Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 2,867,125 A→G 5.7% L257L (TTA→CTA)  noc_2 ← Nucleoid occlusion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,867,1250AG 5.7% 395.5 / 9.1 134L257L (TTA→CTA) noc_2Nucleoid occlusion protein
Reads supporting (aligned to +/- strand):  ref base A (62/64);  new base G (2/6);  total (64/70)
Fisher's exact test for biased strand distribution p-value = 2.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 140 total aligned reads displayed.

GACAAAGCTGTTATGATCTTAGCTTTGTCTTTTATGTGTTAGTCATCATGTATACGACATCCTACTACTAACGTTTATATATTCGAATTTTTATTTCATAATAATCTTCATGATCTTTGTCTTTATGCTCAACATGTAATCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCTCATCTCGTGCTTGAGTGACATCTTGTGCAAAGCGAAGGTTTTGTGCTTTGACTTTTTCGGGCCCCGTTTTTTGGCGTACTCTATCTTCAGTCTGTTTCACAT  >  USA300TCH1516_ALE/2866987‑2867265
                                                                                                                                          |                                                                                                                                            
gACAAAGCTGTTATGATCTTAGCTTTGTCTTTTATGTGTTAGTCATCATGTATACGACATCCTACTACTAACGTTTATATATTCGAATTTTTATTTCATAATAATCTTCATGATCTTTGTCTTTATGCTCAACATGTAATc                                                                                                                                            <  2:181629/141‑1 (MQ=255)
gACAAAGCTATTATGATCTTAGCTTTGTCTTTTATGTGTTAGTCATCATGTATACGACATCCTACTACTAACGTTTATATATTCGAATTTTTATTTCATAATAATCTTCATGATCTTTGTCTTTATGCTCAACATGTAATc                                                                                                                                            >  1:746708/1‑141 (MQ=255)
 aCAACGCTGTTATGATCTTAGCTTTGTCTTTTATGTGTTAGTCATCATGTATACGACATCCTACTACTAACGTTTATATATTCGAATTTTTATTTCATAATAATCTTCATGATCTTTGTCTTTATGCTCAACATGTAATcc                                                                                                                                           >  1:304206/1‑141 (MQ=255)
       cTGTTATGATCTTAGCTTTGTCTTTTATGTGTTAGTCATCATGTATACGACATCCTACTACTAACGTTTATATATTCGAATTTTTATTTCATAATAATCTTCATGATCTTTGTCTTTATGCTCAACATGTAATCCtgtttg                                                                                                                                     <  1:384651/141‑1 (MQ=255)
        tGTTATGATCTTAGCTTTGTCTTTTATGTGTTAGTCATCATGTATACGACATCCTACTACTAACGTTTATATATTCGAATTTTTATTTCATAATAATCTTCATGATCTTTGTCTTTATGCTCAACATGTAATCCtgtttgt                                                                                                                                    <  1:1069520/141‑1 (MQ=255)
        tGTTATGATCTTAGCTTTGTCTTTTATGTGTTAGTCATCATGTATACGACATCCTACTACTAACGTTTATATATTCGAATTTTTATTTCATAATAATCTTCATGATCTTTGTCTTTATGCTCAACATGTAATCCtgtttgt                                                                                                                                    <  2:216292/141‑1 (MQ=255)
         tttATGATCTTAGCTTTGTCTTTTATGTGTTAGTCATCATGTATACGACATCCTACTACTAACGTTTATATATTCGAATTTTTATTTCATAATAATCTTCATGATCTTTGTCTTTATGCTCAACATGTAATCCtgtttgtt                                                                                                                                   <  2:67826/140‑1 (MQ=255)
         gTTATGATCTTAGCTTTGTCTTTTATGTGTTAGTCATCATGTATACGACATCCTACTACTAACGTTTATATATTCGAATTTTTATTTCATAATAATCTTCATGATCTTTGTCTTTATGCTCAACATGTAATCCtgtttgtt                                                                                                                                   <  1:803432/141‑1 (MQ=255)
          ttATGATCTTAGCTTTGTCTTTTATGTGTTAGTCATCATGTATACGACATCCTACTACTAACGTTTATATATTCGAATTTTTATTTCATAATAATCTTCATGATCTTTGTCTTTATGCTCAACATGTAGTCCTGTttgttg                                                                                                                                  <  1:262093/141‑1 (MQ=255)
          ttATGATCTTAGCTTTGTCTTTTATGTGTTAGTCATCATGTATACGACATCCTACTACTAACGTTTATATATTCGAATTTTTATTTCATAATAATCTTCATGATCTTTGTCTTTATGCTCAACATGTAATCCTGTttgttg                                                                                                                                  <  2:256523/141‑1 (MQ=255)
            aTGATCTTAGCTTTGTCTTTTATGTGTTAGTCATCATGTATACGACATCCTACTACTAACGTTTATATATTCGAATTTTTATTTCATAATAATCTTCATGATCTTTGTCTTTATGCTCAACATGTAATCCTGTTTGTTGaa                                                                                                                                >  3:159087/1‑141 (MQ=255)
            aTGATCTTAGCTTTGTCTTTTATGTGTTAGTCATCATGTATACGACATCCTACTACTAACGTTTATATATTCGAATTTTTATTTCATAATAATCTTCATGATCTTTGTCTTTATGCTCAACATGTAATCCTGTTTGTTGaa                                                                                                                                >  1:688626/1‑141 (MQ=255)
                   tAGCTTTGTCTTTTATGTGTTAGTCATCATGTATACGACATCCTACTACTAACGTTTATATATTCGAATTTTTATTTCATAATAATCTTCATGATCTTTGTCTTTATGCTCAACATGTAGTCCTGTTTGTTGAATCGCTTg                                                                                                                         <  1:39197/141‑1 (MQ=255)
                    aGCTTTGTCTTTTATGTGTTAGTCATCATGTATACGACATCCTACTACTAACGTTTATATATTCGAATTTTTATTTCATAATAATCTTCATGATCTTTGTCTTTATGCTCAACATGTAATCCTGTTTGTTGAATCGCTTgg                                                                                                                        <  1:122801/141‑1 (MQ=255)
                     gCTTTGTCTTTTATGTGTTAGTCATCATGTATACGACATCCTACTACTAACGTTTATATATTCGAATTTTTATTTCATAATAATCTTCATGATCTTTGTCTTTATGCTCAACATGTAATCCTGTTTGTTGAATCGCTTGGa                                                                                                                       >  1:139664/1‑141 (MQ=255)
                      cTTTGTCTTTTATGTGTTAGTCATCATGTATACGACATCCTACTACTAACGTTTATATATTCGAATTTTTATTTCATAATAATCTTCATGATCTTTGTCTTTATGCTCAACATGTAATCCTGTTTGTTGAATCGCTTGGAt                                                                                                                      >  2:1063083/1‑141 (MQ=255)
                           tCTTTTATGTGTTAGTCATCATGTATACGACATCCTACTACTAACGTTTATATATTCGAATTTTTATTTCATAATAATCTTCATGATCTTTGTCTTTATGCTCAACATGTAGTCCTGTTTGTTGAATCGCTTGGATACttt                                                                                                                 >  2:482756/1‑141 (MQ=255)
                               ttATGTGTTAGTCATCCTGTATACGACATCCTACTACTCACGTTTATATATTCGACTTTTTATTTCATAATAATCTTCATGATCTTTGTCTTTATGCTCAACATGTAATCCTGTTTGTTGAATCGCTTGGATACTTTTGcc                                                                                                             >  2:58508/1‑141 (MQ=255)
                                  tgtgTTAGTCATCATGTATACGACATCCTACTACTAACGTTTATATATTCGAATTTTTATTTCATAATAATCTTCATGATCTTTGTCTTTATGCTCAACATGTAATCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTAc                                                                                                          >  1:779249/1‑141 (MQ=255)
                                   gtgtTAGTCATCATGTATACGACATCCTACTACTAACGTTTATATATTCGAATTTTTATTTCATAATAATCTTCATGATCTTTGTCTTTATGCTCAACATGTAATCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTAcc                                                                                                         >  3:140178/1‑141 (MQ=255)
                                   gtgtTAGTCATCATGTATACGACATCCTACTACTAACGTTTATATATTCGAATTTTTATTTCATAATAATCTTCATGATCTTTGTCTTTATGCTCAACATGTAATCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTAcc                                                                                                         >  2:889534/1‑141 (MQ=255)
                                   gtgtTAGTCATCATGTATACGACATCCTACTACTAACGTTTATATATTCGAATTTTTATTTCATAATAATCTTCATGATCTTTGTCTTTATGCTCAACATGTAATCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTAcc                                                                                                         >  2:259369/1‑141 (MQ=255)
                                    tgtTAGTCATCATGTATACGACATCCTACTACTAACGTTTATATATTCGAATTTTTATTTCATAATAATCTTCATGATCTTTGTCTTTATGCTCAACATGTAATCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCt                                                                                                        <  2:835132/141‑1 (MQ=255)
                                    tgtTAGTCATCATGTATACGACATCCTACTACTAACGTTTATATATTCGAATTTTTATTTCATAATAATCTTCATGATCTTTGTCTGTATGCTCAACATGTAATCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCt                                                                                                        >  1:269683/1‑141 (MQ=255)
                                     gtTAGTCATCATTTATACGATATCCTACTACTAACGTTTATATATTCGAATTTTTATTTCATAATAATCTTCATGATCTTTGTCTCTATGCTCAACATGTAACCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCTc                                                                                                       <  2:227270/141‑1 (MQ=255)
                                      ttAGTCATCATGTATACGACATCCTACTACTAACGTTTATATATTCGAATTTTTATTTCATAATAATCTTCATGATCTTTGTCTTTATGCTCAACATGTAATCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCTct                                                                                                      >  2:632399/1‑140 (MQ=255)
                                       tAGTCATCATGTATACGACATCCTACTACTAACGTTTATATATTCGAATTTTTATTTCATAATAATCTTCATGATCTTTGTCTTTATGCTCAACATGTAATCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCTCAt                                                                                                     <  1:1031428/141‑1 (MQ=255)
                                          tcatcaTGTATACGACATGCTACTACTAACGTTTATATATTCGAATTTTTATTTCATAATAATCTTCATGATCTTTGTCTTTATGCTCAACATGTAATCCTGTTTGTGGAATCGCTTGGATACTTTTGCCTACCTCAtcgc                                                                                                  <  4:376763/141‑3 (MQ=255)
                                           catcatGTATACGACATCCTACTACTAACGTTTATATATTCGAATTTTTATTTCATAATAATCTTCATGATCTTTGTCTTTATGCTCAACATGTAATCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCTCATCTCg                                                                                                 <  2:552428/141‑1 (MQ=255)
                                           catcatGTATACGACATCCTACTACTAACGTTTATATATTCGAATTTTTATTTCATAATAATCTTCATGATCTTTGTCTTTATGCTCAACATGTAATCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCTCATCTCg                                                                                                 <  4:146082/141‑1 (MQ=255)
                                             tcatGTATACGACATCCTACTACTAACGTTTATATATTCGAATTTTTATTTCATAATAATCTTCATGATCTTTGTCTTTATGCTCAACATGTAATCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCTCATCTCGTg                                                                                               >  2:528437/1‑141 (MQ=255)
                                              catGTATACGACATCCTACTACTAACGTTTATATATTCGAATTTTTATTTCATAATAATCTTCATGATCTTTGTCTTTATTCTCAACATGTAATCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCTCATCTCGTGc                                                                                              <  4:377319/141‑1 (MQ=255)
                                                 gTATACGACATCCTACTACTAACGTTTATATATTCGAATTTTTATTTCATAATAATCTTCATGATCTTTGTCTTTATGCTCAACATGTAATCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCTCATCTCGTGCttt                                                                                           >  4:279375/1‑140 (MQ=255)
                                                 gTATACGACATCCTACTACTAACGTTTATATATTCGAATTTTTATTTCATAATAATCTTCATGATCTTTGTCTTTATGCTCAACATGTAATCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCTCATCTCGTGCTTg                                                                                           >  1:600582/1‑141 (MQ=255)
                                                    taCGACATCCTACTACTAACGTTTATATATTCGAATTTTTATTTCATAATAATCTTCATGATCTTTGTCTTTATGCTCAACATGTAATCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCTCATCTCGTGCTTGAGt                                                                                        <  2:100607/141‑1 (MQ=255)
                                                     aCGACATCCTACTACTAACGTTTATATATTCGAATTTTTATTTCATAATAATCTTCATGATCTTTGTCTTTATGCTCAACATGTAATCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCTCATCTCGTGCTTGAGTg                                                                                       <  2:871800/141‑1 (MQ=255)
                                                     aCGACATCCTACTACTAACGTTTATATATTAGAATTTTTATTTCATAATAATCTTCATGATCTTTGTCTTTATGCTCAACATGTAATCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCTCATCTCGTGCTTGAGTg                                                                                       >  2:372555/1‑141 (MQ=255)
                                                      cGACATCCTACTACTAACGTTTATATATTCGAATTTTTATTTCATAATAATCTTCATGATCTTTGTCTTTATGCTCAACATGTAATCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCTCATCTCGTGCTTGAGTGa                                                                                      >  1:817593/1‑141 (MQ=255)
                                                      cGACATCCTACTACTAACGTTTATATATTCGAATTTTTATTTCATAATAATCTTCATGATCTTTGTCTTTATGCTCAACATGTAATCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCTCATCTCGTGCTTGAGTGa                                                                                      >  1:1010562/1‑141 (MQ=255)
                                                      cGACATCCTACTACTAACGTTTATATATTCGAATTTTTATTTCATAATAATCTTCATGATCTTTGTCTTTATGCTCAACATGTAATCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCTCATCTCGTGCTTGAGTGa                                                                                      <  1:899747/141‑1 (MQ=255)
                                                       gACATCCTACTACTAACGTTTATATATTCGAATTTTTATTTCATAATAATCTTCATGATCTTTGTCTTTATGCTCAACATGTAATCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCTCATCTCGTGCTTGAGTGAc                                                                                     >  4:173064/1‑141 (MQ=255)
                                                        aCATCCTACTACTAACGTTTATATATTCGAATTTTTATTTCATAATAATCTTCATGATCTTTGTCTTTATGCTCAACATGTAATCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCTCATCTCGTGCTTGAGTGACa                                                                                    >  2:882456/1‑141 (MQ=255)
                                                        aCATCCTACTACTAACGTTTATATATTCGAATTTTTATTTCATAATAATCTTCATGATCTTTGTCTTTATGCTCAACATGTAATCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCTCATCTCGTGCTTGAGTGACa                                                                                    >  3:74947/1‑141 (MQ=255)
                                                          aTCCTACTACTAACGTTTATATATTCGAATTTTTATTTCATAATAGTCTTCATGATCTTTGTCTTTATGCTCAACATGTAATCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCTCATCTCGTGCTTGAGTGACATc                                                                                  <  3:68620/141‑1 (MQ=255)
                                                           tCCTACTACTAACGTTTATATATTCGAATTTTTATTTCATAATAATCTTCATGATCTTTGTCTTTATGCTCAACATGTAATCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCTCATCTCGTGCTTGAGTGACATCt                                                                                 <  2:661699/141‑1 (MQ=255)
                                                            ccTACTACTAACGTTTATATATTCGAATTTTTATTTCATAATAATCTTCATGATCTTTGTCTTTATGCTCAACATGTAATCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCTCATCTCGTGCTTGAGTGACATCtt                                                                                <  1:185210/141‑1 (MQ=255)
                                                             ctactactaACGTTTATATATTCGAATTTTTATTTCATAATAATCTTCATGATCTTTGTCTTTATGCTCAACATGTAATCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCTCATCTCGTGCTTGAGTGACATCTtg                                                                               >  2:32346/1‑141 (MQ=255)
                                                             ctactactaACGTTTATATATTCGAATTTTTATTTCATAATAATCTTCATGATCTTTGTCTTTATGCTCAACATGTAATCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCTCATCTCGTGCTTGAGTGACATCTtg                                                                               >  2:960396/1‑141 (MQ=255)
                                                              tactactaACGTTTATATATTCGAATTTTTATTTCATAATAATCTTCATGATCTTTGTCTTTATGCTCAACATGTAATCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCTCATCTCGTGCTTGAGTGACATCTtgt                                                                              >  1:60288/1‑141 (MQ=255)
                                                              tactactaACGTTTATATATTCGAATTTTTATTTCATAATAATCTTCATGATCTTTGTCTTTATGCTCAACATGTAATCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCTCATCTCGTGCTTGAGTGACATCTtgt                                                                              >  1:60456/1‑141 (MQ=255)
                                                                ctactaACGTTTATATATTCGAATTTTTATTTCATAATAATCTTCATGATCTTTGTCTTTATGCTCAACATGTAATCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCTCATCTCGTGCTTGAGTGACATCTTGTGc                                                                            >  2:984821/1‑141 (MQ=255)
                                                                  actaACGTTTATATATTCGAATTTTTATTTCATAATAATCTTCATGATCTTTGTCTTTATGCTCAACATGTAATCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCTCATCTCGTGCTTGAGTGACATCTTGTGCaa                                                                          >  2:268238/1‑141 (MQ=255)
                                                                   ctaACGTTTATATATTCGAATTTTTATTTCATAATAATCTTCATGATCTTTGTCTTTATGCTCAACATGTAATCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCTCATCTCGTGCTTGAGTGACATCTTGTGCaaa                                                                         <  2:985289/141‑1 (MQ=255)
                                                                      aCGTTTATATATTCGAATTTTTATTTCATAATAATCTTCATGAACTTTGTCTTTATGCTCAACATGTAATCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCTCATCTCGTGCTTGAGTGACATCTTGTGCAAAGCg                                                                      >  2:923385/1‑141 (MQ=255)
                                                                      aCGTTTATATATTCGAATGTTTATTTCATAATAATCTTCATGATCTTTGTCTTTATGCTCAACATGTAGTCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCTCATCTCGTGCTTGAGTGACATCTTGTGCAAAGCg                                                                      <  3:309664/141‑1 (MQ=255)
                                                                        gTTTATATATTCGAATTTTTATTTCATAATAATCTTCATGATCTTTGTCTTTATGCTCAACATGTAATCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCTCATCTCGTGCTTGAGTGACATCTTGTGCAAAGCGaa                                                                    <  4:380152/141‑1 (MQ=255)
                                                                             tatatTCGAATTTTTATTTCATAATAATCTTCATGATCTTTGTCTTTATGCTCAACATGTAATCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCTCATCTCGTGCTTGAGTGACATCTTGTGCAAAGCGAAGGttt                                                               >  4:139325/1‑141 (MQ=255)
                                                                             tatatTCGAATTTTTATTTCATAATAATCTTCATGATCTTTGTCTTTATGCTCAACATGTAATCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCTCATCTCGTGCTTGAGTGACATCTTGTGCAAAGCGAAGGttt                                                               >  1:632267/1‑141 (MQ=255)
                                                                             tatatTCGAATTTTTATTTCATAATAATCTTCATGATCTTTGTCTTTATGCTCAACATGTAATCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCTCATCTCGTGCTTGAGTGACATCTTGTGCAAAGCGAAGGttt                                                               >  1:364586/1‑141 (MQ=255)
                                                                               tatTCGAATTTTTATTTCATAATAATCTTCATGATCTTTGTCTTTATGCTCAACATGTAATCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCTCATCTCGTGCTTGAGTGACATCTTGTGCAAAGCGAAGGTTTtg                                                             <  3:287092/141‑1 (MQ=255)
                                                                                 ttCGAATTTTTATTTCATAATAATCTTCATGATCTTTGTCTTTATGCTCAACATGTAATCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCTCATCTCGTGCTTGAGTGACATCTTGTGCAAAGCGAAGGTTTtgtg                                                           >  3:5917/1‑141 (MQ=255)
                                                                                        ttttATTTCATAATAATCTGCATGATCTTTGTCTTTATGCTCAACATGTAATCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCTCATCTCGTGCTTGAGTGACATCTTGTGCAAAGCGAAGGTTTTGTGCTTTGAc                                                    <  2:566995/141‑1 (MQ=255)
                                                                                         tttATTTCATAATAATCTTCATGATCTTTGTCTTTATGCTCAACATGTAATCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCTCATCTCGTGCTTGAGTGACATCTTGTGCAAAGCGAAGGTTTTGTGCTTTGACt                                                   >  1:441377/1‑141 (MQ=255)
                                                                                           tATTTCATAATAATCTTCATGATCTTTGTCTTTATGCTCAACATGTAATCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCTCATCTCGTGCTTGAGTGACATCTTGTGCAAAGCGAAGGTTTTGTGCTTTGACttt                                                 <  2:409515/141‑1 (MQ=255)
                                                                                              ttCATAATAATCTTCATGATCTTTGTCTTTATGCTCAACATGTAATCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCTCATCTCGTGCTTGAGTGACATCTTGTGCAAAGCGAAGGTTTTGTGCTTTGACTTTTTc                                              >  2:433688/1‑141 (MQ=255)
                                                                                                cATAATAATCTTCATGATCTTTGTCTTTATGCTCAACATGTAATCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCTCATCTCGTGCTTGAGTGACATCTTGTGCAAAGCGAAGGTTTTGTGCTTTGACTTTTTCgg                                            <  2:1019749/141‑1 (MQ=255)
                                                                                                   aataatCTTCATGATCTTTGTCTTTATGCTCAACATGTAATCCTGTTTGTTGATTCGCTTGGATACTTTTGCCTACCTCATCTCGTGCTTGAGTGACATCTTGTGCAAAGCGAAGGTTTTGTGCTTTGACTTTTTCGGGcc                                         <  3:385180/141‑1 (MQ=255)
                                                                                                   aataatCTTCATGATCTTTGTCTTTATGCTCAACATGTAATCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCTCATCTCGTGCTTGAGTGACATCTTGTGCAAAGCGAAGGTTTTGTGCTTTGACTTTTTCGGGcc                                         <  1:984821/141‑1 (MQ=255)
                                                                                                    ataatCTTCATGATCTTTGTCTTTATGCTCAACATGTAGTCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCTCATCTCGTGCTTGAGTGACATCTTGTGCAAAGCGAAGGTTTTGTGCTTTGACTTTTTCGGGccc                                        <  2:132229/141‑1 (MQ=255)
                                                                                                    ataatCTTCATGATCTTTGTCTTTATGCTCAACATGTAATCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACTTCATCTCGTGCTTGAGTGACATCTTGTGCAAAGCGAAGGATTTGTGCTTTGACTTTTTCGGGccc                                        <  1:445933/141‑1 (MQ=255)
                                                                                                    ataatCTTCATGATCTTTGTCTTTATGCTCAACATGTAATCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCTCATCTCGTGCTTGAGTGACATCTTGTGCAAAGCGAAGGTTTTGTGCTTTGACTTTTTCGGGccc                                        <  1:207030/141‑1 (MQ=255)
                                                                                                    ataatCTTCATGATCTTTGTCTTTATGCTCAACATGTAATCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCTCATCTCGTGCTTGAGTGACATCTTGTGCAAAGCGAAGGTTTTGTGCTTTGACTTTTTCGGGccc                                        <  1:838454/141‑1 (MQ=255)
                                                                                                    ataatCTTCATGATCTTTGTCTTTATGCTCAACATGTAATCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCTCATCTCGTGCTTGAGTGACATCTTGTGCAAAGCGAAGGTTTTGTGCTTTGACTTTTTCGGGccc                                        <  3:243386/141‑1 (MQ=255)
                                                                                                        tCTTCATGATCTTTGTCTTTATGCTCAACATGTAATCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCTCATCTCGTGCTTGAGTGACATCTTGTGCAAAGCGAAGGTTTTGTGCTTTGACTTTTTCGGCCCCCGtt                                    >  2:173293/1‑141 (MQ=255)
                                                                                                          ttCATGATCTTTGTCTTTATGCTCAACATGTAGTCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCTCATCTCGTGCTTGAGTGACATCTTGTGCAAAGCGAAGGTTTTGTGCTTTGACTTTTTCGGGCCCCGtttt                                  >  2:945292/1‑141 (MQ=255)
                                                                                                          ttCATGATCTTTGTCTTTATGCTCAACATGTAATCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCTCATCTCGTGCTTGAGTGACTTCTTGTGCAAAGCGAAGGTTTTGTGCTTTGACTTTTTCGGGCCCCGtttt                                  >  2:2044/1‑141 (MQ=255)
                                                                                                                aTCTTTGTCTTTATGCTCAACATGTAATCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCTCATCTCGTGCTTGAGTGACATCTTGTGCAAAGCGAAGGTTTTGTGCTTTGACTTTTTCGGGCCCCGTTTTTTGGCg                            <  2:688393/141‑1 (MQ=255)
                                                                                                                aTCTTTGTCTTTATGCTCAACATGTAATCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCTCATCTCGTGCTTGAGTGACATCTTGTGCAAAGCGAAGGTTTTGTGCTTTGACTTTTTCGGGCCCCGTTTTTTGGCg                            <  4:193916/141‑1 (MQ=255)
                                                                                                                   tttGTCTTTATGCTCAACATGTAGTCCTGCGTTTTGAATCTCTTTGATACTTTTGCCTACCTCATCTCGTGCTTGAGTGACATCTTGTGCAAAGCGAAGGTTTTGTGCTTTGACTTTTTCGGGCCCCGTTTTTTGGCGTAc                         <  1:482756/141‑1 (MQ=255)
                                                                                                                    ttGTCTTTATGCTCAACATGTAATCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCTCATCTCGTGCTTGAGTGACATCTTGTGCAAAGCGAAGGTTTTGTGCTTTGACTTTTTCGGGCCCCGTTTTTTGGCGTAct                        >  1:74605/1‑141 (MQ=255)
                                                                                                                     tGTCTTTATGCTCAACATGTAATCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCTCATCTCGTGCTTGAGTGACATCTTGTGCAAAGCGAAGGTTTTGTGCTTTGACTTTTTTGTGTTTCGTTTTTTGGCGTAatc                       >  4:360364/1‑141 (MQ=255)
                                                                                                                       tCTTTATGCTCAACATGTAATCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCTCATCTCGTGCTTGAGTGACATCTTGTGCAAAGCGCAGGTTTTGTGCTTTGACTTTTTCGGGCCCCGTTTTTTGGCGTACTCTa                     >  4:230114/1‑141 (MQ=255)
                                                                                                                       tCTTTATGCTCAACATGTAATCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCTCATCTCGTGCTTGAGTGACATCTTGTGCAAAGCGAAGGTTTTGTGCTTTGACTTTTTCGGGCCCCGTTTTTTGGCGTACTCTa                     >  3:26802/1‑141 (MQ=255)
                                                                                                                        cTTTATGCTCAACATGTAATCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCTCATCTCGTGCTTGAGTGACATCTTGTGCAAAGCGAAGGTTTTGTGCTTTGACTTTTTCGGGCCCCGTTTTTTGGCGTACTCTAt                    <  4:48052/141‑1 (MQ=255)
                                                                                                                           tATGCTCAACATGTAATCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCTCATCTCGTGCTTGAGTGACATCTTGTGCAAAGCGAAGGTTTTGTGCTTTGACTTTTTCGGGCCCCGTTTTTTGGCGTCCTCTATCtt                 >  1:159801/1‑141 (MQ=255)
                                                                                                                           tATGCTCAACATGTAATCCTGCTTGTTGAATCGCTTGGATACTTTTGCCTACCTCTTCTCGTGCTTGATTGACATCTTGTGCAAAGCGAAGGTCTTGTGCTTTGACTTTTTCTGGCCCCGTTTTTTGGCGTACTCTCTCtt                 >  4:322668/1‑141 (MQ=255)
                                                                                                                               cTCAACATGTAGTCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCTCATCTCGTGCTTGAGTGACATCTTGTGCAAAGCGAAGGTTTTGTGCTTTGACTTTTTCGGGCCCCGTTTTTTGGCGTACTCTATCTTCAGt             <  4:65724/141‑1 (MQ=255)
                                                                                                                                 cAACATGTAATCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCTCATCTCGTGCTTGAGTGACATCTTGTGCAAAGCGAAGGTTTTGTGCTTTGACTTTTTCGGGCCCCGTTTTTTGGCGTACTCTATCTTCAGTCt           >  1:31641/1‑141 (MQ=255)
                                                                                                                                 cAACATGTAATCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCTCATCTCGTGCTTGAGTGACATCTTGTGCAAAGCGAAGGTTTTGTGCTTTGACTTTTTCGGGCCCCGTTTTTTGGCGTACTCTATCTTAAATCt           <  2:331510/141‑1 (MQ=255)
                                                                                                                                  aaCATATAATCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCTCATCTCGTGCTTGAGTGACATCTTGTGCAAAGCGAAGGTTTTGTGCTTTGACTTTTTCGGGCCCCGTTTTTTGGCGTACTCTATCTTCAGTCTg          <  1:908361/141‑1 (MQ=255)
                                                                                                                                   aCATGTAATCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCTCATCTCGTGCTTGAGTGACATCTTGTGCAAAGCGAAGGTTTTGTGCTTTGACTTTTTCGGGCCCCGTTTTTTGGCGTACTCTATCTTCAGTCTGt         <  3:876/141‑1 (MQ=255)
                                                                                                                                   aCATGTAATCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCTCATCTCGTGCTTGAGTGACATCTTGTGCAAAGCGAAGGTTTTGTGCTTTGACTTTTTCGGGCCCCGTTTTTTGGCGTACTCTATCTTCAGTCTGt         <  3:254048/141‑1 (MQ=255)
                                                                                                                                    cATGTAATCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCTCATCTCGTGCTTGAGTGACATCTTGTGCAAAGCGAAGGTTTTGTGCTTTGACTTTTTCGGGCCCCGTTTTTTGGCGTACTCTATCTTCAGTCTGtt        <  2:123063/141‑1 (MQ=255)
                                                                                                                                        tAATCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCTCATCTCGTGCTTGAGTGACATCTTGTGCAAAGCGAAGGTTTTGTGCTTTGACTTTTTCGGGCCCCGTTTTTTGGCGTACTCTATCTTCAGTCTGTTTcac    <  2:685889/141‑1 (MQ=255)
                                                                                                                                         aaTCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCTCATCTCGTGCTTGAGTGACATCTTGTGCAAAGCGAAGGTTTTGTGCTTTGACTTTTTCGGGCCCCGTTTTTTGGCGTACTCTATCTTCAGTCTGTTTcaca   >  4:158534/1‑141 (MQ=255)
                                                                                                                                         aaTCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCTCATCTCGTGCTTGAGTGACATCTTGTGCAAAGCGAAGGTTTTGTGCTTTGACTTTTTCGGGCCCCGTTTTTTGGCGTACTCTATCTTCAGTCTGTTTcaca   >  4:265603/1‑141 (MQ=255)
                                                                                                                                         aaTCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCTCATCTCGTGCTTGAGTGACATCTTGTGCAAAGCGAAGGTTTTGTGCTTTGACTTTTTCGGGCCCCGTTTTTTGGCGTACTCTATCTTCAGTCTGTTTcaca   >  1:941901/1‑141 (MQ=255)
                                                                                                                                          aTCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCTCATCTCGTGCTTGAGTGACATCTTGTGCAAAGCGAAGGTTTTGTGCTTTGACTTTTTCGGGCCCCGTTTTTTGGCGTACTCTATCTTCAg              <  2:797287/129‑1 (MQ=255)
                                                                                                                                          aTCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCTCATCTCGTGCTTGAGTGACATCTTGTGCAAAGCGAAGGTTTTGTGCTTTGACTTTTTCGGGCCCCGTTTTTTGGCGTACTCTATCTTCAg              >  1:797287/1‑129 (MQ=255)
                                                                                                                                          aTCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCCAACCGGGTGCTTGAGTGACATCTTGTGCAAAGCGAAGGTTTTGTGCTTTGACTTTTTCGGGCCCCGTTTTTTGGCGTACTCTATCTTCAGTCTGTTTCACAt  <  1:9943/141‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                            
GACAAAGCTGTTATGATCTTAGCTTTGTCTTTTATGTGTTAGTCATCATGTATACGACATCCTACTACTAACGTTTATATATTCGAATTTTTATTTCATAATAATCTTCATGATCTTTGTCTTTATGCTCAACATGTAATCCTGTTTGTTGAATCGCTTGGATACTTTTGCCTACCTCATCTCGTGCTTGAGTGACATCTTGTGCAAAGCGAAGGTTTTGTGCTTTGACTTTTTCGGGCCCCGTTTTTTGGCGTACTCTATCTTCAGTCTGTTTCACAT  >  USA300TCH1516_ALE/2866987‑2867265

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: