Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 2,479,526 A→G 5.3% G108G (GGT→GGC hrtA_2 ← Putative hemin import ATP‑binding protein HrtA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,479,5260AG 5.3% 322.4 / 5.4 107G108G (GGT→GGChrtA_2Putative hemin import ATP‑binding protein HrtA
Reads supporting (aligned to +/- strand):  ref base A (35/66);  new base G (2/4);  total (37/70)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01

Only 100 of 111 total aligned reads displayed.

GCTCTCATAATCGCAACACGTTGCTTTTCACCGCCAGATAACTGATGCGGATATACATTCAAGCGATCTTCTAAACCAATATTTTTCAAAAGTTGTATTGCTCTTGTTGAACTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACTAAATGTGAAGATTGAAAAATAAAACCAATATCTTCCAATCGTAAATCACTAGGACGATGCTGTTTATCAAACAATGGCGCATCATTGTAAAGCACTGT  >  USA300TCH1516_ALE/2479386‑2479666
                                                                                                                                            |                                                                                                                                            
gCTCTCATAATCGCAACACGTTGCTTTTCACCGCCAGATAACTGATGCGGATATACATTCAAGCGATCTTCTAAACCAATATTTTTCAAAAGTTGTATTGCTCTTGTTGAACTTTGTTGTTTGGTCATTCCCGCTTCTTGa                                                                                                                                              <  2:771005/141‑1 (MQ=255)
gCTCTCATAATCGCAACACGTTGCTTTTCACCGCCAGATAACTGATGCGGATATACATTCAAGCGATCTTCTAAACCAATATTTTTCAAAAGTTGTATTGCTCTTGTTGAACTTTGTTGTTTGGTCATTCCCGCTTCTTGa                                                                                                                                              <  1:355980/141‑1 (MQ=255)
        aaTCGCAACACGTTGCTTTTCACCGCCAGATAACTGATGCGGATATACATTCAAGCGATCTTCTAAACCAATATTTTTCAAAAGTTGTATTGCTCTTGTTGAACTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAg                                                                                                                                      <  3:247970/141‑1 (MQ=255)
        aaTCGCAACACGTTGCTTTTCACCGCCAGATAACTGATGCGGATATACATTCAAGCGATCTTCTAAACCAATATTTTTCAAAAGTTGTATTGCTCTTGTTGAACTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAg                                                                                                                                      <  1:371791/141‑1 (MQ=255)
          tCGCAACACGTTGCTTTTCACCGCCAGATAACTGATGCGGATATACATTCAAGCGATCTTCTAAACCAATATTTTTCAAAAGTTGTATTGCTCTTGTTGAACTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAgtg                                                                                                                                    <  4:20348/141‑1 (MQ=255)
                cacGTTGCTTTTCACCGCCAGATAACTGATGCGGATATACATTCAAGCGATCTTCTAAACCAATATTTTTCAAAAGTTGTATTGCTCTTGTTGAACTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAAtt                                                                                                                              <  3:275192/141‑1 (MQ=255)
                     tGCTTTTCACCGCCAGATAACTGATGCGGATATACATTCAAGCGATCTTCTAAACCAATATTTTTCAAAAGTTGTATTGCTATTGTTGAACTTTATTGTTTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGctct                                                                                                                         <  3:371057/141‑1 (MQ=255)
                        ttttCACCGCCAGATAACTGATGCGGATATACATTCAAGCGATCTTCTAAACCAATATTTTTCAAAAGTTGTATTGCTCTTGTTGAACTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATc                                                                                                                      <  2:308920/141‑1 (MQ=255)
                        ttttCACCGCCAGATAACTGATGCGGATATACATTCAAGCGATCTTCTAAACCAATATTTTTCAAAAGTTGTATTGCTCTTGTTGAACTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATc                                                                                                                      <  2:348577/141‑1 (MQ=255)
                           tCACCGCCAGATAACTGATGCGGATATACATTCAAGCGATCTTCTAAACCAATATTTTTCAAAAGTTGTATTGCTCTTGTTGAACTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACt                                                                                                                   <  2:931125/141‑1 (MQ=255)
                           tCACCGCCAGATAACTGATGCGGATATACATTCAAGCGATCTTCTAAACCAATATTTTTCAAAAGTTGTATTGCTCTTGTTGAACTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACt                                                                                                                   <  2:656363/141‑1 (MQ=255)
                           tCACCGCCAGATAACTGATGCGGATATACATTCAAGCGATCTTCTAAACCAATATTTTTCAAAAGTTGTATTGCTCTTGTTGAACTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACt                                                                                                                   <  2:19073/141‑1 (MQ=255)
                           tCACCGCCAGATAACTGATGCGGATATACATTCAAGCGATCTTCTAAACCAATATTTTTCAAAAGTTGTATTGCTCTTGTTGAACTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACt                                                                                                                   <  1:174819/141‑1 (MQ=255)
                            cACCGCCAGATAACTGATGCGGATATACATTCAAGCGATCTTCTAAACCAATATTTTTCAAAAGTTGTATTGCTCTTGTTGAACTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACtt                                                                                                                  >  2:866820/1‑141 (MQ=255)
                             cccGCCAGATAACTGATGCGGATATACATTCAAGCGATCTTCTAAACCAGTATTTTTCAAAAGTTGTATTGCTCTTGTTGAACTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACttt                                                                                                                 <  2:3525/140‑1 (MQ=255)
                               cGCCAGATAACTGATGCGGATATACATTCAAGCGATCTTCTAAACCAATATTTTTCAAAAGTTGTATTGCTCTTGTTGAACTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTa                                                                                                               <  2:617803/141‑1 (MQ=255)
                                 ccAGATAACTGATGCGGATATACATTCAAGCGATCTTCTAAACCAATATTTTTCAAAAGTTGTATTGCTCTTGTTGAACTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTaaa                                                                                                             >  2:42545/1‑141 (MQ=255)
                                  cAGATAACTGATGCGGATATACATTCAAGCGATCTTCTAAACCAATATTTTTCAAAAGTTGTATTGCTCTTGTTGAACTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTAAAt                                                                                                            >  1:488728/1‑141 (MQ=255)
                                   aGATAACTGATGCGGATATACATTCAAGCGATCTTCTAAACCAATATTTTTCAAAAGTTGTATTGCTCTTGTTGAACTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTAAATa                                                                                                           >  4:202123/1‑141 (MQ=255)
                                         cTGATGCGGATATACATTCAAGCGATCTTCTAAACCAATATTTTTCAAAAGTTGTATTGCTCTTGTTGAACTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAAc                                                                                                     <  1:772839/141‑1 (MQ=255)
                                          tGATGCGGATATACATTCAAGCGATCTTCTAAACCAATATTTTTCAAAAGTTGTATTGCTCTTGTTGAACTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACt                                                                                                    <  1:958194/141‑1 (MQ=255)
                                            aTGCGGATATACATTCAAGCGATCTTCTAAACCAATATTTTTCAAAAGTTGTATTGCTCTTGTTGAACTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACTaa                                                                                                  <  1:1022921/141‑1 (MQ=255)
                                              gCGGATATACATTCAAGCGATCTTCTAAACCAATATTTTTCAAAAGTTGTATTGCTCTTGTTGAACTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACTAAAt                                                                                                <  1:25080/141‑1 (MQ=255)
                                                 gATATACATTCAAGCGATCTTCTAAACCAATATTTTTCAAAAGTTGTATTGCTCTTGTTGAACTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACTAAAtgtg                                                                                             <  2:1033673/141‑1 (MQ=255)
                                                   tataCATTCAAGCGATCTTCTAAACCAATATTTTTCAAAAGTTGTATTGCTCTTGTTGAACTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACTAAATGTGaa                                                                                           >  2:1021740/1‑141 (MQ=255)
                                                   tataCATTCAAGCGATCTTCTAAACCAATATTTTTCAAAAGTTGTATTGCTCTTGTTGAACTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACTAAATGTGaa                                                                                           >  2:693139/1‑141 (MQ=255)
                                                   tataCATTCAAGCGATCTTCTAAACCAATATTTTTCAAAAGTTGTATTGCTCTTGTTGAACTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACTAAATGTGaa                                                                                           >  1:308845/1‑141 (MQ=255)
                                                    ataCATTCAAGCGATCTTCTAAACCAATATTTTTCAAAAGTTGTATTGCTCTTGTTGAACTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACTAAATGTGAAg                                                                                          >  1:119652/1‑141 (MQ=255)
                                                    ataCATTCAAGCGATCTTCTAAACCAATATTTTTCAAAAGTTGTATTGCTCTTGTTGAACTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTAACACTTTTAAATAAGGAACTAAAtgaaaaa                                                                                          <  2:494463/141‑6 (MQ=255)
                                                     taCATTCAAGCGATCTTCTAAACCAATATTTTTCAAAAGTTGTATTGCTCTTGTTGAACTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACTAAATGTGAAGa                                                                                         <  1:1045032/141‑1 (MQ=255)
                                                       cATTCAAGCGATCTTCTAAACCAATATTTTTCAAAAGTTGTATTGCTCTTGTTGAACTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACTAAATATGAAGAtt                                                                                       <  2:132362/141‑1 (MQ=255)
                                                         ttCAAGCGATCTTCTAAACCAATATTTTTCAAAAGTTGTATTGCTCTTGTTGAACTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACTAAATGTGAAGATTGa                                                                                     <  3:26870/141‑1 (MQ=255)
                                                          tCAAGCGATCTTCTAAACCAATATTTTTCAATTGTTTTATTGCTCTTGTTGAACTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACTAAATGTGAAGATTGaa                                                                                    >  2:877787/1‑141 (MQ=255)
                                                          tCAAGCGATCTTCTAAACCAATATTTTTCAAAAGTTGTATTGCTCTTGTTGAACTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACTAAATGTGAAGAATGaa                                                                                    <  4:418527/141‑1 (MQ=255)
                                                            aaGCGATCTTCTAACCCAATATTTTTCAAAAGTTGTATTGCTCTTGTTGAACTTTGTTGTTTGGTCATTCCCGCTTCTTGGCCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACTAAATGTGAAGATTGaaaa                                                                                  <  1:903295/141‑1 (MQ=255)
                                                              gCGATCTTCTAAACCAATATTTTTCAAAAGTTGTATTGCTCTTGTTGAACTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACTAAATGTGAAGATTGaaaaac                                                                                <  3:349120/141‑2 (MQ=255)
                                                              gCGATCTTCTAAACCAATATTTTTCAAAAGTTGTATTGCTCTTGTTGAACTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACTAAATGTGAAGATTGAAAAAt                                                                                <  1:1043111/141‑1 (MQ=255)
                                                              gCGATCTTCTAAACCAATATTTTTCAAAAGTTGTATTGCTCTTGTTGAACTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACTAAATGTGAAGATTGAAAAAt                                                                                <  2:592267/141‑1 (MQ=255)
                                                               cGATCTTCTAAACCAATATTTTTTAAAATTTGTATTGCTCTTGTTGAACTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACTAAATGTGAAGATTGAAAAATa                                                                               >  2:423594/1‑141 (MQ=255)
                                                               cGATCTTCTAAACCAATATTTTTCAAAAGTTGTATTGCTCTTGTTGAACTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACTAAATGTGAAGATTGAAAAATa                                                                               >  2:854409/1‑141 (MQ=255)
                                                                 aTCTTCTAAACCAATATTTTTCAATTGTTGTATTGCTCTTGTTGAACTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACTAAATGTGAAGATTGAAAAATaaa                                                                             >  2:562034/1‑141 (MQ=255)
                                                                  tcttctAAAGCAATATTTTTCCAAAGTTGTATTGCTCTTGTTGAACTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGCAACTAAATGTGAAGCTTGAAAAATaaaa                                                                            >  1:956853/1‑141 (MQ=255)
                                                                  tcttctAAACCAATATTTTTCAAAAGTTGTATTGCTCTTGTTGAACTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACTAAATGTGAAGATTGAAAAATaaaa                                                                            >  3:339412/1‑141 (MQ=255)
                                                                    ttctAAACCAATATTTTTCAAAAGTTGTATTGCTCTTGTTGAACTTTTTTGTTTGGTCAATCCCTTTGCTTGGCCTTTGAGTGTCAATTGCGCTATCACTTTTAAATATGTAACTAAATGTGAAGATTGAAAAATAAAAcc                                                                          <  2:207458/141‑1 (MQ=255)
                                                                       tAAACCAATATTTTTCAAAAGTTGTATTGCTCTTGTTGAACTTTGTTGTTTGGTCATTCCAGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACTAAATGTGAAGATTGAAAAATAAAACCAat                                                                       >  2:479193/1‑141 (MQ=255)
                                                                         aaCTAATATTTTTCAAAAGTTGTATTGCTCTTGTTTATCTTTGTTGTTTGGTCATGCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTACATAAGGAACTAAAAAAAAAAAAAAAAAAATAAAACCAatat                                                                     <  2:675418/141‑1 (MQ=255)
                                                                         aaCCAATATTTTTCAAAAGTTGTATTGCTCTTGTTGAACTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACTAAATGTGAAGATTGAAAAATAAAACCAatat                                                                     >  2:497042/1‑141 (MQ=255)
                                                                         aaCCAATATTTTTCAAAAGTTGTATTGCTCTTGTTGAACTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACTAAATGTGAAGATTGAAAAATAAAACCAatat                                                                     <  1:119774/141‑1 (MQ=255)
                                                                             aaTATTTTTCAAAAGTTGTATTGCTCTTGTTGAACTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACTAAATAAAAAAATTAAAAAATAAAACCAATATCTTc                                                                 <  4:7742/141‑1 (MQ=255)
                                                                             aaTATTTTGCAAAAGTTGTATTGCTCTTGTTGAACTTTGTTGTTTGGTCATTCCCGCTTCTTGGCCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACTAAATGTGAAGATTGAAAAATAAAACCAATATCTTc                                                                 <  1:77219/141‑1 (MQ=255)
                                                                              atatTTTTCAAAAGTTGTATTGCTCTTGTTGAACTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACTAAATGTGAAGATTGAAAAATAAAACCAATATCTTcc                                                                <  1:110831/141‑1 (MQ=255)
                                                                                   tttCAAAAGTTGTATTGCTCTTGTTGAACTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACTAAATGTGAAGATTGAAAAATAAAACCAATATCTTCCAATCg                                                           >  3:318068/1‑141 (MQ=255)
                                                                                    ttCAAAAGTTGTATTGCTCTTGTTGAACTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACTAAATGTGAAGATTGAAAAATAAAACCAATATCTTCCAATCGt                                                          <  1:781882/141‑1 (MQ=255)
                                                                                    ttCAAAAGTTGTATTGCTCTTGTTGAACTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACTAAATGTGAAGATTGAAAAATAAAACCAATATCTTCCAATCGt                                                          <  2:532175/141‑1 (MQ=255)
                                                                                     tCAAAATTTTTATTGCTCTTGTTGAACTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACTAAATGTGAAGATTGAAAAATAAAACCAATATCTTCCAATCGTa                                                         >  2:588621/1‑141 (MQ=255)
                                                                                     tCAAAAGTTGTATTGCTCTTGTTGAACTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACTAAATGTGAAGATTGAAAAATAAAACCAATATCTTCCAATCGTa                                                         >  1:623118/1‑141 (MQ=255)
                                                                                     tCAAAAGTTGTATTGCTCTTGTTGAACTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACTAAATGTGAAGATTGAAAAATAAAACCAATATCTTCCAATCGTa                                                         <  1:597366/141‑1 (MQ=255)
                                                                                     tCAAAAGTTGTATTGCTCTTGTTGAACTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACTAAATGTGAAGATTGAAAAATAAAACCAATATCTTCCAATCGTa                                                         <  1:567478/141‑1 (MQ=255)
                                                                                     tCAAAAGTTGTATTGCTCTTGTTGAACTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACTAAATGTGAAGATTGAAAAATAAAACCAATATCTTCCAATCGTa                                                         >  1:230233/1‑141 (MQ=255)
                                                                                     tCAAAAGTTGTATTGCTCTTGTTGAACTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACTAAATGTGAAGATTGAAAAATAAAACCAATATCTTCCAATCGTa                                                         <  2:4416/141‑1 (MQ=255)
                                                                                       aaaaGTTGTATTGCTCTTGTTGAACTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACTAAATGTGAAGATTGAAAAATAAAACCAATATCTTCCAATCGTaaa                                                       <  1:327935/141‑1 (MQ=255)
                                                                                       aaaaGTTGTATTGCTCTTGTTGAACTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACTAAATGTGAAGATTGAAAAATAAAACCAATATCTTCCAATCGTaaa                                                       <  1:998468/141‑1 (MQ=255)
                                                                                        aaaGTTGTATTGCTCTTGTTGAACTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACTAAATGTGAAGATTGAAAAATAAAACCAATATCTTCCAATCGTAAAt                                                      <  1:62165/141‑1 (MQ=255)
                                                                                         aaGTTGTATTGCTCTTGTTGAACTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACTAAATGTGAAGATTGAAAAATAAAACCAATATCTTCCAATCGTAAATc                                                     <  2:776290/141‑1 (MQ=255)
                                                                                          ctttGTTTTGCGCTCATTGATCTTTGTTGTTTGGTCATTACCGCTTCTTGACCTACGAGTGTCAATTGCGCTATCACTTTTGTATAAGTCTCTAAATGTGAAGATTCAAAAATAAAACCAATATCTTCCAATCGTAAATCa                                                    >  4:142927/3‑141 (MQ=255)
                                                                                             tGTATTTATCTTGTGGAACTTTGTTGTTTGGTCATGCCGGGTTCTTGACCTTCGAGTGTCGATTGCTCTATCACTTTTGAATAAGGAACTAAATGTGAAGATGGTAAAATAAAACCAATATCTTCCAATCGTAAATCACTa                                                 >  4:86356/1‑141 (MQ=255)
                                                                                               tATTGCTCTTGTTGAACTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACTAAATGTGAAGATTGAAAAATAAAACCAATATCTTCCAATCGTAAATCACTAgg                                               <  2:119652/141‑1 (MQ=255)
                                                                                                aTTGCTCTTGTTGAACTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACTAAATGTGAAGATTGAAAAATAAAACCAATATCTTCCAATCGTAAATCACTAGGa                                              >  3:410210/1‑141 (MQ=255)
                                                                                                aTTGCTCTTGTTGAACTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACTAAATGTGAAGATTGAAAAATAAAACCAATATCTTCCAATCGTAAATCACTAGGa                                              >  1:761926/1‑141 (MQ=255)
                                                                                                  tGCTCTTGTTGAACTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACTAAATGTGAAGATTGAAAAATAAAACCAATATCTTCCAATCGTAAATCACTAGGACg                                            <  4:352609/141‑1 (MQ=255)
                                                                                                     tctTGTTGAACTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACTAAATGTGAAGATTGAAAAATAAAACCAATATCTTCCAATCGTAAATCACTAGGACGATg                                         <  1:620439/141‑1 (MQ=255)
                                                                                                     tctTGTTGAACTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACTAAATGTGAAGATTGAAAAATAAAACCAATATCTTCCAATCGTAAATCACTAGGACGATg                                         >  2:904199/1‑141 (MQ=255)
                                                                                                     tctTGTTGAACTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACTAAATGTGAAGATTGAAAAATAAAACCAATATCTTCCAATCGTAAATCACTAGGACGATg                                         <  1:49832/141‑1 (MQ=255)
                                                                                                     tctTGTTGAACTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACTAAATGTGAAGATTGAAAAATAAAACCAATATCTTCCAATCGTAAATCACTAGGACGATg                                         <  1:695224/141‑1 (MQ=255)
                                                                                                     tctTGTTGAACTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACTAAATGTGAAGATTGAAAAATAAAACCAATATCTTCCAATCGTAAATCACTAGGACGATg                                         <  2:230907/141‑1 (MQ=255)
                                                                                                      ctTGTTGAACTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACTAAATGTGAAGATTGAAAAATAAAACCAATATCTTCCAATCGTAAATCACTAGGACGATGc                                        <  1:22667/141‑1 (MQ=255)
                                                                                                       ttgttgAACTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACTAAATGAAAAGAATAAAAAATAAAACCAATATCTTCCAATCGTAAATCACTAGGACGATGCt                                       <  2:244758/141‑1 (MQ=255)
                                                                                                        tgttgAACTTTGTTGTTTGGTCGTTCCCACTTCTTGACCTACGAGTGTTAATTGCTCTATCACTTTTAAATAAGGAACTAAATGTGAAGATTGAAAAATAAAACCAATATCTTCCAATCGTAAATCTCTAGGACGATGCTg                                      <  4:312777/141‑1 (MQ=255)
                                                                                                        tgttgAACTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACTAAATGTGAAGATTGAAAAATAAAACCAATATCTTCCAATCGTAAATCACTAGGACGATGCTg                                      <  1:162492/141‑1 (MQ=255)
                                                                                                            gAACTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACTAAATGTGAAGATTGAAAAATAAAACCAATATCTTCCAATCGTAAATCACTAGGACGATGCTGTTTa                                  >  2:641021/1‑141 (MQ=255)
                                                                                                            gAACTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACTAAATGTGAAGATTGAAAAATAAAACCAATATCTTCCAATCGTAAATCACTAGGACGATGCTGTTTa                                  >  2:693813/1‑141 (MQ=255)
                                                                                                             aaCTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACTAAATGTGAAGATTGAAAAATAAAACCAATATCTTCCAATCGTAAATCACTAGGACGATGCTGTTTAt                                 <  3:21872/141‑1 (MQ=255)
                                                                                                             aaCTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACTAAATGTGAAGATTGAAAAATAAAACCAATATCTTCCAATCGTAAATCACTAGGACGATGCTGTTTAt                                 <  1:205389/141‑1 (MQ=255)
                                                                                                             aaCTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACTAAATGTGAAGATTGAAAAATAAAACCAATATCTTCCAATCGTAAATCACTAGGACGATGCTGTTTAt                                 <  1:42545/141‑1 (MQ=255)
                                                                                                              aCTTTGTTGTTTGGTCATTCCCGCTTCTTGGCCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACTAAATGTGAAGATTGAAAAATAAAACCAATATCTTCCAATCGCAAATCACTAGGACGATGCTGTTTATc                                >  2:334285/1‑141 (MQ=255)
                                                                                                                 ttgttgTTTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACTAAATGTGAAGATTGAAAAATAAAACCAATATCTTCCAATCGTAAATCACTAGGACGATGCTGTTTATCaaa                             <  1:429312/141‑1 (MQ=255)
                                                                                                                   gttgttTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACTAAATGTGAAGATTGAAAAATAAAACCAATATCTTCCAATCGTAAATCACTAGGACGATGCTGTTTATCAAACa                           >  1:428541/1‑141 (MQ=255)
                                                                                                                      gttTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACTAAATGTGAAGATTGAAAAATAAAACCAATATCTTCCAATCGTAAATCACTAGGACGATGCTGTTTATCAAACAATg                        >  1:76079/1‑141 (MQ=255)
                                                                                                                       tttGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACTAAATGTTAAGATTTAAAAATAAAACCAATATCTTCCAATCGTAAATCACTAGGACGATGCTGTTTAGCAAACAATgg                       <  1:268017/141‑1 (MQ=255)
                                                                                                                         tGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACTAAATGTGAAGATTGAAAAATAAAACCAATATCTTCCAATCGTAAATCACTAGGACGATGCTGTTTATCAAACAATGgcg                     >  2:259042/1‑141 (MQ=255)
                                                                                                                           gTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACTAAATGTGAAGATTGAAAAATAAAACCAATATCTTCCAATCGTAAATCACTAGGACGATGCTGTTTATCAAACAATGGCGca                   <  2:242521/141‑1 (MQ=255)
                                                                                                                            tCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACTAAATGTGAAGATTGAAAAATAAAACCAATATCTTCCAATCGTAAATCACTAGGACGATGCTGTTTATCAAACAATGGCGcat                  <  2:571064/141‑1 (MQ=255)
                                                                                                                             cATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACTAAATGTGAAGATTGAAAAATAAAACCAATATCTTCCAATCGTAAATCACTAGGACGATGCTGTTTATCAAACAATGGCGcatc                 <  1:1497/141‑1 (MQ=255)
                                                                                                                               ttCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACTAAATGTGAAGATTGAAAAATAAAACCAATATCTTCCAATCGTAAATCACTAGGACGATGCTGTTTATCAAACAATGGCGcatcat               >  1:578504/1‑141 (MQ=255)
                                                                                                                                tCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACTAAATGTGAAGATTGACAAATAACACAAATATCTTCCAATCGTAAATCACTAGGACGATGCTGTTTATCAAACAATGGCGCATCAtt              >  1:917773/1‑141 (MQ=255)
                                                                                                                                tCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACTAAATGTGAAGATTGAAAAATAAAACCAATATCTTCCAATCGTAAATCACTAGGACGATGCTGTTTATCAAACAATGGCGCATCAtt              >  2:230033/1‑141 (MQ=255)
                                                                                                                                     cttcttGACCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACTAAATGTGAAGATTGAAAAATAAAACCAATATCTTCCAATCGTAAATCACTAGGACGATGCTGTTTATCAAACAATGGCGCATCATTGTaaa         <  4:217358/141‑1 (MQ=255)
                                                                                                                                       tcttGGCCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACTAAATGTGAAGATTGAAAAATAAAACCAATATCTTCCAATCGCAAATCACTAGGACGATGCTGTTTATCAAACAATGGTGCATCATTGTAAAGc       >  1:1041266/1‑141 (MQ=255)
                                                                                                                                            aCCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACTAAATGTGAAGATTGAAAAATAAAACCAATATCTTCCAATCGTAAATCACTAGGACGATGCTGTTTATCAAACAATGGCGCATCATTGTAAAGCACTGt  <  2:562013/141‑1 (MQ=255)
                                                                                                                                            aCCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACTAAATGTGAAGATTGAAAAATAAAACCAATAACTTCCAATCGTAAATCACTAGGACGATGCTGTTTATCAAACAATGGCGCATCATTGTAAAGCACTGt  <  1:78393/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                            
GCTCTCATAATCGCAACACGTTGCTTTTCACCGCCAGATAACTGATGCGGATATACATTCAAGCGATCTTCTAAACCAATATTTTTCAAAAGTTGTATTGCTCTTGTTGAACTTTGTTGTTTGGTCATTCCCGCTTCTTGACCTACGAGTGTCAATTGCTCTATCACTTTTAAATAAGGAACTAAATGTGAAGATTGAAAAATAAAACCAATATCTTCCAATCGTAAATCACTAGGACGATGCTGTTTATCAAACAATGGCGCATCATTGTAAAGCACTGT  >  USA300TCH1516_ALE/2479386‑2479666

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: