Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 1,962,865 G→A 5.5% F91F (TTC→TTT ydeN ← Putative hydrolase YdeN

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,962,8650GA 5.5% 439.0 / 6.6 144F91F (TTC→TTTydeNPutative hydrolase YdeN
Reads supporting (aligned to +/- strand):  ref base G (55/80);  new base A (2/7);  total (57/87)
Fisher's exact test for biased strand distribution p-value = 4.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 144 total aligned reads displayed.

AAACGTATTTGTCATTTTTAGAACTCAATCCGTAAAAATTGTCAACTTTCTTTTTAATATTATCGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTGAAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGCGTACATTAATTTGTTTCGTCATAGCTTCAATCCACTGATCCAC  >  USA300TCH1516_ALE/1962726‑1963002
                                                                                                                                           |                                                                                                                                          
aaaCGTATTTGTCATTTTTAGAACTCAATCCGTAAAAATTGTCAACTTTCTTTTTAATATTATCGTAATCAATGGTTACATTACTTAAATCAGTATCTAAATCTATATTTTCTGCATCTTCTTTAACGCCCGCTATACTGa                                                                                                                                          <  4:123360/141‑1 (MQ=255)
 aaCGTATTTGTCATTTTTAGAACTCAATCCGTAAAAATTGTCAACTTTCTTTTTAATATTATCGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTGaa                                                                                                                                         >  4:2947/1‑141 (MQ=255)
   cGTATTTGTCATTTTTAGCACTCAATCCGTAAAAATTGTCAACTTTCTTTTTAATATTATCGTAATCAATGGTTACATTACTTACATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTGaaaa                                                                                                                                       <  4:301305/141‑1 (MQ=255)
     tATTTGTCATTTTTAGAACTCAATCCGTAAAAATTGTCAACTTTCTTTTTAATATTATCGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTGAAAAAg                                                                                                                                     >  4:2957/1‑141 (MQ=255)
      aTTTGTCATTTTTAGAACTCAATCCGTAAAAATTGTCAACTTTCTTTTTAATATTATCGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTGAAAAAGc                                                                                                                                    >  1:572526/1‑141 (MQ=255)
       tttGTCATTTTTAGAACTCAATCCGTAAAAATTGTCAACTTTCTTTTTAATATTATCGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTACATTTTCTGCATCTTCTTTAAAGCCCGCTATACTGAACAAGcc                                                                                                                                   >  2:14442/1‑141 (MQ=255)
        ttGTCATTTTTAGAACTCAATCCGTAAAAATTGTCAACTTTCTTTTTAATATTATCGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTGAAAAAGCCt                                                                                                                                  >  3:229094/1‑141 (MQ=255)
           tCATTTTTAGAACTCAATCCGTAAAAATTGTCAACTTTCTTTTTAATATTATCGTAATCAATGGTTACATTACTTATATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTAAAAAAGCCTTCa                                                                                                                               <  2:564068/141‑1 (MQ=255)
           tCATTTTTAGAACTCAATCCGTAAAAATTGTCAACTTTCTTTTTAATATTATCGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTGAAAAAGCCTTCa                                                                                                                               <  3:415765/141‑1 (MQ=255)
            cATTTTTAGAACTCAATCCGTAAAAATTGTCAACTTTCTTTTTAATATTATCGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTGAAAAAGCCTTCaa                                                                                                                              <  3:239847/141‑1 (MQ=255)
             aTTTTTAGAACTCAATCCGTAAAAATTGTCAACTTTCTTTTTAATATTATCGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTGAAAAAGACTTCTAt                                                                                                                             >  4:173537/1‑141 (MQ=255)
              tttttCGAACTCAACCCGTATACGTTGTCAACTTTCTTTTTAATATTATCGTAATCAATGGTTACATTACTTAAATCCACATCTAAATCTCTGTTTCCTGCACCTTCTTTCAAGCCCGCTATACTGAAAAAGCCTTCAATc                                                                                                                            <  2:918891/141‑1 (MQ=255)
              tttttAGAACTCAATCAGCAAAAACAGCCAACTTCCTTTTTAATATTATCGTAATCAATGGTTACATTACTTAAATCAATATCTTAATCTACATTCTCTGTCTCTTCTTTTACGCCCGATAAAAAAAAAAAGCCTTCAATc                                                                                                                            <  2:918822/141‑1 (MQ=255)
                tttAGAACTCAATCCGTAAAAATTGTCAACTTTCTTTTTAATATTATCGTAATCAATGGTTACATTACTTCAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTGAAAAAGCCTTCAATcag                                                                                                                          <  2:1026480/141‑3 (MQ=255)
                  tAGAACTCAATCCGTAAAAATTGTCAACTTTCTTTTTAATATTATCGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTGAAAAAGCCTTCAATCGGCt                                                                                                                        <  1:90995/141‑1 (MQ=255)
                   aGAACTCAATCCGTAAAAATTGTCAACTTTCTTTTTAATATTATCGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTGAAAAAGCCTTCAATCGGCTg                                                                                                                       >  1:256035/1‑141 (MQ=255)
                   aGAACTCAATCCGTAAAAATTGTCAACTTTCTTTTTAATATTATCGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTGAAAAAGCCTTCAATCGGCTg                                                                                                                       <  2:356836/141‑1 (MQ=255)
                    gAACTCAATCCGTAAAAATTGTCAACTTTCTTTTTAATATTATCGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTGAAAAAGCCTTCAATCGGCTGa                                                                                                                      >  3:457432/1‑141 (MQ=255)
                     aaCTCAATCCGTAAAAATTGTCAACTTTCTTTTTAATATTATCGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTGAAAAAGCCTTCAATCGGCTGat                                                                                                                     <  2:604623/141‑1 (MQ=255)
                     aaCTCAATCCGTAAAAATTGTCAACTTTCTTTTTAATATTATCGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGAAAAAAAAAAAAAAAAAGCCTTCAATCGGCTGat                                                                                                                     <  2:460431/141‑1 (MQ=255)
                      aCTCAATCCGTAAAAATTGTCAACTTTCTTTTTAATATTATCGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTGAAAAAGCCTTCAATCGGCTGatc                                                                                                                    >  3:38352/1‑141 (MQ=255)
                       cTCAATCCGTAAAAATTGTCAACTTTCTTTTTAATATTATCGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTGAAAAAGCCTTCAATCGGCTGatca                                                                                                                   >  1:411956/1‑141 (MQ=255)
                        tCAATCCGTAAAAATTGTCAACTTTCTTTTTAATATTATCGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTGAAAAAGCCTTCAATCGGCTGatcaa                                                                                                                  >  1:56144/1‑141 (MQ=255)
                        tCAATCCGTAAAAATTGTCAACTTTCTTTTTAATATTATCGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTGAAAAAGCCTTCAATCGGCTGatcaa                                                                                                                  >  3:395067/1‑141 (MQ=255)
                                    aaTTGTCATCTTTCTTTTTAATATTATCGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTGAAAAAGCCTTCAATCGGCTGATCAATCATTTCAatat                                                                                                      <  2:351257/141‑1 (MQ=255)
                                    aaTTGTCAACTTTCTTTTTAATATTATCGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTGAAAAAGCCTTCAATCGGCTGATCAATCATTTCAatat                                                                                                      <  3:267116/141‑1 (MQ=255)
                                    aaTTGTCAACTTTCTTTTTAATATTATCGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTGAAAAAGCCTTCAATCGGCTGATCAATCATTTCAatat                                                                                                      <  2:959912/141‑1 (MQ=255)
                                    aaTTGTCAACTTTCTTTTTAATATTATCGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTGAAAAAGCCTTCAATCGGCTGATCAATCATTTCAatat                                                                                                      <  1:1014590/141‑1 (MQ=255)
                                      ttGTCAACTTTCTTTTTAATATTATCGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTGAAAAAGCCTTCAATCGGCTGATCAATCATTTCAatatat                                                                                                    >  1:678471/1‑141 (MQ=255)
                                      ttGTCAACTTTCTTTTTAATATTATCGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTGAAAAAGCCTTCAATCGGCTGATCAATCATTTCAatatat                                                                                                    <  1:421665/141‑1 (MQ=255)
                                       tGTCAACTTTCTTTTTTTTTTTATTTTAATCAAAGGTAACAATACTTAATTTAATATCTAAATCTATATTCTGTGCTTCTTCTTTAAAGCCCGCTATACTGAAAAAGACTTCATTCGTCTGATCATTCATTTCAATATAtt                                                                                                   >  4:418902/1‑141 (MQ=255)
                                        gTCAACTTTCTTTTTAATATTATCGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTGAAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATAttt                                                                                                  >  4:455832/1‑141 (MQ=255)
                                            aCTTTCTTTTTAATATTATCGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTGAAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTaaa                                                                                              <  1:554509/141‑1 (MQ=255)
                                              tttctttTTAATATTATCGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTGAAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGc                                                                                            <  3:299652/141‑1 (MQ=255)
                                               ttctttTTAATATTATCGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTGAAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCt                                                                                           <  2:608098/141‑1 (MQ=255)
                                                 ctttTTAATATTATCGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTGAAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCtgt                                                                                         <  2:5554/141‑1 (MQ=255)
                                                      tAATATTATCGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTGAAAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGAtt                                                                                     <  2:853575/141‑1 (MQ=255)
                                                       aaTATTATCGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTGAAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGa                                                                                   >  2:222758/1‑141 (MQ=255)
                                                        atatTATCGTAATCAATGGTTACATTAGTTAAATCAATATCTAAATATATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTGAAAAAGACTTCAATCGGCTGATCAATCATTTCAATATATTTTAAATCTGTGATTgca                                                                                  >  2:1066934/1‑139 (MQ=255)
                                                        atatTATCGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTGAAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGaa                                                                                  <  1:1033617/141‑1 (MQ=255)
                                                         tattatCGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTGAAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAAc                                                                                 <  4:395067/141‑1 (MQ=255)
                                                           ttatCGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTGAAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCt                                                                               <  3:258398/141‑1 (MQ=255)
                                                            tatCGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTAAAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTa                                                                              <  3:426372/141‑1 (MQ=255)
                                                               cGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTGAAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAAc                                                                           >  1:329362/1‑141 (MQ=255)
                                                                     cAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTGAAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCAtgtg                                                                     <  1:543689/141‑1 (MQ=255)
                                                                     cAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTGAAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCAtgtg                                                                     <  1:770214/141‑1 (MQ=255)
                                                                      aaTGTTTACATTACTGAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTGAAAAAGCCTTCAATCAGCTTATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATgtgt                                                                    <  1:247420/141‑1 (MQ=255)
                                                                      aaTGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTGAAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATgtgt                                                                    <  1:652293/141‑1 (MQ=255)
                                                                       aTGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTGAAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGtt                                                                   <  3:288903/141‑1 (MQ=255)
                                                                           ttACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTGAAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACaa                                                               >  1:760349/1‑141 (MQ=255)
                                                                           ttACATTACTGAAATCAATACCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCAAAAAAACAGAAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACaa                                                               <  4:340505/141‑1 (MQ=255)
                                                                                ttACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTGAAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAatat                                                          <  2:752938/141‑1 (MQ=255)
                                                                                  aCTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTGAAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGt                                                        <  4:245558/141‑1 (MQ=255)
                                                                                  aCTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTGAAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGt                                                        <  2:817785/141‑1 (MQ=255)
                                                                                    ttAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTGAAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTAt                                                      <  1:443978/141‑1 (MQ=255)
                                                                                    ttAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTGAAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTAt                                                      <  1:363907/141‑1 (MQ=255)
                                                                                    ttAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCAACAAAACAAAAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTAt                                                      <  2:752115/141‑1 (MQ=255)
                                                                                       aaTCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTGAAAAAGCCTTCAATCGGCTGATCAAAAATTTCCCTATATTTTCAAGATGTGATTGAACATAAACCATGTGTTACAAAATATGTATCCt                                                   >  1:99045/1‑141 (MQ=255)
                                                                                            atatCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTGAAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCtttttt                                              >  2:1063980/1‑140 (MQ=255)
                                                                                             tatCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTGAAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGc                                             >  1:1046376/1‑141 (MQ=255)
                                                                                              atCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTGAAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGCg                                            <  1:173236/141‑1 (MQ=255)
                                                                                                  aaaTCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTGAAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGCGTACa                                        <  3:124373/141‑1 (MQ=255)
                                                                                                  aaaTCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTGAAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGCGTACa                                        >  2:6569/1‑141 (MQ=255)
                                                                                                   aaTCTATATTTTCTGTATCTTCTTTAAAGCCCGCTATAAAAAAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGCGTACAt                                       <  2:474732/141‑1 (MQ=255)
                                                                                                    aTCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTGAAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGCGTACAtt                                      >  1:699309/1‑141 (MQ=255)
                                                                                                       tatgTTTTCTGCATCTTCTGTAAAGCCCGCTATACTGAAAAAGCCTTCAATCGGATGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGCGTACATTAAt                                   <  2:785858/141‑1 (MQ=255)
                                                                                                       tataTTTTCTGCATCTTCTTTAAAGCCCGCTATACTGAAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGCGTACATTAAt                                   <  2:835186/141‑1 (MQ=255)
                                                                                                       tataTTTTCTGCATCTTCTTTAAAGCCCGCTATACTGAAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGCGTACATTAAt                                   >  1:323810/1‑141 (MQ=255)
                                                                                                       tataTTTTCTGCATCTTCTTTAAAGCCCGCTATACTGAAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAAAAAAAAAAAAAAAAATATGTATCCTTTTTGCGTACATTAAt                                   <  4:26680/141‑1 (MQ=255)
                                                                                                        atatTTTCTGCATCTTCTTTAAAGCCCGCTATACTGAAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGCGTACATTAAtt                                  <  1:383206/141‑1 (MQ=255)
                                                                                                         tatTTTCTGCATCTTCTTTAAAGCCCGCTATACTGAAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGCGTACATTAAttt                                 >  4:190297/1‑141 (MQ=255)
                                                                                                          atTTTCTGCATCTTCTTTAAAGCCCGCTATACTGAAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGCGTACATTAATTTg                                >  1:295073/1‑141 (MQ=255)
                                                                                                            tttCTGCATCTTCTTTAAAGCCCGCTATACTGAAAAAGCCTTCAATTGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGCGTACATTAATTTGtt                              >  3:377140/1‑141 (MQ=255)
                                                                                                               cTGCATCTTCTTTAAAGCCCGCTATACTGAAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGCGTACATTAATTTGTTTCg                           >  3:413473/1‑141 (MQ=255)
                                                                                                                 gCATCTTCTTTAAAGCCCGCTATACTGAAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGCGTACATTAATTTGTTTCGTc                         <  4:307990/141‑1 (MQ=255)
                                                                                                                 gCATCTTCTTTAAAGCCCGCTATACTGAAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGCGTACATTAATTTGTTTCGTc                         <  2:982022/141‑1 (MQ=255)
                                                                                                                    tcttctTTAAAGCCCGCTATACTGAAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTTCGTACATTAATTTGTTTCGTCATa                      >  2:13737/1‑141 (MQ=255)
                                                                                                                     cttcttTAAAGCCCGCTATACTGAAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGCGTACATTAATTTGTTTCGTCATAg                     >  2:716114/1‑141 (MQ=255)
                                                                                                                       tcttTAAAGCCCGCTATACTGAAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGCTTACATTAATTTGTTTCGTCATAGCt                   <  2:223406/141‑1 (MQ=255)
                                                                                                                       tcttTAAAGCCCGCTATACTGAAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGCGTACATTAATTTGTTTCGTCATAGCt                   <  4:5739/141‑1 (MQ=255)
                                                                                                                           tAAAGCCCGCTATACTGAAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGCGTACATTAATTTGTTTCGTCATAGCTTCaa               >  3:194086/1‑141 (MQ=255)
                                                                                                                            aaaGCCCGCTATACTGAAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGCGTACATTAATTTGTTTCGTCATAGCTTCAat              >  2:707076/1‑141 (MQ=255)
                                                                                                                             aaGCCCGCTATACTGAAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGCGTACATTAATTTGTTTCGTCATAGCTTCAatc             >  1:842690/1‑141 (MQ=255)
                                                                                                                              accccGCTATACTGAAAAAGCCTTCAATCGGCTGATCAATCATTTCTATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGCATACATTAATGTGTTGCGGCATAGCTTCAatcc            >  4:108147/3‑141 (MQ=255)
                                                                                                                               gCCCGCTATACTGAAAAAGCCTTCACTCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGCGTACATTAATTTGTTTCGTCATAGCTTCAatcca           <  2:348401/141‑1 (MQ=255)
                                                                                                                               gCCCGCTATACTGAAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGCGTACATTAATTTGTTTCGTCATAGCTTCAatcca           >  2:323973/1‑141 (MQ=255)
                                                                                                                               gCCCGCTATACTGAAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGCGTACATTAATTTGTTTCGTCATAGCTTCAatcca           <  2:348583/141‑1 (MQ=255)
                                                                                                                                 ccGCTATACTGAAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGCGTACATTAATTTGTTTCGTCATAGCTTCAatccact         >  1:690931/1‑141 (MQ=255)
                                                                                                                                 ccGCTATACTGAAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGCGTACATTAATTTGTTTCGTCATAGCTTCAatccact         >  1:471702/1‑141 (MQ=255)
                                                                                                                                  cGCTATACTAAAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGCGTACATTAATTTGTTTCGTCATAGCTTCAatccactg        >  3:170367/1‑141 (MQ=255)
                                                                                                                                   gCTATACTGAAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTaa                                                                                               >  2:670347/1‑53 (MQ=255)
                                                                                                                                   gCTATACTGAAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTaa                                                                                               <  1:670347/53‑1 (MQ=255)
                                                                                                                                   gCTATACTGAAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGCGTACATTAATTTGTTTCGTCATAGCTTCAatccactga       <  2:876459/141‑1 (MQ=255)
                                                                                                                                   gCTATACTGAAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGCGTACATTAATTTGTTTCGTCATAGCTTCAatccactga       <  2:116478/141‑1 (MQ=255)
                                                                                                                                   gCTATACTGAAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGCGTACATTAATTTGTTTCGTCATAGCTTCAatccactga       <  2:283973/141‑1 (MQ=255)
                                                                                                                                     tataCTGAAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGCGTACATTAATTTGTTTCGTCATAGCTTCAatccactgatc     <  2:289139/141‑1 (MQ=255)
                                                                                                                                     tataCTGAAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGCGTACATTAATTTGTTTCGTCATAGCTTCAatccactgatc     >  1:177950/1‑141 (MQ=255)
                                                                                                                                      ataCTGAAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGTGTACATTAATTTGTTTCGTCATAGCTTCAatccactgatcc    <  3:2957/141‑1 (MQ=255)
                                                                                                                                      ataCTGAAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGCGTACATTAATTTGTTTCGTCATAGCTTCAatccactgatcc    <  1:902032/141‑1 (MQ=255)
                                                                                                                                        aCTGAAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGCGTACATTAATTTGTTTCGTCATAGCTTCAatccactgatccac  <  2:525102/141‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                          
AAACGTATTTGTCATTTTTAGAACTCAATCCGTAAAAATTGTCAACTTTCTTTTTAATATTATCGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTGAAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGCGTACATTAATTTGTTTCGTCATAGCTTCAATCCACTGATCCAC  >  USA300TCH1516_ALE/1962726‑1963002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: