Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 2,372,413 C→G 5.8% intergenic (+164/+28) USA300TCH1516_02262 → / ← pbuG hypothetical protein/Guanine/hypoxanthine permease PbuG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,372,4130CG 5.8% 508.2 / 17.5 152intergenic (+164/+28)USA300TCH1516_02262/pbuGhypothetical protein/Guanine/hypoxanthine permease PbuG
Reads supporting (aligned to +/- strand):  ref base C (71/72);  new base G (6/3);  total (77/75)
Fisher's exact test for biased strand distribution p-value = 4.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 153 total aligned reads displayed.

TATAATATAGAAGAAACACGTCCAACAATTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAAAATAA  >  USA300TCH1516_ALE/2372275‑2372553
                                                                                                                                          |                                                                                                                                            
tataatataGAAGAAACACGTCCAACAATTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATTGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAACGACTACCAATCAtt                                                                                                                                           <  1:977892/141‑1 (MQ=255)
 ataatataGAAGAAACACGTCCAACAATTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCAtt                                                                                                                                           <  1:890099/141‑1 (MQ=255)
  taatataGAAGAAACACGTCCAACAATTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTg                                                                                                                                          >  4:325817/1‑141 (MQ=255)
  taatataGAAGAAACACGTCCAACAATTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTg                                                                                                                                          >  2:631782/1‑141 (MQ=255)
    atataGAAGAAACACGTCCAACAATTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGc                                                                                                                                        <  1:248716/141‑1 (MQ=255)
    atataGAAGAAACACGTCCAACAATTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCCCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGc                                                                                                                                        <  3:349753/141‑1 (MQ=255)
      ataGAAGAAACACGTCCAACAATTTTAAAAGAAGTATATTTATATTGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAg                                                                                                                                      >  1:615833/1‑141 (MQ=255)
      ataGAAGAAACACGTCCAACAATTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATTGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAACGACTGCCAATGATTGGTAGt                                                                                                                                     <  1:390554/141‑1 (MQ=255)
      ataGAAGAAACACGTCCAACAATTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAg                                                                                                                                      >  3:13670/1‑141 (MQ=255)
      ataGAAGAAACACCACCAACAAATTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTGTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAg                                                                                                                                      <  2:1032706/141‑1 (MQ=255)
        agaagaAACACGTCCAACAATTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTGCCAATGATTGGTAGTc                                                                                                                                    <  1:544803/141‑1 (MQ=255)
        agaagaAACACGTCCAACAATTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTc                                                                                                                                    <  3:23480/141‑1 (MQ=255)
        agaagaAACACGTCCAACAATTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTc                                                                                                                                    <  3:222193/141‑1 (MQ=255)
        agaagaAACACGTCCAACAATTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTc                                                                                                                                    <  3:364676/141‑1 (MQ=255)
        agaagaAACACGTCCAACAATTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTc                                                                                                                                    >  2:385555/1‑141 (MQ=255)
        agaagaAACACGTCCAACAATTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTc                                                                                                                                    <  1:419276/141‑1 (MQ=255)
            gaaACACGTCCAACAATTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGttt                                                                                                                                <  3:457567/141‑1 (MQ=255)
             aaaCACGTCCAACAATTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGtttt                                                                                                                               <  2:368400/141‑1 (MQ=255)
             aaaCACGTCCAACAATTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGtttt                                                                                                                               <  1:912023/141‑1 (MQ=255)
               acacGTCCAACAATTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATTGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAACGACTACCAATCATTGGCAGTCGTTTTTtg                                                                                                                            <  3:296177/141‑1 (MQ=255)
                    tCCAACAATTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTc                                                                                                                        <  3:140215/141‑1 (MQ=255)
                    tCCAACAATGTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTc                                                                                                                        <  2:511111/141‑1 (MQ=255)
                      caacaaTTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTg                                                                                                                      >  4:442627/1‑141 (MQ=255)
                        acaaTTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGa                                                                                                                    <  2:529466/141‑1 (MQ=255)
                        acaaTTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGa                                                                                                                    <  1:608287/141‑1 (MQ=255)
                        acaaTTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGa                                                                                                                    >  2:1002308/1‑141 (MQ=255)
                          aaTTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGAtt                                                                                                                  >  2:485978/1‑141 (MQ=255)
                           aTTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGAttt                                                                                                                 <  3:355513/141‑1 (MQ=255)
                           aTTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGAttt                                                                                                                 <  1:449743/141‑1 (MQ=255)
                                aaaaGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCa                                                                                                            >  1:451975/1‑141 (MQ=255)
                                 aaaGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCAt                                                                                                           >  4:389167/1‑141 (MQ=255)
                                   agaagTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGa                                                                                                         >  1:407392/1‑141 (MQ=255)
                                   agaacTAAATTTATATCAGATATTTAAAGACATTCGTAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACAACAAAAAAAAAAACGACTACCAATCATTGGTAGTCGTTTTTTGTGTCTGGATTTAACCATGa                                                                                                         <  2:949168/141‑1 (MQ=255)
                                     aagTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAAc                                                                                                       <  4:206722/141‑1 (MQ=255)
                                          tatTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCACTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAata                                                                                                  <  2:53050/141‑1 (MQ=255)
                                           atTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAatat                                                                                                 >  1:82913/1‑141 (MQ=255)
                                                tatCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGt                                                                                            >  1:437556/1‑141 (MQ=255)
                                                 atCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTa                                                                                           >  2:556446/1‑141 (MQ=255)
                                                  tCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTAc                                                                                          >  1:552527/1‑141 (MQ=255)
                                                   cGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACa                                                                                         >  2:605394/1‑141 (MQ=255)
                                                   cGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACa                                                                                         <  2:693046/141‑1 (MQ=255)
                                                     gATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAga                                                                                       <  1:1034633/141‑1 (MQ=255)
                                                        atTTAAAAACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTTGATTTAACCATGAACAAATATGAAGTACAgaatg                                                                                    <  3:38778/141‑1 (MQ=255)
                                                           tAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAgaatgaat                                                                                 >  1:704675/1‑141 (MQ=255)
                                                             aaGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAg                                                                               >  1:675255/1‑141 (MQ=255)
                                                              aGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGt                                                                              >  2:716727/1‑141 (MQ=255)
                                                              aGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGt                                                                              >  3:394392/1‑141 (MQ=255)
                                                              aGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGt                                                                              >  2:974573/1‑141 (MQ=255)
                                                               gACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTa                                                                             <  2:407392/141‑1 (MQ=255)
                                                                 cATTCATAATATCGCTTTATGTTACTCCGCTTCTCATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATCCAAATATACGAAGGATAGATTGAATAGTAcc                                                                           <  2:273899/141‑1 (MQ=255)
                                                                  aTTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCa                                                                          <  2:787770/141‑1 (MQ=255)
                                                                  aTTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCa                                                                          >  1:795616/1‑141 (MQ=255)
                                                                   ttCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCAt                                                                         >  2:5693/1‑141 (MQ=255)
                                                                   ttCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCAt                                                                         >  4:284212/1‑141 (MQ=255)
                                                                      ataataTCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTaa                                                                      >  2:757580/1‑141 (MQ=255)
                                                                      ataataTCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTaa                                                                      >  3:9481/1‑141 (MQ=255)
                                                                           atCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAatacat                                                                 >  2:232284/1‑141 (MQ=255)
                                                                             cGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAatacatac                                                               >  2:179764/1‑141 (MQ=255)
                                                                               cTTTATGTTATTTCGCTTCATATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATacatacat                                                             >  2:600321/1‑141 (MQ=255)
                                                                                tttATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACAtt                                                            <  1:32818/141‑1 (MQ=255)
                                                                                tttATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACAtt                                                            >  1:359594/1‑141 (MQ=255)
                                                                                tttATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACAtt                                                            <  2:800451/141‑1 (MQ=255)
                                                                                  tATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAatacatccgataa                                                          <  3:23847/141‑8 (MQ=255)
                                                                                  tATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTAt                                                          <  1:480354/141‑1 (MQ=255)
                                                                                         tttCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGt                                                   <  1:1002308/141‑1 (MQ=255)
                                                                                         tttCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGt                                                   <  1:372398/141‑1 (MQ=255)
                                                                                         tttCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGt                                                   <  2:342022/141‑1 (MQ=255)
                                                                                                tcttATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTActtctt                                            <  3:407604/141‑1 (MQ=255)
                                                                                                  ttATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTa                                          <  2:442963/141‑1 (MQ=255)
                                                                                                  ttATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTAGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAAAATGCCTAGTACCATTAATACATACATTATCGGATGTACTTCTTTa                                          <  2:227168/141‑1 (MQ=255)
                                                                                                    aTTTTATCCTTACTTCAAAGAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGt                                       <  3:270325/141‑1 (MQ=255)
                                                                                                     ttttATCCTTACTACAAAGAAAAAAGCGACTACCAATGATTGGTAGTCGTTTTTTGTGTCTGGCTTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGt                                       >  2:922532/1‑141 (MQ=255)
                                                                                                      tttATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAACGAATAGTACCATTACTACATACATTATCGGACGTACTTATTTATGtt                                      >  2:1036659/1‑141 (MQ=255)
                                                                                                       ttATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGttt                                     <  2:179059/141‑1 (MQ=255)
                                                                                                        tATCCTCACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCAGATCATCTTCTTTATGtttt                                    >  1:332609/1‑141 (MQ=255)
                                                                                                         aTCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGttttt                                   <  1:211058/141‑1 (MQ=255)
                                                                                                            cTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTag                                >  1:999136/1‑141 (MQ=255)
                                                                                                             ttACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTaga                               >  2:284068/1‑141 (MQ=255)
                                                                                                             ttACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTaga                               >  1:660536/1‑141 (MQ=255)
                                                                                                              tACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTagag                              >  1:271770/1‑141 (MQ=255)
                                                                                                               aCTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAgaga                             >  2:1062404/1‑141 (MQ=255)
                                                                                                               aCTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAgaga                             >  2:326698/1‑141 (MQ=255)
                                                                                                                    aaaGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGc                        <  1:410136/141‑1 (MQ=255)
                                                                                                                    aaaGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGc                        >  1:1077095/1‑141 (MQ=255)
                                                                                                                      aGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCAt                      >  1:703687/1‑141 (MQ=255)
                                                                                                                      aGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCAt                      <  4:280748/141‑1 (MQ=255)
                                                                                                                       gAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCAtt                     <  4:385297/141‑1 (MQ=255)
                                                                                                                         aaaaaaCGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGt                   <  2:445094/141‑1 (MQ=255)
                                                                                                                          aaaaaCGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTa                  >  3:368362/1‑141 (MQ=255)
                                                                                                                          aaaaaCGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTa                  >  3:17465/1‑141 (MQ=255)
                                                                                                                           aaaaCGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTaa                 >  2:993986/1‑141 (MQ=255)
                                                                                                                            aaaCGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAAt                <  2:726372/141‑1 (MQ=255)
                                                                                                                             aaCGACTGCCAATGATTGGTAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAAtt               >  1:48361/1‑141 (MQ=255)
                                                                                                                              aCGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGCTAAGCATTGTAATTg              >  4:339303/1‑141 (MQ=255)
                                                                                                                              aCGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTg              >  1:1080401/1‑141 (MQ=255)
                                                                                                                              aCGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTg              <  4:173222/141‑1 (MQ=255)
                                                                                                                                   tACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAg         >  1:905071/1‑141 (MQ=255)
                                                                                                                                     ccAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTATCCAAAAACAAATATTAATTACATAATTAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGaa       <  1:99231/141‑1 (MQ=255)
                                                                                                                                      cAATGATTGGTAGTCGTTTTTTTTGTCTGGTTTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGaaa      >  2:136547/1‑141 (MQ=255)
                                                                                                                                          cATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATGAATACATATATGATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAaaataa  >  1:214198/1‑141 (MQ=255)
                                                                                                                                          |                                                                                                                                            
TATAATATAGAAGAAACACGTCCAACAATTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAAAATAA  >  USA300TCH1516_ALE/2372275‑2372553

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: