Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 2,360,180 A→T 8.1% intergenic (‑24/+169) infA ← / ← adk Translation initiation factor IF‑1/Adenylate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,360,1800AT 8.1% 362.1 / 3.5 130intergenic (‑24/+169)infA/adkTranslation initiation factor IF‑1/Adenylate kinase
Reads supporting (aligned to +/- strand):  ref base A (57/58);  new base T (11/2);  total (68/62)
Fisher's exact test for biased strand distribution p-value = 1.92e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 133 total aligned reads displayed.

TTACCACTTACGTGAGCTAAAATCTCATGACCATTTTCTAATTCTACTTTAAACATTGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACGGGAATTAATTATCGTTAATTGCCACTCTCATCTATCTAATATGATTAAATCATGCCTCAC  >  USA300TCH1516_ALE/2360041‑2360320
                                                                                                                                           |                                                                                                                                            
ttACCACTTACGTGAGCTAAAATCTCATGACCATTTTCTAATTCTACTTTAAACATTGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAAt                                                                                                                                             >  1:996416/1‑141 (MQ=255)
  aCCACTTACGTGAGCTAAAATCTCATGACCATTTTCTAATTCTACTTTAAACATTGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAg                                                                                                                                           >  1:936713/1‑141 (MQ=255)
  aCCACTTACGTGAGCTAAAATCTCATGACCATTTTCTAATTCTACTTTAAACATTGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAg                                                                                                                                           >  3:260157/1‑141 (MQ=255)
   ccACTTACGTGAGCTAAAATCTCATGACCATTTTCTAATTCTACTTTAAACATTGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGt                                                                                                                                          >  3:109638/1‑141 (MQ=255)
      cTTACGTGAGCTAAAATCTCATGACCATTTTCTAATTCTACTTTAAACATTGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAg                                                                                                                                       <  1:1019803/141‑1 (MQ=255)
        tACGTGAGCTAAAATCTCATGACCATTTTCTAATTCTACTTTAAACATTGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGt                                                                                                                                     >  3:21617/1‑141 (MQ=255)
           gTGAGCTAAAATCTCATGACCATTTTCTAATTCTACTTTAAACATTGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAAt                                                                                                                                  >  3:202359/1‑141 (MQ=255)
             gAGCTAAAATCTCATGACCATTTTCTAATTCTACTTTAAACATTGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCg                                                                                                                                >  1:525057/1‑141 (MQ=255)
                  aaaaTCTCATGACCATTTTCTAATTCTACTTTAAACATTGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAAt                                                                                                                           >  3:358767/1‑141 (MQ=255)
                    aaTCTCATGACCATTTTCTAATTCTACTTTAAACATTGCGTTCGGTAAAGTAGCTAATACAGTACCTTCTAGTTCAGTTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAg                                                                                                                         >  2:643454/1‑141 (MQ=255)
                     aTCTCATGACCATTTTCTAATTCTACTTTAAACATTGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGa                                                                                                                        >  1:291720/1‑141 (MQ=255)
                     aTCTCATGACCATTTTCTAATTCTACTTTAAACATTGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGa                                                                                                                        >  1:470449/1‑141 (MQ=255)
                      tctcATGACCATTTTCTAATTCTACTTTAAACATTGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGAc                                                                                                                       <  4:169019/141‑1 (MQ=255)
                      tctcATGACCATTTTCTAATTCTACTTTAAACATTGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGAc                                                                                                                       <  2:908130/141‑1 (MQ=255)
                      tctcATGACCATTTTCTAATTCTACTTTAAACATTGCGTTCGGTAAAGTATCTAATACAGTAACTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGAc                                                                                                                       >  2:158391/1‑141 (MQ=255)
                       ctcATGACCATTTTCTAATTCTACTTTAAACAATGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAAACa                                                                                                                      >  4:21773/1‑141 (MQ=255)
                         cATGACCATTTTCTAATTCTACTTTAAACATTGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAAc                                                                                                                    >  1:518881/1‑141 (MQ=255)
                           tGACCATTTTCTAATTCTACTTTAAACATTGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACtt                                                                                                                  >  3:53954/1‑141 (MQ=255)
                              ccATTTTCTAATTCTACTTTAAACATTGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTAt                                                                                                               >  2:394879/1‑141 (MQ=255)
                               cATTTTCTAATTCTACTTTAAACATTGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTAtt                                                                                                              >  4:243289/1‑141 (MQ=255)
                                  tttCTAATTCTACTTTAAACATTGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGtt                                                                                                           >  2:682149/1‑141 (MQ=255)
                                    tCTAATTCTACTTTAAACATTGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGCTTCACTTCCCTCTTTTTGTAATAGTACGGTAATAGTCAATCGACAACTTTATTGTTAc                                                                                                         >  1:267458/1‑141 (MQ=255)
                                     cTAATTCTACTTTAAACATTGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTTCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACg                                                                                                        <  3:349647/141‑1 (MQ=255)
                                     cTAATTCTACTTTAAACATTGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACg                                                                                                        <  4:329357/141‑1 (MQ=255)
                                       aaTTCTACTTTAAACATTTCGTTCGTTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTGGTTTAGCGATTGATTAACTTCCCCCTTTTTGCAATAGTAAGTTAATCGTCGATAGGCTACTTTATTGTTTCGaa                                                                                                      <  2:268276/141‑1 (MQ=255)
                                       aaTTCTACTTTAAACATTGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTTCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGaa                                                                                                      <  2:268271/141‑1 (MQ=255)
                                       aaTTCTACTTTAAACATTGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGaa                                                                                                      <  1:1011954/141‑1 (MQ=255)
                                        aTTCTACTTTAAACATTGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAAt                                                                                                     >  3:82783/1‑141 (MQ=255)
                                             aCTTTAAACATTGCGTTCGGTAAAGTCTCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTTCAATAGTATGGTAATCGTCAATAGACAACTTTATTGTTACGAAtctctc                                                                                                >  2:66299/1‑141 (MQ=255)
                                             aCTTTAAACATTGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTTTTTTTTTTTTTTAATCGTCAATCGACAACTTTATTGTTACGATTCTATc                                                                                                >  2:457471/1‑141 (MQ=255)
                                               tttAAACATTGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAGTAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAg                                                                                              <  1:1062563/141‑1 (MQ=255)
                                               tttAAACATTGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAg                                                                                              <  1:1062562/141‑1 (MQ=255)
                                                 tAAACATTGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTg                                                                                            <  4:868/141‑1 (MQ=255)
                                                 tAAACATTGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTg                                                                                            >  1:436654/1‑141 (MQ=255)
                                                   aaCATTGCGTTCGGTAAAGTATCTAATACAGTACCTTATAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGAt                                                                                          >  1:784991/1‑141 (MQ=255)
                                                    aCATTGCGTTCGGTAAAGTATCTAATCCAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGAtt                                                                                         <  2:859194/141‑1 (MQ=255)
                                                    aCATTGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGAtt                                                                                         >  1:594320/1‑141 (MQ=255)
                                                      aTTGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTaa                                                                                       <  4:275062/141‑1 (MQ=255)
                                                      aTTGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTaa                                                                                       <  1:844913/141‑1 (MQ=255)
                                                      aTTGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTaa                                                                                       <  3:197689/141‑1 (MQ=255)
                                                       ttGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATATTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAAt                                                                                      >  4:185114/1‑141 (MQ=255)
                                                        tGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCATTAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAAtt                                                                                     <  3:418210/141‑1 (MQ=255)
                                                        tGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAAtt                                                                                     >  1:537841/1‑141 (MQ=255)
                                                         gCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAAttt                                                                                    <  2:748017/141‑1 (MQ=255)
                                                             tCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTtata                                                                                >  1:734732/1‑141 (MQ=255)
                                                              cGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATaa                                                                               <  1:679985/141‑1 (MQ=255)
                                                               ggTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAg                                                                              >  1:319837/1‑141 (MQ=255)
                                                                 tAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGtt                                                                            <  1:1028593/141‑1 (MQ=255)
                                                                   aaGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTaa                                                                          <  4:260157/141‑1 (MQ=255)
                                                                     gTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAAc                                                                        <  1:298054/141‑1 (MQ=255)
                                                                     gTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAAc                                                                        <  1:111937/141‑1 (MQ=255)
                                                                      tATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACa                                                                       >  2:319566/1‑141 (MQ=255)
                                                                         cTAATACAGTACCTTCTAATTCAATTACATCTTGTGTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTATTACGAATCTATCAGTGATTAATTTTATAAGTAAAACaaaa                                                                    <  4:411983/141‑1 (MQ=255)
                                                                              aCAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTTTTTTTTTTTGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTAc                                                               >  2:675015/1‑141 (MQ=255)
                                                                               cAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACg                                                              <  1:423451/141‑1 (MQ=255)
                                                                                 gTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACggg                                                            <  2:774563/141‑1 (MQ=255)
                                                                                  tACCTCCTCATTCAAGTCCCTCTTATTCAGCCCTTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACGGGa                                                           <  2:214365/141‑1 (MQ=255)
                                                                                    ccTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACGGGaat                                                         >  1:194991/1‑141 (MQ=255)
                                                                                       tCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACGGGaattaa                                                      <  2:130670/141‑1 (MQ=255)
                                                                                        cTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACGGGaattaat                                                     <  2:34655/141‑1 (MQ=255)
                                                                                           aTTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTTCATTTGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACGGGAATTAATTAt                                                  >  2:92842/1‑141 (MQ=255)
                                                                                            ttCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTAAAAATAAAAAAAAAATTACGGGAATTAATTATc                                                 <  2:517188/141‑1 (MQ=255)
                                                                                              cAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACGGGAATTAATTATCGt                                               >  1:149338/1‑141 (MQ=255)
                                                                                              cAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACGGGAATTAATTATCGt                                               >  3:334847/1‑141 (MQ=255)
                                                                                               aaTTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTTCTTTTTTATGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATACGTTAAACAAAAATTACGGGAATTAATTATCGtt                                              >  4:57454/1‑141 (MQ=255)
                                                                                                aTTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCATTTTTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACGTGAATTAATTATCGTTa                                             >  4:387444/1‑141 (MQ=255)
                                                                                                  tACATCTTGTTTAGCCATTGATTAACGTCCCCCTTTTTGCATTAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACGGGTATTAATTAGCGTTAAt                                           <  2:389305/141‑1 (MQ=255)
                                                                                                   aCATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACGGGAATTAATTATCGTTAAtt                                          <  2:46148/141‑1 (MQ=255)
                                                                                                    cATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACAGGAATGTTACGAAGCTATCAGTTAGTAATTTTATAAGTTAAACAAAAATTACGGGAATTAATGATCGTTAATTg                                         >  1:102889/1‑141 (MQ=255)
                                                                                                     aTCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACGGGAATTAATTATCGTTAATTGc                                        <  2:1051207/141‑1 (MQ=255)
                                                                                                          ttttAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTATTACGAATCTATCAGTGATTAATTTTAAAAAAAAAAAAAAAATTACGGGAATTAATTATCGTTAATTGCCActc                                   <  2:528707/140‑1 (MQ=255)
                                                                                                          gTTTAGCCATTGATTAACTTCCCCCTTTTTTCTTTTTTTTGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACGGGAATTAATTATCGTTAATTGCCActc                                   >  2:66360/1‑141 (MQ=255)
                                                                                                          gTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACGGGAATGAATTATCGTTAATTGCCActc                                   <  4:329084/141‑1 (MQ=255)
                                                                                                             tAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACGGGAATTAATTATCGTTAATTGCCACTCTCa                                <  2:215685/141‑1 (MQ=255)
                                                                                                              aGCCATTGATTAACTTCCCCCTTTTTTCTTTTTTTTGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACGGGAATTAATTATCGTTAATTGCCCCTCTCat                               >  2:687449/1‑141 (MQ=255)
                                                                                                              aGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACGGGAATTAATTATCGTTAATTGCCACTCTCat                               <  1:375197/141‑1 (MQ=255)
                                                                                                              aGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACGGGAATTAATTATCGTTAATTGCCACTCTCat                               <  1:375095/141‑1 (MQ=255)
                                                                                                               gCCATTGATTAACTTCCCCCTTTTTTTTTTTTTTTTTTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACGGGAATTAATTATCTTTAATTGCCCCTCTCatt                              >  4:184884/1‑140 (MQ=255)
                                                                                                               gCCATTGATTAACTTCCCCCTTTTTGCTATATTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACGGGAATTAATTATCGTTAATTGCCACTCTCatc                              >  2:874325/1‑141 (MQ=255)
                                                                                                               gCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACGGGAATTAATTATCGTTAATTGCCACTCTCatc                              <  3:295332/141‑1 (MQ=255)
                                                                                                                ccATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGCTTCATTTTATAAGCTACACAAAAATTCCGGAACTTCCTTATCGTCAATTGCCACTCTCatct                             >  3:434885/1‑141 (MQ=255)
                                                                                                                      aTTAACTGCCCCCTTTTATCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACAGGAATTAATTATCGTTAATTGCCACTCTCATCTATCTaa                       <  2:914908/141‑1 (MQ=255)
                                                                                                                       ttAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACGGGAATTAATTATCGTTAATTGCCACTCTCATCTATCTAat                      <  2:760937/141‑1 (MQ=255)
                                                                                                                         aaCTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACGGGAATTAATTATCGTTAATTGCCACTCTCATCTATCTAatat                    >  3:266088/1‑141 (MQ=255)
                                                                                                                         aaCTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACGGGAATTAATTATCGTTAATTGCCACTCTCATCTATCTAatat                    >  3:142584/1‑141 (MQ=255)
                                                                                                                         aaCTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACGGGAATTAATTATCGTTAATTGCCACTCTCATCTATCTAatat                    >  1:250717/1‑141 (MQ=255)
                                                                                                                          aCTTCCCCCTCTTTGTAACAGTTGGATAATTGTCAATAGACAAGTTTATAGTTACGAATCTATCAGTAATTAATTTTATAAGTTAAACAAAAATTAAGTCAACTAATTATCGTTAATTGCAATTCTCATCTATCTAataca                   >  4:414066/1‑139 (MQ=255)
                                                                                                                              cccccTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACGGGAATTAATTATCGTTAATTGCCACTCTCATCTATCTAATATGATTa               >  3:437704/1‑141 (MQ=255)
                                                                                                                                cccTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACGGGAATTAATTATCGTTAATTGCCACTCTCATCTATCTAATATGATTaaa             <  1:863838/141‑1 (MQ=255)
                                                                                                                                 ccTTTTTGCAGTAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCATTGATTAATTTTATAAGTTAAACAAAAATTACGGGAATTAATTATCGTTAATTGCCACTCTCATCTATCTAATATGATTAAAt            <  2:302833/141‑1 (MQ=255)
                                                                                                                                  cTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTATTACGAATCTATCAGTGATTAATTTTAAAAAAAAAAAAAAAATTACGGGAATTAATTATCGTTAATTGCCACTCTCATCTATCTAATATGATTAAATc           <  4:56591/141‑1 (MQ=255)
                                                                                                                                   tttttGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACGGGAATTAATTATCGTTAATTGCCACTCTCATCTATCTAATATGATTAAATCa          >  2:121031/1‑141 (MQ=255)
                                                                                                                                     tttGCTTTATTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACGGGAATTAATTATCGTTAATTGCCACTCTCATCTATCTAATATGATTAAATCATg        >  2:818067/1‑141 (MQ=255)
                                                                                                                                        gCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACGGGAATTAATTATCGTTAATTGCCACTCTCATCTATCTAATATGATTAAATCATGCCt     <  1:863938/141‑1 (MQ=255)
                                                                                                                                        gCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTAATTAATTTTATAAGTTAAACAAAAATTACGGGAATTAATTATCGTTAATTGCCACTCTCATCTATCTAATATGATTAAATCATGCCt     <  1:212116/141‑1 (MQ=255)
                                                                                                                                         cAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACGGGAATTAATTATCGTTAATTGCCACTCTCATCTATCTAATATGATTAAATCATGCCTc    >  1:963905/1‑141 (MQ=255)
                                                                                                                                         cAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACGGGAATTAATTATCGTTAATTGCCACTCTCATCTATCTAATATGATTAAATCATGCCTc    >  3:59533/1‑141 (MQ=255)
                                                                                                                                         cAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACGGGAATTAATTATCGTTAATTGCCACTCTCATCTATCGAATATGATTAAATCATGCCTc    >  1:275828/1‑141 (MQ=255)
                                                                                                                                          aaTAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACGGGAATTAATTATCGTTAATTGCCACTCTCATCTATCTAATATGATTAAATCATGCCTCa   <  3:127712/141‑1 (MQ=255)
                                                                                                                                           aTAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACGGGAATTAATTATCGTTAATTGCCACTCTCATCTATCTAATATGATTAAATCATGCCTCAc  <  2:252129/141‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                            
TTACCACTTACGTGAGCTAAAATCTCATGACCATTTTCTAATTCTACTTTAAACATTGCGTTCGGTAAAGTATCTAATACAGTACCTTCTAATTCAATTACATCTTGTTTAGCCATTGATTAACTTCCCCCTTTTTGCAATAGTAAGGTAATCGTCAATAGACAACTTTATTGTTACGAATCTATCAGTGATTAATTTTATAAGTTAAACAAAAATTACGGGAATTAATTATCGTTAATTGCCACTCTCATCTATCTAATATGATTAAATCATGCCTCAC  >  USA300TCH1516_ALE/2360041‑2360320

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: