BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	1,637,258	C→T	5.5%	A432A (GCG→GCA)	recN ←	DNA repair protein RecN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,637,258	0	C	T	5.5%	331.6 / 4.6	107	A432A (GCG→GCA)	recN	DNA repair protein RecN
Reads supporting (aligned to +/- strand): ref base C (50/51); new base T (4/2); total (54/53)
Fisher's exact test for biased strand distribution p-value = 6.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

Only 100 of 110 total aligned reads displayed.

GCATTTTCTCTGCCATTTTTTGTGCAGCTTGTCCAGATACACCTGAGTCAACCTCATCGAATAAAATTGCAGTTTGACCTCTCGATTTAACAAAAATACTTTTTAACGCTAACATAATTCTAGAAAGTTCTCCACCTGACGCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTGGACTGATTAAAAATTCTACAAATTCGATTCCATCAATATTCGGTTCTTCTAATTTTTTAAATGAAATCTCAAGATTTGCGTCTTTCATTTGTAAGTTTTG  >  USA300TCH1516_ALE/1637119‑1637398

gCATTTTCTCTGCCATTTNTTGTGCAGCTTGTCNAGANACACCTGAGTCAACCTCATCGAATAAAATTGCAGTTTGACCTCTCGATTTAACAAAAATACTTTTTAACGCTAACATAATTCTAGAAAGTTCTCCACCTGACg                                                                                                                                             >  3:352276/1‑141 (MQ=255)

 cATTTTCTCTGCCATTTTTTGTGCAGCTTGTCCAGATACACCTGAGTCAACCTCATCGAATAAAATTGCAGTTTGACCTCTCGATTTAACAAAAATACTTTTTAACGCTAACATAATTCTAGAAAGTTCTCCACCTGACGc                                                                                                                                            >  2:452457/1‑141 (MQ=255)

 cATTTTCTCTGCCATTTTTTGTGCAGCTTGTCCAGATACACCTGAGTCAACCTCATCGAATAAAATTGCAGTTTGACCTCTCGATTTAACAAAAATACTTTTTAACGCTAACATAATTCTAGAAAGTTCTCCACCTGACGc                                                                                                                                            <  1:440942/141‑1 (MQ=255)

     ttCTCTGCCATTTTTTGTGCAGCTTGTCCAGATACACCTGAGTCAACCTCATCGAATAAAATTGCAGTTTGACCTCTCGATTTAACAAAAATACTTTTTAACGCTAACATAATTCTAGAAAGTTCTCCACCTGACGCAAtt                                                                                                                                        <  2:588261/141‑1 (MQ=255)

     ttCTCTGCCATTTTTTGTGCAGCTTGTCCAGATACACCTGAGTCAACCTCATCGAATAAAATTGCAGTTTGACCTCTCGATTTAACAAAAATACTTTTTAACGCTAACATAATTCTAGAAAGTTCTCCACCTGACGCAAtt                                                                                                                                        <  2:588298/141‑1 (MQ=255)

     ttCTCTGCCATTTTTTGTGCAGCTTGTCCAGATACACCTGAGTCAACCTCATCGAATAAAATTGCAGTTTGACCTCTCGATTTAACAAAAATACTTTTTAACGCTAACATAATTCTAGAAAGTTCTCCACCTGACGCAAtt                                                                                                                                        <  3:145180/141‑1 (MQ=255)

     ttCTCTGCCATTTTTTGTGCAGCTTGTCCAGATACACCTGAGTCAACCTCATCGAATAAAATTGCAGTTTGACCTCTCGATTTAACAAAAATACTTTTTAACGCTAACATAATTCTAGAAAGTTCTCCACCTGACGCAAtt                                                                                                                                        >  1:874204/1‑141 (MQ=255)

       ctctGCCATTTTTTTTTTTTTTTTTCCAGATACACCTGAGTCAACCTCATCGAATAAAATTGCAGTTTGACCTCTCGATTTAACAAAAATACTTTTTAACGCTAACATAATTCTAGAAAGTTCTCCACCTGACGCAAtttt                                                                                                                                      >  2:9973/1‑141 (MQ=255)

          tGCCATTTTTTGTGCAGCTTGTCCAGATACACCTGAGTCAACCTCATCGAATAAAATTGCAGTTTGACCTCTCGATTTAACAAAAATACTTTTTAACGCTAACATAATTCTAGAAAGTTCTCCACCTGACGCAATtttatt                                                                                                                                   <  1:674385/141‑1 (MQ=255)

           gCCATTTTTTGTGCAGCTTGTCCAGATACACCTGAGTCAACCTCATCGAATAAAATTGCCGTTTGACCTCTCGATTTAACAAAAATACTTTTTAACGCTAACATAATTCTAGAAAGTTCTCCACCTGACGCAATtttattt                                                                                                                                  >  1:692364/1‑141 (MQ=255)

           gCCATTTTTTGTGCAGCTTGTCCAGATACACCTGAGTCAACCTCATCGAATAAAATTGCAGTTTGACCTCTCGATTTAACAAAAATACTTTTTAACGCTAACATAATTCTAGAAAGTTCTCCACCTGACGCAATtttattt                                                                                                                                  <  1:730149/141‑1 (MQ=255)

               ttttttGTGCAGCTTGTCCAGATACACCTGAGTCAACCTCATCGAATAAAATTGCAGTTTGACCTCTCAAAATAAAAAAAATACTTTTTAACGCTAACATAATTCTAGAAAGTTCTCCACCTGACGCAATTTTATTTAAAc                                                                                                                              <  2:711454/141‑1 (MQ=255)

                   ttGTGCAGCTTGTCCAGATACACCTGAGTCAACCTCATCGAATAAAATTGCAGTTTGACCTCTCGATTTAACAAAAATACTTTTTAACGCTAACATAATTCTAGAAAGTTCTCCACCTGACGCAATTTTATTTAAACtttt                                                                                                                          <  1:1080363/141‑1 (MQ=255)

                    tgtgCAGCTTGTCCAGATACACCTGAGTCAACCTCATCGAATAAAATTGCAGTTTGACCTCTCGATTTAACAAAAATACTTTTTAACGCTAACATAATTCTAGAAAGTTCTCCACCTGACGCAATTTTATTTAAACttttt                                                                                                                         >  4:107317/1‑141 (MQ=255)

                     gtgCAGCTTGTCCAGATACACCTGAGTCAACCTCATCGAATAAAATTGCAGTTTGACCTCTCGATTTAACAAAAATACTTTTTAACGCTAACATAATTCTAGAAAGTTCTCCACCTGACGCAATTTTATTTAAACTTTTTa                                                                                                                        <  1:1012195/141‑1 (MQ=255)

                     gtgCAGCTTGTCCAGATACACCTGAGTCAACCTCATCGAATAAAATTGCAGTTTGACCTCTCGATTTAACAAAAATACTTTTTAACGATAACATAATTCTAGAAAGTTCTCCACCTGACGCAATTTTATTTAAACTTTTTa                                                                                                                        >  2:193960/1‑141 (MQ=255)

                      tgCAGCTTGTCCAGATACACCTGAGTCAACCTCATCGAATAAAATTGCAGTTTGACCTCTCGATTTAACAAAAATACTTTTTAACGCTAACATAATTCTAGAAAGTTCTCCACCTGACGCAATTTTATTTAAACTTTTTaa                                                                                                                       >  3:203752/1‑141 (MQ=255)

                       gCAGCTTGTCCAGATACACCTGAGTCAACCTCATCGAATAAAATTGCAGTTTGACCTCTCGATTTAACAAAAATACTTTTTAACGCTAACATAATTCTAGAAAGTTCTCCACCTGACGCAATTTTATTTAAACTTTTTAAt                                                                                                                      >  1:659078/1‑141 (MQ=255)

                             tGTCCAGATACACCTGAGTCAACCTCATCGAATAAAATTGCAGTTTGACCTCTCGATTTAACAAAAATACTTTTTAACGCTAACATAATTCTAGAAAGTTCTCCACCTGACGCAATTTTATTTAAACTTTTTAATGGTTcc                                                                                                                <  4:309250/141‑1 (MQ=255)

                               tCCAGATACACCTGAGTCAACCTCATCGAATAAAATTGCAGTTTGACCTCTCGATTTAACAAAAATACTTTTTAACGCTAACATAATTCTAGAAAGTTCTCCACCTGACGCAATTTTATTTAAACTTTTTAATGGTTcccc                                                                                                              >  1:629127/1‑141 (MQ=255)

                                caaagtACACCTGAGTCAACCTCATCGAATAAAATTGCAGTTTGACCTCTCGATTTAACAAAAATACTTTTTAACGCTAACATAATTCTAGAAAGTTCTCCACCTGACGCAATTTTATTTAAACTTTTTAATGGTTCCCCt                                                                                                             >  2:277465/6‑141 (MQ=255)

                                  aGATACACCTGAGTCAACCTCATCGAATAAAATTGCAGTTTGACCTCTCGATTTAACAAAAATACTTTTTAACGCTAACATAATTCTAGAAAGTTCTCCACCTGACGCAATTTTATTTAAACTTTTTAATGGTTCCCCttt                                                                                                           <  1:162828/141‑1 (MQ=255)

                                     tACACCTGAGTCAACCTCATCGAATAAAATTGCAGTTTGACCTCTCGATTTAACAAAAATACTTTTTAACGCTAACATAATTCTAGAAAGTTCTCCACCTGACGCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTAtt                                                                                                        >  1:116540/1‑141 (MQ=255)

                                       cacCTGAGTCAACCTCATCGAATAAAATTGCAGTTTGACCTCTCGATTTAACCAAAATACTTTTTAACGCTAACATAATTCTAGAAAGTTCTCCACCTGACGCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTg                                                                                                      >  2:180772/1‑141 (MQ=255)

                                         ccTGAGTCAACCTCATCGAATAAAATTGCAGTTTGACCTCTCGATTTAACAAAAATACTTTTTAACGCTAACATAATTCTAGAAAGTTCTCCACCTGACGCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTGGa                                                                                                    <  1:305056/141‑1 (MQ=255)

                                         ccTGAGTCAACCTCATCGAATAAAATTGCAGTTTGACCTCTCGATTTAACAAAAATACTTTTTAACGCTAACATAATTCTAGAAAGTTCTCCACCTGACGCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTGGa                                                                                                    <  1:1043524/141‑1 (MQ=255)

                                         ccTGAGTCAACCTCATCGAATAAAATTGCAGTTTGACCTCTCGATTTAACAAAAATACTTTTTAACGCTAACATAATTCTAGAAAGTTCTCCACCTGACGCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTGGa                                                                                                    >  4:114305/1‑141 (MQ=255)

                                           tGAGTCAACCTCATCGAATAAAATTGCAGTTTGACCTCTCGATTTAACAAAAATACTTTTTAACGCTAACATAATTCTAGAAAGTTCTCCACCTGACGCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTGGACt                                                                                                  >  3:455787/1‑141 (MQ=255)

                                              gTCAACCTCATCGAATAAAATTGCAGTTTGACCTCTCGATTTAACAAAAATACTTTTTAACGCTAACATAATTCTAGAAAGTTCTCCACCTGACGCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTGGACTGAt                                                                                               <  2:1069881/141‑1 (MQ=255)

                                                cAACCTCATCGAATAAAATTGCAGTTTGACCTCTCGATTTAACAAAAATACTTTTTAACGCTAACATAATTCTAGAAAGTTCTCCACCTGACGCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTGGACTGATTa                                                                                             <  1:146540/141‑1 (MQ=255)

                                                 aaCCTCATCGAATAAAATTGCAGTTTGACCTCTCGATTTAACAAAAATACTTTTTAACGCTAACATAATTCTAGAAAGTTCTCCACCTGACGCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTGGACTGATTaa                                                                                            >  1:942094/1‑141 (MQ=255)

                                                 aaCCTCATCGAATAAAATTGCAGTTTGACCTCTCGATTTAACAAAAATACTTTTTAACGCTAACATAATTCTAGAAAGTTCTCCACCTGACGCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTGGACTGATTaa                                                                                            >  2:130109/1‑141 (MQ=255)

                                                 aaCCTCATCGAATAAAATTGCAGTTTGACCTCTCGATTTAACAAAAATACTTTTTAACGCTAACATAATTCTAGAAAGTTCTCCACCTGACGCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTGGACTGATTaa                                                                                            >  1:422805/1‑141 (MQ=255)

                                                  acatCATCGAATAAAATTGCAGTTGGACCTCTCGATTTAACAAAAATACTTTTTAACGCTAACATAATTCTAGAAAGTTCTCCACCTGACGCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTGGACTGATTaaa                                                                                           >  4:315012/4‑141 (MQ=255)

                                                   ccTCATCGAATAAAATTGCAGTTTGACCTCTCGATTTAACAAAAATACTTTTTAACGCTAACATAATTCTAGAAAGTTCTCCACCTGACGCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTGGACTGATTaaaa                                                                                          >  2:257179/1‑141 (MQ=255)

                                                       aTCGAATAAAATTGCAGTTTGACCTCTCGATTTAACAAAAATACTTTTTAACGCTAACATAATTCTAGAAAGTTCTCCACCTGACGCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTGGACTGATTAAAAATTc                                                                                      >  3:337381/1‑141 (MQ=255)

                                                       aTCAAATAAAATTTCAGTTTTCCCTCTCTATTTAACAAATATACTTTTTAACGCTAACATAATTCTAGAAAGTTCTCCACCTTACTCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTGGACTGATTAAAAATTc                                                                                      <  2:741276/141‑1 (MQ=255)

                                                         cGAATAAAATTGCAGTTTGACCTCTCGATTTAACAAAAATACTTTTTAACGCTAACATAATTCTAGAAAGTTCTCCACCTGACGCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTGGACTGATTAAAAATTCTa                                                                                    <  2:715293/141‑1 (MQ=255)

                                                         cGAATAAAATTGCAGTTTGACCTCTCGATTTAACAAAAATACTTTTTAACGCTAACATAATTCTAGAAAGTTCTCCACCTGACGCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTGGACTGATTAAAAATTCTa                                                                                    <  3:148165/141‑1 (MQ=255)

                                                          gAATAAAATTGCATTTTGACCTCTCGATTTAACAAAAATACTTTTTAACGCTAACATAATTCTAGAAAGTTCTCCACCTGACGCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTGGACTGATTAAAAATTCTAc                                                                                   <  2:482318/141‑1 (MQ=255)

                                                          gAATAAAATTGCAGTTTGACCTCTCGATTTAACAAAAATACTTTTTAACGCTAACATAATTCTAGAAAGTTCTCCACCTGACGCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTGGACTGATTAAAAATTCTAc                                                                                   <  1:637699/141‑1 (MQ=255)

                                                          gAATAAAATTGCAGTTTGACCTCTCGATTTAACAAAAATACTTTTTAACGCTAACATAATTCTAGAAAGTTCTCCACCTGACGCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTGGACTGATTAAAAATTCTAc                                                                                   >  1:377864/1‑141 (MQ=255)

                                                          gAATAAAATTGCAGTTTGACCTCTCGATTTAACAAAAATACTTTTTAACGCTAACATAATTCTAGAAAGTTCTCCACCTGACGCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTGGACTGATTAAAAATTCTAc                                                                                   >  2:167932/1‑141 (MQ=255)

                                                            aTAAAATTGCAGTTTGACCTCTCGATTTAACAAAAATACTTTTTAACGCTAACATAATTCTAGAAAGTTCTCCACCTGACGCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTGGACTGATTAAAAATTCTACag                                                                                 <  1:1030621/141‑2 (MQ=255)

                                                            aTAAAATTGCAGTTTGACCTCTCGATTTAACAAAAATACTTTTTAACGCTAACATAATTCTAGAAAGTTCTCCACCTGACGCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTGGACTGATTAAAAATTCTACaa                                                                                 <  2:175257/141‑1 (MQ=255)

                                                             tAAAATTGCAGTTTGACCTCTCGATTTAACAAAAATACTTTTTAACGCTAACATAATTCTAGAAAGTTCTCCACCTGATGCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTGGACTGATTAAAAAGTCTACaaa                                                                                >  4:364753/1‑141 (MQ=255)

                                                              aaaaTTGCAGTTTGACCTCTCGATTTAACAAAAATACTTTTTAACGCTAACATAATTCTAGAAAGTTCTCCACCTGACGCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTGGACTGATTAAAAATTCTACAAAt                                                                               >  1:657041/1‑141 (MQ=255)

                                                              aaaaTTGCAGTTTGACCTCTCGATTTAACAAAAATACTTTTTAACGCTAACATAATTCTAGAAAGTTCTCCACCTGACGCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTGGACTGATTAAAAATTCTACAAAt                                                                               >  3:206639/1‑141 (MQ=255)

                                                               aaaTTGCAGTTTGACCTCTCGATTTAACAAAAATACTTTTTAACGCAAACATAATTCTAGAAAGTTCTCCACCTGATGCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTGGACTGATTAAAAATTCTACAAAtt                                                                              >  3:377048/1‑141 (MQ=255)

                                                                 aTTGCAGTTTGACCTCTCGATTTAACAAAAATACTTTTTAACGCTAACATAATTCTAGAAAGTTCTCCACCTGACGCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTGGACTGATTAAAAATTCTACAAATTCg                                                                            >  1:403233/1‑141 (MQ=255)

                                                                    gCAGTTTGACCTCTCGATTTAACAAAAATACTTTTTAACGCTAACATAATTCTAGAAAGTTCTCCACCTGATGCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTGGACTGATTAAAAATTCTACAAATTCGAtt                                                                         >  1:352477/1‑141 (MQ=255)

                                                                    gCAGTTTGACCTCTCGATTTAACAAAAATACTTTTTAACGCTAACATAATTCTAGAAAGTTCTCCACCTGACGCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTGGACTGATTAAAAATTCTACAAATTCGAtt                                                                         >  1:270253/1‑141 (MQ=255)

                                                                    gCAGTTTGACCTCTCGATTTAACAAAAATACTTTTTAACGCTAACATAATTCTAGAAAGTTATCCACCTGACGCAATTTTATTTAAACTTTTTAATTGTTCCCCTTTATTTGGACTGATTAAAAATTCTACAAATTCGAtt                                                                         >  4:225073/1‑141 (MQ=255)

                                                                         ttGACCTCTCGATTTAACAAAAATACTTTTTAACGCTAACATAATTCTAGAAAGTTCTCCACCTGACGCAATTTTATTTAAACTTTTTAATGGTTCCCCTATATTTGGACTGATTAAAAATTCTACAAATTCGATTCCATc                                                                    >  4:402491/1‑141 (MQ=255)

                                                                              ctctCGATTTAACAAAAATACTTTTTAACGCTAACATAATTCTAGAAAGTTCTCCACCTGACGCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTGGACTGATTAAAAATTCTACAAATTCGATTCCATCAatat                                                               <  2:379646/141‑1 (MQ=255)

                                                                               tctcGATTTAACAAAAATACTTTTTAACGCTAACATAATTCTAGAAAGTTCTCCACCTGACGCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTGGACTGATTAAAAATTCTACAAATTCGATTCCATCAATAtt                                                              <  4:323273/141‑1 (MQ=255)

                                                                               tctcGATTTAACAAAAATACTTTTTAACGCTAACATAATTCTAGAAAGTTCTCCACCTGACGAAATTTTATTTAAACTTTTTAATGGTTCCACTTTATTTGGACTGATTATAAATTCTAGAAATTCAATTCCATCACTAtt                                                              >  4:208312/1‑141 (MQ=255)

                                                                                      ttAACATAAATACTTTTTAACGCTGACCTAATTCTAGATAGTTCCCCTCCTTCTGCAATTTTATTTACCGTTTTCAATGGTTCCCCTTTATTTGGACTGATTATAAATTCTAAAATTGCGATCCCGTTAATATTCGGttct                                                       <  4:20763/141‑1 (MQ=255)

                                                                                        aaCAAAAATACTTTTTAACGCTAACATAATTCTAGAAAGTTCTCCACCTGACGCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTGGACTGATTAAAAATTCTACAAATTCGATTCCATCAATATTCGGttcttc                                                     >  2:999878/1‑141 (MQ=255)

                                                                                        aaCAAAAATACTTTTTAACGCTAACATAATTCTAGAAAGTTCTCCACCTGACGCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTGGACTGATTAAAAATTCTACAAATTCGATTCCATCAATATTCGGttcttc                                                     <  2:659078/141‑1 (MQ=255)

                                                                                         aCAAAAATACTTTTTAACGCTAACATAATTCTAGAAAGTTCTCCACCTGACGCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTGGACTGATTAAAAATTCTACAAATTCGATTCCATCAATATTCGGTtcttct                                                    >  1:268529/1‑141 (MQ=255)

                                                                                         aCAAAAATACTTTTTAACGCTAACATAATTCTAGAAAGTTCTCCACCTGACGCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTGGACTGATTAAAAATTCTACAAATTCGATTCCATCAATATTCGGTtcttct                                                    <  2:551906/141‑1 (MQ=255)

                                                                                          cAAAAATACTTTTTAACGCTAACATAATTCTAGAAAGTTCTCCACCTGACGCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTGGACTGATTAAAAATTCTACAAATTCGATTCCATCAATATTCGGTTCTTCTa                                                   <  2:749824/141‑1 (MQ=255)

                                                                                           aaaaaTACTTTTTAACGCTAACATAATTCTAGAAAGTTCTCCACCTGACGCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTGGACTGATTAAAAATTCTACAAATTCGATTCCATCAATATTCGGTTCTTCTaa                                                  <  1:799850/141‑1 (MQ=255)

                                                                                            aaaaTACTTTTTAACGCTAACATAATTCTAGAAAGTTCTCCACCTGATTCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTGGACTGATTAAATATTCTACAAATTCGATTCCATCAATATTCGGTTCTTCTAAt                                                 <  2:128217/141‑1 (MQ=255)

                                                                                             aaaTACTTTTTAACGCTAACATAATTCTAGAAAGTTCTCCACCTGACGCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTGGACTGATTAAAAATTCTACAAAt                                                                               <  1:123928/110‑1 (MQ=255)

                                                                                             aaaTACTTTTTAACGCTAACATAATTCTAGAAAGTTCTCCACCTGACGCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTGGACTGATTAAAAATTCTACAAAt                                                                               >  2:123928/1‑110 (MQ=255)

                                                                                              aaTACTTTTTAACGCTAACATAATTCTAGAAAGTTCTCCACCTGACGCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTGGACTGATTAAAAATTCTACAAATTCGATTCCATCAATATTCGGTTCTTCTAAttt                                               >  1:522096/1‑141 (MQ=255)

                                                                                               aTACTTTTTAACGCTAACATAATTCTAGAAAGTTCTCCACCTGATGCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTGGACTGATTAAAAATTCTACAAATTCGATTCCATCAATATTCGGTTCTTCTAAtttt                                              >  2:346577/1‑141 (MQ=255)

                                                                                                tACTTTTTAACGCTAACATAATTCTAGAAAGTTCTCCACCTGACGCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTGGACTGATTAAAAATTCTACAAATTCGATTCCATCAATATTCGGTTCTTCTAAttttt                                             >  1:38143/1‑141 (MQ=255)

                                                                                                 aCTTTTTAACGCTAACATAATTCTAGAAAGTTCTCCACCTGACGCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTGGACTGATTAAAAATTCTACAAATTCGATTCCATCAATATTCGGTTCTTCTAAtttttt                                            <  2:776627/141‑1 (MQ=255)

                                                                                                  cTTTTTAACGCTAACATAATTCTAGAAAGTTCTCCACCTGACGCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTGGACTGATTAAAAATTCTACAAATTCGATTCCATCAATATTCGGTTCTTCTAATTTTTTa                                           >  2:444110/1‑141 (MQ=255)

                                                                                                      ttAACGCTAACATAATTCTAGAAAGTTCTCCACCTGACGCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTGGACTGATTAAAAATTCTACAAATTCGATTCCATCAATATTCGGTTCTTCTAATTTTTTAAATg                                       >  1:1048641/1‑141 (MQ=255)

                                                                                                      ttAACGCTAACATAATTCTAGAAAGTTCTCCACCTGACGCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTGGACTGATTAAAAATTCTACAAATTCGATTCCATCAATATTCGGTTCTTCTAATTTTTTAAATg                                       <  3:139365/141‑1 (MQ=255)

                                                                                                       tAACGCTAACATAATTCTAGAAAGTTCTCCACCTGACGCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTGGACTGATTAAAAATTCTACAAATTCGATTCCATCAATATTCGGTTCTTCTAATTTTTTAAATGa                                      <  1:265023/141‑1 (MQ=255)

                                                                                                           gCTAACATAATTCTAGAAAGTTCTCCACCTGACGCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTGGACTGATTAAAAATTCTACAAATTCGATTCCATCAATATTCGGTTCTTCTAATTTTTTAAATGAAAtc                                  <  1:141773/141‑1 (MQ=255)

                                                                                                           gCTAACATAATTCTAGAAAGTTCTCCACCTGACGCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTGGACTGATTAAAAATTCTACAAATTCGATTCCATCAATATTCGGTTCTTCTAATTTTTTAAATGAAAtc                                  >  3:398414/1‑141 (MQ=255)

                                                                                                           gCTAACATAATTCTAGAAAGTTCTCCACCTGACGCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTGGACTGATTAAAAATTCTACAAATTCGATTCCATCAATATTCGGTTCTTCTAATTTTTTAAATGAAAtc                                  >  3:222665/1‑141 (MQ=255)

                                                                                                            cTAACATAATTCTAGAAAGTTCTCCACCTGACGCACTTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTGGACTGATTAAAAATTCTACAAATTCGATTCCATCAATATTCGGTTCTTCTAATTTTTTAAATGAActct                                 >  1:594204/1‑141 (MQ=255)

                                                                                                             tAACATAATTCTAGAAAGTTCTCCACCTGACGCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTGGACTGATTAAAAATTCTACAAATTCGATTCCATCAATATTCGGTTCTTCTAATTTTTTAAATGAAAtctc                                <  3:198207/141‑1 (MQ=255)

                                                                                                             tAACATAATTCTAGAAAGTTCTCCACCTGACGCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTGGACTGATTAAAAATTCTACAAATTCGATTCCATCAATATTCGGTTCTTCTAATTTTTTAAATGAAAtctc                                <  2:1032563/141‑1 (MQ=255)

                                                                                                             tAACATAATTCTAGAAAGTTCTCCACCTGACGCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTGGACTGATTAAAAATTCTACAAATTCGATTCCATCAATATTCGGTTCTTCTAATTTTTTAAATGAAAtctc                                <  2:942094/141‑1 (MQ=255)

                                                                                                             tAACATAATTCTAGAAAGTTCTCCACCTGACGCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTGGACTGATTAAAAATTCTACAAATTCGATTCCATCAATATTCGGTTCTTCTAATTTTTTAAATGAAAtctc                                <  3:122189/141‑1 (MQ=255)

                                                                                                             tAACATAAGTCTAGAAAGTTCTCCACCTGACGCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTGGACTGATTAAAAATTCTACAAATTCGATTCCATCAATATTCGGTTCTTCTAATTTTTTAAATGAAAtctc                                <  1:8118/141‑1 (MQ=255)

                                                                                                                cATAATTCTAGAAAGTTCTCCACCTGACGCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTGGACTGATTAAAAATTCTACAAATTCGATTCCATCAATATTCGGTTCTTCTAATTTTTTAAATGAAATCTCAAg                             >  2:994046/1‑141 (MQ=255)

                                                                                                                cATAATTCTAGAAAGTTCTCCACCTGACGCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTGGACTGATTAAAAATTCTACAAATTCGATTCCATCAATATTCGGTTCTTCTAATTTTTTAAATGAAATCTCAAg                             >  1:69773/1‑141 (MQ=255)

                                                                                                                      tCTAGAAAGTTCTCCACCTGACGCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTGGACTGATTAAAAATTCTACAAATTCGATTCCATCAATATTCGGTTCTTCTAATTTTTTAAATGAAATCTCAAGATTTGc                       <  3:368432/141‑1 (MQ=255)

                                                                                                                        tAGAAAGTTCTCCACCTGACGCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTGGACTGATTAAAAATTCTACAAATTCGATTCCATCAATATTCGGTTCTTCTAATTTTTTAAATGAAATCTCAAGATTTGCGt                     <  1:484698/141‑1 (MQ=255)

                                                                                                                        tAGAAAGTTCTCCACCTGACGCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTGGACTGATTAAAAATTCTACAAATTCGATTCCATCAATATTCGGTTCTTCTAATTTTTTAAATGAAATCTCAAGATTTGCGt                     <  1:766741/141‑1 (MQ=255)

                                                                                                                            aaGTTCTCCACCTGACGCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTGGACTGATTAAAAATTCTACAAATTCGATTCCATCAATATTCGGTTCTTCTAATTTTTTAAATGAAATCTCAAGATTTGCGTCttt                 >  1:426348/1‑141 (MQ=255)

                                                                                                                            aaGTTCTCCACATGACGCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTGGACTGATTAAAAATTCTACAAATTCGATTCCATCAATATTCGGTTCTTCTAATTTTTTAAATGAAATCTCAAGATTTGCGTCttt                 <  2:475080/141‑1 (MQ=255)

                                                                                                                             aGTTCTCCACCTGACGCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTGGACTGATTAAAAATTCTACAAATTCGATTCCATCAATATTCGGTTCTTCTAATTTTTTAAATGAAATCTCAAGATTTGCGTCTTTc                >  2:941733/1‑141 (MQ=255)

                                                                                                                              gTTCTCCACCTGACGCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTGGACTGATTAAAAATTCTACAAATTCGATTCCATCAATATTCGGTTCTTCTAATTTTTTAAATGAAATCTCAAGATTTGCGTCTTTCa               <  2:578626/141‑1 (MQ=255)

                                                                                                                                tctcCACCTGACGCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTGGACTGATTAAAAATTCTACAAATTCGATTCCATCAATATTCGGTTCTTCTAATTTTTTAAATGAAATCTCAAGATTTGCGTCTTTCAtt             <  2:412691/141‑1 (MQ=255)

                                                                                                                                 ctcCACCTGACGCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTGGACTGATTAAAAATTCTACAAATTCGATTCCATCAATATTCGGTTCTTCTAATTTTTTAAATGAAATCTCAAGATTTGCGTCTTTCAttt            >  1:706269/1‑141 (MQ=255)

                                                                                                                                   ccACCTGACGCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTGGACTGATTAAAAATTCTACAAATTCGATTCCATCAATATTCGGTTCTTCTAATTTTTTAAATGAAATCTCACGATTTGCGTCTTTCATTTGt          >  1:930232/1‑141 (MQ=255)

                                                                                                                                   ccACCTGACGCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTGGACTGATTAAAAATTCTACAAATTCGATTCCATCAATATTCGGTTCTTCTAATTTTTTAAATGAAATCTCAAGATTTGCGTCTTTCATTTGt          <  1:890825/141‑1 (MQ=255)

                                                                                                                                           cGCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTGGACTGATTAAAAATTCTACAAATTCGATTCCATCAATATTCGGTTCTTCTAATTTTTTAAATGAAATCTCAAGATTTGCGTCTTTCATTTGTAAGTTTTg  <  2:69533/141‑1 (MQ=255)

                                                                                                                                           cGCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTGGACTGATTAAAAATTCTACAAATTCGATTCCATCAATATTCGGTTCTTCTAATTTTTTAAATGAAATCTCAAGATTTGCGTCTTTCATTTGTAAGTTTTg  <  2:997979/141‑1 (MQ=255)

                                                                                                                                           cGCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTGGACTGATTAAAAATTCTACAAATTCGATTCCATCAATATTCGGTTCTTCTAATTTTTTAAATGAAATCTCAAGATTTGCGTCTTTCATTTGTAAGTTTTg  <  2:377864/141‑1 (MQ=255)

GCATTTTCTCTGCCATTTTTTGTGCAGCTTGTCCAGATACACCTGAGTCAACCTCATCGAATAAAATTGCAGTTTGACCTCTCGATTTAACAAAAATACTTTTTAACGCTAACATAATTCTAGAAAGTTCTCCACCTGACGCAATTTTATTTAAACTTTTTAATGGTTCCCCTTTATTTGGACTGATTAAAAATTCTACAAATTCGATTCCATCAATATTCGGTTCTTCTAATTTTTTAAATGAAATCTCAAGATTTGCGTCTTTCATTTGTAAGTTTTG  >  USA300TCH1516_ALE/1637119‑1637398

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`