Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 2,102,773 A→G 5.1% intergenic (‑233/+83) USA300HOU_RS10625 ← / ← USA300HOU_RS10630 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,102,7730AG 5.1% 409.1 / 6.9 134intergenic (‑233/+83)USA300HOU_RS10625/USA300HOU_RS10630hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (72/55);  new base G (5/2);  total (77/57)
Fisher's exact test for biased strand distribution p-value = 6.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

Only 100 of 141 total aligned reads displayed.

ACATATGCTTTAACCGTGGCAATACCTTGTGCTTCCGCAGTGACTTGACCATCACTATTGATTGATACAATATTCGTTTGATCTGTTGTGTATTTCAATAACTTACTTTGATTAGATGGCTCTACTACAACATTTAAATCATATGTGTTGCCAACTTTTAGTGTTTTAAAACTATCTGGTATATTAACCGACTTTATCTCAGTTTCCGATGAAGCCGGTTGCGTTACAAAGTTTCGCCGTTATCCTCTGTCACGTTTCCAGTATATTCTTCGCCTAAAATT  >  USA300TCH1516_ALE/2102633‑2102913
                                                                                                                                            |                                                                                                                                            
aCATATGCTTTAACCGTGGCAATACCTTGTGCTTCCGCAGTGACTTGACCATCACTATTGATTGATACAATATTCGTTTGATCTGTTGTGTATTTCAATAACTTACTTTGATTAGATGGCTCTACTACAACATTTAAATCa                                                                                                                                              >  1:79903/1‑141 (MQ=255)
aCATATGCTTTAACCGTGGCAATACCTTGTGCTTCCGCAGTGACTTGACCATCACTATTGATTGATACAATATTCGTTTGATCTGTTGTGTATTTCAATAACTTACTTTGATTAGATGGCTCTACTACAACATTTAAATCa                                                                                                                                              >  1:983022/1‑141 (MQ=255)
aCATATGCTTTAACCGTGGCAATACCTTGTGCGTCCGCAGTGACTTGACCATCACTATTGATTGATACAATATTCGTTTGATCTGTTGTGTATTTCAATAACTTACTTTGATTAGATGGCTCTACTACAACATTTAAATCa                                                                                                                                              >  2:323152/1‑141 (MQ=255)
       cTTTAACCGTGGCAATACCTTGTGCTTCCGCAGTGACTTGACCATCACTATTGATTGATACAATATTCGTTTGATCTGTTGTGTATTTCAATAACTTACTTTGATTAGATGGCTCTACTACAACATTTAAATCATATgtgt                                                                                                                                       >  3:409099/1‑141 (MQ=255)
       cTTTAACCGTGGCAATACCTTGTGCTTCCGCAGTGACTTGACCATCACTATTGATTGATACAATATTCGTTTGATCTGTTGTGTATTTCAATAACTTACTTTGATTAGATGGCTCTACTACAACATTTAAATCATATgtgt                                                                                                                                       >  4:344865/1‑141 (MQ=255)
       cTTTAACCGTGGCAATACCTTGTGCTTCCGCAGTGACTTGACCATCACTATTGATTGATACAATATTCGTTTGATCTGTTGTGTATTTCAATAACTTACTTTGATTAGATGGCTCTACTACAACATTTAAATCATATgtgt                                                                                                                                       >  2:172326/1‑141 (MQ=255)
       cTTTAACCGTGGCAATACCTTGTGCTTCCGCAGTGACTTGACCATCACTATTGATTGATACAATATTCGTTTGATCTGTTGTGTATTTCAATAACTTACTTTGATTAGATGGCTCTACTACAACATTTAAATCATATgtgt                                                                                                                                       >  1:380504/1‑141 (MQ=255)
          tAACCGTGGCAATACCTTGTGCTTCCGCAGTGACTTGACCATCACTATTGAGTGATATAATATTCGTTGGATCTGTTGTGTATTTCAATAACTTACTTTGATTAGATGGCTCTACTACAACATTTAAATCATATGTGTTGc                                                                                                                                    <  3:223189/141‑1 (MQ=255)
           aaCCGTGGCAATACCTTGTGCTTCCGCAGTGACTTGACCATCACTATTGATTGATACAATATTCGTTTGATCTGTTGTGTATTTCAATAACTTACTTTGATTAGATGGCTCTACTACAACATTTAAATCATATGTGTTGcc                                                                                                                                   >  2:970925/1‑141 (MQ=255)
            aCCGTGGCAATACCTTGTGCTTCCGCAGTGACTTGACCATCACTATTGATTGATACAATATTCGTTTGATCTGTTGTGTATTTCAATAACTTACTTTGATTAGATGGCTCTACTACAACATTTAAATCATATGTGTTGCCa                                                                                                                                  <  2:1072836/141‑1 (MQ=255)
             ccGTGGCAATACCTTGTGCTTCCGCAGTGACTTGACCATCACTATTGATTGATACAATATTCGTTTGATCTGTTGTGTATTTCAATAACTTACTTTGATTAGATGGCTCTACTACAACATTTAAATCATATGTGTTGCCaa                                                                                                                                 >  1:615108/1‑141 (MQ=255)
             ccGTGGCAATACCTTGTGCTTCCGCAGTGACTTGACCATCACTATTGATTGATACAATATTCGTTTGATCTGTTGTGTATTTCAATAACTTACTTTGATTAGATGGCTCTACTACAACATTTAAATCATATGTGTTGCCaa                                                                                                                                 <  1:673361/141‑1 (MQ=255)
               gTGGCAATACCTTGTGCTTCCGCAGTGACTTGACCATCACTATTGATTGATACAATATTCGTTTGATCTGTTGTGTATTTCAATAACTTACTTTGATTAGATGGCTCTACTACAACATTTAAATCATATGTGTTGCCAACt                                                                                                                               <  1:504560/141‑1 (MQ=255)
                 ggCAATACCTTGTGCTTCCGCAGTGACTTGACCATCACTATTGATTGATACAATATTCGTTTGATCTGTTGTGTATTTCAATAACTTACTTTGATTAGATGGCTCTACTACAACATTTAAATCATATGTGTTGCCAACttt                                                                                                                             >  2:577470/1‑141 (MQ=255)
                 ggCAATACCTTGTGCTTCCGCAGTGACTTGACCATCACTATTGATTGATACAATATTCGTTTGATCTGTTGTGTATTTCAATAACTTACTTTGATTAGATGGCTCTACTACAACATTTAAATCATATGTGTTGCCAACttt                                                                                                                             >  2:538114/1‑141 (MQ=255)
                 ggCAATACCTTGTGCTTCCGCAGTGACTTGACCATCACTATTGATTGATACAATATTCGTTTGATCTGTTGTGTATTTCAATAACTTACTTTGATTAGATGGCTCTACTACAACATTTAAATCATATGTGTTGCCAACttt                                                                                                                             >  4:217819/1‑141 (MQ=255)
                   cAATACCTTGTGCTTCCGCAGTGACTTGACCATCACTATTGATTGATACAATATTCGTTTGATCTGTTGTGTATTTCAATAACTTACTTTGATTAGATGGCTCTACTACAACATTTAAATCATATGTGTTGCCAACTTTTa                                                                                                                           <  2:359848/141‑1 (MQ=255)
                    aaTACCTTGTGCTTCCGCAGTGACTTGACCATCACTATTGATTGATACAATATTCGTTTGATCTGTTGTGTATTTCAATAACTTACTTTGATTAGATGGCTCTACTACAACATTTAAATCATATGTGTTGCCAACTTTTAg                                                                                                                          <  1:978395/141‑1 (MQ=255)
                    aaTACCTTGTGCTTCCGCAGTGACTTGACCATCACTATTGATTGATACAATATTCGTTTGATCTGTTGTGTATTTCAATAACTTACTTTGATTAGATGGCTCTACTACAACATTTAAATCATATGTGTTGCCAACTTTTAg                                                                                                                          <  2:800958/141‑1 (MQ=255)
                     aTACCTTGTGCTTCCGCAGTGACTTGACCATCACTATTGATTGATACAATATTCGTTTGATCTGTTGTGTATTTCAATAACTTACTTTGATTAGATGGCTCTACTACAACATTTAAATCATATGTGTTGCCAACTTTTAgt                                                                                                                         <  1:295300/141‑1 (MQ=255)
                        ccTTGTGCTTCCGCAGTGACTTGACCATCACTATTGATTGATACAATATTCGTTTGATCTGTTGTGTATTTCAATAACTTACTTTGATTAGATGGCTCTACTACAACATTTAAATCATATGTGTTGCCAACTTTTAGTGtt                                                                                                                      >  2:107673/1‑141 (MQ=255)
                        ccTTGTGCTTCCGCAGTGACTTGACCATCACTATTGATTGATACAATATTCGTTTGATCTGTTGTGTATTTCAATAACTTACTTTGATTAGATGGCTCTACTACAACATTTAAATCATATGTGTTGCCAACTTTTAGTGtt                                                                                                                      >  2:927384/1‑141 (MQ=255)
                          ttGTGCTTCCGCAGTGACTTGACCATCACTATTGATTGATACAATATTCGTTTGATCTGTTGTGTATTTCAATAACTTACTTTGATTAGATGGCTCTACTACAACATTTAAATCATATGTGTTGCCAACTTTTAGTGtttt                                                                                                                    >  1:178382/1‑141 (MQ=255)
                            gtgCTTCCGCAGTGACTTGACCATCACTATTGATTGATACAATATTCGTTTGATCTGTTGTGTATTTCAATAACTTACTTTGATTAGATGGCTCTACTACAACATTTAAATCGTATGTGTTGCCAACTTTTAGTGTTTTaa                                                                                                                  >  3:403949/1‑141 (MQ=255)
                                 tCCGCAGTGACTTGACCATCACTATTGATTGATACAATATTCGTTTGATCTGTTGTGTATTTCAATAACTTACTTTGATTAGATGGCTCTACTACAACATTTAAATCATATGTGTTGCCAACTTTTAGTGTTTTAAAACTa                                                                                                             >  1:569136/1‑141 (MQ=255)
                                  ccGCAGTGACTTGACCATCACTATTGATTGATACAATATTCGTTTGATCTGTTGTGTATTTCAATAACTTACTTTGATTAGATGGCTCTACTACAACATTTAAATCATATGTGTTGCCAACTTTTAGTGTTTTAAAACTAt                                                                                                            >  3:64652/1‑141 (MQ=255)
                                  ccGCAGTGACTTGACCATCACTATTGATTGATACAATATTCGTTTGATCTGTTGTGTATTTCAATAACTTACTTTGATTAGATGGCTCTACTACAACATTTAAATCATATGTGTTGCCAACTTTTAGTGTTTTAAAACTAt                                                                                                            <  2:817717/141‑1 (MQ=255)
                                  ccGCAGTGACTTGACCATCACTATTGATTGATACAATATTCGTTTGATCTGTTGTGTATTTCAATAACTTACTTTGATTAGATGGCTCTACTACAACATTTAAATCATATGTGTTGCCAACTTTTAGTGTTTTAAAACTAt                                                                                                            >  2:435990/1‑141 (MQ=255)
                                      aGTGACTTGACCATCACTATTGATTGATACAATATTCGTTTGATCTGTTGTGTATTTCAATAACTTACTTTGATTAGATGGCTCTACTACAACATTTAAATCATATGTGTTGCCAACTTTTAGTGTTTTAAAATTATCTgg                                                                                                        <  1:861453/141‑1 (MQ=255)
                                         taCTTGACCATCTCTATTGATTGATACATTATTCGTTTGATCTGTTGTGTATTTCAATAACTTACTTTGATTAGATGGCTCTACTACAACATTTAAATCATATGTGTTGCCAACTTTTAGTGTTTTAAAACTATCTGGtat                                                                                                     <  2:1068498/140‑1 (MQ=255)
                                         gACTTGACCATCACTATTGATTGATACAATATTCGTTTGATCTGTTGTGTATTTCAATAACTTACTTTGATTAGATGGCTCTACTACAACATTTAAATCATATGTGTTGCCAACTTTTAGTGTTTTAAAACTATCTGGtgt                                                                                                     >  1:227458/1‑139 (MQ=255)
                                         gACTTGACCATCACTATTGATTGATACAATATTCGTTTGATCTGTTGTGTATTTCAATAACTTACTTTGATTAGATGGCTCTACTACAACATTTAAATCATATGTGTTGCCAACTTTTAGTGTTTTAAAACTATCTGGtat                                                                                                     >  1:640056/1‑141 (MQ=255)
                                         gACTTGACCATCACTATTGATTGATACAATATTCGAATGATCTGATATGAATACCAATAACTTACTTTGATTAAATGGCTATCCTACAACATTTAAATCATATGTGTTGCCAACTTTTCGTGTTTTAAAAGTATCtcgcct                                                                                                     >  4:339682/1‑136 (MQ=255)
                                          aCTTGACCATCACTATTGATTGATACAATATTCGTTTGATCTGTTGTGTATTTCAATAACTTACTTTGATTAGATGGCTCTACTACAACATTTAAATCATATGTGTTGCCAACTTTTAGTGTTTTAAAACTATCTGGtata                                                                                                    <  1:577726/141‑1 (MQ=255)
                                                ccATCACTATTGATTGATACAATATTCGTTTGATCTGTTGTGTATTTCAATAACTTACTTTGATTAGATGGCTCTACTACAACATTTAAATCATATGTGTTGCCAACTTTTAGTGTTTTAAAACTATCTGGTATATTAAcc                                                                                              <  3:87037/141‑1 (MQ=255)
                                                 cATCACTATTGATTGATACAATATTCGTTTGATCTGTTGTGTATTTCAATAACTTACTTTGATTAGATGGCTCTACTACAACATTTAAATCATATGTGTTGCCAACTTTTAGTGTTTTAAAACTATCTGGTATATTAACCg                                                                                             <  1:447332/141‑1 (MQ=255)
                                                   tCACTATTGATTGATACAATATTCGTTTGATCTGTTGTGTATTTCAATAACTTACTTTGATTAGATGGCTCTACTACAACATTTAAATCATATGTGTTGCCAACTTTTAGTGTTTTAAAACTATCTGGTATATTAACCGAc                                                                                           <  1:398026/141‑1 (MQ=255)
                                                   tCACTATTGATTGATACAATATTCGTTTGATCTGTTGTGTATTTCAATAACTTACTTTGATTAGATGGCTCTACTACAACATTTAAATCATATGTGTTGCCAACTTTTAGTGTTTTAAAACTATCTGGTATATTAACCGAc                                                                                           <  1:329366/141‑1 (MQ=255)
                                                   tCACTATTGATTGATACAATATTCGTTTGATCTGTTGTGTATTTCAATAACTTACTTTGATTAGATGGCTCTACTACAACATTTAAATCATATGTGTTGCCAACTTTTAGTGTTTTAAAACTATCTGGTATATTAACCGAc                                                                                           <  2:874716/141‑1 (MQ=255)
                                                   tCACTATTGATTGATACAATATTCGTTTGATCTGTTGTGTATTTCAATAACTTACTTTGATTAGATGGCTCTACTACAACATTTAAATCATATGTGTTGCCAACTTTTAGTGTTTTAAAACTATCTGGTATATTAACCGAc                                                                                           <  3:84078/141‑1 (MQ=255)
                                                     aCTATTGATTGATACAATATTCGTTTGATCTGTTGTGTATTTCAATAACTTACTTTGATTAGATGGCTCTACTACAACATTTAAATCATATGTGTTGCCAACTTTTAGTGTTTTAAAACTATCTGGTATATTAACCGACtt                                                                                         >  3:391411/1‑141 (MQ=255)
                                                          tgattgatACAATATTCGTTTGATCTGTTGTGTATTTCAATAACTTACTTTGATTAGATGGCTCTACTACAACATTTAAATCGTATGTGTCGCCAACTTTAAGTGTTTTAATGCTATCTGGTATATTAACCGACTTTaccg                                                                                    >  1:418832/1‑138 (MQ=255)
                                                           gattgatACAATATTCGTTTGATCTGTTGTGTATTTCAATAACTTACTTTGATTAGATGGCTCTACTACAACATTTAAATCATATGTGTTGCCAACTTTTAGTGTTTTAAAACTATCTGGTATATTAACCGACTTTAtctc                                                                                   >  1:903251/1‑141 (MQ=255)
                                                           gattgatACAATATTCGTTTGATCTGTTGTGTATTTCAATAACTTACTTTGATTAGATGGCTCTACTACAACATTTAAATCATATGTGTTGCCAACTTTTAGTGTTTTAAAACTATCTGGTATATTAACCGACTTTAtctc                                                                                   >  1:776449/1‑141 (MQ=255)
                                                           gattgatACAATATTCGTTTGATCTGTTGTGTATTTCAATAACTTACTTTGATTAGATGGCTCTACTACAACATTTAAATCATATGTGTTGCCAACTTTTAGTGTTTTAAAACTATCTGGTATATTAACCGACTTTAtctc                                                                                   >  3:390841/1‑141 (MQ=255)
                                                            attgatACAATATTCGTTTGATCTGTTGTGTATTTCAATAACTTACTTTGATTAGATGGCTCTACTACAACATTTAAATCATATGTGTTGCCAACTTTTAGTGTTTTAAAACTATCTGGTATATTAACCGACTTTATCTCa                                                                                  <  1:951986/141‑1 (MQ=255)
                                                                atACAATATTCGTTTGATCTGTTGTGTATTTCAATAACTTACTTTGATTAGATGGCTCTACTACAACATTTAAATCATATGTGTTGCCAACTTTTAGTGTTTTAAAACTATCTGGTATATTAACCGACTTTATCTCAGttt                                                                              >  1:1021848/1‑141 (MQ=255)
                                                                atACAATATTCGTTTGATCTGTTGTGTATTTCAATAACTTACTTTGATTAGATGGCTCTACTACAACATTTAAATCATATGTGTTGCCAACTTTTAGTGTTTTAAAACTATCTGGTATATTAACCGACTTTATCTCAGttt                                                                              >  1:273866/1‑141 (MQ=255)
                                                                atACAATATTCGTTTGATCTGTTGTGTATTTCAATAACTTACTTTGATTAGATGGCTCTACTACAACATTTAAATCATATGTGTTGCCAACTTTTAGTGTTTTAAAACTATCTGGTATATTAACCGACTTTATCTCAGttt                                                                              >  1:430357/1‑141 (MQ=255)
                                                                    aaTATTCGTTTGATCTGTTGTGTATTTCAATAACTTACTTTGATTAGATGGCTCTACTACAACATTTAAATCATATGTGTTGCCAACTTTTAGTGTTTTAAAACTATCTGGTATATTAACCGACTTTATCTCAGTTTCCGa                                                                          >  2:598104/1‑141 (MQ=255)
                                                                     atatTCGTTTGATCTGTTGTGTATTTCAATAACTTACTTTGATTAGATGGCTCTACTACAACATTTAAATCATATGTGTTGCCAACTTTTAGTGTTTTAAAACTATCTGGTATATTAACCGACTTTATCTCAGTTTCCGAt                                                                         <  2:406304/141‑1 (MQ=255)
                                                                     atatTCGTTTGATCTGTTGTGTATTTCAATAACTTACTTTGATTAGATGGCTCTACTACAACATTTAAATCATATGTGTTGCCAACTTTTAGTGTTTTAAAACTATCTGGTATATTAACCGACTTTATCTCAGTTTCCGAt                                                                         >  2:196294/1‑141 (MQ=255)
                                                                     atatTCGTTTGATCTGTTGTGTATTTCAATAACTTACTTTGATTAGATGGCTCTACTACAACATTTAAATCATATGTGTTGCCAACTTTTAGTGTTTTAAAACTATCTGGTATATTAACCGACTTTATCTCAGTTTCCGAt                                                                         >  1:361853/1‑141 (MQ=255)
                                                                      tatTCGTTTGATCTGTTGTGTATTTCAATAACTTACTTTGATTAGATGGCTCTACTACAAAATTTAAATCATATGTGTTGCCAACTTTTAGTGTTTTAAAACTATCTGGTATATTAACCGACTTTATCTCAGTTTCCGATg                                                                        <  1:44392/141‑1 (MQ=255)
                                                                        ttCGTTTGATCTGTTGTGTATTTCAATAACTTACTTTGATTAGATGGCTCTACTACAACATTTAAATCATATGTGTTGCCAACTTTTAGTGTTTTAAAACTATCTGGTATATTAACCGACTTTATCTCAGTTTCCGATGaa                                                                      >  2:823892/1‑141 (MQ=255)
                                                                        ttCGTTTGATCTGTTGTGTATTTCAATAACTTACTTTGATTAGATGGCTCTACTACAACATTTAAATCATATGTGTTGCCAACTTTTAGTGTTTTAAAACTATCTGGTATATTAACCGACTTTATCTCAGTTTCCGATGaa                                                                      <  4:235043/141‑1 (MQ=255)
                                                                        ttCGTTTGATCTGTTGTGTATTTCAATAACTTACTTTGATTAGATGGCTCTACTACAACATTTAAATCATATGTGTTGCCAACTTTTAGTGTTTTAAAACTATCTGGTATATTAACCGACTTTATCTCAGTTTCCGATGaa                                                                      >  4:330934/1‑141 (MQ=255)
                                                                          cGTTTGATCTGTTGTGTATTTCAATAACTTACTTTGATTAGATGGCTCTACTACAACATTTAAATCATATGTGTTGCCAACTTTTAGTGTTTTAAAACTATCTGGTATATTAACCGACTTTATCTCAGTTTCCGATGAAGc                                                                    >  2:732807/1‑141 (MQ=255)
                                                                            tttGATCTGTTGTGTATTTCAATAACTTACTTTGATTAGATGGCTCTACTACAACATTTAAATCATATGTGTTGCCAACTTTTAGTGTTTTAAAACTATCTGGTATATTAACCGACTTTATCTCAGTTTCCGATGAAGCCg                                                                  <  4:358940/141‑1 (MQ=255)
                                                                                 tCTGTTGTGTATTTCAATAACTTACTTTGATTAGATGGCTCTACTACAACATTTAAATCATATGTGTTGCCAACTTTTAGTGTTTTAAAACTATCTGGTATATTAACCGACTTTATCTCAGTTTCCGATGAAGCCGGTTGc                                                             >  3:122022/1‑141 (MQ=255)
                                                                                     ttgtGTATTTCAATTACTTACTTTGATTAGATGGCTCTACTACAACATTTAAATCGTATGTGTCGCCAACTTTAAGTGTTTTAATGCTATCTGGTATATTAACCGACTTTACCGCAGTTTCCGATGAAGCCGGttttgtta                                                         <  4:374599/141‑7 (MQ=255)
                                                                                     ttgtGTATTTCAATAACTTACTTTGATTAGATGGCTCTACTACAACATTTAAATCATATGTGTTGCCAACTTTTAGTGTTTTAAAACTATCTGGTATATTAACCGACTTTATCTCAGTTTCCGATGAAGCCGGTTGCGTTa                                                         >  1:354022/1‑141 (MQ=255)
                                                                                      tgtgTATTTCAATAACTTACTTTGATTAGATGGCTCTACTACAACATTTAAATCATATGTGTTGCCAACTTTTAGTGTTTTAAAACTATCTGGTATATTAACCGACTTTATCTCAGTTTCCGATGAAGCCGGTTGCGTTAc                                                        >  4:196562/1‑141 (MQ=255)
                                                                                        tgtATTTCAATAACGTACTTTGATTAGATGGCTCTACTACAACATTTAAATCATATGTGTTGCCAACTTTTAGTGTTTTAAAACTATCTGGTATATTAACCGACTTTAGCTCAGTTTCCGATGAAGCCGGTTGCGTTACaa                                                      <  4:446909/141‑1 (MQ=255)
                                                                                          tATTTCAATAACTTACTTTGATTAGATGGCTCTACTACAACATTTAAATCATATGTGTTGCCAACTTTTAGTGTTTTAAAACTATCTGGTATATTAACCGACNTNATCTCANTTTCCGATGAAGCCGGTTGCGTTACAAAg                                                    <  3:183554/141‑1 (MQ=255)
                                                                                              tCAATAACTTACTTTGATTAGATGGCTCTACTACAACATTTAAATCATATGTGTTGCCAACTTTTAGTGTTTTAAAACTATCTGGTATATTAACCGACTTTATCTCAGTTTCCGATGAAGCCGGTTGCGTTACAAAGTTTc                                                >  2:968478/1‑141 (MQ=255)
                                                                                                 aTAACTTACTTTGATTAGATGGCTCTACTACAACATTTAAATCATATGTGTTGCCAACTTTTAGTGTTTTAAAACTATCTGGTATATTAACCGACTTTATCTCAGTTTCCGATGAAGCCGGTTGCGTTACAAAGTTTCGcc                                             <  1:215617/141‑1 (MQ=255)
                                                                                                  tAACTTACTTTGATTAGATGGCTCTACTACAACATTTAAATCATATGTGTTGCCAACTTTTAGTGTTTTAAAACTATCTGGTATATTAACCGACTTTATCTCAGTTTCCGATGAAGCCGGTTGCGTTACAAAGTTTCGCCg                                            >  1:802671/1‑141 (MQ=255)
                                                                                                   aaCTTACTTTGATTAGATGGCTCTACTACAACATTTAAATCATATGTGTTGCCAACTTTTAGTGTTTTAAAACTATCTGGTATATTAACCGACTTTATCTCAGTTTCCGATGAAGCCGGTTGCGTTACAAAGTTTCGCCGt                                           >  1:442330/1‑141 (MQ=255)
                                                                                                    acttacttTGATTAGATGGCTCTACTACAACATTTAAATCATATGTGTTGCCAACTTTTAGTGTTTTAAAACTATCTGGTATATTAACCGACTTTATCTCAGTTTCCGATGAAGCCGGTTGCGTTACAAAGTTTCGCCGtt                                          <  3:69959/141‑1 (MQ=255)
                                                                                                     cttacttTGATTAGATGGCTCTACTACAACATTTAAATCATATGTGTTGCCAACTTTTAGTGTTTTAAAACTATCTGGTATATTAACCGACTTTATCTCAGTTTCCGATGAAGCCGGTTGCGTTACAAAGTTTCGCCGTTa                                         >  1:6010/1‑141 (MQ=255)
                                                                                                     cttacttTGATTAGATGGCTCTACTACAACATTTAAATCATATGTGTTGCCAACTTTTAGTGTTTTAAAACTATCTGGTATATTAACCGACTTTATCTCAGTTTCCGATGAAGCCGGTTGCGTTACAAAGTTTCGCCGTTa                                         >  1:369168/1‑141 (MQ=255)
                                                                                                     cttacttTGATTAGATGGCTCTACTACAACATTTAAATCATATGTGTTGCCAACTTTTAGTGTTTTAAAACTATCTGGTATATTAACCGACTTTATCTCAGTTTCCGATGAAGCCGGTTGCGTTACAAAGTTTCGCCGTTa                                         <  3:332167/141‑1 (MQ=255)
                                                                                                           ttGATTAGATGGCTCTACTACAACATTTAAATCATATGTGTTGCCAACTTTTAGTGTTTTAAAACTATCTGGTATATTAACCGACTTTATCTCAGTTTCCGATGAAGCCGGTTGCGTTACAAAGTTTCGCCGTTATCctct                                   <  2:160899/141‑1 (MQ=255)
                                                                                                              aTTAGATGGCTCTACTACAACATTTAAATCATATGTGTTGCCAACTTTTAGTGTTTTAAAACTATCTGGTATATTAACCGACTTTATCTCAGTTTCCGATGAAGCCGGTTGCGTTACAAAGTTTCGCCGTTATCCTCTGTc                                <  4:142310/141‑1 (MQ=255)
                                                                                                              aTTAGATGGCTCTACTACAACATTTAAATCATATGTGTTGCCAACTTTTAGTGTTTTAAAACTATCTGGTATATTAACCGACTTTATCTCAGTTTCCGATGAAGCCGGTTGCGTTACAAAGTTTCGCCGTTATCCTCTGTc                                <  2:380504/141‑1 (MQ=255)
                                                                                                              aTTAGATGGCTCTACTACAACATTTAAATCATATGTGTTGCCAACTTTTAGTGTTTTAAAACTATCTGGTATATTAACCGACTTTATCTCAGTTTCCGATGAAGCCGGTTGCGTTACAAAGTTTCGCCGTTATCCTCTGTc                                <  2:203550/141‑1 (MQ=255)
                                                                                                                   aTGGCTCTACTACAACATTTAAATCATATGTGTTGCCAACTTTTAGTGTTTTAAAACTATCTGGTATATTAACCGACTTTATCTCAGTTTCCGATGAAGCCGGTTTCGTTACAAAGTTTCGCCGTTATCCTCTGTCACGtt                           >  2:17349/1‑141 (MQ=255)
                                                                                                                   aTGGCTCTACTACAACATTTAAATCATATGTGTTGCCAACTTTTAGTGTTTTAAAACTATCTGGTATATTAACCGACTTTATCTCAGTTTCCGATGAAGCCGGTTGCGTTACAAAGTTTCGCCGTTATCCTCTGTCACGtt                           >  3:100857/1‑141 (MQ=255)
                                                                                                                   aTGGCTCTACTACAACATTTAAATCATATGTGTTGCCAACTTTTAGTGTTTTAAAACTATCTGGTATATTAACCGACTTTATCTCAGTTTCCGATGAAGCCGGTTGCGTTACAAAGTTTCGCCGTTATCCTCTGTCACGtt                           <  1:673959/141‑1 (MQ=255)
                                                                                                                     ggCTCTACTACAACATTTAAATCATATGTGTTGCCAACTTTTAGTGTTTTAAAACTATCTGGTATATTAACCGACTTTATCTCAGTTTCCGATGAAGCCGGTTGCGTTACAAAGTTTCGCCGTTATCCTCTGTCACGTTTc                         >  2:540561/1‑141 (MQ=255)
                                                                                                                      gCTCTACTACAACATTTAAATCATATGTGTTGCCAACTTTTAGTGTTTTAAAACTATCTGGTATATTAACCGACTTTATCTCAGTTTCCGATGAAGCCGGTTGCGTTACAAAGTTTCGCCGTTATCCTCTGTCACGTTTcc                        <  4:314826/141‑1 (MQ=255)
                                                                                                                      gCTCTACTACAACATTTAAATCATATGTGTTGCCAACTTTTAGTGTTTTAAAACTATCTGGTATATTAACCGACTTTATCTCAGTTTCCGATGAAGCCGGTTGCGTTACAAAGTTTCGCCGTTATCCTCTGTCACGTTTcc                        <  2:536531/141‑1 (MQ=255)
                                                                                                                       ctctACTACAACATTTAAATCATATGTGTTGCCAACTTTTAGTGTTTTAAAACTATCTGGTATATTAACCGACTTTATCTCAGTTTCCGATGAAGCCGGTTGCGTTACAAAGTTTCGCCGTTATCCTCTGTCACGTTTCCa                       >  2:497650/1‑141 (MQ=255)
                                                                                                                       ctctACTACAACATTTAAATCATATGTGTTGCCAACTTTTAGTGTTTTAAAACTATCTGGTATATTAACCGACTTTATCTCAGTTTCCGATGAAGCCGGTTGCGTTACAAAGTTTCGCCGTTATCCTCTGTCACGTTTCCa                       >  3:215600/1‑141 (MQ=255)
                                                                                                                           acaataacaTTTAAATCATATGTGTTGCCAACTTTTAGTGTTTTAAAACTATCTGGTATATTAACCGACTTTATCTCAGTTTCCGATGAAGCCGGTTGCGTTACAAAGTTTCGCCGTTATCCTCTGTCACGTTTCCAGtat                   >  4:216551/6‑141 (MQ=255)
                                                                                                                            ctacAACATTTAAATCATATGTGTTGCCAACTTTTAGTGTTTTAAAACTATCTGGTATATTAACCGACTTTATCTCAGTTTCCGATGAAGCCGGTTGCGTTACAAAGTTTCGCCGTTATCCTCTGTCACGTTTCCAGtata                  >  3:47036/1‑141 (MQ=255)
                                                                                                                                     ttAAATCATATGTGTTGCCAACTTTTAGTGTTTTAAAACTATCTGGTATATTAACCGACTTTATCTCAGTTTCCGATGAAGCCGGTTGCGTTACAAAGTTTCGCCGTTATCCTCTGTCACGTTTCCAGTATATTCTTCGcc         <  2:174132/141‑1 (MQ=255)
                                                                                                                                      tAAATCATATGTGTTGCCAACTTTTAGTGTTTTAAAACTATCTGGTATATTAACCGACTTTATCTCAGTTTCCGATGAAGCCGGTTGCGTTACAAAGTTTCGCCGTTATCCTCTGTCACGTTTCCAGTATATTCTTCGCCt        >  2:222294/1‑141 (MQ=255)
                                                                                                                                      tAAATCATATGTGTTGCCAACTTTTAGTGTTTTAAAACTATCTGGTATATTAACCGACTTTATCTCAGTTTCCGATGAAGCCGGTTGCGTTACAAAGTTTCGCCGTTATCCTCTGTCACGTTTCCAGTATATTCTTCGCCt        >  2:974955/1‑141 (MQ=255)
                                                                                                                                      tAAATCATATGTGTTGCCAACTTTTAGTGTTTTAAAACTATCTGGTATATTAACCGACTTTATCTCAGTTTCCGATGAAGCCGGTTGCGTTACAAAGTTTCGCCGTTATCCTCTGTCACGTTTCCAGTATATTCTTCGCCt        >  1:608137/1‑141 (MQ=255)
                                                                                                                                        aaTCATATGTGTTGCCAACTTTTAGTGTTTTAAAACTATCTGGTATATTAACCGACTTTATCTCAGTTTCCGATGAAGCCGGTTGCGTTACAAAGTTTCGCCGTTATCCTCTGTCACGTTTCCAGTATATTCTTCGCCTaa      >  1:662486/1‑141 (MQ=255)
                                                                                                                                        aaTCATATGTGTTGCCAACTTTTAGTGTTTTAAAACTATCTGGTATATTAACCGACTTTATCTCAGTTTCCGATGAAGCCGGTTGCGTTACAAAGTTTCGCCGTTATCCTCTGTCACGTTTCCAGTATATTCTTCGCCTaa      >  2:734620/1‑141 (MQ=255)
                                                                                                                                         aTCATATGTGTTGCCAACTTTTAGTGTTTTAAAACTATCTGGTATATTAACCGACTTTATCTCAGTTTCCGATGAAGCCGGTTGCGTTACAAAGTTTCGCCGTTATTCTCTGTCACGTTTCCAGTATATTCTTCGCCTaaa     >  2:632921/1‑141 (MQ=255)
                                                                                                                                         aTCATATGTGTTGCCAACTTTTAGTGTTTTAAAACTATCTGGTATATTAACCGACTTTATCTCAGTTTCCGATGAAGCCGGTTGCGTTACAAAGTTTCGCCGTTATCCTCTGTCACGTTTCCAGTATATTCTTCGCCTaaa     <  3:344863/141‑1 (MQ=255)
                                                                                                                                          tCATATGTGTTGCCAACTTTTAGTGTTTTAAAACTATCTGGTATATTAACCGACTTTATCTCAGTTTCCGATGAAGCCGGTTGCGTTACAAAGTTTCGCCGTTATCCTCTGTCACGTTTCCAGTATATTCTTCGCCTaaaa    <  1:527068/141‑1 (MQ=255)
                                                                                                                                           cATATGTGTTGCCAACTTTTAGTGTTTTAAAACTATCTGGTATATTAACCGACTTTATCTCAGTTTCCGATGAAGCCGGTTGCGTTACAAAGTTTCGCCGTTATCCTCTGTCACGTTTCCAGTATATTCTTCGCCTAAAAt   <  4:262033/141‑1 (MQ=255)
                                                                                                                                           cATATGTGTTGCCAACTTTTAGTGTTTTAAAACTATCTGGTATATTAACCGACTTTATCTCAGTTTCCGATGAAGCCGGTTGCGTTACAAAGTTTCGCCGTTATCCTCTGTCACGTTTCCAGTATATTCTTCGCCTAAAAt   <  1:173387/141‑1 (MQ=255)
                                                                                                                                            atatGTGTTGCCAACTTTTAGTGTTTTAAAACTATCTGGTATATTAACCGACTTTATCTCAGTTTCCGATGAAGCCGGTTGCGTTACAAAGTTTCGCCGTTATCCTCTGTCACGTTTCCAGTATATTCTTCGCCTAAAAtt  >  3:398261/1‑141 (MQ=255)
                                                                                                                                            atatGTGTTGCCAACTCTTAGTGTTTTAAAACTATCTGGTATATTAACCGCCTTTATCTCAGTTTCCGATGAAGCCGGTTGCGTTACAAAGTTTCGCCGTTATCCTCTGTCACGTTTCCAGTATATTCTTCGCCTAAAAtt  >  4:155956/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                            
ACATATGCTTTAACCGTGGCAATACCTTGTGCTTCCGCAGTGACTTGACCATCACTATTGATTGATACAATATTCGTTTGATCTGTTGTGTATTTCAATAACTTACTTTGATTAGATGGCTCTACTACAACATTTAAATCATATGTGTTGCCAACTTTTAGTGTTTTAAAACTATCTGGTATATTAACCGACTTTATCTCAGTTTCCGATGAAGCCGGTTGCGTTACAAAGTTTCGCCGTTATCCTCTGTCACGTTTCCAGTATATTCTTCGCCTAAAATT  >  USA300TCH1516_ALE/2102633‑2102913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: