BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	2,528,080	T→C	6.4%	D41G (GAC→GGC) ‡	USA300TCH1516_02412 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,528,080	0	T	C	6.4%	407.2 / 14.0	142	D41G (GAC→GGC) ‡	USA300TCH1516_02412	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (67/65); new base C (7/2); total (75/67)
Fisher's exact test for biased strand distribution p-value = 1.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.46e-01

Only 100 of 144 total aligned reads displayed.

AGTCAATATATTCAATTAAATATGGACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAATCGTCATCTTCCCACTCCCTATCTTT  >  USA300TCH1516_ALE/2527940‑2528220

aGTCAATATATTCAATTAAATATGGACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGt                                                                                                                                              >  2:156051/1‑141 (MQ=255)

   cAATATATTCAATTAAATATGGACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCtt                                                                                                                                           >  1:207450/1‑141 (MQ=255)

    aaTATATTCAATTAAATATGGACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCttt                                                                                                                                          >  2:1086189/1‑141 (MQ=255)

    aaTATATTCAATTAAATATGGACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCACATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCttt                                                                                                                                          <  1:455462/141‑1 (MQ=255)

       atatTCAATTAAATATGGACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTgg                                                                                                                                       <  1:308261/141‑1 (MQ=255)

        tatTCAATTAAATATGGACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGa                                                                                                                                      <  4:278666/141‑1 (MQ=255)

         atTCAATTAAATATGGACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGAt                                                                                                                                     >  1:131892/1‑141 (MQ=255)

          ttCAATTAAATATGGACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATg                                                                                                                                    <  3:384524/141‑1 (MQ=255)

           tCAATTAAATATGGACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAATACTTTTGTGCTAGCCCCTTTTGGATGa                                                                                                                                   >  4:182998/1‑141 (MQ=255)

             aaTTAAATATGGACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGaaa                                                                                                                                 >  1:240807/1‑141 (MQ=255)

              aTTAAATATGGACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAAc                                                                                                                                >  2:80078/1‑141 (MQ=255)

              aTTAAATATGGACGCTTAACCGTTTTAGATTCTGCTGATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATACAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACAATTTGATTCTAGCGTCTTTTGGATGAAAc                                                                                                                                >  2:675068/1‑141 (MQ=255)

               ttAAATATGGACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAATACTTTTGTGCTAGCCCCTTTTGGATGAAAcc                                                                                                                               >  2:93560/1‑141 (MQ=255)

                   atatGGACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCAttt                                                                                                                           >  1:994010/1‑141 (MQ=255)

                     atGGACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCAttttt                                                                                                                         <  3:290683/141‑1 (MQ=255)

                      tGGACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTc                                                                                                                        >  1:892974/1‑141 (MQ=255)

                            cTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAaataa                                                                                                                  >  2:1084764/1‑141 (MQ=255)

                                aCCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCt                                                                                                              <  3:384468/141‑1 (MQ=255)

                                  cGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCttt                                                                                                            >  1:272911/1‑141 (MQ=255)

                                   tttttATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCtttt                                                                                                           <  2:610239/140‑1 (MQ=255)

                                   gTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTNGATGAAACCATTNTTCAAATAATGCtttt                                                                                                           <  1:730903/141‑1 (MQ=255)

                                   gTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCtttt                                                                                                           <  1:786207/141‑1 (MQ=255)

                                     tttATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGt                                                                                                         <  1:225998/141‑1 (MQ=255)

                                      ttATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTc                                                                                                        >  4:135381/1‑141 (MQ=255)

                                       tataTTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCg                                                                                                       <  4:238406/141‑1 (MQ=255)

                                       tataTTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCg                                                                                                       <  3:191351/141‑1 (MQ=255)

                                           ttCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACCCTTTGGTACTAGCGTCTTTAGGATGAAACCATTTATCAAATAATGCTTTTGTCGtata                                                                                                   <  3:178073/141‑1 (MQ=255)

                                                cTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCAt                                                                                              <  4:412415/141‑1 (MQ=255)

                                                  aaTGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCa                                                                                            <  1:794541/141‑1 (MQ=255)

                                                  aaTGTATAGCTGGTCATTGTAGGTGTTGTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCa                                                                                            <  2:150976/141‑1 (MQ=255)

                                                   aTGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTCGCGTCTTTTGGATGAACCCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCaa                                                                                           <  4:146341/141‑1 (MQ=255)

                                                   aTGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCaa                                                                                           >  2:209327/1‑141 (MQ=255)

                                                   aTGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCaa                                                                                           >  1:366712/1‑141 (MQ=255)

                                                     gTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAac                                                                                         >  1:525142/1‑141 (MQ=255)

                                                       ataGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAacac                                                                                       <  1:482107/141‑1 (MQ=255)

                                                        taGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACAcc                                                                                      <  1:205697/141‑1 (MQ=255)

                                                        taGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACAcc                                                                                      <  1:1023512/141‑1 (MQ=255)

                                                           cTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCttt                                                                                   >  1:660753/1‑141 (MQ=255)

                                                                  ttGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAATACTTTTGTGCTAGCCCCTTTTGGATGAAACCATTTTTCAAATAATTCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCg                                                                            >  2:724492/1‑141 (MQ=255)

                                                                  ttGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCg                                                                            >  3:450378/1‑141 (MQ=255)

                                                                    gTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGt                                                                          >  3:130352/1‑141 (MQ=255)

                                                                     tAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCACATAATGCTTTTGTCGTATAGGCATCAAACACCTCTTCAATCGGTg                                                                         >  2:757046/1‑141 (MQ=255)

                                                                     tAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTg                                                                         >  1:353108/1‑141 (MQ=255)

                                                                     tAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTg                                                                         >  4:440999/1‑141 (MQ=255)

                                                                     tAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATAAATCACCTTTTCAATCGGTg                                                                         >  1:826644/1‑141 (MQ=255)

                                                                      aGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGACTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGc                                                                        >  3:360970/1‑141 (MQ=255)

                                                                       ggTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGCAACCATTTTTCCAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCt                                                                       <  2:226852/141‑1 (MQ=255)

                                                                       ggTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCt                                                                       <  2:488853/141‑1 (MQ=255)

                                                                        gtgtTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCtt                                                                      >  2:617892/1‑141 (MQ=255)

                                                                           ttttAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCTTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTa                                                                   >  2:462877/1‑141 (MQ=255)

                                                                           ttttAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTa                                                                   <  3:278886/141‑1 (MQ=255)

                                                                              tAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAATACTTTTGTGCTAGCCCCTTTTGGATGAAACCATTTTTCAAATAATTCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCg                                                                >  1:271390/1‑141 (MQ=255)

                                                                              tAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAATCCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCg                                                                <  1:715895/141‑1 (MQ=255)

                                                                              tAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCg                                                                <  1:249786/141‑1 (MQ=255)

                                                                              tAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCg                                                                <  1:993803/141‑1 (MQ=255)

                                                                              tAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCg                                                                <  2:190226/141‑1 (MQ=255)

                                                                               aaTCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTTTTTTTTTTTTTTTGTCGTGTAGGCATCAAACATTTTTTTAATCGGTGTTTCTTTCGt                                                               >  2:434163/1‑141 (MQ=255)

                                                                                 tCGCATAAAATGCATCACCGCCTGGAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTc                                                             <  1:897861/141‑1 (MQ=255)

                                                                                 acGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTc                                                             <  1:908253/140‑1 (MQ=255)

                                                                                  cGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCt                                                            >  1:389594/1‑141 (MQ=255)

                                                                                   gCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCTGTGCTTCTCtctttctt                                                           >  4:285549/1‑141 (MQ=255)

                                                                                    cATAAAATGCATCACCGCCTGAAACAGCATTAACGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCATTCTTg                                                          >  2:582142/1‑141 (MQ=255)

                                                                                     aTAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGa                                                         >  4:182347/1‑141 (MQ=255)

                                                                                      tAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGaa                                                        >  4:244566/1‑141 (MQ=255)

                                                                                       aaaaTGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGaaa                                                       <  3:171301/141‑1 (MQ=255)

                                                                                         aaTGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGaaaaa                                                     <  2:717632/141‑1 (MQ=255)

                                                                                          aTGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGaaaaaa                                                    >  4:397966/1‑141 (MQ=255)

                                                                                          aTGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGaaaaaa                                                    >  1:358462/1‑141 (MQ=255)

                                                                                            gCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAaata                                                  >  1:393203/1‑141 (MQ=255)

                                                                                               tCACCGCCTGAAACAGCATTAAAGCGAAATACTTTTGTGCTAGCCCCTTTTGGATGAAACCATTTTTCAAATAATTCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAAtt                                               >  1:1020457/1‑141 (MQ=255)

                                                                                               tCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAAtt                                               <  3:135799/141‑1 (MQ=255)

                                                                                                cACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAAttt                                              <  3:296216/141‑1 (MQ=255)

                                                                                                 aCCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAAtttt                                             <  3:88914/141‑1 (MQ=255)

                                                                                                 aCCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTATTCGAAAAATAAtttt                                             >  1:332266/1‑141 (MQ=255)

                                                                                                  ccGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTtta                                            <  1:907320/141‑1 (MQ=255)

                                                                                                   ctccTGAAACAGCATTAAATCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTttat                                           <  2:1024663/139‑1 (MQ=255)

                                                                                                     ccTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACATTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTttatta                                         >  4:194991/1‑141 (MQ=255)

                                                                                                        gAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATATTGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCt                                      >  2:651658/1‑141 (MQ=255)

                                                                                                        gAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCgg                                                                           >  3:191142/1‑104 (MQ=255)

                                                                                                        gAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCgg                                                                           <  4:191142/104‑1 (MQ=255)

                                                                                                        gAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCt                                      >  2:49160/1‑141 (MQ=255)

                                                                                                        gAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCt                                      >  3:37384/1‑141 (MQ=255)

                                                                                                          aaCAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTc                                    >  4:79316/1‑141 (MQ=255)

                                                                                                          aaCAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTc                                    >  2:429856/1‑141 (MQ=255)

                                                                                                           aCAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCa                                   >  2:623621/1‑141 (MQ=255)

                                                                                                           aCAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTAAAAAAAAAAAAAAAATAATTTTATTATCTTCa                                   <  2:341694/141‑1 (MQ=255)

                                                                                                                aTTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTTTTTTTTTTTTTTTCACATATAGGCATCAAACACCTTTTCAATCGGTTTTTCTATCGATCTTGTAATCATACTTTTATCATCTACAACtat                              >  2:1045045/1‑139 (MQ=255)

                                                                                                                   aaaGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAAt                           <  1:1061227/141‑1 (MQ=255)

                                                                                                                    aaGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAATc                          <  3:285443/141‑1 (MQ=255)

                                                                                                                     aGCGAAATACTTTTGTGCTAGCCCCTTTTGGATGAAACCATTTTTCAAATAATTCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAATCg                         >  1:463584/1‑141 (MQ=255)

                                                                                                                         aaaCACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAATCGTCAt                     >  3:325493/1‑141 (MQ=255)

                                                                                                                           acacTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAATCGTCATCt                   <  1:630575/141‑1 (MQ=255)

                                                                                                                             tcTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCTACTTTAATCGTCATCTTc                 <  2:34019/140‑1 (MQ=255)

                                                                                                                               tttGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAATCGTCATCTTccc               <  1:116505/141‑1 (MQ=255)

                                                                                                                               tttGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAATCGTCATCTTccc               <  3:74472/141‑1 (MQ=255)

                                                                                                                               tttGGTACTAGCGTCTTTTGGATGAAACCATTTCTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAATCGTCATCTTccc               <  2:126337/141‑1 (MQ=255)

                                                                                                                                  ggTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAATCGTCATCTTCCCACt            <  2:547513/141‑1 (MQ=255)

                                                                                                                                      cTAGCGTCTTTTGGATGAAACCATTTTTTTTTTTTTTCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAATCGTCATCTTCCCACTCCCt        >  2:177541/1‑141 (MQ=255)

                                                                                                                                           gTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAATCGTCATCTTCCCACTCCCTATCtt   <  1:906527/141‑1 (MQ=255)

                                                                                                                                            tCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAATCGTCATCTTCCCACTCCCTATCttt  >  4:261998/1‑141 (MQ=255)

AGTCAATATATTCAATTAAATATGGACGCTTAACCGTTTTATATTCTGCTAATGTATAGCTGGTCATTGTAGGTGTTTTAATCGCATAAAATGCATCACCGCCTGAAACAGCATTAAAGCGAAACACTTTGGTACTAGCGTCTTTTGGATGAAACCATTTTTCAAATAATGCTTTTGTCGTATAGGCATCAAACACCTTTTCAATCGGTGCTTCTATCGTTCTTGAAAAAATAATTTTATTATCTTCAACTTTAATCGTCATCTTCCCACTCCCTATCTTT  >  USA300TCH1516_ALE/2527940‑2528220

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`