BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	2,379,107	A→C	5.1%	V121G (GTT→GGT)	USA300HOU_RS12220 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,379,107	0	A	C	5.1%	480.6 / 14.3	156	V121G (GTT→GGT)	USA300HOU_RS12220	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (76/72); new base C (4/4); total (80/76)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

Only 100 of 157 total aligned reads displayed.

TATGTAACGAGTGGCACTGATTGTAATAATTTCTTACCTAAATATTTAAAATCGAATTGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCATACCTACTGATCCACGCATGTGATTATCATATTTTCTAAAAAACCAGACAGCAATC  >  USA300TCH1516_ALE/2378969‑2379247

 atgtaACGAGTGGCACTGATTGTAATAATTTCTTACCTAAATATTTAAAATCGAATTGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACaa                                                                                                                                           <  1:130992/141‑1 (MQ=255)

 atgtaACGAGTGGCACTGATTGTAATAATTTCTTACCTAAATATTTAAAATCGAATTGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACaa                                                                                                                                           <  1:395168/141‑1 (MQ=255)

   gtaACGAGTGGCACTGATTGTAATAATTTCTTACCTAAATATTTAAAATCGAATTGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACaaaa                                                                                                                                         <  1:241291/141‑1 (MQ=255)

    taACGAGTGGCACTGATTGTAATAATTTCTTACCTAAATATTTAAAATCGAATTGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAaaat                                                                                                                                        >  2:492555/1‑141 (MQ=255)

     aaCGAGTGGCACTGATTGTAATAATTTCTTACCTAAATATTTAAAATCGAATTGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAaaata                                                                                                                                       >  1:413243/1‑141 (MQ=255)

      aCGAGTGGCACTGATTGTAATAATTTCTTACCTACATATTGAAAATCGAATTGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAaaataa                                                                                                                                      >  4:26489/1‑141 (MQ=255)

      aCGAGTGGCACTGATTGTAATAATTTCTTACCTAAATATTTAAAATCGAATTGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAaaataa                                                                                                                                      >  2:172779/1‑141 (MQ=255)

      aCGAGTGGCACTGATTGTAATAATTTATTACCTAAATATTTAAAATCGAATTGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACANCACAAAGGCACCACCTATANCAGCCATAACAaaataa                                                                                                                                      <  3:285140/141‑1 (MQ=255)

           tGGCACTGATTGTAATAATTTCTTACCTAAATATTTAAAATCGAATTGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAg                                                                                                                                 <  2:899566/141‑1 (MQ=255)

            ggCACTGATTGTAATAATTTCTTACCTAAATATTTAAAATCGAATTGATCACTACCTTCACTAATGTACCTTCCTTCAAATAAATTTTCTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGt                                                                                                                                >  1:1028646/1‑141 (MQ=255)

            ggCACTGATTGTAATAATTTCTTACCTAAATATTTAAAATCGAATTGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGt                                                                                                                                >  2:438932/1‑141 (MQ=255)

             gCACTGATTGTAATAATTTCTTACCTAAATATTTAAAATCGAATTGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTc                                                                                                                               <  1:77333/141‑1 (MQ=255)

              cACTGATTGTAATAATTTCTTACCTAAATATTTAAAATCGAATTGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTcc                                                                                                                              >  3:77290/1‑141 (MQ=255)

               aCTGATTGTAATAATTTCTTACCTAAATATTTAAAATCGAATTGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTccc                                                                                                                             <  1:181829/141‑1 (MQ=255)

                cTGATTGTAATAATTTCTTACCTAAATATTTAAAATTGAATTGATAACTACCTTCACTAAATTAGCTACCTACAAAGTAAGTAATTTCAAACATCACAAAGGCACAACCTATATAAGCCATAACAAAATAAATAAGTCACg                                                                                                                            >  2:84394/1‑141 (MQ=255)

                cTGATTGTAATAATTTCTTACCTAAATATTTAAAATCGAATTGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTccca                                                                                                                            <  2:241309/141‑2 (MQ=255)

                cTGATTGTAATAATTTCTTACCTAAATATTTAAAATCGAATTGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTccca                                                                                                                            <  2:241267/141‑2 (MQ=255)

                   actGTAATAATTTCTTACCTAAATATTTAAAATCGAATTGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATACCAAAATAAATAAGCCCCGttt                                                                                                                         >  3:144926/3‑141 (MQ=255)

                      gTAATAATTTCTTACCTAAATATTTAAAATCGAATTGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAg                                                                                                                      >  1:997833/1‑141 (MQ=255)

                       taataaTTTCTTACCTAAATATTTAAAATCGAATTGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAgg                                                                                                                     >  1:695262/1‑141 (MQ=255)

                       taataaTTTCTTACCTAAATATTTAAAATCGAATTGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAgg                                                                                                                     >  2:722361/1‑141 (MQ=255)

                        aataatTTCTTACCTAAATATTTAAAATCGAATTGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGc                                                                                                                    >  1:429513/1‑141 (MQ=255)

                         ataatTTCTTACCTAAATATTTAAAATCGAATTGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGcc                                                                                                                   <  1:63965/141‑1 (MQ=255)

                         ataatTTCTTACCTAAATATTTAAAATCGAATTGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGcc                                                                                                                   >  2:142280/1‑141 (MQ=255)

                           aatTTCTTACCTAAATATTTAAAATCGAATTGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGccct                                                                                                                 >  2:45726/1‑139 (MQ=255)

                            atTTCTTACCTAAATATTTAAAATCGAATTGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCata                                                                                                                >  2:498402/1‑141 (MQ=255)

                             tttCTTACCTAAATATTTAAAATCGAATTGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATACCAAAATAAATAAGCCCCGTTTTAGGCCatat                                                                                                               <  2:251397/141‑1 (MQ=255)

                             tttCTTACCTAAATATTTAAAATCGAATTGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCatat                                                                                                               <  4:160110/141‑1 (MQ=255)

                               tCTTACCTAAATATTTAAAATCGAATTGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCa                                                                                                             >  3:321123/1‑141 (MQ=255)

                                   aCCTAAATATTTAAAATCGAATTGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTc                                                                                                         >  1:285316/1‑141 (MQ=255)

                                      tAAATATTTAAAATCGAATTGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAAt                                                                                                      <  2:405831/141‑1 (MQ=255)

                                       aaaTATTTAAAATCGAATTGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAAtt                                                                                                     <  2:825961/141‑1 (MQ=255)

                                        aaTATTTAAAATCGAATTGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTa                                                                                                    <  4:237282/141‑1 (MQ=255)

                                            tttAAAATCGAATTGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTgg                                                                                                <  2:405081/141‑1 (MQ=255)

                                            tttAAAATCGAATTGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTgg                                                                                                <  2:595232/141‑1 (MQ=255)

                                             ttAAAATCGAATTGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGa                                                                                               <  1:331726/141‑1 (MQ=255)

                                               aaaaTCGAATTGATCACTAACTTCACTAAAGTAGATATCTACAAAGTAAGTAATTCCAAATATCACAAAGGCACCACCTATATAAGCCATAAAAAAATAAATAAGTCCCGTTTTAGGCAATAACACTTAAATTAGTGGata                                                                                             >  4:157720/1‑141 (MQ=255)

                                                aaaTCGAATTGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGatac                                                                                            >  1:670130/1‑140 (MQ=255)

                                                   tCGAATTGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCa                                                                                         <  4:313541/141‑1 (MQ=255)

                                                    cGAATTGATCACTGCCATCACGAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAATTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCaa                                                                                        <  4:380866/141‑1 (MQ=255)

                                                    cGAATTGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCaa                                                                                        <  1:281563/141‑1 (MQ=255)

                                                    cGAATTGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCaa                                                                                        <  1:235665/141‑1 (MQ=255)

                                                    cGAATTGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCaa                                                                                        <  1:155986/141‑1 (MQ=255)

                                                     gAATTGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTACTTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCGCTTCAATTAGTGGATATGCaaa                                                                                       >  3:379607/1‑141 (MQ=255)

                                                      aaTTGATCACTACACTCACTAAAGTATCTACCTACAAAGTAAGTAATTCCAAACACCACAAAGGCACCACCTATATCAGCCATACCAAAATAAATAAGCCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAAc                                                                                      <  2:859783/141‑1 (MQ=255)

                                                         tGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAAt                                                                                   <  3:125866/141‑1 (MQ=255)

                                                          gATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATc                                                                                  <  2:104720/141‑1 (MQ=255)

                                                           aTCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATcc                                                                                 >  1:1043187/1‑141 (MQ=255)

                                                            tCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCa                                                                                <  2:991791/141‑1 (MQ=255)

                                                            tCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCa                                                                                >  2:306934/1‑141 (MQ=255)

                                                            tCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCa                                                                                <  3:443519/141‑1 (MQ=255)

                                                                  ccTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTc                                                                          <  3:48536/141‑1 (MQ=255)

                                                                     tCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATa                                                                       >  2:699896/1‑141 (MQ=255)

                                                                      cACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGata                                                                                             >  2:213394/1‑118 (MQ=255)

                                                                       aCTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATACCAAAATAAATAAGCCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGc                                                                     >  1:233590/1‑141 (MQ=255)

                                                                       aCTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATACCAAAATAAATAAGCCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGc                                                                     >  3:412697/1‑141 (MQ=255)

                                                                       aCTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGc                                                                     <  2:619329/141‑1 (MQ=255)

                                                                            aGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAg                                                                <  2:515986/141‑1 (MQ=255)

                                                                            aGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAg                                                                <  2:334037/141‑1 (MQ=255)

                                                                            aGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAg                                                                >  2:865936/1‑141 (MQ=255)

                                                                            aGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAg                                                                >  2:962712/1‑141 (MQ=255)

                                                                             gTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACGAGt                                                               >  1:143641/1‑141 (MQ=255)

                                                                               aGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGtca                                                             <  1:970348/141‑1 (MQ=255)

                                                                                gCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGtcat                                                            >  2:490591/1‑141 (MQ=255)

                                                                                gCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGtcat                                                            <  1:533107/141‑1 (MQ=255)

                                                                                gCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGtcat                                                            >  3:191957/1‑141 (MQ=255)

                                                                                          aagtaagtAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCATACCTACt                                                  >  1:223390/1‑141 (MQ=255)

                                                                                           agtaagtaATTCCAAACATCACAAAGGCACCACCTATATCAGCCATACCAAAATAAATAAGCCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCATACCTACTg                                                 >  2:204056/1‑141 (MQ=255)

                                                                                            gtaagtaaTTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCATACCTACTGa                                                <  1:426240/141‑1 (MQ=255)

                                                                                            gtaagtaaTTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCATACCTACTGa                                                <  4:4907/141‑1 (MQ=255)

                                                                                               agtaaTTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCATACCTACTGATcc                                             >  1:884066/1‑141 (MQ=255)

                                                                                               agtaaTTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCATACCTACTGATcc                                             >  1:847106/1‑141 (MQ=255)

                                                                                                gtaaTTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCATACCTACTGATCCa                                            >  4:75566/1‑141 (MQ=255)

                                                                                                gtaaTTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCATACCTACTGATCCa                                            >  4:344264/1‑141 (MQ=255)

                                                                                                gtaaTTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCATACCTACTGATCCa                                            >  2:145331/1‑141 (MQ=255)

                                                                                                    ttCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCATACCTACTGATCCACGCa                                        <  2:7756/141‑1 (MQ=255)

                                                                                                    ttCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCATACCTACTGATCCACGCa                                        >  2:1050157/1‑141 (MQ=255)

                                                                                                         aaCATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCATACCTACTGATCCACGCATGTGa                                   >  2:74004/1‑141 (MQ=255)

                                                                                                         aaCATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCATACCTACTGATCCACGCATGTGa                                   >  2:912665/1‑141 (MQ=255)

                                                                                                          aCATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCATACCTACTGATCCACGCATGTGAt                                  >  2:349394/1‑141 (MQ=255)

                                                                                                           cATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCATACCTACTGATCCACGCATGTGAtt                                 >  3:191979/1‑141 (MQ=255)

                                                                                                            aTCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCATACCTACTGATCCACGCATGTGATTa                                >  1:125177/1‑141 (MQ=255)

                                                                                                             tCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCATACCTACTGATCCACGCATGTGATTAt                               >  1:147475/1‑141 (MQ=255)

                                                                                                             tCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCATACCTACTGATCCACGCATGTGATTAt                               >  3:272514/1‑141 (MQ=255)

                                                                                                             tCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCAGATCACGTCAATTAGGGGAGATGCAAACAATCCAATATTCATATCACCAGTCATCATACTTACTGATCCACGCATGTGATTAt                               >  1:758920/1‑141 (MQ=255)

                                                                                                              cgcgaaGGCACCGCCTATATCAGCCATAACAAAACAAATAAGTCCCCGTTTAGGCCATATCACTTCAAATAGTGGATATGCAAACAAGCCAATACGCATCGCACCAGGCATCATACCTTCTGATCCACGCATGTGATTATc                              <  4:352546/137‑1 (MQ=255)

                                                                                                               acaAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCTATATTCATAGCACCAGTCATCATACCTACTGATCCACGCATGTGATTATCa                             <  2:1054170/141‑1 (MQ=255)

                                                                                                               acaAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCATACCTACTGATCCACGCATGTGATTATCa                             >  1:240114/1‑141 (MQ=255)

                                                                                                                  aaGGCACCACCTATATCAGCCATAACAAAATCAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCCTAACAATCGGTGCATGCATGTGATTATCatat                          <  4:457745/141‑1 (MQ=255)

                                                                                                                  aaGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCATACCTACTGATCCACGCATGTGATTATCatat                          <  1:840973/141‑1 (MQ=255)

                                                                                                                      caccacCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCATACCTACTGATCCACGCATGTGATTATCATATTTTc                      <  1:530842/141‑1 (MQ=255)

                                                                                                                        ccaccTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCATACCTACTGATCCACGCATGTGATTATCATATTTTCTa                    >  1:695942/1‑141 (MQ=255)

                                                                                                                         caccTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCATACCTACTGATCCACGCATGTGATTATCATATTTTCTaa                   <  2:526005/141‑1 (MQ=255)

                                                                                                                            cTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCATGCCTACTGATCCACGCATGTGATTATCATATTTTCTaaaaa                >  4:401924/1‑141 (MQ=255)

                                                                                                                                  cAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCATACCTACTGATCCACGCATGTGATTATCATATTTTCTAAAAAACCAGa          >  1:813371/1‑141 (MQ=255)

                                                                                                                                  cAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCATACCTACTGATCCACGCATGTGATTATCATATTTTCTAAAAAACCAGa          >  3:222281/1‑141 (MQ=255)

                                                                                                                                   aGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCATACCTACTGATCCACGCATGTGATTATCATATTTTCTAAAAAACCAGAc         >  1:633788/1‑141 (MQ=255)

                                                                                                                                    gCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTGCAATTAGTGGATATTCAAACAATCCAATATTCATAGCACCAGTCATCATACCTACTGATCCACGCATGTGATTATCATATTTTCTAAAAAACCAGACa        <  1:99248/141‑1 (MQ=255)

                                                                                                                                       aTAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCATACCTACTGATCCACGCATGTGATTATCATATTTTCTAAAAAACCAGACAGCa     >  3:447145/1‑141 (MQ=255)

                                                                                                                                          aCAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCATACCTACTGATCCACGCATGTGATTATCATATTTTCTAAAAAACCAGACAGCAATc  >  2:355656/1‑141 (MQ=255)

TATGTAACGAGTGGCACTGATTGTAATAATTTCTTACCTAAATATTTAAAATCGAATTGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCATACCTACTGATCCACGCATGTGATTATCATATTTTCTAAAAAACCAGACAGCAATC  >  USA300TCH1516_ALE/2378969‑2379247

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`