Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 225,063 T→A 5.3% I50I (ATT→ATA)  murQ → N‑acetylmuramic acid 6‑phosphate etherase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE225,0630TA 5.3% 414.9 / 6.6 134I50I (ATT→ATA) murQN‑acetylmuramic acid 6‑phosphate etherase
Reads supporting (aligned to +/- strand):  ref base T (67/58);  new base A (4/5);  total (71/63)
Fisher's exact test for biased strand distribution p-value = 7.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 142 total aligned reads displayed.

AGTACGACCGAAGCGCGTAATGAAGCGACGATGCATCTTGATGAAATGACTGTGGAAGAGGCTTTAATTACGATGAATAAAGAAGATCAGCAAGTCCCGTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTTATATCGGTGCAGGTACAAGTGGAAGGTTGGGTGTCTTAGATGCAGCGGAGTGTGTACCTACATTCAATACTGACCCTCATGAAATTATAGGTATTAT  >  USA300TCH1516_ALE/224923‑225203
                                                                                                                                            |                                                                                                                                            
aGTACGACCGAAGCGCGTAATGAAGCGACGATGCATCTTGATGAAATGACTGTGGAAGAGGCTTTAATTACGATGAATAAAGAAGATCAGCAAGTCCCGTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAAtt                                                                                                                                              >  1:707301/1‑141 (MQ=255)
aGTACGACCGAAGCGCGTAATGAAGCGACGATGCATCTTGATGAAATGACTGTGGAAGAGGCTTTAATTACGATGAATAAAGAAGATCAGCAAGTCCCGTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAAtt                                                                                                                                              <  1:7407/141‑1 (MQ=255)
   aCGTCCGAAGCGCGTAATGAAGCGACGATGCATCTTGATGAAATGACTGTCGAAGAGGCTTTAATTACGATGAATAAAGAAGATCAGCAAGTCCCGTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAATTaaa                                                                                                                                           <  2:784157/141‑1 (MQ=255)
    cGACCGAAGCGCGTAATGAAGCGACGATGCATCTTGATGAAATGACTGTGGAAGAGGCTTCAATTACGATGAATAAAGAAGATCAGCAAGTCCCGTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAATTaaaa                                                                                                                                          <  1:733270/141‑1 (MQ=255)
        cGAAGCGCGTAATGAAGCGACGATGCATCTTGATGAAATGACTGTGGAAGAGGCTTTAATTACGATGAATAAAGAAGATCAGCAAGTCCCGTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAAc                                                                                                                                      <  1:797620/141‑1 (MQ=255)
           aGCGCGTAATGAAGCGACGATGCATCTTGATGAAATGACTGTGGAAGAGGCTTTAATTACGATGAATAAAGAAGATCAGCAAGTCCCGTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAAt                                                                                                                                   >  3:250450/1‑141 (MQ=255)
           aGCGCGTAATGAAGCGACGATGCATCTTGATGAAATGACTGTGGAAGAGGCTTTAATTACGATGAATAAAGAAGATCAGCAAGTCCCGTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAAt                                                                                                                                   <  4:295148/141‑1 (MQ=255)
                     gAAGCGACGATGCATCTTGATGAAATGACTGTGGAAGAGGCTTTAATTACGATGAATAAAGAAGATCAGCAAGTCCCGTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGtat                                                                                                                         <  1:997326/141‑1 (MQ=255)
                     gAAGCGACGATGCATCTTGATGAAATGACTGTGGAAGAGGCTTTAATTACGATGAATAAAGAAGATCAGCAAGTCCCGTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGtat                                                                                                                         >  1:104705/1‑141 (MQ=255)
                     gAAGCGACGATGCATATTGATGAAATGACTGTGGAAGAGGCTTTAATTACGATGAATAAAGAAGATCAGCAAGTCCCGTTAGCAGTTCGAAAGGCAATAACACAATTGACAAAAGTCATTAACACAAAAATTGAACAGtat                                                                                                                         >  4:223177/1‑141 (MQ=255)
                        gCGACGATGCAGCTTGATGAAATGACTGTGGAAGAGGCTTTAATTACGATGAATAAAGAAGATCAGCAAGTCCCGTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATaaa                                                                                                                      <  2:208618/141‑1 (MQ=255)
                           acgaTGCATCTTGATGAAATGACTGTGGAAGAGGATTTAATTACGATGAATAAAGAAGATCAGCAAGACTCGTTAGCAGTGCGAAAGGCAATACCACAATTGACAAAAGTAATAAAAAAAACAATTACAAAGTATAAAAAg                                                                                                                   >  4:32826/1‑141 (MQ=255)
                             gaTGCATCTTGATGAAATGACTGTGGAAGAGGCTTTAATTACGATGAATAAAGAAGATCAGCAAGTCCCGTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAggg                                                                                                                 <  4:156210/141‑1 (MQ=255)
                               tGCATCTTGATGAAATGACTGTGGAAGAGGCTTTAATTACGATGAATAAAGAAGATCAGCAAGTCCCGTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTg                                                                                                               <  4:409885/141‑1 (MQ=255)
                               tGCATCTTGATGAAATGACTGTGGAAGAGGCTTTAATTACGATGAATAAAGAAGATCAGCAAGTCCCGTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTg                                                                                                               <  4:435398/141‑1 (MQ=255)
                               tGCATCTTGATGAAATGACTGTGGAAGAGGCTTTAATTACGATGAATAAAGAAGATCAGCAAGTCCCGTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTg                                                                                                               <  4:201724/141‑1 (MQ=255)
                                gCATCTTGATGAAATGACTGTGGAAGAGGCTTTAATTACGATGAATAAAGAAGATCAGCAAGTCCCGTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTgg                                                                                                              <  1:294539/141‑1 (MQ=255)
                                gCATCTTGATGAAATGACTGTGGAAGAGGCTTTAATTACGATGAATAAAGAAGATCAGCAAGTCCCGTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAATAAAAAAAACAATTGCACAGTATAAAAAGGGTgg                                                                                                              <  1:56668/141‑1 (MQ=255)
                                gCATCTTGATGAAAGGACTGTGGAAGAGGCTTAAAATACGATAAATAAAGAAGATCAACAAGCCCAGTAAGAAGTTCAAAAGGCAAAGCCACAAAAAACAAAAGTAAATAAAAAAACAATTGCACAGTATAAAAAGGGTgg                                                                                                              <  1:548917/141‑1 (MQ=255)
                                 cATCTTGATGAAATGACTGTGGAAGAGGCTTTAATTACGATGAATAAAGAAGATCAGCAAGTCCCGTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGa                                                                                                             <  3:428558/141‑1 (MQ=255)
                                   tCTTGATGAAATGACTGTGGAAGAGGCTTTAATTACGATGAATAAAGAAGATCAGCAAGTCCCGTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACg                                                                                                           >  1:1013100/1‑141 (MQ=255)
                                       gatgaAATGACTGTGGAAGAGGCTTTAATTACGATGAATAAAGAAGATCAGCAAGTCCCGTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACgattg                                                                                                       <  3:233557/141‑1 (MQ=255)
                                         tgaAATGACTGTGGAAGAGGCTTTGATGACGATGAATAAAGAAGATCAGCAAGTCCCGTTAGCAGTTCGAAAGGCAATACCACAATTGAAAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACgattgat                                                                                                     <  2:438050/141‑1 (MQ=255)
                                          gaAATGACTGTGGAAGAGGCTTTAATTAAGATGAATAAAAAATATCAGCAAGTCCCGTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACgattgatt                                                                                                    <  4:383426/141‑1 (MQ=255)
                                            aaTGACTGTGGAAGAGGCTTTAATTACGATGAATAAAGAAGATCAGCAAGTCCCGTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTta                                                                                                  <  1:543077/141‑1 (MQ=255)
                                            aaTGACTGTGGAAGAGGCTTTAATTACGATGAATAAAGAAGATCAGCAAGTCCCGTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTta                                                                                                  >  3:313116/1‑141 (MQ=255)
                                              tGACTGTGGAAGAGGCTTTAATTACAAAGAATAAAGAAGATCAGCAAGTCCCGTTAGCAATTCAAAAGGCAATACCACAATTGACAAAAAAAAAAAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTtata                                                                                                <  2:692907/141‑1 (MQ=255)
                                               gACTGTGGAAGAGGCTTTAATTACGATGAATAAAGAAGATCAGCAAGTCCCGTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTTatat                                                                                               >  4:419235/1‑141 (MQ=255)
                                                aCTGTGGAAGAGGCTTTAATTACGATGAATAAAGAAGATCAGCAAGTCCCGTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTTATATc                                                                                              >  1:285055/1‑141 (MQ=255)
                                                aCTGTGGAAGAGGCTTTAATTACGATGAATAAAGAAGATCAGCAAGTCCCGTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTTATATc                                                                                              >  2:73537/1‑141 (MQ=255)
                                                 cTGTGGAAGAGGCTTTAATTACGATGAATAAAGAAGATCAGCAAGTCCCGTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTTATATCg                                                                                             >  4:285014/1‑141 (MQ=255)
                                                  tgtgGAAGAGGCTTTAATTACGATGAATAAAGAAGATCAGCAAGTCCCGTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAATAAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATCTATATCgg                                                                                            >  2:787803/1‑141 (MQ=255)
                                                  tgtgGAAGAGGCTTTAATTACGATGAATAAAGAAGATCAGCAAGTCCCGTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAATAAAAAAAACAATTGCACAGGATAAAAAGGGTGGACGTTTGATCTATAACgg                                                                                            >  1:707079/1‑141 (MQ=255)
                                                   gtgGAAGAGGCTTTAATTACGATGAATAAAGAAGATCAGCAAGTCCCGTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTTATATAGGt                                                                                           >  1:546279/1‑141 (MQ=255)
                                                     ggAAGAGGCTTTAATTACGATGAATAAAGAAGATCAGCAAGTCCCGTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTTATATCGGTGc                                                                                         >  1:258545/1‑141 (MQ=255)
                                                        agagGCTTTAATTACGATGAATAAAGAAGATCAGCAAGTCCCGTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTTATATCGGTGCAgg                                                                                      >  1:932038/1‑141 (MQ=255)
                                                          agGCTTTAATTACGATGAATAAAGAAGATCAGCAAGTCCCGTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTTATATCGGTGCAGGTa                                                                                    <  1:478777/141‑1 (MQ=255)
                                                          agGCTTTAATTACGATGAATAAAGAAGATCAGCAAGTCCCGTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAAAAAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTTATATCGGTGCAGGTa                                                                                    <  2:447886/141‑1 (MQ=255)
                                                           ggCTTTAATTACGATGAATAAAGAAGATCAGCAAGTCCCGTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAATACAAGTGCACAGTATAAAAAGGGTGGACGATTGATTTATATCGGTGCAGGTAc                                                                                   >  1:486694/1‑141 (MQ=255)
                                                           ggCTTTAATTACGATGAATAAAGAAGATCAGCAAGTCCCGTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTTATATCGGTGCAGGTAc                                                                                   >  1:1008986/1‑141 (MQ=255)
                                                           ggCTGTAATTACGATGAATATAGAAGATCAGCAAGTCCTGGTAGCAGTTCGAAAGGCAATATCCCAATTAACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAAGGAGGACCATTAACTTATATCGGCACAGATAc                                                                                   >  4:235476/1‑141 (MQ=255)
                                                              tttAATTACGATGAATACAGAAGATCAGCAAGTCCCGTTAGCAGTTCGAAAGGCAATACCCCCATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTTATATCGGTGCAGGTACAAg                                                                                <  1:503449/141‑1 (MQ=255)
                                                                tAATTACGATGAATAAAGAAGATCAGCAAGTCCCGTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTTATATCGGTGCAGGTACAAGTg                                                                              >  2:399253/1‑141 (MQ=255)
                                                                tAATTACGATGAATAAAGAAGATCAGCAAGTCCCGTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTTATATCGGTGCAGGTACAAGTg                                                                              <  1:189993/141‑1 (MQ=255)
                                                                 aaTTACGATGAATAAAGAAGATCAGCAAGTCCCGTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTTATATCGGTGCAGGTACAAGTgg                                                                             <  1:174908/141‑1 (MQ=255)
                                                                     aCGATGAATAAAGAAGATCAGCAAGTCCCGTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTTATATCGGTGCAGGTACAAGTGGAAgg                                                                         >  2:153072/1‑141 (MQ=255)
                                                                        aTGAATAAAGAAGATCAGCAAGTCCCGTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTTATATCGGTGCAGGTACAAGTGGAAGGTTg                                                                      >  2:412861/1‑141 (MQ=255)
                                                                        aTGAATAAAGAAGATCAGCAAGTCCCGTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTTATATCGGTGCAGGTACAAGTGGAAGGTTg                                                                      <  2:528247/141‑1 (MQ=255)
                                                                        aTGAATAAAGAAGATCAGCAAGTCCCGTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTTATATCGGTGCAGGTACAAGTGGAAGGTTg                                                                      <  2:30660/141‑1 (MQ=255)
                                                                         tGAATAAAGAAGATCAGCAAGTCCCGTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTTATATCGGTGCAGGTACAAGTGGAAGGTTgg                                                                     <  1:168026/141‑1 (MQ=255)
                                                                         tGAATAAAGAAGATAAGCAAGTACCGTTAGCAGTTCTAAAGGCAATACCACAATTGACAAAAGAAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTTATATCGGTGCAGGTACAAGTGGAAGGTTgg                                                                     <  4:101196/141‑1 (MQ=255)
                                                                          gAATAAAGAAGATCAGCAAGTCCCGTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTTATATCGGTGCAGGTACAAGTGGAAGGTTggg                                                                    <  1:1012009/141‑1 (MQ=255)
                                                                          gAATAAAGAAGATCAGCAAGTCCCGTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTTATATCGGTGCAGGTACAAGTGCAAGGTTggg                                                                    <  1:952667/141‑1 (MQ=255)
                                                                           aaTAAAGAAGATCAGCAAGTCCCGTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGAAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTTATATCGGTGCAGGTACAAGTGGAAGGTTGGgt                                                                   <  2:577671/141‑1 (MQ=255)
                                                                               aagaagATCAGCAAGTCCCGTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTTATATCGGTGCAGGTACAAGTGGAAGGTTGGGTGTCt                                                               >  1:311766/1‑141 (MQ=255)
                                                                                agaagaTCAGCAAGTCCCGTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTTATATCGGTGCAGGTACAAGTGGAAGGTTGGGTGTCtt                                                              >  3:204600/1‑141 (MQ=255)
                                                                                agaagaTCAGCAAGTCCCGTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTTATATCGGTGCAGGTACAAGTGGAAGGTTGGGTGTCtt                                                              >  2:290220/1‑141 (MQ=255)
                                                                                  aagaTCAGCAAGTCCCGTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTTATATCGGTGCAGGTACAAGTGGAAGGTTGGGTGTCTTAg                                                            >  2:610614/1‑141 (MQ=255)
                                                                                    gaTCAGCAAGTCCCGTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTTATATCGGTGCAGGTACAAGTGGAAGGTTGGGTGTCTTAGAt                                                          >  2:1031094/1‑141 (MQ=255)
                                                                                      tCAGCAAGTCCCGTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTTATATCGGTGCAGGTACAAGCGGAAGGTTGGGTGTCTTAGATGc                                                        >  1:114525/1‑141 (MQ=255)
                                                                                        aGCAAGTCCCGTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTTATATCGGTGCAGGTACAAGTGGAAGGTTGGGTGTCTTAGATGCAg                                                      <  2:128421/141‑1 (MQ=255)
                                                                                         gCAAGTCCCGTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATaaaa                                                                                                                     <  2:345374/77‑1 (MQ=255)
                                                                                         gCAAGTCCCGTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATaaaa                                                                                                                     >  1:345374/1‑77 (MQ=255)
                                                                                         gCAAGTCCCGTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTTATATCGGTGCAGGTACAAGTGGAAGGTTGGGTGTCTTAGATGCAGc                                                     >  4:140488/1‑141 (MQ=255)
                                                                                         gCAAGTCCCGTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTTATATCGGTGCAGGTACAAGTGGAAGGTTGGGTGTCTTAGATGCAGc                                                     >  4:34853/1‑141 (MQ=255)
                                                                                          cAAGTCCCGTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTTATATCGGTGCAGGTACAAGTGGAAGGTTGGGTGTCTTAGATGCAGCg                                                    >  2:923792/1‑141 (MQ=255)
                                                                                           aaGTCCCGTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTTATATCGGTGCAGGTACAAGTGGAAGGTTGGGTGTCTTAGATGCAGCgg                                                   >  3:202862/1‑141 (MQ=255)
                                                                                                ccGTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTTATATCGGTGCAGGTACAAGTGGAAGGTTGGGTGTCTTAGATGCAGCGGAgtgt                                              >  1:66591/1‑141 (MQ=255)
                                                                                                  gTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTTATATCGGTGCAGGTACAAGTGGAAGGTTGGGTGTCTTAGATGCAGCGGAgtgtgt                                            >  2:742999/1‑141 (MQ=255)
                                                                                                  gTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTTATATCGGTGCAGGTACAAGTGGAAGGTTGGGTGTCTTAGATGCAGCGGAgtgtgt                                            >  1:277164/1‑141 (MQ=255)
                                                                                                   ttAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTTATATCGGTGCAGGTACAAGTGGAAGGTTGGGTGTCTTAGATGCAGCGGAGTGTGTa                                           >  3:337/1‑141 (MQ=255)
                                                                                                   ttAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTTATATCGGTGCAGGTACAAGTGGAAGGTTGGGTGTCTTAGATGCAGCGGAGTGTGTa                                           >  2:452907/1‑141 (MQ=255)
                                                                                                   ttAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTTATATCGGTGCAGGTACAAGTGGAAGGTTGGGTGTCTTAGATGCAGCGGAGTGTGTa                                           >  2:882972/1‑141 (MQ=255)
                                                                                                       cAGTTCGAAAGGCAATACCACACTTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTTATATCGGTGCAGGTACAAGTGGAAGGTTGGGTGTCTTAGATGCAGCGGAGTGTGTACCTa                                       <  2:1051784/141‑1 (MQ=255)
                                                                                                          ttCGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTTATATCGGTGCAGGTACAAGTGGAAGGTTGGGTGTCTTAGATGCAGCGGAGTGTGTACCTACAt                                    >  4:42033/1‑141 (MQ=255)
                                                                                                           tCGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTTATATCGGTGCAGGTACAAGTGGAAGGTTGGGTGTCTTAGATGCAGCGGAGTGTGTACCTACAtt                                   <  2:231440/141‑1 (MQ=255)
                                                                                                            cGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTTATATCGGTGCAGGTACAAGTGGAAGGTTGGGTGTCTTAGATGCAGCGGAGTGTGTACCTACATTc                                  <  2:17247/141‑1 (MQ=255)
                                                                                                                aGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTTATATCGGTGCAGGTACAAGTGGAAGGTTGGGTGTCTTAGATGCAGCGGAGTGTGTACCTACATTCAATa                              >  3:21569/1‑141 (MQ=255)
                                                                                                                aGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTTATATCGGTGCAGGTACAAGTGGAAGGTTGGGTGTCTTAGATGCAGCGGAGTGTGTACCTACATTCAATa                              <  1:924366/141‑1 (MQ=255)
                                                                                                                aGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTTATATCGGTGCAGGTACAAGTGGAAGGTTGGGTGTCTTAGATGCAGCGGAGTGTGTACCTACATTCAATa                              <  1:405998/141‑1 (MQ=255)
                                                                                                                 ggCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTTATATCGGTGCAGGTACAAGTGGAAGGTTGGGTGTCTTAGATGCAGCGGAGTGTGTACCTACATTCAATAc                             >  2:114133/1‑141 (MQ=255)
                                                                                                                 ggCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTTATATCGGTGCAGGTACAAGTGGAAGGTTGGGTGTCTTAGATGCAGCGGAGTGTGTACCTACATTCAATAc                             >  1:438668/1‑141 (MQ=255)
                                                                                                                      tACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTTATATCGGTGCAGGTACAAGTGGAAGGTTGGGTGTCTTAGATGCAGCGGAGTGTGTACCTACATTCAATACTGAcc                        <  2:929207/141‑1 (MQ=255)
                                                                                                                      tACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTTATATCGGTGCAGGTACAAGTGGAAGGTTGGGTGTCTTAGATGCAGCGGAGTGTGTACCTACATTCAATACTGAcc                        >  3:83985/1‑141 (MQ=255)
                                                                                                                      tACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTTATATCGGTGCAGGTACAAGTGGAAGGTTGGGTGTCTTAGATGCAGCGGAGTGTGTACCTACATTCAATACTGAcc                        >  1:758194/1‑141 (MQ=255)
                                                                                                                      tACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTTATATCGGTGCAGGTACAAGTGGAAGGTTGGGTGTCTTAGATGCAGCGGAGTGTGTACCTACATTCAATACTGAcc                        <  1:349397/141‑1 (MQ=255)
                                                                                                                       aCCACAATTGAAAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTTATATCGGTGCAGGTACAAGTGGAAGGTTGGGTGTCTTAGATGCAGCGGAGTGTGTACCTACATTCAATACTGAccc                       >  1:158597/1‑141 (MQ=255)
                                                                                                                        ccACAATTGATAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTTATATCGGTGCAGGTACAAGTGGAAGGTTGGGTGTCTTAGATGCAGCGGAGTGTGTACCTACATTCAATACTGACCCt                      <  2:1077590/141‑1 (MQ=255)
                                                                                                                           caATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTTATATCGGTGCAGGTACAAGTGGAAGGTTGGGTGTCTTAGATGCAGCGGAGTGTGTACCTACATTCAATACTGACCCTCAt                   >  2:1046576/1‑141 (MQ=255)
                                                                                                                           caATTAACAAAAGTAATTAAAAAAACAAGTGCACAGTATAATAAGGGTGGACGATTGATTTATATCGGTGCAGGTACAAGTGGAAGGTTGGGTGTCTTAGATGCAGCGGAGTGTGTACCTACATTCAATACTGACCCTCAt                   <  2:979086/141‑1 (MQ=255)
                                                                                                                            aaTTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTTATATCGGTGCAGGTACAAGTGGAAGGTTGGGTGTCTTAGATGCAGCGGAGTGTGTACCTACATTCAATACTGACCCTCATg                  >  1:850987/1‑141 (MQ=255)
                                                                                                                               tGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTTATATCGGTGCAGGTACAAGTGGAAGGTTGGGTGTCTTAGATGCAGCGGAGTGTGTACCTACATTCAATACTGACCCTCATGaaa               >  4:50175/1‑141 (MQ=255)
                                                                                                                                  cAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTTATATCGGTGCAGGTACAAGTGGAAGGTTGGGTGTCTTAGATGCAGCGGAGTGTGTACCTACATTCAATACTGACCCTCATGAAATta            >  3:136159/1‑141 (MQ=255)
                                                                                                                                      aGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTTATATCGGTGCAGGTACAAGTGGAAGGTTGGGTGTCTTAGATGCAGCGGAGTGTGTACCTACATTCAATACTGACCCTCATGAAATTATAgg        <  1:12132/141‑1 (MQ=255)
                                                                                                                                         aaTTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTTATATCGGTGCAGGTACAAGTGGAAGGTTGGGTGTCTTAGATGCAGCGGAGTGTGTACCTACATTCAATACTGACCCTCATGAAATTATAGGtat     >  3:10940/1‑141 (MQ=255)
                                                                                                                                          agtAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTTATATCGGTGCAGGTACAAGTGGAAGGTTGGGTGTCTTAGATGCAGCGGAGTGTGTACCTACATTCAATACTGACCCTCATGAAATTATAGGtatt    <  2:637761/139‑1 (MQ=255)
                                                                                                                                           ttAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTTATATCGGTGCAGGTACAAGTGGAAGGTTGGGTGTCTTAGATGCAGCGGAGTGTGTACCTACATTCAATACTGACCCTCATGAAATTATAGGtatta   >  1:132378/1‑141 (MQ=255)
                                                                                                                                           ttAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTTATATCGGTGCAGGTACAAGTGGAAGGTTGGGTGTCTTAGATGCAGCGGAGTGTGTACCTACATTCAATACTGACCCTCATGAAATTATAGGtatta   <  4:39112/141‑1 (MQ=255)
                                                                                                                                            tAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTTATATCGGTGCAGGTACAAGTGGAAGGTTGGGTGTCTTAGATGCAGCGGAGTGTGTACCTACATTCAATACTGACCCTCATGAAATTATAGGtattat  >  3:192371/1‑141 (MQ=255)
                                                                                                                                            tAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTTATATCGGTGCAGGTACAAGTGGAAGGTTGGGTGTCTTAGATGCAGCGGAGTGTGTACCTACATTCAATACTGACCCTCATGAAATTATAGGtattat  >  1:1075759/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                            
AGTACGACCGAAGCGCGTAATGAAGCGACGATGCATCTTGATGAAATGACTGTGGAAGAGGCTTTAATTACGATGAATAAAGAAGATCAGCAAGTCCCGTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTTATATCGGTGCAGGTACAAGTGGAAGGTTGGGTGTCTTAGATGCAGCGGAGTGTGTACCTACATTCAATACTGACCCTCATGAAATTATAGGTATTAT  >  USA300TCH1516_ALE/224923‑225203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑