Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 1,825,570 A→T 5.5% D70E (GAT→GAA tpx ← putative thiol peroxidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,825,5700AT 5.5% 392.3 / 9.7 126D70E (GAT→GAAtpxputative thiol peroxidase
Reads supporting (aligned to +/- strand):  ref base A (54/65);  new base T (5/2);  total (59/67)
Fisher's exact test for biased strand distribution p-value = 2.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.88e-01

Only 100 of 129 total aligned reads displayed.

CCATAGTTTTCACCAAATGATAAGTCACGGTGGTCACTTAATGTAATGACATTGTCTAAACCTGCTGAAGCGCACCATCTTTTTTGTGCGAATGGTAAGTCTGCTGAAATTGTAAGCACAATCCCCTCTTCTTTAGAAGCATCAGAGTTGAATTTGCGAGTCTGCTGATCACAAACACCTGTATCAATTGATGGTACCACACTAATTAATTTCTTTTTACCAGCATAATCTGCTAATGTTACTTGATTTAAGTCATTATCTAACACTGTAAAATCAGGTGC  >  USA300TCH1516_ALE/1825430‑1825710
                                                                                                                                            |                                                                                                                                            
ccATAGTTTTCACCAAATGATAAGTCACGGTGGTCACTTAATGTAATGACATTGTCTAAACCTGCTGAAGCGCACCATCTTTTTTGTGCGAATGGTAAGTCTGCTGAAATTGTAAGCACAATCCCCTCTTCTTTAGAAGCa                                                                                                                                              >  2:632171/1‑141 (MQ=255)
 cATAGTTTTCACAAAATGATAAGTCACGGTGGTCACTTAATGTAATGACATTGTCTAAACCTGCTGAAGCGCACCATCTTTTTTGTGCGAATGGTAAGTCTGCTGAAATTGTAAGCACATTCCCCTCTTCTTTAGAAGCAt                                                                                                                                             <  4:107693/141‑1 (MQ=255)
     gTTTTCACCAAATGATAAGTCACGGTGGTCACTTAATGTAATGACATTGTCTAAACCTGCTGAAGCGCACCATCTTTTTTGTGCGAATGGTAAGTCTGCTGAAATTGTAAGCACAATCCCCTCTTCTTTAGAAGCATCaga                                                                                                                                         >  4:371981/1‑141 (MQ=255)
         tCACCAAATGATAAGTCACGGTGGTCACTTAATGTAATGACATTGTCTAAACCTGCCGACGCGAACCATCTTTTTTGTGCGAATGGTAAGTCTGCTGAAATTGTAAGCACAATCCCCTCTTCTTTAGAAGCATCAGAGTTg                                                                                                                                     <  1:214015/141‑1 (MQ=255)
          cACCAAATGATAAGTCACGGTGGTCACTTAATGTAATGACATTGTCTAAACCTGCTGAAGCGCACCATCTTTTTTGTGCGAATGGTAAGTCTGCTGAAATTGTAAGCACAATCCCCTCTTCTTTAGAAGCATCAGAGTTGa                                                                                                                                    >  3:219358/1‑141 (MQ=255)
           aCCAAATGATAAGTCACGGTGGTCACTTAATGTAATGACATTGTCTAAACCTGCTGAAGCGCACCATCTTTTTTGTGCGAATGGTAAGTCTGCTGAAATTGTAAGCACAATCCCCTCTTCTTTAGAAGCATCAGAGTTGaa                                                                                                                                   <  3:289197/141‑1 (MQ=255)
            ccAAATGATAAGTCACGGTGGTCACTTAATGTAATGACATTGTCTAAACCTGCTGAAGCGCACCATCTTTTTTGTGCGAATGGTAAGTCTGCTGAAATTGTAAGCACAATCCCCTCTTCTTTAGAAGCATCAGAGTTGAAt                                                                                                                                  <  1:639702/141‑1 (MQ=255)
            ccAAATGATAAGTCACGGTGGTCACTTAATGTAATGACATTGTCTAAACCTGCTGAAGCGCACCATCTTTTTTGTGCGAATGGTAAGTATGCTGAAATTGTAAGCGCAATCCCCTCTTCTTTAGAAGCATGAGAGTTGAAt                                                                                                                                  >  1:584936/1‑141 (MQ=255)
             cAAATGATAAGTCACGGTGGTCACTTAATGTAATGACATTGTCTAAACCTGCTGAAGCGCACCATCTTTTTTGTGCGAATGGTAAGTCTGCTGAAATTGTAAGCACAATCCCCTCTTCTTTAGAAGCATCAGAGTTGAAtt                                                                                                                                 >  1:549857/1‑141 (MQ=255)
              aaaTGATAAGTCACGGTGGTCACTTAATGTAATGACATTGTCTAAACCTGCTGAAGCGCACCATCTTTTTTGTGCGAATGGTAAGTCTGCTGAAATTGTAAGCACAATCCCCTCTTCTTTAGAAGCATCAGAGTTGAAttt                                                                                                                                <  1:1075085/141‑1 (MQ=255)
               aaTGATAAGTCACGGTGGTCACTTAATGTAATGACATTGTCTAAACCTGCTGAAGCGCACCATCTTTTTTGTGCGAATGGTAAGTCTGCTGAAATTGTAAGCACAATCCCCTCTTCTTTAGAAGCATCAGAGTTGAATTTg                                                                                                                               <  4:282892/141‑1 (MQ=255)
               aaTGATAAGTCACGGTGGTCACTTAATGTAATGACATTGTCTAAACCTGCTGAAGCGCACCATCTTTTTTGTGCGAATGGTAAGTCTGCTGAAATTGTAAGCACAATCCCCTCTTCTTTAGAAGCATCAGAGTTGAATTTg                                                                                                                               <  1:318609/141‑1 (MQ=255)
                  gATAAGTCACGGTGGTCACTTAATGTAATGACATTGTCTAAACCTGCTGAAGCGCACCATCTTTTTTGTGCGAATGGTAAGTCTGCTGAAATTGTAAGCACAATCCCCTCTTCTTTAGAAGCTTCAGAGTTGAATTTGCGa                                                                                                                            <  1:844595/141‑1 (MQ=255)
                     aaGTCACGGTGGTCACTTAATGTAATGACATTGTCTAAACCTGCTGAAGCGCACCATCTTTTTTGTGCGAATGGTAAGTCTGCTGAAATTGTAAGCACAATCCCCTCTTCTTTAGAAGCATCAGAGTTGAATTTGCGAGTc                                                                                                                         <  4:213650/141‑1 (MQ=255)
                     aaGTCACGGTGGTCACTTAATGTAATGACATTGTCTAAACCTGCTGAAGCGCACCATCTTTTTTGTGCGAATGGTAAGTCTGCTGAAATTGTAAGCACAAGCCCCTCTTCTTTAGAAGCATCAGAGTTGAATTTGCGAGTc                                                                                                                         <  1:1046562/141‑1 (MQ=255)
                      aGTCACGGTGGTCACTTAATGTAATGACATTGTCTAAACCTGCTGAAGCGCACCATCTTTTTTGTGCGAATGGTAAGTCTGCTGAAATTGTAAGCACAATCCCCTCTTCTTTAGAAGCATCAGAGTTGAATTTGCGAGTct                                                                                                                        >  3:176036/1‑141 (MQ=255)
                         cACGGTGGTCACTTAATGTAATGACATTGTCTAAACCTGCTGAAGCGCACCATCTTTTTTGTGCGAATGGTAAGTCTGCTGAAATTGTAAGCACAATCCCCTCTTCTTTAGAAGCATCAGAGTTGAATTTGCGAGTctgct                                                                                                                     <  1:62905/141‑1 (MQ=255)
                            ggtggtCACTTAATGTAATGACATTGTCTAAACCTGCTGAAGCGCACCATCTTTTTTGTGCGAATGGTAAGTCTGCTGAAATTGTAAGCACAATCCCCTCTTCTTTAGAAGCATCAGAGTTGAATTTGCGAGTCTGCTGAt                                                                                                                  >  2:669590/1‑141 (MQ=255)
                              tggtCACTAAATGTAATGACATTGTCTAAACCTGCTGAAGCGCACCATCTTTTTTGTGCGAATGGTAAGTCTGCTGAAATTGTAAGCACAATCCCCTCTGCTTTAGAAGCATCAGAGTTGAATTTGCGAGTCTGCTGATca                                                                                                                <  4:304831/141‑1 (MQ=255)
                                 tCACTTAATGTAATGACATTGTCTAAACCTGCTGAAGCGCACCATCTTTTTTGTGCGAATGGTAAGTCTGCTGAAATTGTAAGCACAATCCCCTCTTCTTTAGAAGCTTCAGAGTTGAATTTGCGAGTCTGCTGATCACaa                                                                                                             >  2:995318/1‑141 (MQ=255)
                                   aCTTAATGTAATGACATTGTCTAAACCTGCTGAAGCGCACCATCTTTTTTGTGCGAATGGTAAGTCTGCTGAAATTGTAAGCACAATCCCCTCTTCTTTAGAAGCATCAGAGTTGAATTTGCGAGTCTGCTGATCACAAac                                                                                                           <  2:835185/141‑1 (MQ=255)
                                      taatgtaatgACATTGTCTAAACCTGCTTAAGAGCACCATCTTTTTTGTGCGAATGGTAAGTCTGCTGAAATTGTAAGCACAATCCCCTCTTCTTTAGAAGCATCAGAGTTGAATTTGCGAGTCTGCTGATCACAAACAcc                                                                                                        <  3:404249/141‑1 (MQ=255)
                                      taatgtaatgACATTGTCTAAACCTGCTGAAGCGCACCATCTTTTTTGTGCGAATGGTAAGTCTGCTGAAATTGTAAGCACAATCCCCTCTTCTTTAGAAGCATCAGAGTTGAATTTGCGAGTCTGCTGATCACAAACTcc                                                                                                        <  2:478071/141‑1 (MQ=255)
                                      taatgtaatgACATTGTCTAAACCTGCTGAAGCGCACCATCTTTTTTGTGCGAATGGTAAGTCTGCTGAAATTGTAAGCACAATCCCCTCTTCTTTAGAAGCATCAGAGTTGAATTTGCGAGTCTGCTGATCACAAACAcc                                                                                                        <  1:609180/141‑1 (MQ=255)
                                       aatgtaatgACCTTGTCTAAACCTGCTGAAGCGCACCATCTTATTTGTGCGAATGGTAAGTCTGCTGAAATTGTAAGCACAATCCCCTCTTCTTTAGAAGCATCAGAGTTGAATTTGCGAGTCTGCTGATCACAAACACCt                                                                                                       >  4:267997/1‑141 (MQ=255)
                                         tgtaatgACATTGTCTAAACCTGCTGAAGCGCACCATCTTTTTTGTGCGAATGGTAAGTCTGCTGAAATTGTAAGCACAATCCCCTCTTCTTTAGAAGCATCAGAGTTGAATTTGCGAGTCTGCTGATCACAAACACCTGt                                                                                                     <  1:687529/141‑1 (MQ=255)
                                          gtaatgACATTGTCTAAACCTGCTGAAGCGCACCATCTTTTTTGTGCGAATGGTAATTCTGCTGAAATTGTAAGCACAATCCCCTCTTCTTTAGAAGCATCAGAGTTGAATTTGCGAGTCTGCTGATCACAAACACCTGTa                                                                                                    <  2:60102/141‑1 (MQ=255)
                                          gtaatgACATTGTCTAAACCTGCTGAAGCGCACCATCTTTTTTGTGCGAATGGTAAGTCTGCTGAAATTGTAAGCACAATCCCCTCTTCTTTAGAAGCTTCAGAGTTGAATTTGCGAGTCTGCTGATCACAAACACCTGTa                                                                                                    >  2:40604/1‑141 (MQ=255)
                                          gtaatgACATTGTCTAAACCTGCTGAAGCGCACCATCTTTTTTGTGCGAATGGTAAGTCTGCTGAAATTGTAAGCACAATCCCCTCTTCTTTAGAAGCATCAGAGTTGAATTTGCGAGTCTGCTGATCACAAACACCTGTa                                                                                                    >  1:116870/1‑141 (MQ=255)
                                               gACATTGTCTAAACCTGCTGAAGCGCACCATCTTTTTTGTGCGAATGGTAAGTCTGCTGAAATTGTAAGCACAATCCCCTCTTCTTTAGAAGCATCAGAGTTGAATTTGCGAGTCTGCTGATCACAAACACCTGTATCAAt                                                                                               <  2:132099/141‑1 (MQ=255)
                                               gACATTGTCTAAACCTGATGAAGCGAACCTTCTTTTTTGTGCGCATGGTAAGTCTGCTGAAATTGTAAGCACAATCCCCTCTTCTTTAGAAGCATCACAGTAGAATTTGCGAGTCTGCTGATCACAAACACCTGTATCAAt                                                                                               <  2:158402/141‑1 (MQ=255)
                                                   ttGTCTAAACCTGCTGAAGCGCACCATCTTTTTTGTGCGAATGGTAAGTCTGCTGAAATTGTAAGCACAATCCCCTCTTCTTTAGAAGCTTCAGAGTTGAATTTGCGAGTCTGCTGATCACAAACACCTGTATCAATTGAt                                                                                           <  1:442499/141‑1 (MQ=255)
                                                    tGTCTACACCTGCTTAAGCGCACCATCTTTTTTGTGCGAATGGTAAGTCTGCTGAAATTGTAAGCACAATCCCCTCTTCTTTAGAAGCATCAGAGTTGAATTTGCGAGTCTGCTCATCACAAACACCTGTATCAATTGATg                                                                                          <  4:314041/141‑1 (MQ=255)
                                                       cTAAACCTGCTGAAGCGCACCATCTTTTTTGTGTGAATGGTAAGTCTGCTGAAATTGTAAGCACAATCCCCTCTTCTTTAGAAGCATCAGAGTTGAATTTGCGAGTCTGCTGATCACAAACACCTGTATCAATTGATGGTa                                                                                       >  2:791236/1‑141 (MQ=255)
                                                       cTAAACCTGCTGAAGCGCACCATCTTTTTTGTGCGAATGGTAAGTCTGCTGAAATTGTAAGCACAATCCCCTCTTCTTTAGAAGCATCAGAGTTGAATTTGCGAGTCTGCTGATCACAAACACCTGTATCAATTGATGGTa                                                                                       >  2:324465/1‑141 (MQ=255)
                                                            ccTGCTGAAGCGCACCATCTTTTTTGTGCGAATGGTAAGTCTGCTGAAATTGTAAGCACAATCCCCTCTTCTTTAGAAGCATCAGAGTTGAATTTGCGAGTCTGCTGATCACAAACACCTGTATCAATTGATGGTACcaca                                                                                  >  1:561223/1‑141 (MQ=255)
                                                              tgctgAAGCGCACCATCTTTTTTGTGCGAATGGTAAGTCTGCTGAAATTGTAAGCACAATCCCCTCTTCTTTAGAAGCATCAGAGTTGAATTTGCGAGTCTGCTGATCACAAACACCTGTATCAATTGATGGTACCACACt                                                                                <  2:847782/141‑1 (MQ=255)
                                                                 tgAAGCGCACCATCTTTTTTGTGCGAATGGTAAGTCTGCTGAAATTGTAAGCACAATCCCCTCTTCTTTAGAAGCATCAGAGTTGAATTTGCGAGTCTGCTGATCACAAACACCTGTATCAATTGATGGTACCACACtaat                                                                             >  4:211532/1‑141 (MQ=255)
                                                                 tgAAGCGCACCATCTTTTTTGTGCGAATGGTAAGTCTGCTGAAATTGTAAGCACAATCCCCTCTTCTTTAGAAGCATCAGAGTTGAATTTGCGAGTCTGCTGATCACAAACACCTGTATCAATTGATGGTACCACACtaat                                                                             <  1:651005/141‑1 (MQ=255)
                                                                  gAAGCGCACCATCTTTTTTTTTTTTTTTTTAAGTCTGATGAAATTGTAAGCACAATCTCCTCTTCTTTAGAAGCATCAGAGTTGAATTTGCGAGTCTGCTCATCACAAACACCTGTATCAATTGATGGTACCATACttatt                                                                            >  2:1010497/1‑141 (MQ=255)
                                                                   aaGCGCACCATCTTTTTTGTGCGAATGGTAAGTCTGCTGAAATTGTAAGCACAATCCCCTCTTCTTTAGAAGCATCAGAGTTGAATTTGCGAGTCTGCTGATCACAAACACCTGTATCAATTGATGGTACCACACtaatta                                                                           <  2:260584/141‑1 (MQ=255)
                                                                   aaGCGCACCATCTTTTTTGTGCGAATGGTAAGTCTGCTGAAATTGTAAGCACAATCCCCTCTTCTTTACAAGCATCAGAGTTGAATTTGCGAGTCTGCTGATCACAAACACCTGTATCAATTGATGGTACCACACtaatta                                                                           >  3:31965/1‑141 (MQ=255)
                                                                      cgcCCCATCTTTTTTGTGCGAATGGTAAGTCTGCTGAAATTGTAAGCACAATCCCCTCTTCTTTAGAAGCATCAGAGTTGAATTTGCGAGTCTGCTGATCACAAACACCTGTATCAATTGATGGTACCACACTaattaatt                                                                        <  1:500770/141‑1 (MQ=255)
                                                                       gcACCATCTTTTTTGTGCGAATGGTAAGTCTGCTGAAATTGTAAGCACAATCCCCTCTTCTTTAGAAGCATCAGAGTTGAATTTGCGAGTCTGCTGATCACAAACACCTGTATCAATTGATGGTACCACACTAATTAAttt                                                                       >  1:127866/1‑141 (MQ=255)
                                                                        cACCATCTTTTTTGTGCGAATGGTAAGTCTGCTGAAATTGTAAGCACAATCCCCTCTTCTTTAGAAGCATCAGAGTTGAATTTGCGAGTCTGCTGATCACAAACACCTGTATCAATTGATGGTACCACACTAATTAATTTc                                                                      <  1:651173/141‑1 (MQ=255)
                                                                         aCCATCTTTTTTGTGCGAATGGTAAGTCTGCTGAAATTGTAAGCACAATCCCCTCTTCTTTAGAAGCATCAGAGTTGAATTTGCGAGTCTGCTGATCACAAACACCTGTATCAATTGATGGTACCACACTAATTAATTTCt                                                                     >  1:385183/1‑141 (MQ=255)
                                                                              cTTTTTTGTGCGAATGGTAAGTCTGCTGAAATTGTAAGCACAATCCCCTCTTCTTTAGAAGCATCAGAGTTGAATTTGCGAGTCTGCTGATCACAAACACCTGTATCAATTGATGGTACCACACTAATTAATTTCTTTTTa                                                                <  2:243262/141‑1 (MQ=255)
                                                                                tttttGTGCGAATGGTAAGTCTGCTGAAATTGTAAGCACAATCCCCTCTTCTTTAGAAGCATCAGAGTTGAATTTGCGAGTCTGCTGATCACAAACACCTGTATCAATTGATGGTACCACACTAATTAATTTCTTTTTAcc                                                              <  1:303406/141‑1 (MQ=255)
                                                                                 ttttGTGCGAATGGTAAGTCTGCTGAAATTGTAAGCACAATCCCCTCTTCTTTAGAAGCATCAGAGTTGAATTTGCGAGTCTGCTGATCACAAACACCTGTATCAATTGATGGTACCACACTAATTAATTTCTTTTTACCa                                                             <  3:149157/141‑1 (MQ=255)
                                                                                     gtgtGAATAGTAAGTCTGCTGAAATTGTTAGCACAATCCCCTCTTCTGTAGAAGTATCAGAGTTGAATTTGCGAGTCTGCTGATCACAAACACCTGTATCAATTGATGGCACCACACTTATTAATTTCTTTTAACCAACAt                                                         <  4:46399/141‑1 (MQ=255)
                                                                                     gtgCGAATGGTAAGTCTGCTGAAATTGTAAGCACAATCCCCTCTTCTTTAGAAGCATCAGAGTTGAATTTGCGAGTCTGCTGATCACAAACACCTGTATCAATTGATGGTACCACACTAATTAATTTCTTTTTACCAGCAt                                                         >  1:690746/1‑141 (MQ=255)
                                                                                     gtgCGAATGGTAAGTCTACTGAAATTGTAAGCACAATCCCCACCTCTTCAAAAGCATCAGAGTTGAATTTGCGAGTCTGCTGATCACAAACACCTGTATCAATTGATGGTACCACACTAATTAACTTCTTTTTACCAGCAt                                                         <  2:309139/141‑1 (MQ=255)
                                                                                      tgCGAATGGTAAGTCTGCTGAAATTGTAAGCACAATCCCCTCTTCTTTAGAAGCATCAGAGTTGAATTTGCGAGTCTGCTGATCACAAACACCTGTATCAATTGATGGTACCACACTAATTAATTTCTTTTTACCAGCATa                                                        >  3:454391/1‑141 (MQ=255)
                                                                                       gCGAATGGTAAGTCTGCTGAAATTGTAAGCACAATCCCCTCTTCTTTAGAAGCATCAGAGTTGAATTTGCGAGTCTGCTGATCACAAACACCTGTATCAATTGATGGTACCACACTAATTAATTTCTTTTTACCAGCATaa                                                       >  1:83593/1‑141 (MQ=255)
                                                                                        cGAATGGTAAGTCTGCTGAAATTGTAAGCACAATCCCCTCTTCTTTAGAAGCATCAGAGTTGAATTTGCGAGTCTGCTGATCACAAACACCTGTATCAATTGATGGTACCACACTAATTAATTTCTTTTTACCAGCATAAt                                                      >  1:601332/1‑141 (MQ=255)
                                                                                          aaTGGTAAGTCTGCTGAAATTGTAAGCACAATCCCCTCTTCTTTAGAAGCATCAGAGTTGAATTTGCGAGTCTGCTGATCACAAACACCTGTATCAATTGATGGTACCACACTAATTAATTTCTTTTTACCAGCATAATCt                                                    <  3:290900/141‑1 (MQ=255)
                                                                                               tAAGTCTGCTGAAATTGTAAGCACAATCCCCTCTTCTTTAGAAGCATCAGAGTTGAATTTGCGAGTCTGCTGATCACACACACCTGTATCAATTGATGGTACCACACTAATTAATTTCTTTTTACCAGCATAATCTGCTaa                                               >  2:573597/1‑141 (MQ=255)
                                                                                               tAAGTCTGCTGAAATTGTAAGCACAATCCCCTCTTCTTTAGAAGCATCAGAGTTGAATTTGCGAGTCTGCTGATCACAAACACCTGTATCAATTGATGGTACCACACTAATTAATTTCTTTTTACCAGCATAATCTGCTaa                                               <  1:47525/141‑1 (MQ=255)
                                                                                                aaGTCTGCTGAAATTGTAAGCACAATCCCCTCTTCTTTAGAAGCATCAGAGTTGAATTTGCGAGTCTGCTGATCACAAACACCTGTATCAATTGATGGTACCACACTAATTAATTTCTTTTTACCAGCATAATCTGCTAAt                                              >  1:185418/1‑141 (MQ=255)
                                                                                                  gTCTGCTGAAATTGTAAGCACAATCCCCTCTTCTTTAGAAGCATCAGAGTTGAATTTGCGAGTCTGCTGATCACAAACACCTGTATCAATTGATGGTACCACACTAATTAATTTCTTTTTACCAGCATAATCTGCTAATGt                                            >  2:708294/1‑141 (MQ=255)
                                                                                                   tCTGCTGAAATTGTAAGCACAATCCCCTCTTCTTTAGAAGCATCAGAGTTGAATTTGCGAGTCTGCTGATCACAAACACCTGTATCAATTGATGGTACCACACTAATTAATTTCTTTTTACCAGCATAATCTGCTAATGtt                                           <  2:1062609/141‑1 (MQ=255)
                                                                                                     tgctgAAATTGTAAGCACAATCCCCTCTTCTTTAGAAGCATCAGAGTTGAATTTGCGAGTCTGCTGATCACAAACACCTGTATCAATTGATGGTACCACACTAATTAATTTCTTTTTACCAGCATAATCTGCTAATGTTAc                                         <  1:967424/141‑1 (MQ=255)
                                                                                                     tgatgAAAATGTAAACACAATCCCCTCTTCTTTAGAAACATCAGAGTTGAATTTGCGAGTCTGCTGATCACAAACACCTGTATCAATTGATAGTACCACACTAATTAATTTATTTTTACCAGCATAATCTGCTAATGTTAc                                         <  2:703182/138‑1 (MQ=255)
                                                                                                       ctgAAATTGTAAGCACAATCCCCTCTTCTTTAGAAGCATCAGAGTTGAATTTGCGAGTCTGCTGATCACAAACACCTGTATCAATTGATGGTACCACACTAATTAATTTCTTTTTACCAGCATAATCTGCTAATGTTACtt                                       <  2:452690/141‑1 (MQ=255)
                                                                                                        tgAAATTGTAAGCACAATCCCCTCTTCTTTAGAAGCATCAGAGTTGAATTTGCGAGTCTGCTGATCACAAACACCTGTATCAATTGATGGTACCACACTAATTAATTTCTTTTTACCAGCATAATCTGCTAATGTTACTTg                                      <  2:695553/141‑1 (MQ=255)
                                                                                                         gAAATTGTAAGCACAATCCCCTCTTCTTTAGAAGCATCAGAGTTGAATTTGCGAGTCTGCTGATCACAAACACCTGTATCAATTGATGGTACCACACTAATTAATTTCTTTTTACCAGCATAATCTGCTAATGTTACTTGa                                     <  1:853870/141‑1 (MQ=255)
                                                                                                          aaaTTGTAAGCACAATCCCCTCTTCTTTAGAAGCATCAGAGTTGAATTTGCGAGTCTGCTGATCACAAACACCTGTATCAATTGATGGTACCACACTAATTAATTTCTTTTTACCAGCATAATCTGCTAATGTTACTTGAt                                    <  3:59877/141‑1 (MQ=255)
                                                                                                           aaTTGTAAGCACAATCCCCTCTTCTTTAGAAGCATCAGAGTTGAATTTGCGAGTCTGCTGATCACAAACACCTGTATCAATTGATGGTACCACACTAATTAATTTCTTTTTACCAGCATAATCTGCTAATGTTACTTGAtt                                   >  3:116845/1‑141 (MQ=255)
                                                                                                           aaTTGTAAGCACAATCCCCTCTTCTTTAGAAGCATCAGAGTTGAATTTGCGAGTCTGCTGATCACAAACACCTGTATCAATTGATGGTACCACACTAATTAATTTCTTTTTACCAGCATAATCTGCTAATGTTACTTGAtt                                   >  1:664265/1‑141 (MQ=255)
                                                                                                            aTTGTAAGCACAATCCCCTCTTCTTTAGAAGCATCAGAGTTGAATTTGCGAGTCTGCTGATCACAAACACCTGTATCAATTGATGGTACCACACTAATTAATTTCTTTTTACCAGCATAATCTGCTAATGTTACTTGAttt                                  <  1:533622/141‑1 (MQ=255)
                                                                                                            aTTGTAAGCACAATCCCCTCTTCTTTAGAAGCATCAGAGTTGAATTTGCGAGTCTGCTGATCACAAACACCTGTATCAATTGATGGTACCACACTAATTAATTTCTTTTTACCAGCATAATCTGCTAATGTTACTTGAttt                                  >  2:557745/1‑141 (MQ=255)
                                                                                                              tGTAAGCACAATCCCCTCTTCTTTAGAAGCTTCAGAGTTGAATTTGCGAGTCTGCTGATCACAAACACCTGTATCAATTGATGGTACCACACTAATTAATTTCTTTTTACCAGCATAATCTGCTAATGTTACTTGATTTaa                                >  2:435915/1‑141 (MQ=255)
                                                                                                              tGTAAGCACAATCCCCTCTTCTTTAGAAGCATCAGAGTTGAATTTGCGAGTCTGCTGATCACAAACACCTGTATCAATTGATGGTACCACACTAATTAATTTCTTTTTACCAGCATAATCTGCTAATGTTACTTGATTTaa                                >  1:493286/1‑141 (MQ=255)
                                                                                                               gTAAGCACAATCCCCTCTTCTTTAGAAGCATCAGAGTTGAATTTGCGAGTCTGCTGATCACAAACACCTGTATCAATTGATGCTACCACACTAATTAATTTCTTTTTACCAGCATAATCTGCTAATGTTACTTGATTTAAg                               <  2:636187/141‑1 (MQ=255)
                                                                                                                 aaGCACAATCCCCTCTTCTTTAGAAGCATCAGCGTTGAATTTGCGAGTCTGCTGATCACAAACACCTGTATCAATTGATGGTACCACACTAATTAATTTCTTTTTACCAGCATAATCTGCTAATGTTACTTGATTTAAGTc                             >  2:476652/1‑141 (MQ=255)
                                                                                                                 aaGCACAATCCCCTCTTCTTTAGAAGCATCAGAGTTGAATTTGCGAGTCTGCTGATCACAAACACCTGTATCAATTGATGGTACCACACTAATTAATTTCTTTTTACCAGCATAATCTGCTAATGTTACTTGATTTAAGTc                             >  4:178458/1‑141 (MQ=255)
                                                                                                                 aaGCACAATCCCCTCTTCTTTAGAAGCATCAGAGTTGAATTTGCGAGTCTGCTGATCACAAACACCTGTATCAATTGATGGTACCACACTAATTAATTTCTTTTTACCAGCATAATCTGCTAATGTTACTTGATTTAAGTc                             <  2:868928/141‑1 (MQ=255)
                                                                                                                  aGCACAATCCCCTCTTCTTTAGAAGCTTCAGAGTTGAATTTGCGAGTCTGCTGATCACAAACACCTGTATCAATTGATGGTACCACACTAATTAATTTCTTTTTACCAGCATAATCTGCTAATGTTACTTGATTTAAGTCa                            >  2:450097/1‑141 (MQ=255)
                                                                                                                     acaATCCCCTCTTCTTTAGAAGCATCAGAGTTGAATTTGCGAGTCTGCTGATCACAAACACCTGTATCAATTGATGGTACCACACTAATTAATTTCTTTTTACCAGCATAATCTGCTAATGTTACTTGATTTAAGTCATTa                         >  1:974492/1‑141 (MQ=255)
                                                                                                                     acaATCCCCTCTTCTTTAGAAGCATCAGAGTTGAATTTGCGAGTCTGCTGATCACAAACACCTGTATCAATTGATGGTACCACACTAAGTAATTTCTTTTTACCAGCATAATCTGCTAATGTTACTTGATTTAAGTGATTa                         >  4:107252/1‑141 (MQ=255)
                                                                                                                      caATCCCCTCTTCTTTAGAAGCATCAGAGTTGAATTTGCGAGTCTGCTGATCACAAACACCTGTATCAATTGATGGTACCACACTAATTAATTTCTTTTTACCAGCATAATCTGCTAATGTTACTTGATTTAAGTCATTAt                        >  2:823454/1‑141 (MQ=255)
                                                                                                                      caATCCCCTCTTCTTTAGAAGCATCAGAGTTGAATTTGCGAGTCTGCTGATCACAAACACATGTATCAATTGATGGTACCACACTAATTAATTTCTTTTTACCAGCATAATCTGCTAATGTTACTTGATTTAAGTCATTAt                        >  3:238174/1‑141 (MQ=255)
                                                                                                                        aTCCCCTCTTCTTTAGAAGCATCAGAGTTGAATTTGCGAGTCTGCTGATCACAAACACCTGTATCAATTGATGGTACCACACTAATTAATTTCTTTCTACCAGCATAATCTGCTAATGTTACTTGATTTAAGTCATTATCt                      >  2:650035/1‑141 (MQ=255)
                                                                                                                          ccccTCTTCTTTAGAAGCATCAGAGTTGAATTTGCGAGTCTGCTGATCACAAACACCTGTATCAATTGATGGTACCACACTAATTAATTTCTTTTTACCAGCATAATCTGCTAATGTTACTTGATTTAAGTCATTATCTaa                    >  1:271565/1‑141 (MQ=255)
                                                                                                                           cccTCTTCTTTAGAAGCATCAGAGTTGAATTTGCGAGTCTGCTGATcaca                                                                                                              >  1:1016482/1‑50 (MQ=255)
                                                                                                                           cccTCTTCTTTAGAAGCATCAGAGTTGAATTTGCGAGTCTGCTGATcaca                                                                                                              <  2:1016482/50‑1 (MQ=255)
                                                                                                                           cccTCTTCTTTAGAAGCATCAGAGTTGAATTTGCGAGTCTGCTGATCACAAACACCTGTATCTATTGATGGTACCACACTAATTAATTTCTTTTTACCAGCATAATCTGCTAATGTTACTTGATTTAAGTCATTATCTAac                   >  2:206324/1‑141 (MQ=255)
                                                                                                                             ctctTCTTTAGAAGCATCAGAGTTGAATTTGCGAGTCTGCTGATCACAAACACCTGTATCAATTGATGGTACCACACTAATTAATTTCTTTTTACCAGCATAATCTGCTAATGTTACTTGATTTAAGTCATTATCTAacac                 <  1:539243/141‑1 (MQ=255)
                                                                                                                               cttcttTAGAAGCATCAGAGTTGAATTTGCGAGTCTGCTGATCACAAACACCTGTATCAATTGATGGTACCACACTAATTAATTTCTTTTTACCCGCATAATCTGCTAATGTTACTTGATTTAAGTCATTATCTAACACTg               <  3:377923/141‑1 (MQ=255)
                                                                                                                               cttcttTAGAAGCATCAGAGTTGAATTTGCGAGTCTGCTGATCACAAACACCTGTATCAATTGATGGTACCACACTAATTAATTTCTTTTTACCAGCATAATCTGCTAATGTTACTTGATTTAAGTCATTATCTAACACTg               <  1:732856/141‑1 (MQ=255)
                                                                                                                                ttcttTAGAAGCATCAGAGTTGAATTTGCGAGTCTGCTGATCACAAACACCTGTATCAATTGATGGTACCACACTAATTAATTTCTTTTTACCAGCATAATCTGCTAATGTTACTTGATTTAAGTCATTATCTAACACTGt              <  2:805963/141‑1 (MQ=255)
                                                                                                                                 tcttTAGAAGCATCAGAGTTAAATTTGCGAGTCTGCTGATCACAAACACCTGTATCAATTGATGGTACCACACTAATTAATTTCTTTTTACCAGCATAATCTGCTAATGTTACTTGATTTAAGTCATTATCTAACACTGTa             <  2:324533/141‑1 (MQ=255)
                                                                                                                                     tAGAAGCATCAGAGTTGAATTTGCGAGTCTGCTGATCACAAACACCTGTATCAATTGATGGTACCACACTAATTAATTTCTTTTTACCAGCATAATCTGCTAATGTTACTTGATTTAAGTCATTATCTAACACTGTAAAAt         >  1:1079227/1‑141 (MQ=255)
                                                                                                                                      aGAAGCATCAGAGTTGAATTTGCGAGTCTGCTGATCACGAACACCTGTATCAATTGATGGTACCACACTAATTAATTTCTTTTTACCAGCATAATCTGCTAATGTTACTTGATTTAAGTCATTATCTAACACTGTAAAATc        <  2:304075/141‑1 (MQ=255)
                                                                                                                                      aGAAGCATCAGAGTTGAATTTGCGAGTCTGCTGATCACAAACACCTGTATCAATTGATGGTACCACACTAATTAATTTCTTTTTACCAGCATAATCTGCTAATGTTACTTGATTTAAGTCATTATCTAACACTGTAAAATc        <  3:267997/141‑1 (MQ=255)
                                                                                                                                      aGAAGCATCAGAGTTGAATTTGCGAGTCTGCTGATCACAAACACCTGTATCAATTGATGGTACCACACTAATTAATTTCTTTTTACCAGCATAATCTGCTAATGTTACTTGATTTAAGTCATTATCTAACACTGTAAAATc        >  1:146766/1‑141 (MQ=255)
                                                                                                                                      aGAAGCATCAGAGTTGAATTTGCGAGTCTGCTGATCACAAACACCTGTATCAATTGATGGTACCACACTAATTAATTTCTTTTTACCAGCATAATCTGCTAATGTTACTTGATTTAAGTCATTATCTAACACTGTAAAATc        >  2:727902/1‑141 (MQ=255)
                                                                                                                                         aGCATCAGAGTTGAATTTGCGAGTCTGCTGATCACAAACATCTGTATCAATTGATGGTACCACACTAATTATTTTCTTTTTACCAGCATAATCTGCTAATGTTACTTGATTTAAGTCATTATCTAACACTGTAAAATCAgg     <  4:239625/141‑1 (MQ=255)
                                                                                                                                           cATCAGAGTTGAATTTGCGAGTCTGCTGATCACAAACACCTGTATCAATTGATGGTACCACACTAATTAATTTCTTTTTACCAGCATAATCTGCTAATGTTACTTGATTTAAGTCATTATCTAACACTGTAAAATCAGGTg   >  3:423222/1‑141 (MQ=255)
                                                                                                                                            aTCAGAGTTGAATTTGCGAGTCTGCTGATCACAAACACCTGTATCAATTGATGGTACCACACTAATTAATTTCTTTTTACCAGCATAATCTGCTAATGTTACTTGATTTAAGTCATTATCTAACACTGTAAAATCAGGTGc  <  2:385183/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                            
CCATAGTTTTCACCAAATGATAAGTCACGGTGGTCACTTAATGTAATGACATTGTCTAAACCTGCTGAAGCGCACCATCTTTTTTGTGCGAATGGTAAGTCTGCTGAAATTGTAAGCACAATCCCCTCTTCTTTAGAAGCATCAGAGTTGAATTTGCGAGTCTGCTGATCACAAACACCTGTATCAATTGATGGTACCACACTAATTAATTTCTTTTTACCAGCATAATCTGCTAATGTTACTTGATTTAAGTCATTATCTAACACTGTAAAATCAGGTGC  >  USA300TCH1516_ALE/1825430‑1825710

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: