Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 2,525,787 A→T 10.0% I136I (ATT→ATA ytmI ← putative N‑acetyltransferase YtmI

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,525,7870AT10.0% 355.6 / 4.1 126I136I (ATT→ATAytmIputative N‑acetyltransferase YtmI
Reads supporting (aligned to +/- strand):  ref base A (51/58);  new base T (13/2);  total (64/62)
Fisher's exact test for biased strand distribution p-value = 4.75e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 131 total aligned reads displayed.

ATATTATTATCTTAAAATGGTGGTTGGCCTAATATTGTTTCGTCAAAGCGCTCGGGTATCAATACTTTGCGCATGATCACACCTAAATCGCCATCATCATTTTCATGTTCGCTGTATATTTCATAACCTCTTTTTTCATAAATTTTAAGTAACCACGGATGCAATCTTGCAGATGTACCTAAAGTAACTGCCGCTGACTTTAACGTATCTCGCAAAAATGCTTCTTCAACATAAGTAAGTAATTGGCTACCATAGCCTTTCCCTTCATACTCAGGATTTGT  >  USA300TCH1516_ALE/2525647‑2525927
                                                                                                                                            |                                                                                                                                            
atatTATTATCTTAAAATGGTGGTTGGCCTAATATTGTTTCGTCAAAGCGCTCGGGTATCAATACTTTGCGCATGATCACACCTAAATCGCCATCATCATTTTCATGTTCGCTGTATATTTCATAACCTCTTTTTTCATaa                                                                                                                                              >  3:51990/1‑141 (MQ=255)
      ttatCTTAAAATGGTGGTTGGCCTAATATTGTTTCGTCAAAGCGCTCGGGTATCAATACTTTGCGCATGATCACACCTAAATCGCCATCATCATTTTCATGTTCGCTGTATATTTCATAACCTCTTTTTTCATAAATTTTa                                                                                                                                        >  2:892510/1‑141 (MQ=255)
        atCTTAAAATGGTGGTTGGCCTAATATTGTTTCGTCAAAGCGCTCGGGTATCAATACTTTGCGCATGATCACACCTAAATCGCCATCATCATTTTCATGTTCGCTGTATATTTCATAACCTCTTTTTTCATAAATTTTaat                                                                                                                                      >  2:837976/1‑140 (MQ=255)
        atCTTAAAATGGTGGTTGGCCTAATATTGTTTCGTCAAAGCGCTCGGGTATCAATACTTTGCGCATGATCACACCTAAATCGCCATCATCATTTTCATGTTCGCTGTATATTTCATAACCTCTTTTTTCATAAATTTTaat                                                                                                                                      >  2:628103/1‑140 (MQ=255)
        atCTTAAAATGGTGGTTGGCCTAATATTGTTTCGTCAAAGCGCTCGGGTATCAATACTTTGCGCATGATCACACCTAAATCGCCATCATCATTTTCATGTTCGCTGTATATTTCATAACCTCTTTTTTCATAAATTTTaat                                                                                                                                      >  2:49794/1‑140 (MQ=255)
        atCTTAAAATGGTGGTTGGCCTAATATTGTTTCGTCAAAGCGCTCGGGTATCAATACTTTGCGCATGATCACACCTAAATCGCCATCATCATTTTCATGTTCGCTGTATATTTCATAACCTCTTTTTTCATAAATTTTAAg                                                                                                                                      <  1:786993/141‑1 (MQ=255)
         tCTTAAAATGGTGGTTGGCCTAATATTGTTTCGTCAAAGCGCTCGGGTATCAATACTTTGCGCATGATCACACCTAATTCGCCATCATCATTTTCATGTTCGCTGTATATTTCATAACCTCTTTTTTCATAAATTTTAAGt                                                                                                                                     <  2:142708/141‑1 (MQ=255)
             aaaaTGGTGGTTGGCCTAATATTGTTTCGTCAAAGCGCTCGGGTATCAATACTTTGCGCATGATCACACCTAAATCGCCATCATCATTTTCATGTTCGCTGTATATTTCATAACCTCTTTTTTCATAATTTTTAAGTAAcc                                                                                                                                 >  4:197467/1‑141 (MQ=255)
                  ggtggtTGGCCTAATATTGTTTCGTCAAAGCGCTCGGGTATCAATACTTTGCGCATGATCACACCTAAATCGCCATCATCATTTTCATGTTCGCTGTATATTTCATAACCTCTTTTTTCATAAATTTTAAGTAACCACGGa                                                                                                                            >  1:835825/1‑141 (MQ=255)
                    tggtTGGCCTAATATTGTTTCGTCAAAGCGCTCGGGTATCAATACTTTGCGCATGATCACACCTAAATCGCCATCATCATTTTCATGTTCGCTGTATATTTCATAACCTCTTTTTTCATAAATTTTAAGTAACCACGGATg                                                                                                                          >  1:408379/1‑141 (MQ=255)
                     ggtTGGCCTAATATTGTTTCGTCAAAGCGCTCGGGTATCAATACTTTGCGCATGATCACACCTCAATCGCCCTCATCATTTTCATGTTCGCTGTATATTTCATAACCTCTTTTTTCATAAATTTTAAGTAACCACGGATgg                                                                                                                         <  1:1029735/141‑2 (MQ=255)
                     ggtTGGCCTAATATTGTTTCGTCAAAGCGCTCGGGTATCAATACTTTGCGCATGATCACACCTAAATTGCCATCATCATTTTCATGTTCGCTGTATATTTCATAACCTCTTTTTTCATAAATTTTAAGTAACCACGGATGc                                                                                                                         <  1:995748/141‑1 (MQ=255)
                     ggtTGGCCTAATATTGTTTCGTCAAAGCGCTCGGGTATCAATACTTTGCGCATGATCACACCTAAATCGCCATCATCATTTTCATGTTCGCTGTATATTTCATAACCTCTTTTTTCATAAATTTTAAGTAACCACGGATGc                                                                                                                         >  2:492617/1‑141 (MQ=255)
                     ggtTGGCCTAATATTGTTTCGTCAAAGCGCTCGGGTATCAATACTTTGCGCATGATCACACCTAAATCGCCATCATCATTTTCATGTTCGCTGTATATTTCATAACCTCTTTTTTCATAAATTTTAAGTAACCACGGATGc                                                                                                                         >  2:605071/1‑141 (MQ=255)
                     ggtTGGCCTAATATTGTTTCGTCAAAGCGCTCGGGTATCAATACTTTGCGCATGATCACACCTAAATCGCCATCATCATTTTCATGTTCGCTGTATATTTCATAACCTCTTTTTTCATAAATTTTAAGTAACCACGGATGc                                                                                                                         <  2:847600/141‑1 (MQ=255)
                     ggtTGGCCTAATATTGTTTCGTCAAAGCGCTCGGGTATCAATACTTTGCGCATGATCACACCTAAATCGCCATCATCATTTTCATGTTCGCTGTATATTTCATAACCTCTTTTTTCATAAATTTTAAGTAACCACGGATGc                                                                                                                         >  4:371912/1‑141 (MQ=255)
                     ggtTGGCCTAATATTGTTTCGTCAAAGCGCTCGGGTATCAATACTTTGCGCATGATCACACCTAAATCGCCATCATCATTTGCATGTTCGCTGTATATTTCATAACCTCTTTTTTCATAAATTTTAAGTAACCACGGATGc                                                                                                                         <  1:173008/141‑1 (MQ=255)
                     ggctGGCCTACTACCCTTGCGTCTAACCGCTCGCGTGCCAATACTTTGCTTATGATCACACCTAAATCGCCATCATCATTTTCATGTTCGCTGTATATTTCATAACCTCTTTTTTCATAAATTTTAAGTAACCACGGATGc                                                                                                                         <  3:359583/138‑1 (MQ=255)
                        tGGCCTAATATTGTTTCGTCAAAGCGCTCGGGTATCAATACTTTGCGCATGATCACACCTAAATCGCCATCATCATTTTCATGTTCGCTGTATATTTCATAACCTCTTTTTTCATAAATTTTAAGTAACCACGGATGCAAt                                                                                                                      >  3:392446/1‑141 (MQ=255)
                               atatTGTTTCGTCAAAGCGCTCGGGTATCAATACTTTGCGCATGATCACACCTAAATCGCCATCATCATTTTCATGTTCGCTGTATATTTCATAACCTCTTTTTTCATAAATTTTAAGTAACCACGGATGCAATCTTGCAg                                                                                                               <  1:465887/141‑1 (MQ=255)
                               atatTGTTTCGTCAAAGCGCTCGGGTATCAATACTTTGCGCATGATCACACCTAAATCGCCATCATCATTTTCATGTTCGCTGTATATTTCATAACCTCTTTTTTCATAAATTTTAAGTAACCACGGATGCAATCTTGCAg                                                                                                               >  1:49085/1‑141 (MQ=255)
                                  ttGTTTCGTCAAAGCGCTCGGGTATCAATACTTTGCGCATGATCACACCTAAATCGCCATCATCATTTTCATGTTCGCTGTATATTTCATAACCTCTTTTTTCATAAATTTTAAGTAACCACGGATGCAATCTTGCAGATg                                                                                                            >  3:318167/1‑141 (MQ=255)
                                    gTTTCGTCAAAGCGCTCGGGTATCAATACTTTGCGCATGATCACACCTAAATCGCCATCATCATTTTCATGTTCGCTGTATATTTCATAACCTCTTTTTTCATAAATTTTAAGTAACCACGGATGCAATCTTGCAGATGTa                                                                                                          >  1:604812/1‑141 (MQ=255)
                                    gTTTCGTCAAAGCGCTCGGGTATCAATACTTTGCGCATGATCACACCTAAATCGCCATCATCATTTTCATGTTCGCTGTATATTTCATAACCTCTTTTTTCATAAATTTTAAGTAACCACGGATGCAATCTTGCAGATGTa                                                                                                          >  4:164139/1‑141 (MQ=255)
                                        cGTCAAAGCGCTCGGGTATCAATACTTTGCGCATGATCACACCTAAATCGCCATCATCATTTTCATGTTCGCCGTATATTTCATAACCTCTTTTTTCATAAATTTTAAGTAACCACGGATGCAATCTTGCAGATGTACCTa                                                                                                      <  1:274688/141‑1 (MQ=255)
                                         gTCAAAGCGCTCGGGTATCAATACTTTGCGCATGATCACACCTAAATCGCCATCATCATTTTCATGTTCGCTGTATATTTCATAACCTCTTTTTTCATAAATTTTAAGTAACCACGGATGCAATCTTGCAGATGTACCTaa                                                                                                     <  2:660245/141‑1 (MQ=255)
                                          tCAAAACGCTCGGGTATCAATACTTTGCGCATGATCACACCTAAATCGCCATCATCATTTTCATGTTCGCTGTATATTTCATAACCTCTTTTTTCATAAATTTTAAGTAACCACGGATGCAATCTTGCAGATGTACCTaaa                                                                                                    >  2:815034/1‑141 (MQ=255)
                                            aaaGCGCTCGGGTATCAATACTTTGCGCATGATCACACCTAAATCGCCATCATCATTTTCATGTTCGCTGTATATTTCATAACCTCTTTTTTCATAAATTTTAAGTAACCACGGATGCAATCTTGCAGATGTACCTAAAGt                                                                                                  <  4:377972/141‑1 (MQ=255)
                                              aGCGCTCGGGTATCAATACTTTGCGCATGATCACACCTAAATCGCCATCATCATTTTCATGTTCGCTGTATATTTCATAACCTCTTTTTTCATAAATTTTAAGTAACCACGGATGCAATCTTGCAGATGTACCTAAAGTaa                                                                                                >  2:451845/1‑141 (MQ=255)
                                              aGCGCTCGGGTATCAATACTTTGCGCATGATCACACCTAAATCGCCATCATCATTTTCATGTTCGCTGTATATTTCATAACCTCTTTTTTCATAAATTTTAAGTAACCACGGATGCAATCTTGCAGATGTACCTAAAGTaa                                                                                                >  1:1078016/1‑141 (MQ=255)
                                               gcgcTCGGGTATCAATACTTTGCGCATGATCACACCTAAATCTCCATCATCATTTTCATGTTCGCTGTATATTTCATAACCTCTTTTTTCATAAATTTTAAGTAACCACGGATGCAATCTTGCAGATGTACCTAAAGTAAc                                                                                               <  3:254770/141‑1 (MQ=255)
                                                   tCGGGTATCAATACTTTGCGCATGATCACACCTAAATCGCCATCATCATTTTCATGTTCGCTGTATATTTCATAACGTCTTTTTTCATAAATTTTAAGTAACCACGGATGCAATCTTGCAGATGTACCTAAAGTAACTGcc                                                                                           >  4:437784/1‑141 (MQ=255)
                                                   tCGGGTATCAATACTTTGCGCATGATCACACCTAAATCGCCATCATCATTTTCATGTTCGCTGTATATTTCATAACCTCTTTTTTTATATTTTTTTTTTTACCACGGATGCAATCTTGCAGATGTACCTAAAGTAACTGcc                                                                                           >  2:231181/1‑141 (MQ=255)
                                                     gggTATCAATACTTTGCGCATGATCACACCTAAATCGCCATCATCATTTTCATGTTCGCTGTATATTTCATAACCTCTTTTTTCATTTATTTTAAGTAACCACGGATGCAATCTTGCAGATGTACCTAAAGTAACTGCCGc                                                                                         >  2:249021/1‑141 (MQ=255)
                                                      ggTATCAATACTTTGCGCATGATCACACCTAAATCGCCATCATCATTTTCATGTTCGCTGTATATTTCATAACCTCTTTTTTCATAAATTTTAAGTAACCACGGATGCAATCTTGCAGATGTACCTAAAGTAACTGCCGCt                                                                                        <  4:13873/141‑1 (MQ=255)
                                                        tATTAATACTTTGCGCATGATCACACCTAAATCGCCATCATCATTTTCATGTTCGCTGTATATTTCATAACCTCTTTTTTCATAAATTTTAAGTAACCACGGATGCAATCTTGCAGATGTACCTAAAGTAACTGCCGCTGa                                                                                      <  1:512775/141‑1 (MQ=255)
                                                        tATCAATACTTTGCGCATGATCACACCTAAATCGCCATCATCATTTTCATGTTCGCTGTATATTTCATAACCTCTTTTTTCATAAATTTTAAGTAACCGCGGATGCAATCTTGCAGATGTACCTAAAGTAACTGCCGCTGa                                                                                      <  1:935193/141‑1 (MQ=255)
                                                        tATCAATACTTTGCGCATGATCACACCTAAATCGCCATCATCATTTTCATGTTCGCTGTATATTTCATAACCTCTTTTTTCATAAATTTTAAGTAACCACGGATGCAATCTTGCAGATGTACCTAAAGTAACTGCCGCTGa                                                                                      <  2:287017/141‑1 (MQ=255)
                                                         aTCAATACTTTGCGCATGATCACACCTAAATCGCCATCCTCATTTTCCAGAACGGTGTGTATCGCATAACCTCTATTTGCATAAATTTTAAGTAACCACGGATGCAATCTTGCAGATGTACCTAAAGTAACTGCCGCTGAc                                                                                     <  1:486337/141‑1 (MQ=255)
                                                          tCAATACTTTGCGCATGATCACACCTAAATCGCCATCATCATTTTCATGTTCGCTGTATATTTCATAACCTCTTTTTTCATAAATTTTAAGTAACCACGGATGCAATCTTGCAGATGTACCTAAAGTAACTGCCGCTGACt                                                                                    <  4:177068/141‑1 (MQ=255)
                                                            aaTACTTTGCGCATGATCACACCTAAATCGCCATCATCATTTTCATGTTCGCTGTATATTTCATAACCTCTTTTTTCATAAATTTTAAGTAACCACGGATGCAATCTTGCAGATGTACCTAAAGTAACTGCCGCTGACttt                                                                                  <  1:417615/141‑1 (MQ=255)
                                                             aTACTTTGGGGATGATCACACCTAAATCGCCATCATCATTTTCAGGTTCGCTGTATATTTCATAAACTATTTTTTCATATATTTTAAGTAACCACGGATGCAATCTTGCAGATGTACCTAAGGTAACTGCCGCTGACTTTa                                                                                 <  4:4361/141‑1 (MQ=255)
                                                                  ttGCGCATGATCACACCTAAATCGCCATCATCATTTTCATGTTCGCTGTATATTTCATAACCTCTTTTTTCATAAATTTTAAGTAACCACGGATGCAATCTTGCAGATGTACCTAAAGTAACTGCCGCTGACTTTAACGTa                                                                            >  2:993768/1‑141 (MQ=255)
                                                                    gcgcATGATCACACCTAAATCGCCATCATCATTTTCATGTTCGCTGTATATTTCATAACCTCTTTTTTCATAAATTTTAAGTAACCACGGATGCAATCTTGCAGATGTACCTAAAGTAACTGCCGCTGACTTTAACGTAtc                                                                          >  2:425673/1‑141 (MQ=255)
                                                                    gcgcATGATCACACCTAAATCGCCATCATCATTTTCATGTTCGCTGTATATTTCATAACCTCTTTTTTCATAAATTTTAAGTAACCACGGATGCAATCTTGCAGATGTACCTAAAGTAACTGCCGCTGACTTTAACGAAtc                                                                          <  4:264687/141‑1 (MQ=255)
                                                                       cATGATCACACCTAAATCGCCATCATCATTTTCATGTTCGCTGTATATTTCATAACCTCTTTTTTCATAAATTTTAAGTAACCACGGATGCAATCTTGCAGATGTACCTAAAGTAACTGCCGCTGACTTTAACGTATCTCg                                                                       <  1:339991/141‑1 (MQ=255)
                                                                        aTGATCACACCTAAATCGCCATCATCATTTTCATGTTCGCTGTATATTTCATAACCTCTTTTTTCATAAATTTTAAGTAACCACGGATGCAATCTTGCAGATGTACCTAAAGTAACTGCCGCTGACTTTAACGTATCTCGc                                                                      <  3:261510/141‑1 (MQ=255)
                                                                          gATCACACCTAAATCGCCATCATCATTTTCATGTTCGCTGTATATTTCATAACCTCTTTTTTCATAAATTTTAAGTAACCACGGATGCAATCTTGCAGATGTACCTAAAGTAACTGCCGCTGACTTTAACGTATCTCGCaa                                                                    >  2:805964/1‑141 (MQ=255)
                                                                          gATCACACCTAAATCGCCATCATCATTTTCATGTTCGCTGTATATTTCATAACCTCTTTTTTCATAAATTTTAAGTAACCACGGATGCAATCTTGCAGATGTACCTAAAGTAACTGCCGCTGACTTTAACGTATCTCGCaa                                                                    >  1:361089/1‑141 (MQ=255)
                                                                            tCACACCTAAATCGCCATCATCATTTTCATGTTCGCTGTATATTTCATAACCTTCTTTATCTTAAATTTTAAGTAACCACGGATGCAATCTTGCAGATGTACCTAAAGTAACTGCCGCTGACTTTAACGTATCTCGCaaaa                                                                  <  1:342592/141‑1 (MQ=255)
                                                                            tCACACCTAAATCGCCATCATCATTTTCATGTTCGCTGTATATTTCATAACCTCTTTTTTCATAAATTTTAAGTAACCACGGATGCAATCTTGCAGATGTACCTAAAGTAACTGCCGCTGACTTTAAAGTATATAGAaaaa                                                                  <  2:117824/141‑1 (MQ=255)
                                                                             cacaCCTAAATCGCCATCATCATTTTCATGTTCGCTGTATATTTCATAACCTCTTTTTTCATAAATTTTAAGTAACCACGGATGCAATCTTGCAGATGTACCTAAAGTAACTGCCGCTGACTTTAACGTATCTCGCaaaaa                                                                 >  2:704471/1‑141 (MQ=255)
                                                                               cacCTAAATCGCCATCATCATTTTCATGTTCGCTGTATATTTCATAACCTCTTTTTTCATATTTTTTAAGTAACCACGGATGCAATCTTGCAGATGTACCTAAAGTAACTGCCGCTGACTTTAACGTATCTCGCAAAAAtt                                                               >  2:109575/1‑140 (MQ=255)
                                                                               cacCTAAATCGCCATCATCATTTTCATGTTCGCTGTATATTTCATAACCTCTTTTTTCATAAATTTTAAGTAACCACGGATGCAATCTTGCAGATGTACCTAAAGTAACTGCCGCTGACTTTAACGTATCTCGCAAAAATg                                                               >  2:177285/1‑141 (MQ=255)
                                                                               cacCTAAATCGCCATCATCATTTTCATGTTCGCTGTATATTTCATAACCTCTTTTTTCATAAATTTTAAGTAACCACGGATGCAATCTTGCAGATGTACCTAAAGTAACTGCCGCTGACTTTAACGTATCTCGCAAAAATg                                                               <  1:143924/141‑1 (MQ=255)
                                                                                 ccTAAATCGCCATCTTCATTTTCATGTTCGCTGTATATTTCATATCCTCTTTTTTCATATATTTTAAGTAACCACGGATGCAATCTTGCAGATGTACCTAAAGCAACTGCCGCTGACTTTAACGTATCTCGCAAAGATGct                                                             <  2:1060104/141‑1 (MQ=255)
                                                                                       tCGCCATCATCATTTTCATGTTCGCTGTATATTTCATAACCTCTTTTTTCATAAATTTTAAGTAACCACGGATGCAATCTTGCAGATGTACCTAAAGTAACTGCCGCTGACTTTAACGTATCTCGCAAAAATGCTTCTTCa                                                       <  1:636233/141‑1 (MQ=255)
                                                                                        cGCCATCATCATTTTCATGTTCGCTGTATATTTCATAACCTCTTTTTTCATAAATGTTAAGTAACCACGGATGCAATCTTGCAGATGTACCTAAAGTAACTGCCGCTGACTTTAACGAAAAAAACAAAAATGCTTCTTCaa                                                      <  2:131699/141‑1 (MQ=255)
                                                                                           catcatcatTTTCATGTTCGCTGTATATTTCATAACCTCTTTTTTTTTTTTTTTTAAGTAACCACTGATGCAATCTTTTAGATGTACCTATATTAACTTCCGCTGACTTTATCTTATCTCGCATAAATGCTTCTTCACCAt                                                   >  2:484366/1‑141 (MQ=255)
                                                                                           catcatcatTTTCATGTTCGCTGTATATTTCATAACCTCTTTTTTCATAAATTTTAAGTAACCACGGATGCAATCTTGCAGATGTACCTAAAGTAACTGCCGCTGACTCTAACGTATCTCGCAAAAATGCTTCTTCAACAt                                                   >  1:1051974/1‑141 (MQ=255)
                                                                                            atcatcatTTTCATGTTCGCTGTATATTTCATAACCTCTTTTTTCATAAATTTTAAGTAACCACGGATGCAATCTTGCAGATGTACCTAAAGTAACTGCCGCTGACTATAACGTATCTCGCAAAAATGCTTCTTCAACAta                                                  <  2:75308/141‑1 (MQ=255)
                                                                                              catcatTTTCATGTTCGCTGTATATTTCATAACCTCTTTTTTCATAAATTTTAAGTAACCACCGATGCAATCTTGCAGATGTACCTAAAGTAACTGCCGCTGACTTTAACGTATCTCGCAAAAATGCTTCTTCAACAtaag                                                <  2:846244/141‑1 (MQ=255)
                                                                                              cataatTTTCATTTTCGATTTATAATTCATAACCTGTTTTTTCATAGATTATAAGTAACCACTGATGCAATCTTGCAGATCTACCTAAGGTAACTGCCACTGACTTTAAAATAAAACAAAAAAATGCTTCTTCAACAtaag                                                <  4:194060/141‑1 (MQ=255)
                                                                                                tcatTTTCATGTTCGCTGTATATTTCATAACCTCTTTTTTTTTTTTTTTTTTTTAACCACGGATGCAATCTTGCAGATGTACCTAAAGTAACTGCCGCTGACTTTAACGTATCTCGCACAAATGCTTCTTCAACAtaagta                                              >  3:428206/1‑141 (MQ=255)
                                                                                                   ttttCATGTTCGCTGTATATTTCATAACCTCTTTTTTTTTTAATTTTAAGTAACCACGGATGCAATCTTGCAGATGTACCTAAAGTAACTGCCGCTGACTTTAACGTATTTCGCAAAAATGCTTCTTCAACATaagtaagt                                           >  2:894470/1‑141 (MQ=255)
                                                                                                        aagTTCGCTGCATATTCCATAACCACTTTATTAATAAATTTTAAATAACCACGGATGCAATCTATCAGATGTAACTAAAATAACTGCCGCTGACCTTAACTTATCTCGCAAAAATGCTTCTTCAACATACGTAAGTAAttt                                      >  2:884113/3‑140 (MQ=255)
                                                                                                        aTGTTCGCTGTATATTTCATAACCTCTTTTTTTTTTTTTTTTAAGTCACCACGGATGCAATCTTGCAGATGTACCTAAAGTAACTGCCGCTGACTTTAACGTATCTCGCAAAAATTCTTCTTCAACATAAATAAGTAAttt                                      >  4:447260/1‑140 (MQ=255)
                                                                                                         tGTTCGCTGTATATTTCATAACCTCTTTTTTCATAAATTTTAAGTAACCACGGATGCAATCTTGCAGATGTACCTAAAGTAACTGCCGCTGACTTTAACGTATCTCGCAAAAATGCTTCTTCAACATAAGTAAGTAATTgg                                     >  2:966736/1‑141 (MQ=255)
                                                                                                          gTTCGCTGTATATTTCATAACCTCTTTTTTTTTTTTTTTTTAATAACCACACATGCTATCTTGTAGATGTACCTAAATTAACTTCCGCTGACTTTAACATATCTCTCTAAATTTTTTCTTAAATTTAATTTAGTAAtcttc                                    >  2:1038548/1‑137 (MQ=255)
                                                                                                          gTTCGCTGTATATTTCATAACCTCTTTTTTCATAAATTTTAAGTAACCACGGATGCAATCTTGCAGATGTACCTAAAGTAACTGCCGCTGACTTTAACGTATCTCGCAAAAATGCTTCTTCAACATAAGTAAGTAATTGGc                                    >  3:290716/1‑141 (MQ=255)
                                                                                                          gTTCGCTGTATATTTCATAACCTCTTCTTTCATAAATTTTAAGTAACCACGGATGCAATCTTGCAGATGTACCTAAAGTAACTGCCGCTGACTTTAAAGTAACACGCAAAAATGCTTCTTCAACATAAGTAAGTAATTGGc                                    <  2:860483/141‑1 (MQ=255)
                                                                                                           ttCGCTGTATATTTCATACCCTCCTTTTTCATACATTTTAAGTAGTCTCGGATGCTATCTTGCAGATGTACCTATAGTATCGGCCGCTGACTTTAACGTATCTCGCTAAAATGCTTCTTCAACATAAGTAAGTAATTGGCt                                   <  2:438880/141‑1 (MQ=255)
                                                                                                           ttCGCTGTATATTTCATAACCTCTTTTTTCATAAATTTTAAGTAACCACGGATGCAATCTTTCAGATGTACCTAAAGTAACTGCAGCTGACTTTAACGTATGTCGCAAAAATGCTTCTTCAACATAAGTAAGTAATTGGCt                                   >  2:715388/1‑141 (MQ=255)
                                                                                                             cGCTGTATATTTCATAACCTCTTTTTTCATAAATTTTAAGTAACCACGGATGCAATCTTGCAGATGTACCTAAAGTAACTGCCGCTGACTTTAACGTATCTCGCAAAAATGCTTCTTCAACATAAGTAAGTAATTGGCTAc                                 >  1:901478/1‑141 (MQ=255)
                                                                                                              gCTGTATATTTCATAACCTCTTTTTTTTTTTTTTTTTTGTAACCACGGATGCAATCTTGCAGATGTACCTAAAGTAACTGCCGCTGACTTTAACGTCTCTCGCAAAAATGCTTCTTCAACATAAGTAAGTAATTGGCTAcc                                >  2:615385/1‑141 (MQ=255)
                                                                                                               cTGTATATTTCATAACCTCTTTTTTCATAAATTTTAAGTAACCACGGATGCAATCTTGCAGATGTACCTAAAGTAACTGCCGCTGACTTTAACGTATCTCGCAAAAATGCTTCTTCAACATAAGTAAGTAATTGGCTatgg                               <  1:382741/141‑4 (MQ=255)
                                                                                                                tGTATATTTCATAACCTCTTTTTTCATAAATTTTAAGTAACCACGGATGCAATCTTGCAGATGTACCTAAAGTAACTGCCGCTGACTTTAACGTATCTCGCAAAAATGCTTCTTCAACATAAGTAAGTAATTGGCTACCAt                              <  4:139043/141‑1 (MQ=255)
                                                                                                                   atatTTCATAACCTCTTTTTTCATAAATTTTAAGTAACCACGGATGCAATCTTGCAGATGTACCTAAAGTAACTGCCGCTGACTTTAACGTATCTCGCAAAAATGCTTCTTCAACATAAGTAAGTAATTGGCTACCATAGc                           >  1:436422/1‑141 (MQ=255)
                                                                                                                   atatTTCATAACCTCTTTTTTCATAAATTTTAAGTAACCACGGATGCAATCTTGCAGATGTACCTAAAGTAACTGCCGCTGACTTTAACGTATCTCGCAAAAATGCTTCTTCAACATAAGTAAGTAATTGGCTACCATAGc                           <  1:993768/141‑1 (MQ=255)
                                                                                                                   atatTTCATAACCGCTTTTTTCATAAATTTTAAGTAACCACGGATGCAATCTTGCAGATGTACCTAAAGTAACTGCCGCTGACTTTAACGTATCTCGCAAAAATGCTTCTTCAACATAAGTAAGTAATTGGCTACCATAGc                           <  2:588688/141‑1 (MQ=255)
                                                                                                                    tatTTCATAACCTCTTTTTTCATAAATTTTAAGTAACCACGGATGCAATCTTGCAGATGTACCTAAAGTAACTGCCGCTGACTTTAACGTATCTCGCAAAAATGCTTCTTCAACATAAGTAAGTAATTGGCTACCATAGcc                          <  2:168142/141‑1 (MQ=255)
                                                                                                                    tatTTCATAACCTCTTTTTTCATAAATTTTAAGTAACCACGGATGCAATCTTGCAGATGTACCTAAAGTAACTGCCGCTGACTTTAACGTATCTCGCAAAAATGCTTCTTCAACATAAGTAAGTAATTGGCTACCATAGcc                          <  2:604812/141‑1 (MQ=255)
                                                                                                                    tatTTCATAACCTCTTTTTTCATAAATTTTAAGTAACCACGGATGCAATCTTGCAGATGTACCTAAAGTAACTGCCGCTGACTTTAACGTATCTCGCAAAAATGCTTCTTCAACATAAGTAAGTAATTGGCTACCATAGcc                          >  3:83967/1‑141 (MQ=255)
                                                                                                                     atTTCATAACCTCTTTTTTCATAAATTTTAAGTAACCACGGATGCAATCTTGCAGATGTACCTAAAGTAACTGCCGCTGACTTTAACGTATAACGCAAAAATGCTTCTTCAACATAAGTAAGTAATTGGCTACCATAGCCt                         <  4:384361/141‑1 (MQ=255)
                                                                                                                       ttCATAACCTCTTTTTTCATAAATTTTAAGTAACCACGGATGCAATCTTGCAGATGTACCTAAAGTAACTGCCGCTGACTTTAACGTATCTCGCAAAAATGCTTCTTCAACATAAGTAAGTAATTGGCTACCATAGCCttt                       >  2:92573/1‑141 (MQ=255)
                                                                                                                       ttCATAACCTCTTTTTTCATAAATTTTAAGTAACCACGGATGCAATCTTGCAGATGTACCTAAAGTAACTGCCGCTGACTTTAACGTATCTCGCAAAAATGCTTCTTCAACATAAGTAAGTAATTGGCTACCATAGCCttt                       >  1:242601/1‑141 (MQ=255)
                                                                                                                        tCATAACCTCTTTTTTTTTTTTTTTTTAGTAACTACGGATGCAATCTTGCAGATGTACCTAAAGTAACTGCCGCTGACTTTAACGTATCTCGCATAAATGCTTCTTCAACATAAGTATGTTATTTTCTACCATAGCCTTTc                      >  2:714070/1‑141 (MQ=255)
                                                                                                                           tAACCTCTTTTTTTTTTTTTTTTAAGTAACCACGGATGCAATCTTTCATATGTACCTAAAGTAACTGCCGCTGACTTTAACGTATCTCGCAAAAATTCTTCTTCAACATTTGTAATTATTTTTCTACCATAGCCTTTCCCt                   >  4:288238/1‑141 (MQ=255)
                                                                                                                           tAACCTCTTTTTTCATAAATTTTAAGTAACCACGGATGCAATCTTGCAGATGTACCTAAAGTAACTGCCGCTGACTTTAACGTATCTCGCAAAAATGCTTCTTCAACATAAGTAAGTAATTGGCTACCATAGCCTTTCCCt                   >  1:768960/1‑141 (MQ=255)
                                                                                                                           tAACCTCTTTTTTCATAAATTTTAAGTAACCACGGATGCAATCTTGCAGATGTACCTAAAGTAACTGCCGCTGACTTTAACGTATCTCGCAAAAATGCTTCTTCAACATAAGTAAGTAATTGGCTACCATAGCCTTTCCCt                   <  3:394512/141‑1 (MQ=255)
                                                                                                                           tAACCTCTTTTTTCATAAATTTTAAGTAACCACGGATGCAATCTTGCAGATGTACCTAAAGTAACTGCCGCTGACTTTAAAAAAAAAAAAAAAAATACTTCTTCAACATAAGTAAGTAATTGGCTACCATAGCCTTTCCCt                   <  4:282946/141‑1 (MQ=255)
                                                                                                                            aaCCTCTTTTTTCATAAATTTTAAGTAACCACGGATGCAATCTTGCAGATGTACCTAAAGTAACTGCCGCTGACTTTAACGTATCTCGCAAAAATGCTTCTTCAACATAAGTAAGTAATTGGCTACCATAGCCTTTCCCtt                  <  1:108108/141‑1 (MQ=255)
                                                                                                                               ctctTTTTTCATAAATTTTAAGTAACCACGGATGCAATCTTGCAGATGTACCTAAAGTAACTGCCGCTGACTTTAACGTATCTCGCAAAAATGCTTCTTCAACATAAGTAAGTAATTGGCTACCATAGCCTTTCCCTTCAt               >  1:136442/1‑141 (MQ=255)
                                                                                                                                   tttttCATAAATTTTAAGTAACCACGGATGCAATCTTGCAGATGTACCTAAAGTATCTGCCGCTGACTTTAACGTATCTCGCAAAAATGCTTCTTCAACATAAGTAAGTAATTGGCTACCATAGCCTTTCCCTTCATACTc           <  3:160616/141‑1 (MQ=255)
                                                                                                                                        cATAAATTTTAAGTAACCACGGATGCAATCTTGCAGATGTACCTAAAGTAACTGCCGCTGACTTTAACGTATCTCGCAAAAATGCTTCTTCAACATAAGTAAGTAATTGGCTACCATAGCCTTTCCCTTCATACTCAGGAt      >  3:328655/1‑141 (MQ=255)
                                                                                                                                         aTAAATTTTAAGTAACCACGGATGCAATCTTGCAGATGTACCTAAAGTAACTGCCGCTGACTTTAACGTATCTCGCAAAAATGCTTCTTCAACATAAGTAAGTAATTGGCTACCATAGCCTTTCCCTTCATACTCAGGAtt     >  3:456769/1‑141 (MQ=255)
                                                                                                                                          tAAATTTTAAGTAACCACGGATGCAATCTTGCAGATGTACCTAAAGTAACTGCCGCTGACTTTAACGTATCTCGCAAAAATGCTTCTTCAACATAAGTAAGTAATTGGCTACCATAGCCTTTCCCTTCATACTCAGGAttt    <  3:257849/141‑1 (MQ=255)
                                                                                                                                           aaaTTTTAAGTAACCACCGATGCAATCTTGCAGATGCACCTAAAGTAACTGCCGCTGACTTTAACGTATCTCGCAAAAATGCTTCTTCAACATAAGTAAGTAATTGGCTACCATAGCCTTTCCCTTCATACTCAGGATTTg   <  1:105652/141‑1 (MQ=255)
                                                                                                                                            aaTTTTAAGTAACCACGGATGCAATCTTGCAGATGTACCTAAAGTAACTGCCGCTGACTTTAACGTATCTCGCAAAAATGCTTCTTCAACATAAGTAAGTAATTGGCTACCATAGCCTTTCCCTTCATACTCAGGATTTGt  <  2:660377/141‑1 (MQ=255)
                                                                                                                                            aaTTTTAAGTAACCACGGATGCAATCTTGCAGATGTACCTAAAGTAACTGCCGCTGACTTTAACGTATCTCGCAAAAATGCTTCTTCAACATAAGTAAGTAATTGGCTACCATAGCCTTTCCCTTCATACTCAGGATTTGt  >  2:352176/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                            
ATATTATTATCTTAAAATGGTGGTTGGCCTAATATTGTTTCGTCAAAGCGCTCGGGTATCAATACTTTGCGCATGATCACACCTAAATCGCCATCATCATTTTCATGTTCGCTGTATATTTCATAACCTCTTTTTTCATAAATTTTAAGTAACCACGGATGCAATCTTGCAGATGTACCTAAAGTAACTGCCGCTGACTTTAACGTATCTCGCAAAAATGCTTCTTCAACATAAGTAAGTAATTGGCTACCATAGCCTTTCCCTTCATACTCAGGATTTGT  >  USA300TCH1516_ALE/2525647‑2525927

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: