Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 220,088 A→T 5.2% G289G (GGT→GGA ipdC ← Indole‑3‑pyruvate decarboxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE220,0880AT 5.2% 445.6 / 10.0 143G289G (GGT→GGAipdCIndole‑3‑pyruvate decarboxylase
Reads supporting (aligned to +/- strand):  ref base A (74/61);  new base T (5/3);  total (79/64)
Fisher's exact test for biased strand distribution p-value = 7.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.45e-01

Only 100 of 153 total aligned reads displayed.

GTTGCGTTATTCGTATATGAAATATTGGATAACTGTTTTAACAATGATGGTAGAGATATTTCATCATTTGTAACATCGTCAATTTTGATATTGTGATGATTTAACATAACGACATCATCGATATTGAATTGGTATGAAAAACCTGCTGTTGCTGAATCTGTTAATTTGGCTCCAATATTTAAAATTAAATCGCTGTTGTCCACATAATCTCGTATTTTATCTTCGGCAATTTTCCCATCGTAAATACCCATATAATATGGATTTTCCTCATTAAAAGCACC  >  USA300TCH1516_ALE/219948‑220228
                                                                                                                                            |                                                                                                                                            
gTTGCGTTATTCGTATATGGAATATTGGATAACTGTTTTAACAATGATGGTAGAGATATTTCATCATTTGTAACATCGTCAATTTTGATATTGTGATGGTTTAACCTAACGACATCATCGATATTGAATTGGTATGaaaaa                                                                                                                                              <  1:933203/141‑1 (MQ=255)
 ttGCGTTATTCGTATATGAAATATTGGATAACTGTTTTAACAATGATGGTAGAGATATTTCATCATTTGTAACATCGTCAATTTTGATATTGTGATGATTTAACATAACGACATCATCGATATTGAATTGGTATGAAAAAc                                                                                                                                             <  2:716319/141‑1 (MQ=255)
   gCGTTATTCGTATATGAAATATTGGATAACTGTTTTAACAATGATGGTAGAGATATTTCATCATTTGTAACATCGTCAATTTTGATATTGTGATGATTTAACATAACGACATCATCGATATTGAATTGGTATGAAAAACct                                                                                                                                           >  3:275873/1‑141 (MQ=255)
   gCGTTATTCGTATATGAAATATTGGATAACTGTTTTAACAATGATGGTAGAGATATTTCATCATTTGTAACATCGTCAATTTTGAAAGTGTGATGATTTAACATAACGACATCATCGATATTGAATTGGTATGAAAAACct                                                                                                                                           <  1:837185/141‑1 (MQ=255)
      ttATTCGTATATGAAATATTGGATAACTGTTTTAACAATGATGGTAGAGATATTTCATCATTTGTCACATCGTCGGCTTTGAGATGGTGATGATTTAACATAACGACATCATCGGTATTGAATTGGTATGAAAAACctgct                                                                                                                                        >  1:269819/1‑141 (MQ=255)
       tgttCGTATATGAAATATTGGATAACTGTTTTAACAACGATGGTAGAGATATTTCATCATTTGTAACATCATCAATTTTGATATTGTGATGATTTAACATAACGACATCATCGATATTGAATTGGTATGAAAATCctgctg                                                                                                                                       <  1:732533/139‑1 (MQ=255)
         ttCGTATATGAAATATTGGATAACTGTTTTAACAATGATGGTAGAGATATTTCATCATTTGTAACATCGTCAATTTTGATATTGTGATGATTTAACATAACGACATCATCGATATTGAATTGGTATGAAAAACCTGCTGtt                                                                                                                                     <  2:582285/141‑1 (MQ=255)
         ttCGTATATGAAATATTGGATAACTGTTTTAACAATGATGGTAGAGATATTTCATCATTTGTAACATCGTCAATTTTGATATTGTGATGATTTAACATAACGACATCATCGATATTGAATTAGTATGAAAAACCTGCTGtt                                                                                                                                     <  4:380161/141‑1 (MQ=255)
          tCGTATATGAAATATTGGATAACTGTTTTAACAATGATGGTAGAGATATTTCATCATTTGTAACATCGTCAATTTTGATATTGTGATGATTTAACATAACGACATCATCGATATTGAATTGGTATGAAAAACCTGCTGTTg                                                                                                                                    <  1:800028/141‑1 (MQ=255)
          tCGTATATGAAATATTGGATAACTGTTTTAACAATGATGGTAGAGATATTTCATCATTTGTAACATCGTCAATTTTGATATTGTGATGATTTAACATAACGACATCATCGATATTGAATTGGTATGAAAAACCTGCTGTTg                                                                                                                                    <  1:504174/141‑1 (MQ=255)
             tataTGAAATATTGGATAACTGTTTTAACAATGATGGTAGAGATATTTCATAATTTGTAACATCGTCAATTTTGATATTGTGATGATTTAACATAACGACATCATCGATATTGAATTGGTATGAAAAACCTGCTGTTGCTg                                                                                                                                 <  4:253220/141‑1 (MQ=255)
              atatGAAATATTGGATAACTGTTTTAACAATGATGGTAGAGATATTTCATCATTTGTAACATCGTCAATTTTGATATTGTGATGATTTAACATAACGACATCATCGATATTGAATTGGTATGAAAAACCTGCTGTTGCTGa                                                                                                                                <  2:235885/141‑1 (MQ=255)
                  gAAATATTGGATAACTGTTTTAACAATGATGGTAGAGATATTTCATCATTTGTAACATCGTCAATTTTGATATTGTGATGATTTAACATAACGACATCATCGATATTGAATTGGTATGAAAAACCTGCTGTTGCTGAATCt                                                                                                                            <  4:362339/141‑1 (MQ=255)
                    aaTATTGGATAACTGTTTTAACAATGATGGTAGAGATATTTCATCATTTGTAACATCGTCAATTTTGATATTGTGATGATTTAACATAACGACATCATCGATATTGAATTGGTATGAAAAACCTGCTGTTACTGAATCTGt                                                                                                                          >  2:941039/1‑141 (MQ=255)
                     atatTGGATAACTGTTTTAACAATGATGGTAGAGATATTTCATCATTTGTAACATCGTCAATTTTGATATTGTGATGATTTAACATAACGACATCATCGATATTGAATTGGTATGAAAAACCTGCTGTTGCTGAATCTGtt                                                                                                                         >  1:278384/1‑141 (MQ=255)
                      tatTGGATAACTGTTTTAACAATGATGGTAGAGATATTTCATCATTTGTAACATCGTCAATTTTGATATTGTGATGATTTAACATAACGACATCATCGATATTGAATTGGTATGAAAAACCTGCTGTTGCTGAATCTGTTa                                                                                                                        >  1:429891/1‑141 (MQ=255)
                      tatTGGATAACTGTTTTAACAATGATGGTAGAGATATTTCATCATTTGTAACATCGTCAATTTTGATATTGTGATGATTTAACATAACGACATCATCGATATTGAATTGGTATGAAAAACCTGCTGTTGCTGAATCTGTTa                                                                                                                        <  1:600653/141‑1 (MQ=255)
                       atTGGATAACTGTTTTAACAATGATGGTAGAGATATTTCATCATTTGTAACATCGTCAATTTTGATATTGTGATGATTTAACATAACGACATCATCGATATTGAATTGGTATGAAAAACCTGCTGTTGCTGAATCTGTTaa                                                                                                                       >  1:698948/1‑141 (MQ=255)
                        ttGGATAACTGTTTTAACAATGATGGTAGAGATATTTTATCATTTGTATCAACGTCATTCTTGATATAGAGATGATTTAAGATAACGACATCATAGCTATTGAATTGGTATGAACATCCTGCTGTTGCTGAATCTGTTAAt                                                                                                                      >  4:52128/1‑141 (MQ=255)
                          ggATAACTGTTTTAACAATGATGGTAGAGATATTTCATCATTTGTAACATCGTCAATTTTGATATTGTGATGATTTAACATAACGACATCATCGATATTGAATTGGTATGAAAAACCTGCTGTTGCTGAATCTGTTAAttt                                                                                                                    >  2:559910/1‑141 (MQ=255)
                          ggATAACTGTTTTAACAATGATGGTAGAGATATTTCATCATTTGTAACATCGTCAATTTTGATATTGTGATGATTTAACATAACGACATCATCGATATTGAATTGGTATGAAAAACCTGCTGTTGCTGAATCTGTTAAttt                                                                                                                    >  2:621429/1‑141 (MQ=255)
                          ggATAACTGTTTTAACAACGATGGTAGAGATATTTCATCATTTGTAACATCATCAATTTTGATATTGTGATGATTTAACATAACGACATCATCGATATTGAATTGGTATGAAAATCCTGCTGTTGCTGAATCTGTTAAttt                                                                                                                    >  4:59455/1‑141 (MQ=255)
                            aTAACTGTTTTAACAATGATGGTATAGATATTTCATCATTTGTAACATCGTCAATTTTGATATTGTGATGATTTAACATAACGACATCATCGATATTGAATTGGTATGAAAAACCTGCTGTTGCTGAATCTGTTAATTTgg                                                                                                                  <  4:429201/141‑1 (MQ=255)
                            aTAACTGTTTTAACAATGATGGTAGAGATATTTCATCATTTGTAACATCGTCAATTTTGATATTGTGATGATTTAACATAACGACATCATCGATATTGAATTGGTATGAAAAACCTGCTGTTGCTGAATCTGTTAATTTgg                                                                                                                  >  2:549658/1‑141 (MQ=255)
                            aTAACTGTTTTAACAATGATGGTAGAGATATCTCATCATTTGTAACATCGTCAATTTTGATATTGTGATGATTTAACATAACGACATCATCGATATTGAATTGGTATGAAAAACCTGCTGTTGCTGAATCTGTTAATTTgg                                                                                                                  <  2:924600/141‑1 (MQ=255)
                             tAACTGTTTTAACAATGATGGTAGAGATATTTCATCATTTGTAACATCGTCAATTTTGATATTGTGATGATTTAACATAACGACATCATCGATATTGAATTGGTATGAAAAACCTGCTGTTGCTGAATCTGTTAATTTGGc                                                                                                                 >  4:385154/1‑141 (MQ=255)
                                cTGTTTTAACAATGATGGTAGAGATATTTCATCATTTGTAACATCGTCAATTTTGATATTGTGATGATTTAACATAACGACATCATCGATATTGAATTGGTATGAAAAACCTGCTGTTGCTGAATCTGTTAATTTGGCTcc                                                                                                              <  2:374002/141‑1 (MQ=255)
                                   ttttAACAACGATGGTAGAGATATTTCATCATTTGTAACATCATCAATTTTGATATTGTGATGATTTAACATAACGACATCATCGATATTGAATTGGTATGAAAATCCTGCTGTTGCTGAATCTGTTAATTTGGCTCCAat                                                                                                           >  2:724505/1‑141 (MQ=255)
                                     ttAACAATGATGGTAGAGATATTTCATCATTTGTAACATCGTCAATTTTGATATTGTGATGATTTAACATAACGACATCATCGATATTGAATTGGTATGAAAAACCTGCTGTTGCTGAATCTGTTAATTTGGCTCCAatat                                                                                                         >  1:996189/1‑141 (MQ=255)
                                     ttAACAATGATGGTAGAGATATTTCATCATTTGTAACATCGTCAATTTTGATATTGTGATGATTTAACATAACGACATCATCGATATTGAATTGGTATGAAAAACCTGCTGTTGCTGAATCTGTTAATTTGGCTCCAatat                                                                                                         <  2:317060/141‑1 (MQ=255)
                                      tAACAATGATGGTAGAGATATTTCATCATTTGTAACATCGTCAATTTTGATATTGTGATGATTTAACATAACGACATCATCGATATTGAATTGGTATGAAAAACCTGCTGTTGCTGAATCTGTTAATTTGGCTCCAATAtt                                                                                                        <  3:241954/141‑1 (MQ=255)
                                      tAACAATGATGGTAGAGATATTTCATCATTTGTAACATCGTCAATTTTGATATTGTGATGATTTAACATAACGACATCATCGATATTGAATTGGTATGAAAAACCTGCTGTTGCTGAATCTGTTAATTTGGCTCCAATAtt                                                                                                        <  1:176520/141‑1 (MQ=255)
                                       aaCAATGATGGTAGAGATATTTCATCATTTGTAACATCGTCAATTTTGATATTGTGATGATTTAACATAACGACATCATCGATATTGAATTGGTATGAAAAACCTGCTGTTGCTGAATCTGTTAATTTGGCTCCAATAttt                                                                                                       <  1:1060840/141‑1 (MQ=255)
                                         cAATGATGGTAGAGATATTTCATCATTTGTAACATCGTCAATTTTGATATTGTGATGATTTAACATAACGACATCATCGATATTGAATTGGTATGAAAAACCTGCTGTTGCTGAATCTGTTAATTTGGCTCCAATATTTaa                                                                                                     >  2:281755/1‑141 (MQ=255)
                                         cAATGATGGTAGAGATATTTCATCATTTGTAACATCGTCAATTTTGATATTGTGATGATTTAACATAACGACATCATCGATATTGAATTGGTATGAAAAACCTGCTGTTGCTGAATCTGTTAATTTGGCTCCAATATTTaa                                                                                                     >  1:1082739/1‑141 (MQ=255)
                                           atgatgGTAGAGATATTTCATCATTTGTAACATCGTCAATTTTGATATTGTGATGATTTAACATAACGACATCATCGATATTGAATTGGTATGAAAAACCTGCTGTTGCTGAATCTGTTAATTTGGCTCCAATATTTaaaa                                                                                                   >  2:1079215/1‑141 (MQ=255)
                                            tgatgGTAGAGATATTTCATCATTTGTAACATCGTCAATTTTGATATTGTGATGATTTAACATAACGACATCATCGATATTGAATTGGTATGAAAAACCTGCTGTTGCTGAATCTGTTAATTTGGCTCCAATATTTAAAAt                                                                                                  <  1:111076/141‑1 (MQ=255)
                                              atgGTAGAGATATTTCATCATTTGTAACATCGTCAATTTTGATATTGTGATGATTTAACATAACGACATCATCGATATTGAATTGGTATGAAAAACCTGCTGTTGCTGAATCTGTTAATTTGGCTCCAATATTTAAAATTa                                                                                                >  3:41648/1‑141 (MQ=255)
                                               tgGTAGAGATATTTCATCATTTGTAACATCGTCAATTTTGATATTGTGATGATTTAACATAACGACATCATCGATATTGAATTGGTATGAAAAACCTGCTGTTGCTGAATCTGTTAATTTGGCTCCAATATTTAAAATTaa                                                                                               >  1:1043861/1‑141 (MQ=255)
                                               tgGTACAGATATTTCATCATTTGTAACATCGTCAATTTTGATATTGTGATGATTTAACATAACGACATCATCGATATTGAATTGGTATGAAAGACCTGCTGTTGCTGAATCTGTTAATTTGGCTCCAATATTTAAAATTaa                                                                                               <  1:811633/141‑1 (MQ=255)
                                                ggTAGAGATATTTCATCATTTGTAACATCGTCAATTTTGATATTGTGATGATTTAACATAACGACATCATCGATATTGAATTGGTATGAAAAACCTGCTGTTGCTGAATCTGTTAATTTGGCTCCAATATTTAAAATTaaa                                                                                              >  3:17128/1‑141 (MQ=255)
                                                    gagaTATTTCATCATTTGTAACATCGTCAATTTTGATATTGTGATGATTTAACATAACGACATCATCGATATTGAATTGGTATGAAAAACCTGCTGTTGCTGAATCTGTTAATTTGGCTCCAATATTTAAAATTAAATCGc                                                                                          <  2:545753/141‑1 (MQ=255)
                                                     agaTATTTCATCATTTGTAACATCGTCAATTTTGATATTGTGATGATTTAACATAACGACATCATCGATATTGAATTGGTATGAAAAACCTGCTGTTGCTGAATCTGTTAATTTGGCTCCAATATTTAAACTTAAATCGCt                                                                                         >  2:157468/1‑141 (MQ=255)
                                                     agaTATTTCATCATTTGTAACATCGTCAATTTTGATATTGTGATGATTTAACATAACGACATCATCGATATTGAATTGGTATGAAAAACCTGCTGTTGCTGAATCTGTTAATTTGGCTCCAATATTTAAAATTAAATCGCt                                                                                         >  1:60031/1‑141 (MQ=255)
                                                          tttCATCATTTGTAACATCGTCAATTTTGATATTGTGAGGATTTAACATAACGACATCATCGATATTGAATTGGTATGAAAAACCTGCTGTTGCTGAATCTGTTAATTTGGCTCCAATATTTAAAATTAAATCGCtgttgt                                                                                    >  4:207407/1‑141 (MQ=255)
                                                             catcatTTGTAACATCGTCAATTTTGATATTGTGATGATTTAACATAACGACATCATCGATATTGAATTGGTATGAAAAACCTGCTGTTGCTGAATCTGTTAATTTGGCTCCAATATTTAAAATTAAATCGCTGTTGTCca                                                                                 <  1:1011109/141‑1 (MQ=255)
                                                             catcatTTGTAACATCGTCAATTTTGATATTGTGATGATTTAACATAACGACATCATCGATATTGAATTGGTATGAAAAACCTGCTGTTGCTGAATCTGTTAATTTGGCTCCAATATTTAAAATTAAATCGCTGTTGTCca                                                                                 <  4:247276/141‑1 (MQ=255)
                                                              atcatTTGTAACATCATCAATTTTGATATTGTGATGATTTAACATAACGACATCATCGATATTGAATTGGTATGAAAATCCTGCTGTTGCTGAATCTGTTAATTTGGCTCCAATATTTAAAATTAAATCGCTGTTGTCcac                                                                                >  1:398019/1‑141 (MQ=255)
                                                                catTTGTAACATCGTCAATTTTGATATTGTGATGATTTAACATAACGACATCATCGATATTGAATTGGTATGAAAAACCTGCTGTTGCTGAATCTGTTAATTTGGCTCCAATATTTAAAATTAAATCGCtgt                                                                                       >  3:218335/1‑132 (MQ=255)
                                                                catTTGTAACATCGTCAATTTTGATATTGTGATGATTTAACATAACGACATCATCGATATTGAATTGGTATGAAAAACCTGCTGTTGCTGAATCTGTTAATTTGGCTCCAATATTTAAAATTAAATCGCTGTTGTCCACAt                                                                              >  1:324716/1‑141 (MQ=255)
                                                                catTTGTAACATCGTCAATTTTGATATTGTGATGATTTAACATAACGACATCATCGATATTGAATTGGTATGAAAAACCTGCTGTTGCTGAATCTGTTAATTTGGCTCCAATATTTAAAATTAAATCGCTGTTGTCCACAt                                                                              <  2:901282/141‑1 (MQ=255)
                                                                   ttGTAACATCGTCAATTTTGATATTGTGATGATTTAACATAACGACATCATCGATATTGAATTGGTATGAAAAACCTGCTGTTGCTGAATCTGTTAATTTGGCTCCAATATTTAAAATTAAATCGCTGTTGTCCACATAAt                                                                           >  2:211432/1‑141 (MQ=255)
                                                                   ttGTAACATCGTCAATTCTGATATTGTGATGATTTAACATAACGACATCATCGATATTGAATTGGTATGAAAAACCTGCTGTTGCTGAATCTGTTAATTTGGCTCCAATATTTAAAATTAAATCGCTGTTGTCCACATAAt                                                                           >  4:152166/1‑141 (MQ=255)
                                                                     gTAACATCGTCAATTTTGATATTGTGATGATTTAACATAACGACATCATCGATATTGAATTGGTATGAAAAACCTGCTGTTGCTGAATCTGTTAATTTGGCTCCAATATTTAAAATTAAATCGCTGTTGTCCACATAAtct                                                                         <  3:72493/141‑1 (MQ=255)
                                                                     gTAACATCGTCAATTTTGATATTGTGATGATTTAACATAACGACATCATCGATATTGAATTGGTATGAAAAACCTGCTGTTGCTGAATCTGTTAATTTGGCTCCAATATTTAAAATTAAATCGCTGTTGTCCACATAAtct                                                                         >  1:55037/1‑141 (MQ=255)
                                                                       aaCATCGTCAATTTTGATATTGTGATGATTTAACATAACGACATCATCGATATTGAATTGGTATGAAAAACCTGCTGTTGCTGAATCTGTTAATTTGGCTCCAATATTTAAAATTAAATCGCTGTTGTCCACATAATCTCg                                                                       >  2:187442/1‑141 (MQ=255)
                                                                         cATCGTCAATTTTGATATTGTGATGATTTAACATAACGACATCATCGATATTGAATTGGTATGAAAAACCTGCTGTTGCTGAATCTGTTAATTTGGCTCCAATATTTAAAATTAAATCGCTGTTGTCCACATAATCTCGTa                                                                     <  1:255917/141‑1 (MQ=255)
                                                                         cATCGTCAATTTTGATATTGTGATGATTTAACATAACGACATCATCGATATTGAATTGGTATGAAAAACCTGCTGTTGCTGAATCTGTTAATTTGGCTCCAATATTTAAAATTAAATCGCTGTTGTCCACATAATCTCGTa                                                                     <  1:630108/141‑1 (MQ=255)
                                                                          aTCGTCAATTTTGATATTGTGATGATTTAACATAACGACATCATCGATATTGAATTGGTATGAAAAACCTGCTGTTGCTGAATCTGTTAATTTGGCTCCAATATTTAAAATTAAATCGCTGTTGTCCACATAATCTCGTAt                                                                    >  2:853329/1‑141 (MQ=255)
                                                                           tCGTCAATTTTGATATTGTGATGATTTAACATAACGACATCATCGATATTGAATTGGTATGAAAAACCTGCTGTTGCTGAATCTGTTAATTTGGCTCCAATATTTAAAATTAAATCGCTGTTGTCCACATAATCTCGTAtt                                                                   <  4:339619/141‑1 (MQ=255)
                                                                           tCGTCAATTTTGATATTGTGATGATTTAACATAACGACATCATCGATATTGAATTGGTATGAAAAACCTGCTGTTGCTGAATCTCTTAATTTGGCTCCAATATTTAAAATTAAATCGCTGTTGTCCACATAATCTCGTAtt                                                                   <  2:1001603/141‑1 (MQ=255)
                                                                              tCAATTTTGATATTGTGATGATTTAACATAACGACATCATCGATATTGAATTGGTATGAAAAACCTGCTGTTGCTGAATCTGTTAATTTGGCTCCAATATTTAAAATTAAATCGCTGTTGTCCACATAATCTCGTATTTTa                                                                <  1:615603/141‑1 (MQ=255)
                                                                               cAATTTTGATATTGTGATGATTTAACATAACGACATCATCGATATTGAATTGGTATGAAAAACCTGCTGTTGCTGAATCTGTTAATTTGGCTCCAATATTTAAAATTAAATCGCTGTTGTCCACATAATCTCGTATTTTAt                                                               <  2:94607/141‑1 (MQ=255)
                                                                                   tttGATATTGTGATGATTTAACATAACGACATCATCGATATTGAATTGGTATGAAAAACCTGCTGTTGCTGAATCTGTTAATTTGGCTCCAATATTTAAAATTAAATCGCTGTTGTCCACATAATCTCGTATTTTATCTTc                                                           <  1:502787/141‑1 (MQ=255)
                                                                                   tttGATATTGTGATGATTTAACATAACGACATCATCGATATTGAATTGGTATGAAAAACCTGCTGTTGCTGAATCTGTTAATTTGGCTCCAATATTTAAAATTAAATCGCTGTTGTCCACATAATCTCGTATTTTATCTTc                                                           <  3:391216/141‑1 (MQ=255)
                                                                                   tttGATATTGTGATGATTTAACATAACGACATCATCGATATTGAATTGGTATGAAAAACCTGCTGTTGCTGAATCTGTTAATTTGGCTCCAATATTTAAAATTAAATCGCTGTTGTCCACATAATCTCGTATTTTATCTTc                                                           >  2:445856/1‑141 (MQ=255)
                                                                                    ttGATATTGTGATGATTTAACATAACGACATCATCGATATTGAATTGGTATGAAAAACCTGCTGTTGCTGAATCTGTTAATTTGGCTCCAATATTTAAAATTAAATCGCTGTTGTCCACATAATCTCGTATTTTATCTTCg                                                          <  1:207308/141‑1 (MQ=255)
                                                                                      gATATTGGGATGATTTAACATAACGACATCATCGTTATTGAATTGGTATGAAAAACCTGCTGTTGCTGAATCTGTTAATTTGGCTCCAATATTTAAAATTAAATCGCTGTTGTCCACATAATCTCGTATTTTATCTTCGGc                                                        <  2:517141/141‑1 (MQ=255)
                                                                                       atatTGTGATGATTTAACATAACGACATCATCGATATTGAATTGGTATGAAAAACCTGCTGTTGCTGAATCTGTTAATTTGGCTCCAATATTTAAAATTAAATCGCTGTTGTCCACATAATCTCGTATTTTATCTTCGGCa                                                       >  1:577320/1‑141 (MQ=255)
                                                                                        tatTGTGATGATTTAACATAACGACATCATCGATATTGAATTGGTATGAAAAACCTGCTGTTGCTGAATCTGTTAATTTGGCTCCAATATTTAAAATTAAATCGCTGTTGTCCACATAATCTCGTATTTTATCTTCGGCaa                                                      <  1:427530/141‑1 (MQ=255)
                                                                                              gatgatTTAACATAACGACATCATCGATATTGAATTGGTATGAAAAACCTGCTGTTGCTGAATCTGTTAATTTGGCTCCAATATTTAAAATTAAATCGCTGTTGTCCACATAATCTCGTATTTTATCTTCGGCAATTTTcc                                                <  2:800554/141‑1 (MQ=255)
                                                                                              gatgatTTAACATAACGACATCATCGATATTGAATTGGTATGAAAAACCTGCTGTTGCTGAATCTGTTAATTTGGCTCCAATATTTAAAATTAAATCGCTGTTGTCCACATAATCTCGTATTTTATCTTCGGCAATTTTcc                                                <  1:691579/141‑1 (MQ=255)
                                                                                              gatgatTTAACATAACGACATCATCGATATTGAATTGGTATGAAAAACCTGCTGTTGCTGAATCTGTTAATTTGGCTCCAATATTTAAAATTAAATCGCTGTTGTCCACATAATCTCGTATTTTATCTTCGGCAATTTTcc                                                >  3:265333/1‑141 (MQ=255)
                                                                                                  atTTAACATAACGACATCATCGATATTGAATTGGTATGAAAAACCTGCTGTTGCTGAATCTGTTAATTTGGCTCCAATATTTAAAATTAAATCGCTGTTGTCCACATAATCTCGTATTTTATCTTCGGCAATTTTCTCATc                                            >  1:772347/1‑141 (MQ=255)
                                                                                                  atTTAACATAACGACATCATCGATATTGAATTGGTATGAAAAACCTGCTGTTGCTGAATCTGTTAATTTGGCTCCAATATTTAAAATTAAATCGCTGTTGTCCACATAATCTCGTATTTTATCTTCGGCAATTTTCCCATc                                            <  2:836770/141‑1 (MQ=255)
                                                                                                  atTTAACATAACGACATCATCGATATTGAATTGGTATGAAAAACCTGCTGTTGCTGAATCTGTTAATTTGGCTCCAATATTTAAAATTAAATCGCTGTTGTCCACATAATCTCGTATTTTATCTTCGGCAATTTTCCCATc                                            >  3:142948/1‑141 (MQ=255)
                                                                                                      aaCATAACGACATCATCGATATTGAATTGGTATGAAAAACCTGCTGTTGCTGAATCTGTTAATTTGGCTCCAATATTTAAAATTAAATCGCTGTTGTCCACATAATCTCGTATTTTATCTTCGGCAATTTTCCCATCGTaa                                        >  4:192210/1‑141 (MQ=255)
                                                                                                      aaCATAACGACATCATCGATATTGAATTGGTATGAAAAACCTGCTGTTGCTGAATCTGTTAATTTGGCTCCAATATTTAAAATTAAATCGCTGTTGTCCACATAATCTCGTATTTTATCTTCGGCAATTTTCCCATCGTaa                                        >  4:274255/1‑141 (MQ=255)
                                                                                                      aaCATAACGACATCATCGATATTGAATTGGTATGAAAAACCTGCTGTTGCTGAATCTGTTAATTTGGCTCCAATATTTAAAATTAAATCGCTGTTGTCCACATAATCTCGTATTTTATCTTCGGCAATTTTCCCATCGTaa                                        >  1:529624/1‑141 (MQ=255)
                                                                                                      aaCATAACGACATCATCGATATTGAATTGGTATGAAAAACCTGCTGTTGCTGAATCTGTTAATTTGGCTCCAATATTTAAAATTAAATCGCTGTTGTCCACATAATCTCGTATTTTATCTTCGGCAATTTTCCCATCGTaa                                        >  2:505505/1‑141 (MQ=255)
                                                                                                       aCATAACGACATCATCGATATTGAATTGGTATGAAAAACCTGCTGTTGCTGAATCTGTTAATTTGGCTCCAATATTTAAAATTAAATCGCTGTTGTCCACATAATCTCGTATTTTATCTTCGGCAATTTTCCCATCGTaaa                                       >  2:837103/1‑141 (MQ=255)
                                                                                                         aTAACGACATCATCGATATTGAATTGGTATGAAAAACCTGCTGTTGCTGAATCTGTTAATTTGGCTCCAATATTTAAAATTAAATCGCTGTTGTCCACATAATCTCGTATTTTATCTTCGGCAATTTTCCCATCGTAAATa                                     >  4:284630/1‑141 (MQ=255)
                                                                                                            aCGACATCATCGATATTGAATTGGTATGAAAAACCTGCTGTTGCTGAATCTGTTAATTTGGCTCCAATATTTAAAATTAAATCGCTGTTGTCCACATAATCTCGTATTTTATCTTCGGCAATTTTCCCATCGTAAATAccc                                  >  1:678268/1‑141 (MQ=255)
                                                                                                             cGACATCATCGATATTGAATTGGTATGAAAAACCTGCTGTTGCTGAATCTGTTAATTTGGCTCCAATATTTAAAATTAAATCGCTGTTGTCCACATAATCTCGTATTTTATCTTCGGCAATTTTCCCATCGTAAATACCCa                                 <  1:146098/141‑1 (MQ=255)
                                                                                                             cGACATCATCGATATTGAATTGGTATGAAAAACCTGCTGTTGCTGAATCTGTTAATTTGGCTCCAATATTTAAAATTAAATCGCTGTTGTCCACATAATCTCGTATTTTATCTTCGGCAATTTTCCCATCGTAAATACCCa                                 >  1:75408/1‑141 (MQ=255)
                                                                                                               aCATCATCGATATTGAATTGGTATGAAAAACCTGCTGTTGCTGAATCTGTTAATTTGGCTCCAATATTTAAAATTAAATCGCTGTTGTCCACATAATCTCGTATTTTATCTTCGGCAAGTTTCCCATCGTAAATACCCatt                               >  2:801808/1‑140 (MQ=255)
                                                                                                                catcatCGATATTGAATTGGTATGAAAAACCTGCTGTTGCTGAATCTGTTAATTTGGCTCCAATATTTAAAATTAAATCGCTGTTGTCCACATAATCTCGTATTTTATCTTCGACAATTTTCCCATCGTAAATACCCatat                              >  1:1042197/1‑141 (MQ=255)
                                                                                                                    atcGATATTGAATTGGTATGAAAATCCTGCTGTTGCTGAATCTGTTAATTTGGCTCCAATATTTAAAATTAAATCGCTGTTGTCCACATAATCTCGTATTTTATCTTCGGCAATTTTCCCGTCGTAAATACCCATataata                          <  3:59455/141‑1 (MQ=255)
                                                                                                                       gATATTGAATTGGTATGAAAAACCTGCTGTTGCTGAATCTGTTAATTTGGCTCCAATATTTAAAATTAAATCGCTGTTGTCCACATAATCTCGTATTTTATCTTCGGCAATTTTCCCATCGTAAATACCCATATAATATgg                       >  1:1061825/1‑141 (MQ=255)
                                                                                                                         tatTGAATTGGTATGAAAAACCTGCTGTTGCTGAATCTGTTAATTTGGCTCCAATATTTAAAATTAAATCGCTGTTGTCCACATAATCTCGTATTTTATCTTCGGCAATTTTCCCATCGTAAATACCCATATAATATGGAt                     <  1:156707/141‑1 (MQ=255)
                                                                                                                             gAATTGGTATGAAAAACCTGCTGTTGCTGAATCTGTTAATTTGGCTCCAATATTTAAAATTAAATCGCTGTTGTCCACATAATCTCGTATTTTATCTTCGGCAATTGTCCCATCGTAAATACCCATATAATATGGATTTTc                 >  1:1007349/1‑141 (MQ=255)
                                                                                                                             gAATTGGCATGAAAAACCTGCTGTTGCTGAATCTGTTAATTTGGCTCCAATATTTAAAATTAAATCGCTGTTGTCCACATAATCTCGTATTTTATCTTCGGCAATTTTCCCATCGTAAATACCCATATAATATGGATTTTc                 >  2:1085687/1‑141 (MQ=255)
                                                                                                                              aaTTGGTATGAAAAACCTGCTGTTGCTGAATCTGTTAATTTGGCTCCAATATTTAAAATTAAATCGCTGTTGTCCACATAATCTCGTATTTTATCTTCGGCAATTTTCCCATCGTAAATACCCATATAATATGGATTTTcc                <  4:9770/141‑1 (MQ=255)
                                                                                                                               aTTGGTATGAAAAACCTGCTGTTGCTGAATCTGTTAATTTGGCTCCAATATTTAAAATTAAATCGCTGTTGTCCACATAATCTCGTATTTTATCTTCGGCAATTTTCCCATCGTAAATACCCATATAATATGGATTTTCCt               <  2:608442/141‑1 (MQ=255)
                                                                                                                               aTTGGTATGAAAAACCTGCTGTTGCTGAATCTGTTAATTTGGCTCCAATATTTAAAATTAAATCGCTGTTGTCCACATAATCTCGTATTTTATCTTCGGCAATTTTCCCATCGTAAATACCCATATAATATGGATTTTCCt               <  4:412507/141‑1 (MQ=255)
                                                                                                                                  ggTATGAAAAACCTGCTGTTGCTGAATCTGTTAATTTGGCTCCAATATTTAAAATTAAATCGCTGTTGTCCACATAATCTCGTATTTTATCTTCGGCAATTTTCCCATCGTAAATACCCATATAATATGGATTTTCCTCAt            <  4:143561/141‑1 (MQ=255)
                                                                                                                                         aaaaCCTGCTGTTGCTGAATCTGTTAATTTGGCTCCAATATTTAAAATTAAATCGCTGTTGTCCACATAATCTCGTATTTTATCTTCGGCAATTTTCCCATCGTAAATACCCATATAATATGGATTTTCCTCATTAAAAGc     >  4:137420/1‑141 (MQ=255)
                                                                                                                                            aCCTGCTGTTGCTGAATCTGTTAATTTGGCTCCAATATTTAAAATTAAATCGCTGTTGTCCACATAATCTCGTATTTTATCTTCGGCAATTTTCCCATCGTAAATACCCATATAATATGGATTTTCCTCATTAAAAGCAcc  >  1:653923/1‑141 (MQ=255)
                                                                                                                                            aCCTGCTGTTGCTGAATCTGTTAATTTGGCTCCAATATTTAAAATTAAATCGCTGTTGTCCACATAATCTCGTATTTTATCTTCGGCAATTTTCCCATCGTAAATACCCATATAATATGGATTTTCCTCATTAAAAGCAcc  >  2:421910/1‑141 (MQ=255)
                                                                                                                                            aCCTGCTGTTGCTGAATCTGTTAATTTGGCTCCAATATTTAAAATTAAATCGCTGTTGTCCACATAATCTCGTATTTTATCTTCGGCAATTTTCCCATCGTAAATACCCATATAATATGGAGTTTCCTCATTAAAAGCAcc  >  1:526295/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                            
GTTGCGTTATTCGTATATGAAATATTGGATAACTGTTTTAACAATGATGGTAGAGATATTTCATCATTTGTAACATCGTCAATTTTGATATTGTGATGATTTAACATAACGACATCATCGATATTGAATTGGTATGAAAAACCTGCTGTTGCTGAATCTGTTAATTTGGCTCCAATATTTAAAATTAAATCGCTGTTGTCCACATAATCTCGTATTTTATCTTCGGCAATTTTCCCATCGTAAATACCCATATAATATGGATTTTCCTCATTAAAAGCACC  >  USA300TCH1516_ALE/219948‑220228

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: