Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 238,173 T→C 5.3% I58I (ATT→ATC gsiD_2 → Glutathione transport system permease protein GsiD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE238,1730TC 5.3% 414.8 / 6.0 135I58I (ATT→ATCgsiD_2Glutathione transport system permease protein GsiD
Reads supporting (aligned to +/- strand):  ref base T (59/68);  new base C (4/4);  total (63/72)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 141 total aligned reads displayed.

AATTATTCGCAAGTTACGCCATAGCAACTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGACATTAACAGGCAACGTATTTACATTATTAATGTTTGTAAGTATTAGACGTTACCAACGTACA  >  USA300TCH1516_ALE/238034‑238311
                                                                                                                                           |                                                                                                                                          
aaTTATTCGCAAGTTACGCCATAGCAACTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAattatta                                                                                                                                           <  2:183103/141‑1 (MQ=255)
   tattCGCAAGTTACGCCATAGCAACTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATTATTTTAATTATTAttt                                                                                                                                        >  3:412664/1‑141 (MQ=255)
   tattCGCAAGTTACGCCATAGCAACTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTAttt                                                                                                                                        <  1:889128/141‑1 (MQ=255)
     ttCGCAAGTTACGCCATAGCAACTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTa                                                                                                                                      >  2:796638/1‑141 (MQ=255)
      tCGCAAGTTACGCCATAGCAACTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTaa                                                                                                                                     >  1:420265/1‑141 (MQ=255)
       cGCAAGTTACGCCATAGCAACTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTaac                                                                                                                                    >  3:441125/1‑141 (MQ=255)
            gTTACGCCATAGCAACTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACgg                                                                                                                               <  1:732109/141‑1 (MQ=255)
            gTTACGCCATAGCAACTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACgg                                                                                                                               <  3:32126/141‑1 (MQ=255)
            gTTACGCCATAGCAACTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACgg                                                                                                                               <  1:732118/141‑1 (MQ=255)
            gTTACGCCATAGCAACTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACgg                                                                                                                               <  1:343532/141‑1 (MQ=255)
              tACGCCATAGCAACTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGaa                                                                                                                             <  2:931715/141‑1 (MQ=255)
                cGCCATAGCAACTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGaaca                                                                                                                           <  2:1011595/141‑1 (MQ=255)
                cGCCATAGCAACTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGaaca                                                                                                                           <  2:565768/141‑1 (MQ=255)
                 gCCATAGCAACTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGaacaa                                                                                                                          >  1:1043509/1‑141 (MQ=255)
                 gCCATAGCAACTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGaacaa                                                                                                                          >  1:834163/1‑141 (MQ=255)
                  ccATAGCAACTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGaacaaa                                                                                                                         <  1:724572/141‑1 (MQ=255)
                       gCAACTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATgg                                                                                                                    <  4:10629/141‑1 (MQ=255)
                        cAACTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGa                                                                                                                   >  3:422277/1‑141 (MQ=255)
                          aCTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAg                                                                                                                 <  1:820751/141‑1 (MQ=255)
                           cTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGc                                                                                                                <  2:86113/141‑1 (MQ=255)
                             tataTTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGcat                                                                                                              >  1:1065936/1‑141 (MQ=255)
                              atatTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGcatc                                                                                                             >  3:19036/1‑141 (MQ=255)
                               tattttatttTTATTCACGAGTGCATTACATCTTTTTAAAGGTTATGTGCCCGTTACGTTCTTTGTTGCTGAAACTTTGCTAATCGTTTTTACCATCATTTTAATTATTATTTTAACGACGGAACAAACATGGAAGcatca                                                                                                            <  1:454793/141‑1 (MQ=255)
                                 ttttAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATg                                                                                                          >  2:821842/1‑141 (MQ=255)
                                   ttAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGAc                                                                                                        <  1:392952/141‑1 (MQ=255)
                                    tAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAATCATGGAAGCATCATGAcc                                                                                                       <  2:61757/141‑1 (MQ=255)
                                        tataTTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGTAGCATCATGACCTATg                                                                                                   >  2:384579/1‑141 (MQ=255)
                                           atTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCg                                                                                                <  1:781508/141‑1 (MQ=255)
                                           atTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCg                                                                                                <  2:669664/141‑1 (MQ=255)
                                            ttAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGa                                                                                               <  4:325791/141‑1 (MQ=255)
                                               aCGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGt                                                                                            <  3:233406/141‑1 (MQ=255)
                                               aCGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGt                                                                                            <  3:415159/141‑1 (MQ=255)
                                                cGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTa                                                                                           >  3:380790/1‑141 (MQ=255)
                                                 gggttcATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCTACGTAt                                                                                          <  1:976765/136‑1 (MQ=255)
                                                 gAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTAt                                                                                          <  2:997935/141‑1 (MQ=255)
                                                     gCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTAtcgtc                                                                                      >  2:333879/1‑141 (MQ=255)
                                                     gCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTAtcgtc                                                                                      <  3:4914/141‑1 (MQ=255)
                                                          aaaTCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAgt                                                                                 >  3:81526/1‑141 (MQ=255)
                                                          aaaTCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAgt                                                                                 <  1:1009913/141‑1 (MQ=255)
                                                           aaTCTTTTTAAAGGTTATGTGGCCGATACGTTCTACATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAgtg                                                                                <  4:410939/141‑1 (MQ=255)
                                                            aTCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAgtgt                                                                               <  3:319432/141‑1 (MQ=255)
                                                               tttttAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAACCGCGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGtgttgt                                                                            <  3:447537/141‑1 (MQ=255)
                                                               tttttAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGtgttgt                                                                            <  2:445081/141‑1 (MQ=255)
                                                                ttttAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAGGCATCATGACCTATGGCGACGTATCGTCGAAGTgttgtt                                                                           <  4:307637/141‑1 (MQ=255)
                                                                ttttAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTgttgtt                                                                           <  2:1003057/141‑1 (MQ=255)
                                                                ttttAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGACGAAGTgttgtt                                                                           >  2:688442/1‑141 (MQ=255)
                                                                ttttAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATGTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTgttgtt                                                                           <  1:43901/141‑1 (MQ=255)
                                                                    aaaGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATTGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTAttg                                                                       <  2:793739/141‑1 (MQ=255)
                                                                     aaGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCGTCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTAttgt                                                                      >  2:790640/1‑141 (MQ=255)
                                                                     aaGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGAGCTATGGCGACGTATCGTCGAAGTGTTGTTAttgt                                                                      >  1:1003997/1‑141 (MQ=255)
                                                                      aGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTAttgtt                                                                     <  1:120008/141‑1 (MQ=255)
                                                                         ttATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTtgat                                                                  <  3:273048/141‑1 (MQ=255)
                                                                          tATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTtgatg                                                                 <  2:379217/141‑1 (MQ=255)
                                                                          tATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTtgatg                                                                 >  2:877945/1‑141 (MQ=255)
                                                                               ggCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGACAtt                                                            >  1:578604/1‑141 (MQ=255)
                                                                                  cGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGACATTAAc                                                         >  4:176470/1‑141 (MQ=255)
                                                                                       cGTTCTATATTGCTGAAACATTGCTAATCATTTTAACCATCATTTTAATTATCATTTTAACAACGGAACAAACATGGAAGcatcat                                                                                                           >  2:837889/1‑86 (MQ=255)
                                                                                       cGTTCTATATTGCTGAAACATTGCTAATCATTTTAACCATCATTTTAATTATCATTTTAACAACGGAACAAACATGGAAGcatcat                                                                                                           <  1:837889/86‑1 (MQ=255)
                                                                                       cGTTCTATATTGCTGAAACATTGCTAATCATTTTAACCATCATTTTAATTATCATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGGCATTAACAGGCa                                                    >  1:675156/1‑141 (MQ=255)
                                                                                        gTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGACATTAACAGGCaa                                                   >  2:281301/1‑141 (MQ=255)
                                                                                        gTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGACATTAACAGGCaa                                                   <  2:130178/141‑1 (MQ=255)
                                                                                        gTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGACAATAACAGGCaa                                                   >  1:275541/1‑141 (MQ=255)
                                                                                         ttCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGACATTAACAGGCAAc                                                  >  4:15295/1‑141 (MQ=255)
                                                                                          tCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGACATTAACAGGCAACg                                                 <  1:645258/141‑1 (MQ=255)
                                                                                            tataTTGCGGAAACATTGCTAATCATTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGACATTAACAGGCAACGTa                                               <  2:213181/141‑1 (MQ=255)
                                                                                             atatTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGACATTAACAGGCAACGTAt                                              >  1:1000686/1‑141 (MQ=255)
                                                                                             atatTGCTGAAACATTGCTAATCGTTTTAACCAGCATTTTAATTATTATTTTAGCAACGGAACTAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGACATTAACAGGCAACGTAt                                              <  4:299499/141‑1 (MQ=255)
                                                                                                 tGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGACATTAACAGGCAACGTATTTAc                                          <  4:246928/141‑1 (MQ=255)
                                                                                                    tGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTa                                                                                                                                      >  1:288140/1‑46 (MQ=255)
                                                                                                    tGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTa                                                                                                                                      <  2:288140/46‑1 (MQ=255)
                                                                                                        aCATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGACATTAACAGGCAACGTATTTACAttatta                                   >  3:164823/1‑141 (MQ=255)
                                                                                                        aCATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGACATTAACAGGCAACGTATTTACAttatta                                   >  3:164739/1‑141 (MQ=255)
                                                                                                           ttGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGACATTAACAGGCAACGTATTTACATTATTAATg                                >  1:779735/1‑141 (MQ=255)
                                                                                                            tGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGACATTAACAGGCAACGTATTTACATTATTAATGt                               >  1:189494/1‑141 (MQ=255)
                                                                                                             gCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGACATTAACAGGCCACGTCTTTACATTATTAATGtt                              >  2:575172/1‑141 (MQ=255)
                                                                                                             gCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGACATTAACAGGCAACGTATTTACATTATTAATGtt                              >  4:222483/1‑141 (MQ=255)
                                                                                                              cTAATCGTTTTACCCCTCATTTTAATTATTATTTTAACCACGGAACCAACCTGGAAGCCTCATGACCTATGGCGACGTATCGTCGCAGTGTTGTTTTTGTTGATGACATTAACAGGCAACGTATTTACATTATTAATGttt                             <  4:223768/141‑1 (MQ=255)
                                                                                                               tAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGACATTAACAGGCAACGTATTTACATTATTAATGTTTg                            <  2:1041893/141‑1 (MQ=255)
                                                                                                               tAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGACATTAACAGGCAACGTATTTACATTATTAATGTTTg                            >  1:359399/1‑141 (MQ=255)
                                                                                                                  tCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGACATTAACAGGCAACGTATTTACATTATTAATGTTTGTaa                         <  4:342267/141‑1 (MQ=255)
                                                                                                                    gTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGACATTAACAGGCAACGTATTTACATTATTAATGTTTGTAAGt                       <  2:880758/141‑1 (MQ=255)
                                                                                                                    gTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGACATTAACAGGCAACGTATTTACATTATTAATGTTTGTAAGt                       <  3:243810/141‑1 (MQ=255)
                                                                                                                    gTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGACATTAACAGGCAACGTATTTACATTATTAATGTTTGTAAGt                       >  1:438070/1‑141 (MQ=255)
                                                                                                                    gTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGACATTAACAGGCAACGTATTTACATTATTAATGTTTGTAAGt                       <  1:857670/141‑1 (MQ=255)
                                                                                                                      tttAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGACATTAACAGGCAACGTATTTACATTATTAATGTTTGTAAGTAt                     <  3:286756/141‑1 (MQ=255)
                                                                                                                      tgtAACCAGCGTTTTAAGTAGCATTGTAACAGCGGAACAAACAGGGAAGCATCATGACGTATGGCGACGTATCGTCGGAGTGTTGTTATTGTTGATGGCATTAACAGGCAACGTATTTACATTATTAATGTTTGTAAGTAt                     <  2:620255/139‑1 (MQ=255)
                                                                                                                       ttAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGACATTAACAGGCAACGTATTTACATTATTAATGTTTGTAAGTAtt                    >  3:47949/1‑141 (MQ=255)
                                                                                                                        tAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGACATTAACAGGCAACGTATTTACATTATTAATGTTTGTAAGTATTa                   >  2:741371/1‑141 (MQ=255)
                                                                                                                        tAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTCATTGTTGATGACATTAACAGGCAACGTATTTACATTATTAATGTTTGTACGTATTa                   >  4:367696/1‑141 (MQ=255)
                                                                                                                          aCCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGACATTAACAGGCAACGTATTTACATTATTAATGTTTGTAAGTATTAGa                 >  1:375738/1‑141 (MQ=255)
                                                                                                                          aCCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGACATTAACAGGCAACGTATTTACATTATTAATGTTTGTAAGTATTAGa                 >  1:159539/1‑141 (MQ=255)
                                                                                                                           ccATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGACATTAACAGGCAACGTATTTACATTATTAATGTTTGTAAGTATTAGAc                >  4:10978/1‑141 (MQ=255)
                                                                                                                           ccATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGACATTAACAGGCAACGTATTTACATTATTAATGTTTGTAAGTATTAGAc                <  2:786039/141‑1 (MQ=255)
                                                                                                                           ccATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGACATTAACAGGCAACGTATTTACATTATTAATGTTTGTAAGTATTAGAc                <  1:592703/141‑1 (MQ=255)
                                                                                                                             atcatTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGACATTAACAGGCAACGTATTTACATTATTAATGTTTGTAAGTATTAGACGt              <  2:488583/141‑1 (MQ=255)
                                                                                                                                  tttAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGACATTAACAGGCAACGTATTTACATTATTAATGTTTGTAAGTATTAGACGTTACCa         >  2:785403/1‑141 (MQ=255)
                                                                                                                                  tttAATTATCATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGGCATTAACAGGCAACGTATTTACATTATTAATGTTTGTAAGTATTAGACGTTACCa         >  3:39510/1‑141 (MQ=255)
                                                                                                                                     aaTTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGACATTAACAGGCAACGTATTTACATTATTAATGTTTGTAAGTATTAGACGTTACCAACg      >  3:278918/1‑141 (MQ=255)
                                                                                                                                      attattattTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGACATTAACAGGCAACGTATTTACATTATTAATGTTTGTAAGTATTAGACGTTACCAACGt     >  2:427851/1‑141 (MQ=255)
                                                                                                                                         attattTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGACATTAACAGGCAACGTATTTACATTATTAATGTTTGTAAGTATTAGACGTTACCAACGTACa  <  4:179256/141‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                          
AATTATTCGCAAGTTACGCCATAGCAACTTATATTTTAGTTATATTAACGAGTGCATTAAATCTTTTTAAAGGTTATGTGGCCGATACGTTCTATATTGCTGAAACATTGCTAATCGTTTTAACCATCATTTTAATTATTATTTTAACAACGGAACAAACATGGAAGCATCATGACCTATGGCGACGTATCGTCGAAGTGTTGTTATTGTTGATGACATTAACAGGCAACGTATTTACATTATTAATGTTTGTAAGTATTAGACGTTACCAACGTACA  >  USA300TCH1516_ALE/238034‑238311

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: