Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 2,427,156 T→G 5.2% R342R (CGA→CGC USA300HOU_RS12500 ← Putative formate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,427,1560TG 5.2% 414.3 / 11.0 138R342R (CGA→CGCUSA300HOU_RS12500Putative formate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (63/67);  new base G (4/4);  total (67/71)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 143 total aligned reads displayed.

GAAGAAGAAATGAATGATAGTGCATCTGGGCCATGCTTTTCTTTAATAGCTGTAAAATTATCTGCAATGACGTTTAAAGCTTCATCCCATTCTACTTCATGGAACTCACCATTTTTCCTTACTAGTGGTTTAGTTAATCGTTGATCTGAATTAATATGTCCCCATGAAAACTTACCTTTAACACAAGTCGCAATTTTATTTGCTGGAGAATCATGTGATGGTTGTACTTTTAAAATTTCTCTATCTTTAGTCCAAACTTCAAATGAACAACCCACACC  >  USA300TCH1516_ALE/2427019‑2427296
                                                                                                                                         |                                                                                                                                            
gaagaagaaATGAATGATAGTGCATCTGGGCCATGCTTTTCTTTAATAGCTGTAAAATTATCTGCAATGACGTTTAAAGCTTCATCCCATTCTACTTCATGGAACTCACCATTTTTCCTTACTAGTGGTTTAGTTAATCGt                                                                                                                                           <  1:670177/141‑1 (MQ=255)
gaagaagaaATGAATGATAGTGCATCTGGGCCATGCTTTTCTTTAATAGCTGTAAAATTATCTGCAATGACGTTTAAAGCTTCATCCCATTCTACTTCATGGAACTCACCATTTTTCCTTACTAGTGGTTTAGTTAATCGt                                                                                                                                           <  1:966550/141‑1 (MQ=255)
 aagaagaaATGAATGATAGTGCATCTGGGCCATGCTTTTCTTTAATAGCTGTAAAATTATCTGCAATGACGTTTAAAGCTTCATCCCATTCTACTTCATGGAACTCACCATTTTTCCTTACTAGTGGTTTAGTTAATCGtt                                                                                                                                          >  1:865021/1‑141 (MQ=255)
    aagaaATGAATGATAGTGCATCTGGGCCATGCTTTTCTTTAATAGCTGTAAAATTATCTGCAATGACGTTTAAAGCTTCATCCCATTCTACTTCATGGAACTCACCATTTTTCCTTACTAGTGGTTTAGTTAATCGTTGAt                                                                                                                                       <  1:498531/141‑1 (MQ=255)
    aagaaATGAATGATAGTGCATCTGGGCCATGCTTTTCTTTAATAGCTGTAAAATTATCTGCAATGACGTTTAAAGCTTCATCCCATTCTACTTCATGGAACTCACCATTTTTCCTTACTAGTGGTTTAGTTAATCGTTGAt                                                                                                                                       <  1:447233/141‑1 (MQ=255)
     agaaATGAATGATAGTGCATCTGGGCCATGCTTTTCTTTAATAGCTGTAAAATTATCTGCAATGACGTTTAAAGCTTCATCCCATTCTACTTCATGGAACTCACCATTTTTCCTTACTAGTGGTTTAGTTAATCGTTGATc                                                                                                                                      >  1:684832/1‑141 (MQ=255)
     agaaATGAATGATAGTGCATCTGGGCCATGCTTTTCTTTAATAGCTGTAAAATTATCTGCAATGACGTTTAAAGCTTCATCCCATTCTACTTCATGGAACTCACCATTTTTCCTTACTAGTGGTTTAGTTAATCGTTGATc                                                                                                                                      <  3:35817/141‑1 (MQ=255)
     agaaATGAATGATAGTGCATCTGGGCCATGCTTTTCTTTAATAGCTGTAAAATTATCTGCAATGACGTTTAAAGCTTCATCCCATTCTACTTCATGGAACTCACCATTTTTCCTTACTAGTGGTTTAGTTAATCGTTGATc                                                                                                                                      <  3:44179/141‑1 (MQ=255)
     agaaATGAATGATAGTGCATCTGGGCCATGCTTTTCTTTAATAGCTGTAAAAGTATCTGCAATGACGTTTAAAGCTTCATCCCATTCTACTTCATGGAACTCACCATTTTTCCTTACTAGTGGTTTAGTTAATCGTTGATc                                                                                                                                      <  2:181067/141‑1 (MQ=255)
       aaaTTAATGATATTGCATCTGGGCCTTACTTTTCTTTAGTATATGTAAAGTTATCTGCAATGACATTTAAATCTTCATCCCATTCTACTTCATGGAACTCACCATTTTTCCTTACTAGTGGTTTAGTTAAGCGTTGATCTg                                                                                                                                    <  3:19871/141‑1 (MQ=255)
        aatgaatgATAGTGCATCTGGGCCATGCTTTTCTTTAATAGCTGTAAAATTATCTGCAATGACGTTTAAAGCTTCATCCCATTCTACTTCATGGAACTCACCATTTTTCCTTACTAGTGGTTTAGTTAATCGTTGATCTGa                                                                                                                                   >  2:1046638/1‑141 (MQ=255)
        aatgaatgATAGTGCATCTGGGCCATGCTTTTCTTTAATAGCTGTAAAATTATCTGCAATGACGTTTAAAGCTTCATCCCATTCTACTTCATGGAACTCACCATTTTTCCTTACTAGTGGTTTAGTTAATCGTTGATCTGa                                                                                                                                   >  2:849456/1‑141 (MQ=255)
          tgaatgaTAGTGCATCTGGGCCCTGCTTTTCTTTAACAGCTGTAAAATTATCCGCAATGACGTTTAAAGCTTCATCCCACTCTACTTCATGGAACTCACCATTTTTCCTTACTAGTGGCTCAGTTAATCGTTGACCTGAAt                                                                                                                                 >  2:192983/1‑141 (MQ=255)
          tgaatgaTAGTGCATCTGGGCCATGCTTTTCTTTAATAGCTGTAAAATTATCTGCAATGACCTTTAAAGCTTCATCCCATTCTACTTCATGGAACTCACCATTTTTCCTTACTAGTGGTTTAGTTAATCGTTGCTCTGatt                                                                                                                                 >  2:22496/1‑139 (MQ=255)
             atgaTAGTGCATCTGGGCCATGCGTTTCTTTAATAGCTGTAAAATTATCTGCAATGCCGTTTAAAGCTTCATCCCATTCTACTTCATGGAACTCACCATTTTTCCTTACTAGTGGTTTAGTTAATCGTTGATCTGAATTaa                                                                                                                              >  2:514111/1‑141 (MQ=255)
                aTAGTGCATCTGGGCCATACTTTTCTTTAATAGATGTAAAATTATCTGCAATGACATTTAAAGCTTCATCCCATTCTACTTCATGGAACTCACCATTTTTCCTTACTAGTGGTTTAGTTAAGCGTTGATCTGAATTAatat                                                                                                                           <  2:387331/141‑1 (MQ=255)
                 tAGTGCATCTGGGCCATGCTTTTCTTTAATAGCTGTAAAATTATCTGCAATGACGTTTAAAGCTTCATCCCATTCTACTTCATGGAACTCACCATTTTTCCTTACTAGTGGTTTAGTTAATCGTTGATCTGAATTAATATg                                                                                                                          <  1:677307/141‑1 (MQ=255)
                  aGTGCATCTGGGCCATGCTTTTCTTTAATAGCTGTAAAATTATCTGCAATGACGTTTAAAGCTTCATCCCATTCTACTTCATGGAACTCACCATTTTTCCTTACTAGTGGTTTAGTTAATCGTTGATCTGAATTAATATGt                                                                                                                         >  2:694897/1‑141 (MQ=255)
                        tCTGGGCCATGCTTTTCTTTAATAGCTGTAAAATTATCTGCAATGACGTTTAAAGCTTCATCCCATTCTACTTCATGGAACTCACCATTTTTCCTTACTAGTGGTTTAGTTAATCGTTGATCTGAATTAATATGTCCCCAt                                                                                                                   <  1:595206/141‑1 (MQ=255)
                         cTGGGCCATGCTTTTCTTTAATAGCTGTAAAATTATCTGCAATGACGTTTAAAGCTTCATCCCATTCTACTTCATGGAACTCACCATTTTTCCTTACTAGTGGTTTAGTTAATCGTTGATCTGAATTAATATGTCCCCATg                                                                                                                  <  1:625903/141‑1 (MQ=255)
                                 tGCTTTTCTTTAATAGCTGTAAAATTATCTGCAATGACGTTTAAAGCTTCATCCCATTCTACTTCATGGAACTCACCATTTTTCCTTACTAGTGGTTTAGTTAATCGTTGATCTGAATTAATATGTCCCCATGAAAACTTa                                                                                                          <  1:56114/141‑1 (MQ=255)
                                  gCTTTTCTTTAATAGCTGTAAAATTATCTGCAATGACGTTTAAAGCTTCATCCCATTCTACTTCATGGAACTCACCATTTTTCCTTACTAGTGGTTTAGTTAATCGTTGATCCGAATTAATATGTCCCCATGAAAACTTAc                                                                                                         <  4:178958/141‑1 (MQ=255)
                                   cTTTTCTTTAATAGCTGTAAAATTATCTGCAATGACGTTTAAAGCTTCATCCCATTCTACTTCATGGAACTCACCATTTTTCCTTTTTTGTTGTTTAGTTAATCGTTGATCTGAATTAATATGTCCCCATGAAAACTTAcc                                                                                                        >  2:459742/1‑141 (MQ=255)
                                    ttttCTTTAATAGCTGTAAAATTATCTGCAATGACGTTTAAAGCTTCATCCCATTCTACTTCATGGAACTCACCATTTTTCCTTACTAGTGGTTTAGTTAATCGTTGATCTGAATTAATATGTCCCCATGAAAACTTACCt                                                                                                       <  4:192065/141‑1 (MQ=255)
                                    ttttCTTTAATAGCTGTAAAATTATCTGCAATGACGTTTAAAGCTTCATCCCATTCTACTTCATGGAACTCACCATTTTTCCTTACTAGTGGTTTAGTTAATCGTTGATCTGAATTAATATGTCCCCATGAAAACTTACCt                                                                                                       >  1:972287/1‑141 (MQ=255)
                                    ttttCTTTAATAGCTGTAAAATTATCTGCAATGACGTTTAAAGCTTCATCCCATTCTACTTCATGGAACTCACCAGTTGTCCTTACTAGTGGTTTAGTTAATCGTTGATCTGAATTAATATGTCCCCATGAAAACTTACCt                                                                                                       <  2:440253/141‑1 (MQ=255)
                                      ttCTTTAATAGCTGGAAAATTATCTGCTATGACGTTTAAAGCTTCATCCCATTCTTCTGCATGGAACTCACCATTTGGCCTTTCTATTGTGTTAGTTAATCGTTGATCTGAATTAGTATATCCCCATTAAAACTTACCttt                                                                                                     <  4:318936/141‑1 (MQ=255)
                                        cTTTAATAGCTGTAAAATTATCTGCAATGACGTTTAAAGCTTCATCCCATTCTACTTCATGGAACTCACCATTTTTCCTTACTAGTGGTTTAGTTAATCGTTGATCTGAATTAATATGTCCCCATGAAAACTTACCTTTaa                                                                                                   >  3:201475/1‑141 (MQ=255)
                                              tAGCTGTAAAATTATCTGCAATGACGTTTAAAGCTTCATCCCATTCTACTTCATGGAACTCACCATTTTTCCTTACTAGTGGTTTAGTTAATCGTTGATCTGAATTAAAATGTCCCCATGAAAACTTACCTTTAACACAAg                                                                                             <  3:141124/141‑1 (MQ=255)
                                                 cTGTAAAATTATCTGCAATGACGTTTAAAGCTTCATCCCATTCTACTTCATGGAACTCACCATTTTTCCTTACTAGTGGTTTAGTTAATCGTTGATCTGAATTAATATGTCCCCATGAAAACTTACCTTTAACACAAGTCg                                                                                          >  1:784959/1‑141 (MQ=255)
                                                  tGTAAAATTATCTGCAATGACGTTTAAAGCTTCATCCCATTCTACTTCATGGAACTCACCATTTTTCCTTACTAGTGGTTTAGTTAATCGTTGATCTGAATTAATATGTCCCCATGAAAACTTACCTTTAACACAAGTCGc                                                                                         >  1:38967/1‑141 (MQ=255)
                                                  tGTAAAATTATCTGCAATGACGTTTAAAGCTTCATCCCATTCTACTTCATGGAACTCACCATTTTTCCTTACTAGTGGTTTAGTTAATCGTTGATCTGAATTAATATGTCCCCATGAAAACTTACCTTTAACACAAGTCGc                                                                                         <  2:874595/141‑1 (MQ=255)
                                                        aTTATCTGCAATGACGTTTAAAGCTTCATCCCATTCTACTTCATGGAACTCACCATTTTTCCTTACTAGTGGTTTAGTTAATCGTTGATCTGAATTAATATGTCCCCATGAAAACTTACCTTTAACACAAGTCGCAAtttt                                                                                   <  3:334873/141‑1 (MQ=255)
                                                         ttATCTGCAATGACGTTTAAAGCTTCATCCCATTCTACTTCATGGAACTCACCATTTTTCCTTACTAGTGGTTTAGTTAATCGTTGATCTGAATTAATATGTCCCCATGAAAACTTACCTTTAACACAAGTCGCAATTTTa                                                                                  >  2:619923/1‑141 (MQ=255)
                                                         ttATCTGCAATGACGTTTAAAGCTTCATCCCATTCTACTTCATGGAACTCACCATTTTTCCTTACTAGTGGTTTAGTTAATCGTTGATCTGAATTAATATGTCCCCATGAAAACTTACCTTTAACACAAGTCGCAATTTTa                                                                                  <  2:1064080/141‑1 (MQ=255)
                                                           aTCTGCAATGACGTTTAAAGCTTCATCCCATTCTACTTCATGGAACTCACCATTTTTCCTTACTAGTGGTTTAGTTAATCGTTGATCTGAATTAATATGTCCCCATGAAAACTTACCTTTAACACAAGTCGCAATTTTAtt                                                                                <  2:922300/141‑1 (MQ=255)
                                                           aTCTGCAATGACGTTTAAAGCTTCATCCCATTCTACTTCATGGAACTCACCATTTTTCCTTACTAGTGGTTTAGTTAATCGTTGATCTGAATTAATATGTCCCCATGAAAACTTACCTTTAACACAAGTCGCAATTTTAtt                                                                                >  1:577407/1‑141 (MQ=255)
                                                           aTCTGCAATGACGTTTAAAGCTTCATCCCATTCTACTTCATGGAACTCACCATTTTTCCTTACTAGTGGTTTAGTTAATCGTTGATCTGAATTAATATGTCCCCATGAAAACTTACCTTTAACACAAGTCGCAATTTTAtt                                                                                >  1:1057365/1‑141 (MQ=255)
                                                           aTCTGCAATGACGTTTAAAGCTTCATCCCATTCTACTTAATGGAACTCACCATTTTTCCTCACTAAGGGGTTAACCACTGGTGGCTGGGAATTAATAAGTGCCCATGAAAACCTACCTTTAACACAAGTCGCAATTTTAtt                                                                                >  3:154532/1‑141 (MQ=255)
                                                            tCTGCAATGACGTTTAAAGCTTCATCCCATTCTACTTCATGGAACTCACCATTTTTCCTTACTAGTGGTTTAGTTAATCGTTGATCTGAATTAATATGTCCCCATGAAAACTTACCTTTAACACAAGTCGCAATTTTAttt                                                                               >  1:930148/1‑141 (MQ=255)
                                                                cAATGACGTTTAAAGCTTCATCCCATTCTACTTCATGGAACTCACCATTTTTCCTTACTAGTGGTTTAGTTAATCGTTGATCTGAATTAATATGTCCCCATGAAAACTTACCTTTAACACAAGTCGCAATTTTATTTGCTg                                                                           >  2:1061470/1‑141 (MQ=255)
                                                                 aaTGACGTTTAAAGCTTCATCCCATTCTACTTCATGGAACTCACCATTTTTCCTTACTAGTGGTTTAGTTAATCGTTGATCTGAATTAATATGTCCCCATGAAAACTTACCTTTGACACAAGTCGCAATTTTATTTGCTgg                                                                          <  2:710094/141‑1 (MQ=255)
                                                                  aTGACGTTTAAAGCTTCATCCCATTCTACTTCATGGAACTCACCATTTTTTTTTTTTTTTTTTTTAGTTAATCGCTGATCTGAATTAATATGTCCCCATGAAGACTTACCTTTAACCCAAGTCGCAATTTTATTTGCTGga                                                                         >  2:144233/1‑141 (MQ=255)
                                                                     aCGTTTAAAGCTTCATCCCATTCTACTTCATGGAACTCACCATTTTTCCTTACTAGTGGTTTAGTTAATCGTTGATCTGAATTAATATGTCCCCATGAAAACTTACCTTTAACACAAGTCGCAATTTTATTTGCTGGAGaa                                                                      <  2:489334/141‑1 (MQ=255)
                                                                      cGTTTAAAGCTTCATCCCATTCTACTTCATGGAACTCACCATTTTTCCTTACTAGTGGTTTAGTTAATCGTTGATCTGAATTAATATGTCCCCATGAAAACTTACCTTTAACACAAGTCGCAATTTTATTTGCTGGAGAAt                                                                     <  2:571333/141‑1 (MQ=255)
                                                                       gTTTAAAGCTTCATCCCATTCTACTTCATGGAACTCACCATTTTTTTTTTCTTTTGTTTTAGTTAGTCGTTGATCTGAATTAATATGTCCCCATGAAAACTTACCTTTAACACAAGTCGCACTTTTATTTGCTGGAGAATc                                                                    >  2:743177/1‑141 (MQ=255)
                                                                         ttAAAGCTTCATCCCATTCTACTTCATGGAACTCACCATTTTTCCTTACTAGTGGTTTAGTTAATCGTTGATCTGAATTAATATGTCCCCATGAAAACTTACCTTTAACACAAGTCGCAATTTTATTTGCTGGAGAATCAt                                                                  <  2:879805/141‑1 (MQ=255)
                                                                          tAAAGCTTCATCCCATTCTACTTCATGGAACTCACCATTTTTCCTTACTAGTGGTTTAGTTAATCGTTGATCTGAATTAATATGTCCCCATGAAAACTTACCTTTAACACAAGTCGCAATTTTATTTGCTGGAGAATCAtg                                                                 >  1:899765/1‑141 (MQ=255)
                                                                          tAAAGCTTCATCCCATTCTACTTCATGGAACTCACCATTTTTCCTTACTAGTGGTTTAGTTAATCGTTGATCTGAATTAATATGTCCCCATGAAAACTTACCTTTAACACAAGTCGAAATTTTATTTGCTGGAGAATCAtt                                                                 >  3:227443/1‑140 (MQ=255)
                                                                           aaaGCTTCATCCCATTCTACTTCATGGAACTCACCATTTTTCCTTACTAGTGGTTTAGTTAATCGTTGATCTGAATTAATATGTCCCCATGAAAACTTACCTTTAACACAAGTCGCAATTTTATTTGCTGGAGAATCAtgt                                                                >  3:50275/1‑141 (MQ=255)
                                                                           aaaGCTTCATCCCATTCTACTTCATGGAACTCACCATTTTTCCTTACTAGTGGTTTAGTTAATCGTTGATCTGAATTAATATGTCCCCATGAAAACTTACCTTTAACACAAGTCGCAATTTTATTTGCTGGAGAATCAtgt                                                                >  1:212692/1‑141 (MQ=255)
                                                                             aGCTTCATCCCATTCTACTTCATGGAACTCACCATTTTTCCTTACTAGTGGTTTAGTTAATCGTTGATCTGAATTAATATGTCCGCATGAAAACTTACCTTTAACACAAGTCGCAATTTTATTTGCTGGAGAATCATGGGa                                                              <  2:1038179/141‑1 (MQ=255)
                                                                               ttttagcccATTCTACGTGCTGGAACTCAGCGTTTTCCCTTACTTGAGGTTTAGTTAATCGTTGTTCTGAATTAATATGTCCCCTTGAAAACTTACCTTTGGCACCGGTCGCAATTTTATTTGCTGAAGAATCATGTGATg                                                            <  4:393288/135‑1 (MQ=255)
                                                                                 tCATCCCATTCTACTTCATGGAACTCACCATTTTTCCTTACTAGTGGTTTAGTTAAGCGTTGATCTGAATTAATATGTCCCCATGAAAACTTACCTTTAACACAAGTCGCAATTTTATTTGCTGGAGAATCATGTGATGGt                                                          >  1:848879/1‑141 (MQ=255)
                                                                                  cATCCCATTCTACTTCATGGAACTCACCATTTTTTTTTTTTTTTTTTTTAGTTAATCGTTGATCTGAATTAATATGTCCCCATGAAAACTTACCTTTAACACAAGTCGCAATTTTATTTGCTGGAGAATCATGTGATGGtt                                                         >  2:409754/1‑141 (MQ=255)
                                                                                  cATCCCATTCTACTTCATGGAACTCACCATTTTTCCTTACTAGTGGTTTAGTTAATCGTTGATCTGAATTAATATGTCCCCATGAAAACTTACCTTTAACACAAGTCGCAATTTTATTTGCTGGAGAATCATGTGATGGtt                                                         >  2:409815/1‑141 (MQ=255)
                                                                                   aTCCCATTCTACTTCATGGAACTCACCATTTTTCCTTACTAGTGGTTTAGTTAATCGTTGATCTGAATTAATATGTCCCCATGAAAACTTACCTTTAACACAAGTCGCAATTTTATTTGCTGGAGAATCATGTGATGGTTg                                                        <  2:752618/141‑1 (MQ=255)
                                                                                    tCCCATTCTACTTCATGGAACTCACCATTTTTCCTTACTAGTGGTTTAGTTAATCGTTGATCTGAATTAATATGTCCCCATGAAAACTTACCTTTAACACAAGTCGCAATTTTATTTGCTGGAGAATCATGTGATGGTTGt                                                       >  2:194757/1‑141 (MQ=255)
                                                                                         ttCTACTTCATGGAACTCACCATTTTTCCTTACTAGTGGTTTAGTTAATCGTTGATCTGAATTAATATGTCCCCATGAAAACTTACCTTTAACACAAGTCGCAATTTTATTTGCTGGAGAATCATGTGATGGTTGTACttt                                                  <  3:79829/141‑1 (MQ=255)
                                                                                         ttCTACTTCATGGAACTCACCATTTTTCCTTACTAGTGGTTTAGTTAATCGTTGATCTGAATTAATATGTCCCCATGAAAACTTACCTTTAACACAAGTCGCAATTTTATTTGCTGGAGAATCATGTGATGGTTGTACttt                                                  >  3:404746/1‑141 (MQ=255)
                                                                                          tCTACTTCATGGAACTCACCATTTTTCCTTACTAGTGGTTTAGTTAATCGTTGATCTGAATTAATATGTCCCCATGAAAACTTACCTTTAACACAAGTCGCAATTTTATTTGCTGGAGAATCATGTGATGGTTGTACtttt                                                 <  1:597348/141‑1 (MQ=255)
                                                                                              cTTCATGGAACTCACCATTTTTCCTTACTAGTGGTTTAGTTAATCGTTGATCTGAATTAATATGTCCCCATGAAAACTTACCTTTAACACAAGTCGCAATTTTATTTGCTGGAGAATCATGTGATGGTTGTACTTTTaaaa                                             >  1:35339/1‑141 (MQ=255)
                                                                                               ttCATGGAACTCACCATTTTTCCTTACTAGTGGTTTAGTTAATCGTTGATCTGAATTAATATGTCCCCATGAAAACTTACCTTTAACACAAGTCGCAATTTTATTTGCTGGAGAATCATGTGATGGTTGTACTTTTAAAAt                                            >  1:610204/1‑141 (MQ=255)
                                                                                                 cATGGAACTCACCATTTTTCCTTACTAGTGGTTTAGTTAATCGTTGATCTGAATTAATATGTCCCCATGAAAACTTACCTTTAACACAAGTCGCAATTTTATTTGCTGGAGAATCATGTGATGGTTGTACTTTTAAAAttt                                          <  2:5996/141‑1 (MQ=255)
                                                                                                 cATGGAACTCACCATTTTTCCTTACTAGTGGTTTAGTTAATCGTTGATCTGAATTAATATGTCCCCATGAAAACTTACCTTTAACACAAGTCGCAATTTTATTTGCTGGAGAATCATGTGATGGTTGTACTTTTAAAAttt                                          >  2:35359/1‑141 (MQ=255)
                                                                                                    ggAACTCACCATTTTTCCTTACTAGTGGTTTAGTTAATCGTTGATCTGACTTAATATGTCCCCATGAAAACTTACCTTTAACACAAGTCGCAATTTTATTTGCTGGAGAATCATGTGATGGTTGTGCTTTTAAAATTtctc                                       >  2:615942/1‑141 (MQ=255)
                                                                                                    ggAACTCACCATTTTTCCTTACTAGTGGTTTAGTTAATCGTTGATCTGAATTAATATGTCCCCATGAAAACTTACCTTTAACACAAGTCGCAATTTTATTTGCTGGAGAATCATGTGATGGTTGTACTTTTAAAATTtctc                                       <  4:290938/141‑1 (MQ=255)
                                                                                                      aaCCCACCATTTTTCCTTACTAGTGGTATAGTCCGGCGTTGACCTGAATTAATATGTCCCCATGAAAACTTACCTTTAACACAAGTCGCAATTTTATTTGCTGGAGAATCATGTGATGGTTGTACTTTAAAAATTTCTCTa                                     >  1:29854/1‑141 (MQ=255)
                                                                                                       aCTCACCATTTTTCCTTACTAGTGGTTTAGTTAATCGTTGATCTGAATTAATATGTCCCCATGAAAACTTACCTTTAACACAAGTCGCAATTTTATTTGCTGGAGAATCATGTGATGGTTGTACTTTTAAAATTTCTCTAt                                    >  1:739115/1‑141 (MQ=255)
                                                                                                        cTCACCATTTTTCCTTACTAGTGGTTTAGTTAATCGTTGATCTGAATTAATATGTCCCCATGAAAACTTACCTTTAACACAAGTCGCAATTTTATTTGCTGGAGAATCATGTGATGGTTGTACTTTTAAAATTTCTCTATc                                   >  3:402490/1‑141 (MQ=255)
                                                                                                        cTCACCATTTTTCCTTACTAGTGGTTTAGTTAATCGTTGATCTGAATTAATATGTCCCCATGAAAACTTACCTTTAACACAAGTCGCAATTTTATTTGCTGGAGAATCATGTGATGGTTGTACTTTTAAAATTTCTCTATc                                   >  3:402488/1‑141 (MQ=255)
                                                                                                        cTCACCATTTTTCCTTACTAGTGGTTTAGTTAATCGTTGATCTGAATTAATATGTCCCCATGAAAACTTACCTTTAACACAAGTCGCAATTTTATTTGCTGGAGAATCATGTGATGGTTGTACTTTTAAAATTTCTCTATc                                   >  3:288277/1‑141 (MQ=255)
                                                                                                         tCACCATTTTTCCTTACTAGTGGTTTAGTTAATCGTTGATCTGAATTAGTATGTCCCCATGAAAACTTACCTTTAACACAAGTCGCAATTTTATTAGCTGGAGAATCATGTGATGGTTGTACTTTTAAACTTTCTCTATCt                                  >  2:587599/1‑141 (MQ=255)
                                                                                                         tCACCATTTTTCCTTACTAGTGGTTTAGTTAATCGTTGATCTGAATTAATATGTCCCCATGAAAACTTACCTTTAACACAATTCGCACTTTTATTTGCTGGAGAATCATGTGATGGTTGTACTTTTAAAATTTGTCTATCt                                  >  1:113825/1‑141 (MQ=255)
                                                                                                          cACCATTTTTCCTTACTAGTGGTTTAGTTAATCGTTGATCTGAATTAATATGTCCCCATGAAAACTTACCTTTAACACAAGTCGCAATTTTATTTGCTGGAGAATCATGTGATGGTTGTACTTTTAAAATTTCTCTATCtt                                 <  2:946885/141‑1 (MQ=255)
                                                                                                           ccgaTTTTTCCTTGCTAGTTGTTTAGTTGATCGTTGATATCAAGTAATATGTGCGCATGAAATCTTAACATTAACACAAGTCGCTATTTTATTTGCTGGAGAATCATGTGATGGAGGTACTATTAAAACATCTCTATCttg                                <  4:397770/138‑2 (MQ=255)
                                                                                                              aTTTTTCCTTACTAGTGGTTTAGTTAATCGTTGATCTGAATTAATATGTCCCCATGAAAACTTACCTTTAACACAAGTCGCAATTTTATTTGCTGGAGAATCATGTGATGGTTGTACTTTTAAAATTTCTCTATCTTTAGt                             <  4:124110/141‑1 (MQ=255)
                                                                                                                ttttCCTTACTAGTGGTTTAGTTAATCGTTGATCTGAATTAATATGTCCCCATGAAAACTTACCTTTAACACAAGTCGCAATTTTATTTGCTGGAGAATCATGTGATGGTTGTACTTTTAAAATTTCTCTATCTTTAGTcc                           <  1:821847/141‑1 (MQ=255)
                                                                                                                ttttCCTTACTAGTGGTTTAGTTAATCGTTGATCTGAATTAATATGTCCCCATGAAAACTTACCTTTAACACAAGTCGCAATTTTATTTGCTGGAGAATCATGTGATGGTTGTACTTTTAAAATTTCTCTATCTTTAGTcc                           <  1:22989/141‑1 (MQ=255)
                                                                                                                 tttCCTTACTAGTGGTTTAGTTAATCGTTGATCTGAATTAATATGTCCCCATGAAAACTTACCTTTAACACAAGTCGCAATTTTATTTGCTGGAGAATCATGTGATGGTTGTACTTTTAAAATTTCTCTATCTTTAGTCCa                          <  1:16298/141‑1 (MQ=255)
                                                                                                                  ttCCTTACTAGTGGTTTAGTTAATCGTTGATCTGAATTAATATGTCCCCATGAAAACTTACCTTTAACACAAGTCGCAATTTTATTTGCTGGAGAATCATGTGATGGTTGTACTTTTAAAATTTCTCTATCTTTAGTCCaa                         <  4:346814/141‑1 (MQ=255)
                                                                                                                    ccTTACTAGTGGTTTAGTTAATCGTTGATCTGAATTAATATGTCCCCATGAAAACTTACCTTTAACACAAGTCGCAATTTTATTTGCTGGAGAATCATGTGATGGTTGTACTTTTAAAATTTCTCTATCTTTAGTCCAAAc                       >  3:121390/1‑141 (MQ=255)
                                                                                                                      ttACTAGTGGTTTAGTTAATCGTTGATCTGAATTAATATGTCCCCATGAAAACTTACCTTTAACACAAGTCGCAATTTTATTTGCTGGAGAATCATGTGATGGTTGTACTTTTAAAATTTCTCTATCTTTAGTCCAAACtt                     >  2:84824/1‑141 (MQ=255)
                                                                                                                           ggTGGTTTAGTTAATCGTTGATCTGAATTAATATGTCCCCATGAAAACTTACCTTTAACACAAGTCGCAATTTTATTTGCTGGAGAATCATGTGATGGTTGTACTTTTAAAATTTCTCTATCTTTAGTCCAAACTTCAAAt                <  2:974415/140‑1 (MQ=255)
                                                                                                                           aGTGGTTTAGTTAATCGTTGATCTGAATTAATATGTCCCCATGAAAACTTACCTTTAACACAAGTCGCAATTTTATTTGCTGGAGAATCATGTGATGGTTGTACTTTTAAAATTTCTCTATCTTTAGTCCAAACTTCAAAt                <  2:325101/141‑1 (MQ=255)
                                                                                                                            ttGGTTTAGTTAATCGTTGATCTGAATTAATATGTCCCCATGAAAACTTACCTTTAACACAAGTCGCAATTTTATTTGCTGGAGAATCATGTGATGGTTGTACTTTTAAAATTTCTCTATCTTTAGTCCAAACTTCAAATg               >  1:1029774/2‑141 (MQ=255)
                                                                                                                            gTGGTTTAGTTAAGCGTTGATCTGCATTAATATGTCCCCATGAAAAGTTACCATTAACATTGGTAGTACTTATATTCGTTTGAGAATCATGTGATGGTTGTACTTTTAAAATTTCTCTATCTTTAGTCCAAACTTCAAATg               <  1:918427/141‑1 (MQ=255)
                                                                                                                               gTTTAGTTAATCGTTGATCTGAATTAATATGTCCCCATGAAAACTTACCTTTAACACAAGTCGCAATTTTATTTGCTGGAGAATCATGTGATGGTTGTACTTTTAAAATTTCTCTATCTTTAGTCCAAACTTCAAATGaac            <  4:246779/141‑1 (MQ=255)
                                                                                                                                 ttAGTTAATCGTTGATCTGAATTAATATGTCCCCATGAAAACTTACCTTTAACACAAGTCGCAATTTTATTTGCTGGAGAATCATGTGATGGTTGTACTTTTAAAATTTCTCTATCTTTAGTCCAAACTTCAAATGaacaa          <  2:253497/141‑1 (MQ=255)
                                                                                                                                 ttAGTTAATCGTTGATCTGAATTAATATGTCCCCATGAAAACTTACCTTTAACACAAGTCGCAATTTTATTTGCTGGAGAATCATGTGATGGTTGTACGTTTAAAATTTCTCTATCTTTAGTCCAAACTTCAAACGaacaa          >  2:1039114/1‑141 (MQ=255)
                                                                                                                                  tAGTTAATCGTTGATCTGAATTAATATGTCCCCATGAAAACTTACCTTTAACACAAGTCGCAATTTTATTTGCTGGAGAATCATGTGATGGTTGTACTTTTAAAATTTCTCTATCTTTAGTCCAAACTTCAAATGaacaac         <  1:514133/141‑1 (MQ=255)
                                                                                                                                  tAGTTAATCGTTGATCTGAATTAATATGTCCCCATGAAAACTTACCTTTAACACAAGTCGCAATTTTATTTGCTGGAGAATCATGTGATGGTTGTACTTTTAAAATTTCTCTATCTTTAGTCCAAACTTCAAATGaacaac         <  2:309965/141‑1 (MQ=255)
                                                                                                                                   aGTTAATCGTTGATCTGAATTAATATGTCCCCATGAAAACTTACCTTTAACACAAGTCGCAATTTTATTTGCTGGAGAATCATGTGATGGTTGTACTTTTAAAATTTCTCTATCTTTAGTCCAAACTTCAAATGAACAAcc        <  3:440979/141‑1 (MQ=255)
                                                                                                                                   aGTTAATCGTTGATCTGAATTAATATGTCCCCATGAAAACTTACCTTTAACACAAGTCGCAATTTTATTTGCTGGAGAATCATGTGATGGTTGTACTTTTAAAATTTCTCTATCTTTAGTCCAAACTTCAAATGAACAAcc        >  3:293924/1‑141 (MQ=255)
                                                                                                                                     ttAATCGTTGATCTGAATTAATATGTCCCCATGAAAACTTACCTTTAACACAAGTCGCAATTTTATTTGCTGGAGAATCATGTGATGGTTGTACTTTTAAAATTTCTCTATCTTTAGTCCAAACTTCAAATGAACAACcca      >  1:862126/1‑141 (MQ=255)
                                                                                                                                     ttAAGCGTTGATCTGAATTAATATGTCCCCATGAAAACTTACCTTTAACACAAGTCGCAATTTTATTTGCTGGAGAATCATGTGATGGTTGTACTTTTAAAATTTCTCTATCTTTAGTCCAAACTTCAAATGAACAACcca      >  3:127550/1‑141 (MQ=255)
                                                                                                                                      tAATCGTTGATCTGAATTAATATGTCCCCATGACAACTTACCTTTAACACAAGTCGCAATTTTATTTGCTGGAGAATCATGTGATGGTTGTACTTTTAAAATTTCTCTATCTTTAGTCCAAACTTCAAATGAACAACccac     >  1:563380/1‑141 (MQ=255)
                                                                                                                                         tCGTTGATCTGAATTAATATGTCCCCATGAAAACTTACCTTTAACACAAGTCGCAATTTTATTTGCTGGAGAATCATGTGATGGTTGTACTTTTAAAATTTCTCTATCTTTAGTCCAAACTTCAAATGAACAACccacacc  <  2:832045/141‑1 (MQ=255)
                                                                                                                                         tCGTTGATCTGAATTAATATGTCCCCATGAAAACTTACCTTTAACACAAGTCGCAATTTTATTTGCTGGAGAATCATGTGATGGTTGTACTTTTAAAATTTCTCTATCTTTAGTCCAAACTTCAAATGAACAACccacacc  <  1:444737/141‑1 (MQ=255)
                                                                                                                                         tCGTTGATCTGAATTAATATGTCCCCATGAAAACTTACCTTTAACACAAGTCGCAATTTTATTTGCTGGAGAATCATGTGATGGTTGTACTTTTAAAATTTCTCTATCTTTAGTCCAAACTTCAAATGAACAACccacacc  <  1:162497/141‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                            
GAAGAAGAAATGAATGATAGTGCATCTGGGCCATGCTTTTCTTTAATAGCTGTAAAATTATCTGCAATGACGTTTAAAGCTTCATCCCATTCTACTTCATGGAACTCACCATTTTTCCTTACTAGTGGTTTAGTTAATCGTTGATCTGAATTAATATGTCCCCATGAAAACTTACCTTTAACACAAGTCGCAATTTTATTTGCTGGAGAATCATGTGATGGTTGTACTTTTAAAATTTCTCTATCTTTAGTCCAAACTTCAAATGAACAACCCACACC  >  USA300TCH1516_ALE/2427019‑2427296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: