BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	182,889	C→T	5.7%	L318L (CTA→TTA)	glgA →	Glycogen synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	182,889	0	C	T	5.7%	431.1 / 7.4	140	L318L (CTA→TTA)	glgA	Glycogen synthase
Reads supporting (aligned to +/- strand): ref base C (60/70); new base T (6/3); total (66/74)
Fisher's exact test for biased strand distribution p-value = 3.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 140 total aligned reads displayed.

GACGAAACCAGTATCTATTAATTTTGAATATACAGTATCTAATAAAATTTTTGAATGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCAAAATGCAATTAAGGCGTCTAAAATTTTGAATTGGCAAATAGAGAGTGAACGGTTAG  >  USA300TCH1516_ALE/182750‑183027

 aCGAAATCATTATTTATTCATTTTGAATATACAGTATCTAATAAAATTTTTGCATGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTa                                                                                                                                          <  2:558629/141‑1 (MQ=255)

 aCGAAACCAGTATCTATTAATTTTGAATATACAGTATCTAATAAAATTTTTGAATGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTa                                                                                                                                          <  1:838280/141‑1 (MQ=255)

  cGAAACCAGTATCTATTAATTTTGAATATACAGTATCTAATAAAATTTTTGAATGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTaa                                                                                                                                         >  3:146763/1‑141 (MQ=255)

  cGAAACCAGTATCTATTAATTTTGAATATACAGTATCTAATAAAATTTTTGAATGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTaa                                                                                                                                         >  2:235867/1‑141 (MQ=255)

  cGAAACCAGTATCTATTAATTTTGAATATACAGTATCTAATAAAATTTTTGAATGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTaa                                                                                                                                         >  2:564055/1‑141 (MQ=255)

    aaaCCAGTATCTATTAATTTTGAATATACAGTATCTAATAAAATTTTTGAATGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAg                                                                                                                                       <  2:191729/141‑1 (MQ=255)

     aaCCAGTATCTATTAATTTTGAATATACAGTATCTAATAAAATTTTTGAATGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAgg                                                                                                                                      >  4:41715/1‑141 (MQ=255)

     aaCCAGTATCTATTAATTTTGAATATACAGTATCTAATAAAATTTTTGAATGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAgg                                                                                                                                      >  4:156681/1‑141 (MQ=255)

        cAGTATCTATTAATTTTGAATATACAGTATCTAATAAAATTTTTGAATGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAg                                                                                                                                   >  2:1033805/1‑141 (MQ=255)

        cAGTATCTATTAATTTTGAATATACAGTATCTAATAAAATTTTTGAATGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAg                                                                                                                                   <  3:100064/141‑1 (MQ=255)

          gTATCTATTAATTTTTAATATACAGTATCTAATAAAATTTTTGAATGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGtt                                                                                                                                 <  4:364461/141‑1 (MQ=255)

           tATCTATTAATTTTGAATATACAGTATCTAATAAAATTTTTGAATGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTa                                                                                                                                <  1:989207/141‑1 (MQ=255)

                aTTAATTTTGAATATACAGTATCTAATAAAATTTTTGAATGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCg                                                                                                                           <  1:1078212/141‑1 (MQ=255)

                aTTAATTTTGAATATACAGTATCTAATAAAATTTTTGAATGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCg                                                                                                                           <  3:451971/141‑1 (MQ=255)

                 ttAATTTTGAATATACAGTATCTAATAAAATTTTTGAATGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGt                                                                                                                          >  1:810148/1‑141 (MQ=255)

                 ttAATTTTGAATATACAGTATCTAATAAAATTTTTGAATGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGt                                                                                                                          >  1:985759/1‑141 (MQ=255)

                        tGAATATACAGTATCTAATAAAATTTTTTTTTTTTTTTTTGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTTTAAAGGAAGTTACGCCGTTAGAAAt                                                                                                                   >  2:497371/1‑141 (MQ=255)

                         gAATATACAGTATCTAATAAAATTTTTTTATTTTTTTTTGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCTTTGTTTTAAAAGAAGTTACGCCGTTAGAAAtt                                                                                                                  >  4:212952/1‑141 (MQ=255)

                         gAATATACAGTATCTAATAAAATTTTTGAATGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAAtt                                                                                                                  >  3:300637/1‑141 (MQ=255)

                         gAATATACAGTATCTAATAAAATTTTTGAATGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAAtt                                                                                                                  >  1:601351/1‑141 (MQ=255)

                             ataCAGTATCTAATAAAATTTTTGAATGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTTTAAAGGAAGTTACGCCGTTAGAAATTGaaa                                                                                                              >  1:519031/1‑141 (MQ=255)

                                cAGTATCTAATAAAATTTTTGAATGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTTTAAAGGAAGTTACGCCGTTAGAAATTGAAAAgg                                                                                                           >  1:296628/1‑141 (MQ=255)

                                cAGTATCTAATAAAATTTTTGAATGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTTTAAAGGAAGTTACGCCGTTAGAAATTGAAAAgg                                                                                                           >  1:296035/1‑141 (MQ=255)

                                 aGTATCTAATATAATTTTTGAATGTATACATGCTTGTTTACTAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTTAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGc                                                                                                          <  2:873063/141‑1 (MQ=255)

                                    aTCTAATAAAATTTTTGAATGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGt                                                                                                       <  2:726995/141‑1 (MQ=255)

                                     tCTAATAAAATTTTTGAATGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGtt                                                                                                      >  3:46371/1‑141 (MQ=255)

                                      cTAATAAAATTTTTGAATGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTa                                                                                                     <  1:987712/141‑1 (MQ=255)

                                       taataaAATTTTTGAATGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTTTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAg                                                                                                    <  2:605946/141‑1 (MQ=255)

                                       taataaAATTTTTGAATGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAg                                                                                                    <  2:1007875/141‑1 (MQ=255)

                                       taataaAATTTTTGAATGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAg                                                                                                    <  2:484833/141‑1 (MQ=255)

                                        aataaAATTTTTGAATGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTAAAATAAATAAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGa                                                                                                   <  2:969118/141‑1 (MQ=255)

                                        aataaAATTCTTGAATGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGa                                                                                                   >  1:955395/1‑141 (MQ=255)

                                         ataaAATTTTTGAATGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGaa                                                                                                  <  1:864385/141‑1 (MQ=255)

                                         ataaAATTTTTGAATGTATACATGCTGGGTTACCAGTAATTTTATCTCCTGTTAAAGAGCATATTTATCTCAATGAAAAATATCAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAAAAATTAAAAAGTCGGTTAGaa                                                                                                  >  2:431513/1‑141 (MQ=255)

                                           aaaaTTTTTGAATGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGaaaa                                                                                                <  2:663544/141‑1 (MQ=255)

                                                ttttGAATGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAag                                                                                           >  1:558220/1‑141 (MQ=255)

                                                 tttGAATGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAaga                                                                                          <  4:18081/141‑1 (MQ=255)

                                                   tGAATGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTTTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAgaga                                                                                        >  2:610575/1‑141 (MQ=255)

                                                   tGAATGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAgaga                                                                                        >  4:399791/1‑141 (MQ=255)

                                                     caTGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCAGTGTTCTAAGGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAACATTCAGAGATa                                                                                      >  2:754073/2‑141 (MQ=255)

                                                      aTGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATaa                                                                                     >  3:304985/1‑141 (MQ=255)

                                                      aTGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATaa                                                                                     >  1:653362/1‑141 (MQ=255)

                                                        gTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATc                                                                                   <  1:135992/141‑1 (MQ=255)

                                                         tataCATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCa                                                                                  <  1:435444/141‑1 (MQ=255)

                                                           taCATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACg                                                                                <  2:954286/141‑1 (MQ=255)

                                                            aCATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGa                                                                               <  2:557922/141‑1 (MQ=255)

                                                             cATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGAATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGAt                                                                              <  4:332823/141‑1 (MQ=255)

                                                               tGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGAttt                                                                            <  4:272467/141‑1 (MQ=255)

                                                                gCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTg                                                                           >  1:754598/1‑141 (MQ=255)

                                                                gCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATAAAGAGATAATCACGATTTg                                                                           >  1:554919/1‑141 (MQ=255)

                                                                   ggTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGttt                                                                        <  1:170137/141‑1 (MQ=255)

                                                                     tttACCAGTAATTTTCCCTCGTGTCAAAGAGCATATTTATCTCAATGAAAAAGATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTaa                                                                      <  1:332687/141‑1 (MQ=255)

                                                                         ccAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCAt                                                                  >  3:322949/1‑141 (MQ=255)

                                                                             tAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTAc                                                              <  4:310444/141‑1 (MQ=255)

                                                                             tAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTAc                                                              >  4:312588/1‑141 (MQ=255)

                                                                               aTTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGt                                                            <  1:249517/141‑1 (MQ=255)

                                                                                 tttATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCa                                                          >  2:1072843/1‑141 (MQ=255)

                                                                                 tttATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAACTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCa                                                          >  1:197783/1‑141 (MQ=255)

                                                                                  ttATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCaa                                                         >  1:219806/1‑141 (MQ=255)

                                                                                    aTCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCaaaa                                                       <  4:316411/141‑1 (MQ=255)

                                                                                     tctcCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCAAAAt                                                      >  4:362084/1‑141 (MQ=255)

                                                                                       tcCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCAAAATGc                                                    <  1:966946/141‑1 (MQ=255)

                                                                                                 gagCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCAAAATGCAATTAAGGCg                                          <  3:367326/141‑1 (MQ=255)

                                                                                                  agCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCAAAATGCAATTAAGGCGt                                         <  1:1033805/141‑1 (MQ=255)

                                                                                                  agCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCAAAATGCAATTAAGGCGt                                         <  3:368474/141‑1 (MQ=255)

                                                                                                  agCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCAAAATGCAATTAAGGCGt                                         >  2:370504/1‑141 (MQ=255)

                                                                                                   gCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATTATATACGTCGAAATGCAATTAAGGCGTc                                        <  2:1087273/141‑1 (MQ=255)

                                                                                                   gCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCAAAATGCAATTAAGGCGTc                                        >  2:444037/1‑141 (MQ=255)

                                                                                                   gCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCAAAATGCAATTAAGGCGTc                                        <  1:391460/141‑1 (MQ=255)

                                                                                                   gCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGGTAGAAATGTATAAGGCGGTTAGAAAATTAAGAGATAAGCACGATTTGTTTAATCATTTACGTCAAAATGCAATTAAGGCGTc                                        >  3:340896/1‑141 (MQ=255)

                                                                                                    cATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGCAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCAAAATGCAATTAAGGCGTCt                                       >  3:374141/1‑141 (MQ=255)

                                                                                                     atatTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCAAAATGCAATTAAGGCGTCTa                                      >  1:569750/1‑141 (MQ=255)

                                                                                                     atatTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCAAAATGCAATTAAGGCGTCTa                                      >  3:408305/1‑141 (MQ=255)

                                                                                                     atatTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCAAAATGCAATTAAGGCGTCTa                                      >  1:493251/1‑141 (MQ=255)

                                                                                                     atatTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCAAAATGCAATTAAGGCGTCTa                                      <  4:334141/141‑1 (MQ=255)

                                                                                                       atTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCAAAATGCAATTAAGGCGTCTaaa                                    >  2:254673/1‑141 (MQ=255)

                                                                                                               cAATGGAGAATGTAAATGTTGGAGTGTTCTAAGGGAGGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACAATTTGTTTAATCATTTACGTCAAAATGCAATTAAGGAGTATCCAATTTTGaa                            >  4:128263/1‑141 (MQ=255)

                                                                                                               cAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCAAAATGCAATTAAGGCGTCTAAAATTTTGaa                            <  1:246728/141‑1 (MQ=255)

                                                                                                                aaTGANAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGNCAAAATGCAATTAAGGCGTCTAAAATTTTGAAt                           <  1:438461/141‑1 (MQ=255)

                                                                                                                    aaaaaTATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCAAAATGCAATTAAGGCGTCTAAAATTTTGAATTGGc                       <  2:955395/141‑1 (MQ=255)

                                                                                                                    aaaaaTATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCAAAATGCAATTAAGGCGTCTAAAATTTTGAATTGGc                       <  1:546173/141‑1 (MQ=255)

                                                                                                                    aaaaaTATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCAAAATGCAATTAAGGCGTCTAAAATTTTGAATTGGc                       <  2:110085/141‑1 (MQ=255)

                                                                                                                    aaaaaTATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCAAAATGCAATTAAGGCGTCTAAAATTTTGAATTGGc                       <  3:262766/141‑1 (MQ=255)

                                                                                                                    aaaaaTATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCAAAATGCAATTAAGGCGTCTAAAATTTTGAATTGGc                       <  1:799101/141‑1 (MQ=255)

                                                                                                                    aaaaaTATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCAAAATGCAATTAAGGCGTCTAAAATTTTGAATTGGc                       >  2:641481/1‑141 (MQ=255)

                                                                                                                      aaaTATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCAAAATGCAATTAAGGCGTCTAAAATTTTGAATTGGCaa                     >  2:88952/1‑141 (MQ=255)

                                                                                                                      aaaTATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCAAAATGCAATTAAGGCGTCTAAAATTTTGAATTGGCaa                     >  4:375178/1‑141 (MQ=255)

                                                                                                                       aaTATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCAAAATGCAATTAAGGCGTCTAAAATTTTGAATTGGCaaa                    >  1:37739/1‑141 (MQ=255)

                                                                                                                        atatAAATTTGGCATTGTTTTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCAAAATGCAATTAAGGCGTCTAAAATTTTGAATTGGCAAAt                   <  1:824637/141‑1 (MQ=255)

                                                                                                                         tataAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCAAAATGCAATTAAGGCGTCTAAAATTTTGAATTGGCAAATa                  >  2:411979/1‑141 (MQ=255)

                                                                                                                         tataAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCAAAATGCAATTAAGGCGTCTAAAATTTTGAATTGGCAAATa                  >  1:1035794/1‑141 (MQ=255)

                                                                                                                         tataAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTAAGACTAAATGCAATTAAGGCGTCTAAAATTTTGAATTGGCAAATa                  >  1:556032/1‑141 (MQ=255)

                                                                                                                             aaTTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCAAAATGCAATTAAGGCGTCTAAAATTTTGAATTGGCAAATagaga              >  1:101218/1‑141 (MQ=255)

                                                                                                                                     aTTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCAAAATGCAATTAAGGCGTCTAAAATTTTGAATTGGCAAATAGAGAGTGAACgg      <  4:115948/141‑1 (MQ=255)

                                                                                                                                     aTTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCAAAATGCAATTAAGGCGTCTAAAATTTTGAATTGGCAAATAGAGAGTGAACgg      >  2:760839/1‑141 (MQ=255)

                                                                                                                                      ttGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCAAAATGCAATTAAGGCGTCTAAAATTTTGAATTGGCAAATAGAGAGTGAACGGt     <  3:50241/141‑1 (MQ=255)

                                                                                                                                       tGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCAAAATGCAATTAAGGCGTCTAAAATTTTGAATTGGCAAATAGAGAGTGAACGGtt    <  4:163926/141‑1 (MQ=255)

                                                                                                                                        gTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCAAAATGCAATTAAGGCGTCTAAAATTTTGAATTGGCAAATAGAGAGTGTACGGTta   <  3:402632/141‑1 (MQ=255)

                                                                                                                                         ttCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCAAAATGCAATTAAGGCGTCTAAAATTTTGAATTGGCAAATAGAGAGTGAACGGTtag  <  1:411979/141‑1 (MQ=255)

                                                                                                                                         ttCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCAAAATGCAATTAAGGCGTCTAAAATTTTGAATTGGCAAATAGAGAGTGAACGGTtag  <  2:61188/141‑1 (MQ=255)

GACGAAACCAGTATCTATTAATTTTGAATATACAGTATCTAATAAAATTTTTGAATGTATACATGCTGGTTTACCAGTAATTTTATCTCCTGTCAAAGAGCATATTTATCTCAATGAAAAATATAAATTTGGCATTGTTCTAAAGGAAGTTACGCCGTTAGAAATTGAAAAGGCGGTTAGAAAATTAAGAGATAATCACGATTTGTTTAATCATTTACGTCAAAATGCAATTAAGGCGTCTAAAATTTTGAATTGGCAAATAGAGAGTGAACGGTTAG  >  USA300TCH1516_ALE/182750‑183027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`