Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | USA300TCH1516_ALE | 2,372,413 | C→G | 5.8% | intergenic (+164/+28) | USA300TCH1516_02262 → / ← pbuG | hypothetical protein/Guanine/hypoxanthine permease PbuG |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | USA300TCH1516_ALE | 2,372,413 | 0 | C | G | 5.8% | 508.2 / 17.5 | 152 | intergenic (+164/+28) | USA300TCH1516_02262/pbuG | hypothetical protein/Guanine/hypoxanthine permease PbuG |
Reads supporting (aligned to +/- strand): ref base C (71/72); new base G (6/3); total (77/75) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 4.95e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 153 total aligned reads displayed.
TATAATATAGAAGAAACACGTCCAACAATTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAAAATAA > USA300TCH1516_ALE/2372275‑2372553 | tataatataGAAGAAACACGTCCAACAATTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATTGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAG‑AAAAAACGACTACCAATCAtt < 1:977892/141‑1 (MQ=255) ataatataGAAGAAACACGTCCAACAATTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCAtt < 1:890099/141‑1 (MQ=255) taatataGAAGAAACACGTCCAACAATTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTg > 4:325817/1‑141 (MQ=255) taatataGAAGAAACACGTCCAACAATTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTg > 2:631782/1‑141 (MQ=255) atataGAAGAAACACGTCCAACAATTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGc < 1:248716/141‑1 (MQ=255) atataGAAGAAACACGTCCAACAATTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCCCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGc < 3:349753/141‑1 (MQ=255) ataGAAGAAACACGTCCAACAATTTTAAAAGAAGTATATTTATATTGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAg > 1:615833/1‑141 (MQ=255) ataGAAGAAACACGTCCAACAATTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATTGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAG‑AAAAAACGACTGCCAATGATTGGTAGt < 1:390554/141‑1 (MQ=255) ataGAAGAAACACGTCCAACAATTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAg > 3:13670/1‑141 (MQ=255) ataGAAGAAACACCACCAACAAATTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTGTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAg < 2:1032706/141‑1 (MQ=255) agaagaAACACGTCCAACAATTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTGCCAATGATTGGTAGTc < 1:544803/141‑1 (MQ=255) agaagaAACACGTCCAACAATTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTc < 3:23480/141‑1 (MQ=255) agaagaAACACGTCCAACAATTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTc < 3:222193/141‑1 (MQ=255) agaagaAACACGTCCAACAATTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTc < 3:364676/141‑1 (MQ=255) agaagaAACACGTCCAACAATTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTc > 2:385555/1‑141 (MQ=255) agaagaAACACGTCCAACAATTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTc < 1:419276/141‑1 (MQ=255) gaaACACGTCCAACAATTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGttt < 3:457567/141‑1 (MQ=255) aaaCACGTCCAACAATTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGtttt < 2:368400/141‑1 (MQ=255) aaaCACGTCCAACAATTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGtttt < 1:912023/141‑1 (MQ=255) acacGTCCAACAATTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATTGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAG‑AAAAAACGACTACCAATCATTGGCAGTCGTTTTTtg < 3:296177/141‑1 (MQ=255) tCCAACAATTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTc < 3:140215/141‑1 (MQ=255) tCCAACAATGTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTc < 2:511111/141‑1 (MQ=255) caacaaTTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTg > 4:442627/1‑141 (MQ=255) acaaTTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGa < 2:529466/141‑1 (MQ=255) acaaTTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGa < 1:608287/141‑1 (MQ=255) acaaTTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGa > 2:1002308/1‑141 (MQ=255) aaTTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGAtt > 2:485978/1‑141 (MQ=255) aTTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGAttt < 3:355513/141‑1 (MQ=255) aTTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGAttt < 1:449743/141‑1 (MQ=255) aaaaGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCa > 1:451975/1‑141 (MQ=255) aaaGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCAt > 4:389167/1‑141 (MQ=255) agaagTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGa > 1:407392/1‑141 (MQ=255) agaacTAAATTTATATCAGATATTTAAAGACATTCGTAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACAACAAAAAAAAAAACGACTACCAATCATTGGTAGTCGTTTTTTGTGTCTGGATTTAACCATGa < 2:949168/141‑1 (MQ=255) aagTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAAc < 4:206722/141‑1 (MQ=255) tatTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCACTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAata < 2:53050/141‑1 (MQ=255) atTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAatat > 1:82913/1‑141 (MQ=255) tatCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGt > 1:437556/1‑141 (MQ=255) atCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTa > 2:556446/1‑141 (MQ=255) tCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTAc > 1:552527/1‑141 (MQ=255) cGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACa > 2:605394/1‑141 (MQ=255) cGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACa < 2:693046/141‑1 (MQ=255) gATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAga < 1:1034633/141‑1 (MQ=255) atTTAAAAACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTTGATTTAACCATGAACAAATATGAAGTACAgaatg < 3:38778/141‑1 (MQ=255) tAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAgaatgaat > 1:704675/1‑141 (MQ=255) aaGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAg > 1:675255/1‑141 (MQ=255) aGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGt > 2:716727/1‑141 (MQ=255) aGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGt > 3:394392/1‑141 (MQ=255) aGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGt > 2:974573/1‑141 (MQ=255) gACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTa < 2:407392/141‑1 (MQ=255) cATTCATAATATCGCTTTATGTTACTCCGCTTCTCATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATCCAAATATACGAAGGATAGATTGAATAGTAcc < 2:273899/141‑1 (MQ=255) aTTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCa < 2:787770/141‑1 (MQ=255) aTTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCa > 1:795616/1‑141 (MQ=255) ttCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCAt > 2:5693/1‑141 (MQ=255) ttCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCAt > 4:284212/1‑141 (MQ=255) ataataTCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTaa > 2:757580/1‑141 (MQ=255) ataataTCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTaa > 3:9481/1‑141 (MQ=255) atCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAatacat > 2:232284/1‑141 (MQ=255) cGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAatacatac > 2:179764/1‑141 (MQ=255) cTTTATGTTATTTCGCTTCATATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATacatacat > 2:600321/1‑141 (MQ=255) tttATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACAtt < 1:32818/141‑1 (MQ=255) tttATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACAtt > 1:359594/1‑141 (MQ=255) tttATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACAtt < 2:800451/141‑1 (MQ=255) tATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAatacatccgataa < 3:23847/141‑8 (MQ=255) tATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTAt < 1:480354/141‑1 (MQ=255) tttCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGt < 1:1002308/141‑1 (MQ=255) tttCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGt < 1:372398/141‑1 (MQ=255) tttCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGt < 2:342022/141‑1 (MQ=255) tcttATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTActtctt < 3:407604/141‑1 (MQ=255) ttATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTa < 2:442963/141‑1 (MQ=255) ttATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTAGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAAAATGCCTAGTACCATTAATACATACATTATCGGATGTACTTCTTTa < 2:227168/141‑1 (MQ=255) aTTTTATCCTTACTTCAAAG‑AAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGt < 3:270325/141‑1 (MQ=255) ttttATCCTTACTACAAAGAAAAAAGCGACTACCAATGATTGGTAGTCGTTTTTTGTGTCTGGCTTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGt > 2:922532/1‑141 (MQ=255) tttATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAACGAATAGTACCATTACTACATACATTATCGGACGTACTTATTTATGtt > 2:1036659/1‑141 (MQ=255) ttATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGttt < 2:179059/141‑1 (MQ=255) tATCCTCACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCAGATCATCTTCTTTATGtttt > 1:332609/1‑141 (MQ=255) aTCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGttttt < 1:211058/141‑1 (MQ=255) cTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTag > 1:999136/1‑141 (MQ=255) ttACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTaga > 2:284068/1‑141 (MQ=255) ttACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTaga > 1:660536/1‑141 (MQ=255) tACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTagag > 1:271770/1‑141 (MQ=255) aCTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAgaga > 2:1062404/1‑141 (MQ=255) aCTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAgaga > 2:326698/1‑141 (MQ=255) aaaGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGc < 1:410136/141‑1 (MQ=255) aaaGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGc > 1:1077095/1‑141 (MQ=255) aGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCAt > 1:703687/1‑141 (MQ=255) aGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCAt < 4:280748/141‑1 (MQ=255) gAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCAtt < 4:385297/141‑1 (MQ=255) aaaaaaCGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGt < 2:445094/141‑1 (MQ=255) aaaaaCGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTa > 3:368362/1‑141 (MQ=255) aaaaaCGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTa > 3:17465/1‑141 (MQ=255) aaaaCGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTaa > 2:993986/1‑141 (MQ=255) aaaCGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAAt < 2:726372/141‑1 (MQ=255) aaCGACTGCCAATGATTGGTAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAAtt > 1:48361/1‑141 (MQ=255) aCGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGCTAAGCATTGTAATTg > 4:339303/1‑141 (MQ=255) aCGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTg > 1:1080401/1‑141 (MQ=255) aCGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTg < 4:173222/141‑1 (MQ=255) tACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAg > 1:905071/1‑141 (MQ=255) ccAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTATCCAAAAACAAATATTAATTACATAATTAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGaa < 1:99231/141‑1 (MQ=255) cAATGATTGGTAGTCGTTTTTTTTGTCTGGTTTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGaaa > 2:136547/1‑141 (MQ=255) cATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATGAATACATATATGATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAaaataa > 1:214198/1‑141 (MQ=255) | TATAATATAGAAGAAACACGTCCAACAATTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAAAATAA > USA300TCH1516_ALE/2372275‑2372553 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |