BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	2,189,877	G→T	5.5%	R100R (CGA→AGA)	alr1_2 ←	Alanine racemase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,189,877	0	G	T	5.5%	457.7 / 9.8	139	R100R (CGA→AGA)	alr1_2	Alanine racemase 1
Reads supporting (aligned to +/- strand): ref base G (66/64); new base T (3/5); total (70/69)
Fisher's exact test for biased strand distribution p-value = 7.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.06e-01

Only 100 of 143 total aligned reads displayed.

CGTTTTAGTATCTTTAATACCTAAACGTCCCATTCCTGTATCTAATTTAATGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCTAAGTTCTATCGCTTCATCTAACGTTGCTACAGCAAAAAATGTGGCGCCATTTTCCATTAAAT  >  USA300TCH1516_ALE/2189737‑2190017

cGTTTTAGTATCTTTAATACCTAAACGTCCCATTCCTGTATCTAATTTAATGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCg                                                                                                                                              >  1:671289/1‑141 (MQ=255)

    ttAGTATCTTTAATACCTAAACGTCCCATTCCTGTATCTAATTTAATGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGgtgt                                                                                                                                          <  1:100145/141‑1 (MQ=255)

    ttAGTATCTTTAATACCTAAACGTCCCATTCCTGTATCTAATTTAATGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGgtgt                                                                                                                                          <  1:396868/141‑1 (MQ=255)

      aGTATCTTTAATACCTAAACGTCCCATTCCTGTATCTAATTTAATGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGtgttg                                                                                                                                        >  2:607231/1‑141 (MQ=255)

      aGTATCTTTAATACCTAAACGTCCCATTACTGTATCTAATTTAATGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGtgttg                                                                                                                                        <  1:446819/141‑1 (MQ=255)

        tATCTTTAATACCTAAACGTCCCATTCCTGTATCTAATTTAATGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTa                                                                                                                                      >  3:417124/1‑141 (MQ=255)

        tATCTTTAATACCTAAACGTCCCATTCCTGTATCTAATTTAATGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTa                                                                                                                                      >  3:450299/1‑141 (MQ=255)

         aTCTTTAATACCTAAACGTCCCATTCCTGTATCTAATTTAATGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTAt                                                                                                                                     <  2:159292/141‑1 (MQ=255)

            tttAATACCTAAACGTCCCATTCCTGTATCTAATTTAATGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGc                                                                                                                                  <  3:305241/141‑1 (MQ=255)

            tttAATACCTAAACGTCCCATTCCTGTATCTAATTTAATGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGc                                                                                                                                  <  2:468312/141‑1 (MQ=255)

             ttAATACCTAAACGTCCCATTCCTGTATCTAATTTAATGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCTGTGTTGTATCGCt                                                                                                                                 <  1:758923/141‑1 (MQ=255)

                aTACCTAAACGTCCCATTCCTGTATCTAATTTAATGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTa                                                                                                                              >  1:746249/1‑141 (MQ=255)

                aTACCTAAACGTCCCATTCCTGTATCTAATTTAATGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTa                                                                                                                              >  1:611161/1‑141 (MQ=255)

                 tACCTAAACGTCCCATTCCTGTATCTAATTTAATGTGCAACCATAACTTTTTTTTTTTTTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTAt                                                                                                                             >  4:49165/1‑141 (MQ=255)

                 tACCTAAACGTCCCATTCCTGTATCTAATTTAATGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTAt                                                                                                                             >  4:51665/1‑141 (MQ=255)

                  aCCTAAACGTCCCATTCCTGTATCTAATTTAATGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATc                                                                                                                            <  2:287187/141‑1 (MQ=255)

                     tAAACGTCCCATTCCTGTATCTAATTTAATGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAat                                                                                                                         >  4:111944/1‑141 (MQ=255)

                     tAAACGTCCCATTCCTGTATCTAATTTAATGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAat                                                                                                                         >  4:320453/1‑141 (MQ=255)

                     tAAACGTCCCATTCCTGTATCTAATTTAATGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAat                                                                                                                         >  1:771350/1‑141 (MQ=255)

                     tAAACGTCCCATTCCTGTATCTAATTTAATGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAat                                                                                                                         >  4:111999/1‑141 (MQ=255)

                       aaCGTCCCATTCCTGTATCTAATTTAATGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAatat                                                                                                                       >  2:699224/1‑141 (MQ=255)

                       aaCGTCCCATTCCTGTATCTAATTTAATGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAatat                                                                                                                       <  2:395074/141‑1 (MQ=255)

                        aCGTCCCATTCCTGTATCTAATTTAATGTGCAACCATTACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATc                                                                                                                      <  4:377217/141‑1 (MQ=255)

                        aCGTCCCATTCCTGTATCTAATTTAATGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATc                                                                                                                      <  3:281923/141‑1 (MQ=255)

                        aCGTCCCATGCCTGTAGCTAATGTAATGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTATACGGAACCGTTAAGGCGACTCTGTGTTGTATCGCTTTATCAATATc                                                                                                                      <  2:99019/141‑1 (MQ=255)

                           tCCCATTCCTGTTTCTAATTTAATGTGCATCCATAACCTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCTACCACTTTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCttt                                                                                                                   <  1:955494/141‑1 (MQ=255)

                           tCCCATTCCTGTATCTAATTTAATGTGCAACCATAACTTTTTCTCTTGCTCACCAGAGATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCttt                                                                                                                   <  2:994452/141‑1 (MQ=255)

                           tCCCATTCCTGTATCTAATTTAATGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCttt                                                                                                                   <  3:435087/141‑1 (MQ=255)

                           tCCCATTCCTGTATCTAATTTAATGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCttt                                                                                                                   >  1:533294/1‑141 (MQ=255)

                           tCCCATTCCTGTATCTAATTTAATGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCttt                                                                                                                   <  3:435273/141‑1 (MQ=255)

                             ccATTCCTGTATCTAATTTAATGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCTGTGTTGTATCGCTTTATCAATATCTTTAg                                                                                                                 >  2:340739/1‑141 (MQ=255)

                             ccATTCCTGTATCTAATTTAATGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCTGTGTTGTATCGCTTTATCAATATCTTTAg                                                                                                                 >  2:340681/1‑141 (MQ=255)

                                   cTGTATCTAATTTAATGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTa                                                                                                           <  3:394049/141‑1 (MQ=255)

                                      tATCTAATTTAATGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAaca                                                                                                        >  3:314263/1‑141 (MQ=255)

                                        tCTAATTTAATGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAacaca                                                                                                      >  1:630352/1‑141 (MQ=255)

                                         cTAATTTAATGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAacacac                                                                                                     >  1:349221/1‑141 (MQ=255)

                                            aTTTAATGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTa                                                                                                  >  1:595682/1‑141 (MQ=255)

                                               tAATGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCCCTGTTTAGACGGAACCGTTAAGGCAACTCCGTGTTGTATCGCTTTCACAATATCTTTAGCTGGTAACACACCTATGa                                                                                               >  4:414516/1‑141 (MQ=255)

                                               tAATGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGa                                                                                               >  3:209430/1‑141 (MQ=255)

                                                aaTGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGAc                                                                                              >  3:85448/1‑141 (MQ=255)

                                                 aTGTGCACCCATCACTTTTTCTCTTGCTCAGCAGAAATGTTTTTACTTGCATCTTTCAACCACTGTTTAGACGGCACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACt                                                                                             <  4:201380/141‑1 (MQ=255)

                                                 aTGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACt                                                                                             <  2:30441/141‑1 (MQ=255)

                                                   gtgCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTaa                                                                                           <  4:217911/141‑1 (MQ=255)

                                                   gtgCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTaa                                                                                           >  2:883855/1‑141 (MQ=255)

                                                   gtgCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTaa                                                                                           <  4:298408/141‑1 (MQ=255)

                                                    tgCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTaaa                                                                                          >  1:767349/1‑141 (MQ=255)

                                                            tcaCTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGc                                                                                  <  1:661289/139‑1 (MQ=255)

                                                              aCTTTTTCTCTTGCTCACCAGAAATGTTTTTTTTTTTTTTTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTATCTGGTAACACACCTAATACTAAAATTTTAGCAg                                                                                >  2:745612/1‑141 (MQ=255)

                                                              aCTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAg                                                                                <  3:187723/141‑1 (MQ=255)

                                                              aCTTTTTCTCTTGCTCACCAGAAACGTTTTTAATTGCTTCTTTCCACCACTGTTTAGACGGAACCGTTTAGGCAACTCGGTGTTGTATCGCTGTATCAACAGCCTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAg                                                                                >  4:122622/1‑141 (MQ=255)

                                                              aCTTTTTCTCTGGCTCACCAGAAATTTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAg                                                                                <  4:335394/141‑1 (MQ=255)

                                                                   ttCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATc                                                                           <  1:472743/141‑1 (MQ=255)

                                                                    tctcTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATcc                                                                          >  1:559450/1‑141 (MQ=255)

                                                                      tctTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCa                                                                        >  2:528356/1‑141 (MQ=255)

                                                                         tGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGc                                                                     >  1:260132/1‑141 (MQ=255)

                                                                         tGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGc                                                                     >  1:1055452/1‑141 (MQ=255)

                                                                          gCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCa                                                                    <  2:374854/141‑1 (MQ=255)

                                                                          gCTCACCAGAAATGTATCGACGTTCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCa                                                                    <  1:9762/141‑1 (MQ=255)

                                                                          ccTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGGCGGAACCGCTAAGGCACCTCGGTGTTGTATCCCTTTATCAATATCCTTCGCTGGTAACACACCTAAGGCGACAATTTTAGCATTAATCCCATGCa                                                                    <  2:759171/140‑1 (MQ=255)

                                                                              aCCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCt                                                                >  2:785458/1‑141 (MQ=255)

                                                                               ccAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCTa                                                               <  1:487909/141‑1 (MQ=255)

                                                                                 aGAAATGTTTTTTTTTTTTTTTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCTAAg                                                             >  2:443264/1‑141 (MQ=255)

                                                                                 aGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCTAAg                                                             >  2:828717/1‑141 (MQ=255)

                                                                                   aaaTGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCTAAGtt                                                           >  2:158953/1‑141 (MQ=255)

                                                                                     aTGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCTAAGTTCt                                                         >  1:687823/1‑141 (MQ=255)

                                                                                       gTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCTAAGTTCTAt                                                       <  1:128063/141‑1 (MQ=255)

                                                                                         ttttAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCTGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCTAAGTTCTATCg                                                     >  3:137040/1‑141 (MQ=255)

                                                                                          tttAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCTAAGTTCTATCGc                                                    <  1:961898/141‑1 (MQ=255)

                                                                                          tttAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCTAAGTTCTATCGc                                                    <  1:155502/141‑1 (MQ=255)

                                                                                           ttAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATCTTAGCAGTAATCCCATGCATTCTAAGTTCTATCGCt                                                   <  2:255673/141‑1 (MQ=255)

                                                                                              aTTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCTAAGTTCTATCGCTTCa                                                <  3:410379/141‑1 (MQ=255)

                                                                                               ttGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCTAAGTTCTATCGCTTCAt                                               <  4:115877/141‑1 (MQ=255)

                                                                                                 gCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCTAAGTTCTATCGCTTCATCt                                             >  1:552983/1‑141 (MQ=255)

                                                                                                 gCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCTAAGTTCTATCGCTTCATCt                                             >  1:625694/1‑141 (MQ=255)

                                                                                                 gCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGAAACACCCCTAAGAATAACATTTTAGCAGTAATCCTATGAATTCTAAGTTCTATCGCTTCATCt                                             >  1:914936/1‑141 (MQ=255)

                                                                                                  cttcttTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCTAAGTTCTATCGCTTCATCTa                                            >  1:303774/1‑141 (MQ=255)

                                                                                                        tCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCTAAGTTCTATCGCTTCATCTAACGTTg                                      >  4:417880/1‑141 (MQ=255)

                                                                                                            ccACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCTAAGTTCTATCGCTTCATCTAACGTTGCTAc                                  >  1:578415/1‑141 (MQ=255)

                                                                                                            ccACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCTAAGTTCTATCGCTTCATCTAACGTTGCTAc                                  <  1:527861/141‑1 (MQ=255)

                                                                                                             cACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCTAAGTTCTATCGCTTCATCTAACGTTGCTACa                                 <  1:814613/141‑1 (MQ=255)

                                                                                                               cTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCTAAGTTCTATCGCTTCATCTAACGTTGCTACAGc                               <  3:223020/141‑1 (MQ=255)

                                                                                                               cTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACGACAATTTTAGCAGTAATCCCATGCATTCTAAGTTCTATCGCTTCATCTAACGTTGCTACAGc                               >  2:844771/1‑141 (MQ=255)

                                                                                                                tGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCTAAGTTCTATCGCTTCATCTAACGTTGCTACAGCa                              >  1:678771/1‑141 (MQ=255)

                                                                                                                      gACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCTAAGTTCTATCGCTTCATCTAACGTTGCTACAGCAAAAAAt                        <  4:274168/141‑1 (MQ=255)

                                                                                                                      gACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCTAAGTTCTATCGCTTCATCTAACGTTGCTACAGCAAAAAAt                        >  1:1038159/1‑141 (MQ=255)

                                                                                                                      gACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCTAAGTTCTATAGCTTCATCTAACGTTGCTACAGCAAAAAAt                        >  1:48114/1‑141 (MQ=255)

                                                                                                                        cGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCTAAGTTCTATCGCTTCATCTAACGTTGCTACAGCAAAAAAtgt                      >  1:365570/1‑141 (MQ=255)

                                                                                                                           aaCCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCTAAGTTCTATCGCTTCATCTAACGTTGCTACAGCAAAAAATGTGgc                   >  3:237113/1‑141 (MQ=255)

                                                                                                                            ttcGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCTAAGTTCTATCTCTTCATCTAACGTTGCTACAGCAAAAAATGTGgcg                  <  2:58233/139‑1 (MQ=255)

                                                                                                                            aCCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCTAAGTTCTATCGCTTCATCTAACGTTGCTACAGCAAAAAATGTGgcg                  >  3:976/1‑141 (MQ=255)

                                                                                                                              cGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCTAAGTTCTATCGCTTCATCTAACGTTGCTACAGCAAAAAATGTGGCGcc                >  2:1047141/1‑141 (MQ=255)

                                                                                                                                ttAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCTAAGTTCTATCGCTTCATCTAACGTTGCTACAGCAAAAAATGTGGCGCCAt              >  3:57640/1‑141 (MQ=255)

                                                                                                                                ttAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCTAAGTTCTATCGCTTCATCTAACGTTGCTACAGCAAAAAATGTGGCGCCAt              >  2:443666/1‑141 (MQ=255)

                                                                                                                                  ggggCAACTCGGTGTTGTATCGCTTTATGGATGTCTGTTGCTGGTAGCACACCTAAGACTAGAATTTTAGCAGTAAGCACATCCATTCTAAGGTCTGTCGCTTCATCTACCGTGGCGACAGCAAAAAATGTGGCGCCAttt            <  2:707128/139‑1 (MQ=255)

                                                                                                                                  aaGGCAACTCTGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCTAAGTTCTATCGCTTCATCTAACGTTGCTACAGCAAAAAATGTGGCGCCAttt            <  2:1022326/141‑1 (MQ=255)

                                                                                                                                     gCAACTCTGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCTAAGTTCTATCGCTTCATCTAACGTTGCTACAGCAAAAAATGTGGCGCCATTTTcc         <  1:524448/141‑1 (MQ=255)

                                                                                                                                     gCAACTCGGTGTTTTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCTAAGTTCTATCGCTTCATCTAACGTTGCTACAGCAAAAAATGTGGCGCCATTTTcc         <  3:131688/141‑1 (MQ=255)

                                                                                                                                         cTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCTAAGTTCTATCGCTTCATCTAACGTTGCTACAGCAAAAAATGTGGCGCCATTTTCCATTa     <  2:533294/141‑1 (MQ=255)

                                                                                                                                         cTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCTAAGTTCTATCGCTTCATCTAACGTTGCTACAGCAAAAAATGTGGCGCCATTTTCCATTa     >  1:931449/1‑141 (MQ=255)

                                                                                                                                            ggTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCTAAGTTCTATCGCTTCATCTAACGTTGCTACAGCAAAAAATGTGGCGCCATTTTCCATTAAAt  >  4:232315/1‑141 (MQ=255)

CGTTTTAGTATCTTTAATACCTAAACGTCCCATTCCTGTATCTAATTTAATGTGCAACCATAACTTTTTCTCTTGCTCACCAGAAATGTTTTTAATTGCTTCTTTCAACCACTGTTTAGACGGAACCGTTAAGGCAACTCGGTGTTGTATCGCTTTATCAATATCTTTAGCTGGTAACACACCTAAGACTAAAATTTTAGCAGTAATCCCATGCATTCTAAGTTCTATCGCTTCATCTAACGTTGCTACAGCAAAAAATGTGGCGCCATTTTCCATTAAAT  >  USA300TCH1516_ALE/2189737‑2190017

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`