGATTGTGTTACTGCAGATGGATCAAGTGTCGGCTGCCTCGTTGCAtt                      <  3:224261/141‑1 (MQ=255)
                                                                                                                        aaTTGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGCACCATTAAGCGCAGTTTTGTTAGTGCTCACTTGATTAGCTGCTTGTGTTACTGCAGATGGATCAAGTGTCGGCTGACTCGTTGCATTa                     >  2:36949/1‑141 (MQ=255)
                                                                                                                         aTTGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGCACCATTAAGCGCAGTTTTGTTAGTGCTCACTTGATTAGCTGCTTGTGTTACTGCAGATGGATCAAGTGTCGGCTGACTCGTTGCATTaa                    <  1:649525/141‑1 (MQ=255)
                                                                                                                           tGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGCACCATTAAGCGCAGTTTTGTTAGTGCTCACTTGATTAGCTGCTTGTGTTACTGCAGATGGATCAAGTGTCGGCTGACTCGTTGCATTAATg                  <  3:367444/141‑1 (MQ=255)
                                                                                                                           tGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGCACCATTAAGCGCAGTTTTGTTAGTGCTCACTTGATTAGCTGCTTGTGTTACTGCAGATGGATCAAGTGTCGGCTGACTCGTTGCATTAATg                  <  2:357081/141‑1 (MQ=255)
                                                                                                                            ggTTGATGTTAGCAGTCGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGCACCATTAAGCGCAGTTTTGTTAGTGCTCACTTGATTAGCTGCTTGTGTTACTGCAGATGGATCAAGTGTCGGCTGACTCGTTGCATTAATGa                 >  3:382223/1‑141 (MQ=255)
                                                                                                                            ggTTGATGTTAGCAGTCGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGCACCATTAAGCGCAGTTTTGTTAGTGCTCACTTGATTAGCTGCTTGTGTTACTGCAGATGGATCAAGTGTCGGCTGACTCGTTGCATTAATGa                 >  2:802725/1‑141 (MQ=255)
                                                                                                                             gTTGATGTTAGCAGTCGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGCACCATTAAGCGCAGTTTTGTTAGTGCTCACTTGATTAGCTGCTTGTGTTACTGCAGATGGATCAAGTGTCGGCTGACTCGTTGCATTAATGat                <  2:612091/141‑1 (MQ=255)
                                                                                                                               tGATGTTAGCAGTCGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGCACCATTAAGCGCAGTTTTGTTAGTGCTCACTTGATTAGCTGCTTGTGTTACTGCAGATGGATCAAGTGTCGGCTGACTCGTTGCATTAATGatat              >  1:976448/1‑141 (MQ=255)
                                                                                                                                 aTGTTAGCAGTCGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGCACGCTTAATCGCAACTTTCTTAGTGCTCACTTGATTAGCTGTTTGTGTTACTGCAGATGGATCAAGTGTCGGCTGACTCGTTCCATTATTGATAttt            >  4:202676/1‑141 (MQ=255)
                                                                                                                                 aTGTTAGCAGTCGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGCACCATTAAGCGCAGTTTTGTTAGTGCTCACTTGATTAGCTGCTTGTGTTACTGAAGATGGATCAAGTCTCGGCTGACTCGTTGCATTAATGATAttt            <  3:410139/141‑1 (MQ=255)
                                                                                                                                       gCAGTCGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGCACCATTAAGCGCAGTTTTGTTAGTGCTCACTTGATTAGCTGCTTGTGTTACTGCAGATGGATCAAGTGTCGGCTGACTCGTTGCATTAATGATATTTTCAGCa      >  2:594263/1‑141 (MQ=255)
                                                                                                                                        cAGTCGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGCACCATTAAGCGCAGTTTTGTTAGTGCTCACTTGATTAGCTGCTTGTGTTACTGCAGATGGATCAAGTGTCGGCTGACTCGTTGCATTAATGATATTTTCAGCAt     >  1:861574/1‑141 (MQ=255)
                                                                                                                                           tCGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGCACCATTAAGCGCAGTTTTGTTAGTGCTCACTTGATTAGCTGCTTGTGTTACTGCAGATGGATCAAGTGTCGGCTGACTCGTTGCATTAATGATATTTTCAGCATTTg  <  1:169201/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                           
CGTTCCATTGCTTGATCTAATTGTTCAGCTTTAGTTTTCACTTGATTTACTGTGCTAATATTTGGTGCATTTGTCACTTGCGTATTTAAATCTTGCTTTTGAGCATTATTTAAATGACTTAATTGGTTGATGTTAGCAGTCGTTTCTTGCTTTTTATTCGCTAAGTTTTGTGCACCATTAAGCGCAGTTTTGTTAGTGCTCACTTGATTAGCTGCTTGTGTTACTGCAGATGGATCAAGTGTCGGCTGACTCGTTGCATTAATGATATTTTCAGCATTTG  >  USA300TCH1516_ALE/1481592‑1481871

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: