| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 2,326,699 | A→T | 6.4% | I412K (ATA→AAA) | lacG ← | 6‑phospho‑beta‑galactosidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 2,326,699 | 0 | A | T | 6.4% | 404.6 / 14.9 | 126 | I412K (ATA→AAA) | lacG | 6‑phospho‑beta‑galactosidase |
| Reads supporting (aligned to +/- strand): ref base A (65/52); new base T (6/2); total (72/54) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.64e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.31e-01 | |||||||||||
Only 100 of 130 total aligned reads displayed.
ATAAGCACTTTTCTTAGGATAACGTTCTTGAGTCTCAAAATCGACATAGAATAAACCATATCGTTTTTCATAACCATTTGACCAAGAGAACACATCCATTAATGACCAAATAAAGTAACCTTTAACATTTGCACCATCTATAATAGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATGATAGTTAGG > USA300TCH1516_ALE/2326560‑2326838 | aTAAGCACTTTTCTTAGGATAACGTTCTTGAGTCTCAAAATCGACATAGAATAAACCATATCGTTTTTCATAACCATTTGACCAAGAGAACACATCCATTAATGACCAAATAAAGTAACCTTTAACATTTGCACCATCtat > 3:220379/1‑141 (MQ=255)aTAAGCACTTTTCTTAGGATAACGTTCTTGAGTCTCAAAATCGACATAGAATAAACCATATCGTTTTTCATAACCATTTGACCAAGAGAACACATCCATTAATGACCAAATAAAGTAACCTTTAACATTTGCACCATCtat > 1:364426/1‑141 (MQ=255) cACTTTTCTTAGGATAACGTTCTTGAGTCTCAAAATCGACATAGAATAAACCATATCGTTTTTCATAACCATTTGACCAAGAGAACACATCCATTAATGACCAAATAAAGTAACCTTTAACATTTGCACCATCTATAATAg > 2:986273/1‑141 (MQ=255) cACTTTTCTTAGGATAACGTTCTTGAGTCTCAAAATCGACATAGAATAAACCATATCGTTTTTCATAACCATTTGACCAAGAGAACACATCCATTAATGACCAAATAAAGTAACCTTTAACATTTGCACCATCTATAATAg < 4:318472/141‑1 (MQ=255) cTTTTCTTAGGATAACGTTCTTGAGTCTCAAAATCGACATAGAATAAACCATATCGTTTTTCTTTTTTTTTTGACCAAGAGAACACATCCATTAATGACCAAATAAAGTAACCTTTAACATTTGCACCATCTCTAATAGCa > 2:245027/1‑141 (MQ=255) cTTTTCTTAGGATAACGTTCTTGAGTCTCAAAATCGACATAGAATAAACCATATCGTTTTTCATAACCATTTGACCAAGAGAACACATCCATTAATGACCAAATAAAGTAACCTTTAACATTTGCACCATCTTTAATAGCa > 1:363884/1‑141 (MQ=255) ttttCTTAGGATAACGTTCTTGAGTCTCAAAATCGACATAGAATAAACCATATCGTTTTTCATAACCATTTGACCAAGAGAACACATCCATTAATGACCAAATAAAGTAACCTTTAACATTTGCACCATCTATAATAGCAt > 4:132464/1‑141 (MQ=255) tttCTTAGGATAACGTTCTTGAGTCTCAAAATCGACATAGAATAAACCATATCGTTTTTCATAACCATTTGACCAAGAGAACACATCCATTAATGACCAAATAAAGTAACCTTTAACATTTGCACCATCTATAATAGCATc > 1:700055/1‑141 (MQ=255) tCTTAGGATAACGTTCTTGAGTCTCAAAATCGACATAGAATAAACCATATCGTTTTTTTTTTTTTTTTGACCAAGAGAACACATCCATTAATGACCAAATAAAGTAACCTTTAACATTTGCACCATCTATAATAGCATCTg > 2:262578/1‑141 (MQ=255) cTTAGGATAACGTTCTTGAGTCTCAAAATCGACATAGAATAAACCATATCGTTTTTCATAACCATTTGACCAAGAGAACACATCCATTAATGACCAAATAAAGTAACCTTTAACATTTGCACCATCTATAATAGCATCTGc < 2:835791/141‑1 (MQ=255) cTTAGGATAACGTTCTTGAGTCTCAAAATCGACATAGAATAAACCATATCGTTTTTCATAACCATTTGACCAAGAGAACACATCCATTAATGACCAAATAAAGTAACCTTTAACATTTGCACCATCTATAATAGCATCTGc < 1:331632/141‑1 (MQ=255) aGGATAAGGTTCTTGAGTCTCAAAATCGACATAGAATAAACCATATCGTTTTTCATAACCATTTGACCAAGAGAACACATCCATTAATGACCAAATAAAGTAACCTTTAACATTTGCACCATCTATAATAGCATCTGCAAt > 1:859976/1‑141 (MQ=255) ggATAACGTTCTTGAGTCTCAAAATCGACATAGAATAAACCATATCGTTTTTCATAACCATTTGACCAAGAGAACACATCCATTAATGACCAAATAAAGTAACCTTTAACATTTGCACCATCTATAATAGCATCTGCAATa > 4:164220/1‑141 (MQ=255) gATAACGTTCTTGAGTCTCAAAATCGACATAGAATAAACCATATCGTTTTTTTTTTTTTTTTTACCAAGAGAACACATCCATTAATTACCAAATAAAGTAACCTTTAACATTTGCACCATCCATAATACCATCTGCAATaa > 2:619067/1‑141 (MQ=255) gATAACGTTCTTGAGTCTCAAAATCGACATAGAATAAACCATATCGTTTTTCATAACCATTTGACCAAGAGAACACATCCATTAATGACCAAATAAAGTAACCTTTAACATTTGCACCATCTTTAATAGCATCTGCAATaa > 1:141914/1‑141 (MQ=255) gATAACGTTCTTGAGTCTCAAAATCGACATAGAATAAACCATATCGTTTTTCATAACCATTTGACCAAGAGAACACATCCATTAATGACCAAATAAAGTAACCTTTAACATTTGCACCATCTATAATAGCATCTGCAATaa < 2:1022071/141‑1 (MQ=255) aaCGTTCTTGAGTCTCAAAATCGACATAGAATAAACCATATCGTTTTTCATAACCATTTGACCAAGAGAACACATCCATTAATGACCAAATAAAGTAACCTTTAACATTTGCACCATCTATAATAGCATCTGCAATAACGt < 3:215984/141‑1 (MQ=255) aaCGTTCTTGAGTCTCAAAATCGACATAGAATAAACCATATCGTTTTTCATAACCATTTGACCAAGAGAACACATCCATTAATGACCAAATAAAGTAACCTTTAACATTTGCACCATCTATAATAGCATCTGCAATAACGt < 4:108170/141‑1 (MQ=255) aCGTTCTTGAGTCTCAAAATCGACATAGAATAAACCATATCGTTTTTCATAACCATTTGACCAAGAGAACACATCCATTAATGACCAAATAAAGTAACCTTTAACATTTGCACCATCTATAATAGCATCTGCAATAACGtt < 2:156089/141‑1 (MQ=255) gTTCTTGAGTCTCAAAATCGACATAGAATAAACCATATCGTTTTTCATAACCATTTGACCAAGAGAACACATCCATTAATGACCAAATAAAGTAACCTTTAACATTTGCACCATCTATAATAGCATCTGCAATAACGTTCa < 2:176537/141‑1 (MQ=255) tCTTGAGTCTCAAAATCGACATAGAATAAACCATATCGTTTTTCATAACCATTTGACCAAGAGAACACATCCATTAATGACCAAATAAAGTAACCTTTAACATTTGCACCATCTATAATAGCATCTGCAATAACGTTCaaa < 1:963530/141‑1 (MQ=255) tCTTGAGTCTCAAAATCGACATAGAATAAACCATATCGTTTTTCATAACCATTTGACCAAGAGAACACATCCATTAATGACCAAATAAAGTAACCTTTAACATTTGCACCATCTATAATAGCATCTGCAATAACGTTCaaa < 1:1042660/141‑1 (MQ=255) tCTTGAGTCTCAAAATCGACATAGAATAAACCATATCGTTTTTCATAACCATTTGACCAAGAGAACACATCCATTAATGACCAAATAAAGTAACCTTTAACATTTGCACCATCTATAATAGCATCTGCAATAACGTTCaaa < 1:819316/141‑1 (MQ=255) cTTGAGTCTCAAAATCGACATAGAATAAACCATATCGTTTTTCATAACCATTTGACCAAGAGAACACATCCATTAATGACCAAATAAAGTAACCTTTAACATTTGCACCATCTATAATAGCATCTGCAATAACGTTCAAAt > 1:854261/1‑141 (MQ=255) cTTGAGTCTCAAAATCGACATAGAATAAACCATATCGTTTTTCATAACCATTTGACCAAGAGAACACATCCATTAATGACCAAATAAAGTAACCTTTAACATTTGCACCATCTATAATAGCATCTGCAATAACGTTCAAAt > 2:91665/1‑141 (MQ=255) cTTGAGTCTCAAAATCGACATAGAATAAACCATATCGTTTTTCATAACCATTTGACCAAGAGAACACATCCATTAATGACCAAATAAAGTAACCTTTAACATTTGCACCATCTATAATAGCATCTGCAATAACGTTCAAAt > 2:14158/1‑141 (MQ=255) cTTGAGTCTCAAAATCGACATAGAATAAACCATATCGTTTTTCATAACCATTTGACCAAGAGAACACATCCATTAATGACCAAATAAAGTAACCTTTAACATTTGCACCATCTATAATAGCATCTGCAATAACGTTCAAAt > 3:212191/1‑141 (MQ=255) cTTGAGTCTCAAAATCGACATAGAATAAACCATATCGTTTTTCATAACCATTTGACCAAGAGAACACATCCATTAATGACCAAATAAAGTAACCTTTAACATTTGCACCATCTATAATAGCATCTGCAATAACGTTCAAAt > 1:69208/1‑141 (MQ=255) tGAGTCTCAAAATCGACATAGAATAAACCATATCGTTTTTCATAACCATTTGACCAAGAGAACACATCCATTAATGACCAAATAAAGTAACCTCTAACATTTGCACCATCTATAATAGCATCTGCAATAACGTTCAAAtgt < 2:881325/141‑1 (MQ=255) tGAGTCTCAAAATCGACAGAGAATAAACCATATCGTTTTTCATAACCATTTGACCAAGAGAACACATCCATTAATGACCAAATAAAGTAACCTTTAACATTTCCACCATCTATAATAGCATCTGCAATAACGTTCAAAtgt < 1:158464/141‑1 (MQ=255) gAGTCTCAAAATCGACATAGAATAAACCATATCGTTTTTCATAACCCTTTGACCAAGAGAACACATCCATTAATGACCAAATAAAGTAACCTTTAACATTTGCCCCATCTATAATAGCATCTGCAATAACGTTCAAAtgtt > 3:374495/1‑141 (MQ=255) aGTCTCAAAATCGACATAGAATAAACCATATCGTTTTTCATAACCATTTGACCAAGAGAACACATCCATTAATGACCAAATAAAGTAACCTTTAACATTTGCACCATCTATAATAGCATCTGCAATAACGTTCAAAtgttg < 1:623607/141‑1 (MQ=255) gTCTCAAAATCGACATAGAATAAACCATATCGTTTTTCATAACCATTTGACCAAGAGAACACATCCATTAATGACCAAATAAAGTAACCTTTAACATTTGCACCATCTATAATAGCATCTGCAATAACGTTCAAAtgttgt < 2:213059/141‑1 (MQ=255) tctcAAAATCGACATAGAATAAACCATATCGTTTTTCATAACCATTTGACCAAGAGAACACATCCATTAATGACCAAATAAAGTAACCTTTAACATTTGCACCATCTATAATAGCATCTGCAATAACGTTCAAATGTTGTc < 3:316457/141‑1 (MQ=255) cGACATAGAATAAACCATATCGTTTTTCATAACCATTTGACCAAGAGAACACATCCATTAATGACCAAATAAAGTAACCTTTAACATTTGCACCATCTTTAATAGCATCTGCAATAACGTTCAAATGTTGTCTTACATAAt > 2:44337/1‑141 (MQ=255) cGACATAGAATAAACCATATCGTTTTTCATAACCATTTGACCAAGAGAACACATCCATTAATGACCAAATAAAGTAACCTTTAACATTTGCACCATCTATAATAGCATCTGCAATAACGTTCAAATGTTGTCTTACATAAt > 1:320786/1‑141 (MQ=255) cGACATAGAATAAACCATATCGTTTTTCATAACCATTTGACCAAGAGAACACATCCATTAATGACCAAATAAAGTAACCTTTAACATTTGAACCATCTATAATAGCATCTGCAATAACGTTCAAATGTTGTCTTACATAAt > 4:155031/1‑141 (MQ=255) cATAGAATAAACCATATCGTTTTTCATAACCATTTGACCAAGAGAACACATCCATTAATGACCAAATAAAGTAACCTTTAACATTTGCACCATCTATAATAGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCaa > 2:119150/1‑141 (MQ=255) aTAGAATAAACCATATCGTTTTTCATAACCATTTGACCAAGAGAACACATCCATTAATGACCAAATAAAGTAACCTTTAACATTTGCACCATCTATAATAGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAAt < 1:523916/141‑1 (MQ=255) aTAGAATAAACCATATCGTTTTTCATAACCATTTGACCAAGAGAACACATCCATTAATGACCAAATAAAGTAACCTTTAACATTTGCACCATCTATAATAGCATCTGCAATAACGTTCAAATGTTGTCTTACAAAATCAAt > 1:879077/1‑141 (MQ=255) tAGAATAAACCATATCGTTTTTCATAACCATTTGACCAAGAGAACACATCCATTAATGACCAAATAAAGTAACCTTTAACATTTGCACCATCTATAATAGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATa > 1:352049/1‑141 (MQ=255) gAATAAACCATATCGTTTTTCATAACCATTTGACCAAGAGAACACATCCATTAATGACCAAATAAAGTAACCTTTAACATTTGCACCATCTTTAATAGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACg < 4:148789/141‑1 (MQ=255) aaTAAACCATATCGTTTTTCATAACCATTTGACCAAGAGAACACATCCATTAATGACCAAATAAAGTAACCTTTAACATTTGCACCATCTATAATAGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGt > 1:61566/1‑141 (MQ=255) aaaCCATATCGTTTTTCATAACCATTTGACCAAGAGAACACATCCATTAATAACCAAATAAAGTAACCTTTAACATTTGCACCATCTATAATAGCATCTGCAATCACGTTCAAATGTTGTCTTACATAATCAATGCGTGca > 4:361740/1‑141 (MQ=255) aaCCATATCGTTTTTCATAACCATCTGACCAAGAGAACACATCCATTAATGACCAAATAAAGTAACCTTTAACATTTGCACCATCTATAATAGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGcat > 1:382892/1‑141 (MQ=255) aCCATATCGTTTTTCATAACCATTTGACCAAGAGAACACATCCATTAATGACCAAATAAAGTAACCTTTAACATTTGCACCATCTATAATAGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGcatc > 1:140495/1‑141 (MQ=255) ccATATCGTTTTTCATAACCATTTGACCAAGAGAACACATCCATTAATGACCAAATAAAGTAACCTTTAACATTTGCACCATCTTTAATAGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGcatca > 1:797425/1‑141 (MQ=255) ccATATCGTTTTTCATAACCATTTGACCAAGAGAACACATCCATTAATGACCAAATAAAGTAACCTTTAACATTTGCACCATCTATAATAGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGcatca > 1:333706/1‑141 (MQ=255) cATATCGTTTTTCATAACCATTTGACCAAGAGAACACATCCATTAATGACCAAATAAAGTAACCTTTAACATTTGCACCATCTATAATAGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGcatcat > 4:252116/1‑141 (MQ=255) tCGTTTTTCATAACCATTTGACCAAGAGAACACATCCATTAATGACCAAATAAAGTAACCTTTAACATTTGCACCATCTATAATAGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGCATCATCATg > 4:447978/1‑141 (MQ=255) tttttCATAGCCATTTGACCAAGAGAACACATCCATTAATGACCAAATAAAGTAACCTTTAACATTTGCACCATCTATAATAGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAAc < 2:303859/141‑1 (MQ=255) tttttCATAACCCTTTCACCAAGAGAACACATCCATTAATGACCAAATAAAGTAACCTTTAACATTTGCACCATCTATAATAGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAAc < 3:24158/141‑1 (MQ=255) tttttCATAACCATTTGACCAAGAGAACACATCCATTAATGACCAAATAAAGTAACCTTTAACATTTGCACCATCTATAATAGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAAc < 3:117915/141‑1 (MQ=255) tttttCATAACCATTTGACCAAGAGAACACATCCATTAATGACCAAATAAAGTAACCTTTAACATTTGCACCATCTATAATAGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAAc < 2:724450/141‑1 (MQ=255) tttttCATAACCATTTGACCAAGAGAACACATCCATTAATGACCAAATAAAGTAACCTTTAACATTTGCACCATCTATAATAGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAAc > 4:454378/1‑141 (MQ=255) tttttCATAACCATTTGACCAAGAGAACACATCCATTAATGACCAAATAAAGTAACCTTTAACATTTGCACCATCTATAATAGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAAc < 1:153880/141‑1 (MQ=255) tttttCATAACCATTTGACCAAGAGAACACATCCATTAATGACCAAATAAAGTAACCTTTAACATTTGCACCATCTATAATAGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAAc > 2:1035907/1‑141 (MQ=255) ttCATAACCATTTGACCAAGAGAACACATCCATTAATGACCAAATAAAGTAACCTTTAACATTTGCACCATCTATAATAGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAACTGt < 2:692936/141‑1 (MQ=255) tCATAACCATTTGACCAAGAGAACACATCCATTAATGACCAAATAAAGTAACCTTTAACATTTGCACCATCTATAATAGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAACTGtt < 1:115447/141‑1 (MQ=255) tCATAACCATTTGACCAAGAGAACACATCCATTAATGACCAAATAAAGTAACCTTTAACATTTGCACCATCTATAATAGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAACTGtt < 2:836197/141‑1 (MQ=255) cATAACCATTTGACCAAGAGAACACATCCATTAATGACCAAATAAAGTAACCTTTAACATTTGCACCATCTATAATAGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAACTGttt < 1:687626/141‑1 (MQ=255) tAACCATTTGACCAAGAGAACACATCCATTAATGACCAAATAAAGTAACCTTTAACATTTGCACCATCTTTAATAGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAACTGttttt > 2:934814/1‑141 (MQ=255) aaCCATTTGACCAAGAGAACACATCCATTAATGACCAAATAAAGTATCCTTTAACATTTGCACCATCTATAATAGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGGTCATCATCATGAACTGtttttt > 3:380096/1‑141 (MQ=255) aaCCATTTGACCAAGAGAACACATCCATTAATGACCAAATAAAGTAACCTTTAACATTTGCACCATCTATAATAGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAACTGtttttt > 2:301641/1‑141 (MQ=255) aCCATTTGACCAAGAGAACACATCCATTAATGACCAAATAAAGTAACCTTTAACATTTGCACCATCTTTAATAGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAACTGTTTTTTc > 3:335409/1‑141 (MQ=255) tGACCAAGAGAACACATCCATTAATGACCAAATAAAGTAACCTTTAACATTTGCACCATCTATAATAGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAACTGTTTTTTCAGATTc < 4:453308/141‑1 (MQ=255) gACCAAGAGAACACATCCATTAATGACCAAATAAAGTAACCTTTAACATTTGCACCATCTATAATAGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAACTGTTTTTTCAGATTCa > 2:683182/1‑141 (MQ=255) aCCAAGAGAACACATCCATTAATGACCAAATAAAGTAACCTTTAACATTTGCACCATCTATAATAGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAACTGTTTTTTCAGATTCaa < 3:197812/141‑1 (MQ=255) aCCAAGAGAACACATCCATTAATGACCAAATAAAGTAACCTTTAACATTTGCACCATCTATAATAGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAACTGTTTTTTCAGATTCaa < 1:633750/141‑1 (MQ=255) aCCAAGAGAACACATCCATTAATGACCAAATAAAGTAACCTTTAACATTTGCACCATCTATAATAGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAACTGTTTTTTCACATTCaa > 2:710049/1‑141 (MQ=255) aaGAGAACACATCCATTAATGACCAAATAAAGTAACCTTTAACATTTGCACCATCTATAATAGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAACTGTTTTTTCAGATTCAATaa > 2:1056494/1‑141 (MQ=255) aaGAGAACACATCCATTAATGACCAAATAAAGTAACCTTTAACATTTGCACCATCTATAATAGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAACTGTTTTTTCAGATTCAATaa > 1:521563/1‑141 (MQ=255) gagaACACATCCATTAATGACCAAATAAAGTAACCTTTAACATTTGCACCATCTATAATAGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAACTGTTTTTTCAGATTCAATAAAt > 1:1052409/1‑141 (MQ=255) cacaTCCATTAATGACCAAATAAAGTAACCTTTAACATTTGCACCATCTATAATAGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATc > 2:828940/1‑141 (MQ=255) acaTCCATTAATGACCAAATAAAGTAACCTTTAACATTTGCACCATCTATAATAGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCt > 1:515213/1‑141 (MQ=255) tGACCAAATAAAGTAACCTTTAACATTTGCACCATCTATAATAGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTaa > 1:600551/1‑141 (MQ=255) tGACCAAATAAAGTAACCTTTAACATTTGCACCATCTATAATAGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTaa > 3:84256/1‑141 (MQ=255) aCCAAATAAAGTAACCTTTAACATTTGCACCATCTATAATAGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAAc > 4:379865/1‑141 (MQ=255) aCCAAATAAAGTAACCTTTAACATTTGCACCATCTATAATAGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAAc < 1:662488/141‑1 (MQ=255) aCCAAATAAAGTAACCTTTAACATTTGCACCATCTATAATAGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAAc < 2:655557/141‑1 (MQ=255) aCCAAATAAAGTAACCTTTAACATTTGCACCATCTATAATAGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAAc < 3:140742/141‑1 (MQ=255) aTAAAGTAACCTTTAACATTTGCACCATCTATAATAGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCAttt > 3:314206/1‑141 (MQ=255) aaGTAACCTTTAACATTTGCACCATCTATAATAGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCa < 1:136253/141‑1 (MQ=255) gTAACCTTTAACATTTGCACCATCTATAATAGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGt < 3:392441/141‑1 (MQ=255) gTAACCTTTAACATTTGCACCATCTATAATAGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGt < 2:669925/141‑1 (MQ=255) tAACCTTTAACATTTGCACCATCTATAATAGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTg < 1:940490/141‑1 (MQ=255) aaCCTTTAACATTTGCACCATCTATAATAGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGa > 2:406745/1‑141 (MQ=255) aCCTTTAACATTTGCACCATCTATAATAGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGat < 2:534240/141‑1 (MQ=255) aCCTTTAACATTTGCACCATCTATAATAGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGat > 3:170658/1‑141 (MQ=255) cTTTAACATTTGCACCATCTATAATAGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGatat < 2:631540/141‑1 (MQ=255) cTTTAACATTTGCACCATCTATAATAGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGatat < 2:439753/141‑1 (MQ=255) tttGCACCATCTATAATAGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAACTGTTTTTTCATATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTa > 2:366152/1‑141 (MQ=255) tttGCACCATCTATAATAGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTa > 3:305118/1‑141 (MQ=255) tGCACCATCTATAATAGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATg > 1:190020/1‑141 (MQ=255) tGCACCATCTATAATAGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATg > 2:978308/1‑141 (MQ=255) gCACCATCTATAATAGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATGa > 1:978306/1‑141 (MQ=255) cATCTATAATAGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATGATAGt < 1:1007939/141‑1 (MQ=255) tCTATAATAGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAAGCATTTTCAGTGATATAAATCTTATgatagata > 2:626412/1‑141 (MQ=255) cTATAATAGCATCTGCAATAACGTTCAAATGTTGTCTAACATAATCAATACGTGCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATGATAGTTAg > 1:785493/1‑141 (MQ=255) tataATAGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATGATAGTTAgg < 2:71574/141‑1 (MQ=255) | ATAAGCACTTTTCTTAGGATAACGTTCTTGAGTCTCAAAATCGACATAGAATAAACCATATCGTTTTTCATAACCATTTGACCAAGAGAACACATCCATTAATGACCAAATAAAGTAACCTTTAACATTTGCACCATCTATAATAGCATCTGCAATAACGTTCAAATGTTGTCTTACATAATCAATACGTGCATCATCATGAACTGTTTTTTCAGATTCAATAAATTCATCTTTATATCCTAAACCATTTTCAGTGATATAAATCTTATGATAGTTAGG > USA300TCH1516_ALE/2326560‑2326838 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |