Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 1,526,104 C→T 5.2% S613S (TCG→TCA dinG_1 ← putative ATP‑dependent helicase DinG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,526,1040CT 5.2% 421.3 / 8.2 134S613S (TCG→TCAdinG_1putative ATP‑dependent helicase DinG
Reads supporting (aligned to +/- strand):  ref base C (53/73);  new base T (2/5);  total (55/79)
Fisher's exact test for biased strand distribution p-value = 6.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.40e-01

Only 100 of 140 total aligned reads displayed.

AAACGAATGATTAAATTTTAAAGTACCAGATATAAAAATGAGCGATTTAAATTTTTCCAATACTTGTTTCGTTAATACATCTTTTACAGCGTAATCTTTCACATATAAACGTATTGTTGATTTTTGAGATAAATTTTTAATCGAAATGAAACTAGTATGTCCAGCTTTTAAACTTTGTTCGATATTTTTAAATTTATCTTTTAAATACAACAACTGTTTCCGTAATGATTTAACTGTTTTATGACTAATGCCATTGAATATTTCTAGCGTTTTATTTAACTT  >  USA300TCH1516_ALE/1525963‑1526244
                                                                                                                                             |                                                                                                                                            
aaaCGAATGATTAAATTTTAAAGTACCAGATATAAAAATGAGCGATTTAAATTTTTCCAATACTTGTTTCGTTAATACATCTTTTACAGCGTAATCTTTCACATATAAACGTATTGTTGATTTTAAGATAAATTTTTAATc                                                                                                                                              >  1:884713/1‑141 (MQ=255)
 aaCGAATGATTAAATTTTAAAGTACCAGATATAAAAATGAGCGATTTAAATTTTTCCAATACTTGTTTCGTTAATACATCTTTTACAGCGTAATCTTTCACATATAAACGTATTGTTGATTTTTGAGATAAATTTTTAATc                                                                                                                                              >  2:186381/1‑141 (MQ=255)
 aaCGAATGATTAAATTTTAAAGTACCAGATATAAAAATGAGCGATTTAAATTTTTCCAATACTTGTTTCGTTAATACATCTTTTACAGCGTAATCTTTCACATATAAACGTATTGTTGATTTTTGAGATAAATTTTTAATc                                                                                                                                              >  3:430314/1‑141 (MQ=255)
    gAATGATTAAATTTTAAAGTACCAGATATAAAAATGAGCGATTTAAATTTTTCCAATACTTGTTTCGTTAATACATCTTTTACAGCGTAATCTTTCACATATAAACGTATTGTTGATTTTTGAGATAAATTTTTAATCGaa                                                                                                                                           <  2:328657/141‑1 (MQ=255)
      aTGATTAAATTTTAAAGTACCAGATATAAAAATGAGCGATTTAAATTTTTCCAATACTTGTTTCGTTAATACATCTTTTACAGCGTAATCTTTCACATATAAACGTATTGTTGATTTTTGAGATAAATTTTTAATCGAAAt                                                                                                                                         >  1:56272/1‑141 (MQ=255)
      aTGATTAAATTTTAAAGTACCAGATATAAAAATGAGCGATTTAAATTTTTCCAATACTTGTTTCGTTAATACATCTTTTACAGCGTAATCTTTCACATATAAACGTATTGTTGATTTTTGAGATAAATTTTTAATCGAAAt                                                                                                                                         >  1:914547/1‑141 (MQ=255)
      aTGATTAAATTTTAAAGTACCAGATATAAAAATGAGCGATTTAAATTTTTCCAATACTTGTTTCGTTAATACATCTTTTACAGCGTAATCTTTCACATATAAACGTATTGTTGATTTTTGAGATAAATTTTTAATCGAAAt                                                                                                                                         <  3:142256/141‑1 (MQ=255)
         aTTAAATTTTAAAGTACCAGATATAAAAATGAGCGATTTAAATTTTTCCAATACTTGTTTCGTTAATACATCTTTTACAGCGTAATCTTTCACATATAAACGTATTGTTGATTTTTGAGATAAATTTTTAATCGAAATGaa                                                                                                                                      <  1:34776/141‑1 (MQ=255)
             aaTTTTAAAGTACCAGATATAAAAATGAGCGATTTAAATTTTTCCAATACTTGTTTCGTTAATACATCTTTTACAGCGTAATCTTTCACATATAAACGTATTGTTGATTTTTGAGATAAATTTTTAATCGAAATGAAACTa                                                                                                                                  >  3:30704/1‑141 (MQ=255)
             aaTTTTAAAGTACCAGATATAAAAATGAGCGATTTAAATTTTTCCAATACTTGTTTCGTTAATACATCTTTTACAGCGTAATCTTTCACATATAAACGTATTGTTGATTTTTGAGATAAATTTTTAATCGAAATGAAACTa                                                                                                                                  >  1:845016/1‑141 (MQ=255)
                  tAAAGTACCAGATATAAAAATGAGCGATTTAAATTTTTTTAATATTTTTTTCGATAATACATCTTTTACAGCGTAATCTTTCACATATAAACGTATTCATGATTTTTGAGATAAATTTTTAATCGAAATGAAACTAGTATg                                                                                                                             >  4:100422/1‑141 (MQ=255)
                  tAAAGTACCAGATATAAAAATGAGCGATTTAAATTTTTCCAATACTTGTTTCGTTAATACATCTTTTACAGCGTAATCTTTCACATATAAACGTATTGTTGATTTTTCAGATAAATTTTTAATCGAAATGAAACTAGTATg                                                                                                                             >  4:305000/1‑141 (MQ=255)
                   aaaGTACCAGATATAAAAATGAGCGTTTTAAATTTTTCCAATACTTGTTTCGTTAATACATCTTTTACAGCGTAATCTTTCACATATAAACGTATTGTTGATTTTTGAGATAAATTTTTAATCGAAATGAAACTAGTATGt                                                                                                                            >  4:403950/1‑141 (MQ=255)
                   aaaGTACCAGATATAAAAATGAGCGATTTAAATTTTTCCAATACTTGTTTCGTTAATACATCTTTTACAGCGTAATCTTTCACATATAAACGTATTGTTGATTTTTGAGATAAATTTTTAATCGAAATGAAACTAGTATGt                                                                                                                            >  2:44629/1‑141 (MQ=255)
                   aaaGTACCAGATATAAAAATGAGCGATTTAAATTTTTCCAATACTTGTTTCGTTAATACATCTTTTACAGCGTAATCTTTCACATATAAACGTATTGTTGATTTTTGAGATAAATTTTTAATCGAAATGAAACTAGTATGt                                                                                                                            >  1:460959/1‑141 (MQ=255)
                   aaaGTACCACATATAAAAATGAGCGATTTAAATTTTTCCAATACTTGTTTCGTTAATACATCTTTTACAGCGTAATCTTTCACATATAAACGTATTGTTGATTTTTGAGATAAATTTTTAATCGAAATGAAACTAGTATGt                                                                                                                            >  1:955855/1‑141 (MQ=255)
                    aaGTACCAGATATAAAAATGAGCGATTTAAATTTTTCCAATACTTGTTTCGTTAATACATCTTTTCCAACGTAATCTTTCACATATAAACGTATTGTTGATTTTTGAGATAAATTTTTAATCGAAATGAAACTAGTATGTc                                                                                                                           >  3:100845/1‑141 (MQ=255)
                    aaGTACCAGATATAAAAATGAGCGATTTAAATTTTTCCAATACTTGTTTCGTTAATACATCTTTTACAGCGTAATCTTTCACATATAAACGTATTGTTGATTTTTGAGATAAATTTTTAATCGAAATGAAACTAGTATGTc                                                                                                                           <  1:746861/141‑1 (MQ=255)
                    aaGTACCAGATATAAAAATGAGCGATTTAAATTTTTCCAATACTTGTTTCGTTAATACATCTTTTACAGCGTAATCTTTCACATATAAACGTATTGTTGATTTTTGAGATAAATTTTTAATCGAAATGAAACTAGTATGTc                                                                                                                           >  1:694631/1‑141 (MQ=255)
                    aaGTACCAGATACGAATATGAGCGATTTAAGTTTTTCCAATACTTGTTGTATTAATACATCTTTTACAGCGTAATCTTTCACATATAAACGTATTGTTGATTTTTGAGATAAATTCTTAATAGAAATGAAACTAGTATGTc                                                                                                                           <  4:30341/141‑1 (MQ=255)
                     aGTACCAGATATAAAAATGAGCGATTTAAATTTTTCCAATACTTGTTTCGTTAATACATCTTTTACAGCGTAATCTTTCACATATAAACGTATTGTTAATTTTTGAGATAAATTTTTAATCGAAATGAAACTAGTATGTcc                                                                                                                          >  4:424611/1‑141 (MQ=255)
                        aCCAGATATAAAAATGAGCGATTTAAATTTTTCCAATACTTGTTTCGTTAATACATCTTTTACAGCGTAATCTTTCACATATAAACGTATTGTTGATTTTTGAGATAAATTTTTAATCGAAATGAAACTAGTATGTCCAGc                                                                                                                       <  1:564279/141‑1 (MQ=255)
                            gATATAAAAATGAGCGATTTAAATTTTTCCAACACTCATTTCGTTAATACAACTTTTACACCATAATCTTTCAAAAATAAAAGTATTTTTTATTCTTTAGATAAATTTTTAATCTAAATGAGACAAGCGTGTCGAGCtttt                                                                                                                   >  4:297941/1‑141 (MQ=255)
                             atatAAAAATGAGCGATTTAAATTTTTCCAATACTTGTTTCGTTAATACATCTTTTACAGCGTAATCTTTCACATATAAACGTATTGTTGATTTTTGAGATAAATTTTTAATCGAAATGAAATTAGTATGTCCAGCTTTTa                                                                                                                  <  3:191483/141‑1 (MQ=255)
                                taAAAATGAGTGATTTAAATTTTTCCAATACTTGTTTCGTTAATACATCTTTTACAGCGTAATCTTTCACATATAAACGTATTGTTGATTTTTGAGATAAATTTTTAATTGAAATGa                                                                                                                                       >  2:842672/1‑117 (MQ=255)
                                taAAAATGAGTGATTTAAATTTTTCCAATACTTGTTTCGTTAATACATCTTTTACAGCGTAATCTTTCACATATAAACGTATTGTTGATTTTTGAGATAAATTTTTAATTGAAATGa                                                                                                                                       <  1:842672/117‑1 (MQ=255)
                                taAAAATGAGCGATTTAAATTTTTCCAATACTTGTTTCGTTAATACATCTTTTACAGCGTAATCTTTCACATATAAACGTATTGTTGATTTTTGAGATAAATTTTTAATCGAAATGAAACTAGTATGTCCAGCTTTTAAAc                                                                                                               >  2:197236/1‑141 (MQ=255)
                                 aaaaaTGAGCGATTTAAATTTTTCCAATACTTGTTTCGTTAATACATCTTTTACAGCGTAATCTTTCACATATAAACGTATTGTTGATTTTTGAGATAAATTTTTAATCGAAATGAAACTAGTATGTCCAGCTTTTAAACt                                                                                                              >  1:679906/1‑141 (MQ=255)
                                     aTGAGCGATTTAAATTTTTCCAATACTTGTTTCGTTAATACATCTTTTACAGCGTAATCTTTCACATATAAACGTATTGTTGATTTTTGAGATAAATTTTTAATCGAAATGAAACTAGTATGTCCAGCTTTTAAACTTTGt                                                                                                          <  4:171309/141‑1 (MQ=255)
                                     aTGAGCGATTTAAATTTTTCCAATACTTGTTTCGTTAATACATCTTTTACAGCGTAATCTTTCACATATAAACGTATTGTTGATTTTTGAGATAAATTTTTAATCGAAATGAAACTAGTATGTCCAGCTTTTAAACTTTGt                                                                                                          <  2:985924/141‑1 (MQ=255)
                                     aTGAGCGATTTAAATTTTTCCAATACTTGTTTCGTTAATACATCTTTTACAGCGTAATCTTTCACATATAAACGTATTGTTGATTTTTGAGATAAATTTTTAATCGAAATGAAACTAGTATGTCAAGCTTTTAAACTTTGt                                                                                                          >  4:121393/1‑141 (MQ=255)
                                      tGAGCGATTTAAATTTTTCCAATACTTGTTTCGTTAATACATCTTTTACAGCGTAATCTTTCACATATAAACGTATTGTTGATTTTTGAGATAAATTTTTAATCGAAATGAAACTAGTATGTCCAGCTTTTAAACTTTGtt                                                                                                         >  1:558953/1‑141 (MQ=255)
                                       gAGCGATTTAAATTTTTCCAATACTTGTTTCGTTAATACATCTTTTACAGCGTAATCTTTCACATATAAACGTATTGTTGATTTTTGAGATAAATTTTTAATCGAAATGAAACTAGTATGTCCAGCTTTTAAACTTTGTTc                                                                                                        <  2:350273/141‑1 (MQ=255)
                                          cGATTTAAATTTTTCCAATACTTGTTTCGTTAATACATCTTTTACAGCGTAATCTTTCACATATAAACGTATTGTTGATTTTTGAGATAAATTTTTAATCGAAATGAAACTAGTATGTCCAGCTTTTAAACTTTGTTCGat                                                                                                     <  2:318803/141‑1 (MQ=255)
                                            aTTTAAATTTTTCCAATACTTGTTTCGTTAATACATCTTTTACAGCGTAATCTTTCACATATAAACGTATTGTTGATTTTTGAGATAAATTTTTAATCGAAATGAAACTAGTATGTTCAGCTTTTAGACTTTGTTCGatat                                                                                                   >  4:374524/1‑141 (MQ=255)
                                               tAAATTTTTCCAATACTTGTTTCGTTAATACATCTTTTACAGCGTAATCTTTCACATATAAACGTATTGTTGATTTTTGAGATAAATTTTTAATCGAAATGAAACTAGTATGTCCAGCTTTTAAACTTTGTTCGATAtttt                                                                                                <  2:191279/141‑1 (MQ=255)
                                                  aTTTTTCCAATACTTGTTTCGTCAATACATCTTTTACAGCGTAATCTTTCACATATAAACGTATTGTTGATTTTTGAGATAAATTTTTAATCGAAATGAAACTAGTATGTCCAGCTTTTAAACTTTGTTCGATATTTTTaa                                                                                             <  1:585684/141‑1 (MQ=255)
                                                   tttttCCAATACTTGTTTCGTTAATACATCTTTTACAGCGTAATCTTTCACATATAAACGTATTGTTGATTTTTGAGATAAATTTTTAATCGAAATGAAACTAGTATGTCCAGCTTTTAAACTTTGTTCGATATTTTTaaa                                                                                            <  1:493400/141‑1 (MQ=255)
                                                    ttttCCAATACTTGTTTCGTTAATACATCTTTTACAGCGTAATCTTTCACATATAAACGTATTGTTGATTTTTGAGATAAATTTTTAATCGAAATGAAACTAGTATGTCCAGCTTTTAAACTTTGTTCGATATTTTTAAAt                                                                                           <  1:331646/141‑1 (MQ=255)
                                                    ttttCCAATACTTGTTTCGTTAATACATCTTTTACAGCGTAATCTTTCACATATAAACGTATTGTTGATTTTTGAGATAAATTTTTAATCGAAATGAAACTAGTATGTCCAGCTTTTAAACTTTGTTCGATATTTTTAAAt                                                                                           <  4:245445/141‑1 (MQ=255)
                                                    ttttCCAATACTTGTTTCGTTAATACATCTTTTACAGCGTAATCTTTCACATATAAACGTATTGTTGATTTTTGAGATAAATTTTTAATCGAAATGAAACTAGTATGTCCAGCTTTTAAACTTTGTTCGATATTTTTAAAt                                                                                           <  3:58569/141‑1 (MQ=255)
                                                     tttCCAATACTTGTTTCGTTAATACATCTTTTACAGCGTAATCTTTCACATATAAACGTATTGTTGATTTTTGAGATAAATTTTTAATCGAAATGAAACTAGTATGTCCAGCTTTTAAACTTTGTTCGATATTTTTAAAtt                                                                                          <  3:63573/141‑1 (MQ=255)
                                                     tttCCAATACTTGTTTCGTTAATACATCTTTTACAGCGTAATCTTTCACATATAAACGTATTGTTGATTTTTGAGATAAATTTTTAATCGAAATGAAACTAGTATGTCCAGCTTTTAAACTTTGTTCGATATTTTTAAAtt                                                                                          <  4:236118/141‑1 (MQ=255)
                                                     tttCCAATACTTGTTTCGTTAATACATCTTTTACAGCGTAATCTTTCACATATAAACGTATTGTTGATTTTTGAGATAAATTTTTAATCGAAATGAAACTAGTATGTCCAGCTTTTAAACTTTGTTCGATATTTTTAAAtt                                                                                          <  2:50698/141‑1 (MQ=255)
                                                     tttCCAATACTTGTTTCGTTAATACATCTTTTACAGCGTAATCTTTCACATATAAACGTATTGTTGATTTTTGAGATAAATTTTTAATCGAAATGAAACTAGTATGTCCAGCTTTTAAACTTTGTTCGATATTTTTAAAtt                                                                                          <  1:446222/141‑1 (MQ=255)
                                                      ttCCAATACTTGTTTCGTTAATACATCTTTTACAGCGTAATCTTTCACATATAAACGTATTGTTGATTTTTGAGATAAATTTTTAATCGAAATGAAACTAGTATGTCCAGCTTTTAAACTTTGTTCGATATTTTTAAAttt                                                                                         <  2:1055092/141‑1 (MQ=255)
                                                         cAATACTTGTTTCGTTAATACATCTTTTACAGCGTAATCTTTCACATATAAACGTATTGTTGATTTTTGAGATAAATTTTTAATCGAAATGAAACTAGTATGTCCAGCTTTTAAACTTTGTTCGATATTTTTAAATTTATc                                                                                      >  1:269198/1‑141 (MQ=255)
                                                          aaTACTTGTTTCGTTAATACATCTTTTACAGCGTAATCTTTCACATATAAACGTATTGTTGATTTTTGAGATAAATTTTTAATCGAAATGAAACTAGTATGTCCAGCTTTTAAACTTTGTTCGATATTTTTAAATTTATCt                                                                                     >  2:175792/1‑141 (MQ=255)
                                                          aaTACTTGTTTCGTTAATACATCTTTTACAGCGTAATCTTTCACATATAAACGTATTGTTGATTTTTGAGATAAATTTTTAATCGAAATGAAACTAGTATGTCCAGCTTTTAAACTTTGTTCGATATTTTTAAATTTATCt                                                                                     <  2:1063466/141‑1 (MQ=255)
                                                          aaTACATGTTTCGTTAATACATCTTTTACAGCGTAATCTTTCACATATAAACGTATTGTTGATTTTTGAGATAAATTTTTAATCGAAATGAAACTAGTATGTCCAGCTTTTAAACTTTGTTCGATATTTTTAAATTTATCt                                                                                     <  2:479155/141‑1 (MQ=255)
                                                            tACTTGTTTCGTTAATACATCTTTTACAGCGTAATCTTTCACATATAAACGTATTGTTGATTTTTGAGATAAATTTTTAATCGAAATGAAACTAGTATGTCCAGCTTTTAAACTTTGTTCGATATTTTTAAATTTATCttt                                                                                   <  1:378471/141‑1 (MQ=255)
                                                                 gTTTCGTTAATACATCTTTTACAGCGTAATGGCGCGCATATAGACGTATTGTTGATTTTTGAGATAAATTTTTAATCGAAATGAAACTAGTATGTCCAGCTTTTAAACTTTGTTCGATATTTTTAAATTTATCTTTTAAAt                                                                              >  2:864632/1‑141 (MQ=255)
                                                                 gTTTCGTTAATACATCTTTTACAGCGTAATCTTTCACATATAAACGTATTGTTGATTTTTGAGATAAATTTTTAATCGAAATGAAACTAGTATGTCCAGCTTTTAAACTTTGTTCGATATTTTTAAATTTATCTTTTAAAt                                                                              >  3:225191/1‑141 (MQ=255)
                                                                     cGTTAATACATCTTTTACAGCGTAATCTTTCACATATAAACGTATTGTTGATTTTTGAGATAAATTTTTAATCGAAATGAAACTAGTATGTCCAGCTTTTAAACTTTGTTCGATATTTTTAAATTTATCTTTTAAATacaa                                                                          <  2:400287/141‑1 (MQ=255)
                                                                     cGTTAATACATCTTTTACAGCGTAATCTTTCACATATAAACGTATTGTTGATTTTTGAGATAAATTTTTAATCGAAATGAAACTAGTATGTCCAGCTTTTAAACTTTGTTCGATATTTTTAAATTTATCTTTTAAATacaa                                                                          >  3:241642/1‑141 (MQ=255)
                                                                       ttAATACATCTTTTACAGCGTAATCTTTCACATATAAACGTATTGTTGATTTTTGAGATAAATTTTTAATCGAAATGAAACTAGTATGTCCAGCTTTTAAACTTTGTTCGATATTTTTAAATTTATCTTTTAAATacaaca                                                                        <  3:39267/141‑1 (MQ=255)
                                                                                 ttttACAGCGTAATCTTTCACATATAAACGTATTGTTGATTTTTGAGATAAATTTTTAATCGAAATGAAACTAGTATGTCCAGCTTTTAAACTTTGTTCGATATTTTTAAATTTATCTTTTAAATACAACAACTGTTTCCg                                                              <  2:772982/141‑1 (MQ=255)
                                                                                 ttttACAGCGTAATCTTTCACATATAAACGTATTGTTGATTTTTGAGATAAATTTTTAATCGAAATGAAACTAGTATGTCCAGCTTTTAAACTTTGTTCGATATTTTTAAATTTATCTTTTAAATACAACAACTGTTTCCg                                                              >  2:681399/1‑141 (MQ=255)
                                                                                  tttACAGCGTAATCTTTCACATATAAACGTATTGTTGATTTTTGAGATAAATTTTTAATCGAAATGAAACTAGTATGTCCAGCTTTTAAACTTTGTTCGATATTTTTAAATTTATCTTTTAAATACAACAACTGTTTCCGt                                                             <  1:414600/141‑1 (MQ=255)
                                                                                    tACAGCGTAATCTTTCACATATAAACTTTTTGTTGATTTTTGAGATAAATTTTTAATCGAAATGAAACTAGTATGTCCAGCTTTTAAACTTTGTTCGATATTTTTAAATTTATCTTTTAAATACAACAACTGTTTCCGTaa                                                           <  1:267167/141‑1 (MQ=255)
                                                                                        gCGTAATCTTTCACATATAAACGTATTGTTGATTTTTGAGATAAATTTTTAATCGAAATGAAACTAGTATGTCCAGCTTTTAAACTTTGTTCGATATTTTTAAATTTATCTTTTAAATACAACAACTGTTTCCGTAATGAt                                                       <  2:576511/141‑1 (MQ=255)
                                                                                            aaTCTTTCACATATAAACGTATTGTTGATTTTTGAGATAAATTTTTAATCGAAATGAAACTAGTATGTCCAGCTTTTAAACTTTGTTCGATATTTTTAAATTTATCTTTTAAATACAACAACTGTTTCCGTAATGATTTaa                                                   <  1:1054862/141‑1 (MQ=255)
                                                                                             aTCTTTCACATATAAACGTATTGTTGATTTTTGAGATAAATTTTTAATCGAAATGAAACTAGTATGTCCAGCTTTTAAACTTTGTTCGATATTTTTAAATTTATCTTTTAAATACAACAACTGTTTCCGTAATGATTTAAc                                                  <  1:596488/141‑1 (MQ=255)
                                                                                             aTCTTTCACATATAAACGTATTGTTGATTTTTGAGATAAATTTTTAATCGAAATGAAACTAGTATGTCCAGCTTTTAAACTTTGTTCGATATTTTTAAATTTATCTTTTAAATACAACAACTGTTTCCGTAATGATTTAAc                                                  <  1:1086297/141‑1 (MQ=255)
                                                                                              tCTTTCACATATAAACGTATTGTTGATTTTTGAGATAAATTTTTAATCGAAATGAAACTAGTATGTCCAGCTTTTAAACTTTGTTCGATATTTTTAAATTTATCTTTTAAATACAACAACTGTTTCCGTAATGATTTAACt                                                 <  2:58283/141‑1 (MQ=255)
                                                                                              tCTTTCACATATAAACGTATTGGTGATTTTTGAGATAAATTTTTAATCGAAATGAAACTAGTATGTCCAGCTTTTAAACTTTGTTCGATATTTTTAAATTTATCTTTTAAATACAACAACTGTTGCCGTAATGATTTAACt                                                 <  1:973872/141‑1 (MQ=255)
                                                                                                tttCACATATAAACGTATTGTTGATTTTTGAGATAAATTTTTAATCGAAATGAAACTAGTATGTCCAGCTTTTAAACTTTGTTCGATATTTTTAAATTTATCTTTTAAATACAACAACTGTTTCCGTAATGATTTAACTGt                                               >  1:244782/1‑141 (MQ=255)
                                                                                                   cacaTATAAACGTATTGTTGATTTTTGAGATAAATTTTTAATCGAAATGAAACTAGTATGTCCAGCTTTTAAACTTTGTTCGATATTTTTAAATTTATCTTTTAAATACAACAACTGTTTCCGTAATGATTTAACTGtttt                                            >  2:621790/1‑141 (MQ=255)
                                                                                                   cacaTATAAACGTATTGTTGATTTTTGAGATAAATTTTTAATCGAAATGAAACTAGTATGTCCAGCTTTTAAACTTTGTTCGATATTTTTAAATTTATCTTTTAAATACAACAACTGTTTCCGTAATGATTTAACTGtttt                                            >  1:328507/1‑141 (MQ=255)
                                                                                                      atatAAACGTATTGTTGATTTTTGAGATAAATTTTTAATCGAAATGAAACTAGTATGTCCAGCTTTTAAACTTTGTTCGATATTTTTAAATTTATCTTTTAAATACAACAACTGTTTCCGTAATGATTTAACTGTTTTATg                                         <  2:641908/141‑1 (MQ=255)
                                                                                                        ataAACGTATTGTTGATTTTTGAGATAAATTTTTAATCGAAATGAAACTAGTATGTCCAGCTTTTAAACTTTGTTCGATATTTTTAAATTTATCTTTTAAATACAACAACTGTTTCCGTAATGATTTAACTGTTTTATGAc                                       <  2:830005/141‑1 (MQ=255)
                                                                                                         taAACGTATTGTTGATTTTTGAGATAAATTTTTAATCGAAATGAAACTAGTATGTCCAGCTTTTAAACTTTGTTCGATATTTTTAAATTTATCTTTTAAATACAACAACTGTTTCCGTAATGATTTAACTGTTTTATGACt                                      >  3:78026/1‑141 (MQ=255)
                                                                                                         taAACGTATTGTTGATTTTTGAGATAAATTTTTAATCGAAATGAAACTAGTATGTCCAGCTTTTAAACTTTGTTCGATATTTTTAAATTTATCTTTTAAATACAACAACTGTTTCCGTAATGATTTAACTGTTTTATGACt                                      >  2:790571/1‑141 (MQ=255)
                                                                                                         taAACGTATTGTTGATTTTTGAGATAAATTTTTAATCGAAATGAAACTAGTATGTCCAGCTTTTAAACTTTGTTCGATATTTTTAAATTTATCTTTTAAATACAACAACTGTTTCCGTAATGATTTAACTGTTTTATGACt                                      <  1:463608/141‑1 (MQ=255)
                                                                                                           aaCGTATTGTTGATTTTTGAGATAAATTTTTAATCGAAATGAAACTAGTATGTCCAGCTTTTAAACTTTGTTCGATATTTTTAAATTTATCTTTTAAATACAACAACTGTTTCCGTAATGATTTAACTGTTTTATGACTaa                                    <  3:121212/141‑1 (MQ=255)
                                                                                                               tATTGTTGATTTTTGAGATAAATTTTTAATCGAAATGAAACTAGTATGTCCAGCTTTTAAACTTTGTTCGATATTTTTAAATTTATCTTTTAAATACAACAACTGTTTCCGTAATGATTTAACTGTTTTATGACTAATGcc                                >  3:385290/1‑141 (MQ=255)
                                                                                                                aTTGTTGATTTTTGAGATAAATTTTTAATCGAAATGAAACTAGTATGTCCAGCTTTTAAACTTTGTTCGATATTTTTAAATTTATCTTTTAAATACAACAACTGTTTCCGTAATGATTTAACTGTTTTATGACTAATGCCa                               >  1:790860/1‑141 (MQ=255)
                                                                                                                 ttgttgATTTTTGAGATAAATTTTTAATCGAAATGAAACTAGTATGTCCAGCTTTTAAACTTTGTTCGATATTTTTAAATTTATCTTTTAAATACAACAACTGTTTCCGTAATGATTTAACTGTTTTATGACTAATGCCAt                              <  4:58628/141‑1 (MQ=255)
                                                                                                                  ggttgATGTTTGAGATAAATTTTTAATCGAAATGAAACTAGTATGTCCAGCTTTTAAACGTTGTTCGATATTTTTAAATTTATCTTTTAAATACAACAACTGTTTCCGTAATGATTTAACTGTTTTATGACTAATGCCAtt                             <  2:405852/140‑1 (MQ=255)
                                                                                                                   gttgATTTTTGAGATAAATTTTTAATCGAAATGAAACTAGTATGTCCAGCTTTTAAACTTTGTTCGATATTTTTAAATTTATCTTTTAAATACAACAACTGTTTCCGTAATGATTTAACTGTTTTATGACTACTGCCAttt                            >  4:431051/1‑140 (MQ=255)
                                                                                                                           ttGAGATAAATTTTTAATCGAAATGAAACTAGTATGTCCAGCTTTTAAACTTTGTTCGATATTTTTAAATTTATCTTTTAAATACAACAACTGTTTCCGTAATGATTTAACTGTTTTATGACTAATGCCATTGAATATTTc                    <  2:11618/141‑1 (MQ=255)
                                                                                                                             gagaTAAATTTTTAATCGAAATGAAACTAGTATGTCCAGCTTTTAAACTTTGTTCGATATTTTTAAATTTATCTTTTAAATACAACAACTGTTTCCGTAATGATTTAACTGTTTTATGACTAATGCCATTGAATATTTCTa                  <  2:358916/141‑1 (MQ=255)
                                                                                                                             gagaTAAATTTTTAATCGAAATGAAACTAGTATGTCCAGCTTTTAAACTTTGTTCGATATCTTTAAATTTATCTTTTAAATACAACAACTGTTTCCGTAATGATTTAACTGTTTTATGACTAATGCCATTGAATATTTCTa                  >  1:1024835/1‑141 (MQ=255)
                                                                                                                               gaTAAATTTTTAATTGAAATGAAACTAGTATGTCCAGCTTTTAAACTTTGTTCGATATTTTTAAATTTATCTTTTAAATACAACAACTGTTTCCGTAATGATTTAACTGTTTTATGACTAATGCCATTGAATATTTCTAGc                <  2:719813/141‑1 (MQ=255)
                                                                                                                               gaTAAATTTTTAATCGAAATGAAACTAGTATGTCCAGCTTTTAAACTTTGTTCGATATTTTTAAATTTATCTTTTAAATACAACAACTGTTTCCGTAATGATTTAACTGTTTTATGACTAATGCCATTGAATATTTCTAGc                >  1:610262/1‑141 (MQ=255)
                                                                                                                                 tAAATTTTTAATCGAAATGAAACTAGTATGTCCAGCTTTTAAACTTTGTTCGATATTTTTAAATTTATCTTTTAAATACAACAACTGTTTCCGTAATGATTTAACTTTTTTATGACTAATGCCATTGAATATTTCTAGCGt              <  2:914547/141‑1 (MQ=255)
                                                                                                                                 tAAATTTTTAATCGAAATGAAACTAGTATGTCCAGCTTTTAAACTTTGTTCGATATTTTTAAATTTATCTTTTAAATACAACAACTGTTTCCGTAATGATTTAACTGTTTTATGACTAATGCCATTGAATATTTCTAGCGt              <  2:382344/141‑1 (MQ=255)
                                                                                                                                 tAAATTTTTAATCGAAATGAAACTAGTATGTCCAGCTTTTAAACTTTGTTCGATATTTTTAAATTTATCTTTTAAATACAACAACTGTTTCCGTAATGATTTAACTGTTTTATGACTAATGCCATTGAATATTTCTAGCGt              <  2:417912/141‑1 (MQ=255)
                                                                                                                                 tAAATTTTTAATCGAAATGAAACTAGTATGTCCAGCTTTTAAACTTTGTTCGATATTTTTAAATTTATCTTTTAAATACAACAACTGTTTCCGTAATGATTTAACTGTTTTATGACTAATGCCATTGAATATTTCTAGCGt              <  1:186381/141‑1 (MQ=255)
                                                                                                                                 tAAATTTTTAATCGAAATGAAACTAGTATGTCCAGCTTTTAAACTTTGTTCGATATTTTTAAATTTATCTTTTAAATACAACAACTGTTTCCGTAATGATTTAACTGTTTTATGACTAATGCCATTGAATATTTCTAGCGt              <  1:1043641/141‑1 (MQ=255)
                                                                                                                                  aaaTTTTTAATCGAAATGAAACTAGTATGTCCAGCTTTTAAACTTTGTTCGATATTTTTAAATTTATCTTTTAAATACAACAACTGTTTCCGTAATGATTTAACTGTTTTATGACTAATGCCATTGAATATTTCTAGCGtt             <  1:16084/141‑1 (MQ=255)
                                                                                                                                   aaTTTTTAATCGAAATGAAACTAGTATGTCCAGCTTTTAAACTTTGTTCGATATTTTTAAATTTATCTTTTAAATACAACAACTGTTTCCGTAATGATTTAACTGTTTTATGACTAATGCCATTGAATATTTCTAGCGttt            >  4:414460/1‑141 (MQ=255)
                                                                                                                                       tttAATCGAAATGAAACTAGTATGTCCAGCTTTTAAACTTTGTTCGATATTTTTAAATTTATCTTTTAAATACAACAACTGTTTCCTTAATGATTTAACTGTTTTATGACTAATGCCATTGAATATTTCTAGCGTtttatt        <  1:496583/141‑1 (MQ=255)
                                                                                                                                       tttAATCGAAATGAAACTAGTATGTCCAGCTTTTAAACTTTGTTCGATATTTTTAAATTTATCTTTTAAATACAACAACTGTTTCCGTAATGATTTAACTGTTTTATGACTAATGCCATTGAATATTTCTAGCGTtttatt        >  3:250156/1‑141 (MQ=255)
                                                                                                                                         tAATCGAAATGAAACTAGTATGTCCAGCTTTTAAACTTTGTTCGATATTTTTAAATTTATCTTTTAAATACAACAACTGTTTCCGTAATGATTTAACTGTTTTATGACTAATGCCATTGAATATTTCTAGCGTtttattta      <  4:44935/141‑1 (MQ=255)
                                                                                                                                          aaTCGAAATGAAACTAGTATGTCCAGCTTTTAAACTTTGTTCGATATTTTTAAATTTATCTTTTAAATACAACAACTGTTTCCGTAATGATTTAACTGTTTTATGACTAATGCCATTGAATATTTCTAGCGTTTTATTTaa     >  1:197073/1‑141 (MQ=255)
                                                                                                                                           aTCGAAATGAAACTAGTATGTCCAGCTTTTAAACTTTGTTCGATATTTTTAAATTTATCTTTTAAATACAACAACTGTTTCCGTAATGATTTAACTGTTTTATGACTAATGCCATTGAATATTTCTAGCGTTTTATTTAAc    >  3:76703/1‑141 (MQ=255)
                                                                                                                                           aTCGAAATGAAACTAGTATGTCCAGCTTTTAAACTTTGTTCGATATTTTTAAATTTATCTTTTAAATACAACAACTGTTTCCGTAATGATTTAACTGTTTTATGACTAATGCCATTGAATATTTCTAGCGTTTTATTTAAc    >  1:795015/1‑141 (MQ=255)
                                                                                                                                             cGAAATGAAACTAGTATGTCCAGCTTTTAAACTTTGTTCGATATTTTTAAATTTATCTTTTAAATACAACAACTGTTTCCGTAATGATTTAACTGTTTTATGACTAATGCCATTGAATATTTCTAGCGTTTTATTTAACtt  >  2:220366/1‑141 (MQ=255)
                                                                                                                                             cGAAATGAAACTAGTATGTCCAGCTTTTAAACTTTGTTCGATATTTTTAAATTTATCTTTTAAATACAACAACTGTTTCCGTAATGATTTAACTGTTTTATGACTAATGCCATTGAATATTTCTAGCGTTTTATTTAACtt  <  2:688234/141‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                            
AAACGAATGATTAAATTTTAAAGTACCAGATATAAAAATGAGCGATTTAAATTTTTCCAATACTTGTTTCGTTAATACATCTTTTACAGCGTAATCTTTCACATATAAACGTATTGTTGATTTTTGAGATAAATTTTTAATCGAAATGAAACTAGTATGTCCAGCTTTTAAACTTTGTTCGATATTTTTAAATTTATCTTTTAAATACAACAACTGTTTCCGTAATGATTTAACTGTTTTATGACTAATGCCATTGAATATTTCTAGCGTTTTATTTAACTT  >  USA300TCH1516_ALE/1525963‑1526244

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: