Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 2,360,177 G→T 8.0% intergenic (‑21/+172) infA ← / ← adk Translation initiation factor IF‑1/Adenylate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,360,1770GT 8.0% 384.0 / 4.1 126intergenic (‑21/+172)infA/adkTranslation initiation factor IF‑1/Adenylate kinase
Reads supporting (aligned to +/- strand):  ref base G (53/60);  new base T (11/2);  total (64/62)
Fisher's exact test for biased strand distribution p-value = 1.64e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 131 total aligned reads displayed.

GATTTTACCACTTACGTGAGCTAAAATCTCATGACCATTTTCTAATTCTACTTTAAACATTGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACGGGAATTAATTATCGTTAATTGCCACTCTCATCTATCTAATATGATTAAATCATGCCT  >  USA300TCH1516_ALE/2360037‑2360317
                                                                                                                                            |                                                                                                                                            
gATTTTACCACTTACGTGAGCTAAAATCTCATGACCATTTTCTAATTCTACTTTAAACATTGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTg                                                                                                                                              >  2:891909/1‑141 (MQ=255)
 aTTTTACCACTTACGTGAGCTAAAATCTCATGACCATTTTCTAATTCTACTTTAAACATTGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGc                                                                                                                                             <  1:841646/141‑1 (MQ=255)
 aTTTTACCACTTACGTGAGCTAAAATCTCATGACCATTTTCTAATTCTACTTTAAACATTGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGc                                                                                                                                             <  1:518300/141‑1 (MQ=255)
 aTTTTACCACTTACGTGAGCTAAAATCTCATGACCATTTTCTAATTCTACTTTAAACATTGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGc                                                                                                                                             <  3:194029/141‑1 (MQ=255)
    ttACCACTTACGTGAGCTAAAATCTCATGACCATTTTCTAATTCTACTTTAAACATTGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAAt                                                                                                                                          >  1:996416/1‑141 (MQ=255)
      aCCACTTACGTGAGCTAAAATCTCATGACCATTTTCTAATTCTACTTTAAACATTGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAg                                                                                                                                        >  1:936713/1‑141 (MQ=255)
      aCCACTTACGTGAGCTAAAATCTCATGACCATTTTCTAATTCTACTTTAAACATTGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAg                                                                                                                                        >  3:260157/1‑141 (MQ=255)
       ccACTTACGTGAGCTAAAATCTCATGACCATTTTCTAATTCTACTTTAAACATTGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGt                                                                                                                                       <  1:840592/141‑1 (MQ=255)
          cTTACGTGAGCTAAAATCTCATGACCATTTTCTAATTCTACTTTAAACATTGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAg                                                                                                                                    <  1:1019803/141‑1 (MQ=255)
            tACGTGAGCTAAAATCTCATGACCATTTTCTAATTCTACTTTAAACATTGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGt                                                                                                                                  >  3:21617/1‑141 (MQ=255)
               gTGAGCTAAAATCTCATGACCATTTTCTAATTCTACTTTAAACATTGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAAt                                                                                                                               >  3:202359/1‑141 (MQ=255)
                 gAGCTAAAATCTCATGACCATTTTCTAATTCTACTTTAAACATTGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCg                                                                                                                             >  1:525057/1‑141 (MQ=255)
                      aaaaTCTCATGACCATTTTCTAATTCTACTTTAAACATTGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAAt                                                                                                                        >  3:358767/1‑141 (MQ=255)
                          tctcATGACCATTTTCTAATTCTACTTTAAACATTGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGAc                                                                                                                    <  4:169019/141‑1 (MQ=255)
                          tctcATGACCATTTTCTAATTCTACTTTAAACATTGCGTTCGGTAAAGTATCTAATACAGTAACTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGAc                                                                                                                    >  2:158391/1‑141 (MQ=255)
                           ctcATGACCATTTTCTAATTCTACTTTAAACAATGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAAACa                                                                                                                   >  4:21773/1‑141 (MQ=255)
                             cATGACCATTTTCTAATTCTACTTTAAACATTGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAAc                                                                                                                 >  1:518881/1‑141 (MQ=255)
                              aTGACCATTTTCTAATTCTACTTTAAACATTGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACt                                                                                                                >  1:631883/1‑141 (MQ=255)
                               tGACCATTTTCTAATTCTACTTTAAACATTGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACtt                                                                                                               >  3:53954/1‑141 (MQ=255)
                                 aCCATTTTCTAATTCTACTTTAAACATTGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTa                                                                                                             <  1:423974/141‑1 (MQ=255)
                                 aCCATTTTCTAATTCTACTTTAAACATTGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTa                                                                                                             >  3:168642/1‑141 (MQ=255)
                                   cATTTTCTAATTCTACTTTAAACATTGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTAtt                                                                                                           >  4:243289/1‑141 (MQ=255)
                                      tttCTAATTCTACTTTAAACATTGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGtt                                                                                                        >  2:682149/1‑141 (MQ=255)
                                        tCTAATTCTACTTTAAACATTGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGCTTCACTTCCCTCTTTTTGTAATAGTACGGTAATAGTCAATCGACAACTTTATTGTTAc                                                                                                      >  1:267458/1‑141 (MQ=255)
                                         cTAATTCTACTTTAAACATTGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACg                                                                                                     <  2:726403/141‑1 (MQ=255)
                                         cTAATTCTACTTTAAACATTGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACg                                                                                                     <  4:329357/141‑1 (MQ=255)
                                           aaTTCTACTTTAAACATTTCGTTCGTTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTGGTTTAGCGATTGATTAACTTCCCCCTTTTTGCAATAGTAAGTTAATCGTCGATAGGCTACTTTATTGTTTCGaa                                                                                                   <  2:268276/141‑1 (MQ=255)
                                           aaTTCTACTTTAAACATTGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTTCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGaa                                                                                                   <  2:268271/141‑1 (MQ=255)
                                           aaTTCTACTTTAAACATTGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGaa                                                                                                   <  1:1011954/141‑1 (MQ=255)
                                                 aCTTTAAACATTGCGTTCGGTAAAGTCTCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTTCAATAGTATGGTAATCGTCAATAGACAACTTTATTGTTACGAAtctctc                                                                                             >  2:66299/1‑141 (MQ=255)
                                                 aCTTTAAACATTGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTTTTTTTTTTTTTTAATCGTCAATCGACAACTTTATTGTTACGATTCTATc                                                                                             >  2:457471/1‑141 (MQ=255)
                                                   tttAAACATTGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAGTAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAg                                                                                           <  1:1062563/141‑1 (MQ=255)
                                                   tttAAACATTGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAg                                                                                           >  2:56346/1‑141 (MQ=255)
                                                   tttAAACATTGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAg                                                                                           <  1:1062562/141‑1 (MQ=255)
                                                     tAAACATTGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTg                                                                                         <  4:868/141‑1 (MQ=255)
                                                     tAAACATTGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTg                                                                                         >  1:436654/1‑141 (MQ=255)
                                                       aaCATTGCGTTCGGTAAAGTATCTAATACAGTACCTTATAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGAt                                                                                       >  1:784991/1‑141 (MQ=255)
                                                        aCATTGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGAtt                                                                                      >  1:594320/1‑141 (MQ=255)
                                                         cATTGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTTCTATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTa                                                                                     >  4:112874/1‑141 (MQ=255)
                                                          aTTGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTaa                                                                                    <  4:275062/141‑1 (MQ=255)
                                                          aTTGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTaa                                                                                    <  1:844913/141‑1 (MQ=255)
                                                           ttGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATATTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAAt                                                                                   >  4:185114/1‑141 (MQ=255)
                                                            tGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCATTAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAAtt                                                                                  <  3:418210/141‑1 (MQ=255)
                                                             gCGTTCGNTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAAttt                                                                                 <  2:814960/141‑1 (MQ=255)
                                                             gCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAAttt                                                                                 <  2:900643/141‑1 (MQ=255)
                                                             gCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAAttt                                                                                 <  2:748017/141‑1 (MQ=255)
                                                                  cGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATaa                                                                            >  1:280482/1‑141 (MQ=255)
                                                                  cGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATaa                                                                            <  2:357064/141‑1 (MQ=255)
                                                                  cGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATaa                                                                            <  1:679985/141‑1 (MQ=255)
                                                                   ggTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAg                                                                           >  1:319837/1‑141 (MQ=255)
                                                                     tAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGtt                                                                         <  1:1028593/141‑1 (MQ=255)
                                                                       aaGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTaa                                                                       <  4:260157/141‑1 (MQ=255)
                                                                         gTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAAc                                                                     <  1:111937/141‑1 (MQ=255)
                                                                         gTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAAc                                                                     <  1:298054/141‑1 (MQ=255)
                                                                          tATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACa                                                                    >  2:319566/1‑141 (MQ=255)
                                                                             cTAATACAGTACCTTCTAATTCAATTACATCTTGTGTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTATTACGAATCTATCAGTGATTAATTTTATAAGTAAAACaaaa                                                                 <  4:411983/141‑1 (MQ=255)
                                                                                  aCAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTAc                                                            >  2:674964/1‑141 (MQ=255)
                                                                                   cAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACg                                                           <  1:423451/141‑1 (MQ=255)
                                                                                     gTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACggg                                                         <  2:774563/141‑1 (MQ=255)
                                                                                      tACCTCCTCATTCAAGTCCCTCTTATTCAGCCCTTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACGGGa                                                        <  2:214365/141‑1 (MQ=255)
                                                                                       aCCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACGGGaa                                                       >  3:398121/1‑141 (MQ=255)
                                                                                        ccTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACGGGaat                                                      >  1:194991/1‑141 (MQ=255)
                                                                                           tCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACGGGaattaa                                                   <  2:130670/141‑1 (MQ=255)
                                                                                            cTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACGGGaattaat                                                  <  2:34655/141‑1 (MQ=255)
                                                                                              aaTTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAAAAAAAAAAAAATTACGGGAattaatta                                                <  2:459513/141‑1 (MQ=255)
                                                                                               aTTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTTCATTTGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACGGGAATTAATTAt                                               >  2:92842/1‑141 (MQ=255)
                                                                                               aTTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATATTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACGGGAATTAATTAt                                               >  2:92906/1‑141 (MQ=255)
                                                                                                ttCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTAAAAATAAAAAAAAAATTACGGGAATTAATTATc                                              <  2:517188/141‑1 (MQ=255)
                                                                                                  cAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACGGGAATTAATTATCGt                                            >  1:149338/1‑141 (MQ=255)
                                                                                                  cAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACGGGAATTAATTATCGt                                            >  3:334847/1‑141 (MQ=255)
                                                                                                   aaTTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTTCTTTTTTATGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATACGTTAAACAAAAATTACGGGAATTAATTATCGtt                                           >  4:57454/1‑141 (MQ=255)
                                                                                                    aTTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCATTTTTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACGTGAATTAATTATCGTTa                                          >  4:387444/1‑141 (MQ=255)
                                                                                                      tACATCTTGTTTAGCCATTGATTAACGTCCCCCTTTTTGCATTAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACGGGTATTAATTAGCGTTAAt                                        <  2:389305/141‑1 (MQ=255)
                                                                                                       aCATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACGGGAATTAATTATCGTTAAtt                                       <  2:46148/141‑1 (MQ=255)
                                                                                                        cATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACAGGAATGTTACGAAGCTATCAGTTAGTAATTTTATAAGTTAAACAAAAATTACGGGAATTAATGATCGTTAATTg                                      >  1:102889/1‑141 (MQ=255)
                                                                                                         aTCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACGGGAATTAATTATCGTTAATTGc                                     <  2:1051207/141‑1 (MQ=255)
                                                                                                              ttttAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTATTACGAATCTATCAGTGATTAATTTTAAAAAAAAAAAAAAAATTACGGGAATTAATTATCGTTAATTGCCActc                                <  2:528707/140‑1 (MQ=255)
                                                                                                              gTTTAGCCATTGATTAACTTCCCCCTTTTTTCTTTTTTTTGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACGGGAATTAATTATCGTTAATTGCCActc                                >  2:66360/1‑141 (MQ=255)
                                                                                                              gTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACGGGAATGAATTATCGTTAATTGCCActc                                <  4:329084/141‑1 (MQ=255)
                                                                                                                 tAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACGGGAATTAATTATCGTTAATTGCCACTCTCa                             <  2:215685/141‑1 (MQ=255)
                                                                                                                  aGCCATTGATTAACTTCCCCCTTTTTTCTTTTTTTTGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACGGGAATTAATTATCGTTAATTGCCCCTCTCat                            >  2:687449/1‑141 (MQ=255)
                                                                                                                  aGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACGGGAATTAATTATCGTTAATTGCCACTCTCat                            <  1:375095/141‑1 (MQ=255)
                                                                                                                   gCCATTGATTAACTTCCCCCTTTTTTTTTTTTTTTTTTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACGGGAATTAATTATCTTTAATTGCCCCTCTCatt                           >  4:184884/1‑140 (MQ=255)
                                                                                                                   gCCATTGATTAACTTCCCCCTTTTTGCTATATTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACGGGAATTAATTATCGTTAATTGCCACTCTCatc                           >  2:874325/1‑141 (MQ=255)
                                                                                                                   gCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACGGGAATTAATTATCGTTAATTGCCACTCTCatc                           <  3:295332/141‑1 (MQ=255)
                                                                                                                     cATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACGGGAATTAATTATCGTTAATTGCCACTCTCatcta                         <  2:300905/141‑1 (MQ=255)
                                                                                                                          aTTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACGGGAATTAATTATCGTTAATTGCCACTCTCATCTATCTaa                    <  3:297322/141‑1 (MQ=255)
                                                                                                                          aTTAACTGCCCCCTTTTATCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACAGGAATTAATTATCGTTAATTGCCACTCTCATCTATCTaa                    <  2:914908/141‑1 (MQ=255)
                                                                                                                             aaCTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACGGGAATTAATTATCGTTAATTGCCACTCTCATCTATCTAatat                 >  3:142584/1‑141 (MQ=255)
                                                                                                                             aaCTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACGGGAATTAATTATCGTTAATTGCCACTCTCATCTATCTAatat                 >  1:250717/1‑141 (MQ=255)
                                                                                                                              aCTTCCCCCTCTTTGTAACAGTTGGATAATTGTCAATAGACAAGTTTATAGTTACGAATCTATCAGTAATTAATTTTATAAGTTAAACAAAAATTAAGTCAACTAATTATCGTTAATTGCAATTCTCATCTATCTAataca                >  4:414066/1‑139 (MQ=255)
                                                                                                                               cTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACGGGAATTAATTATCGTTAATTGCCACTCTCATCTATCTAATATGa               <  1:329996/141‑1 (MQ=255)
                                                                                                                                  cccccTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACGGGAATTAATTATCGTTAATTGCCACTCTCATCTATCTAATATGATTa            >  3:437704/1‑141 (MQ=255)
                                                                                                                                    cccTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACGGGAATTAATTATCGTTAATTGCCACTCTCATCTATCTAATATGATTaaa          <  3:440378/141‑1 (MQ=255)
                                                                                                                                      cTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTATTACGAATCTATCAGTGATTAATTTTAAAAAAAAAAAAAAAATTACGGGAATTAATTATCGTTAATTGCCACTCTCATCTATCTAATATGATTAAATc        <  4:56591/141‑1 (MQ=255)
                                                                                                                                       tttttGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACGGGAATTAATTATCGTTAATTGCCACTCTCATCTATCTAATATGATTAAATCa       >  2:121031/1‑141 (MQ=255)
                                                                                                                                         tttGCTTTATTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACGGGAATTAATTATCGTTAATTGCCACTCTCATCTATCTAATATGATTAAATCATg     >  2:818067/1‑141 (MQ=255)
                                                                                                                                           tGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACGGGAATTAATTATCGTTAATTGCCACTCTCATCTATCTAATATGATTAAATCATGcc   <  3:255011/141‑1 (MQ=255)
                                                                                                                                            gCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACGGGAATTAATTATCGTTAATTGCCACTCTCATCTATCTAATATGATTAAATCATGCCt  <  1:863938/141‑1 (MQ=255)
                                                                                                                                            gCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTAATTAATTTTATAAGTTAAACAAAAATTACGGGAATTAATTATCGTTAATTGCCACTCTCATCTATCTAATATGATTAAATCATGCCt  <  1:212116/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                            
GATTTTACCACTTACGTGAGCTAAAATCTCATGACCATTTTCTAATTCTACTTTAAACATTGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACGGGAATTAATTATCGTTAATTGCCACTCTCATCTATCTAATATGATTAAATCATGCCT  >  USA300TCH1516_ALE/2360037‑2360317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: