Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 124,550 G→A 6.1% D229N (GAT→AAT)  norG → HTH‑type transcriptional regulator NorG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE124,5500GA 6.1% 397.0 / 11.2 132D229N (GAT→AAT) norGHTH‑type transcriptional regulator NorG
Reads supporting (aligned to +/- strand):  ref base G (62/61);  new base A (3/5);  total (65/67)
Fisher's exact test for biased strand distribution p-value = 7.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.85e-01

Only 100 of 132 total aligned reads displayed.

TCAACTTTTATCTATTGGGTTTTTAGGTCAAGATGCCATAATAATTTCGAATACACCATCATATATTCACTCTACAAATGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTTAATAACCCGACAGGTCGTTCTTTAACGAATGAGCAAAAGAAAAATATAATTACTTATAGCGAAAGACATAATATT  >  USA300TCH1516_ALE/124411‑124687
                                                                                                                                           |                                                                                                                                         
 cAACTTTTATCTATTGGGTTTTTAGGTCAAGATGCCATAATAATTTCGAATACACCATCATATATTCACTCTACAAATGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGAt                                                                                                                                         <  1:98502/141‑1 (MQ=255)
 cAAATTTTATCTATTGGGTTTTTAGGTCAAGATGCCATAATAATTTCGAATACACCATCATATATTCACTCTACAAATGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGAt                                                                                                                                         <  4:209792/141‑1 (MQ=255)
   aCTTTTATCTATTGGGTTTTTAGGTCAAGATGCCATAATAATTTCGAATACACCATCATATATTCACTCTACAAATGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATAc                                                                                                                                       >  2:1050343/1‑141 (MQ=255)
    cTTTTATCTATTGGGTTTTTAGGTCAAGATGCCATAATAATTTCGAATACACCATCATATATTCACTCTACAAATGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATAcc                                                                                                                                      <  2:531871/141‑1 (MQ=255)
    cTTTTATCTATTGGGTTTTTAGGTCAAGATGCCATAATAATTTCGAATACACCATCATATATTCACTCTACAAATGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATAcc                                                                                                                                      <  4:266842/141‑1 (MQ=255)
       ttATCTATTGGGTTTTTAGGTCAAGATGCCATAATAATTTCGAATACACCATCATATATTCACTCTACAAATGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTTTAATCAAATTAATGAAATTGATACcatc                                                                                                                                   <  2:541762/141‑1 (MQ=255)
          tctcTTGGGTTTTTAGCCCAAGACGCCATAATAATTACGAATCCACCATCTTATATTATAAATACTCTTGCTTTTTAGCAATTTAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCAtt                                                                                                                                <  1:541986/141‑1 (MQ=255)
          tCTATTGGGTTTTTAGGTCAAGATGCCATAATAATTTCGAATACACCATCATATATTCACTCTACAAATGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTCCACTCCACTTCCTGAAATTGATCCcaccctc                                                                                                                                >  2:287822/1‑136 (MQ=255)
          ccTATTGGGTTTTTAGGTCAAGATGCCATAATAATTTCGAATACACCATCATATATTCACTCTACAAATGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCAtt                                                                                                                                <  1:1001070/140‑1 (MQ=255)
           cTATTGGGTTTTTAGGTCAAGATGCCATAATAATTTCGAATACACCATCATATATTCACTCTACAAATGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTg                                                                                                                               <  1:760513/141‑1 (MQ=255)
           cTATTGGGTTTTTAGGTCAAGATGCCATAATAATTTCGAATACACCATCATATATTCACTCTACAAATGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTg                                                                                                                               >  4:396600/1‑141 (MQ=255)
           cTATTGGGTTTTTAGGTCAAGATGCCATAATAATTTAGAATAAACAATCATATATTCAATCTACAAATGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACaatcatag                                                                                                                               >  2:322261/1‑139 (MQ=255)
              ttGGGTTTTTAGGTCAAGATGCCATAATAATTTCGAATACACCATCATATATTCACTCTACAAATGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATa                                                                                                                            >  1:421037/1‑141 (MQ=255)
                gggTTTTTAGGTCAAGATGCCATAATAATTTCGAATACACCATCATATATTCACTCTACAAATGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGa                                                                                                                          <  2:806134/141‑1 (MQ=255)
                     tttAGGTCAAGATGCCATAATAATTTCGAATACACCATCATATATTCACTCTACAAATGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATttat                                                                                                                     >  3:92335/1‑141 (MQ=255)
                     tttAGGTCAAGATGCCATAATAATTTCGAATACACCATCATATATTCACTCTACAAATGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATttat                                                                                                                     >  1:583465/1‑141 (MQ=255)
                     tttAGGTCAAGATGCCATAATAATTTCGAATACACCATCATATATTCACTCTACAAATGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATttat                                                                                                                     <  2:402398/141‑1 (MQ=255)
                      ttAGGTCAAGATGCCATAATAATTTCGAATACACCATCATATATTCACTCTACAAATGTTTTTGAGCAATTGAATTTTGGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATttatt                                                                                                                    <  1:716187/141‑1 (MQ=255)
                      ttAGGTCAAGATGCCATAATAATTTCGAATACACCATCATATATTCACTCTACAAATGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATttatt                                                                                                                    <  3:292310/141‑1 (MQ=255)
                      ttAGGTCAAGATGCCATAATAATTTCGAATACACCATCATATATTCACTCTACAAATGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATttatt                                                                                                                    <  2:991256/141‑1 (MQ=255)
                        aGGTCAAGATGCCATAATAATTTCGAATACACCATCATATATTCACTCTACAAATGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTAATACCATCATTGATAGATTTATTaa                                                                                                                  >  4:439507/1‑141 (MQ=255)
                         ggTCAAGATGCCATAATAATTTCGAATACACCATCATATATTCACTCTACAAATGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAAt                                                                                                                 >  4:311285/1‑141 (MQ=255)
                         ggTCAAGATACTATAATAATTTCGAATACACCATCATATATTCACTCTACAAATGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATAAAATTGATACCATCATTGATAGATTTATTAAt                                                                                                                 >  2:685249/1‑141 (MQ=255)
                          gTCAAGATGCCATAATAATTTCGAATACACCATCATATATTCACTCTACAAATGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAAtt                                                                                                                >  1:460368/1‑141 (MQ=255)
                           tCAAGATGCCATAATAATTTCGAATACACCATCATATATTCACTCTACAAATGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAAttt                                                                                                               <  1:677994/141‑1 (MQ=255)
                            cAAGATGCCATAATAATTTCGAATACACCATCATATATTCACTCTACAAATGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAAtttt                                                                                                              >  1:390542/1‑141 (MQ=255)
                             aaGATGCCATAATAATTTCGAATACACCATCATATATTCACTCTACAAATGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTa                                                                                                             <  1:856859/141‑1 (MQ=255)
                              aGATGCCATAATAATTTCGAATACACCATCATATATTCACTCTACAAATGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTaa                                                                                                            <  1:154605/141‑1 (MQ=255)
                                   ccATAATAATTTCGAATACACCATCATATATTCACTCTACAAATGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATa                                                                                                       <  2:797549/141‑1 (MQ=255)
                                   ccATAATAATTTCGAATACACCATCATATATTCACTCTACAAATGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATa                                                                                                       >  3:296485/1‑141 (MQ=255)
                                           atTTCGAATACACCATCATATATTCACTCTACAAATGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGAtt                                                                                               >  1:1009007/1‑141 (MQ=255)
                                              tCGAATACACCATCATATATTCACTCTACAAATGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTtat                                                                                            >  1:448248/1‑141 (MQ=255)
                                                  aTACACCATCATATATTCACTCTACAAATGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAg                                                                                        <  3:190331/141‑1 (MQ=255)
                                                      acCATCATATATTCACTCTACAAATGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAAcc                                                                                    <  2:925996/141‑1 (MQ=255)
                                                      acCATCATATATTCACTCTACAAATGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAAcc                                                                                    <  2:282035/141‑1 (MQ=255)
                                                      acCATCATATATTCACTCTACAAATGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTAATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAAcc                                                                                    <  1:959538/141‑1 (MQ=255)
                                                       ccATCATATATTCACTCTACAAATGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCt                                                                                   >  1:945900/1‑141 (MQ=255)
                                                         atcatATATTCACTCTACAAATGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAg                                                                                 >  1:413203/1‑141 (MQ=255)
                                                          tcatATATTCACTCTACAAATGTTTTTGAGCAATTGACTTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAAGTGATACCATCATTGATAGATTTANTAANTTTANAAATAAAGCGNTTTATATAGAACCTAgg                                                                                <  1:705972/141‑1 (MQ=255)
                                                              atatTCACTCTACAAATGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTta                                                                            <  1:457382/141‑1 (MQ=255)
                                                               tatTCACTCTACAAATGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTtaa                                                                           >  1:675784/1‑141 (MQ=255)
                                                               tatTCACTCTACAAATGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTtaa                                                                           >  4:298089/1‑141 (MQ=255)
                                                                 ttCACTCTACAAATGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTtaata                                                                         >  3:59045/1‑141 (MQ=255)
                                                                 ttCACTCTACAAATGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTAATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTtaata                                                                         >  4:177937/1‑141 (MQ=255)
                                                                 ttCACTCTACAAATGTTTTTGAGAAATTGGACTTTTGTCATATTTATGTTCCTTAGAATCAAATTAATGAGATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTAATATAGAACATATGTTgaata                                                                         >  4:128910/1‑141 (MQ=255)
                                                                  tCACTCTACAAATGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTtaataa                                                                        <  2:580491/141‑1 (MQ=255)
                                                                   cACTCTACAAATGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTTAATAAc                                                                       >  2:866889/1‑141 (MQ=255)
                                                                     ctctACAAATGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTTAATAAccc                                                                     >  2:206569/1‑141 (MQ=255)
                                                                     ctctACAAATGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTAATACCATCATTGATAGa                                                                                                                          >  2:258078/1‑88 (MQ=255)
                                                                     ctctACAAATGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTAATACCATCATTGATAGa                                                                                                                          <  1:258078/88‑1 (MQ=255)
                                                                      tctACAAATGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTTAATAACCCg                                                                    >  1:929832/1‑141 (MQ=255)
                                                                       ctACAAATGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTTAATAACCCGa                                                                   <  2:925571/141‑1 (MQ=255)
                                                                       ctACAAATGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTTAATAACCCGa                                                                   >  4:83608/1‑141 (MQ=255)
                                                                           aaaTGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACGATCGTTGATAGATTGATGAATTTTAAAAATAAAGCGATTTATATATAACCTAGGTTTAATAACCCGACAgg                                                               >  1:918240/1‑141 (MQ=255)
                                                                           aaaTGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTTAATAACCCGACAgg                                                               <  2:118655/141‑1 (MQ=255)
                                                                           aaaTGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTTAATAACCCGACAgg                                                               <  2:118724/141‑1 (MQ=255)
                                                                             aTGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTTAATAACCCGACAGGTc                                                             <  2:618632/141‑1 (MQ=255)
                                                                             aTGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTTAATAACCCGACAGGTc                                                             >  4:296135/1‑141 (MQ=255)
                                                                              tGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTTAATAACCCGACAGGTCg                                                            <  3:207556/141‑1 (MQ=255)
                                                                              tGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTTAATAACCCGACAGGTCg                                                            <  3:353731/141‑1 (MQ=255)
                                                                              tGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTTAATAACCCGACAGGTCg                                                            <  1:676186/141‑1 (MQ=255)
                                                                               gTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTTAATAACCCGACAGGTCGt                                                           >  1:801913/1‑141 (MQ=255)
                                                                                 ttttGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTAATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTTAATAACCCGACAGGTCGTTc                                                         <  3:128060/141‑1 (MQ=255)
                                                                                 ttttGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTAATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTTAATAACCCGACAGGTCGTTc                                                         <  3:208643/141‑1 (MQ=255)
                                                                                  tttGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTTAATAACCCGACAGGTCGTTCt                                                        <  1:240812/141‑1 (MQ=255)
                                                                                  tttGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTTAATAACCCGACAGGTCGTTCt                                                        <  3:15302/141‑1 (MQ=255)
                                                                                  tttGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCCATTTATATAGAACCTAGGTTTAATAACCCGACAGGTCGTTCt                                                        <  3:215220/141‑1 (MQ=255)
                                                                                    tGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTTAATAACCCGACAGGTCGTTCttt                                                      <  2:21117/141‑1 (MQ=255)
                                                                                    tGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTTAATAACCCGACAGGTCGTTCttt                                                      <  3:152526/141‑1 (MQ=255)
                                                                                     gAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTTAATAACCCGACAGGTCGTTCTTTa                                                     <  2:87378/141‑1 (MQ=255)
                                                                                     gAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTTAATAACCCGACAGGTCGTTCTTTa                                                     >  1:729828/1‑141 (MQ=255)
                                                                                     gAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTAATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTTAATAACCCGACAGGTCGTTCTTTa                                                     <  3:167924/141‑1 (MQ=255)
                                                                                           ttGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTTAATAACCCGACAGGTCGTTCTTTAACGAAt                                               >  4:25106/1‑141 (MQ=255)
                                                                                               aTTTTAGACATATTGATGTTCCTTATAATCAAATTAATTACATGCAGACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTTAATAACCCGACAGGTCGTTCTTTAACGAATGAGc                                           <  1:826862/141‑1 (MQ=255)
                                                                                               aTTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTTAATAACCCGACAGGTCGTTCTTTAACGAATGAGc                                           <  3:454767/141‑1 (MQ=255)
                                                                                               aTTTTAGACATATTGATGTTCCTTATAATAAAATTAATGAAATTGATACCATAAAAAAAAAAAAAAAAAAATTTAAAAATAAAGCGATTTATATACCACCTAGGTGTAATAACCCGACAGGGCGTTCTTTAACGaaaaaaa                                           <  4:233381/141‑6 (MQ=255)
                                                                                                ttttAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTCATTTTACAAATAAAGCGATTTATATAGAACCTAGGTTTAATAACCCGACAGGTCGTTCTTTAACGAATGAGCa                                          <  3:409867/141‑1 (MQ=255)
                                                                                                   tAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTTAATAACCCGACAGGTCGTTCTTTAACGAATGAGCaaaa                                       >  1:963746/1‑141 (MQ=255)
                                                                                                    aGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTTAATAACCCGACAGGTCGTTCTTTAACGAATGAGCAAAAg                                      >  3:203171/1‑141 (MQ=255)
                                                                                                    aGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTTAATAACCCGACAGGTCGTTCTTTAACGAATGAGCAAAAg                                      >  1:590122/1‑141 (MQ=255)
                                                                                                         tatTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTTAATAACCCGACAGGTCGTTCTTTAACGAATGAGCAAAAGaaaaa                                 <  3:83608/141‑1 (MQ=255)
                                                                                                             gATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTTAATAACCCGACAGGTCGTTCTTTAACGAATGAGCAAACGAAAAAtata                             >  2:711006/1‑141 (MQ=255)
                                                                                                             gATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTTAATAACCCGACAGGTCGTNCTTTAACGAATGNGCAAAAGAAAAAtata                             <  1:707633/141‑1 (MQ=255)
                                                                                                              aTGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTTAATAACCCGACAGGTCGTTCTTTAACGAATGAGCAAAAGAAAAATATaa                            <  1:888172/141‑1 (MQ=255)
                                                                                                                   ccTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTTAATAACCCGACAGGTCGTTCTTTAACGAATGAGCAAAAGAAAAATATAATTACt                       >  2:892601/1‑141 (MQ=255)
                                                                                                                       ataataAAATTAATGAAAGTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTTAATAACCCGACAGGTCGTTCTTTAACGAATAAAAAAAAAAAAAATATAATTACTtata                   <  2:540016/141‑1 (MQ=255)
                                                                                                                        taataaAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATGAAGCGATTTATATAGAACCTCGGTTTAATAACCCGACAGGTCGTTCTTTAACGAATGAGAAAAAGAAAAATATAATTACTTATAg                  <  4:224600/141‑1 (MQ=255)
                                                                                                                          aTCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTTAATAACCCGACAGGTCGTTCTTTAACGAATGAGCAAAAGAAAAATATAATTACTTATAGCg                >  1:147736/1‑141 (MQ=255)
                                                                                                                            cAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTTAATAACCCGACAGGTCGTTCTTTAACGAATGAGCAAAAGAAAAATATAATTACTTATAGCGaa              <  1:54210/141‑1 (MQ=255)
                                                                                                                             aaaTTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTTAATAACCCGACAGGTCGTTCTTTAACGAATGAGCAAAAGAAAAATATAATTACTTATAGCGaaa             >  2:443236/1‑141 (MQ=255)
                                                                                                                               aTTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTTAATAACCCGACAGGTCGTTCTTTAACGAATGAGCAAAAGAAAAATATAATTACTTATAGCGAAAGa           <  1:524348/141‑1 (MQ=255)
                                                                                                                                ttAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTTAATAACCCGACAGGTCGTTCTTTAACGAATGAGCAAAAGAAAAATATAATTACTTATAGCGAAAGAc          >  1:87683/1‑141 (MQ=255)
                                                                                                                                ttAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTTAATAACCCGACAGGTCGTTCTTTAACGAAAGAGAAAAAGAAAAATATAATTACTTATAGCGAAAGAc          <  2:752541/141‑1 (MQ=255)
                                                                                                                                  aaTGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGTACCTAGGTTTAATAACCCTATTGGTCGTTCTTTATCGAATGAGCAAAAGAAAAATATAATTACTTATAGCGAAAGACat        >  1:590458/1‑141 (MQ=255)
                                                                                                                                     gAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTTAATAACCCGACAGGTCGTTCTTTAACGAATGAGCAAAAGAAAAATATAATTACTTATAGCGAAAGACataat     >  3:328453/1‑141 (MQ=255)
                                                                                                                                     gAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTTAATAACCCGACAGGTCGTTCTTTAACGAATGAGCAAAAGAAAAATATAATTACTTATAGCGAAAGACataat     <  1:316824/141‑1 (MQ=255)
                                                                                                                                      aaaTTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATCGAACCTAGGTTTAATAACCCGACAGGTCGTTCTTTAACGAATGAGCAAAAGAAAAATATAATTACTTATAGCGAAAGACataata    >  3:8043/1‑141 (MQ=255)
                                                                                                                                      aaaTTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTTAATAACCCGACAGGTCGTTCTTTAACGAATGAGCAAAAGAAAAATATAATTACTTATAGCGAAAGACataata    >  3:8229/1‑141 (MQ=255)
                                                                                                                                       aaTTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTTAATAACCCGACAGGTCGTTCTTTAACGAATGAGCAAAAGAAAAATATAATTACTTATAGCGAAAGACATAatat   >  4:105017/1‑141 (MQ=255)
                                                                                                                                        aTTGATACCATAATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTTAATAACCCGACAGGTCGTTCTTTAACGAAAAAAAAAAAAAAAAATATAATTACTTATAGCGAAAGACATAATAtt  <  2:489493/141‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                         
TCAACTTTTATCTATTGGGTTTTTAGGTCAAGATGCCATAATAATTTCGAATACACCATCATATATTCACTCTACAAATGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTTAATAACCCGACAGGTCGTTCTTTAACGAATGAGCAAAAGAAAAATATAATTACTTATAGCGAAAGACATAATATT  >  USA300TCH1516_ALE/124411‑124687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: