| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 355,692 | G→T | 6.2% | V336V (GTC→GTA) | brnQ_2 ← | Branched‑chain amino acid transport system 2 carrier protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 355,692 | 0 | G | T | 6.2% | 503.5 / 10.7 | 151 | V336V (GTC→GTA) | brnQ_2 | Branched‑chain amino acid transport system 2 carrier protein |
| Reads supporting (aligned to +/- strand): ref base G (70/71); new base T (7/3); total (77/74) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.28e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 154 total aligned reads displayed.
AGTATTTGTAAAATTGAAATAATCAATGTAATAACAGTTGCGAGTCGATAGGCCCAACTGAATCTGAATGTGCTAAACATG‑TTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGACGAGTCCTAT > USA300TCH1516_ALE/355552‑355832 | aGTATTTGTAAAATTGAAATAATCAATGTAATAACAGTTGCGAGTCGATAGGCCCAACTGAATCTGAATGTGCTAAACATG‑TTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATggg > 1:990235/1‑141 (MQ=255) gTATTTGTAAAATTGAAATAATCAATGTAATAACAGTTGCGAGTCGATAGGCCCAACTGAATCTGAATGTGCTAAACATG‑TTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGa < 2:569332/141‑1 (MQ=255) gTATTTGTAAAATTGAAATAATCAATGTAATAACAGTTGCGAGTCGATAGGCCCAACTGAATCTGAATGTGCTAAACATG‑TTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGa < 2:872493/141‑1 (MQ=255) tttGTAAAATTGAAATAATCAATGTAATAACAGTTGCGAGTCGATAGGCCCAACTGAATCTGAATGTGCTAAACATG‑TTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAg > 1:231211/1‑141 (MQ=255) tttGTAAAATTGAAATAATCAATGTAATAACAGTTGCGAGTCGATAGGCACAACTGAATCTGAACGTGCTAAACATG‑TTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAg < 4:437894/141‑1 (MQ=255) ttGTAAAATTGAAATAATCAATGTAATAACAGTTGCGAGTCGATAGGCCCAACTGAATCTGAATGTGCTAAACATG‑TTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGc > 2:332395/1‑141 (MQ=255) tGTAAAATTGAAATAATCAATGTAATAACAGTTGCGAGTCGATAGGCCCAACTGAATCTGAATGTGCTAAACATG‑TTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCa < 1:157074/141‑1 (MQ=255) aaTTGAAATAATCAATGTAATAACAGTTGCGAGTCGATAGGCCCAACTGAATCTGAATGTGCTAAACATG‑TTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAAtttt > 3:289802/1‑141 (MQ=255) ttGAAATAATCAATGTAATAACAGTTGCGAGTCGATAGGCCCAACTGAATCTGAATGTGCTCCCCATG‑TTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTa < 3:317256/141‑1 (MQ=255) tGAAATAATCAATGTCATAACAGTTGCGAGTCGATAGGCCCAACTGAATCTGAATGTGCTAAACATG‑TTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTaa < 1:901425/141‑1 (MQ=255) aaaTAATCAATGTAATAACAGTTGCGAGTCGATAGGCCCAACTCAATCTGAATGTGCTAAACATG‑TTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTaaaa < 4:425513/141‑1 (MQ=255) aataatCAATGTAATAACAGTTGCGAGTCGATAGGCCCAACTGAATCTGAATGTGCTAAACATG‑TTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAAt > 2:878973/1‑141 (MQ=255) aataatCAATGTAATAACAGTTGCGAGTCGATAGGCCCAACTGAATCTGAATGTGCTAAACATG‑TTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAAt < 4:91591/141‑1 (MQ=255) aataatCAATGTAATAACAGTTGCGAATCGATAGGCCCAACTGAATCTGAATGTGCTAAACATG‑TTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAAt > 2:720366/1‑141 (MQ=255) atCAATGTAATAACAGTTGCGAGTCGATAGGCCCAACTGAATCTGAATGTGCTAAACATG‑TTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCAtt > 3:215949/1‑141 (MQ=255) aTGTAATAACAGTTGCGAGTCGATAGGCCCAACTGAATCTGAATGTGCTAAACATG‑TTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTa < 1:820481/141‑1 (MQ=255) tGTAATAACAGTTGCGAGTCGATAGGCCCAACTGAATCTGAATGTGCTAAACATG‑TTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTaa > 2:405496/1‑141 (MQ=255) aCAGTTGCGAGTCGATAGGCCCAACTGAATCTGAATGTGCTAAACATG‑TTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAgt > 4:217793/1‑141 (MQ=255) cAGTTGCGAGTCGATAGGCCCAACTGAATCTGAATGTGCTAAACATG‑TTAGCAAATGATATCAGTACAAGTGCAATAGACACGGGATATATTAATGTCAACAATGGTACAGCAATTTTTAAAATCATTTCTAAACCAAgtg > 1:639091/1‑141 (MQ=255) gCGAGTCGATAGGCCCAACTGAATCTGAATGTGCTAAACATG‑TTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTa > 4:332172/1‑141 (MQ=255) gCGAGTCGATAGGCCCAACTGAATCTGAATGTGCTAAACATG‑TTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTa > 2:255279/1‑141 (MQ=255) gCGAGTCGAGAGGCCCAACTGAATCTGAATGTGCGAAACATG‑TTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTGAAAATCATTTCTAAACCAAGTGTTGTa > 2:919185/1‑141 (MQ=255) cGATAGGCCCAACTGAATCTGAATGTGCTAAACATG‑TTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAg > 4:120751/1‑141 (MQ=255) aGGCCCAACTGAATCTGAATGTGCTAAACATG‑TTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAAcc < 4:53836/141‑1 (MQ=255) cccAACTGAATCTGAATGTGCTAAACATG‑TTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTa < 4:128871/141‑1 (MQ=255) cccAACTGAATCTGAATGTGCTAAACATG‑TTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTa > 1:794655/1‑141 (MQ=255) aCTGAATCTGAATGTGCTAAACATG‑TTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGAt > 2:72150/1‑141 (MQ=255) aatctgaatGTGCTAAACATG‑TTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATagag > 2:1060990/1‑141 (MQ=255) aatctgaatGTGCTAAACATG‑TTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAACAACGGGACAGCAATAAATAAAATCATTTCTACACCAAGTGGTGTAAATAAGAACCATATGATagaa > 2:236542/1‑140 (MQ=255) atctgaatGTGCTAAACATG‑TTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAgaga > 2:528439/1‑141 (MQ=255) tctgaatGTGCTAAACATG‑TTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGaa > 1:361677/1‑141 (MQ=255) ctgaatGTGCTAAACATG‑TTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGaaa < 2:687855/141‑1 (MQ=255) tgaatGTGCTAAACATG‑TTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGaaaa < 4:217235/141‑1 (MQ=255) gaatGTGCTAAACATG‑TTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAAt < 2:710267/141‑1 (MQ=255) tgCTAAACATG‑TTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATTGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTg < 2:685171/141‑1 (MQ=255) tgCTAAACATG‑TTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTg < 1:977092/141‑1 (MQ=255) gctaaCATGTTTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGAAATATTAAAGTGAAGCATGTGACAGCAATTTGTAAAATCATTTCTAAACCAAGTGTGGTAAATAAGAACCCTATGATAGAGAAAATAAGTGc > 4:6268/4‑141 (MQ=255) cTAAACATG‑TTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCg < 4:26266/141‑1 (MQ=255) cTAAACATG‑TTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCg > 1:336284/1‑141 (MQ=255) cTAAACATG‑TTAGCAAATGATATGAGTACAAGTGAAATCAACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGATCCCTATGATAGAGAAAATAAGTGCg < 3:322310/141‑1 (MQ=255) tAAACATG‑TTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGa > 1:1067904/1‑141 (MQ=255) tAAACATG‑TTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGa > 2:385362/1‑141 (MQ=255) aaaCATG‑TTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGaa < 4:309222/141‑1 (MQ=255) aaaCATG‑TTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGaa > 2:79407/1‑141 (MQ=255) aaCATGTTTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTAAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGACACCTACGACCGAGCAACAAAGTGCCaa > 2:110909/1‑141 (MQ=255) aTG‑TTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGTACAGCAATTTTTAAAATTATTTCTAATCCAAATGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATAtt < 4:224730/141‑1 (MQ=255) tG‑TTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATAttt > 2:669568/1‑141 (MQ=255) g‑TTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATAtttt < 1:383581/141‑1 (MQ=255) ttAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTta > 3:167635/1‑141 (MQ=255) tAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTtat < 2:328949/141‑1 (MQ=255) tAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTtat > 2:10020/1‑141 (MQ=255) aGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTtata > 1:402840/1‑141 (MQ=255) gCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATaa < 1:684463/141‑1 (MQ=255) gCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATaa > 1:1039951/1‑141 (MQ=255) aaTGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGaa > 1:369497/1‑141 (MQ=255) tGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGaaaa > 1:174952/1‑141 (MQ=255) gATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGaaacc < 1:452052/141‑3 (MQ=255) gATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAAc > 1:429890/1‑141 (MQ=255) atGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTa > 2:568226/1‑141 (MQ=255) gAGTTCAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAgg < 1:513752/141‑1 (MQ=255) gAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAgg < 2:550527/141‑1 (MQ=255) gAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATAGTTTATAAGAAAACTTAgg < 2:655398/141‑1 (MQ=255) aCAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACg < 4:13621/141‑1 (MQ=255) cAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACgt < 2:443845/141‑1 (MQ=255) cAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACgt > 3:291831/1‑141 (MQ=255) cAAGTGCAATCGACACGGCATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGCACCCTCTGATAGAGAAAATAAGTGCGACTATTTTATACGAAAACTTAGGTACgt < 2:918369/141‑1 (MQ=255) aaGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACgtg < 1:746782/141‑1 (MQ=255) tGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAgg < 1:158910/132‑1 (MQ=255) tGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAgg > 2:158910/1‑132 (MQ=255) aTCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCttc > 3:15055/1‑140 (MQ=255) aTCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTa > 1:477268/1‑141 (MQ=255) cGACACGGGATATATTAAAGTCAATAATGGGACGGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGt > 1:563338/1‑141 (MQ=255) cacGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTaaa < 2:509280/141‑1 (MQ=255) cacGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTaaa > 1:734716/1‑141 (MQ=255) cGGGATATATTAATGTCAACAATGGTACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAAtg > 2:474763/1‑141 (MQ=255) cGGGATATATTAAATTTGTTAATGGTACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAAtg < 1:332395/141‑1 (MQ=255) cGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAAtg > 1:403016/1‑141 (MQ=255) gggATATATTAAAGTCAATAATGGGACAGCAATTTTTTTTTTTTTTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAAtgt > 2:638176/1‑141 (MQ=255) gggATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAAtgt > 3:28494/1‑141 (MQ=255) gggATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAAtgt > 3:246943/1‑141 (MQ=255) gggATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAAtgt > 2:797055/1‑141 (MQ=255) ggATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAAtgtg > 1:273157/1‑141 (MQ=255) atatTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGgcgc < 2:780795/141‑1 (MQ=255) atatTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGgcgc > 1:178336/1‑141 (MQ=255) atTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCaa > 1:840078/1‑141 (MQ=255) tAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGc > 1:655332/1‑141 (MQ=255) aGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCAtt < 3:405855/141‑1 (MQ=255) tCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGa < 2:1070468/141‑1 (MQ=255) cAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGAc < 2:361677/141‑1 (MQ=255) aataatGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGACg > 3:291349/1‑141 (MQ=255) aataatGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGACg < 3:245641/141‑1 (MQ=255) aacaatGGTACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGACg > 3:133286/4‑141 (MQ=255) ataatGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGACGa > 3:180361/1‑141 (MQ=255) taatGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGt > 2:338240/1‑76 (MQ=255) aatGGTACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGACGAGt < 2:987414/141‑1 (MQ=255) aatGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGACGAGt < 2:1020328/141‑1 (MQ=255) aatGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGACGAGt < 3:223930/141‑1 (MQ=255) atGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGGACGTGTTTCTTAGTAAATGTGGCGCAACCATTGACGAGTc > 4:168143/1‑141 (MQ=255) tGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGACGAGTcc > 1:766155/1‑141 (MQ=255) gACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGACGAGTCCtat < 1:720366/141‑1 (MQ=255) | AGTATTTGTAAAATTGAAATAATCAATGTAATAACAGTTGCGAGTCGATAGGCCCAACTGAATCTGAATGTGCTAAACATG‑TTAGCAAATGATATGAGTACAAGTGCAATCGACACGGGATATATTAAAGTCAATAATGGGACAGCAATTTTTAAAATCATTTCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGACGAGTCCTAT > USA300TCH1516_ALE/355552‑355832 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |