Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 2,530,786 A→G 5.2% intergenic (‑37/+236) yefM ← / ← bdbD Antitoxin YefM/Disulfide bond formation protein D

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,530,7860AG 5.2% 362.8 / 6.9 120intergenic (‑37/+236)yefM/bdbDAntitoxin YefM/Disulfide bond formation protein D
Reads supporting (aligned to +/- strand):  ref base A (69/41);  new base G (3/3);  total (74/46)
Fisher's exact test for biased strand distribution p-value = 6.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

Only 100 of 127 total aligned reads displayed.

TTATCATCAGTAGATGTTACAGTTACCATAACACTATCATCATTTACTTTTGTCATAAGTGCCTTTAAATTCTGTCGAGCGTATGAATAATTTTTAATAATCATATTTATCGCCTCCTTTTAACACACCTCAATTGTACAAATATCTGTACAATAAAACAAGCGACACAGAAAAAAACTACTAATCAAGATTTCGCACGGGGAAGAATCAATACCTATCCTGTACTGATTCCCTACTAAACAATGTTTACATGATAATTTCCACCTATTTAGACTTCATAC  >  USA300TCH1516_ALE/2530646‑2530926
                                                                                                                                            |                                                                                                                                            
 tATCATCAGTAGATGTTACAGTTACCATAACACTATCATCATTTACTTTTGTCATAAGTGCCTTTAAATTCTGTCGAGCGTATGAATAATTTTTAATAATCATATTTATCGCCTCCTTTTAACACACCTCAATTGTACaaa                                                                                                                                             <  3:81208/141‑1 (MQ=255)
  atcatcAGTAGATGTTACAGTTACCATCACACTATCATCATTTACTTTTGTCATAAGTGCCTTTAAATTCTGTCGAGCGTATGAATAATTTTTAATAATCATATTTATCGCCTCCTTTTAACACACCTCAATTGTACAAat                                                                                                                                            >  1:68408/1‑141 (MQ=255)
  atcatcAGTAGATGTTACAGTTACCATAACACTATCATCATTTACTTTTGTCATAAGTGCCTTTAAATTCTGTCGAGCGTATGAATAATTTTTAATAATCATATTTATCGCCTCCTTTTAACACACCTCAATTGTACAAat                                                                                                                                            >  1:793148/1‑141 (MQ=255)
  atcatcAGTAGATGTTACAGTTACCATAACACTATCATCATTTACTTTTGTCATAAGTGCCTTTAAATTCTGTCGAGCGTATGAATAATTTTTAATAATCATATTTATCGCCTCCTTTTAACACACCTCAATTGTACAAat                                                                                                                                            >  3:295511/1‑141 (MQ=255)
  atcatcAGTAGATGTTACAGTTACCATAACACTATCATCATTTACTTTTGTCATAAGTGCCTTTAAATTCTGTCGAGCGTATGAATAATTTTTAATAATCATATTTATCGCCTCCTTTTAACACACCTCAATTGTACAAat                                                                                                                                            >  1:402121/1‑141 (MQ=255)
  atcatcAGTAGATGTTACAGTTACCATAACACTATCATCATTTACTTTTGTCATAAGTGCCTTTAAATTCTCTCCAGCGTATGAATAATTTTTAATAATCATATTTATCGCCTCCTTTTAACACACCTCAATTGTACAAat                                                                                                                                            >  3:340115/1‑141 (MQ=255)
      tcaGTAGATGTTACAGTTACCATAACACTATCATCATTTACTTTTGTCATAAGTGCCTTTAAATTCTGTCGAGCGTATGAATAATTTTTAATAATCATATTTATTGCCTCCTTTTAACACACCTCAATTGTACAGatattt                                                                                                                                        <  4:53032/141‑3 (MQ=255)
       caGTAGATGTTACAGTTACCATAACACTATCATCATTTACTTTTGTCATAAGTGCCTTTAAATTCTGTCGAGCGTATGAATAATTTTTAATAATCATATTTATCGCCTCCTTTTAACACACCTCAATTGTACAGatatttg                                                                                                                                       <  2:326145/141‑4 (MQ=255)
            gATGTTACAGTTACCATAACACTATCATCATTTACTTTTGTCATAAGTGCCTTTAAATTCTGTCGAGCGTATGAATAATTTTTAATAATCATATTTATCGCCTCCTTTTAACACACCTCAATTGTACAAATATCTGTACaa                                                                                                                                  <  1:835272/141‑1 (MQ=255)
             aTGTTACAGTTACCATAACACTATCATCATTTACTTTTGTCATAAGTGCCTTTAAATTCTGTCGAGCGTATGAATAATTTTTAATAATCATATTTATCGCCTCCTTTTAACACACCTCAATTGTACAAATATCTGTACAAt                                                                                                                                 <  3:415949/141‑1 (MQ=255)
               gTTACAGTTACCATAACACTATCATCATTTACTTTTGTCATAAGTGCCTTTAAATTCTGTCGAGCGTATGAATAATTTTTAATAATCATATTTATCGCCTCCTTTTAACACACCTCAATTGTACACATATCTGTACAATaa                                                                                                                               >  2:327469/1‑141 (MQ=255)
                ttACAGTTACCATAACACTATCATCATTTACTTTTGTCATAAGTGCCTTTAAATTCTGTCGAGCGTATGAATAATTTTTAATAATCATATTTATCGCCTCCTTTTAACACACCTCAATTGTACAAATATCTGTACAATaaa                                                                                                                              >  2:559250/1‑141 (MQ=255)
                ttACAGTTACCATAACACTATCATCATTTACTTTTGTCATAAGTGCCTTTAAATTCTGTCGAGCGTATGAATAATTTTTAATAATCATATTTATCGCCTCCTTTTAACACACCTCAATTGTACAAATATCTGTACAATaaa                                                                                                                              <  4:396208/141‑1 (MQ=255)
                      ttACCATAACACTATCATCATTTACTTTTGTCATAAGTGCCTTTAAATTCTGTCGAGCGTATGAATAATTTTTAATAATCATATTTATCGCCTCCTTTTAACACACCTCAATTGTACAAATATCTGTACAATAAAACAAGc                                                                                                                        >  2:274356/1‑141 (MQ=255)
                      ttACCATAACACTATCATCATTTACTTTTGTCATAAGTGCCTTTAAATTCTGTCGAGCGTATGAATAATTTTTAATAATCATATTTATCGCCTCCTTTTAACACACCTCAATTGTACAAATATCTGTACAATAAAACAAGc                                                                                                                        >  2:817489/1‑141 (MQ=255)
                         ccATAACACTATCATCATTTACTTTTGTCATAAGTGCCTTTAAATTCTGTCGAGCGTATGAATAATTTTTAATAATCATATTTATCGCCTCCTTTTAACACACCTCAATTGTACAAATATCTGTACAATAAAACAAGCGac                                                                                                                     <  1:778523/141‑1 (MQ=255)
                         ccATAACACTATCATCATTTACTTTTGTCATAAGTGCCTTTAAATTCTGTCGAGCGTATGAATAATTTTTAATAATCATATTTATCGCCTCCTTTTAACACACCTCAATTGTACAAATATCTGTACAATAAAACAAGCGac                                                                                                                     >  1:773758/1‑141 (MQ=255)
                         ccATAACACTATCATCATTTACTTTTGTCATAAGTGCCTTTAAATTCTGTCGAGCGTATGAATAATTTTTAATAATCATATTTATCGCCTCCTTTTAACACACCTCAATTGTACAAATATCTGTACAATAAAACAAGCGac                                                                                                                     >  1:704056/1‑141 (MQ=255)
                           aTAACACTATCATCATTTACTTTTGTCATAAGTGCCTTTAAATTCTGTCGAGCGTATGAATAATTTTTAATAATCATATTTATCGCCTCCTTTTAACACACCTCAATTGTACAAATATCTGTACAATAAAACAAGCGacac                                                                                                                   >  3:246140/1‑141 (MQ=255)
                           aTAACACTATCATCATTTACTTTTGTCATAAGTGCCTTTAAATTCTGTCGAGCGTATGAATAATTTTTAATAATCATATTTATCGCCTCCTTTTAACACACCTCAATTGTACAAATATCTGTACAATAAAACAAGCGacac                                                                                                                   <  2:775580/141‑1 (MQ=255)
                           aTAACACTATCATCATTTACTTTTGTCATAAGTGCCTTTAAATTCTGTCGAGCGAAAGAATAATTTTTAATAATCATATTTCTCGCCTCCTTTTAACACACCTCAATTGTACATATATCTGTTCAATAAAACaaaaaaaaa                                                                                                                   <  2:312823/141‑8 (MQ=255)
                               cacTATCATCATTTACTTTTGTCATAAGTGCCTTTAAATTCTGTCGAGCGTATGAATAATTTTTAATAATCATATTTATCGCCTCCTTTTAACACACCTCAATTGTACAAATATCTGTACAATAAAACAAGCGACACAGaa                                                                                                               <  1:585903/141‑1 (MQ=255)
                                  tATCATCATTTACTTTTGTCATAAGTGCCTTTAAATTCTGTCGAGCGTATGAATAATTTTTAATAATCATATTTATCGCCTCCTTTTAACACACCTCAATTGTACAAATATCTGTACAATAAAACAAGCGACACAGaaaaa                                                                                                            >  1:586363/1‑141 (MQ=255)
                                    tcatcaTTTACTTTTGTCATAAGTGCCTTTAAATTCTGTCGAGCGTATGAATAATTTTTAATAATCATATTTATCGCCTCCTTTTAACACACCTCAATTGTACAAATATCTGTACAATAAAACAAGCGACACAGaaaaaaa                                                                                                          <  1:867595/141‑1 (MQ=255)
                                     catcatTTACTTTTGTCATAAGTGCCTTTAAATTCTGTCGAGCGTATGAATAATTTTTAATAATCATATTTATCGCCTCCTTTTAACACACCTCAATTGTACAAATATCTGTACAATAAAACAAGCGACACAGAAAAAAac                                                                                                         >  1:138835/1‑141 (MQ=255)
                                           ttACTTTTGTCATAAGTGCCTTTAAATTCTGTCGAGCGTATGAATAATTTTTTTTTTTTTTTTTTATCGCATCCTGTTAACACACCTTAATTGTACAAATATCTGTACAATAAAACAATCGTCACAGTCAAAAACTATTaa                                                                                                   >  4:257434/1‑141 (MQ=255)
                                           ttACTTTTGTCATAAGTGCCTTTAAATTCTGTCGAGCGTATGAATAATTTTTAATAATCATATTTATCGCCTCCTTTTAACACACCTCAATTGTACAAATATCTGTACAATAAAACAAGCGACACAGAAAAAAACTACTaa                                                                                                   >  1:134820/1‑141 (MQ=255)
                                                   gTCATAAGTGCCTTTAAATTCTGTCGAGCGTATGAATAATTTTTAATTTTTATATTTATCGCCTCCTTTTAACACACCTCAATTGTACAAATATCTGTACAATAAAACAAGCGACACAGAAAAAAACTACTAATCAAGAtt                                                                                           >  2:625420/1‑141 (MQ=255)
                                                    tCATAAGTGCCTTTAAATTCTGTCGAGCGTATGAATAATTTTTAATAATCATATTTATCGCCTCCTTTTAACACACCTCAATTGTACAAATATCTGTACAATAAAACAAGCGACACAGAAAAAAACTACTAATCAAGAttt                                                                                          >  3:381181/1‑141 (MQ=255)
                                                        aaGTGCCTTTAAATTCTGTCGAGCGTATGAATAATTTTTAATAATCATATTTATCGCCTCCTTTTAACACACCTCAATTGTACAAATATCTGTACAATAAAACAAGCGACACAGAAAAAAGCTACTAATCAAGATTTCGCa                                                                                      >  4:142376/1‑141 (MQ=255)
                                                            gCCTTTAAATTCTGTCGAGCGTATGAATAATTTTTAATAATCATATTTATCGCCTCCTTTTAACACACCTCAATTGTACAAATATCTGTACAATAAAACAAGCGACACAGAAAAAAACTACTAATCAAGATTTCGCACggg                                                                                  >  1:475661/1‑141 (MQ=255)
                                                             ccTTTAAATTCTGTCGAGCGTATGAATAATTTTTAATAATCATATTTATCGCCTCCTTTTAACACACCTCAATTGTACAAATATCTGTACAATAAAACAAGCGACACAGAAAAAAACTACTAATCAAGATTTCGCACgggg                                                                                 >  2:378387/1‑141 (MQ=255)
                                                             ccTTTAAATTCTGTCGAGCGTATGAATAATTTTTAATAATCATATTTATCGCCTCCTTTTAACACACCTCAATTGTACAAATATCTGTACAATAAAACAAGCGACACAGAAAAAAACTACTAATCAAGATTTCGCACgggg                                                                                 <  1:766015/141‑1 (MQ=255)
                                                             ccTTTAAATTCTGTCGAGCGTATGAATAATTTTTAATAATCATATTTATCGCCTCCTTTTAACACACCTCAATTGTACAAATATCTGTACAATAAAACAAGCGACACAGAAAAAAACTACTAATCAAGATTTCGCACgggg                                                                                 >  1:529762/1‑141 (MQ=255)
                                                              cTTTAAATTCTGTCGAGCGTATGAATAATTTTTAATAATCATATTTATCGCCTCCTTTTAACACACCTCAATTGTACAAATATCTGTACAATAAAACACGCGACACAGAAAAAAACTACTAATCAAGATTTCGCACGGGga                                                                                <  1:158668/141‑1 (MQ=255)
                                                              cTTTAAATTCTGTCGAGCGTATGAATAATTTTTAATAATCATATTTATCGCCTCCTTTTAACACACCTCAATTGTACAAATATCTGTACAATAAAACAAGCGACACAGAAAAAAACTACTAATCAAGATTTCGCACGGGga                                                                                <  3:403855/141‑1 (MQ=255)
                                                               tttAAATTCTGTCGAGCGTATGAATAATTTTTAATAATCATATTTATCGCCTCCTTTTAACACACCTCAATTGTACAAATATCTGTACAATAAAACAAGCGACACAGAAAAAAACTACTAATCAAGATTTCGCACGGGgaa                                                                               <  1:885332/141‑1 (MQ=255)
                                                               tttAAATTCTGTCGAGCGTATGAATAATTTTTAATAATCATATTTATCGCCTCCTTTTAACACACCTCAATTGTACAAATATCTGTACAATAAAACAAGCGACACAGAAAAAAACTACTAATCAAGATTTCGCACGGGgaa                                                                               >  2:625535/1‑141 (MQ=255)
                                                                ttAAATTCTGTCGAGCTTATGAATAATTTTTAATACTCAGATTTATCGCCTCCTTTTAACACACCTCAATTGTACATATACCTGTACAATAAATCAAGCGACACAGAAAAAAACTACTAATCAAGTTTTCTCACCGGgaag                                                                              <  2:635895/141‑1 (MQ=255)
                                                                   aaTTCTGTCGAGAGTATGAATAATTTTTAATAATCATATTTATCGCCTCCTTTTAACACACCTCAATTGTACAAATATCTGTACAATAAAACAAGCGACACAGAAAAAAACTACTAATCAAGATTTCGCAACGGGAAGAAt                                                                           >  2:997453/1‑141 (MQ=255)
                                                                     ttCTGTCGAGCGTATGAATAATTTTTAATAATCATATTTATCGCCTCCTTTTAACACACCTCAATTGTACAAATATCTGTACAATAAAACAAGCGACACAGAAAAAAACTACTAATCAAGATTTCGCACGGGGAAGAATCa                                                                         <  1:441383/141‑1 (MQ=255)
                                                                     ttCTGTCGAGCGTATGAATAATTTTTAATAATCATATTTATCGCCTCCTTTTAACACACCTCAATTGTACAAATATCTGTACAATAAAACAAGCGACACAGAAAAAAACTACTAATCAAGATTTCGCACGGGGAAGAATCa                                                                         <  1:351267/141‑1 (MQ=255)
                                                                      tCTGTCGAGCGTATGAATAATTTTTAATAATCATATTTATCGCCTCCTTTTAACACACCTCAATTGTACAGATATTTGTAc                                                                                                                                    >  4:13039/1‑81 (MQ=255)
                                                                       cTGTCGAGCGTATGAATAATTTTTAATAATCATATTTATCGCCTCCTTTTAACACACCTCAATTGTACAAATATCTGTACAATAAAACAAGCGACACAGAAAAAAACTACTAATCAAGATTTCGCACGGGGAAGAATCAAt                                                                       >  3:291758/1‑141 (MQ=255)
                                                                       cTGTCGAGCGTATGAATAATTTTTAATAATCATATTTATCGCCTCCTTTTAACACACCTCAATTGTACAAATATCTGTACAATAAAACAAGCGACACAGAAAAAAACTACTAATCAAGATTTCGCACGGGGAAGAATCAAt                                                                       >  2:245378/1‑141 (MQ=255)
                                                                         gTCGAGCGTATGAATAATTTTTAATAATCATATTTATCGCCTCCTTTTAACACACCTCAATTGTACAAATATCTGTACAATAAAACAAGCGACACAGAAAAAAACTACTAATCAAGATTTCGCACGGGGAAGAATCAATAc                                                                     >  4:451415/1‑141 (MQ=255)
                                                                         gTCGAGCGTATGAATAATTTTTAATAATCATATTTATCGCCTCCTTTTAACACACCTCAATTGTACAAATATCTGTACAATAAAACAAGCGACACAGAAAAAAACTACTAATCAAGATTTCGCACGGGGAAGAATCAATAc                                                                     <  1:1016548/141‑1 (MQ=255)
                                                                              gCGTATGAATAATTTTTAATAATCATATTTATCGCCTCCTTTTAACACACCTCAATTGTACAAATATCTGTACAATAAAACAAGCGACACAGAAAAAAACTACTAATCAAGATTTCGCACGGGGAAGAATCAATACCTATc                                                                <  2:979299/141‑1 (MQ=255)
                                                                               cGTATGAATAATTTTTAATAATCATATTTATCGCCTCCTTTTAACACACCTCAATTGTACAAATATCTGTACAATAAAACAAGCGACACAGAAAAAAACTACTAATCAAGATTTCGCACGGTGAAGAATCAATACCTATcc                                                               <  1:571362/141‑1 (MQ=255)
                                                                                 tATGAATAATTTTTAATAATCATATTTATCGCCTCCTTTTAACACACCTCAATTGTACAAATATCTGTACAATAAAACAAGCGACACAGAAAAAAACTACTAATCAAGATTTCGCACGGGGAAGAATCAATACCTATCCTg                                                             >  4:264514/1‑141 (MQ=255)
                                                                                  aTGAATAATTTTTAATAATCATATTTATCGCCTCCTTTTAACACACCTCAATTGTACAAATATCTGTACAATAAAACAAGCGACACAGAAAAAAACTACTAATCAAGATTTCGCACGGGGAAGAATCAATACCTATCCTGt                                                            <  2:328172/141‑1 (MQ=255)
                                                                                     aataatTTTTAATAATCATATTTATCGCCTCCTTTTAACACACCTCAATTGTACAAATATCTGTACAATAAAACAAGCGACACAGAAAAAAACTACTAATCAAGATTTCGCACGGGGAAGAATCAATACCTATCCTGTACt                                                         <  1:396176/141‑1 (MQ=255)
                                                                                      ataatTTTTAATAATCATATTTATCGCCTCCTTTTAACACACCTCAATTGTACAAATATCTGTACAATAAAACAAGCGACACAGAAAAAAACTACTAATCAAGATTTCGCACGGGGAAGAATCAATACCTATCCTGTACTg                                                        <  2:104823/141‑1 (MQ=255)
                                                                                      ataatTTTTAATAATCATATTTATCGCCTCCTTTTAACACACCTCAATTGTACAAATATCTGTACAATAAAACAAGCGACACAGAAAAAAACTACTAATCAAGATTTCGCACGGGGAAGAATCAATACCTATCCTGTACTg                                                        >  1:629183/1‑141 (MQ=255)
                                                                                          tttttAATAATCATATTTATCGCCTCCTTTTAACACACCTCAATTGTACAAATATCTGTACAATAAAACAAGCGACACAGAAAAAAACTACTAATCAAGATTTCGCACGGGGAAGAATCAATACCTATCCTGTACTGATTc                                                    <  2:1059556/141‑1 (MQ=255)
                                                                                           ttttAATAATCATATTTATCGCCTCCTTTTAACACACCTCAATTGTACAAATATCTGTACAATAAAACAAGCGACACAGAAAAAAACTACTAATCAAGATTTCGCACGGGGAAGAATCAATACCTATCCTGTACTGATTcc                                                   >  4:129396/1‑141 (MQ=255)
                                                                                            tttAATAATCATATTTATCGCCTCCTTTTAACACACCTCAATTGTACAAATATCTGTACAATAAAACAAGCGACACAGAAAAAAACTACTAATCAAGATTTCGCACGGGGAAGAATCAATACCTATCCTGTACTGATTccc                                                  >  1:277233/1‑141 (MQ=255)
                                                                                                ataatCATATTTATCGCCTCCTTTTAACACACCTCAATTGTACAAATATCTGTACAATAAAACAAGCGACACAGAAAAAAACTACTAATCAAGATTTCGCACGGGGAAGAATCAATACCTATCCTGTACTGATTCCctact                                              >  2:834355/1‑141 (MQ=255)
                                                                                                    tCATATTTATCGCCTCCTTTTAACACACCTCAATTGTACAAATATCTGTACAATAAAACAAGCGACACAGAAAAAAACTACTAATCAAGATTTCGCACGGGGAAGAATCAATACCTATCCTGTACTGATTCCCTACTAAAc                                          <  1:1086002/141‑1 (MQ=255)
                                                                                                    tCATATTTATCGCCTCCTTTTAACACACCTCAATTGTACAAATATCTGTACAATAAAACAAGCGACACAGAAAAAAACTACTAATCAAGATTTCGCACGGGGAAGAATCAATACCTATCCTGTACTGATTCCCTACTAAAc                                          >  2:1046520/1‑141 (MQ=255)
                                                                                                      atatTTATCGCCTCCTTTTAACACGCCTCAATTGTACAAATATCTGTACAATAAAACAAGCGACACAGAAAAAAACTACTAATCAAGATTTCGCACGGGGAAGAATCAATACCTATCCTGTACTGATTCCCTACTAAACaa                                        <  1:1047138/141‑1 (MQ=255)
                                                                                                        atTTATCGCCTCCTTTTAACACACCTCAATTGTACAAATATCTGTACAATAAAACAAGCGACACAGAAAAAAACTACTAATCAAGATTTCGCACGGGGAAGAATCAATACCTATCCTGTACTGATTCCCTACTAAACAATg                                      >  2:765855/1‑141 (MQ=255)
                                                                                                           tATCGCCTCCTTTTAACACACCTCAATTGTACAAATATCTGTACAATAAAACAAGCGACACAGAAAAAAACTACTAATCAAGATTTCGCACGGGGAAGAATCAATACCTATCCTGTACTGATTCCCTACTAAACAATGttt                                   >  4:93585/1‑141 (MQ=255)
                                                                                                           tATCGCCTCCTTTTAACACACCTCAATTGTACAAATATCTGTACAATAAAACAAGCGACACAGAAAAAAACTACTAATCAAGATTTCGCACGGGGAAGAATCAATACCTATCCTGTACTGATTCCCTACTAAACAATGttt                                   >  2:114627/1‑141 (MQ=255)
                                                                                                            aTCGCCTCCTTTTAACACACCTCAATTGTACAAATATCTGTACAATAAAACAAGCGACACAGAAAAAAACTACTAATCAAGATTTCGCACGGGGAAGAATCAATACCTATCCTGTACTGATTCCCTACTAAACAATGTTTa                                  >  2:1005825/1‑141 (MQ=255)
                                                                                                             tCGCCTCCTTTTAACACACCTCAATTGTACAAATATCTGTACAATAAAACAAGCGACACAGAAAAAAACTACTAATCAAGATTTCGCACGGGGAAGAATCAATACCTATCCTGTACTGATTCCCTACTAAACAATGTTTAc                                 >  3:441689/1‑141 (MQ=255)
                                                                                                                cctcctTTTAACACACCTCAATTGTACAAATATCTGTACAATAAAACAAGCGACACAGAAAAAAACTACTAATCAAGATTTCGCACGGGGAAGAATCAATACCTATCCTGTACTGATTCCCTACTAAACAATGTTTACATg                              <  3:451415/141‑1 (MQ=255)
                                                                                                                cctcctTTTAACACACCTCAATTGTACAAATATCTGTACAATAAAACAAGCGACACAGAAAAAAACTACTAATCAAGATTTCGCACGGGGAAGAATCAATACCTATCCTGTACTGATTCCCTACTAAACAATGTTTACATg                              <  3:379371/141‑1 (MQ=255)
                                                                                                                   cctTTTAACACACCTCAATTGTACAAATATCTGTACAATAAAACAAGCGACACAGAAAAAAACTACTAATCAAGATTTCGCACGGGGAAGAATCAATACCTATCCTGTACTGATTCCCTACTAAACAATGTTTACATGATa                           >  2:50845/1‑141 (MQ=255)
                                                                                                                     ttttAACACACCTCAATTGTACAAATATCTGTACAATAAAACAAGCGACACAGAAAAAAACTACTAATCAAGATTTCGCACGGGGAAGAATCAATACCTATCCTGTACTGATTCCCTACTAAACAATGTTTACATGATAAt                         >  2:950462/1‑141 (MQ=255)
                                                                                                                      tttAACACACCTCAATTGTACAAATATCTGTACAATAAAACAAGCGACACAGAAAAAAACTACTAATCAAGATTTCGCACGGGGAAGAATCAATACCTATCCTGTACTGATTCCCTACTAAACAATGTTTACATGATAAtt                        <  1:463195/141‑1 (MQ=255)
                                                                                                                       ttAACACACCTCAATTGTACAAATATCTGTACAATAAAACAAGCGACACAGAAAAAAACTACTAATCAAGATTTCGCACGGGGAAGAATCAATACCTATCCTGTACTGATTCCCTACTAAACAATGTTTACATGATAAttt                       <  2:842931/141‑1 (MQ=255)
                                                                                                                       ttAACACACCTCAATTGTACAAATATCTGTACAATAAAACAAGCGACACAGAAAAAAACTACTAATCAAGATTTCGCACGGGGAAGAATCAATACCTATCCTGTACTGATTCCCTACTAAACAATGTTTACATGATAAttt                       <  1:60759/141‑1 (MQ=255)
                                                                                                                       ttAACACACCTCAATTGTACAAATATCTGTACAATAAAACAAGCGACACAGAAAAAAACTACTAATCAAGATTTCGCACGGGGAAGAATCAATACCTATCCTGTACTGATTCCCTACTAAACAATGTTTACATGATAAttt                       <  3:156419/141‑1 (MQ=255)
                                                                                                                         aaCACACCTCAATTGTACAAATATCTGTACAATAAAACAAGCGACACAGAAAAAAACTACTAATCAAGATTTCGCACGGGGAAGAATCAATACCTATCCTGTACTGATTCCCTACTAAACAATGTTTACATGATAATTTcc                     <  2:852126/141‑1 (MQ=255)
                                                                                                                         aaCACACCTCAATTGTACAAATATCTGTACAATAAAACAAGCGACACAGAAAAAAACTACTAATCAAGATTTCGCACGGGGAAGAATCAATACCTATCCTGTACTGATTCCCTACTAAACAATGTTTACATGATAATTTcc                     <  4:345021/141‑1 (MQ=255)
                                                                                                                         aaCACACCTCAATTGTACAAATATCTGTACAATAAAACAAGCGACACAGAAAAAAACTACTAATCAAGATTTCGCACGGGGAAGAATCAATACCTATCATGTACTGATTAAATAATAAACAATGTTTACATGATAATTTcc                     >  1:48176/1‑141 (MQ=255)
                                                                                                                          acacacCTCAATTGTACAAATATCTGTACAATAAAACAAGCGACACAGAAAAAAACTACTAATCAAGATTTCGCACGGGGAAGAATCAATACCTATCCTGTACTGATTCCCTACTAAACAATGTTTACATGATAATTTCCa                    >  1:990966/1‑141 (MQ=255)
                                                                                                                           cacacCTCAATTGTACAAATATCTGTACAATAAAACAAGCGACACAGAAAAAAACTACTAATCAAGATTTCGCACGGGGAAGAATCAATACCTATCCTGTACTGATTCCCTACTAAACAATGTTTACATGATAATTTCCAc                   >  1:345509/1‑141 (MQ=255)
                                                                                                                            acacCTCAATTGTACAAATATCTGTACAATAAAACAAGCGACACAGAAAAAAACTACTAATCAAGATTTCGCACGGGGAAGAATCAATACCTATCCTGTACTGATTCCCTACTAAACAATGTTTACATGATAATTTCCAcc                  <  1:565776/141‑1 (MQ=255)
                                                                                                                             cacCTCAATTGTACAAATATCTGTACAATAAAACAAGCGACACAGAAAAAAACTCCTAATCAAGATTTCGCACGGGGAAGAAACAATACCTATCCTGTACTGATTCCCTACTAAACAATGTTTACATGATAATTTCCACCt                 >  4:343021/1‑141 (MQ=255)
                                                                                                                              acCTCAATTGTACAAATATCTGTACAATAAAACAAGCGACACAGAAAAAAACTACTAATCAAGATTTCGCACGGGGAAGAATCAATACCTATCCTGTACTGATTCCCTACTAAACAATGTTTACATGATAATTTCCACCTa                >  2:947346/1‑141 (MQ=255)
                                                                                                                              acCTCAATTGTACAAATATCTGTACAATAAAACAAGCGACACAGAAAAAAACTACTAATCAAGATTTCGCACGGGGAAGAATCAATACCTATCCTGTACTGATTCCCTACTAAACAATGTTTACATGATAATTTCCACCTa                <  1:894700/141‑1 (MQ=255)
                                                                                                                                cTCAATTGTACAAATATCTGTACAATAAAACAAGCGACACAGAAAAAAACTACTAATCAAGATTTCGCACGGGGAAGAATCAATACCTATCCTGTACTGATTCCCTACTAAACAATGTTTACATGATAATTTCCACCTAtt              <  4:251380/141‑1 (MQ=255)
                                                                                                                                   aaTTGTACAAATATCTGTACAATAAAACAAGCGACACAGAAAAAAACTACTAATCAAGATTTCGCACGGGGAAGAATCAATACCTATCCTGTACTGATTCCCTACTAAACAATGTTTACATGATAATTTCCACCTATTTAg           <  1:559250/141‑1 (MQ=255)
                                                                                                                                    aTTGTACAAATATCTGTACAATAAAACAAGCGACACAGAAAAAAACTACTAATCAAGATTTCGCACGGGGAAGAATCAATACCTATCCTGTACTGATTCCCTACTAAACAATGTTTACATGATAATTTCCACCTATTTAGa          >  2:614884/1‑141 (MQ=255)
                                                                                                                                     ttGTACAAATATCTGTACAATAAAACAAGCGACACAGAAAAAAACTACTAATCAAGATTTCGCACGGGGAAGAATCAATACCTATCCTGTACTGATTCCCTACTAAACAATGTTTACATGATAATTTCCACCTATTTAGAc         >  1:35597/1‑141 (MQ=255)
                                                                                                                                     ttGTACAAATATCTGTACAATAAAACAAGCGACACAGAAAAAAACTACTAATCAAGATTTCGCACGGGGAAGAATCAATACCTATCCTGTACTGATTCCCTACTAAACAATGTTTACATGATAATTTCCACCTATTTAGAc         >  4:245594/1‑141 (MQ=255)
                                                                                                                                      tGTACAGATATTTGTACAATAAAACAAGCGACACAGAAAAAAACTACTAATCAAGATTTCGCACGGGGAAGAATAAATACCTATCCTGTCCTGATTACCTACTAAACAATGTTTACATGATAATTTCCACCTATTTAGACt        >  1:217882/1‑141 (MQ=255)
                                                                                                                                       gTACAAATATCTGTACAATAAAACAAGCGACACAGAAAAAAACTACTAATCAAGATTTCGCACGGGGAAGAATCAATACCTATCCTGTACTGATTCCCTACTAAACAATGTTTACATGATAATTTCTACCTATTTAGACtt       >  1:295245/1‑141 (MQ=255)
                                                                                                                                       gTACAAATATCTGTACAATAAAACAAGCGACACAGAAAAAAACTACTAATCAAGATTTCGCACGGGGAAGAATCAATACCTATCCTGTACTGATTCCCTACTAAACAATGTTTACATGATAATTTCCACCTATTTAGACtt       >  2:907850/1‑141 (MQ=255)
                                                                                                                                       gTACAAATATCTGTACAATAAAACAAGCGACACAGAAAAAAACTACTAATCAAGATTTCGCACGGGGAAGAATCAATACCTATCCTGTACTGATTCCCTACTAAACAATGTTTACATGATAATTTCCACCTATTTAGACtt       >  3:180682/1‑141 (MQ=255)
                                                                                                                                        tACAGATATTTGTACAATAAAACAAGCGACACAGAAAAAAACTACTAATCAAGATTTCGCACGGGGAAGAATCAATACCTATCCTGTACTGATTCCCTACTAAACAATGTTTACATGATAATTTCTACCTATTTAGACTTc      >  2:580916/1‑141 (MQ=255)
                                                                                                                                         aCAAATATCTGTACAATAAAACAAGCGACACAGAAAAAAACTACTAATCAAGATTTCGCACGGGGAAGAATCAATACCTATCCTGTACTGATTCCCTACTAAACAATGTTTACATGATAATTTCCACCTATTTAGACTTCa     >  2:586203/1‑141 (MQ=255)
                                                                                                                                         aCAAATATCTGTACAATAAAACAAGCGACACAGAAAAAAACTACTAATCAAGATTTCGCACGGGGAAGAATCAATACCTATCCTGTACTGATTCCCTACTAAACAATGTTTACATGATAATTTCCACCTATTTAGACTTCa     >  3:439015/1‑141 (MQ=255)
                                                                                                                                          cAAATATCTGTACAATAAAACAAGCGACACAGAAAAAAACTACTAATCAAGATTTCGCACGGGGAAGAATCAATACCTATCCTGTACTGATTCCCTACTAAACAATGTTTACATGATAATTTCCACCTATTTAGACTTCAt    <  1:995048/141‑1 (MQ=255)
                                                                                                                                           aaaTATCTGTACAATAAAACAAAAAAAAAAAAAAAAAACTACTAATCAAGATTTCGCACGGGGAAGAATCAATACCTATCCTGTACTGATTCCCTACTAAACAATGTTTACATGATAATTTCCACCTATTTAGACTTCATa   <  4:402325/141‑1 (MQ=255)
                                                                                                                                            aaTATCTGTACAATAAAACAAGCGACACAGAAAAAAGCTACTAATCAAGATTTCGCACGGGGAAGAATCAATACCTATCCTGTACTGATTCCCTACTAAACAATGTTTACATGATAATTTCCACCTATTTAGACTTCATAc  <  3:142376/141‑1 (MQ=255)
                                                                                                                                            aaTATCTGTACAATAAAACAAGCGACACAGAAAAAAACTACTAATCAAGATTTCGCACGGGGAAGAATCAATACCTATCCTGTACTGATTCCCTACTAAACAATGTTTACATGATAATTTcc                     <  2:1012588/122‑1 (MQ=255)
                                                                                                                                            aaTATCTGTACAATAAAACAAGCGACACAGAAAAAAACTACTAATCAAGATTTCGCACGGGGAAGAATCAATACCTATCCTGTACTGATTCCCTACTAAACAATGTTTACATGATAATTTcc                     >  1:1012588/1‑122 (MQ=255)
                                                                                                                                            |                                                                                                                                            
TTATCATCAGTAGATGTTACAGTTACCATAACACTATCATCATTTACTTTTGTCATAAGTGCCTTTAAATTCTGTCGAGCGTATGAATAATTTTTAATAATCATATTTATCGCCTCCTTTTAACACACCTCAATTGTACAAATATCTGTACAATAAAACAAGCGACACAGAAAAAAACTACTAATCAAGATTTCGCACGGGGAAGAATCAATACCTATCCTGTACTGATTCCCTACTAAACAATGTTTACATGATAATTTCCACCTATTTAGACTTCATAC  >  USA300TCH1516_ALE/2530646‑2530926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: