BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	2,120,582	C→T	7.0%	V65I (GTT→ATT)	USA300HOU_RS10785 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,120,582	0	C	T	7.0%	381.7 / 10.2	123	V65I (GTT→ATT)	USA300HOU_RS10785	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (49/65); new base T (4/5); total (53/70)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 124 total aligned reads displayed.

AAGTATATTCGTCGAATAACGTCTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAA  >  USA300TCH1516_ALE/2120445‑2120722

aaGTATATTCGTCGAATAACGTCTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACt                                                                                                                                           <  2:928743/141‑1 (MQ=255)

aaGTATATTCGTCGAATAACGTCTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACt                                                                                                                                           <  2:554004/141‑1 (MQ=255)

aaGTATATTCGTCGAATAACGTCTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACt                                                                                                                                           <  1:789936/141‑1 (MQ=255)

  gTATATTCGTCGAATAACGTCTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACttt                                                                                                                                         >  2:846708/1‑141 (MQ=255)

   tataTTCGTCGAATAACGTCTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTg                                                                                                                                        <  2:32073/141‑1 (MQ=255)

    atatTCGTCGAATAACGTCTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGt                                                                                                                                       <  2:237073/141‑1 (MQ=255)

       ttCGTCGAATAACGTCTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTc                                                                                                                                    >  3:248214/1‑141 (MQ=255)

         cgtcgAATAACGTCTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCaa                                                                                                                                  >  4:72286/1‑141 (MQ=255)

          gtcgAATAACGTCTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCaaa                                                                                                                                 >  1:244324/1‑141 (MQ=255)

           tcgAATAACGTCTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCaaaa                                                                                                                                >  3:13100/1‑141 (MQ=255)

              aaTAACGTCTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAatat                                                                                                                             >  2:852966/1‑141 (MQ=255)

               aTAACGTCTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATc                                                                                                                            >  1:423209/1‑141 (MQ=255)

                 aaCGTCTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTa                                                                                                                          >  2:460266/1‑141 (MQ=255)

                  aCGTCTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTaa                                                                                                                         <  2:357028/141‑1 (MQ=255)

                   cGTCTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTaaa                                                                                                                        <  2:925397/141‑1 (MQ=255)

                     tCTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGc                                                                                                                      <  1:556403/141‑1 (MQ=255)

                     tCTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGc                                                                                                                      <  2:309058/141‑1 (MQ=255)

                      cTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCt                                                                                                                     >  2:690847/1‑141 (MQ=255)

                      cTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCt                                                                                                                     >  4:330671/1‑141 (MQ=255)

                        tAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCtgt                                                                                                                   <  1:363441/141‑1 (MQ=255)

                         aaaaaTATTCATTATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCGAAGTATCTAACGCtgtg                                                                                                                  <  4:23648/141‑1 (MQ=255)

                         aaaaaTATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACCCTTTGTTTCAAAATATCTAAAGCtgtg                                                                                                                  <  2:321221/141‑1 (MQ=255)

                         aaaaaTATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCtgtg                                                                                                                  >  1:401801/1‑141 (MQ=255)

                           aaaTATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGta                                                                                                                <  2:280028/141‑1 (MQ=255)

                            aaTATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGtat                                                                                                               <  1:62857/141‑1 (MQ=255)

                                ttCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGAt                                                                                                           >  2:807668/1‑141 (MQ=255)

                                ttCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGAt                                                                                                           <  1:531974/141‑1 (MQ=255)

                                ttCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGAt                                                                                                           >  2:590673/1‑141 (MQ=255)

                                ttCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGAt                                                                                                           <  1:982089/141‑1 (MQ=255)

                                ttCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTAATTAGTCTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAATACTTTGTTTCAAAATATCTAAAGCTGTGTATAGAt                                                                                                           <  2:84671/141‑1 (MQ=255)

                                 tCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATc                                                                                                          <  1:849402/141‑1 (MQ=255)

                                  cAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACATAGTTTCAAAATATCTAAAGCTGTGTATAGATCg                                                                                                         >  3:16317/1‑141 (MQ=255)

                                  cAATATATTTCTTACTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCACAACATCTAAAGCTGTGTATAGATCg                                                                                                         <  4:119769/141‑1 (MQ=255)

                                    atatatTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGtt                                                                                                       <  2:487681/141‑1 (MQ=255)

                                    atatatTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGtt                                                                                                       <  4:29834/141‑1 (MQ=255)

                                          ttctttttTTGTCTCTCTTGCTTTTTCCTTCCCTTTCATGACTAAGTTCATTAGTTTGTCCTGTTCTTCTTGGTTATTTGCAGTCCATTCATTGACCCTCTGTTTCCAAATATCTAAAGCTGTGTATAGCGCGTTCtcatc                                                                                                 <  4:242179/141‑1 (MQ=255)

                                          ttctttctGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCtcatc                                                                                                 <  2:455805/141‑1 (MQ=255)

                                           tctttctGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCtcatca                                                                                                <  2:945759/141‑1 (MQ=255)

                                            ctttctGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAg                                                                                               >  2:189671/1‑141 (MQ=255)

                                              ttctGTATCTCTCATGCTTATTCCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCTTAAACACTTTGTTGCAAAATATCTAAAGCTGTGTATAGAGCGTTCTCCTCAGaa                                                                                             <  4:321980/141‑1 (MQ=37)

                                              ttctGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGaa                                                                                             <  4:138289/141‑1 (MQ=255)

                                              ttctGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTCCTCATCAGaa                                                                                             <  2:423209/141‑1 (MQ=255)

                                                ttGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAAc                                                                                           <  2:983169/140‑1 (MQ=255)

                                                ctGTATCACTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAAc                                                                                           >  3:402607/1‑141 (MQ=255)

                                                   tATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAg                                                                                        >  2:436533/1‑141 (MQ=255)

                                                     tctctcATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTa                                                                                      <  3:1018/141‑1 (MQ=255)

                                                       tctcATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAAc                                                                                    >  2:700825/1‑141 (MQ=255)

                                                       tctcATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAAc                                                                                    <  1:989805/141‑1 (MQ=255)

                                                       tctcATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAAc                                                                                    <  1:61745/141‑1 (MQ=255)

                                                        ctcATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAAcc                                                                                   >  1:748148/1‑141 (MQ=255)

                                                          cATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCg                                                                                 >  2:160075/1‑141 (MQ=255)

                                                               ttATGCCTACCAGTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCGATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAAt                                                                            <  4:389994/141‑1 (MQ=255)

                                                               ttATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAAt                                                                            <  4:139155/141‑1 (MQ=255)

                                                                tataCCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAAtt                                                                           <  3:318603/141‑1 (MQ=255)

                                                                 ataCCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTg                                                                          >  1:522359/1‑141 (MQ=255)

                                                                  tacctaccATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGa                                                                         <  1:238647/141‑1 (MQ=255)

                                                                  tacctaccATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACCAGTAACCCGTCAATTGa                                                                         >  1:603800/1‑141 (MQ=255)

                                                                   acctaccATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCNCATCAGAAACTANTAACCCGTCAATTGaa                                                                        <  1:809794/141‑1 (MQ=255)

                                                                     ctaccATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAAtt                                                                      <  1:449886/141‑1 (MQ=255)

                                                                        ccATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTcc                                                                   <  4:370895/141‑1 (MQ=255)

                                                                          aTTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCtt                                                                 <  4:197905/141‑1 (MQ=255)

                                                                          aTTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCtt                                                                 <  2:1030522/141‑1 (MQ=255)

                                                                           tttCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTc                                                                <  2:890044/141‑1 (MQ=255)

                                                                              cATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATg                                                             >  2:723607/1‑141 (MQ=255)

                                                                              cATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATg                                                             <  1:886707/141‑1 (MQ=255)

                                                                               aTGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATgg                                                            >  2:587370/1‑141 (MQ=255)

                                                                                  aCTAAGTTAATTAGTCTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAATACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCGTCAGAAACTAGTAGCCCGTCAATTGAATTTCCTTCATGATCt                                                         <  2:1076496/141‑1 (MQ=38)

                                                                                         tCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCGTAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGaacaa                                                  <  1:852966/141‑1 (MQ=255)

                                                                                          cATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTTGaacaac                                                 <  3:402656/141‑1 (MQ=255)

                                                                                          cATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGaacaac                                                 >  1:714974/1‑141 (MQ=255)

                                                                                              aGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCGGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCAtt                                             <  1:281820/141‑1 (MQ=255)

                                                                                                   gTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGAc                                        <  3:286398/141‑1 (MQ=255)

                                                                                                   gTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGCTTCAAAATATCGAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTCAATTTCCTCCATGGACTCGAACAACCATTTCGat                                        >  4:430596/1‑140 (MQ=255)

                                                                                                    tCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACg                                       >  4:238133/1‑141 (MQ=255)

                                                                                                    tCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACg                                       >  2:1065026/1‑141 (MQ=255)

                                                                                                    tCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACg                                       >  3:273068/1‑141 (MQ=255)

                                                                                                     ccTGTTCATCTGTGTTATTTTCAATCCATTCATAAATACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCGTCAGAAACTAGTAGCCCGTCAATTGAATTTCCTTCATGATCTAAAACGACTATTTCGAcac                                      >  2:705909/1‑139 (MQ=255)

                                                                                                     ccTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGc                                      >  1:663286/1‑141 (MQ=255)

                                                                                                     ccTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGc                                      >  2:760113/1‑141 (MQ=255)

                                                                                                         ttCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCtata                                  >  2:923004/1‑141 (MQ=255)

                                                                                                         ttCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCtata                                  >  2:538008/1‑141 (MQ=255)

                                                                                                          tCATCTGTGTTATTTTCAATCCATTCATAAATACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCGTCAGAAACTAGTAGCCCGTCAATTGAATTTCCTTCATGATCTAAAACGACTATTTCGACACtatat                                 >  1:734959/1‑140 (MQ=255)

                                                                                                          tCATCTGTGTTATTTTCAATCCATTCATAAATACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCGTCAGAAACTAGTAGCCCGTCAATTGAATTTCCTTCATGATCTAAAACGACTATTTCGACACtatat                                 >  1:526314/1‑140 (MQ=255)

                                                                                                              cTGTGTTCTTTTCCATCCCTTCATCATTACTTTGTTCCAATCTATCTAAATCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTc                             <  3:199071/141‑1 (MQ=255)

                                                                                                                gtgtTATTTTCAATCCATTCATAAATACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCGTCAGAAACTAGTAGCCCGTCAATTGAATTTCCTTCATGATCTAAAACGACTATTTCGACACTATATGCTCgc                           <  1:455632/141‑2 (MQ=255)

                                                                                                                gtgtTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGt                           >  2:635553/1‑141 (MQ=255)

                                                                                                                     aTTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCtt                      <  1:299598/141‑1 (MQ=255)

                                                                                                                      ttttCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTa                     <  4:322472/141‑1 (MQ=255)

                                                                                                                          cAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAActc                 <  1:309741/141‑1 (MQ=255)

                                                                                                                          cAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAActc                 >  1:372840/1‑141 (MQ=255)

                                                                                                                           aaTCCATTCATACACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAActct                <  3:321195/141‑1 (MQ=255)

                                                                                                                            aTCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCtt               <  1:846708/141‑1 (MQ=255)

                                                                                                                             tCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTa              >  4:131355/1‑141 (MQ=255)

                                                                                                                             tCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTa              >  1:475000/1‑141 (MQ=255)

                                                                                                                             tCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTa              >  1:992887/1‑141 (MQ=255)

                                                                                                                                 ttCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTg          <  1:641314/141‑1 (MQ=255)

                                                                                                                                  tCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGa         <  3:281012/141‑1 (MQ=255)

                                                                                                                                     tAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGaaaa      >  2:1046637/1‑141 (MQ=255)

                                                                                                                                       aaCACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATc    <  2:730749/141‑1 (MQ=255)

                                                                                                                                         cacTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGGTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCaa  >  1:432525/1‑141 (MQ=255)

AAGTATATTCGTCGAATAACGTCTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTTCGACGCTATACGCTCGTTTCTTAACTCTTAATTGAAAATCAA  >  USA300TCH1516_ALE/2120445‑2120722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`