Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 2,330,500 A→G 5.5% F161F (TTT→TTC lacD ← Tagatose 1,6‑diphosphate aldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,330,5000AG 5.5% 491.1 / 11.8 160F161F (TTT→TTClacDTagatose 1,6‑diphosphate aldolase
Reads supporting (aligned to +/- strand):  ref base A (80/71);  new base G (7/2);  total (87/73)
Fisher's exact test for biased strand distribution p-value = 1.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.78e-01

Only 100 of 167 total aligned reads displayed.

ACTTCAACTTTCAATACATCAACGTTAAAGCGTGGTTCTGAGAATAATTTCATTGCTTCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAACATCATAGTAAAGTAAGAATTTAACAGCATTTGCGCCTTGTTCTTTTAAACG  >  USA300TCH1516_ALE/2330360‑2330640
                                                                                                                                            |                                                                                                                                            
aCTTCAACTTTCAATACATCAACGTTAAAGCGTGGTTCTGAGAATAATTTCATTGCTTCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTaaa                                                                                                                                              >  1:806180/1‑141 (MQ=255)
  ttCAACTTTCAATACATCAACGTTAAAGCGTGGTTCTGAGAATAATTTCATTGCTTCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTAAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTaaaaa                                                                                                                                            <  1:866577/141‑1 (MQ=255)
     atctctCAATACATCACCGTTAAAGCGTGGTTCTGAAAATAATTTCATTGCTTCGTTAACCTTTCTAGGATTAACTTTTTCGAATTCAACAATACCGTTATCATGAATGTTGTCATCGTATGTTAATACTTCTAAAATGaa                                                                                                                                         <  4:446936/136‑1 (MQ=255)
     aaCTTTCAATACATCAACGTTAAAGCGTGGTTCTGAGAATAATTTCATTGCTTCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGaa                                                                                                                                         >  1:831276/1‑141 (MQ=255)
     aaCTTTCAATACATCAACGTTAAAGCGTGGTTCTGAGAATAATTTCATTGCTTCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGaa                                                                                                                                         >  2:686621/1‑141 (MQ=255)
       cTTTCAATACATCAACGTTAAAGCGTGGTTCTGAGAATAATTTCATTGCTTCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATg                                                                                                                                       >  2:137953/1‑141 (MQ=255)
        tttCAATACATCAACGTTAAAGCGTGGTTCTGAGAATAATTTCATTGCTTCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATgg                                                                                                                                      <  1:1028256/141‑1 (MQ=255)
        tttCAATACATCAACGTTAAAGCGTGGTTCTGAGAATAATTTCATTGCTTCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATgg                                                                                                                                      <  1:152753/141‑1 (MQ=255)
        tttCAATACATCAACGTTAAAGCGTGGTTCTGAGAATAATTTCATTGCTTCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATgg                                                                                                                                      <  3:174878/141‑1 (MQ=255)
         ttCAATACATCAACGTTAAAGCGTGGTTCTGAGAATAATTTCATTGCTTCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGa                                                                                                                                     <  1:540535/141‑1 (MQ=255)
           cAATACATCAACGTTAAAGCGTGGTTCTGAGAATAATTTCATTGCTTCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAat                                                                                                                                   >  4:81627/1‑141 (MQ=255)
           cAATACATCAACGTTAAAGCGTGGTTCTGAGAATAATTTCATTGCTTCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAat                                                                                                                                   >  1:473470/1‑141 (MQ=255)
            aaTACATCAACGTTAAAGCGTGGTTCTGAGAATAATTTCATTGCTTCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAata                                                                                                                                  >  2:398908/1‑141 (MQ=255)
             aTACATCAACGTTAAAGCGTGGTTCTGAGAATAATTTCATTGCTTCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAatat                                                                                                                                 <  4:292016/141‑1 (MQ=255)
               aCATCAACGTTAAAGCGTGGTTCTGAGAATAATTTCATTGCTTCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATAtct                                                                                                                               >  4:115361/1‑141 (MQ=255)
               aCATCAACGTTAAAGCGTGGTTCTGAGAATAATTTCATTGCTTCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATAtct                                                                                                                               >  2:185065/1‑141 (MQ=255)
                   cAACGTTAAAGCGTGGTTCTGAGAATAATTTCATTGCTTCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTg                                                                                                                           <  3:298809/141‑1 (MQ=255)
                   cAACGTTAAAGCGTGGTTCTGAGAATAATTTCATTGCTTCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTg                                                                                                                           >  1:824757/1‑141 (MQ=255)
                       gTTAAAGCGTGGTTCTGAGAATAATTTCATTGCTTCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAac                                                                                                                       <  2:887081/141‑1 (MQ=255)
                        ttAAAGCGTGGTTCTGAGAATAATTTCATTGCTTCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAaca                                                                                                                      >  2:989356/1‑141 (MQ=255)
                          aaaGCGTGGTTCTGAGAATAATTTCATTGCTTCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAAcaca                                                                                                                    <  2:595427/141‑1 (MQ=255)
                            aGCGTGGTTCTGAGAATAATTTCATTGCTTCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACAtt                                                                                                                  >  1:662952/1‑141 (MQ=255)
                             gCGTGGTTCTGAGAATAATTTCATTGCTTCGTTAAGCTTTCTCGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTc                                                                                                                 >  1:934283/1‑141 (MQ=255)
                             gCGTGGTTCTGAGAATAATTTCATTGCTTCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTc                                                                                                                 >  1:734322/1‑141 (MQ=255)
                              cGTGTNTCTGAGAATAATTTCATTGCTTCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCt                                                                                                                <  1:367980/141‑1 (MQ=255)
                              cGTGGTTCTGAGAATAATTTCATTGCTTCGTTAACCTTTCTAGGCTTAACTTTTTTTTTTTTTTCTCTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCt                                                                                                                >  2:150669/1‑141 (MQ=255)
                              cGTGGTTCTGAGAATAATTTCATTGCTTCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCt                                                                                                                >  2:526954/1‑141 (MQ=255)
                               gTGGTTCTGAGAATAATTTCATTGCTTCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAGAAGAAAGGAATGTCTTCTGCAACACATTCTg                                                                                                               >  1:271272/1‑141 (MQ=255)
                                tGGTTCTGAGAATAATTTCATTGCTTCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGa                                                                                                              >  2:970694/1‑141 (MQ=255)
                                  gTTCTGAGAATACTTTCATTGCTTCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAAc                                                                                                            <  2:270607/141‑1 (MQ=255)
                                          aataatTTCATTGCTTCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCtt                                                                                                    <  1:470575/141‑1 (MQ=255)
                                          aataatTTCATTGCTTCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCtt                                                                                                    <  2:421489/141‑1 (MQ=255)
                                          aataatTTCATTGCTTCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCtt                                                                                                    <  3:418311/141‑1 (MQ=255)
                                              atTTCATTGCTTCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCaa                                                                                                >  1:169423/1‑141 (MQ=255)
                                                  cATTGCTTCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTa                                                                                            <  2:760263/141‑1 (MQ=255)
                                                  cATTGCTTCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTa                                                                                            <  1:712056/141‑1 (MQ=255)
                                                     tGCTTCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGc                                                                                         <  2:868149/141‑1 (MQ=255)
                                                     tGCTTCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGc                                                                                         <  1:91547/141‑1 (MQ=255)
                                                     tGCTTCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGc                                                                                         <  1:566129/141‑1 (MQ=255)
                                                      gCTTCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTTCAACACATTCTGAACCGATTCTTTCAATGTATGct                                                                                        >  2:1061960/1‑141 (MQ=255)
                                                       cTTCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATNTATGctt                                                                                       >  2:814271/1‑141 (MQ=255)
                                                       cTTCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGctt                                                                                       <  3:188962/141‑1 (MQ=255)
                                                        ttCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGcttt                                                                                      >  1:301189/1‑141 (MQ=255)
                                                         tCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACACGTTCTTTCAATGTATGctttc                                                                                     >  3:3644/1‑141 (MQ=255)
                                                           gTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGctttctt                                                                                   >  2:847239/1‑141 (MQ=255)
                                                             tAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCtttt                                                                                 >  1:1021987/1‑141 (MQ=255)
                                                              aaCCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCGGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTg                                                                                <  2:1054248/141‑1 (MQ=255)
                                                               aCCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGa                                                                               >  2:908189/1‑141 (MQ=255)
                                                                ccTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGaa                                                                              >  1:247075/1‑141 (MQ=255)
                                                                 cTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTa                                                                                            >  2:1023629/1‑126 (MQ=255)
                                                                 cTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAAt                                                                             <  1:264605/141‑1 (MQ=255)
                                                                     cTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTa                                                                         <  2:243007/141‑1 (MQ=255)
                                                                             aaCTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTCAAACGAATGGAATTTCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATttcttc                                                                 <  1:214676/141‑1 (MQ=255)
                                                                              aCTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAGAAGAAAGGAATGTCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCa                                                                >  1:31098/1‑141 (MQ=255)
                                                                              aCTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAGAAGAAAGGAATGTCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCa                                                                >  1:913347/1‑141 (MQ=255)
                                                                               cTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAg                                                               <  3:418671/141‑1 (MQ=255)
                                                                               cTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATACTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAg                                                               >  3:123367/1‑141 (MQ=255)
                                                                                ttttGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGc                                                              <  4:271157/141‑1 (MQ=255)
                                                                                    gCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGcatca                                                          >  2:549642/1‑141 (MQ=255)
                                                                                    gCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGcatca                                                          >  2:549999/1‑141 (MQ=255)
                                                                                    gCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGcatca                                                          >  2:809997/1‑141 (MQ=255)
                                                                                      gAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATAATTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCatcatc                                                        <  2:350912/141‑1 (MQ=255)
                                                                                         ttCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAAc                                                     <  2:649146/141‑1 (MQ=255)
                                                                                           aaaCACAACAGTTAGCAGGAATGTTGTCATCGTATGTTAAGACTTCTAAAAAGGATGGAATATCTTCTGCAACACAGTCTGAACCGATTCTTTCAATGTATGCTTGCTTTTGAATGTTAATTTCGTCGGCATCATCAAcat                                                   <  1:88398/140‑1 (MQ=255)
                                                                                             acacTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAAcatca                                                 <  1:897586/141‑1 (MQ=255)
                                                                                             acacTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAAcatca                                                 >  2:762839/1‑141 (MQ=255)
                                                                                              cacTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAAcatcat                                                <  4:175304/141‑1 (MQ=255)
                                                                                               acTACCGTTATCAGGAATGTTGTCATCGTATGTTATTAGGGCTAAAATGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAACATCATa                                               >  2:322606/1‑141 (MQ=255)
                                                                                               acTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAACATCATa                                               >  2:33449/1‑141 (MQ=255)
                                                                                               acTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAACATCATa                                               >  1:991244/1‑141 (MQ=255)
                                                                                               acTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAACATCATa                                               <  2:408665/141‑1 (MQ=255)
                                                                                               acTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAACATCATa                                               >  1:514057/1‑141 (MQ=255)
                                                                                               acTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAACATCATa                                               >  2:423287/1‑141 (MQ=255)
                                                                                                cTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAACATCATag                                              <  2:635130/141‑1 (MQ=255)
                                                                                                cTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAACATCATag                                              >  3:226563/1‑141 (MQ=255)
                                                                                                 tACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAACATCATagt                                             <  1:649822/141‑1 (MQ=255)
                                                                                                     gTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAACATCATagtaaag                                         >  1:138769/1‑141 (MQ=255)
                                                                                                      ttATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAACATCATagtaaagt                                        <  4:197974/141‑1 (MQ=255)
                                                                                                      ttATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAACATCATagtaaagt                                        <  2:922291/141‑1 (MQ=255)
                                                                                                       tATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAACATCATagtaaagta                                       <  2:64368/141‑1 (MQ=255)
                                                                                                            ggAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAACATCATAGTAAAGTAAGAAt                                  >  2:569835/1‑141 (MQ=255)
                                                                                                             gAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAACATCATAGTAAAGTAAGAAtt                                 >  2:304462/1‑141 (MQ=255)
                                                                                                                 gttgtCATCGTATGTTAATACTTCTAAGAAGAAAGGAATGTCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAACATCATAGTAAAGTAAGAATTTAAc                             <  2:224478/141‑1 (MQ=255)
                                                                                                                   tgtCATTGTATTTTAATACTTCTAAGAAGATAGGGATGTCTTCTGCAACACATTCTGAACGGATTCTTTCAATGTATGCTTTCTTATGAATGTTAATTTCTTCAGCATCATCAACATCATAGTAATGTAAGTGTTTAAAAg                           <  2:236109/141‑1 (MQ=255)
                                                                                                                    gtCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAACATCATAGTAAAGTAAGAATTTAACAGc                          >  3:318533/1‑141 (MQ=255)
                                                                                                                     tCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAACATCATAGTAAAGTAAGAATTTAACAGCa                         >  3:191717/1‑141 (MQ=255)
                                                                                                                     tCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAACATCATAGTAAAGTAAGAATTTAACAGCa                         >  2:315325/1‑141 (MQ=255)
                                                                                                                      cATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAACATCATAGTAAAGTAAGAATTTAACAGCAt                        >  2:416890/1‑141 (MQ=255)
                                                                                                                      cATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAACATCATAGTAAAGTAAGAATTTAACAGCAt                        >  1:22176/1‑141 (MQ=255)
                                                                                                                      cATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACAGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAACATCATAGTAAAGTAAGAATTTAACAGCAt                        >  2:777772/1‑141 (MQ=255)
                                                                                                                         cGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAACATCATAGTAAAGTAAGAATTTAACAGCATTTg                     >  1:1014686/1‑141 (MQ=255)
                                                                                                                          gTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAACATCATAGTAAAGTAAGAATTTAACAGCATTTgc                    >  2:1075825/1‑141 (MQ=255)
                                                                                                                          gTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAACATCATAGTAAAGTAAGAATTTAACAGCATTTgc                    <  2:607818/141‑1 (MQ=255)
                                                                                                                           tATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAACATCATAGTAAAGTAAGAATTTAACAGCATTTgcg                   <  1:945591/141‑1 (MQ=255)
                                                                                                                              gTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAACATCATAGTAAAGTAAGAATTNAACAGCATTTGCGCCt                <  1:885845/141‑1 (MQ=255)
                                                                                                                                tAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAACATCATAGTAAAGTAAGAATTTAACAGCATTTGCGCCTTg              <  2:824757/141‑1 (MQ=255)
                                                                                                                                 aaTACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAACATCATAGTAAAGTAAGAATTTAACAGCATTTGCGCCTTGt             <  1:316996/141‑1 (MQ=255)
                                                                                                                                    aCTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAACATCATAGTAAAGTAAGAATTTAACAGCATTTGCGCCTTGTTCt          >  1:307001/1‑141 (MQ=255)
                                                                                                                                      ttCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAACATCATAGTAAAGTAAGAATTTAACAGCATTTGCGCCTTGTTCttt        <  4:254017/141‑1 (MQ=255)
                                                                                                                                         tAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAACATCATAGTAAAGTAAGAATTTAACAGCATTTGCGCCTTGTTCTTTTaa     <  1:730906/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                            
ACTTCAACTTTCAATACATCAACGTTAAAGCGTGGTTCTGAGAATAATTTCATTGCTTCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAACATCATAGTAAAGTAAGAATTTAACAGCATTTGCGCCTTGTTCTTTTAAACG  >  USA300TCH1516_ALE/2330360‑2330640

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: