BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	2,027,805	C→A	5.7%	V48F (GTT→TTT)	vraS ←	Sensor protein VraS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,027,805	0	C	A	5.7%	389.8 / 7.9	114	V48F (GTT→TTT)	vraS	Sensor protein VraS
Reads supporting (aligned to +/- strand): ref base C (47/60); new base A (5/2); total (52/62)
Fisher's exact test for biased strand distribution p-value = 2.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 116 total aligned reads displayed.

ATTAATGCCAACTGTTTCGCCTTCCATTGAACGCTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAGAAATGCAGCTAGCATGCTATATACTAAGATGAGCATTGAACCAATTGTTCTAATGTAGTGGTTCA  >  USA300TCH1516_ALE/2027665‑2027945

 ttAATGCCAACTGTTTCGCCTTCCATTGAACGCTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACt                                                                                                                                             >  2:255100/1‑141 (MQ=255)

 ttAATGCCAACTGTTTCGCCTTCCATTGAACGCTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATATAAAAATAAAAAAACt                                                                                                                                             >  2:949887/1‑141 (MQ=255)

      gCCAACTGTTTCGCCTTCCATTGAACGCTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTAt                                                                                                                                        >  3:375236/1‑141 (MQ=255)

      gCCAACTGTTTCGCCTTCCATTGAACGCTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTAt                                                                                                                                        >  2:779385/1‑141 (MQ=255)

      gCCAACTGTTTCGCCTTCCATTGAACGCTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAAATGGTAt                                                                                                                                        >  4:229532/1‑141 (MQ=255)

       ccAACTGTTTCGCCTTCCATTGATCGCTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAAATGGTAtt                                                                                                                                       <  1:246224/141‑1 (MQ=255)

       ccAACTGTTTCGCCTTCCATTGAACGCTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTAtt                                                                                                                                       <  1:191874/141‑1 (MQ=255)

        cAACTGTTTCGCCTTCCATTGAACGCTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTc                                                                                                                                      <  3:106684/141‑1 (MQ=255)

         aaCTGTTTCGCCTTCCATTGAACGCTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTcc                                                                                                                                     >  2:671930/1‑141 (MQ=255)

         aaCTGTTTCGCCTTCCATTGAACGCTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTcc                                                                                                                                     >  1:697763/1‑141 (MQ=255)

         aaCTGTTTCGCCTTCCATTGAACGCTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTcc                                                                                                                                     >  3:339410/1‑141 (MQ=255)

          aCTGTTTCGCCTTCCATTGAACGCTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCg                                                                                                                                    <  1:594073/141‑1 (MQ=255)

             gTTTCGCCTTCCATTGAACGCTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAat                                                                                                                                 <  4:23621/141‑1 (MQ=255)

              tttCGCCTTCCATTGAACGCTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAata                                                                                                                                >  3:362369/1‑141 (MQ=255)

              tttCGCCTTCCATTGAACGCTCAATTTGCGGCTTAATCCAATCATTTTGATGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAata                                                                                                                                <  4:307204/141‑1 (MQ=255)

               ttCGCCTTCCATTGAACGCTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAatat                                                                                                                               >  3:382408/1‑141 (MQ=255)

                  gCCTTCCATTGAACGCTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACACTAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATAtttt                                                                                                                            >  2:315490/1‑140 (MQ=255)

                   ccTTCCATTGAACGCTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTtgt                                                                                                                           >  2:531283/1‑141 (MQ=255)

                      tCCATTGAACTCTCTATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGta                                                                                                                        <  2:209648/141‑1 (MQ=255)

                      tCCATTGAACGCTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGta                                                                                                                        <  2:4376/141‑1 (MQ=255)

                       ccATTGAACGCTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGtat                                                                                                                       >  2:792338/1‑141 (MQ=255)

                       ccATTGAACGCTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGtat                                                                                                                       >  2:105543/1‑141 (MQ=255)

                        cATTGAACGCTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGtata                                                                                                                      <  1:579718/141‑1 (MQ=255)

                        cATTGAACGCTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGtata                                                                                                                      <  1:830184/141‑1 (MQ=255)

                        cATTGAACGCTCAATTTGCGTCTTAATCCAATCACTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGtata                                                                                                                      <  1:1007105/141‑1 (MQ=255)

                          ttGAACGCTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATACTACACAATAATATGATGATGCGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATaaa                                                                                                                    >  2:1039030/1‑141 (MQ=255)

                          ttGAACGCTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATaaa                                                                                                                    >  2:393225/1‑141 (MQ=255)

                           tGAACGCTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATaaaa                                                                                                                   >  1:529203/1‑141 (MQ=255)

                             aaCGCTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACa                                                                                                                 <  3:221712/141‑1 (MQ=255)

                             aaCGCTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACa                                                                                                                 <  1:371505/141‑1 (MQ=255)

                              aCGCTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGCTGTTTTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACAt                                                                                                                >  3:215074/1‑141 (MQ=255)

                              aCGCTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACAt                                                                                                                >  2:543378/1‑141 (MQ=255)

                                 cTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAACATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATAcc                                                                                                             >  1:852911/1‑141 (MQ=255)

                                 cTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATAcc                                                                                                             <  2:1047753/141‑1 (MQ=255)

                                    aaTTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTg                                                                                                          <  3:317649/141‑1 (MQ=255)

                                    aaTTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTg                                                                                                          <  1:1013884/141‑1 (MQ=255)

                                      tttGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGaa                                                                                                        >  4:84804/1‑141 (MQ=255)

                                      tttGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGaa                                                                                                        >  1:733353/1‑141 (MQ=255)

                                      tttGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACACACCTTGaa                                                                                                        >  2:341560/1‑141 (MQ=255)

                                      tttGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAAATGGTATTCAGAATATTTATGTATACAACATACCTTGaa                                                                                                        >  2:534580/1‑141 (MQ=255)

                                       ttGCGTCTTAATCCAATCATTTTGATGATTGCTTTTGTAGGCGAGTACCGAACTAACAATAATACACAATAATATGATGATGAGATTTAAAAAAAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGaaa                                                                                                       <  2:479329/141‑1 (MQ=255)

                                         gCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAAt                                                                                                     <  1:16972/141‑1 (MQ=255)

                                         gCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAAt                                                                                                     >  1:774572/1‑141 (MQ=255)

                                         gCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTCAAAAt                                                                                                     >  2:17602/1‑141 (MQ=255)

                                             cTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAgat                                                                                                 >  2:727060/1‑141 (MQ=255)

                                             cTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTAAAAAAAAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAgat                                                                                                 <  2:1087403/141‑1 (MQ=255)

                                                      aTCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTAc                                                                                        <  1:949887/141‑1 (MQ=255)

                                                      aTCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTAc                                                                                        <  2:659531/141‑1 (MQ=255)

                                                      aTCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTAc                                                                                        >  1:810013/1‑141 (MQ=255)

                                                      aTCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTAc                                                                                        <  4:210848/141‑1 (MQ=255)

                                                         aTTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACaaa                                                                                     >  4:187642/1‑141 (MQ=255)

                                                           tttGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACaaaaa                                                                                   >  2:6873/1‑141 (MQ=255)

                                                           tttGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACaaaaa                                                                                   <  2:526986/141‑1 (MQ=255)

                                                            ttGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAAc                                                                                  <  3:331913/141‑1 (MQ=255)

                                                              gCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACtt                                                                                >  1:94763/1‑141 (MQ=255)

                                                              gCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACtt                                                                                >  2:417226/1‑141 (MQ=255)

                                                               cTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACttt                                                                               >  1:600522/1‑141 (MQ=255)

                                                               cTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACttt                                                                               <  1:1073291/141‑1 (MQ=255)

                                                               cTGATTGATTATGTAAGCGAGTACAGAACCAACAATAATACAAAATAATATGATGATAAAATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACttt                                                                               <  2:1013119/141‑1 (MQ=255)

                                                                tgattgatTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAAATGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTa                                                                              >  2:360951/1‑141 (MQ=255)

                                                                         ttGTATGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAGACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGaat                                                                     >  2:680311/1‑140 (MQ=255)

                                                                         ttGTATGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAGACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGaat                                                                     >  3:76605/1‑140 (MQ=255)

                                                                           gTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAg                                                                   >  1:979872/1‑141 (MQ=255)

                                                                           gTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATAGATGAAAAg                                                                   >  4:242970/1‑141 (MQ=255)

                                                                                cGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAGAAATg                                                              >  4:382374/1‑141 (MQ=255)

                                                                                 gAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAGAAATGc                                                             <  1:259728/141‑1 (MQ=255)

                                                                                    tACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAGAAATGCAGc                                                          <  4:215074/141‑1 (MQ=255)

                                                                                        gAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAGAAATGCAGCTAGc                                                      <  3:232698/141‑1 (MQ=255)

                                                                                         aaCCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAGAAATGCAGCTAGCa                                                     <  2:47209/141‑1 (MQ=255)

                                                                                         aaCCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAGAAATGCAGCTAGCa                                                     <  2:323615/141‑1 (MQ=255)

                                                                                           ccAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAGAAATGCAGCTAGCATg                                                   <  1:564296/141‑1 (MQ=255)

                                                                                             aacaaTAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAGAAATGCAGCTAGCATGCt                                                 <  1:375299/141‑1 (MQ=255)

                                                                                              acaaTAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAGAAATGCAGCTAGCATGCta                                                <  1:1003028/141‑1 (MQ=255)

                                                                                              acaaTAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAGAAATGCAGCTAGCATGCta                                                <  2:994231/141‑1 (MQ=255)

                                                                                                aataatACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAGAAATGCAGCTAGCATGCtata                                              >  1:8847/1‑141 (MQ=255)

                                                                                                  taataCACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAGAAATGCAGCTAGCATGCtatata                                            >  2:969865/1‑141 (MQ=255)

                                                                                                  taataCACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAGAAATGCAGCTAGCATGCtatata                                            >  1:46138/1‑141 (MQ=255)

                                                                                                     taCACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAGAAATGCAGCTAGCATGCTATATACTa                                         <  2:695882/141‑1 (MQ=255)

                                                                                                        acaATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAGAAATGCAGCTAGCATGCTATATACTAAGa                                      >  1:804620/1‑141 (MQ=255)

                                                                                                          aataatATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAGAAATGCAGCTAACATGCTATATACTAAGATg                                    <  3:88754/141‑1 (MQ=255)

                                                                                                              atatGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAGAAATGCAGCTAGCATGCTATATACTAAGATGAGCa                                <  1:1016235/141‑1 (MQ=255)

                                                                                                              atatGATGATGAGATTTAAAAATAAAAAAAATGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAGAAATGCAGCTAGCATGCTATATACTAAGATGAGCa                                >  1:925236/1‑141 (MQ=255)

                                                                                                               tatGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAGAAATGCAGCTAGCATGCTATATACTAAGATGAGCAt                               <  1:778807/141‑1 (MQ=255)

                                                                                                                atgatgatgAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAGAAATGCAGCTAGCATGCTATATACTAAGATGAGCAtt                              <  2:106298/141‑1 (MQ=255)

                                                                                                                 tgatgatgaGATTACAAAATAAAAAAAATGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAGAAATGCAGCTAGCATGCTATATACTAAGATGAGCATTg                             >  3:218969/1‑141 (MQ=255)

                                                                                                                   atgatgaGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAGAAATGCAGCTAGCATGCTATATACTAAGATGAGCATTGaa                           <  2:331156/141‑1 (MQ=255)

                                                                                                                       tgaGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAGAAATGCAGCTAGCATGCTATATACTAAGATGAGCATTGAACCaa                       <  2:228284/141‑1 (MQ=255)

                                                                                                                          gaTTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAGAAATGCAGCTAGCATGCTATATACTAAGATGAGCATTGAACCAATTg                    >  1:350478/1‑141 (MQ=255)

                                                                                                                           agttATAAATAAAATAACTGGTGTTGCGAATATTTTTTTATTTAACATACCTTGAAAATGGCTGATATTTACAAAAACTTTACCGATGAACAGAAATGCAGCTAGCAGGCTGTATACTAAGATGAGCATTGAACCAATTGt                   >  2:270487/3‑141 (MQ=255)

                                                                                                                           aTTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAGAAATGCAGCTAGCATGCTATATACTAAGATGAGCATTGAACCAATTGt                   <  2:774572/141‑1 (MQ=255)

                                                                                                                             ttAAAAATAAAAAAAATGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAGAAATGCAGCTAGCATGCTATATACTAAGATGAGCATTGAACCAATTGTTc                 <  1:293082/141‑1 (MQ=255)

                                                                                                                                aaaataaaaaAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAGAAATGCAGCTAGCATGCTATATACTAAGATGAGCATTGAACCAATTGTTCTaa              <  4:107182/141‑1 (MQ=255)

                                                                                                                                aaaataaaaaAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAGAAATGCAGCTAGCATGCTATATACTAAGATGAGCATTGAACCAATTGTTCTaa              <  2:478414/141‑1 (MQ=255)

                                                                                                                                aaaataaaaaAACTGGAAGTCCGAATATATGAGTATAAAACATACCTTGAAAATAGATAAAATAAAAAAAAACTTTATCGATGAACAGAAATGCAGCTAGCATGCTATATACTAAGATGAGCATTGAACCAATTGTTCTaa              <  2:104748/141‑1 (MQ=255)

                                                                                                                                    taaaaaAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAGAAATGCAGCTAGCATGCTATATACTAAGATGAGCATTGAACCAATTGTTCTAATGTa          <  1:97554/141‑1 (MQ=255)

                                                                                                                                    taaaaaAACTGGTATTCCGAATATTCGTGGATAAAACATGCCTGGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAGAAATGCAGCTAGCATGCTATATACTAAGATGAGCATTGAACCAATTGTTCTAATGTa          <  3:242970/141‑1 (MQ=255)

                                                                                                                                     aaaaaaaCTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAGAAATGCAGCTAGCATGCTATATACTAAGATGAGCATTGAACCAATTGTTCTAATGTAg         >  2:119196/1‑141 (MQ=255)

                                                                                                                                     aaaaaaaCTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAGAAATGCAGCTAGCATGCTATATACTAAGATGAGCATTGAACCAATTGTTCTAATGTAg         <  2:852911/141‑1 (MQ=255)

                                                                                                                                        aaaaCTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAGAAATGCAGCTAGCATGCTATATACTAAGATGAGCATTGAACCAATTGTTCTAATGTAGTgg      <  2:153632/141‑1 (MQ=255)

                                                                                                                                         aaaCTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAGAAATGCAGCTAGCATGCTATATACTAAGATGAGCATTGAACCAATTGTTCTAATGTAGTGGt     <  1:86138/141‑1 (MQ=255)

ATTAATGCCAACTGTTTCGCCTTCCATTGAACGCTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAGAAATGCAGCTAGCATGCTATATACTAAGATGAGCATTGAACCAATTGTTCTAATGTAGTGGTTCA  >  USA300TCH1516_ALE/2027665‑2027945

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`