Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 109,522 G→A 6.2% T196T (ACG→ACA)  plc → 1‑phosphatidylinositol phosphodiesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE109,5220GA 6.2% 467.6 / 10.9 151T196T (ACG→ACA) plc1‑phosphatidylinositol phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base G (73/67);  new base A (6/4);  total (80/71)
Fisher's exact test for biased strand distribution p-value = 7.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 157 total aligned reads displayed.

AAGACATTTGAAGAAATTTTTAGAGAATATTATTATAATAACCCGCAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTAAATTTAAAAGTACAAGATGAGTATAAAGATTACTATGATAAAAAAGTT  >  USA300TCH1516_ALE/109382‑109660
                                                                                                                                            |                                                                                                                                          
aaGACATTTGAAGAAATTTTTAGAGAATATTATTATAATAACCCGCAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGgtacg                                                                                                                                            <  2:423917/141‑1 (MQ=255)
 aGACATTTGAAGAAATTTTTAGAGAATATTATTATAATAACCCGCAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGgtacgt                                                                                                                                           >  3:128893/1‑141 (MQ=255)
  gACATTTGAAGAAATTTTTAGAGAATATTATTATAATAACCCGCAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGgtacgta                                                                                                                                          >  1:118239/1‑141 (MQ=255)
   aCATTTGAAGAAATTTTTAGAGAATATTATTATAATAACCCGCAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGgtacgtac                                                                                                                                         >  2:426102/1‑141 (MQ=255)
    ctctcgaagaaATATTTAGACAATAATATTATAATAACCCGCAATATCAGAATCTTTTTTACACAGGAAATAAAGCAAATCCTACGTAAAAAGAAACAAAAAGTAAAATTTACCTATTCAATAAATTGGGGGATATGTACa                                                                                                                                        <  4:333396/136‑1 (MQ=255)
    cATTTGAAGAAATTTTTAGAGAATATTATTATAATAACCCGCAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACa                                                                                                                                        <  1:277040/141‑1 (MQ=255)
     aTTTGAAGAAATTTTTAGAGAATATTATTATAATAACCCGCAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACAt                                                                                                                                       <  2:799808/141‑1 (MQ=255)
     aTTTGAAGAAATTTTTAGAGAATATTATTATAATAACCCGCAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACAt                                                                                                                                       <  1:627922/141‑1 (MQ=255)
        tGAAGAAATTTTTAGAGAATATTATTATAATAACCCGCAATATCAGAATCTTTTTTTTATATGTAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATaaa                                                                                                                                    >  4:55352/1‑141 (MQ=255)
              aaTTTTTAGAGAATATTATTATAATAACCCGCAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTgg                                                                                                                              <  1:97069/141‑1 (MQ=255)
              aaTTTTTAGAGAATATTATTATAATAACCCGCAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTgg                                                                                                                              >  1:192948/1‑141 (MQ=255)
              aaTTTTTAGAGAATATTATTATAATAACCCGCAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTgg                                                                                                                              >  1:552244/1‑141 (MQ=255)
               aTTTTTAGAGAATATTATTATAATAACCCGCAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGt                                                                                                                             >  1:440467/1‑141 (MQ=255)
               aTTTTTAGAGAATATTATTATAATAACCCGCAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGt                                                                                                                             <  4:242416/141‑1 (MQ=255)
                 ttttAGAGAATATTATTATAATAACCCGCAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTa                                                                                                                           >  3:189008/1‑141 (MQ=255)
                 ttttAGAGAATATTATTATAATAACCCGCAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTa                                                                                                                           <  2:909908/141‑1 (MQ=255)
                  tttAGAGAATATTATTATAATAACCCGCAATATCAGAATCTTTTTTACACATGAAGTAATTCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGTGTGTACTTACATAAAAATTgtgtat                                                                                                                          >  4:42419/1‑136 (MQ=255)
                  tttAGAGAATATTATTATAATAACCCGCAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTGTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTAt                                                                                                                          >  1:214458/1‑141 (MQ=255)
                   ttAGAGAATATTATTATAATAACCCGCAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATg                                                                                                                         >  4:335712/1‑141 (MQ=255)
                    tAGAGAATATTATTATAATAACCCGCAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATgg                                                                                                                        >  3:367080/1‑141 (MQ=255)
                    tAGAGAATATTATTATAATAACCCGCAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATgg                                                                                                                        <  3:216304/141‑1 (MQ=255)
                    tAGAGAATATTATTATAATAACCCGCAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGATACATACATAAAAAGTGGTTATgg                                                                                                                        <  4:240272/141‑1 (MQ=255)
                     agagAATATTATTATAATAACCCGCAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGt                                                                                                                       >  2:548778/1‑141 (MQ=255)
                        gaATATTATTATAATAACCCGCAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCt                                                                                                                    <  4:253276/141‑1 (MQ=255)
                         aaTATTATTATAATAACCCGCAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTg                                                                                                                   >  2:243976/1‑141 (MQ=255)
                          atatTATTATAATAACCCGCAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGa                                                                                                                  >  1:1067448/1‑141 (MQ=255)
                          atatTATTATAATAACCCGCAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGa                                                                                                                  >  2:711848/1‑141 (MQ=255)
                          atatTATTATAATAACCCGCAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACATACATAAAGAGTGTTTATGGTGCTGa                                                                                                                  >  2:407628/1‑141 (MQ=255)
                           tattattatAATAACCCGCAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACATACATAAAGAGTGGTTATGGTGCTGac                                                                                                                 <  1:994585/141‑1 (MQ=255)
                             ttattatAATAACCCGCAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACATACATAAAGAGTGGTTATGGTGCTGacac                                                                                                               >  1:573583/1‑141 (MQ=255)
                                 tataATAACCCGCAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCa                                                                                                           <  3:53944/141‑1 (MQ=255)
                                 aataataAACCGCAATACCAGAATCTTTTTTACACAGGAAGTAAAGCGAATCTTACTTTAAAAGAAACGAAAAGAAAAATTGTCCTATTTAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCa                                                                                                           <  4:138838/140‑1 (MQ=255)
                                  ataataACCCGCAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAg                                                                                                          <  2:340038/141‑1 (MQ=255)
                                  ataataACCCGCAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAg                                                                                                          <  1:50648/141‑1 (MQ=255)
                                    aataaCCCGCAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACATACATAAAGAGTGGTTATGGTGCTGACACGTCAGGt                                                                                                        >  3:430571/1‑141 (MQ=255)
                                      taaCCCGCAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTAt                                                                                                      <  2:1048285/141‑1 (MQ=255)
                                        aCCCGCAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTc                                                                                                    >  2:608338/1‑141 (MQ=255)
                                         cccGCAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCa                                                                                                   <  2:170530/141‑1 (MQ=255)
                                         cccGCAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCa                                                                                                   >  1:1049931/1‑141 (MQ=255)
                                         cccGCAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCa                                                                                                   >  1:257802/1‑141 (MQ=255)
                                            gCAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATg                                                                                                >  2:778141/1‑141 (MQ=255)
                                             cAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATgg                                                                                               <  3:10896/141‑1 (MQ=255)
                                             cAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATgg                                                                                               >  1:291208/1‑141 (MQ=255)
                                                 atCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAg                                                                                           <  1:101430/141‑1 (MQ=255)
                                                   cAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGAc                                                                                         >  2:911447/1‑141 (MQ=255)
                                                     gAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACaa                                                                                       >  2:96264/1‑141 (MQ=255)
                                                     gAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACaa                                                                                       >  2:200037/1‑141 (MQ=255)
                                                     gAATCTTTTTTACACAGGAAGTAAGGCGAATCCTACTTTAAAAGAGACGGAAGGTAAAATTGTCCTATTGAGTAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTTAGGTATTCAATGGTCAGACaa                                                                                       >  2:96300/1‑141 (MQ=255)
                                                          ttttttACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGa                                                                                  <  2:451082/141‑1 (MQ=255)
                                                            ttttACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACa                                                                                >  4:281407/1‑141 (MQ=255)
                                                            ttttACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACa                                                                                >  1:140081/1‑141 (MQ=255)
                                                              ttACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACAtt                                                                              >  2:554172/1‑141 (MQ=255)
                                                                  acaGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGaa                                                                          >  1:530973/1‑141 (MQ=255)
                                                                   caGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGaaa                                                                         >  1:767608/1‑141 (MQ=255)
                                                                    aGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACATACATAAAGAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAAc                                                                        <  4:174986/141‑1 (MQ=255)
                                                                         gTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGaaaa                                                                   <  1:965213/141‑1 (MQ=255)
                                                                             tGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATtaa                                                               >  2:668359/1‑141 (MQ=255)
                                                                               cGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATtaata                                                             >  4:46276/1‑141 (MQ=255)
                                                                                gAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATtaataa                                                            >  4:299159/1‑141 (MQ=255)
                                                                                   tCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATgg                                                         >  2:258542/1‑141 (MQ=255)
                                                                                   tCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATgg                                                         <  1:957070/141‑1 (MQ=255)
                                                                                        cTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCt                                                    <  3:281407/141‑1 (MQ=255)
                                                                                         tttAAAATAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCtt                                                   <  2:198213/141‑1 (MQ=255)
                                                                                          ttAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTa                                                  >  3:273252/1‑141 (MQ=255)
                                                                                          ttAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTa                                                  <  3:75142/141‑1 (MQ=255)
                                                                                          ttAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTa                                                  <  1:94824/141‑1 (MQ=255)
                                                                                          ttAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTa                                                  >  2:732781/1‑141 (MQ=255)
                                                                                          ttAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTa                                                  <  2:1069012/141‑1 (MQ=255)
                                                                                          ttAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTa                                                  <  1:1001551/141‑1 (MQ=255)
                                                                                            aaaaGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTaaa                                                <  2:825045/141‑1 (MQ=255)
                                                                                            aaaaGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTaaa                                                <  2:625424/141‑1 (MQ=255)
                                                                                              aaGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTAAAtt                                              >  4:166925/1‑141 (MQ=255)
                                                                                               aGAAACGAAAGTAAAAAGTGTTCTATTCAATAGAAAGGGGGGCAGGTACATAATAAGTGGTTATGGAATTGACAGGTAAGGTATTAAATAGGAAGACAAAACGACATTAAAAACGAAAATTAATAATGGTAGCTTaaaaat                                             >  2:314500/1‑138 (MQ=255)
                                                                                                   aCGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTAAATTTaaaa                                         >  4:126478/1‑141 (MQ=255)
                                                                                                    cGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTAAATTTAAAAg                                        >  1:728899/1‑141 (MQ=255)
                                                                                                     gAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTAAATTTAAAAGt                                       <  3:25322/141‑1 (MQ=255)
                                                                                                     gAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTAAATTTAAAAGt                                       >  2:17781/1‑141 (MQ=255)
                                                                                                     gAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTAAATTTAAAAGt                                       <  1:711848/141‑1 (MQ=255)
                                                                                                       aaGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTAAATTTAAAAGTAc                                     >  2:671410/1‑141 (MQ=255)
                                                                                                       aaGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTAAATTTAAAAGTAc                                     >  2:3733/1‑141 (MQ=255)
                                                                                                         ggTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTAAATTTAAAAGTACaa                                   <  3:210160/141‑1 (MQ=255)
                                                                                                          gTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTAAATTTAAAAGTACAAg                                  >  1:568763/1‑141 (MQ=255)
                                                                                                             aaaTTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTAAATTTAAAAGTACAAGATg                               >  1:358091/1‑141 (MQ=255)
                                                                                                               aTTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTAAATTTAAAAGTACAAGATGAg                             >  3:350669/1‑141 (MQ=255)
                                                                                                                    ccTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTAAATTTAAAAGTACAAGATGAGTATaa                        <  1:619381/141‑1 (MQ=255)
                                                                                                                              aGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTAAATTTAAAAGTACAAGATGAGTATAAAGATTACTAt              <  1:711740/141‑1 (MQ=255)
                                                                                                                              aGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTAAATTTAAAAGTACAAGATGAGTATAAAGATTACTAt              >  1:34944/1‑141 (MQ=255)
                                                                                                                              aGAATGGGGGGTACGCACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTAAATTTAAAAGTACAAGATGAGTATAAAGATTACTAt              <  1:903118/141‑1 (MQ=255)
                                                                                                                               gAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTAAATTTAAAAGTACAAGATGAGTATAAAGATTACTATg             >  1:616652/1‑141 (MQ=255)
                                                                                                                               gAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTAAATTTAAAAGTACAAGATGAGTATAAAGATTACTATg             >  2:130952/1‑141 (MQ=255)
                                                                                                                                aaTGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTAAATTTAAAAGTACAAGATGAGTATAAAGATTACTATGa            >  2:505340/1‑141 (MQ=255)
                                                                                                                                 aTGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTAAATTTAAAAGTACAAGATGAGTATAAAGATTACTATGAt           >  3:370594/1‑141 (MQ=255)
                                                                                                                                 aTGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTAAATTTAAAAGTACAAGATGAGTATAAAGATTACTATGAt           >  1:599357/1‑141 (MQ=255)
                                                                                                                                  tGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTAAATTTAAAAGTACAAGATGAGTATAAAGATTACTATGATa          <  2:530973/141‑1 (MQ=255)
                                                                                                                                    gggggTACGTACATATAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTAAATTTAAAAGTACAAGATGAGTATAAAGATTACTATGATaaa        <  1:902576/141‑1 (MQ=255)
                                                                                                                                    gggggTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGTGACATTTGAAACGAAAATTAATAATGGTAGCTTAAATTTAAAAGTACAAGATGAGTATAAAGATTACTATGATaaa        >  3:243423/1‑141 (MQ=255)
                                                                                                                                    gggggTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTAAATTTAAAAGTACAAGATGAGTATAAAGATTACTATGATaaa        >  2:640481/1‑141 (MQ=255)
                                                                                                                                    gggggTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTAAATTTAAAAGTACAAGATGAGTATAAAGATTACTATGATaaa        <  4:10876/141‑1 (MQ=255)
                                                                                                                                     ggggTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTAAATTTAAAAGTACAAGATGAGTATAAAGATTACTATGATaaaa       <  3:436222/141‑1 (MQ=255)
                                                                                                                                     ggggTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTAAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTAAATTTAAAAGTACAAGATGAGTATAAAGATAACAAAAATaaaa       <  2:331252/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                          
AAGACATTTGAAGAAATTTTTAGAGAATATTATTATAATAACCCGCAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTAAAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGTGGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAAATTAATAATGGTAGCTTAAATTTAAAAGTACAAGATGAGTATAAAGATTACTATGATAAAAAAGTT  >  USA300TCH1516_ALE/109382‑109660

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑