BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	267,872	T→C	6.2%	R525R (CGT→CGC)	USA300HOU_RS01175 →	Staphylocoagulase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	267,872	0	T	C	6.2%	309.5 / 4.0	123	R525R (CGT→CGC)	USA300HOU_RS01175	Staphylocoagulase
Reads supporting (aligned to +/- strand): ref base T (66/45); new base C (6/2); total (74/49)
Fisher's exact test for biased strand distribution p-value = 4.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01

Only 100 of 126 total aligned reads displayed.

AGAGATGCTGGTACAGGTATCCGTGAATACAACGATGGAACATTTGGATATGAAGCGAGACCAAGATTCAATAAGCCATCAGAAACAAATGCATATAACGTAACAACACATGCAAATGGTCAAGTATCATACGGAGCTCGTCCGACACAAAACAAGCCAAGCAAAACAAACGCATATAACGTAACAACACATGGAAACGGCCAAGTATCATATGGCGCTCGCCCAACACAAAACAAGCCAAGCAAAACAAATGCATACAACGTAACAACACATGCAAACGG  >  USA300TCH1516_ALE/267732‑268012

agagATGCTGGTACAGGTATCCGTGAATACAACGATGGAACATTTGGATATGAAGCGAGACCAAGATTCAATAAGCCATCAGAAACAAATGCATATAACGTAACAACACATGCAAATGGTCAAGTATCATACGGAGCTCGt                                                                                                                                              <  2:866506/141‑1 (MQ=255)

 gagaTGCTGGTACAGGTATCCGTGAATACAACGATGGAACATTTGGATATGAAGCGAGACCAAGATTCAATAAGCCATCAGAAACAAATGCATATAACGTAACAACACATGCAAATGGTCAAGTATCATACGGAGCTCGTc                                                                                                                                             >  2:516801/1‑141 (MQ=255)

  agaTGCTGGTACAGGTATCCGTGAATACAACGATGGAACATTTGGATATGACGCGAGACCAAGATTCAATAAGCCATCAGAAACAAATGCATATAACGTAACAACACATGCAAATGGTCAAGTATCATACGGAGCTCGTcc                                                                                                                                            >  2:1031967/1‑141 (MQ=255)

      gCTGGTACAGGTATCCGTGAATACAACGATGGAACATTTGGATATGAAGCGAGACCAAGATTCAATAAGCCATCAGAAACAAATGCATATAACGTAACAACACATGCAAATGGTCAAGTATCATACGGAGCTCGTCCGaca                                                                                                                                        >  1:878253/1‑141 (MQ=255)

       cTGGTACAGGTATCCGTGAATACAACGATGGAACATTTGGATATGAAGCGAGACCAAGATTCAATAAGCCATCAGAAACAAATGCATATAACGTAACAACACATGCAAATGGTCAAGTATCATACGGAGCTCGTCCGacac                                                                                                                                       <  1:716969/141‑1 (MQ=255)

       cTGGTACAGGTATCCGTGAATACAACGATGGAACATTTGGATATGAAGCGAGACCAAGACTCAATAAGCCATCAGAAACAAATGCATATAACGTAACAACACACGCAAATGGTCAAGTAACATACGGAGCTAGTCAGacac                                                                                                                                       >  2:406688/1‑141 (MQ=255)

        tGGTACAGGTATCCGTGAATACAACGATGGAACATTTGGATATGAAGCGAGACCAAGATTCAATAAGCCATCAGAAACAAATGCATATAACGTAACAACACATGCAAATGGTCAAGTATCATACGGAGCTCGTCCGAcaca                                                                                                                                      <  2:120159/141‑1 (MQ=255)

           tACAGGTATCCGTGAATACAACGATGGAACATTTGGATATGAAGCGAGACCAAGATTCAATAAGCCATCAGAAACAAATGCATATAAAGTAACAACACATGCAAATGGTCAAGTATCATACGGAGCTCGTCCGACACaaaa                                                                                                                                   >  3:409413/1‑141 (MQ=255)

            gcAGGTATCCGTGAATACAACGAGATAACATTTGGATAAAAAGCGAGACCAAGACGCAATAATCCTTCAGAAACAAATGCATATAACATAAAAACACATGCAAATGGGCAAATATCATATAGAACTCAACCTACaaaaaaa                                                                                                                                  >  4:33080/2‑140 (MQ=255)

            aCAGGTATCCGTGAATACAACGATGGAACATTTGGATATGAAGCGAGACCAAGATTCAATAAGCCATCAGAAACAAATGCATATAACGTAACAACACATGCAAATGGTCAAGTATCATACGGAGCTCGTCCGACACAAAAc                                                                                                                                  >  4:83518/1‑141 (MQ=255)

              aGGTATCCGTGAATACAACGATGGAACATTTGGATATGAAGCGAGACCAAGATTCAATAAGCCATCAGAAACAAATGCATATAACGTAACAACACATGCAAATGGTCAAGTATCATACGGAGCTCGTCCGACACAAAAcaa                                                                                                                                >  1:893931/1‑141 (MQ=255)

               ggTATCCGTGAATACAACGATGGAACATTTGGATATGAAGCGAGACCAAGATTCAATAAGCCATCAGAAACAAATGCATATAACGTAACAACACATGCAAATGGTCAAGTATCATACGGAGCTCGTCCGACACAAAAcaag                                                                                                                               <  1:51590/141‑1 (MQ=255)

               ggTATCCGTGAATACAACGATGGAACATTTGGATATGAAGCGAGACCAAGATTCAATAAGCCATCAGAAACAAATGCATATAACGTAACAACACATGCAAATGGTCAAGTATCATACGGAGCTCGTCCGACACAAAAcaag                                                                                                                               >  3:148991/1‑141 (MQ=255)

                   tCCGTGAATACAACGATGGAACATTTGGATATGAAGCGAGACCAAGATTCAATAAGCCATCAGAAACAAATGCATATAACGTAACAACACATGCAAATGGTCAAGTATCATACGGAGCTCGTCCGACACAAAAcaagccaa                                                                                                                           >  4:212433/1‑141 (MQ=255)

                      gTGAATACAACGATGGAACATTTGGATATGAAGCGAGACCAAGATTCAATAAGCCATCAGAAACAAATGCATATAACGTAACAACACATGCAAATGGTCAAGTATCATACGGAGCTCGTCCGACACAAAACAAGCCAAGCa                                                                                                                        >  2:779250/1‑141 (MQ=255)

                       tGAATACAACGATGGAACATTTGGATATGAAGCGAGACCAAGATTCAATAAGCCATCAGAAACAAATGCATATAACGTAACAACACATGCAAATGGTCAAGTATCATACGGAGCTCGTCCGACACAAAACAAGCCAAGCaa                                                                                                                       <  4:184238/141‑1 (MQ=255)

                         aaTACAACGATGGAACATTTGGATATGAAGCGAGACCAAGATTCAATAAGCCATCAGAAACAAATGCATATAACGTAACAACACATGCAAATGGTCAAGTATCATACGGAGCTCGTCCGACACAAAACAAGCCAAGCaaaa                                                                                                                     >  1:4791/1‑141 (MQ=255)

                          aTACAACGATGGAACATTTGGATATGAAGCGAGACCAAGATTCAATAAGCCATCAGAAACAAATGCATATAACGTAACAACACATGCAAATGGTCAAGTATCATACGGAGCTCGTCCGACACAAAACAAGCCAAGCAaaac                                                                                                                    >  2:987849/1‑141 (MQ=255)

                             cAACGATGGAACATTTGGATATGAAGCGAGACCAAGATTCAATAAGCCATCAGAAACAAATGCATATAACGTAACAACACATGCAAATGGTCAAGTATCATACGGAGCTCGTCCGACACAAAACAAGCCAAGCAaaacaaa                                                                                                                 >  1:1082420/1‑141 (MQ=255)

                               aCGATGGAACATTTGGATATGAAGCGAGACCAAGATTCAATAAGCCATCAGAAACAAATGCATATAACGTAACAACACATGCAAATGGTCAAGTATCATACGGAGCTCGTCCGACACAAAACAAGCCAAGCAAAACAAACg                                                                                                               >  2:171284/1‑141 (MQ=255)

                                ggagtgAACATTTGGATATAAAGTGAGACCAAGATTAAATAAGACAACAGAAAAAAAGGCATATAACATAACAACACATGCAAATGGTAAAGTATCATACGATGCGCGTCCGGCACAAAAAAAAACAAAAAAAACAAACGc                                                                                                              <  2:201076/136‑1 (MQ=255)

                                          tttGGATATGAAGCGAGACCAAGATTCAATAAGCCATCAGAAACAAATGCATCTAACGTAACAACACATGCAAATGGTCAAGTATCATACGGAGCTCGTCCGACACAAAACAAGCCAAGCAAAACAAACGCATATAACGTa                                                                                                    >  1:1061860/1‑141 (MQ=255)

                                           ttGGATATGAAGCGAGACCAAGATTCAATAAGCCATCAGAAACAAATGCATATAACGTAACAACACATGCAAATGGTCAAGTATCATACGGAGCTCGTCCGACACAAAACAAGCCAAGCAAAACAAACGCATATAACGTaa                                                                                                   >  2:489543/1‑141 (MQ=255)

                                            tGGATATGAAGCGAGACCAAGATTCAATAAGCCATCAGAAACAAATGCATATAACGTAACAACACATGCAAATGGTCAAGTATCATACGGAGCTCGTCCGACACAAAACAAGCCAAGCAAAACAAACGCATATAACGTaac                                                                                                  <  2:878253/141‑1 (MQ=255)

                                                  tGAAGCGAGACCAAGATTCAATAAGCCATCAGAAACAAATGCATATAACGTAACAACACATGCAAATGGTCAAGTATCATACGGAGCTCGTCCGACACAAAACAAGCCAAGCAAAACAAACGCATATAACGTAACAAcaca                                                                                            >  1:646175/1‑141 (MQ=255)

                                                  tGAAGCGAGACCAAGATTCAATAAGCCATCAGAAACAAATGCATATAACGTAACAACACATGCAAATGGTCAAGTATCATACGGAGCTCGTCCGACACAAAACAAGCCAAGCAAAACAAACGCATATAACGTAACAAcaca                                                                                            >  1:306028/1‑141 (MQ=255)

                                                  tGAAGCGAGACCAAGATTCAATAAGCCATCAGAAACAAATGCATATAACGTAACAACACATGCAAATGGTCAAGTATCATACGGAGCTCGTCCGACACAAAACAAGCCAAGCAAAACAAACGCATACAACGTAACAAcaca                                                                                            >  2:888688/1‑141 (MQ=255)

                                                        gagaCCAAGATTCAATAAGCCATCAGAAACAAATGCATATAACGTAACAACACATGCAAATGGTCAAGTATCATACGGAGCTCGTCCGACACAAAACAAGCCAAGCAAAACAAACGCATATAACGTAACAACACATGGaaa                                                                                      <  1:712968/141‑1 (MQ=255)

                                                        gagaCCAAGATTCAATAAGCCATCAGAAACAAATGCATATAACGTAACAACACATGCAAATGGTCAAGTATCATACGGAGCTCGTCCGACACAAAACAAGCCAAGCAAAACAAACGCATATAACGTAACAACACATGGaaa                                                                                      >  3:5277/1‑141 (MQ=255)

                                                         agaCCAAGATTCAATAAGCCATCAGAAACAAATGCATATAACGTAACAACACATGCAAATGGTCAAGTATCATACGGAGCTCGTCCGACACAAAACAAGCCAAGCAAAACAAACGCATATAACGTAACAACACATGGAAAc                                                                                     >  3:144486/1‑141 (MQ=255)

                                                          aaCCAAGATTCAATAAGCCATCAGAAACAAATGCATATAACGTAACAACACATGCAAATGGTCAAGTATCATACGGAGCTCGTCCGACACAAAACAAGCCAAGCAAAACAAACGCATATAACGTAACAACACATGGAAACg                                                                                    <  2:735063/140‑1 (MQ=255)

                                                           aCCAAGATTCAATAAGCCATCAGAAACAAATGCATATAACGTAACAACACATGCAAATGGTCAAGTATCATACGGAGCTCGTCCGACACAAAACAAGCCAAGCAAAACAAACGCATATAACGTAACAACACATGGAAACgg                                                                                   <  1:777950/141‑1 (MQ=255)

                                                           aCCAAGATTCAATAAGCCATCAGAAACAAATGCATATAACGTAACAACACATGCAAATGGTCAAGTATCATACGGAGCTCGTCCGACACAAAACAAGCCAAGCAAAACAAACGCATATAACGTAACAACACATGGAAACgg                                                                                   <  2:721617/141‑1 (MQ=255)

                                                            ccAAGATTCAATAAGCCATCAGAAACAAATGCATATAACGTAACAACACATGCAAATGGTCAAGTATCATACGGAGCTCGTCCGACACAAAACAAGCCAAGCAAAACAAACGCATATAACGTAACAACACATGGAAACGGc                                                                                  >  1:629403/1‑141 (MQ=255)

                                                               aGATTCAATAAGCCATCAGAAACAAATGCATATAACGTAACAACACATGCAAATGGTCAAGTATCATACGGAGCTCGTCCGACACAAAACAAGCCAAGCAAAACAAACGCATATAACGTAACAACACATGGAAACGGCCaa                                                                               >  1:184060/1‑141 (MQ=255)

                                                                gATTCAATAAGCCATCAGAAACAAATGCATATAACGTAACAACACATGCAAATGGTCAAGTATCATACGGAGCTCGTCCGACACAAAACAAGCCAAGCAAAACAAACGCATATAACGTAACAACACATGGAAACGGCCAAg                                                                              <  3:329342/141‑1 (MQ=255)

                                                                 aTTCAATAAGCCATCAGAAACAAATGCATATAACGTAACAACACATGCAAATGGTCAAGTATCATACGGAGCTCGTCCGACACAAAACAAGCCAAGCAAAACAAACGCATATAACGTAACAACACATGGAAACGGCCAAGt                                                                             >  1:359352/1‑141 (MQ=255)

                                                                  ttCAATAAGCCATCAGAAACAAATGCATATAACGTAACAACACATGCAAATGGTCAAGTATCATACGGAGCTCGTCCGACATACAAGAAGCCAAGCGAAACGAATGCATACAATGTAACAACACATGCAAACGGCCAAGTa                                                                            >  1:622156/1‑141 (MQ=14)

                                                                  ttCAATAAGCCATCAGAAACAAATGCATATAACGTAACAACACATGCAAATGGTCAAGTATCATACGGAGCTCGTCCGACACAAAACAAGCCAAGCAAAACAAACGCATATAACGTAACAACACATGGAAACGGCCAAGTa                                                                            <  1:865302/141‑1 (MQ=255)

                                                                   tCAATAAGCCATCAGAAACAAATGCATATAACGTAACAACACATGCAAATGGTCAAGTATCATACGGAGCTCGTCCGACACAAAACAAGCCAAGCAAAACAAACGCATATAACGTAACAACACATGGAAACGGCCAAGTAt                                                                           <  3:274601/141‑1 (MQ=255)

                                                                     aaTAAGCCATCAGAAACAAATGCATATAACGTAACAACACATGCAAATGGTCAAGTATCATACGGAGCTCGTCCGACACAAAACAAGCCAAGCAAAACAAACGCATATAACGTAACAACACATGGAAACGGCCAAGTATCa                                                                         <  1:182337/141‑1 (MQ=255)

                                                                      aTAAGCCATCAGAAACAAATGCATATAACGTAACAACACATGCAAATGGTCAAGTATCATACGGAGCTCGTCCGACACAAAACAAGCCAAGCAAAACAAACGCATATAACGTAACAACACATGGAAACGGCCAAGTATCat                                                                        >  2:547052/1‑141 (MQ=255)

                                                                      aTAAGCCATCAGAAACAAATGCATATAACGTAACAACACATGCAAATGGTCAAGTATCATACGGAGCTCGTCCGACACAAAACAAGCCAAGCAAAACAAACGCATATAACGTAACAACACATGGAAACGGCCAAGTATCat                                                                        <  1:46653/141‑1 (MQ=255)

                                                                      aTAAGCCATCAGAAACAAATGCATATAACGTAACAACACATGCAAATGGTCAAGTATCATACGGAGCTCGTCCGACACAAAACAAGCCAAGCAAAACAAACGCATACAACGTAACAACACATGCAAACGGCCAAGTATCaa                                                                        >  4:84400/1‑140 (MQ=255)

                                                                      aTAAGCCATCAGAAACAAATGCATATAACGTAACAACACATGCAAATGGTCAAGTATCATACGGAGCTCGCCCGACACACAACAAGCCAAGCAAAACAAACGCATACAACGTAACAACACATGCAGACGGCCAAGCAACat                                                                        >  2:1054070/1‑141 (MQ=18)

                                                                      aTAAGCCATCAGAAACAAATGCATATAACGTAACAACACATGCAAATGGTAAAGTATCATACGGAGCACGTCCGACACAAAACAAGCCAAGCAAAACAAACACATAAAACGAAACAACACATGGAAAAGGAAAAGTATCaa                                                                        >  2:438658/1‑140 (MQ=21)

                                                                       tAAGCCATCAGAAACAAATGCATATAACGTAACAACACATGCAAATGTTCAAGTATCATACGGAGCTCGTCCGACACAAAACAAGCCAAGCAAAACAAACGCATATAACGTAACAACACATGGAAACGGCCAAGTATCata                                                                       <  4:274857/141‑1 (MQ=255)

                                                                       tAAGCCATCAGAAACAAATGCATATAACGTAACAACACATGCAAATGGTCAAGTATCATACGGAGCTCGTCCGACACAAAACAAGCCAAGCAAAACAAACGCATATAACGTAACAACACATGGAAACGGCCAAGTATCata                                                                       >  1:129846/1‑141 (MQ=255)

                                                                          gCCATCAGAAACAAATGCATATAACGTAACAACACATGCAAATGGTCAAGTATCATACGGAGCTCGTCCGACACAAAACAAGCCAAGCAAAACAAACGCATATAACGTAACAACACATGGAAACGGCCAAGTATCATATgg                                                                    <  1:634847/141‑1 (MQ=255)

                                                                           ccATCAGAAACAAATGCATATAACGTAACAACACATGCAAATGGTCAAGTATCATACGGAGCTCGTCCGACACAAAACAAGCCAAGCAAAACAAACGCATATAACGTAACAACACATGCAAACGGCCAAGTATCATATggg                                                                   >  4:307318/1‑140 (MQ=255)

                                                                             aTCAGAAACAAATGCATATAACGTAACTACTTATTCATGTTGCGAAGAACCATGCGGTCCTCGCCCGACGCAAAACAAGCCCAGTACACGAAACTCATACAACGTAACTACCCCCAAAGACGGCCAATTATCATATGgctc                                                                 <  4:342298/141‑3 (MQ=255)

                                                                              tCAGAAACAAATGCATATAACGTAACAACACATGCAAATGGTCAAGTATCATACGGAGCTCGTCCGACACAAAACAAGCCAAGCAAAACAAACGCATATAACGTAACAACACATGGAAACGGCCAAGTAACATATGGCGCt                                                                >  4:275268/1‑141 (MQ=255)

                                                                               cAGAAACAAATGCATATAACGTAACAACACATGCAAATGGTCAAGTATCATACGGAGCTCGCCCGACACAAAACAAGCCAAGCAAAACAAACGCATACAACGTAACAACACATGCAAACGGCCAAGTATCATATGGCCCTc                                                               >  2:530897/1‑141 (MQ=255)

                                                                                aGAAACAAATGCATAGAACGTAACAACACATGCAAATGGTCAAGTATCATACGGAGCTCGTCCGACACAAAACAAGCCAAGCAAAACAAACGCATATAACGTAACAACACATGGAAACGGCCAAGTATCATATGGCGCTCg                                                              <  1:557424/141‑1 (MQ=255)

                                                                                     cAAATGCATACAACGTAACAACACATGCAAATGGTCAAGTATCATATGGCGCTCGCCCGACACAAAAAAAGCCAAGCAAAACAAACGCATATAACGTAACAACACATGCAGATGGTAACGTAACATACGGAGCTCGcgaaa                                                         >  4:223452/1‑137 (MQ=11)

                                                                                       aaggCATATAACGTAACAACACATGCAAATGGTCAAGTATCATACGGAGCTCGTCCGACACAAAACAAGCCAAGCAAAACAAACGCATATAACGTAACAACACATGGAAACGGCCAAGTATCATATGGCGCTCGCCCAaca                                                       <  2:258093/138‑1 (MQ=255)

                                                                                       aaTGCATATAACGTAACAACACATGCAAATGGTCAAGTATCATACGGAGCTCGTCCGACACAAAACAAGCCAAGCAAAACAAACGCATATAACGTAACAACACATGGAAACGGCCAAGTATCATATGGCGCTCGCCCAaca                                                       <  1:939175/141‑1 (MQ=255)

                                                                                       aaTGCATATAACGTAACAACACATGCAAATGGTCAAGTATCATACGGAGCTCGTCCGACACAAAACAAGCCAAGCAAAACAAACGCATATAACGTAACAACACATGGAAACGGCCAAGTATCATATGGCGCTCGCCCAaca                                                       >  3:404538/1‑141 (MQ=255)

                                                                                        aTGCATATAACGTAACAACACATGCAAATGGTCAAGTATCATACGGAGCTCGTCCGACACAAAACAAGCCAAGCAAAACAAACGCATATAACGTAACAACACATGGAAACGGCCAAGTATCATATGGCGCTCGCCCAacac                                                      >  3:130313/1‑141 (MQ=255)

                                                                                          gCATATAACGTAACAACACATGCAAATGGTCAAGTATCATACGGAGCTCGTCCGACACAAAACAAGCCAAGCAAAACAAACGCATATAACGTAACAACACATGGAAACGGCCAAGTATCATATGGCGCTCGCCCAACACaa                                                    >  3:97208/1‑141 (MQ=255)

                                                                                           cATATAACGTAACAACACATGCAAATGGTCAAGTATCATACGGAGCTCGTCCGACACAAAACAAGCCAAGCAAAACAAACGCATATAACGTAACAACACATGGAAACGGCCAAGTAACATATGGCGCTCGCCCAACACaaa                                                   >  2:427951/1‑141 (MQ=255)

                                                                                               taACGTAACAACACATGCAAATGGTCAAGTATCATACGGAGCTCGTCCGACACAAAACAAGCCAAGCAAAACAAACGCATATAACGTAACAACACATGGAAACGGCCAAGTATCATATGGCGCTCGCCCAACACAAAAcaa                                               >  1:410174/1‑141 (MQ=255)

                                                                                                 aCGTAACAACACATGCAAATGGTCAAGTATCATACGGTGCTCGCCCAACACAAAAAAAGCCAAGCAAAACAAATGCATACAACGTAACAACACATGCAAATGGTCAAGTATCATATGGCGCTCGCCCGACACAAAAaaagc                                             <  3:277876/141‑1 (MQ=11)

                                                                                                 aCGTAACAACACATGCAAATGATTAAGTATCATACGGTGCTAGCATAAAAAAAAAAAAGTAAAGCGAAATAAATGAATAAAACGTAAAAATACATGAAAATGGTAAGGTGTCATATGGCGATAGAAAAACAAAAAAaaagc                                             >  4:106448/1‑141 (MQ=255)

                                                                                                     aacaacACATGCAAATGGTCAAGTATCATACGGAGCTCGTCCGACACAAAACAAGCCAAGCAAAACAAACGCATATAACGTAACAACACATGGAAACGGCCAAGTATCATATGGCGCTCGCCCAACACAAAAcaagccaag                                         <  3:40253/141‑1 (MQ=255)

                                                                                                     aacaacACATGCAAATGGTCAAGTATCATACGGAGCTCGTCCGACACAAAACAAGCCAAGCAAAACAAACGCATATAACGTAACAACACATGGAAACGGCCAAGTATCATATGGCGCTCGCCCAACACAAAAcaagccaag                                         >  2:372838/1‑141 (MQ=255)

                                                                                                     aacaacACAAGCAAATGGTCAAGTATAATACGAAGCTCGACCGACACAAAACAAGCCAAGCAAAACAAACGCATATAACGTAACAACACATGGAAACGACCAATTATCATATGTCACCCGCCCAACAAAAAAccagtcaaa                                         >  2:634518/1‑140 (MQ=21)

                                                                                                      acgacacATGCAAATGGTCAAGTATCATACGGAGCTCGTCCGACACAAAACAAGCCAAGCAAAACAAACGCATATAACGTAACAACACATGGAAACGACCAAGTATCATATGGCGCTCGCCCAACACAAAAcaagccaagc                                        <  4:177808/138‑1 (MQ=255)

                                                                                                      acaacaCATGCAAATGGTCAAGTATCATACGGAGCTCGTCCGACACAAAACAAGCCAAGCAAAACAAACGCATATAACGTAACAACACATGGAAACGGCCAAGTATCATATGGCGCTCGCCCAacac                                                      >  2:536764/1‑127 (MQ=255)

                                                                                                      acaacaCATGCAAATGGTCAAGTATCATACGGAGCTCGTCCGACACAAAACAAGCCAAGCAAAACAAACGCATATAACGTAACAACACATGGAAACGGCCAAGTATCATATGGCGCTCGCCCAacac                                                      <  1:536764/127‑1 (MQ=255)

                                                                                                      acaacaCATGCAAATGGTCAAGTATCATACGGAGCTCGTCCGACACAAAACAAGCCAAGCAAAACAAACGCATATAACGTAACAACACATGGAAACGGCCAAGTATCATATGGCGCTCGCCCAACACAAAAcaagccaagc                                        <  1:420536/141‑1 (MQ=255)

                                                                                                       caacaCATGCAAATGGTCAAGTATCATACGGAGCTCGTCCGACACAAAACAAGCCAAGCAAAACAAACGCATATAACGTAACAACACATGGAAACGGCCAAGTATCATATGGCGCTCGCCCAACACAAAACAAGCCAAGCa                                       <  2:137232/141‑1 (MQ=255)

                                                                                                        aacaCATGCAAATGGTCAAGTATCATACGGAGCTCGTCCGACACAAAACAAGCCAAGCAAAACAAACGCATATAACGTAACAACACATGGAAACGGCCAAGTATCATATGGCGCTCGCCCAACACAAAACAAGCCAAGCaa                                      <  3:117106/141‑1 (MQ=255)

                                                                                                        aacaCATGCAAATGGTCAAGTATCATACGGAGCTCGTCCGACACAAAACAAGCCAAGCAAAACAAACGCATATAACGTAACAACACATGGAAACGGCCAAGTATCATATGGCGCTCGCCCAACACAAAACAAGCCAAGCaa                                      <  3:76772/141‑1 (MQ=255)

                                                                                                            caTGCAAATGGTCAAGTATCATACGGAGCTCGTCCGACACAAAACAAGCCAAGCAAAACAAACGCATATAACGTAACAACACATGGAAACGGCCAAGTATCATATGGCGCTCGCCCAACACAAAACAAGCCAAGCAAAACa                                  >  1:533247/1‑141 (MQ=255)

                                                                                                              tGCAAATGGTCAAGTATCATACGGAGCTCGTCCGACACAAAACAAGCCAAGCAAAACAAACGCATATAACGTAACAACACATGGAAACGGCCAAGTATCATATGGCGCTCGCCCAACACAAAACAAGCCAAGCAAAACaaa                                <  1:804236/141‑1 (MQ=255)

                                                                                                               gCAAATGGTCAAGTATCATACGGAGCTCGTCCGACACAAAACAAGCCAAGCAAAACAAACGCATATAACGTAACAACACATGGAAACGGCCAAGTATCATATGGCGCTCGCCCAACACAAAACAAGCCAAGCAAAACAAAt                               <  2:825451/141‑1 (MQ=255)

                                                                                                                 aaaTGGTCAAGTATCATACGGAGCTCGTCCGACACAAAACAAGCCAAGCAAAACAAACGCATATAACGTAACAACACATGGAAACGGCCAAGTATCATATGGCGCTCGCCCAACACAAAACAAGCCAAGCAAAACAAATGc                             >  4:274627/1‑141 (MQ=255)

                                                                                                                  aaTGGTCAAGTATCATACGGAGCCCGACCGACACAAAACAAGCCAAGCAAAACAAACGCATATAAAGGAACAACACATGGAAACGGCCAAGTATCATATGGCGCTCGCCCAACACAAAACAAGCCAATCAAAACAAATGCa                            <  2:112166/141‑1 (MQ=25)

                                                                                                                    tGGTCAAGTATCATACGGAGCTCGTCCGACACAAAACAAGCCAAGCAAAACAAACGCATATAACGTAACAACACATGGAAACGGCCAAGTATCATATGGCGCTCGCCCAACACAAAACAAGCCAAGCAAAACAAATGCATa                          <  3:127792/141‑1 (MQ=255)

                                                                                                                    tGGTCAAGTATCATACGGAGCTCGTCCGACACAAAACAAGCCAAGCAAAACAAACGCATATAACGTAACAACACATGGAAACGGCCAAGTATCATATGGCGCTCGCCCAACACAAAACAAGCCAAGCAAAACAAATGCATa                          >  4:327702/1‑141 (MQ=255)

                                                                                                                      gTCAAGTATCATACGGAGCTCGTCCGACACAAAACAAGCCAAGCAAAACAAACGCATATAACGTAACAACACATGGAAACGGCCAAGTATCATATGGCGCTCGCCCAACACaaa                                                   <  1:366538/114‑1 (MQ=255)

                                                                                                                      gTCAAGTATCATACGGAGCTCGTCCGACACAAAACAAGCCAAGCAAAACAAACGCATATAACGTAACAACACATGGAAACGGCCAAGTATCATATGGCGCTCGCCCAACACaaa                                                   >  2:366538/1‑114 (MQ=255)

                                                                                                                         aaGTATCATACGGAGCTCGTCCGACACAAAACAAGCCAAGCAAAACAAACGCATATAACGTAACAACACATGGAAACGGCCAAGTATCATATGGCGCTCGCCCAACACAAAACAAGCCAAGCAAAACAAATGCATACAACg                     <  1:597167/141‑1 (MQ=255)

                                                                                                                           gTATCATACGGAGCTCGTCCGACACAAAACAAGCCAAGCAAAACAAACGCATATAACGTAACAACACATGGAAACGGCCAAGTATCATATGGCGCTCGCCCAACACAAAACAAGCCAAGCAAAACAAATGCATACAACGTa                   <  2:1003896/141‑1 (MQ=255)

                                                                                                                                aTACGGAGCTCGTCCGACACAAAACAAGCCAAGCAAAACAAACGCATATAACGTAACAACACATGGAAACGGCCAAGTATCATATGGCGCTCGCCCAACACAAAACAAGCCAAGCAAAACAAATGCATACAACGTaacaac              >  1:206667/1‑141 (MQ=255)

                                                                                                                                aTACGGAGCACGACCGACACAAAACAAGCCAAGCAAAACAAACGCATATAACGTAACAACACATGGAAACGGCCAAGTATCATATGGCGCTCGCCCAACACAAAACAAGCCAAGCAAAACAAATGCATACAACGTaacaac              <  2:225660/141‑1 (MQ=255)

                                                                                                                                  aCGGAGCTCGTCCGACACAAAACAAGCCAAGCAAAACAAACGCATATAACGTAACAACACATGGAAACGGCCAAGTATCATATGGCGCTCGCCCAACACAAAACAAGCCAAGCAAAACAAATGCATACAACGTAACAacac            <  4:48746/141‑1 (MQ=255)

                                                                                                                                  aCGGAGCTCGTCCGACACAAAACAAGCCAAGCAAAACAAACGCATATAACGTAACAACACATGGAAACGGCCAAGTATCATATGGCGCTCGCCCAACACAAAACAAGCCAAGCAAAACAAATGCATACAACGTAACAacac            <  1:1039778/141‑1 (MQ=255)

                                                                                                                                  aCGGAGCTCGTCCGACACAAAACAAGCCAAGCAAAACAAACGCATATAACGTAACAACACATGGAAACGGCCAAGTATCATATGGCGCTCGCCCAACACAAAACAAGCCAAGCAAAACAAATGCATACAACGTAACAacac            >  1:223549/1‑141 (MQ=255)

                                                                                                                                    ggAGCTCGTCCGACACAAAACAAGCCAAGCAAAACAAACGCATATAACGTAACAACACATGGAAACGGCCAAGTATCATATGGCGCTCGCCCAACACAAAACAAGCCAAGCAAAACAAATGCATACAACGTAACAACACAt          >  2:438153/1‑141 (MQ=255)

                                                                                                                                     gAGCTCGTCCGACACAAAACAAGCCAAGCAAAACAAACGCATATAACGTAACAACACATGGAAACGGCCAAGTATCATATGGCGCTCGCCCAACACAAAACAAGCCAAGCAAAACAAATGCATACAACGTAACAACACATg         >  2:996279/1‑141 (MQ=255)

                                                                                                                                     gAGCCCTTCGGACACAAAACAAGCCAAACAAAACAAACGCATAAAGCGTAACAACACAAGGAAACGGCCAAGTATCATATGGCGCTCGCCCAACACAAAACAAGCCAAGCAAAACAAATGCATACAACGTAACAACACATg         <  2:999769/141‑1 (MQ=21)

                                                                                                                                       gCTCGTCCGACACAAAACAAGCCAAGCAAAACAAACGCATATAACGTAACAACACATGGAAACGGCCAAGTATCATATGGCGCTCGCCCAACACAAAACAAGCCAAGCAAAACAAATGCATACAACGTAACAACACATGCa       >  4:271555/1‑141 (MQ=255)

                                                                                                                                       gCTCGTCCGACACAAAACAAGCCAAGCAAAACAAACGCATATAACGTAACAACACATGGAAACGGCCAAGTATCATATGGCGCTCGCCCAACACAAAACAAGCCAAGCAAAACAAATGCATACAACGTAACAACACATGCa       >  1:449896/1‑141 (MQ=255)

                                                                                                                                       gCTCGTCCGACACAAAACAAGCCAAGCAAAACAAACGCATATAACGTAACAACACATGGAAACGGCCAAGTATCATATGGCGCTCGCCCAACACAAAACAAGCCAAGCAAAACAAATGCATACAACGTAACAACACATGCa       >  2:234416/1‑141 (MQ=255)

                                                                                                                                         tCGTCCGACACAAAACAAGCCAAGCAAAACAAACGCATATAACGTAACAACACATGGAAACGGCCAAGTATCATATGGCGCTCGCCCAACACAAAACAAGCCAAGCAAAACAAATGCATACAACGTAACAACACATGCaaa     <  1:970633/141‑1 (MQ=255)

                                                                                                                                          cGTCCGACACAAAACAAGCCAAGCAAAACAAACGCATATAACGTAACAACACATGGAAACGGCCAAGTATCATATGGCGCTCGCCCAACACAAAACAAGCCAAGCAAAACAAATGCATACAACGTAACAACACATGCAAAc    <  1:287918/141‑1 (MQ=255)

                                                                                                                                            tCCGACACAAAACAAGCCAAGCAAAACAAACGCATATAACGTAACAACACATGGAAACGGCCAAGTATCATATGGCGCTCGCCCAACACAAAACAAGCCAAGCAAAACAAATGCATACAACGTAACAACACATGCAAACgg  >  3:69574/1‑141 (MQ=255)

                                                                                                                                            tCCGACACAAAACAAGCCAAGCAAAACAAACGCATATAACGTAACAACACATGGAAACGGCCAAGTATCATATGGCGCTCGCCCAACACAAAACAAGCCAAGCAAAACAAATGCATACAACGTAACAACACATGCAAACgg  >  1:634708/1‑141 (MQ=255)

AGAGATGCTGGTACAGGTATCCGTGAATACAACGATGGAACATTTGGATATGAAGCGAGACCAAGATTCAATAAGCCATCAGAAACAAATGCATATAACGTAACAACACATGCAAATGGTCAAGTATCATACGGAGCTCGTCCGACACAAAACAAGCCAAGCAAAACAAACGCATATAACGTAACAACACATGGAAACGGCCAAGTATCATATGGCGCTCGCCCAACACAAAACAAGCCAAGCAAAACAAATGCATACAACGTAACAACACATGCAAACGG  >  USA300TCH1516_ALE/267732‑268012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`