Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 2,146,840 A→T 5.1% intergenic (+228/+369) USA300HOU_RS10950 → / ← hld 2‑oxoglutaramate amidase/Delta‑hemolysin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,146,8400AT 5.1% 248.6 / 6.0 100intergenic (+228/+369)USA300HOU_RS10950/hld2‑oxoglutaramate amidase/Delta‑hemolysin
Reads supporting (aligned to +/- strand):  ref base A (46/47);  new base T (2/3);  total (50/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.25e-01

Only 100 of 101 total aligned reads displayed.

ATAGATAACTCAGTAAGAACCCATTTCGCCCTTTGCAAATGAATGATTTTCTTAATTAAATTGATTAATTTTTTGACCTTTTCCAACATTAGACTTATTCATATATTTTAACGGCGGGTCTCATAATGATGATTAACTCATCCCTTCTTCATTAC‑‑‑AA‑‑‑AAAAGGCCGCGAGCTTGGGAGGGGCTCACGACCATACTTATTATTAAGGGAATGTTTTACAGTTATTTTTTCAATCTATTTTTGGGGATGTTATTAATTATGAAAAAAATTTTATTTATC  >  USA300TCH1516_ALE/2146701‑2146977
                                                                                                                                           |                                                                                                                                               
ataGATAACTCAGTAAGAACCCATTTCGCCCTTTGCAAATGAATGATTTTCTTAATTAAATTGATTAATTTTTTGACCTTTTCCAACATTAGACTTATTCATATATTTTAACGGCGGGTCTCATAATGATGATTAACTCAt                                                                                                                                                <  2:1043845/141‑1 (MQ=255)
  aGATAACTCAGTAAGAACCCATTTCGCCCTTTGCAAATGAATGATTTTCTTAATTAAATTGATTAATTTTTTGACCTTTTCCAACATTAGACTTATTCATATATTTTAACGGCGGGTCTCATAATGATGGTTAAAtcctcc                                                                                                                                              >  2:529925/1‑141 (MQ=255)
   gATAACTCAGTAAGAACCCATTTCGCCCTTTGCAAATGAATGATTTTCTTAATTAAATTGATTAATTTTTTGACCTTTTTCAACATTAGACTTATTCATATATTTTAACGGCGGGTCTCATAATGATGATTAACTTATtcc                                                                                                                                             >  2:209932/1‑141 (MQ=255)
   gATAACTCAGTAAGAACCCATTTCGCCCTTTGCAAATGAATGATTTTCTTAATTAAATTGATTAATTTTTTGACCTTTTCCAACATTAGACTTATTCATATATTTTAACGGCGGGTCTCATAATGATGATTAACTCATccc                                                                                                                                             <  2:98380/141‑1 (MQ=255)
        cTCAGTAAGAACCCATTTCGCCCTTTGCAAATGAATGATTTTCTTAATTAAATTGATTAATTTTTTGACCTTTTCCAACATTAGACTTATTCATATATTTTAACGGCGGGTCTCATAATGATGATTAACTCATCCcttctt                                                                                                                                        >  1:708249/1‑141 (MQ=255)
         tCAGTAAGAACCCATTTCGCCCATTGTAAATGAATGATTTTCTTAATTAAATTGATTAATTTTTTGACCTTTTCCAACATTAGACTTATTCATATATTTTAACGGCGGGTCTCATAATGATGATTAACTCATCCCttcttc                                                                                                                                       <  2:709297/141‑1 (MQ=255)
          cAGTAAGAATCCATTTCGCCCTTTGCAAATGAATGATTTTCTTAATTAAATTGATTAATTTTTTGACCTTTTCCAACATTAGACTTATTCATATATTTTAACGGTGGGTCTCATAATGAGGATTAACTCTCCCcttcttta                                                                                                                                      >  3:76445/1‑139 (MQ=255)
          cAGTAAGAACCCATTTCGCCCTTTGCAAATGAATGATTTTCTTAATTAAATTGATTAATTTTTTGACCTTTTCCAACATTAGACTTATTCATATATTTTAACGGCGGGTCTCATAATGATGATTAACTCATCCCTTCTTCa                                                                                                                                      >  1:172351/1‑141 (MQ=255)
           aGTAAGAACCCATTTCGCCCTTTGCAAATGAATGATTTTCTTAATTAAATTGATTAATTTTTTGACCTTTTCCAACATTAGACTTATTCATATATTTTAACGGCGGGTCTCATAATGGTGATTAACTCATCCCTTTTTCAt                                                                                                                                     >  1:55907/1‑141 (MQ=255)
           aGTAAGAACCCATTTCGCCCTTTGCAAATGAATGATTTTCTTAATTAAATTGATTAATTTTTTGACCTTTTCCAACATTAGACTTATTCATATATTTTAACGGCGGGTCTCATAATGATGATTAACTCATCCCTTCTTCAt                                                                                                                                     >  3:335030/1‑141 (MQ=255)
           aGTAAGAACCCATTTCGCCCTTTGCAAATGAATGATTTTCTTAATTAAATTGATTAATTTTTTGACCTTTTCCAACATTAGACTTATTCATATATTTTAACGGCGGGTCTCATAATGATGATTAACTCATCCCTTCTTCAt                                                                                                                                     <  1:924190/141‑1 (MQ=255)
           aGTAAGAACCCATTTCGCCCTTTGCAAATGAATGATTTTCTTAATTAAATTGATTAATTTTTTGACCTTTTCCAACATTAGACTTATTCATATATTTTAACGGCGGGTCTCATAATGATGATTAACTCATCCCTTATACAt                                                                                                                                     <  4:136406/141‑1 (MQ=255)
           aGTAAGAACCCATTTCGCCCTGTGCAAATGAATGATTTTCTTAATTAAATTGATTAATTTTTTGACCTTTTCCAACATTAGACTTATTCATATATTTTAACGGCGGGTCTCATAATGATGATTAACTCATCCCTTCTTCAt                                                                                                                                     <  3:43623/141‑1 (MQ=255)
            gTAAGAACCCATTTCGCCCTTTGCAAATGAATGATTTTCTTAATTAAATTGATTAATTTTTTTTTTTTTTTTAACATTAGACTTATTCATATATTTTAACGGCGGGTCTCATAATGATGATTAACTCATTCCCTCTTCAtt                                                                                                                                    >  2:342120/1‑141 (MQ=255)
              aaGAACCCATTTCGCCCTTTGCAAATGAATGATTTTCTTAATTAAATTGATTAATTTTTTGACCTTTTCCAACATTAGACTTATTCATATATTTTAACGGCGGGTCTCATAATGATGATTAACTCATCCCTTCTTCATTAc                                                                                                                                  <  2:892011/141‑1 (MQ=255)
              aaGAACCCATTTCGCCCTTTGCAAATGAATGATTTTCTTAATTAAATTGATTAATTTTTTGACCTTTTCCAACATTAGACTTATTCATATATTTTAACGGCGGGTCTCATAATGATGATTAACTCATCCCTTCTTCATTAc                                                                                                                                  >  2:628717/1‑141 (MQ=255)
              aaGAACCCATTTCGCCCTTTGCAAATGAATGATTTTCTTAATTAAATTGATTAATTTTTTGACCTTTTCCAACATTAGACTTATTCATATATTTTAACGGCGGGTCTCATAATGATGATTAACTCATCCCCTCTTCATTAc                                                                                                                                  <  1:592641/141‑1 (MQ=255)
                 aaCCCATTTCGCCCTTTGCAAATGAATGATTTTCTTAATTAAATTGATTAATTTTTTGACCTTTTCCAACATTAGACTTATTCATATATTTTAACGGCATGTCTCAGAATGATGATTAACTCATCCCTTCTTCATTAC‑‑‑aa‑‑‑a                                                                                                                         <  3:331425/141‑1 (MQ=255)
                    ccATTTCGCCCTTTGCAAATGAATGATTTTCTTAATTAAATTGATTAATTTTTTGACCTTTTCCAACATTAGACTTATTCATATATTTTAACGGCGGGTCTCATAATGATGATTAACTCATCCCTTCTTCATTAC‑‑‑aa‑‑‑aaaa                                                                                                                      >  3:220664/1‑141 (MQ=255)
                          cGCCCTTTGCAAATGAATGATTTTCTTAATTAAATTGATTAATTTTTTGACCTTTTCCAACATTAGACTTATTCATATATTTTAACGGCGGGTCTCATAATGATGATTAACTCATCCCTTCTTCATTAC‑‑‑AA‑‑‑AAAAGGCcgc                                                                                                                <  4:346908/141‑1 (MQ=255)
                           gCCCTTTGCAAATGAATGATTTTCTTAATTAAATTGATTAATTTTTTGACCTTTTCCAACATTAGACTTATTCATATATTTTAACGGCGGGTCTCATAATGATGATTAACTCATCCCTTCTTCATTAC‑‑‑AA‑‑‑AAAAGGCcgcg                                                                                                               <  3:404215/141‑1 (MQ=255)
                           gCCCTTTGCAAATGAATGATTTTCTTAATTAAATTGATTAATTTTTTGACCTTTTCCAACATTAGACTTATTCATATATTTTAACGGCGGGGCTCATAATGATGATTAACTCATCCCTTCTTCATTAC‑‑‑AA‑‑‑AAAAGGCcgcg                                                                                                               >  3:348192/1‑141 (MQ=255)
                            cccTTTGCAAATGAATGATTTTCTTAATTAAATTGATTAATTTTTTGACCTTTTCCAACATTAGACTTATTCATATATTTTAACGGCGGGTCTCATAATGATGATTAACTCATCCCTTCTTCATTAC‑‑‑AA‑‑‑AAAAGGCCGCGa                                                                                                              >  3:32013/1‑141 (MQ=255)
                            cccTTTGCAAATGAATGATTTTCTTAATTAAATTGATTAATTTTTTGACCTTTTCCAACATTAGACTTATTCATATATTTTAACGGCGGGTCTCATAATGATGATTAACTCATCCCTTCTAAATTAC‑‑‑AA‑‑‑AAAAGGCCGCGa                                                                                                              <  2:166568/141‑1 (MQ=255)
                            cccTTTGCAAATGAATGATTTTCTTAATTAAATTGATTAATTTTTTGACCTTTTCCAACATTAGACTTATTCATATATTTTAACGGCGGGTATCATAATGATGTTTTAATCATTCCTTCTTGATTAC‑‑‑AC‑‑‑AAAAGGCcgcgc                                                                                                              >  2:396897/1‑140 (MQ=255)
                             ccTTTGCAAATGAATGATTTTCTTAATTAAATTGATTAATTTTTTGACATTTTCCAACATTAGACTTATGTATATATTTGAACGTCGGGTCTCAGAATGATGATTAACTCATCCCTTCTTCATTAC‑‑‑AA‑‑‑AAAAGGCCGCGAg                                                                                                             >  3:87634/1‑141 (MQ=255)
                              cTTTGCAAATGAATGATTTTCTTAATTAAATTGATTAATTTTTTGACCTTTTCCAACATTAGACTTATTCATATATTTTAACGGTGGGTCTCATAATGAGGATTAACTCTCCCCTTCTTTATTACAATAA‑‑‑AAAAGGCcgcg                                                                                                               >  4:108470/1‑141 (MQ=255)
                                   cAAATGAATGATTTTCTTAATTAAATTGATTACTTGTTTGACCTTTTCCAACATTAGACTTATTCATATATTTTAACGGCGGGTCTCATAATGATGATTAACTCATCCCTTCTTCATTAC‑‑‑AA‑‑‑AAAAGGCCGCGAGCTTggg                                                                                                       <  1:1052680/141‑1 (MQ=255)
                                    aaaTGAATGATTTTCTTAATTAAATTGATTAATTTTTTGACCTTTTCCAACATTAGACTTATTCATATATTTTAACGGCGGGTCTCATAAGGATGATTAACTCATCCCTTCTTCATTAC‑‑‑AA‑‑‑AAAAGGCCGACAGCTTgggg                                                                                                      >  1:478762/1‑140 (MQ=255)
                                      atgaatgaTTTTCTTAATTAAATTGATTAATTTTTTGACCTTTTCCAACATTAGACTTATTCATATATTTTAACGGCGGGTCTCATAATGATGATTAACTCATCCCTTCTTCATTAC‑‑‑AA‑‑‑AAAAGGCCGCGAGCTTGGGAgg                                                                                                    <  2:697796/141‑1 (MQ=255)
                                        gaatgaTTTTCTTAATTAAATTGATTAATTTTTTGACCTTTTCCAACATTAGACTTATTCATATATTTTAACGTCGGGTCTCATAATGATGATTAACTCATCCCTTCTTCATTAC‑‑‑AA‑‑‑AAAAGGCCGCGAGCTTGGGAgggg                                                                                                  >  4:187738/1‑141 (MQ=255)
                                           tgaTTTTCTTAATTAAATTGATTAATTTTTTGACCTTTTCCAACATTAGACTTATTCATATATTTTAACGGCGGGTCTCATAATGATGATTAACTCATCCCTTCTTCATTAC‑‑‑AA‑‑‑AAAAGGCCGCGAGCTTGGGAGGGGCTc                                                                                               <  1:316352/141‑1 (MQ=255)
                                            gaTTTTCTTAATTAAATTGATTAATTTTTTGACCTTTTCCAACATTAGACTTATTCATATATTTTAACGGCGGGTCTCATTATGATGATTAACTCATTCCTTCTTTATTAC‑‑‑AA‑‑‑AAAAAGCCGCGAGCTTGGGAGGGGCTCa                                                                                              >  1:1077786/1‑141 (MQ=255)
                                            gaTTTTCTTAATTAAATTGATTAATTTTTTGACCTTTTCCAACATTAGACTTATTCATATATTTTAACGGCGGGTCTCATAATGATGATTAACTCATCCCTTCTTCATTAC‑‑‑AA‑‑‑AAAAGGCCGCGAGCTTGGGAGGGGGTCa                                                                                              >  1:797806/1‑141 (MQ=255)
                                            gaTTTTCTTAATTAAATTGATTAATTTTTTGACCTTTTCCAACATTAGACTTATTCATATATTTTAACGGCGGGTCTCATAATGATGATTAACTCATCCCTTCTTCATTAC‑‑‑AA‑‑‑AAAAGGCCGCGAGCTTGGGAGGGGCTCa                                                                                              >  3:250795/1‑141 (MQ=255)
                                             aTTTTCTTAATTAAATTGATTAATTTTTTGACCTTTTCCAACATTAGACTTATTCATATATTTTAACGGCGGGTCTCATAATGATGATTAACTCATCCCTTCTTCATTAC‑‑‑AA‑‑‑AAAAGGCCGCGAGCTTGGGAGGGGCTCAc                                                                                             >  1:489898/1‑141 (MQ=255)
                                              ttttCTTAATTAAATTGATTAATTTTTTGACATTTCCCAACATTAGACTTATTCATATATTTTAACGGCGGGGCTCATAATGATGATTAACTCATCCCTTCTTCATTAC‑‑‑AA‑‑‑AAAAGGCCGCGAGCTTGGGAGGGGCTCACg                                                                                            <  2:557207/141‑1 (MQ=255)
                                               tttCTTAATTAAATTGATTAATTTTTTTTTTTTTTTTAACATTAGACTTATTCATATATTTTAACGGCGGGTCTCATAATTATGATTAACTCATTCCTTCTTCATTAC‑‑‑AA‑‑‑AAAATGCCTCTATCTTTGGAGGGGTTCActa                                                                                           >  4:77462/1‑139 (MQ=255)
                                               tttCTTAATTAAATTGATTAATTTTTTGACCTTTTCCAACATTAGACTTATTCATATATTTTAACGGCGGGTCTCATAATGATGATTAACTCATCCCTTCTTCATTAC‑‑‑AA‑‑‑AAAAGGCCGCGAGCTTGGGAGGGGCTCACGa                                                                                           <  1:628717/141‑1 (MQ=255)
                                                 tCTTAATTAAATTGATTAATTTTTTGACCTTTTCCAACATTAGACTTATTCATATATTTTAACGGCGGGTCTCATAATGATGATTAACTCATCCCTTCTTCATTAC‑‑‑AA‑‑‑AAAAGGCCGCGAGCTTGGGAGGGGCTCACGAcc                                                                                         >  1:991599/1‑141 (MQ=255)
                                                 tCTTAATTAAATTGATTAATTTTTTGACCTTTTCCAACATTAGACTTATTCATATATTTTAACGGCGGGTCTCATAATGATGATTAACTCATCCCCTCTTCATTCC‑‑‑AA‑‑‑AAAAGGCCGCGAGCTTGGGAGGGGCCCCCGAcc                                                                                         >  2:336539/1‑141 (MQ=255)
                                                  cttacttaAATTGATTAATTTTTTGACCTTTTCCAACATTAGACTTATCCATATATTTTAACGGCGGGTCTCATAATGATGATTAACTCATCCCTTCTTCATTAC‑‑‑AA‑‑‑AAAAGGCCGCGAGCTTGGGAGGGGCTCACGACCa                                                                                        <  4:412640/141‑1 (MQ=255)
                                                       ttaaATTGATTAATTTTTTGACCTTTTCCAACATTAGACTTATTCATATATTTTAACGGCGGGTCTCATAATGATGATTAACTCATCCCTTCTTCATTAC‑‑‑AA‑‑‑AAAAGGCCGCGAGCTTGGGAGGGGCTCACGACCATACtt                                                                                   <  4:443600/141‑1 (MQ=255)
                                                       ttaaATTGATTAATTTTTTGACCTTTTCCAACATTAGACTTATTCATATATTTTAACGGCGGGTCTCATAATGATGATTAACTCATCCCTTCTTCATCAC‑‑‑AA‑‑‑AAAAGGCCGCGAGCTTGGGAGGGGCTCACGACCATACtt                                                                                   <  1:744671/141‑1 (MQ=255)
                                                           aTTGATTAATTTTTTGACCTTTTCCAACATTAGACTTATTCATATATTTTAACGGCGGGTCTCATAATGATGATTAACTCATCCCTTCTTCATTAC‑‑‑AA‑‑‑AAAAGGCCGCGAGCTTGGGAGGGGCTCCCGACCATACttatta                                                                               >  2:988018/1‑141 (MQ=255)
                                                              gATTAATTTTTTGACCTTTTCCAACATTAGACTTATTCATATATTTTAACGGCGGGTCTCATAATGATGATTAACTCATCCCTTCTTCATTAC‑‑‑AA‑‑‑AAAAGGCCGCGAGCTTGGGAGGGGCTCACGACCATACttattatta                                                                            >  1:536783/1‑141 (MQ=255)
                                                              gATTAATTTTTTGACCTTTTCCAACATTAGACTTATTCATATATTTTAACGGCGGGTCTCATAATGATGATTAACTCATCCCTTCTTCATTAC‑‑‑AA‑‑‑AAAAGGCCGCGAGCTTGGGAGGGGCTCACGACCATACttattatta                                                                            <  2:151055/141‑1 (MQ=255)
                                                                ttAATTTTTTGACCTTTTCCAACATTAGACTTATTCATATATTTTAACGGCGGGTTTCATAATGATGATTAACTCCTTCCTTCTTTATTAC‑‑‑CA‑‑‑AAAAGGCCGGGAGCTTGGGAGGGGGTGACGAATATACTTATTATTAAg                                                                          >  1:710091/1‑141 (MQ=255)
                                                                ttAATTTTTTGACCTTTTCCAACATTAGACTTATTCATATATTTTAACGGCGGGTCTCATTATGATGATTAACTCATCCCTTCTTCATTAC‑‑‑AA‑‑‑AAAAGGCCGCGAGCTTGGGAGGGGCTCACGACCATACTTATTATTAAg                                                                          >  3:376165/1‑141 (MQ=255)
                                                                  aaTTTTTTGACCTTTTCCAACATTAGACTTATTCATATATTTTAACGGCGGGTCTCATAATGATGATTAACTCATCCCTTCTTCATTAC‑‑‑AA‑‑‑AAAAGGCCGCGAGCTTGGGAGGGGCTCACGACCATACTTATTATTAAggg                                                                        <  2:621908/141‑1 (MQ=255)
                                                                  aaTTTTTTGACCTTTTCCAACATTAGACTTATTCATATATTTTAACGGCGGGTCTCATAATGATGATTAACTCATCCCTTCTTCATTAC‑‑‑AA‑‑‑AAAAGGCCGCGAGCTTGGGAGGGGCTCACGACCATACTTATTATTAAggg                                                                        <  4:419790/141‑1 (MQ=255)
                                                                   aTTTTTTGACCTTTTCCAACATTAGACTTATTCATATATTTTAACGGCGGGTCTCATAATGATGATTAACTCATCCCTTCTTCATTAC‑‑‑AA‑‑‑AAAAGGCCGCGAGCTTGGGAGGGGCTCACGACCATACTTATTATTAAGGGa                                                                       >  1:321152/1‑141 (MQ=255)
                                                                   aTTTTTTGACCTTTTCCAACATTAGACTTATTCATATATTTTAACGGCGGGTCTCATAATGATGATTAACTCATCCCTTCTTCATTAC‑‑‑AA‑‑‑AAAAGGCCGCGAGCTTGGGAGGGGCTCACGACCATACTTATTATTAAGGGa                                                                       <  3:207838/141‑1 (MQ=255)
                                                                    ttttttGACCTTTTCCAACATTAGACTTATTCATATATTTTAACGGCGGGTCTCATAATGATGATTAACTCATCCCTTCTTCATTAC‑‑‑AA‑‑‑AAAAGGCCGCGAGCTTGGGAGGGGCTCACGTCCATACTTATTATTAAGGGaa                                                                      <  2:405402/141‑1 (MQ=255)
                                                                       tttGACCTTTTCCAACATTAGACTTATTCATATATTTTAACGGCGGGTCTCATAATGATGATTAACTCATCCCTTCTTCATTAC‑‑‑AA‑‑‑AAAAGGCCGCGAGCTTGGGAGGGGCTCACGACCATACTTATTATTAAGGGAATGt                                                                   >  1:849176/1‑141 (MQ=255)
                                                                          gACCTTTTCCAACATTAGACTTATTCATATATTTTAACGGCGGGTCTCATAATGATGATTAACTCATCCCTTCTTCATTAC‑‑‑AA‑‑‑AAAAGGCCGCGAGCTTGGGAGGGGCTCACGACCATACTTATTATTAAGGGAATGtttt                                                                >  1:707361/1‑141 (MQ=255)
                                                                           aCCTTTTGCAACATTAGACTTATTCATATATTTTAACGGCGGGTCTCATAATGATGATTAACTCATCCCTTCTTCATTAC‑‑‑AA‑‑‑AAAAGGCCGCGAGCTTGGGAGGGGCTCACGACCATACTTATTATTAAGGGAATGTTTTa                                                               >  1:858096/1‑141 (MQ=255)
                                                                             cTTTTCCAACATTAGACTTATTCATATATTTTAACGGCGGGTCTCATAATGATGCTTAACTCCTCCCTTCTTCCTTAC‑‑‑AA‑‑‑AAAAGGCCGCGAGCTTGGGAGGGGCTCACGACCATACTTATTATTCAGGGAATGTTTTAcc                                                             >  2:148549/1‑140 (MQ=255)
                                                                              ttttCCAACATTAGACTTATTCATATATTTTAACGGCGGGTCTCATAATGATGATTAACTCATCCCTTCTTCATTAC‑‑‑AA‑‑‑AAAAGGCCGCGAGCTTGGGAGGGGCTCACGACCATACTTATTATTAAGGGAATGTTTTACAg                                                            <  4:81448/141‑1 (MQ=255)
                                                                              ttttCCAACATTAGACTTATTCATATATTTTAACGGCGGGTCTCATAATGATGATTAACTCATCCCTTCTTCATTAC‑‑‑AA‑‑‑AAAAGGCCGCGAGCTTGGGAGGGGCTCACGACCATACTTATTATTAAGGGAATGTTTTACAg                                                            <  2:471546/141‑1 (MQ=255)
                                                                               tttCCAACATTAGACTTATTCATATATTTTAACGGTGGGTCTCATAATGAGGATTAACTCTCCCCTTCTTTATTACAATAA‑‑‑AAAAGGCCGCGAGCTTGGGAGGGGCTCACGACCATACTTATTATTAAGGGAATGTTTTAc                                                              <  2:654532/141‑1 (MQ=255)
                                                                                  ccAACATTAGACTTATTCATATATTTTAACGGCGGGTCTCATAATGATGATTAACTCATCCCTTCTTCATTAC‑‑‑AA‑‑‑AAAAGGCCGCGAGCTTGGGAGGGGCTCACGACCATACTTATTATTAAGGGAATGTTTTACAGTTAt                                                        <  3:65808/141‑1 (MQ=255)
                                                                                   cAACATTAGACTTATTCATATATTTTAACGGCGGGTCTCATAATGATGATTAACTCATCCCTTCTTCATTAC‑‑‑AA‑‑‑AAAAGGCCGCGAGCTTGGGAGGGGCTCACGACCATACTTATTATTAAGGGAATGTTTTACAGTTAtt                                                       >  1:240351/1‑141 (MQ=255)
                                                                                   cAACATTAGACTTATTCATATATTTTAACGGCGGGTCTCATAATGATGATTAACTCATCCCTTCTTCATTAC‑‑‑AA‑‑‑AAAAGGCCGCGAGCTTGGGAGGGGCTCACGACCATACTTATTATTAAGGGAATGTTTTACAGTTAtt                                                       <  1:241394/141‑1 (MQ=255)
                                                                                    aaCATTAGACTTATTCATATATTTTAACGGCGGGTCTCATAATGATGATTAACTCATCCCTTCTTCATTAC‑‑‑AA‑‑‑AAAAGGCCGCGAGCTTGGGAGGGGCTCACGACCATACTTATTATTAAGGGAATGTTTTACAGTTAttt                                                      >  1:354682/1‑141 (MQ=255)
                                                                                       aTTAGACTTATTCATATATTTTAACGGCGGGTCTCATAATGATGATTAACTCATCCCTTCTTCATTAC‑‑‑AA‑‑‑AAAAGGCCGCGAGCTTGGGAGGGGCTCACGACCATACTTATTATTAAGGGAATGTTTTACAGTTAtttttt                                                   >  1:238795/1‑141 (MQ=255)
                                                                                          aGACTTATTCATATATTTTAACGGCGGGTCTCATAATGATGATTAACTCATCCCTTCTTCATTAC‑‑‑AA‑‑‑AAAAGGCCGCGAGCTTGGGAGGGGCTCACGACCATACTTATTATTAAGGGAATGTTTTACAGTTATTTTTTCaa                                                >  2:199688/1‑141 (MQ=255)
                                                                                           gACTTATTCATATATTTTAACGGCGGGTCTCATAATGATGATTAACTCATCCCTTCTTTATTAC‑‑‑AA‑‑‑AAAAGGCCGCGGGGTTGGGAGGGGCTCACGACCATACTTATTTTTAAGGGAATGTTTTACAGTTATTTTTTCAAt                                               >  1:214958/1‑141 (MQ=255)
                                                                                           gACTTATTCATATATTTTAACGGCGGGTCTCATAATGATGATTAACTCATCCCTTCTTCATTAC‑‑‑AA‑‑‑AAAAGGCCGCGAGCTTGGGAGGGGCTCACGACCATACTTATTATTAAGGGAATGTTTTACAGTTATTTTTTCAAt                                               >  3:84766/1‑141 (MQ=255)
                                                                                           gACTTATTCATATATTTTAACGGCGGGTCTCATAATGATGATTAACTCATCCCCTCTTCATTAC‑‑‑CA‑‑‑AAAAGGCCGCGAGCTTGGGAGGGGCTCACGACCACACTTATTATTAAGGGAATGGTTTACAGTTATTTTTTCAAt                                               >  1:985106/1‑141 (MQ=255)
                                                                                            aCTTATTCATATATTTTAACGGCGGGTCTCATAATGATGATTAACTCATCCCTTCTTCATTAC‑‑‑AA‑‑‑AAAAGGCCGCGAGCTTGGGAGGGGCTCACGACCATACTTATTATTAAGGGAATGTTTTACAGTTATTTTTTCAATc                                              <  2:946112/141‑1 (MQ=255)
                                                                                            aCTTATTCATATATTTTAACGGCGGGTCTCATAATGATGATTAACTCATCCCTTCTTCATTAC‑‑‑AA‑‑‑AAAAGGCCGCGAGCTTGGGAGGGGCTCACGACCATACTTATTATTAAGGGAATGTTTTACAGTTATTTTTTCAATc                                              <  4:330587/141‑1 (MQ=255)
                                                                                              ttATTCATATATTTTAACGGCGGGTCTCATAATGATGATTAACTCATCCCTTCTTCATTAC‑‑‑AA‑‑‑AAAAGGCCGCGAGCTTGGGAGGGGCTCACGACCATACTTATTATTAAGGGAATGTTTTACAGTTATTTTTTCAATCTa                                            <  1:134135/141‑1 (MQ=255)
                                                                                                aTTCATATATTTTAACGGCGGGTCTCATAATGATGATTAACTCATCCCTTCTTCATTAC‑‑‑AA‑‑‑AAAAGGCCGCGAGCTTGGGAGGGGCTCACGACCATACTTATTATTAAGGGAATGTTTTACAGTTATTTTTTCAATCTAtt                                          >  2:349512/1‑141 (MQ=255)
                                                                                                       tatTTTAACGGCGGGTCTCATAATGATGATTAACTCATCCCTTCTTCATTAC‑‑‑AA‑‑‑AAAAGGCCGCGAGCTTGGGAGGGGCTCACGACCATACTTATTATTAAGGGAATGTTTTACAGTTATTTTTTCAATCTATTTTTgggg                                   >  4:64063/1‑141 (MQ=255)
                                                                                                         ttttAACGGCGGGTCTCATAATGATGATTAACTCATCCCTTCTTCATTAC‑‑‑AA‑‑‑AAAAGGCCGCGAGCTTGGGAGGGGCTCACGACCATACTTATTATTAAGGGAATGTTTTACAGTTATTTTTGCGATCTATTTTTGGGGAt                                 >  1:9032/1‑141 (MQ=255)
                                                                                                          tttAACGGCGGGTCTGATAATGATGATTAACTCATCCCTTCTTCATTAC‑‑‑AA‑‑‑AAAAGGCCGCGAGCTTGGGAGGGGCTCACGACCATACTTATTATTATGGGAATGTTTTACAGTTATTTTTTCAATCTATTTTTGGGGATg                                <  2:136157/141‑1 (MQ=255)
                                                                                                           ttAACGGCTGGTTTCATAATTATTATTATCTCATCCCTTCTTCATTAC‑‑‑AA‑‑‑AAAAGGCCGCGAGCTTGGGAGGGTCTCTCTACCATATTTATTTTTAAGTGAATGTTTTACAGTTATTTTTTCAATCTATTTTTGGGGATGt                               <  2:915368/141‑1 (MQ=255)
                                                                                                           ttAACGGCGGGTCTCATAATGATGATTAACTCATCCCTTATTCATTAC‑‑‑AA‑‑‑AAAAGGCCGCGAGCTTGGGAGGGGCTCACGACCATACTTATTATTAAGGGAATGTTTTACAGTTATTTTTTCAATCTATTTTTGGGGATGt                               <  2:581431/141‑1 (MQ=255)
                                                                                                            tAACGGCGGGTCTCATAATGATGATTAACTCATCCCTTCTTCATTAC‑‑‑AA‑‑‑AAAAGGCCGCGAGCTTGGGAGGGGCTCACGACCATACTTATTATTAAGGGAATGTTTTACAGTTATTTTTTCAATCTATTTTTGGGGATGtt                              <  2:991599/141‑1 (MQ=255)
                                                                                                            tAACGGCGGGTCTCATAATGATGATTAACTCATCACTTCTTCATTAC‑‑‑AA‑‑‑AAAAGGCCGCGAGCTTGGGAGGGGCTCACGACCATACTTATTATTAAGGGAATGTTTTACAGTTATTTTTTCAATCTATTTTTGGGGATGtt                              <  4:219169/141‑1 (MQ=255)
                                                                                                            tAACGGCGGGTCTAATAATGAGGATTAACTCATCCCTTCTTCATTAC‑‑‑AA‑‑‑AAAAGGCCGCGAGCTTGGAAGGGGCTTACGACCATACTTATTATTAAGGGAATGTTTTACAGTTATTTTGTCAATCTATTTTTGGGgttgtt                              <  4:199611/141‑1 (MQ=255)
                                                                                                              aCGGCGGGTCTCATAATGATGATTAACTCATCCCTTCTTCATTAC‑‑‑AA‑‑‑AAAAGGCCGCGAGCTTGGGAGGGGCTCACGACCATACTTATTATTAAGGGAATGTTTTACAGTTATTTTTTCAATCTATTTTTGGGGATGttat                            >  1:777314/1‑141 (MQ=255)
                                                                                                               cggcggGTCTCATAATGATGATTAACTCATCCCTTCTTCATTAC‑‑‑AA‑‑‑AAAAGGCCGCGAGCTTGGGAGGGGCTCACGACCATACTTTTTATTAAGGGAATGTTTTACAGTTATTTTTTCAATCTAGTTTTGGGGATGttatt                           >  1:682160/1‑141 (MQ=255)
                                                                                                                ggcggGTCTCATAATGATGATTAACTCATCCCTTCTTCATTAC‑‑‑AA‑‑‑AAAAGGCCGCGAGCTTGGGAGGGGCTCACGACCATACTTATTATTAAGGGAATGTTTTACAGTTATTTTTTCAATCTATTTTTGGGGATGttatta                          <  2:787423/141‑1 (MQ=255)
                                                                                                                  cggGTCTCATAATGATGATTAACTCATCCCTTCTTCATTAC‑‑‑AA‑‑‑AAAAGGCCGCGAGCTTGGGAGGGGCTCACGACCATACTTATTATTAAGGGAATGTTTTACAGTTATTTTTTCAATCTATTTTTGGGGATGTTattaat                        >  1:238780/1‑141 (MQ=255)
                                                                                                                    tttctcATAATGAGGCTTATCTCTCCCCTTTTTTATTACAATAA‑‑‑AAAAGGCCTCGAGGTTGGGAGGGGCTCACTACCATACTTATTATTAAGGGAATGTTTTACAGTTATTTTTTCAATCTATTTTTTGGGATGTTattaa                         <  4:337796/139‑1 (MQ=255)
                                                                                                                    ggTCTCATAATGAGGATTAACTCTCACCTTCTTTATTAA‑‑‑AATATAAAAGGCCTCGAGCTTGGGAGGGGCTCACGACCATACTTATTATTAAGGGAATGTTTTACAGTTATTTTTTCAATCTATTTTTGGGGATGTTattaa                         <  4:337794/141‑1 (MQ=255)
                                                                                                                       ctcATAATGATGATTAACTCATCCCTTCTTCATTAC‑‑‑AA‑‑‑AAAAGGCCGCGAGCTTGGGAGGGGCTCACGACCATACTTATTATTAAGGGAATGTTTTACAGTTATTTTTTCAATCTATTTTTGGGGATGTTATTAATTATGa                   <  2:489898/141‑1 (MQ=255)
                                                                                                                       cccATAATGATGATTAACTCATCCCCTCTTCATTAC‑‑‑AA‑‑‑AAAAGGCCGCGAGGTTGGGAGGGGCTCACGACCATACTTATTATTAAGGGAATGTTTTACAGTTATTTTTTCAATCTATTTTTGGGGATGTTATTAATTATGa                   >  4:239164/3‑141 (MQ=255)
                                                                                                                        tcATAATGATGATTAACTCATCCCTTCTTCATTAC‑‑‑AA‑‑‑AAAAGGCCGCGAGCTTGGGAGGGGCTCACGACCATACTTATTATTAAGGGAATGTTTTACAGTTATTTTTTCAATCTATTTTTGGGGATGTTATTAATTATGaa                  <  1:474285/141‑1 (MQ=255)
                                                                                                                         cATAATGATGATTAACTCATCCCTTCTTCATTAC‑‑‑AA‑‑‑AAAAGGCCGCGAGCTTGGGAGGGGCTCACGACCATACTTATTATTAAGGGAATGTTTTACAGTTATTTTTTCAATCTATTTTTGGGGATGTTATTAATTATGaaa                 >  3:410995/1‑141 (MQ=255)
                                                                                                                          aTAATGATGATTAACTCATCCCTTCTTCATTAC‑‑‑AA‑‑‑AAAAGGCCGCGAGCTTGGGAGGGGCTCACGACCATACTTATTATTAAGGGAATGTTTTACAGTTATTTTTTCAATCTATTTTTGGGGATGTTATTAATTATGaaaa                <  2:708458/141‑1 (MQ=255)
                                                                                                                           tAATGATGATTAACTCATCATTTCTTCATTAC‑‑‑AA‑‑‑AAAAGGCCGCGAGCTTGGGAGGGGCTCACGACCATACTTATTATTAAGGGAATGTTTTACAGTTATTTTTTCAATCTATTTTTGGGGATGTTATTAATTATGaaaaa               <  3:229937/141‑1 (MQ=255)
                                                                                                                              ggatgatTAGCGCATGACTTCGTCATTAC‑‑‑AA‑‑‑AAAAGGCCGCGAGCTTGGGAGGGGCTCACGACCATACTTATTATTAAGGGAATGTTTTACAGTTATTTTTTCAATCTATTTTTGGGGATGTTATTAATTATGAAAAAAAt            <  2:277126/140‑1 (MQ=255)
                                                                                                                               gatgatTAACTCATCCCTTCTTCATTAC‑‑‑AA‑‑‑AAAAGGCCGCGAGCTTGGGAGGGGCTCACGACCATACTTATTATTAAGGGAATGTTTTACAGTTATTTTTTCAATCTATTTTTGGGGATGTTATTAATTATGAAAAAAAtt           <  2:777314/141‑1 (MQ=255)
                                                                                                                                 tgatTAACTCATCCCTTCTTCATTAC‑‑‑AA‑‑‑AAAAGGCCGCGAGCTTGGGAGGGGCTCACGACCATACTTATTATTAAGGGAATGTTTTACAGTTATTTTTTCAATCTATTTTTGGGGATGTTATTAATTATGAAAAAAAtttt         >  4:419586/1‑141 (MQ=255)
                                                                                                                                   atTAACTCATCCCTTCTTCATTAC‑‑‑AA‑‑‑AAAAGGCCGCGAGCTTGGGAGGGGCTCACGACCATACTTATTATTAAGGGAATGTTTTACAGTTATTTTTTCAATCTATTTTTGGGGATGTTATTAATTATGAAAAAAATtttat       >  1:150020/1‑141 (MQ=255)
                                                                                                                                      aaCTCATCCCTTCTTCATTAC‑‑‑AA‑‑‑AAAAGGCCGCGAGCTTGGGAGGGGCTCACGACCATACTTATTATTAAGGGAATGTTTTACAGTTATTTTTTCAATCTATTTTTGGGGATGTTATTAATTATGAAAAAAATtttattta    <  3:298194/141‑1 (MQ=255)
                                                                                                                                        cTCATCCCTTCTTCATTAC‑‑‑AA‑‑‑AAAAGGCCGCGAGCTTGGGAGGGGCTCACGACCATACTTATTATTAAGGGAATGTTTTACAGTTATTTTTTCAATCTATTTTTGGGGATGTTATTAATTATGAAAAAAATTTTATTTATc  >  4:429961/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                               
ATAGATAACTCAGTAAGAACCCATTTCGCCCTTTGCAAATGAATGATTTTCTTAATTAAATTGATTAATTTTTTGACCTTTTCCAACATTAGACTTATTCATATATTTTAACGGCGGGTCTCATAATGATGATTAACTCATCCCTTCTTCATTAC‑‑‑AA‑‑‑AAAAGGCCGCGAGCTTGGGAGGGGCTCACGACCATACTTATTATTAAGGGAATGTTTTACAGTTATTTTTTCAATCTATTTTTGGGGATGTTATTAATTATGAAAAAAATTTTATTTATC  >  USA300TCH1516_ALE/2146701‑2146977

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: