BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	2,528,079	G→C	6.3%	D41E (GAC→GAG) ‡	USA300TCH1516_02412 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,528,079	0	G	C	6.3%	477.0 / 18.8	142	D41E (GAC→GAG) ‡	USA300TCH1516_02412	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (70/63); new base C (6/3); total (76/66)
Fisher's exact test for biased strand distribution p-value = 5.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

Only 100 of 143 total aligned reads displayed.

AGTCAATATATTCAATTAAATATGGACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAATCGTCATCTTCCCACTCCCTATCTT  >  USA300TCH1516_ALE/2527940‑2528219

aGTCAATATATTCAATTAAATATGGACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGt                                                                                                                                             >  2:156051/1‑141 (MQ=255)

  tCAATATATTCAATTAAATATGGACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCt                                                                                                                                           >  1:547513/1‑141 (MQ=255)

   cAATATATTCAATTAAATATGGACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCtt                                                                                                                                          >  1:207450/1‑141 (MQ=255)

    aaTATATTCAATTAAATATGGACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCttt                                                                                                                                         >  2:1086189/1‑141 (MQ=255)

    aaTATATTCAATTAAATATGGACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCACATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCttt                                                                                                                                         <  1:455462/141‑1 (MQ=255)

       atatTCAATTAAATATGGACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTgg                                                                                                                                      <  1:308261/141‑1 (MQ=255)

        tatTCAATTAAATATGGACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGa                                                                                                                                     <  4:278666/141‑1 (MQ=255)

         atTCAATTAAATATGGACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGAt                                                                                                                                    >  1:131892/1‑141 (MQ=255)

             aaTTAAATATGGACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGaaa                                                                                                                                >  1:240807/1‑141 (MQ=255)

              aTTAAATATGGACGCTTAACCGTTTTAGATTCTGCTGATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATACAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACAATTTGATTCTAGCGTCTTTTGGATGAAAc                                                                                                                               >  2:675068/1‑141 (MQ=255)

               ttAAATATGGACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAATACTTTTGTGCTAGCCCCTTTTGGATGAAAcc                                                                                                                              >  2:93560/1‑141 (MQ=255)

                  aaTATGGACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACAAtt                                                                                                                           >  3:9055/1‑141 (MQ=255)

                   atatGGACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCAttt                                                                                                                          >  1:994010/1‑141 (MQ=255)

                     atGGACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCAttttt                                                                                                                        <  3:290683/141‑1 (MQ=255)

                        gACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCaa                                                                                                                     >  4:233041/1‑141 (MQ=255)

                             ttAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAaataat                                                                                                                <  1:688836/141‑1 (MQ=255)

                               aaCCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTGTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGc                                                                                                              <  3:289578/141‑1 (MQ=255)

                                aCCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATCATGCt                                                                                                             >  1:868445/1‑141 (MQ=255)

                                aCCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCt                                                                                                             <  3:384468/141‑1 (MQ=255)

                                  cGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCttt                                                                                                           >  1:272911/1‑141 (MQ=255)

                                   tttttATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCtttt                                                                                                          <  2:610239/140‑1 (MQ=255)

                                   gTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTNGATGAAACCATTNTTCAAATAATGCtttt                                                                                                          <  1:730903/141‑1 (MQ=255)

                                   gTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCtttt                                                                                                          <  1:786207/141‑1 (MQ=255)

                                     tttATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGt                                                                                                        <  1:225998/141‑1 (MQ=255)

                                      ttATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTc                                                                                                       >  4:135381/1‑141 (MQ=255)

                                      ttATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTc                                                                                                       >  3:413061/1‑141 (MQ=255)

                                      ttATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTc                                                                                                       >  3:376598/1‑141 (MQ=255)

                                       tataTTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCg                                                                                                      <  4:238406/141‑1 (MQ=255)

                                        atatTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGt                                                                                                     <  4:17865/141‑1 (MQ=255)

                                           ttCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACCCTTTGGTACTAGCGTCTTTAGGATGAAACCATTTATCAAATAATGCTTTTGTCGtata                                                                                                  <  3:178073/141‑1 (MQ=255)

                                           ttCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGtata                                                                                                  >  2:507368/1‑141 (MQ=255)

                                             cTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGCCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAgg                                                                                                >  2:590696/1‑141 (MQ=255)

                                                cTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCAt                                                                                             <  4:412415/141‑1 (MQ=255)

                                                  aaTGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCa                                                                                           <  1:794541/141‑1 (MQ=255)

                                                  aaTGTATAGCTGGTCATTGTAGGTGTTGTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCa                                                                                           <  2:150976/141‑1 (MQ=255)

                                                     gTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAac                                                                                        >  1:525142/1‑141 (MQ=255)

                                                       ataGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAacac                                                                                      <  1:482107/141‑1 (MQ=255)

                                                        taGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACAcc                                                                                     <  1:205697/141‑1 (MQ=255)

                                                        taGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACAcc                                                                                     <  1:1023512/141‑1 (MQ=255)

                                                           cTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCttt                                                                                  <  3:148496/141‑1 (MQ=255)

                                                           cTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCttt                                                                                  >  1:660753/1‑141 (MQ=255)

                                                            tGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAATACTTTTGTGCTAGCCCCTTTTGGATGAAACCATTTTTCAAATAATTCTTTTGTCGTATAGGCATCAAACACCtttt                                                                                 <  3:182998/141‑1 (MQ=255)

                                                                 aTTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATc                                                                            <  3:27675/141‑1 (MQ=255)

                                                                  ttGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAATACTTTTGTGCTAGCCCCTTTTGGATGAAACCATTTTTCAAATAATTCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCg                                                                           >  2:724492/1‑141 (MQ=255)

                                                                  ttGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCg                                                                           >  3:450378/1‑141 (MQ=255)

                                                                     tAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCACATAATGCTTTTGTCGTATAGGCATCAAACACCTCTTCAATCGGTg                                                                        >  2:757046/1‑141 (MQ=255)

                                                                     tAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTg                                                                        >  1:353108/1‑141 (MQ=255)

                                                                     tAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTg                                                                        >  4:440999/1‑141 (MQ=255)

                                                                     tAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATAAATCACCTTTTCAATCGGTg                                                                        >  1:826644/1‑141 (MQ=255)

                                                                      aGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGACTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGc                                                                       >  3:360970/1‑141 (MQ=255)

                                                                       ggTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGCAACCATTTTTCCAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCt                                                                      <  2:226852/141‑1 (MQ=255)

                                                                           ttttAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCTTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTa                                                                  >  2:462877/1‑141 (MQ=255)

                                                                           ttttAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTa                                                                  <  3:278886/141‑1 (MQ=255)

                                                                              tAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAATACTTTTGTGCTAGCCCCTTTTGGATGAAACCATTTTTCAAATAATTCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCg                                                               >  1:271390/1‑141 (MQ=255)

                                                                              tAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCg                                                               <  1:249786/141‑1 (MQ=255)

                                                                              tAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCg                                                               <  2:190226/141‑1 (MQ=255)

                                                                              tAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCg                                                               <  1:993803/141‑1 (MQ=255)

                                                                               aaTCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGt                                                              >  2:46564/1‑141 (MQ=255)

                                                                                 tCGCATAAAATGCATCACCGCCTGGAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTc                                                            <  1:897939/141‑1 (MQ=255)

                                                                                 tCGCATAAAATGCATCACCGCCTGGAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTc                                                            <  1:897861/141‑1 (MQ=255)

                                                                                 acGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTc                                                            <  1:908253/140‑1 (MQ=255)

                                                                                  cGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCt                                                           >  1:389594/1‑141 (MQ=255)

                                                                                   gCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCTGTGCTTCTCtctttctt                                                          >  4:285549/1‑141 (MQ=255)

                                                                                    cATAAAATGCATCACCGCCTGAAACAGCATTAACGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCATTCTTg                                                         >  2:582142/1‑141 (MQ=255)

                                                                                     aTAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGa                                                        >  4:182347/1‑141 (MQ=255)

                                                                                      tAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGaa                                                       >  4:244566/1‑141 (MQ=255)

                                                                                          aTGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGaaaaaa                                                   >  2:929869/1‑141 (MQ=255)

                                                                                          aTGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGaaaaaa                                                   >  4:397966/1‑141 (MQ=255)

                                                                                          aTGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGaaaaaa                                                   >  1:358462/1‑141 (MQ=255)

                                                                                            gCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAaata                                                 >  1:393203/1‑141 (MQ=255)

                                                                                               tCACCGCCTGAAACAGCATTAAAGCGAAATACTTTTGTGCTAGCCCCTTTTGGATGAAACCATTTTTCAAATAATTCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAAtt                                              >  1:1020457/1‑141 (MQ=255)

                                                                                               tCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAAtt                                              <  3:135799/141‑1 (MQ=255)

                                                                                                 aCCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAAtttt                                            <  3:88914/141‑1 (MQ=255)

                                                                                                 aCCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTATTCGAAAAATAAtttt                                            >  1:332266/1‑141 (MQ=255)

                                                                                                  ccGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTtta                                           <  1:907320/141‑1 (MQ=255)

                                                                                                        gAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCgg                                                                          >  3:191142/1‑104 (MQ=255)

                                                                                                        gAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCgg                                                                          <  4:191142/104‑1 (MQ=255)

                                                                                                        gAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCt                                     >  2:49160/1‑141 (MQ=255)

                                                                                                          aaCAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTc                                   >  4:79316/1‑141 (MQ=255)

                                                                                                          aaCAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTc                                   <  2:726621/141‑1 (MQ=255)

                                                                                                          aaCAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTc                                   >  2:429856/1‑141 (MQ=255)

                                                                                                           aCAGCATTAAAGCGAAATACTTTTGTGCTAGCCCCTTTTGGATGAAACCATTTTTCAAATAATTCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCa                                  <  2:1045680/141‑1 (MQ=255)

                                                                                                           aCAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCa                                  >  2:623621/1‑141 (MQ=255)

                                                                                                           aCAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTAAAAAAAAAAAAAAAATAATTTTATTATCTTCa                                  <  2:341694/141‑1 (MQ=255)

                                                                                                             aGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAAc                                >  3:336365/1‑141 (MQ=255)

                                                                                                                aTTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTTTTTTTTTTTTTTTCACATATAGGCATCAAACACCTTTTCAATCGGTTTTTCTATCGATCTTGTAATCATACTTTTATCATCTACAACtat                             >  2:1045045/1‑139 (MQ=255)

                                                                                                                aTTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTTTTTTTTTTTCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACttt                             >  2:350338/1‑141 (MQ=255)

                                                                                                                   aaaGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAAt                          <  1:1061227/141‑1 (MQ=255)

                                                                                                                   aaaGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAAt                          >  1:364319/1‑141 (MQ=255)

                                                                                                                    aaacGACACACTTTTGTACTACCCTCTTTTTGATTAAACCATTATTCAATTAATGCTTTTGTCTTATTTTCCTCAAACACCTTTTCAATCGCTACTTCTACAGATCAAAAAAAAATAATTTTATTATCTTCAACTTTAATc                         <  2:1015214/138‑1 (MQ=255)

                                                                                                                    aaGCGAAACACTTTGGTACTAGAGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTAAAGATCATGAAAAAATAATTTTATTATCTTCAACTTTAATc                         <  4:18536/141‑1 (MQ=255)

                                                                                                                     aGCGAAATACTTTTGTGCTAGCCCCTTTTGGATGAAACCATTTTTCAAATAATTCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAATCg                        >  1:463584/1‑141 (MQ=255)

                                                                                                                      gCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAATCGt                       >  2:196261/1‑141 (MQ=255)

                                                                                                                         aaaCACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAATCGTCAt                    >  3:325493/1‑141 (MQ=255)

                                                                                                                          aaCACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAATCGTCATc                   <  2:626878/141‑1 (MQ=255)

                                                                                                                          aaCACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTATAAAAAAAAAAAAAAAATAATTTTATTATCTTCAACTTTAATCGTCATc                   <  2:239270/141‑1 (MQ=255)

                                                                                                                              cTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTTTATTATTGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATTGGTGCTTCTATCGTTCTTGAAACAATAATTTTATTATCTTCAACTTTAATCGTCCTCTTcc               >  2:11893/1‑141 (MQ=255)

                                                                                                                               tttGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAATCGTCATCTTccc              <  1:116505/141‑1 (MQ=255)

                                                                                                                                    tACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGTTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAATCGTCATCTTCCCACTcc         >  2:690297/1‑141 (MQ=255)

                                                                                                                                          cGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAATCGTCATCTTCCCACTCCCTATCt   <  2:161844/141‑1 (MQ=255)

AGTCAATATATTCAATTAAATATGGACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAATCGTCATCTTCCCACTCCCTATCTT  >  USA300TCH1516_ALE/2527940‑2528219

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`