Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 2,559,032 T→A 6.3% V146V (GTT→GTA bcr_2 → Bicyclomycin resistance protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,559,0320TA 6.3% 452.8 / 14.3 143V146V (GTT→GTAbcr_2Bicyclomycin resistance protein
Reads supporting (aligned to +/- strand):  ref base T (61/73);  new base A (4/5);  total (65/78)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.87e-01

Only 100 of 146 total aligned reads displayed.

GATCAATTGCCATTATTCTTAACTTTACGATTTATTCAAGGTTTAACTGGTGGTGGCGTCATCGTGATTGCAAAAGCCTCTGCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCATCATTTTAATTGGCGTCGCTTCTCAATTACCTAAAACA  >  USA300TCH1516_ALE/2558892‑2559170
                                                                                                                                            |                                                                                                                                          
  tCAATTGCCATTATTCTTAACTTTACGATTTATTCAAGGTTTAACTGGTGGTGGCGTCATCGTGATTGCAAAAGCCTCTGCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTaa                                                                                                                                          >  3:426033/1‑141 (MQ=255)
    aaTTGCCATTATTCTTAACTTTACGATTTATTCAAGGTTTAACTGGTGGTGGCGTCATCGTGATTGCAAAAGCCTCTGCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATg                                                                                                                                        <  2:570563/141‑1 (MQ=255)
    aaTTGCCATTATTCTTAACTTTACGATTTATTCAAGGTTTAACTGGTGGTGGCGTCATCGTGATTGCAAAAGCCTCTGCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATg                                                                                                                                        <  4:155782/141‑1 (MQ=255)
      ttGCCATTATTCTTAACTTTACGATTTATTCAAGGTTTAACTGGTGGTGGCGTCATCGTGATTGCAAAAGCCTCTGCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGc                                                                                                                                      >  1:609999/1‑141 (MQ=255)
         ccATTATTCTTAACTTTACGATTTATTCAAGGTTTAACTGGTGGTGGCGTCATCGTGATTGCAAAAGCCTCTGCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGcatc                                                                                                                                   <  4:394679/141‑1 (MQ=255)
         ccATCATTCTTAACTTTACGATTTATTCTAGGTATAACTGGTGGTGGCGTCATCGTGATTGCAAAAGCCTCTGCTGGTGATAAACATAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGcatc                                                                                                                                   >  2:117152/1‑141 (MQ=255)
           attattCTTAACTTTACGATTTATTCAAGGTTTAACTGGTGGTGGCGTCATCGTGATTGCAAAAGCCTCTGCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGcatcat                                                                                                                                 >  4:227995/1‑141 (MQ=255)
              attCTTAACTTTACGATTTATTCAAGGTTTAACTGGTGGTGGCGTCATCGTGATTGCAAAAGCCTCTGCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCAc                                                                                                                              <  1:274938/141‑1 (MQ=255)
                tCTTAACTTTACGATTTATTCAAGGTTTAACTGGTGGTGGCGTCATCGTGATTGCAAAAGCCTCTGCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTa                                                                                                                            <  4:288048/141‑1 (MQ=255)
                       tttACGATTTATTCAAGGTTTAACTGGTGGTGGCGTCATCGTGATTGCAAAAGCCTCTGCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGc                                                                                                                     <  1:745386/141‑1 (MQ=255)
                        ttACGATTTATTCAAGGTTTAACTGGTGGTGGCGTCATCGTGATTGCAAAAGCCTCTGCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCa                                                                                                                    >  2:577201/1‑141 (MQ=255)
                           cGATTTATTCAAGGTTTAACTGGTGGTGGCGTCATCGTGATTGCAAAAGCCTCTGCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCa                                                                                                                 >  2:543519/1‑141 (MQ=255)
                           cGATTTATTCAAGGTTTAACTGGTGGTGGCGTCATCGTGATTGCAAAAGCCTCTGCTGGTGATAAAGTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCa                                                                                                                 >  2:543052/1‑141 (MQ=255)
                            gATTTATTCAAGGTTTAACTGGTGGTGGCGTCATCGTGATTGCAAAAGCCTCTGCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCAt                                                                                                                >  2:1082271/1‑141 (MQ=255)
                            gATTTATTCAAGGTTTAACTGGTGGTGGCGTCATCGTGATTGCAAAAGCCTCTGCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCAt                                                                                                                <  1:649757/141‑1 (MQ=255)
                             aTTTATTCAAGGTTTAACTGGTGGTGGCGTCATCGTGATTGCAAAAGCCTCTGCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCAtt                                                                                                               >  3:279069/1‑141 (MQ=255)
                              tttATTCAAGGTTTAACTGGTGGTGGCGTCATCGTGATTGCAAAAGCCTCTGCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTa                                                                                                              >  1:914194/1‑141 (MQ=255)
                                tATTCAAGGTTTAACTGGTGGTGGCGTCATCGTGATTGCAAAAGCCTCTGCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATGTTGAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGc                                                                                                            >  3:230692/1‑141 (MQ=255)
                                tATTCAAGGTTTAACTGGTGGTGGCGTCATCGTGATTGCAAAAGCCTCTGCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGc                                                                                                            >  1:305645/1‑141 (MQ=255)
                                tATTCAAGGTTTAACTGGTGGTGGCGTCATCGTGATTGCAAAAGCCTCTGCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGc                                                                                                            >  3:377636/1‑141 (MQ=255)
                                 aTTCAAGGTTTAACTGGTGGTGGCGTCATCGTGATTGCAAAAGCCTCTGCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGcc                                                                                                           >  1:235870/1‑141 (MQ=255)
                                 aTTCAAGGTTTAACTGGTGGTGGCGTCATCGTGATTGCAAAAGCCTCTGCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGcc                                                                                                           <  3:246021/141‑1 (MQ=255)
                                 aTTCAAGGTTTAACTGGTGGTGGCGTCATCGTGATTGCAAAAGCCTCTGCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGcc                                                                                                           <  2:703924/141‑1 (MQ=255)
                                 aTTCAAGGTTTAACTGGTGGTGGCGTCATCGTGATTGCAAAAGCCTCTGCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGcc                                                                                                           >  4:54233/1‑141 (MQ=255)
                                 aTTCAAGGTTTAACTGGTGGTGGCGTCATCGTGATTGCAAAAGCCTCTGCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGcc                                                                                                           <  1:144993/141‑1 (MQ=255)
                                  ttCACGGCTTAACTGGTGGTGGCGTCATCCTGACTGCTATCGCCTCTGCTTGTTATATATTTAGTGGCAACTCTCTTCCTTTATTTTTTGCAGCTTTAATGGTAGTTATCGTCATCATCACTATTCTTGCACCATTAGCCg                                                                                                          <  2:148852/141‑1 (MQ=255)
                                   tCAAGGTTTAACTGGTGGTGGCGTCATCGTGATTGCAAAAGCCTCTGCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCgg                                                                                                         <  2:277753/141‑1 (MQ=255)
                                     aaGGTTTAACTGGTGGTGGCGTCATCGTGATTGCAAAAGCCTCTGCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTg                                                                                                       >  2:456883/1‑141 (MQ=255)
                                      aGGTTTAACTGGTGGTGGCGTCATCGTGATTGCAAAAGCCTCTGCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTgg                                                                                                      >  1:607735/1‑141 (MQ=255)
                                      aGGTTTAACTGGTGGTGGCGTCATCGTGATTGCAAAAGCCTCTGCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTgg                                                                                                      >  1:172612/1‑141 (MQ=255)
                                        ttttAACTGGTGGTGGCGTCATCGTGATTGCAAAATCCTCTGCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTACTGGTAGTTATCGGCATCATCACTAGTCTTGCACCATTAGCCGGTGGAt                                                                                                    <  2:864490/140‑1 (MQ=255)
                                        gTTTAACTGGTGGTGGCGTCATCGTGATTGCAAAAGCCTCTGCTGGTGATAAAGTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGAt                                                                                                    <  1:374301/141‑1 (MQ=255)
                                            aaCTGGTGGTGGCGTCATCGTGATTGCAAAAGCCTCTGCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGc                                                                                                >  2:695420/1‑141 (MQ=255)
                                             aCTGGTGGTGGCGTCATCGTGATTGCAAAAGCCTCTGCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCt                                                                                               >  3:360226/1‑141 (MQ=255)
                                             aCTGGTGGTGGCGTCATCGTGATTGCAAAAGCCTCTGCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCt                                                                                               >  1:733044/1‑141 (MQ=255)
                                              cTGGTGGTGGCGTCATCGTGATTGCAAAAGCCTCTGCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTCGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCtt                                                                                              >  3:248531/1‑141 (MQ=255)
                                              cTGGTGGTGGCGTCATCGTGATTGCAAAAGCCTCTGCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCtt                                                                                              >  2:346372/1‑141 (MQ=255)
                                                     tggCGTCATCGTGATTGCAAAAGCCTCTGCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGt                                                                                       <  1:267935/141‑1 (MQ=255)
                                                      ggCGTCATCGTGATTGCAAAAGCCTCTGCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTa                                                                                      >  1:1041889/1‑141 (MQ=255)
                                                      ggCGTCATCGTGATTGCAAAAACCTCTGCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTa                                                                                      <  4:26540/141‑1 (MQ=255)
                                                       gCGTCATCGTGATTGCCAAAGCCTCTGCTGGTGATAAATTTAGTGGCAACGCACTAGCTAAATTTTTAGCATCTTTAATGGTAGTAAATGGCATCATCACTATTCTTGCACCATTAGCTGGTGGATTAGCTTTATCCGTAg                                                                                     >  3:218988/1‑141 (MQ=255)
                                                       gCGTCATCGTGATTGCAAAAGCCTCTGCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAg                                                                                     <  1:159692/141‑1 (MQ=255)
                                                          tCATCGTGATTGCAAAAGCCTCTGCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCaa                                                                                  >  4:146645/1‑141 (MQ=255)
                                                             tCGTGATTGCAAAAGCCTCTGCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTTATGGTAGTTAATGGCAGCATCACTATTCTTGCACCATTAGCTGGGGGATTAGCTTTATCCGTAGCAActt                                                                               >  4:130326/1‑139 (MQ=255)
                                                             tCGTGATTGCAAAAGCCTCTGCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACAt                                                                               <  4:13564/141‑1 (MQ=255)
                                                               gTGATTGCAAAAGCCTCTGCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATgg                                                                             >  1:1067173/1‑141 (MQ=255)
                                                                  aTTGCAAAAGCCTCTGCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGt                                                                          >  3:51778/1‑141 (MQ=255)
                                                                  aTTGCAAAAGCATCTGCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATCTTTAGAATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTGACCGATGGATTAGCTTTATCCGTAGCAACATGTCga                                                                          >  4:293719/1‑140 (MQ=255)
                                                                     gCAAAAGCCTCTGCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCt                                                                       <  2:261586/141‑1 (MQ=255)
                                                                      cAAAAGCCTCTGCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTa                                                                      >  3:274095/1‑141 (MQ=255)
                                                                        aaaGCCTCTGCTGGTGATAAATTTAGTGTCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTAtt                                                                    <  4:455574/141‑1 (MQ=255)
                                                                        aaaGCCTCTGCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTAtt                                                                    >  1:632158/1‑141 (MQ=255)
                                                                         aaGCCTCTGCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTCGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTAttt                                                                   >  1:853172/1‑141 (MQ=255)
                                                                         aaGCCTCTGCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTAttt                                                                   >  1:140137/1‑141 (MQ=255)
                                                                          tgCCTCTTCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTCGCCGGTGAATTAGCTTTATCCGTAGCAACATGGCGTTCTAtttt                                                                  <  2:942511/140‑1 (MQ=255)
                                                                                 gctgGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTAAATGGCATCATCACTATTCTTGCACCATTAGCTGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAAtt                                                           <  2:398346/141‑1 (MQ=255)
                                                                                   tgGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAAtttt                                                         <  2:368708/141‑1 (MQ=255)
                                                                                    ggTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTa                                                        <  2:707230/141‑1 (MQ=255)
                                                                                      tGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAAc                                                      >  4:314864/1‑141 (MQ=255)
                                                                                      tGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAAc                                                      <  1:217383/141‑1 (MQ=255)
                                                                                          aaaTTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTAtt                                                  >  3:110597/1‑141 (MQ=255)
                                                                                           aaTTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATtg                                                 <  3:428412/141‑1 (MQ=255)
                                                                                            aTTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATtgt                                                >  2:356323/1‑141 (MQ=255)
                                                                                              ttAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTgg                                              <  2:591787/141‑1 (MQ=255)
                                                                                                aGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCTCAATTTTAACTATTGTGGct                                            >  4:260280/1‑140 (MQ=255)
                                                                                                 gTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCAc                                           <  1:175711/141‑1 (MQ=255)
                                                                                                 gTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCAc                                           <  3:46000/141‑1 (MQ=255)
                                                                                                   ggCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACtc                                         >  2:1031410/1‑141 (MQ=255)
                                                                                                    gCAACGCGCTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACtca                                        <  2:954662/141‑1 (MQ=255)
                                                                                                     cAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACtcat                                       <  2:210964/141‑1 (MQ=255)
                                                                                                      aaCGCACTCGTTAAATTTTTAGCATCTTTAATGGTAGTTAATGTCATCATCACTATTCTTGCACCATTAGCCGGTAGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACAATTGTGGCACtcagc                                      <  2:119762/141‑3 (MQ=255)
                                                                                                      aaCGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACtcatc                                      <  3:122194/141‑1 (MQ=255)
                                                                                                      aaCGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACtcatc                                      <  2:165035/141‑1 (MQ=255)
                                                                                                      aaCGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACtcatc                                      <  2:528216/141‑1 (MQ=255)
                                                                                                             tCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCATCATTTTaa                               <  1:871810/141‑1 (MQ=255)
                                                                                                              cGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCATCATTTTAAt                              <  1:407669/141‑1 (MQ=255)
                                                                                                              cGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCATCATTTTAAt                              <  1:695277/141‑1 (MQ=255)
                                                                                                                  aaaTTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCATCATTTTAATTGGc                          <  3:227995/141‑1 (MQ=255)
                                                                                                                  aaaTTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCATCATTTTAATTGGc                          <  1:318316/141‑1 (MQ=255)
                                                                                                                  aaaTTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCATCATTTTAATTGGc                          <  1:760129/141‑1 (MQ=255)
                                                                                                                    aTTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCATCATTTTAATTGGCGt                        >  2:837183/1‑141 (MQ=255)
                                                                                                                    aTTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCATCATTTTAATTGGCGt                        <  3:146915/141‑1 (MQ=255)
                                                                                                                    aTTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCAGCATTTTAATTGGCGt                        <  1:791582/141‑1 (MQ=255)
                                                                                                                     tttttAGCATCTTTAATGGTAGTAAATGGCATCATCACTATTCTTGCACCATTAGCTGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCATCATTTTAATTGGCGTc                       <  1:471998/141‑1 (MQ=255)
                                                                                                                        ttAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCATCATTTTAATTGGCGTCGCt                    >  1:932944/1‑141 (MQ=255)
                                                                                                                        ttAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCATCATTTTAATTGGCGTCGCt                    >  1:932984/1‑141 (MQ=255)
                                                                                                                        ttAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCATCATTTTAATTGGCGTCGCt                    <  1:455406/141‑1 (MQ=255)
                                                                                                                         tAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCATCATTTTAATTGGCGTCGCtt                   <  2:66942/141‑1 (MQ=255)
                                                                                                                          aGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTGCACAATTTTCACTATTGTGGCACTCATCATTTTAATTGGCGTCGCTtc                  <  1:118148/141‑1 (MQ=255)
                                                                                                                           gCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCATCATTTTAATTGGCGTCGCTtct                 <  1:852132/141‑1 (MQ=255)
                                                                                                                             aTCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCATCATTTTAATTGGCGTCGCTTCTCa               >  3:158638/1‑141 (MQ=255)
                                                                                                                             aTCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCATCATTTTAATTGGCGTCGCTTCTCa               >  3:158749/1‑141 (MQ=255)
                                                                                                                                tttAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCATCATTTTAATTGGCGTCGCTTCTCAAtt            <  2:1015511/141‑1 (MQ=255)
                                                                                                                                  tAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCATCATTTTAATTGGCGTCGCTTCTCAATTAc          <  1:152113/141‑1 (MQ=255)
                                                                                                                                    aTGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCATCATTTTAATTGGCGTCGCTTCTCAATTACCt        <  4:54842/141‑1 (MQ=255)
                                                                                                                                    aTGGTAGTAAATGGCATCATCACTATTCTTGCACCATTAGCTGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCATCATTTTAATTGGCGTCGCTTCTCAATTACCt        <  4:377669/141‑1 (MQ=255)
                                                                                                                                       gTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCATCATTTTAATTGGCGTCGCTTCTCAATTACCTaaa     >  2:501584/1‑141 (MQ=255)
                                                                                                                                       gTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCATCATTTTAATTGGCGTCGCTTCTCAATTACCTaaa     <  2:395881/141‑1 (MQ=255)
                                                                                                                                         aGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCATCATTTTAATTGGCGTCGCTTCTCAATTACCTAAAAc   <  1:326582/141‑1 (MQ=255)
                                                                                                                                         aGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCATCATTTTAATTGGCGTCGCTTCTCAATTACCTAAAAc   <  3:146645/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                          
GATCAATTGCCATTATTCTTAACTTTACGATTTATTCAAGGTTTAACTGGTGGTGGCGTCATCGTGATTGCAAAAGCCTCTGCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTAGCATCTTTAATGGTAGTTAATGGCATCATCACTATTCTTGCACCATTAGCCGGTGGATTAGCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCATCATTTTAATTGGCGTCGCTTCTCAATTACCTAAAACA  >  USA300TCH1516_ALE/2558892‑2559170

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: