BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	1,962,865	G→A	5.5%	F91F (TTC→TTT)	ydeN ←	Putative hydrolase YdeN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,962,865	0	G	A	5.5%	439.0 / 6.6	144	F91F (TTC→TTT)	ydeN	Putative hydrolase YdeN
Reads supporting (aligned to +/- strand): ref base G (55/80); new base A (2/7); total (57/87)
Fisher's exact test for biased strand distribution p-value = 4.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 144 total aligned reads displayed.

AAACGTATTTGTCATTTTTAGAACTCAATCCGTAAAAATTGTCAACTTTCTTTTTAATATTATCGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTG‑AAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGCGTACATTAATTTGTTTCGTCATAGCTTCAATCCACTGATCCAC  >  USA300TCH1516_ALE/1962726‑1963002

aaaCGTATTTGTCATTTTTAGAACTCAATCCGTAAAAATTGTCAACTTTCTTTTTAATATTATCGTAATCAATGGTTACATTACTTAAATCAGTATCTAAATCTATATTTTCTGCATCTTCTTTAACGCCCGCTATACTG‑a                                                                                                                                          <  4:123360/141‑1 (MQ=255)

 aaCGTATTTGTCATTTTTAGAACTCAATCCGTAAAAATTGTCAACTTTCTTTTTAATATTATCGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTG‑aa                                                                                                                                         >  4:2947/1‑141 (MQ=255)

   cGTATTTGTCATTTTTAGCACTCAATCCGTAAAAATTGTCAACTTTCTTTTTAATATTATCGTAATCAATGGTTACATTACTTACATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTG‑aaaa                                                                                                                                       <  4:301305/141‑1 (MQ=255)

     tATTTGTCATTTTTAGAACTCAATCCGTAAAAATTGTCAACTTTCTTTTTAATATTATCGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTG‑AAAAAg                                                                                                                                     >  4:2957/1‑141 (MQ=255)

      aTTTGTCATTTTTAGAACTCAATCCGTAAAAATTGTCAACTTTCTTTTTAATATTATCGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTG‑AAAAAGc                                                                                                                                    >  1:572526/1‑141 (MQ=255)

       tttGTCATTTTTAGAACTCAATCCGTAAAAATTGTCAACTTTCTTTTTAATATTATCGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTACATTTTCTGCATCTTCTTTAAAGCCCGCTATACTG‑AACAAGcc                                                                                                                                   >  2:14442/1‑141 (MQ=255)

        ttGTCATTTTTAGAACTCAATCCGTAAAAATTGTCAACTTTCTTTTTAATATTATCGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTG‑AAAAAGCCt                                                                                                                                  >  3:229094/1‑141 (MQ=255)

           tCATTTTTAGAACTCAATCCGTAAAAATTGTCAACTTTCTTTTTAATATTATCGTAATCAATGGTTACATTACTTATATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTA‑AAAAAGCCTTCa                                                                                                                               <  2:564068/141‑1 (MQ=255)

           tCATTTTTAGAACTCAATCCGTAAAAATTGTCAACTTTCTTTTTAATATTATCGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTG‑AAAAAGCCTTCa                                                                                                                               <  3:415765/141‑1 (MQ=255)

            cATTTTTAGAACTCAATCCGTAAAAATTGTCAACTTTCTTTTTAATATTATCGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTG‑AAAAAGCCTTCaa                                                                                                                              <  3:239847/141‑1 (MQ=255)

             aTTTTTAGAACTCAATCCGTAAAAATTGTCAACTTTCTTTTTAATATTATCGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTG‑AAAAAGACTTCTAt                                                                                                                             >  4:173537/1‑141 (MQ=255)

              tttttCGAACTCAACCCGTATACGTTGTCAACTTTCTTTTTAATATTATCGTAATCAATGGTTACATTACTTAAATCCACATCTAAATCTCTGTTTCCTGCACCTTCTTTCAAGCCCGCTATACTG‑AAAAAGCCTTCAATc                                                                                                                            <  2:918891/141‑1 (MQ=255)

              tttttAGAACTCAATCAGCAAAAACAGCCAACTTCCTTTTTAATATTATCGTAATCAATGGTTACATTACTTAAATCAATATCTTAATCTACATTCTCTGTCTCTTCTTTTACGCCCGATAAAAAA‑AAAAAGCCTTCAATc                                                                                                                            <  2:918822/141‑1 (MQ=255)

                tttAGAACTCAATCCGTAAAAATTGTCAACTTTCTTTTTAATATTATCGTAATCAATGGTTACATTACTTCAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTG‑AAAAAGCCTTCAATcag                                                                                                                          <  2:1026480/141‑3 (MQ=255)

                  tAGAACTCAATCCGTAAAAATTGTCAACTTTCTTTTTAATATTATCGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTG‑AAAAAGCCTTCAATCGGCt                                                                                                                        <  1:90995/141‑1 (MQ=255)

                   aGAACTCAATCCGTAAAAATTGTCAACTTTCTTTTTAATATTATCGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTG‑AAAAAGCCTTCAATCGGCTg                                                                                                                       >  1:256035/1‑141 (MQ=255)

                   aGAACTCAATCCGTAAAAATTGTCAACTTTCTTTTTAATATTATCGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTG‑AAAAAGCCTTCAATCGGCTg                                                                                                                       <  2:356836/141‑1 (MQ=255)

                    gAACTCAATCCGTAAAAATTGTCAACTTTCTTTTTAATATTATCGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTG‑AAAAAGCCTTCAATCGGCTGa                                                                                                                      >  3:457432/1‑141 (MQ=255)

                     aaCTCAATCCGTAAAAATTGTCAACTTTCTTTTTAATATTATCGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTG‑AAAAAGCCTTCAATCGGCTGat                                                                                                                     <  2:604623/141‑1 (MQ=255)

                     aaCTCAATCCGTAAAAATTGTCAACTTTCTTTTTAATATTATCGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGAAAAAAAAAAAA‑AAAAAGCCTTCAATCGGCTGat                                                                                                                     <  2:460431/141‑1 (MQ=255)

                      aCTCAATCCGTAAAAATTGTCAACTTTCTTTTTAATATTATCGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTG‑AAAAAGCCTTCAATCGGCTGatc                                                                                                                    >  3:38352/1‑141 (MQ=255)

                       cTCAATCCGTAAAAATTGTCAACTTTCTTTTTAATATTATCGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTG‑AAAAAGCCTTCAATCGGCTGatca                                                                                                                   >  1:411956/1‑141 (MQ=255)

                        tCAATCCGTAAAAATTGTCAACTTTCTTTTTAATATTATCGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTG‑AAAAAGCCTTCAATCGGCTGatcaa                                                                                                                  >  1:56144/1‑141 (MQ=255)

                        tCAATCCGTAAAAATTGTCAACTTTCTTTTTAATATTATCGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTG‑AAAAAGCCTTCAATCGGCTGatcaa                                                                                                                  >  3:395067/1‑141 (MQ=255)

                                    aaTTGTCATCTTTCTTTTTAATATTATCGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTG‑AAAAAGCCTTCAATCGGCTGATCAATCATTTCAatat                                                                                                      <  2:351257/141‑1 (MQ=255)

                                    aaTTGTCAACTTTCTTTTTAATATTATCGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTG‑AAAAAGCCTTCAATCGGCTGATCAATCATTTCAatat                                                                                                      <  3:267116/141‑1 (MQ=255)

                                    aaTTGTCAACTTTCTTTTTAATATTATCGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTG‑AAAAAGCCTTCAATCGGCTGATCAATCATTTCAatat                                                                                                      <  2:959912/141‑1 (MQ=255)

                                    aaTTGTCAACTTTCTTTTTAATATTATCGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTG‑AAAAAGCCTTCAATCGGCTGATCAATCATTTCAatat                                                                                                      <  1:1014590/141‑1 (MQ=255)

                                      ttGTCAACTTTCTTTTTAATATTATCGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTG‑AAAAAGCCTTCAATCGGCTGATCAATCATTTCAatatat                                                                                                    >  1:678471/1‑141 (MQ=255)

                                      ttGTCAACTTTCTTTTTAATATTATCGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTG‑AAAAAGCCTTCAATCGGCTGATCAATCATTTCAatatat                                                                                                    <  1:421665/141‑1 (MQ=255)

                                       tGTCAACTTTCTTTTTTTTTTTATTTTAATCAAAGGTAACAATACTTAATTTAATATCTAAATCTATATTCTGTGCTTCTTCTTTAAAGCCCGCTATACTG‑AAAAAGACTTCATTCGTCTGATCATTCATTTCAATATAtt                                                                                                   >  4:418902/1‑141 (MQ=255)

                                        gTCAACTTTCTTTTTAATATTATCGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTG‑AAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATAttt                                                                                                  >  4:455832/1‑141 (MQ=255)

                                            aCTTTCTTTTTAATATTATCGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTG‑AAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTaaa                                                                                              <  1:554509/141‑1 (MQ=255)

                                              tttctttTTAATATTATCGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTG‑AAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGc                                                                                            <  3:299652/141‑1 (MQ=255)

                                               ttctttTTAATATTATCGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTG‑AAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCt                                                                                           <  2:608098/141‑1 (MQ=255)

                                                 ctttTTAATATTATCGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTG‑AAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCtgt                                                                                         <  2:5554/141‑1 (MQ=255)

                                                      tAATATTATCGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTGAAAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGAtt                                                                                     <  2:853575/141‑1 (MQ=255)

                                                       aaTATTATCGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTG‑AAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGa                                                                                   >  2:222758/1‑141 (MQ=255)

                                                        atatTATCGTAATCAATGGTTACATTAGTTAAATCAATATCTAAATATATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTG‑AAAAAGACTTCAATCGGCTGATCAATCATTTCAATATATTTTAAATCTGTGATTgca                                                                                  >  2:1066934/1‑139 (MQ=255)

                                                        atatTATCGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTG‑AAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGaa                                                                                  <  1:1033617/141‑1 (MQ=255)

                                                         tattatCGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTG‑AAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAAc                                                                                 <  4:395067/141‑1 (MQ=255)

                                                           ttatCGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTG‑AAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCt                                                                               <  3:258398/141‑1 (MQ=255)

                                                            tatCGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTA‑AAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTa                                                                              <  3:426372/141‑1 (MQ=255)

                                                               cGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTG‑AAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAAc                                                                           >  1:329362/1‑141 (MQ=255)

                                                                     cAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTG‑AAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCAtgtg                                                                     <  1:543689/141‑1 (MQ=255)

                                                                     cAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTG‑AAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCAtgtg                                                                     <  1:770214/141‑1 (MQ=255)

                                                                      aaTGTTTACATTACTGAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTG‑AAAAAGCCTTCAATCAGCTTATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATgtgt                                                                    <  1:247420/141‑1 (MQ=255)

                                                                      aaTGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTG‑AAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATgtgt                                                                    <  1:652293/141‑1 (MQ=255)

                                                                       aTGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTG‑AAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGtt                                                                   <  3:288903/141‑1 (MQ=255)

                                                                           ttACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTG‑AAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACaa                                                               >  1:760349/1‑141 (MQ=255)

                                                                           ttACATTACTGAAATCAATACCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCAAAAAAACAG‑AAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACaa                                                               <  4:340505/141‑1 (MQ=255)

                                                                                ttACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTG‑AAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAatat                                                          <  2:752938/141‑1 (MQ=255)

                                                                                  aCTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTG‑AAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGt                                                        <  4:245558/141‑1 (MQ=255)

                                                                                  aCTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTG‑AAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGt                                                        <  2:817785/141‑1 (MQ=255)

                                                                                    ttAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTG‑AAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTAt                                                      <  1:443978/141‑1 (MQ=255)

                                                                                    ttAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTG‑AAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTAt                                                      <  1:363907/141‑1 (MQ=255)

                                                                                    ttAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCAACAAAACAA‑AAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTAt                                                      <  2:752115/141‑1 (MQ=255)

                                                                                       aaTCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTG‑AAAAAGCCTTCAATCGGCTGATCAAAAATTTCCCTATATTTTCAAGATGTGATTGAACATAAACCATGTGTTACAAAATATGTATCCt                                                   >  1:99045/1‑141 (MQ=255)

                                                                                            atatCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTG‑AAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCtttttt                                              >  2:1063980/1‑140 (MQ=255)

                                                                                             tatCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTG‑AAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGc                                             >  1:1046376/1‑141 (MQ=255)

                                                                                              atCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTG‑AAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGCg                                            <  1:173236/141‑1 (MQ=255)

                                                                                                  aaaTCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTG‑AAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGCGTACa                                        <  3:124373/141‑1 (MQ=255)

                                                                                                  aaaTCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTG‑AAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGCGTACa                                        >  2:6569/1‑141 (MQ=255)

                                                                                                   aaTCTATATTTTCTGTATCTTCTTTAAAGCCCGCTATAAAA‑AAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGCGTACAt                                       <  2:474732/141‑1 (MQ=255)

                                                                                                    aTCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTG‑AAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGCGTACAtt                                      >  1:699309/1‑141 (MQ=255)

                                                                                                       tatgTTTTCTGCATCTTCTGTAAAGCCCGCTATACTG‑AAAAAGCCTTCAATCGGATGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGCGTACATTAAt                                   <  2:785858/141‑1 (MQ=255)

                                                                                                       tataTTTTCTGCATCTTCTTTAAAGCCCGCTATACTG‑AAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGCGTACATTAAt                                   <  2:835186/141‑1 (MQ=255)

                                                                                                       tataTTTTCTGCATCTTCTTTAAAGCCCGCTATACTG‑AAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGCGTACATTAAt                                   >  1:323810/1‑141 (MQ=255)

                                                                                                       tataTTTTCTGCATCTTCTTTAAAGCCCGCTATACTG‑AAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAAAAAAAAAAAAAAAAATATGTATCCTTTTTGCGTACATTAAt                                   <  4:26680/141‑1 (MQ=255)

                                                                                                        atatTTTCTGCATCTTCTTTAAAGCCCGCTATACTG‑AAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGCGTACATTAAtt                                  <  1:383206/141‑1 (MQ=255)

                                                                                                         tatTTTCTGCATCTTCTTTAAAGCCCGCTATACTG‑AAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGCGTACATTAAttt                                 >  4:190297/1‑141 (MQ=255)

                                                                                                          atTTTCTGCATCTTCTTTAAAGCCCGCTATACTG‑AAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGCGTACATTAATTTg                                >  1:295073/1‑141 (MQ=255)

                                                                                                            tttCTGCATCTTCTTTAAAGCCCGCTATACTG‑AAAAAGCCTTCAATTGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGCGTACATTAATTTGtt                              >  3:377140/1‑141 (MQ=255)

                                                                                                               cTGCATCTTCTTTAAAGCCCGCTATACTG‑AAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGCGTACATTAATTTGTTTCg                           >  3:413473/1‑141 (MQ=255)

                                                                                                                 gCATCTTCTTTAAAGCCCGCTATACTG‑AAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGCGTACATTAATTTGTTTCGTc                         <  4:307990/141‑1 (MQ=255)

                                                                                                                 gCATCTTCTTTAAAGCCCGCTATACTG‑AAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGCGTACATTAATTTGTTTCGTc                         <  2:982022/141‑1 (MQ=255)

                                                                                                                    tcttctTTAAAGCCCGCTATACTG‑AAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTTCGTACATTAATTTGTTTCGTCATa                      >  2:13737/1‑141 (MQ=255)

                                                                                                                     cttcttTAAAGCCCGCTATACTG‑AAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGCGTACATTAATTTGTTTCGTCATAg                     >  2:716114/1‑141 (MQ=255)

                                                                                                                       tcttTAAAGCCCGCTATACTG‑AAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGCTTACATTAATTTGTTTCGTCATAGCt                   <  2:223406/141‑1 (MQ=255)

                                                                                                                       tcttTAAAGCCCGCTATACTG‑AAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGCGTACATTAATTTGTTTCGTCATAGCt                   <  4:5739/141‑1 (MQ=255)

                                                                                                                           tAAAGCCCGCTATACTG‑AAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGCGTACATTAATTTGTTTCGTCATAGCTTCaa               >  3:194086/1‑141 (MQ=255)

                                                                                                                            aaaGCCCGCTATACTG‑AAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGCGTACATTAATTTGTTTCGTCATAGCTTCAat              >  2:707076/1‑141 (MQ=255)

                                                                                                                             aaGCCCGCTATACTG‑AAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGCGTACATTAATTTGTTTCGTCATAGCTTCAatc             >  1:842690/1‑141 (MQ=255)

                                                                                                                              accccGCTATACTG‑AAAAAGCCTTCAATCGGCTGATCAATCATTTCTATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGCATACATTAATGTGTTGCGGCATAGCTTCAatcc            >  4:108147/3‑141 (MQ=255)

                                                                                                                               gCCCGCTATACTG‑AAAAAGCCTTCACTCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGCGTACATTAATTTGTTTCGTCATAGCTTCAatcca           <  2:348401/141‑1 (MQ=255)

                                                                                                                               gCCCGCTATACTG‑AAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGCGTACATTAATTTGTTTCGTCATAGCTTCAatcca           >  2:323973/1‑141 (MQ=255)

                                                                                                                               gCCCGCTATACTG‑AAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGCGTACATTAATTTGTTTCGTCATAGCTTCAatcca           <  2:348583/141‑1 (MQ=255)

                                                                                                                                 ccGCTATACTG‑AAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGCGTACATTAATTTGTTTCGTCATAGCTTCAatccact         >  1:690931/1‑141 (MQ=255)

                                                                                                                                 ccGCTATACTG‑AAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGCGTACATTAATTTGTTTCGTCATAGCTTCAatccact         >  1:471702/1‑141 (MQ=255)

                                                                                                                                  cGCTATACTA‑AAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGCGTACATTAATTTGTTTCGTCATAGCTTCAatccactg        >  3:170367/1‑141 (MQ=255)

                                                                                                                                   gCTATACTG‑AAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTaa                                                                                               >  2:670347/1‑53 (MQ=255)

                                                                                                                                   gCTATACTG‑AAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTaa                                                                                               <  1:670347/53‑1 (MQ=255)

                                                                                                                                   gCTATACTG‑AAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGCGTACATTAATTTGTTTCGTCATAGCTTCAatccactga       <  2:876459/141‑1 (MQ=255)

                                                                                                                                   gCTATACTG‑AAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGCGTACATTAATTTGTTTCGTCATAGCTTCAatccactga       <  2:116478/141‑1 (MQ=255)

                                                                                                                                   gCTATACTG‑AAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGCGTACATTAATTTGTTTCGTCATAGCTTCAatccactga       <  2:283973/141‑1 (MQ=255)

                                                                                                                                     tataCTG‑AAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGCGTACATTAATTTGTTTCGTCATAGCTTCAatccactgatc     <  2:289139/141‑1 (MQ=255)

                                                                                                                                     tataCTG‑AAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGCGTACATTAATTTGTTTCGTCATAGCTTCAatccactgatc     >  1:177950/1‑141 (MQ=255)

                                                                                                                                      ataCTG‑AAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGTGTACATTAATTTGTTTCGTCATAGCTTCAatccactgatcc    <  3:2957/141‑1 (MQ=255)

                                                                                                                                      ataCTG‑AAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGCGTACATTAATTTGTTTCGTCATAGCTTCAatccactgatcc    <  1:902032/141‑1 (MQ=255)

                                                                                                                                        aCTG‑AAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGCGTACATTAATTTGTTTCGTCATAGCTTCAatccactgatccac  <  2:525102/141‑1 (MQ=255)

AAACGTATTTGTCATTTTTAGAACTCAATCCGTAAAAATTGTCAACTTTCTTTTTAATATTATCGTAATCAATGGTTACATTACTTAAATCAATATCTAAATCTATATTTTCTGCATCTTCTTTAAAGCCCGCTATACTG‑AAAAAGCCTTCAATCGGCTGATCAATCATTTCAATATATTTTAAAGCTGTGATTGAACCTAAACCATGTGTTACAAAATATGTATCCTTTTTGCGTACATTAATTTGTTTCGTCATAGCTTCAATCCACTGATCCAC  >  USA300TCH1516_ALE/1962726‑1963002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`