BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	2,379,394	T→C	6.7%	K25K (AAA→AAG)	USA300HOU_RS12220 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,379,394	0	T	C	6.7%	386.9 / 13.6	134	K25K (AAA→AAG)	USA300HOU_RS12220	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (54/71); new base C (2/7); total (56/78)
Fisher's exact test for biased strand distribution p-value = 3.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.57e-01

Only 100 of 143 total aligned reads displayed.

GCCACAATACCATATATAATCATTAAAATTGGTAAAATGGAGAATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGTTATCCCTTCTTTCAATAATCTTCATAATTTATAACTTTAACATACTCCACAGATATTTTAGA  >  USA300TCH1516_ALE/2379254‑2379533

gCCACAATACCATATATAATCATTAAAATTGGTAAAATGGAGAATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCt                                                                                                                                             <  1:1045862/141‑1 (MQ=255)

gCCACAATACCATATATAATCATTAAAATTGGTAAAATGGAGAATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCt                                                                                                                                             >  2:677409/1‑141 (MQ=255)

gCCACAATACCATATATAATCATTAAAATTGGTAAAATGGAGAATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCt                                                                                                                                             >  3:323107/1‑141 (MQ=255)

  cacaATACCATATATAATCATTAAAATTGGTAAAATGGAGAATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCttt                                                                                                                                           >  4:370272/1‑141 (MQ=255)

   acaATACCATATATAATCATTAAAATTGGTAAAATGGAGAATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCtttt                                                                                                                                          >  3:106527/1‑141 (MQ=255)

     aaTACCATATATAATCATTAAAATTGGTAAAATGGAGAATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTtat                                                                                                                                        <  1:349394/141‑1 (MQ=255)

     aaTACCATATATAATCATTAAAATTGGTAAAATGGAGAATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTtat                                                                                                                                        >  2:285819/1‑141 (MQ=255)

          cATATATAATCATTAAAATTGGTAAAATGGAGAATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAAt                                                                                                                                   >  4:170405/1‑141 (MQ=255)

          cATATATAATCATTAAAATTGGTAAAATGGAGAATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAAt                                                                                                                                   >  3:339057/1‑141 (MQ=255)

           atatatAATCATTAAAATTGGTAAAATGGAGAATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAAtt                                                                                                                                  >  1:1052525/1‑141 (MQ=255)

            tatataATCATTAAAATTGGTAAAATGGAGAATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTa                                                                                                                                 <  3:204152/141‑1 (MQ=255)

             atataATCATTAAAATTGGTAAAATGGAGAATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTaa                                                                                                                                <  2:847106/141‑1 (MQ=255)

                taATCATTAAAATTGGTAAAATGGAGAATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATgc                                                                                                                             <  2:166721/141‑1 (MQ=255)

                 aaTCATTAAAATTGGTAAAATGGAGAATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATgcg                                                                                                                            <  2:997833/141‑1 (MQ=255)

                   tCATTAAAATTGGTAAAATGGAGAATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCt                                                                                                                          <  4:448630/141‑1 (MQ=255)

                   tCATTAAAATTGGTAAAATGGAGAATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCt                                                                                                                          >  1:173984/1‑141 (MQ=255)

                   tCATTAAAATTGGTAAAATGGAGAATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCt                                                                                                                          >  4:155075/1‑141 (MQ=255)

                   tCATTAAAATTGGTAAAATGGAGAATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCt                                                                                                                          >  2:756305/1‑141 (MQ=255)

                    cATTAAAATTGGTAAAATGGAGAATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCtt                                                                                                                         >  2:590700/1‑141 (MQ=255)

                       tAAAATTGGTAAAATGGAGAATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCaa                                                                                                                      <  1:142280/141‑1 (MQ=255)

                               gTAAAATGGAGAATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCCTTTATAAAATTAATGCGCTTCAAAAAGTAAc                                                                                                              >  2:1004780/1‑141 (MQ=255)

                                tAAAATGGAGAATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAAcc                                                                                                             <  3:75636/141‑1 (MQ=255)

                                  aaaTGGAGAATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGGGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCaa                                                                                                           <  3:308926/141‑1 (MQ=255)

                                    aTGGAGAATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAgc                                                                                                         <  1:306934/141‑1 (MQ=255)

                                     tGGAGAATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAtcg                                                                                                        <  2:153476/141‑1 (MQ=255)

                                     tGGAGAATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAgcg                                                                                                        <  1:912665/141‑1 (MQ=255)

                                      ggAGAATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAgcgc                                                                                                       <  1:355656/141‑1 (MQ=255)

                                      ggAGAATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTATTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAgcgc                                                                                                       >  1:852675/1‑141 (MQ=255)

                                       gagaATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTACTGCGCTTCAAAAAGTAACCAAGCGCa                                                                                                      <  1:148554/141‑1 (MQ=255)

                                        agaATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCaa                                                                                                     <  1:250112/141‑1 (MQ=255)

                                         gaATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAAt                                                                                                    >  3:399163/1‑141 (MQ=255)

                                         gaATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAAt                                                                                                    <  4:403693/141‑1 (MQ=255)

                                         gaATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAAt                                                                                                    <  2:134736/141‑1 (MQ=255)

                                         gaATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAAt                                                                                                    >  2:6024/1‑141 (MQ=255)

                                          aaTGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAAtt                                                                                                   <  3:286423/141‑1 (MQ=255)

                                           aTGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGGGTGTGACATTAAGGACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATta                                                                                                  <  1:213154/141‑1 (MQ=255)

                                              aCAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATtaata                                                                                               >  2:662968/1‑141 (MQ=255)

                                                 aTTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCCTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTaataaaa                                                                                            <  2:557521/141‑1 (MQ=255)

                                                   tttAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTaataaaata                                                                                          >  2:32988/1‑141 (MQ=255)

                                                     tAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAaataat                                                                                        <  4:447145/141‑1 (MQ=255)

                                                          cTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTGACCAAGCGCAATTAATAAAATAATCATAg                                                                                   <  2:147475/141‑1 (MQ=255)

                                                            gCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTa                                                                                 <  1:733306/141‑1 (MQ=255)

                                                            gCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCCTTTATAAAATTAATGCGCTTCAAAAAGTAATCAAGCGCAATTAATAAAATAGTCATAGTa                                                                                 <  4:412697/141‑1 (MQ=255)

                                                            gCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCCTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTa                                                                                 <  2:233590/141‑1 (MQ=255)

                                                              aCTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTaaa                                                                               <  3:344264/141‑1 (MQ=255)

                                                                  tttAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCCTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGt                                                                           <  2:3641/141‑1 (MQ=255)

                                                                  tttAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCCTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGt                                                                           <  1:304176/141‑1 (MQ=255)

                                                                    tAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGGCTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAACAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGttt                                                                         >  1:904335/1‑140 (MQ=255)

                                                                    tAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTc                                                                         >  2:249024/1‑141 (MQ=255)

                                                                      aaTTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTg                                                                       >  3:319059/1‑141 (MQ=255)

                                                                       actCACAATAACTAAAGATGGGAGTGTGACATTAAAAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAATAAATTGTTCTGt                                                                      >  2:159810/3‑141 (MQ=255)

                                                                           acaATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACt                                                                  >  1:745454/1‑141 (MQ=255)

                                                                           acaATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACt                                                                  >  2:616941/1‑141 (MQ=255)

                                                                            caATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAAAGCACTACAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTg                                                                 <  2:611538/141‑1 (MQ=255)

                                                                              aTAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGtt                                                               >  1:246845/1‑141 (MQ=255)

                                                                              aTAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGtt                                                               >  4:454983/1‑141 (MQ=255)

                                                                                 aCTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGTTATc                                                            >  1:367019/1‑141 (MQ=255)

                                                                                 aCTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCAGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGTTATc                                                            >  1:550792/1‑141 (MQ=255)

                                                                                   tAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGTTATccc                                                          <  2:493404/141‑1 (MQ=255)

                                                                                       gATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGTTATCCcttct                                                      <  3:311343/141‑1 (MQ=255)

                                                                                        aTGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGTTATCCcttctt                                                     <  2:379466/141‑1 (MQ=255)

                                                                                        aTGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGTTATCCcttctt                                                     >  1:1068937/1‑141 (MQ=255)

                                                                                           ggAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGTTATCCCTTCTTTCa                                                  >  3:349468/1‑141 (MQ=255)

                                                                                            gAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGTTATCCCTTCTTTCaa                                                 >  2:832189/1‑141 (MQ=255)

                                                                                            gAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGTTATCCCTTCTTTCaa                                                 >  1:717306/1‑141 (MQ=255)

                                                                                              gtgtGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGTTATCCCTTCTTTCaata                                               <  4:191957/141‑1 (MQ=255)

                                                                                                  gACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGTTATCCCTTCTTTCAGTAATCt                                           >  2:1036981/1‑141 (MQ=255)

                                                                                                     aTTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGTTATACCTTCTTTCAATAATCTTCa                                        >  4:456547/1‑141 (MQ=255)

                                                                                                      ttAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGTTATCCCTTCTTTCAATAATCTTCAt                                       >  2:940251/1‑141 (MQ=255)

                                                                                                      ttAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGTTATCCCTTCTTTCAATAATCTTCAt                                       <  3:276786/141‑1 (MQ=255)

                                                                                                      ttAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGTTATCACTTCTTTCAATAATCTTCAt                                       >  4:339813/1‑141 (MQ=255)

                                                                                                          gAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCa                                                                                      <  2:758/90‑1 (MQ=255)

                                                                                                          gAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCa                                                                                      >  1:758/1‑90 (MQ=255)

                                                                                                          gAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGTTATCCCTTCTTTCAATAATCTTCATAAtt                                   <  2:490826/141‑1 (MQ=255)

                                                                                                             cTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGTTATCCCTTCTTTCAATAATCTTCATAATTtat                                <  1:627501/141‑1 (MQ=255)

                                                                                                             cTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGTTATCCCTTCTTTCAATAATCTTCATAATTtat                                <  1:966451/141‑1 (MQ=255)

                                                                                                              tAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCCCTTCAAAAAGTATCCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGTTATCCCTTCTTTCAATAATCTTCATAATTtata                               <  2:23645/141‑1 (MQ=255)

                                                                                                               aaTGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGTTATCCCTTCTTTCAATAATCTTCATAATTTATaa                              >  1:725739/1‑141 (MQ=255)

                                                                                                                 tGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGTTATCCCTTCTTTCAATAATCTTCATAATTTATAACt                            <  2:89500/141‑1 (MQ=255)

                                                                                                                   tAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGTTATCCCTTCTTTCAATAATCTTCATAATTTATAACttt                          <  4:222281/141‑1 (MQ=255)

                                                                                                                    aGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGTTATCCCTTCTTTCAATAATCTTCATAATTTATAACTTTa                         <  3:167411/141‑1 (MQ=255)

                                                                                                                    aGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGTTATCCCTTCTTTCAATAATCTTCATAATTTATAACTTTa                         <  4:272514/141‑1 (MQ=255)

                                                                                                                     gCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGTTATCCCTTCTTTCAATAATCTTCATAATTTATAACTTTaa                        >  2:8019/1‑141 (MQ=255)

                                                                                                                       aaTGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGTTATCCCTTCTTTCAATAATCTTCATAATTTATAACTTTAACa                      <  1:654329/141‑1 (MQ=255)

                                                                                                                        aTGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGTTATCCCTTCTTTCAATAATCTTCATAATTTATAACTTTAACAt                     >  2:78186/1‑141 (MQ=255)

                                                                                                                             ttgactATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGGAAAATGTTCTGTCACTGTTATCCCTTCTTTCAATAATCTTCATAATTTATAACATAAACATACTcc                >  4:361630/1‑141 (MQ=255)

                                                                                                                              tgactATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGTTATCCCTTCTTTCAATAATCTTCATAATTTATAACTTTAACATACTCca               <  4:115946/141‑1 (MQ=255)

                                                                                                                              tgactATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGTTATCCCTTCTTTCAATAATCTTCATAATTTATAACTTTAACATACTCca               <  1:39033/141‑1 (MQ=255)

                                                                                                                              tgactATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGTTATCCCTTCTTTCAATAATCTTCATAATTTATAACTTTAACATACTCca               <  1:1015373/141‑1 (MQ=255)

                                                                                                                                actATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGTTATCCCTTCTTTCAATAATCTTCATAATTTATAACTTTAACATTCTCcaca             <  3:401924/141‑1 (MQ=255)

                                                                                                                                 ctATCTGTTGCTTTTATAAAATTAATGCGCTTCAAACAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGTTATCCCTTCTTTCAATAATCTTCATAATTTATAACTTTAACATACTCCACAg            <  2:244890/141‑1 (MQ=255)

                                                                                                                                   aTCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTTTCACTGTTATCCCTTCTTTCAATAATCTTCATAATTTATAACTTTAACATACTCCACAGat          <  2:432404/141‑1 (MQ=255)

                                                                                                                                   aTCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGTTATCCCTTCTTTCAATAATCTTCATAATTTATAACTTTAACATACTCCACAGat          <  3:284879/141‑1 (MQ=255)

                                                                                                                                   aTCTATTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCGTAGTAAATTGTTCTGTCACTGTTATCCCTTCTTTCAATAATCTTCATAATTTATAACTTTAACATTCTCCGCAGat          <  2:695942/141‑1 (MQ=255)

                                                                                                                                     cTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGTTATCCCTTCTTTCAATAATCTTCATAATTTATAACTTTAACATACTCCACAGatat        <  2:941892/141‑1 (MQ=255)

                                                                                                                                      tGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCCATGTTATCCCTTCTTTCAATAATCTTCATAATTTATAACTTTAACATACTCTACAGATAtt       >  4:310908/1‑141 (MQ=255)

                                                                                                                                      tGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGTTAt                                                             <  4:208440/87‑1 (MQ=255)

                                                                                                                                        ttGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGTTATCCCTTCTTTCAATAATCTTCATAATTTATAACTTTAACATACTCCACAGATAtttt     >  1:999312/1‑141 (MQ=255)

                                                                                                                                         tGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGTTATCCCTTCTTTCAATAATCTTCATAATTTATAACTTTAACATACTCCACAGATATTTTa    <  1:616941/141‑1 (MQ=255)

                                                                                                                                           cTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGTTATCCCTTCTTTCAATAATCTTCATAATTTATAACTTTAACATACTCCACAGATATTTTAGa  >  1:402488/1‑141 (MQ=255)

GCCACAATACCATATATAATCATTAAAATTGGTAAAATGGAGAATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGTTATCCCTTCTTTCAATAATCTTCATAATTTATAACTTTAACATACTCCACAGATATTTTAGA  >  USA300TCH1516_ALE/2379254‑2379533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`