BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	2,528,073	A→G	5.5%	S43S (AGT→AGC)	USA300TCH1516_02412 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,528,073	0	A	G	5.5%	447.1 / 11.5	147	S43S (AGT→AGC)	USA300TCH1516_02412	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (69/69); new base G (6/2); total (76/71)
Fisher's exact test for biased strand distribution p-value = 2.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.73e-01

Only 100 of 147 total aligned reads displayed.

CAAATGAGTCAATATATTCAATTAAATATGGACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAATCGTCATCTTCCCACTCC  >  USA300TCH1516_ALE/2527934‑2528212

cAAATGAGTCAATATATTCAATTAAATATGGACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTAc                                                                                                                                            <  1:433694/141‑1 (MQ=255)

 aaaTGAGTCAATATATTCAATTAAATATGGACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACt                                                                                                                                           <  1:54533/141‑1 (MQ=255)

  aaTGGGTCAATATGTTCAGTTAAATATGGACGCTTAGCCGTTTTATATTCTGCTAATGTATTGGTGGTCATTGTAGGTGTTTTGATCGCATAAGAGGCATCACCGCCTGAATCTGCATTTAGGCGAAACGCTTTGGTACTa                                                                                                                                          <  4:86822/141‑1 (MQ=255)

  aaTGAGTCAATATATTCAATTAAATATGGACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTa                                                                                                                                          <  2:6485/141‑1 (MQ=255)

  aaTGAGTCAATATATTCAATTAAATATGGACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTa                                                                                                                                          <  3:345280/141‑1 (MQ=255)

   aTGAGTCAATATATTCAATTAAATATGGACGCTTAACCGTTTTATATTCTGCTAATGTATGGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAg                                                                                                                                         >  4:222132/1‑141 (MQ=255)

    tGAGTCAATATATTCAATTAAATATGGACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGc                                                                                                                                        <  2:613584/141‑1 (MQ=255)

         cAATATATTCAATTAAATATGGACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCtt                                                                                                                                   >  1:207450/1‑141 (MQ=255)

          aaTATATTCAATTAAATATGGACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCttt                                                                                                                                  >  2:1086189/1‑141 (MQ=255)

             atatTCAATTAAATATGGACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTgg                                                                                                                               <  1:308261/141‑1 (MQ=255)

               atTCAATTAAATATGGACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGAt                                                                                                                             >  1:131892/1‑141 (MQ=255)

                ttCAATTAAATATGGACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATg                                                                                                                            >  4:35722/1‑141 (MQ=255)

                   aaTTAAATATGGACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGaaa                                                                                                                         >  1:240807/1‑141 (MQ=255)

                    aTTAAATATGGACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAAc                                                                                                                        >  2:80078/1‑141 (MQ=255)

                    aTTAAATATGGACGCTTAACCGTTTTAGATTCTGCTGATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATACAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACAATTTGATTCTAGCGTCTTTTGGATGAAAc                                                                                                                        >  2:675068/1‑141 (MQ=255)

                        aaTATGGACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACAAtt                                                                                                                    >  3:9055/1‑141 (MQ=255)

                         atatGGACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCAttt                                                                                                                   >  1:994010/1‑141 (MQ=255)

                            tGGACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTc                                                                                                                >  1:892974/1‑141 (MQ=255)

                              gACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCaa                                                                                                              >  4:233041/1‑141 (MQ=255)

                              gACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCaa                                                                                                              <  1:280796/141‑1 (MQ=255)

                                  cTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAaataa                                                                                                          >  2:1084764/1‑141 (MQ=255)

                                     aaCCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGc                                                                                                       <  1:421141/141‑1 (MQ=255)

                                     aaCCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTGTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGc                                                                                                       <  3:289578/141‑1 (MQ=255)

                                      aCCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATCATGCt                                                                                                      >  1:868445/1‑141 (MQ=255)

                                      aCCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCt                                                                                                      <  3:384468/141‑1 (MQ=255)

                                         gTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTNGATGAAACCATTNTTCAAATAATGCtttt                                                                                                   <  1:730903/141‑1 (MQ=255)

                                           tttATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGt                                                                                                 <  1:225998/141‑1 (MQ=255)

                                            ttATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTc                                                                                                >  3:376598/1‑141 (MQ=255)

                                            ttATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTc                                                                                                >  3:413061/1‑141 (MQ=255)

                                            ttATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTc                                                                                                >  4:135381/1‑141 (MQ=255)

                                             tataTTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCg                                                                                               <  3:191351/141‑1 (MQ=255)

                                              atatTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGt                                                                                              <  4:17865/141‑1 (MQ=255)

                                                 ttCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACCCTTTGGTACTAGCGTCTTTAGGATGAAACCATTTATCAAATAATGCTTTTGTCGtata                                                                                           <  3:178073/141‑1 (MQ=255)

                                                 ttCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGtata                                                                                           >  2:507368/1‑141 (MQ=255)

                                                   cTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGCCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAgg                                                                                         >  2:590696/1‑141 (MQ=255)

                                                      cTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCAt                                                                                      <  4:412415/141‑1 (MQ=255)

                                                        aaTGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCa                                                                                    <  1:794541/141‑1 (MQ=255)

                                                        aaTGTATAGCTGGTCATTGTAGGTGTTGTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCa                                                                                    <  2:150976/141‑1 (MQ=255)

                                                         aTGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCaa                                                                                   >  1:366712/1‑141 (MQ=255)

                                                           gTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAac                                                                                 >  1:525142/1‑141 (MQ=255)

                                                              taGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACAcc                                                                              <  1:1023512/141‑1 (MQ=255)

                                                              taGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACAcc                                                                              <  1:205697/141‑1 (MQ=255)

                                                                 cTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCttt                                                                           >  1:660753/1‑141 (MQ=255)

                                                                 cTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCttt                                                                           <  3:148496/141‑1 (MQ=255)

                                                                  tGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAATACTTTTGTGCTAGCCCCTTTTGGATGAAACCATTTTTCAAATAATTCTTTTGTCGTATAGGCATCAAACACCtttt                                                                          <  3:182998/141‑1 (MQ=255)

                                                                       aTTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATc                                                                     <  3:27675/141‑1 (MQ=255)

                                                                        ttGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAATACTTTTGTGCTAGCCCCTTTTGGATGAAACCATTTTTCAAATAATTCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCg                                                                    >  2:724492/1‑141 (MQ=255)

                                                                        ttGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCg                                                                    >  3:450378/1‑141 (MQ=255)

                                                                          gTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGt                                                                  >  3:130352/1‑141 (MQ=255)

                                                                           tAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTg                                                                 >  1:353108/1‑141 (MQ=255)

                                                                            aGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGACTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGc                                                                >  3:360970/1‑141 (MQ=255)

                                                                             ggTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGCAACCATTTTTCCAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCt                                                               <  2:226852/141‑1 (MQ=255)

                                                                              gtgtTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCtt                                                              >  2:617892/1‑141 (MQ=255)

                                                                                gtTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCt                                                            <  1:470191/141‑1 (MQ=255)

                                                                                 ttttAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCTTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTa                                                           >  2:462877/1‑141 (MQ=255)

                                                                                    tAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAATACTTTTGTGCTAGCCCCTTTTGGATGAAACCATTTTTCAAATAATTCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCg                                                        >  1:271390/1‑141 (MQ=255)

                                                                                    tAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAATCCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCg                                                        <  1:715895/141‑1 (MQ=255)

                                                                                    tAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCg                                                        <  2:190226/141‑1 (MQ=255)

                                                                                    tAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCg                                                        <  1:993803/141‑1 (MQ=255)

                                                                                     aaTCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTTTTTTTTTTTTTTTGTCGTGTAGGCATCAAACATTTTTTTAATCGGTGTTTCTTTCGt                                                       >  2:434163/1‑141 (MQ=255)

                                                                                     aaTCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGt                                                       >  2:46564/1‑141 (MQ=255)

                                                                                       tCGCATAAAATGCATCACCGCCTGGAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTc                                                     <  1:897939/141‑1 (MQ=255)

                                                                                       acGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTc                                                     <  1:908253/140‑1 (MQ=255)

                                                                                        cGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCt                                                    >  1:389594/1‑141 (MQ=255)

                                                                                         gCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCTGTGCTTCTCtctttctt                                                   >  4:285549/1‑141 (MQ=255)

                                                                                          cATAAAATGCATCACCGCCTGAAACAGCATTAACGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCATTCTTg                                                  >  2:582142/1‑141 (MQ=255)

                                                                                           aTAAAATGCATCACCGCCTGAAACAGCATTAAAGCGACACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGa                                                 >  1:786891/1‑141 (MQ=255)

                                                                                           aTAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGa                                                 >  4:182347/1‑141 (MQ=255)

                                                                                            tAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGaa                                                >  4:244566/1‑141 (MQ=255)

                                                                                                aTGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGaaaaaa                                            >  2:929869/1‑141 (MQ=255)

                                                                                                  gCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATTCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAaata                                          >  4:264661/1‑141 (MQ=255)

                                                                                                     tCACCGCCTGAAACAGCATTAAAGCGAAATACTTTTGTGCTAGCCCCTTTTGGATGAAACCATTTTTCAAATAATTCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAAtt                                       >  1:1020457/1‑141 (MQ=255)

                                                                                                     tCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAAtt                                       <  3:135799/141‑1 (MQ=255)

                                                                                                      cACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAAttt                                      <  3:296216/141‑1 (MQ=255)

                                                                                                      cACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAAttt                                      >  4:419341/1‑141 (MQ=255)

                                                                                                       aCCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAAtttt                                     <  3:88914/141‑1 (MQ=255)

                                                                                                        ccGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTtta                                    <  1:907320/141‑1 (MQ=255)

                                                                                                         ctccTGAAACAGCATTAAATCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTttat                                   <  2:1024663/139‑1 (MQ=255)

                                                                                                              gAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCgg                                                                   <  4:191142/104‑1 (MQ=255)

                                                                                                              gAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCt                              >  2:49160/1‑141 (MQ=255)

                                                                                                                aaCAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTc                            <  2:726621/141‑1 (MQ=255)

                                                                                                                aaCAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTc                            >  4:79316/1‑141 (MQ=255)

                                                                                                                aaCAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTc                            >  1:336439/1‑141 (MQ=255)

                                                                                                                 aCAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTAAAAAAAAAAAAAAAATAATTTTATTATCTTCa                           <  2:341694/141‑1 (MQ=255)

                                                                                                                      aTTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTTTTTTTTTTTTTTTCACATATAGGCATCAAACACCTTTTCAATCGGTTTTTCTATCGATCTTGTAATCATACTTTTATCATCTACAACtat                      >  2:1045045/1‑139 (MQ=255)

                                                                                                                      aTTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTTTTTTTTTTTCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACttt                      >  2:350338/1‑141 (MQ=255)

                                                                                                                         aaaGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAAt                   <  1:1061227/141‑1 (MQ=255)

                                                                                                                         aaaGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAAt                   >  1:364319/1‑141 (MQ=255)

                                                                                                                          aaGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAATc                  <  3:285443/141‑1 (MQ=255)

                                                                                                                          aaGCGAAACACTTTGGTACTAGAGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTAAAGATCATGAAAAAATAATTTTATTATCTTCAACTTTAATc                  <  4:18536/141‑1 (MQ=255)

                                                                                                                           aGCGAAATACTTTTGTGCTAGCCCCTTTTGGATGAAACCATTTTTCAAATAATTCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAATCg                 >  1:463584/1‑141 (MQ=255)

                                                                                                                            gCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAATCGt                >  2:196261/1‑141 (MQ=255)

                                                                                                                              gAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAATCGTCa              >  1:253099/1‑141 (MQ=255)

                                                                                                                               aaaCACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAATCGTCAt             >  3:325493/1‑141 (MQ=255)

                                                                                                                                aaCACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAATCGTCATc            <  2:626878/141‑1 (MQ=255)

                                                                                                                                aaCACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTATAAAAAAAAAAAAAAAATAATTTTATTATCTTCAACTTTAATCGTCATc            <  2:239270/141‑1 (MQ=255)

                                                                                                                                 acacTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAATCGTCATCt           <  1:630575/141‑1 (MQ=255)

                                                                                                                                     tttGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAATCGTCATCTTccc       <  1:116505/141‑1 (MQ=255)

                                                                                                                                     tttGGTACTAGCGTCTTTTGGATGAAACCATTTCTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAATCGTCATCTTccc       <  2:126337/141‑1 (MQ=255)

                                                                                                                                          tACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGTTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAATCGTCATCTTCCCACTcc  >  2:690297/1‑141 (MQ=255)

CAAATGAGTCAATATATTCAATTAAATATGGACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAATCGTCATCTTCCCACTCC  >  USA300TCH1516_ALE/2527934‑2528212

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`