Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 355,854 G→T 5.8% I282I (ATC→ATA brnQ_2 ← Branched‑chain amino acid transport system 2 carrier protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE355,8540GT 5.8% 459.9 / 10.3 137I282I (ATC→ATAbrnQ_2Branched‑chain amino acid transport system 2 carrier protein
Reads supporting (aligned to +/- strand):  ref base G (60/68);  new base T (2/7);  total (62/75)
Fisher's exact test for biased strand distribution p-value = 1.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 138 total aligned reads displayed.

TCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGACGAGTCCTATACATGTTGTTAGGCATGCAAGGATAACCGTCATTCCAAATACGAGGTTACCGAACGAACCAAATAATCGTAATGAGTTGTACGTCAATATATCTGTACCATCTTTAAAGTTTCCTGGAGCTGTTGATGCCCCAACGTATGCAAGTGCAAAGTAAATCAT  >  USA300TCH1516_ALE/355714‑355991
                                                                                                                                            |                                                                                                                                         
  tAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGACGAGTCCTATACATGTTGTTAGGCATGCAAGGAt                                                                                                                                         <  2:825038/141‑1 (MQ=255)
  tAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGACGAGTCCTATACATGTTGTTAGGCATGCAAGGAt                                                                                                                                         >  1:693888/1‑141 (MQ=255)
  tAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGACGAGTCCTATACATGTTGTTAGGCATGCAAGGAt                                                                                                                                         >  4:286768/1‑141 (MQ=255)
  tAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGACGAGTCCTATACATGTTGTTAGGCATGCAAGGAt                                                                                                                                         <  3:146503/141‑1 (MQ=255)
     aCCAAGTGTCGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGACGAGTCCTATACATGTTGTTAGGCATGCAAGGATAAc                                                                                                                                      >  1:954961/1‑141 (MQ=255)
       cAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGACGAGTCCTATACATGTTGTTAGGCATGCAAGGATAACCg                                                                                                                                    <  1:679196/141‑1 (MQ=255)
       cAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGACGAGTCCTATACATGTTGTTAGGCATGCAAGGATAACCg                                                                                                                                    >  4:176551/1‑141 (MQ=255)
        aaGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGACGAGTCCTATACATGTTGTTAGGCATGCAAGGATAACCGt                                                                                                                                   <  3:312433/141‑1 (MQ=255)
        aaGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGACGAGTCCTATACATGTTGTTAGGCATGCAAGGATAACCGt                                                                                                                                   <  1:417820/141‑1 (MQ=255)
        aaGTATTGTAAATAAGTACCGTATGAGAGAGAAAATGAGTGCGCATATTTAATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGACGAGTCCTATACATGTTGTTAGGCATGCAAGGATAACCGt                                                                                                                                   <  1:391399/141‑1 (MQ=255)
         aGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGACGAGTCCTATACATGTTGTTAGGCATGCAAGGATAACCGTc                                                                                                                                  >  3:296956/1‑141 (MQ=255)
          gtgtTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGACGAGTCCTATACATGTTGTTAGGCATGCAAGGATAACCGTCa                                                                                                                                 >  3:57259/1‑141 (MQ=255)
          gtgtTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGACGAGTCCTATACATGTTGTTAGGCATGCAAGGATAACCGTCa                                                                                                                                 >  1:489242/1‑141 (MQ=255)
           tgttgtAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGACGAGTCCTATACATGTTGTTAGGCATGCAAGGATAACCGTCAt                                                                                                                                >  1:516104/1‑141 (MQ=255)
            gttgtAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGACGAGTCCTATACATGTTGTTAGGCATGCAAGGATAACCGTCAtt                                                                                                                               <  1:560922/141‑1 (MQ=255)
            gttgtAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGACGAGTCCTATACATGTTGTTAGGCATGCAAGGATAACCGTCAtt                                                                                                                               <  3:116401/141‑1 (MQ=255)
            gttgtAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGACGAGTCCTATACATGTTGTTAGGCATGCAAGGATAACCGTCAtt                                                                                                                               <  4:24696/141‑1 (MQ=255)
              tgtAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGACGAGTCCTATACATGTTGTTAGGCATGCAAGGATAACCGTCATTcc                                                                                                                             <  1:682946/141‑1 (MQ=255)
               gtAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGACGAGTCCTATACATGTTGTTAGGCATGCAAGGATAACCGTCATTCCa                                                                                                                            >  3:343592/1‑141 (MQ=255)
               gtAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGACGAGTCCTATACATGTTGTTAGGCATGCAAGGATAACCGTCATTCCa                                                                                                                            <  1:797055/141‑1 (MQ=255)
               gtAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGACGAGTCCTATACATGTTGTTAGGCATGCAAGGATAACCGTCATTCCa                                                                                                                            <  1:671369/141‑1 (MQ=255)
                  aaTAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGACGAGTCCTATACATGTTGTTAGGCATGCAAGGATAACCGTCATTCCAAAt                                                                                                                         >  1:82833/1‑141 (MQ=255)
                   aTAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGACGAGTCCTATACATGTTGTTAGGCATGCAAGGATAACCGTCATTCCAAATa                                                                                                                        <  1:189338/141‑1 (MQ=255)
                     aaGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGACGAGTCCTATACATGTTGTTAGGCATGCAAGGATAACCGTCATTCCAAATACg                                                                                                                      <  3:181429/141‑1 (MQ=255)
                       gAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGACGAGTCCTATACATGTTGTTAGGCATGCAAGGATAACCGTCATTCCAAATACGAg                                                                                                                    <  2:840078/141‑1 (MQ=255)
                       gAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGACGAGTCCTATACATGTTGTTAAACATGCAAGTATCACTGTCATACCAAATActat                                                                                                                    <  2:639091/141‑4 (MQ=255)
                        aaCCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGACGAGTCCTATACATGTTGTTAGGCATGCAAGGATAACCGTCATTCCAAATACGAgg                                                                                                                   <  2:267031/141‑1 (MQ=255)
                        aaCCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGACGAGTCCTATACATGTTGTTAGGCATGCAAGGATAACCGTCATTCCAAATACGAgg                                                                                                                   <  2:228766/141‑1 (MQ=255)
                         aCCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGACGAGTCCTATACATGTTGTTAGGCATGCAAGGATAACCGTCATTCCAAATACGAGGt                                                                                                                  >  2:959096/1‑141 (MQ=255)
                           ccTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGACGAGTCCTATACATGTTGTTAGGCATGCAAGGATAACCGTCATTCCAAATACGAGGTTa                                                                                                                <  1:299052/141‑1 (MQ=255)
                           ccTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGACGAGTCCTATACATGTTGTTAGGCATGCAAGGATAACCGTCATTCCAAATACGAGGTTa                                                                                                                >  2:945223/1‑141 (MQ=255)
                           ccTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGACGAGTCCTATACATGTTGTTAGGCATGCAAGGATAAACGTCATTCCAAATACGAGGTTa                                                                                                                >  1:657557/1‑141 (MQ=255)
                              aTGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGACGAGTCCTATACATCTTGTTAGGCATGCAAGGATAACCGTCATTCCCAATACGAGGGTACcg                                                                                                             >  2:910517/1‑141 (MQ=255)
                                  tAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGACGAGTCCTATACATGTTGTTAGGCATGCAAGGATAACCGTCATTCCAAATACGAGGTTACcgaacg                                                                                                         <  2:560916/141‑1 (MQ=255)
                                     agaAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGACGAGTCCTATACATGTTGTTAGGCATGCAAGGATAACCGTCATTCCAAATACGAGGTTACCgaacgaac                                                                                                      <  2:711785/141‑1 (MQ=255)
                                     agaAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGACGAGTCCTATACATGTTGTTAGGCATGCAAGGATAACCGTCATTCCAAATACGAGGTTACCgaacgaac                                                                                                      <  2:522827/141‑1 (MQ=255)
                                      gaAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGACGAGTCCTATACATGTTGTTAGGCGTGCAAGGATAACCGTCATTCCAAATACGAGGTTACCGAACGAAcc                                                                                                     <  2:848398/141‑1 (MQ=255)
                                      gaAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGACGAGTCCTATACATGTTGTTAGGCATGCAAGGATAACCGTCATTCCAAATACGAGGTTACCGTACGCAcc                                                                                                     >  1:803280/1‑141 (MQ=255)
                                      gaAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGACGAGTCCTATACATGTTGTTAGGCATGCAAGGATAACCGTCATTCCAAATACGAGGTTACCGAACGAAcc                                                                                                     >  1:895615/1‑141 (MQ=255)
                                         aaTAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGACGAGTCCTATACATGTTGTTAGGCATGCAAGGATAACCGTCATTCCAAATACGAGGTTACCGAACGAACCaaa                                                                                                  >  1:1086276/1‑141 (MQ=255)
                                          aTAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGACGAGTCCTATACATGTTGTTAGGCATGCAAGGATAACCGTCATTCCAAATACGAGGTTACCGAACGAACCAaat                                                                                                 <  2:490285/141‑1 (MQ=255)
                                                 cGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGACGAGTCCTATACATGTTGTTAGGCATGCAAGGATAACCGTCATTCCAAATACGAGGTTACCGAACGAACCAAATAATCGTa                                                                                          <  1:935735/141‑1 (MQ=255)
                                                       ttttATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGACGAGTCCTATACATGTTGTTAGGCATGCAAGGATAACCGTCATTCCAAATACGAGGTTACCGAACGAACCAAATAATCGTAATGAGt                                                                                    <  4:291831/141‑1 (MQ=255)
                                                         ttATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGACGAGTCCTATACATGTTGTTAGGCATGCAAGGATAACCGTCATTCCAAATACGAGGTTACCGAACGAACCAAATAATCGTAATGAGTTg                                                                                  <  1:1065161/141‑1 (MQ=255)
                                                          tataAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGACGAGTCCTATACATGTTGTTAGGCATGCAAGGATAACCGTCATTCCAAATACGAGGTTACCGAACGAACCAAATAATCGTAATGAGTTGt                                                                                 <  2:234664/141‑1 (MQ=255)
                                                           ataAGAAAACTTAGGTAAGTGTTTCTTAGTAAATGTGGCGCAAGCATTGACGAGTCCTATACATGTTGTTAGGCATGCAAGGATAACCGTCATTCCAAATACGAGGTTACCGAACGAACCAAATAATCGTAATGAGATGTa                                                                                >  4:431134/1‑141 (MQ=255)
                                                             aaGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGACGAGTCCTATACATGTTGTTAGGCATGCAAGGATAACCGTCATTCCAAATACGAGGTTACCGAACGAACCAAATAATCGTAATGAGTTGTACg                                                                              <  2:339816/141‑1 (MQ=255)
                                                              aGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGACGAGTCCTATACATGTTGTTAGCCATGCAAGGATAACCGTCATTCCAAATACGAGGTTACCGAACGAACCAAATAATCGTAATGAGTTGTACGt                                                                             >  4:55739/1‑141 (MQ=255)
                                                                 aaaCTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGACGAGTCCTATACATGTTGTTAGGCATGCAAGGATAACCGTCATTCCAAATACGAGGTTACCGAACGAACCAAATAATCGTAATGAGCTGTACGTCaa                                                                          >  2:824647/1‑141 (MQ=255)
                                                                      tAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGACGAGTCCTATACATGTTGTTAGGCATGCAAGGATAACCGTCATTCCAAATACGAGGTTACCGAACGAACCAAATAATCGTAATGAGTTGTACGTCAatatat                                                                     <  1:410684/141‑1 (MQ=255)
                                                                      tAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGACGAGTCCTATACATGTTGTTAGGCATGCAAGGATAACCGTCATTCCAAATACGAGGTTACCGAACGAACCAAATAATCGTAATGAGTTGTACGTCAatatat                                                                     >  2:255631/1‑141 (MQ=255)
                                                                       aGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGACGAGTCCTATACATGTTGTTAGGCATGCAAGGATAACCGTCATTCCAAATACGAGGTTACCGAACGAACCAAATAATCGTAATGAGTTGTACGTCAATATATc                                                                    <  4:274576/141‑1 (MQ=255)
                                                                        ggTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGACGAGTCCTATACATGTTGTTAGGCATGCAAGGATAACCGTCATTCCAAATACGAGGTTACCGAACGAACCAAATAATCGTAATGAGTTGTACGTCAATATATCt                                                                   <  2:178336/141‑1 (MQ=255)
                                                                        ggTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGACGAGTCCTATACATGTTGTTAGGCATGCAAGGATAACCGTCATTCCAAATACGAGGTTACCGAACGAACCAAATAATCGTAATGAGTTGTACGTCAATATATCt                                                                   >  2:530736/1‑141 (MQ=255)
                                                                        ggTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGACGAGTCCTATACATGTTGTTAGGCATGCAAGGATAACCGTCATTCCAAATACGAGGTTACCGAACGAACCAAATAATCGTAATGAGTTGTACGTCAATATATCt                                                                   <  2:369497/141‑1 (MQ=255)
                                                                             gtgtTTCTTAGTAAATGTGGCGCAAGCATTGACGAGTCCTATACATGTTGTTAGGCATGCAAGGATAACCGTCATTCCAAATACGAGGTTACCGAACGAACCAAATAATCGTAATGAGTTGTACGTCAATATATCTGTAcc                                                              >  3:247353/1‑141 (MQ=255)
                                                                             gtgtTTCTTAGTAAATGTGGCGCAAGCATTGACGAGTCCTATACATGTTGTTAGGCATGCAAGGATAACCGTCATTCCAAATACGAGGTTACCGAACGAACCAAATAATCGTAATGAGTTGTACGTCAATATATCTGTAcc                                                              <  3:286768/141‑1 (MQ=255)
                                                                             gtgtTTCTTAGTAAATGTGGCGCAAGCATTGACGAGTCCTATACATGTTGTTAGGCATGCAAGGATAACCGTCATTCCAAATACGAGGTTACCGAACGAACCAAATAATCGTAATGAGTTGTACGTCAATATATCTGTAcc                                                              <  4:30638/141‑1 (MQ=255)
                                                                                tttCTTAGTAAATGTGGCGCAAGCATTGACGAGTCCTATACATGTTGTTAGGCATGCAAGGATAACCGTCATTCCAAATACGAGGTTACCGAACGAACCAAATAATCGTAATGAGTTGTACGTCAATATATCTGTACCATc                                                           >  2:320550/1‑141 (MQ=255)
                                                                                    ttAGTAAATGTGGCGCAAGCATTGACGAGTCCTATACATGTTGTTAGGCATGCAAGGATAACCGTCATTCCAAATACGAGGTTACCGAACGAACCAAATAATCGTAATGAGTTGTACGTCAATATATCTGTACCATCTTTa                                                       <  4:57259/141‑1 (MQ=255)
                                                                                    ttAGTAAATGTGGCGCAAGCATTGACGAGTCCTATACATGTTGTTAGGCATGCAAGGATAACCGTCATTCCAAATACGAGGTTACCGAACGAACCAAATAATCGTAATGAGTTGTACGTCAATATATCTGTACCATCTTTa                                                       <  4:260422/141‑1 (MQ=255)
                                                                                    ttAGTAAATGTGGCGCAAGCATTGACGAGTCCTATACATGTTGTTAGGCATGCAAGGATAACCGTCATTCCAAATACGAGGTTACCGAACGAACCAAATAATCGTAATGAGTTGTACGTCAATATATCTGTACCATCTTTa                                                       <  2:402840/141‑1 (MQ=255)
                                                                                     tAGTAAATGTGGCGCAAGCATTGACGAGTCCTATACATGTTGTTAGGCATGCAAGGATAACCGTCATTCCAAATACGAGGTTACCGAACGAACCAAATAATCGTAATGAGTTGTACGTCAATATATCTGTACCATCTTTaa                                                      <  3:253024/141‑1 (MQ=255)
                                                                                     tAGTAAATGTGGCGAAAGCATTGACGAGTCCTATACATGTTGTTAGGCATGCAAGGATAACCGTCATTCCAAATACGAGGTTACCGAACGAACCAAATAATCGTAATGAGTTGTACGTCAATATATCTGTACCATCTTTaa                                                      <  1:236542/141‑1 (MQ=255)
                                                                                       gTAAATGTGGCGCAAGCATTGACGAGTCCTATACATGTTGTTAGGCATGCAAGGATAACCGTCATTCCAAATACGAGGTTACCGAACGAACCAAATAATCGTAATGAGTTGTACGTCAATATATCTGTACCATCTTTAAAg                                                    <  1:696072/141‑1 (MQ=255)
                                                                                        tAAATGTGGCGCAAGCATTGACGAGTCCTATACATGTTGTTAGGCATGCAAGGATAACCGTCATTCCAAATACGAGGTTACCGAACGAACCAAATAATCGTAATGAGTTGTACGTCAATATATCTGTACCATCTTTAAAGt                                                   >  2:868270/1‑141 (MQ=255)
                                                                                           aTGTGGCGCAAGCATTGACGAGTCCTATACATGTTGTTAGGCAGTCAAGGATAACCGTCATTCCAAATACGAGGTTACCGAACGAACCAAATAATCGTAATGAGTTGTACGTCAATATATCTGTACCATCTTTAAAGTTTc                                                <  1:10020/141‑1 (MQ=255)
                                                                                            tgtgGCGCAAGCATTGACGAGTCCTATACATGTTGTTAGGCATGCAAGGATAACCGTCATTCCAAATACGAGGTTACCGAACGAACCAAATAATCGTAATGAGTTGTACGTCAATATATCTGTACCATCTTTAAAGTTTcc                                               <  1:528439/141‑1 (MQ=255)
                                                                                            tgtgGCGCAAGCATTGACGAGTCCTATACATGTTGTTAAACATGCAAGTATCACTGTCATACCAAATACTATGTTGCCAAATGAACCAAATACTCGTAATGAGTTGTACGTCAATATATCTGTACCATCTTTAAAGTTTcc                                               <  1:261558/141‑1 (MQ=255)
                                                                                            tgtgGCGCAAGCATTGACGAGTCCTATACATGTTGTTAAACATGCAAGTATCACTGTCATACCAAATACTATGTTGCCAATGAACCAAATACTCGTAATGAGTTGTACGTCAATATATCTGTACCATCTTTAAAGTTTCCt                                              <  1:731989/141‑1 (MQ=255)
                                                                                               ggCGCAAGCATTGACGAGTCCTATACATGTTGTTAGGCATGCAAGGATAACCGTCATTCCAAATACGAGGTTACCGAACGAACCAAATAATCGTAATGAGTTGTACGTCAATATATCTGTACCATCTTTAAAGTTTCCTgg                                            <  2:990235/141‑1 (MQ=255)
                                                                                               ggCGCAAGCATTGACGAGCCCTATACATGTTGTTAGGCATGCAAGGATAACCGCCATTCCAAATACGAGGTTACCGAACGAACCAAATAATCGTAATGAGTTGTACGTCAATATATCTGTACCATCTTTAAAGTTTCCTgg                                            >  2:795191/1‑141 (MQ=255)
                                                                                                gcgcAAGCATTGACGAGTCCTATACATGTTGTTAGGCATGCAAGGATAACCGTCATTCCAAATACGAGGTTACCGAACGAACCAAATAATCGTAATGAGTTGTACGTCAATATATCTGTACCATCTTTAAAGTTTCCTGGa                                           >  2:848064/1‑141 (MQ=255)
                                                                                                 cgcAAGCATTGACGAGTCCTATACATGTTGTTAGGCATGCAAGGATAACCGTCATTCCAAATACGAGGTTACCGAACGAACCAAATAATCGTAATGAGTTGTACGTCAATATATCTGTACCATCTTTAAAGTTTCCTGGAg                                          >  2:995559/1‑141 (MQ=255)
                                                                                                 cgcAAGCATTGACGAGTCCTATACATGTTGTTAGGCATGCAAGGATAACCGTCATTCCAAATACGAGGTTACCGAACGAACCAAATAATCGTAATGAGTTGTACGTCAATATATCTGTACCATCTTTAAAGTTTCCTGGAg                                          <  2:750470/141‑1 (MQ=255)
                                                                                                      gCATTGAAGAGTCCTATACATGTTGTTAGGCATGCAAGGATAACCGTCATTCCAAATACGAGGTTACCGAACGAACCAAATAATCGTAATGAGTTGTACGTCAATATATCTGCACCATCTTTAAAGTTTCCTGGAGCTGtt                                     >  3:402943/1‑141 (MQ=255)
                                                                                                       cATTGACGAGTCCTATACATGTTGTTAGGCATGCAAGGATAACCGTCATTCCAAATACGAGGTTACCGAACGAACCAAATAATCGTAATGAGTTGTACGTCAATATATCTGTACCATCTTTAAAGTTTCCTGGAGCTGTTg                                    <  4:15055/141‑1 (MQ=255)
                                                                                                         ttGACGAGTCCTATACATGTTGTTAGGCATGCAAGGATAACCGTCATTCCAAATACGAGGTTACCGAACGAACCAAATAATCGTAATGAGTTGTACGTCAATATATCTGTAACATCTTTAAAGTTTCCTGGAGCTGTTGAt                                  >  2:55385/1‑141 (MQ=255)
                                                                                                         ttGACGAGTCCTATACATGTTGTTAGGCATGCAAGGATAACCGTCATTCCAAATACGAGGTTACCGAACGAACCAAATAATCGTAATGAGTTGTACGTCAATATATCCGTACCATCTTTAAAGTTTCCTGGAGCTGTTGAt                                  >  2:695732/1‑141 (MQ=255)
                                                                                                            aCGAGTCCTATACATGTTGTTAAACATGCAAGTATCACTGTCATACCAAATACTATGTTGCCAAATGAACCAAATACTCGTAATGAGTTGTACGTCAATATATCTGTACCATCTTTAAAGTTTCCTGGAGCTGTTGATGcc                               <  1:1016404/141‑1 (MQ=255)
                                                                                                                gTCCTATACATGTTGTTAGGCATGCAAGGATAACCGTCATTCCAAATACGAGGTTACCGAACGAACCAAATAATCGTAATGAGTTGTACGTCAATATATCTGTACCATCTTTAAAGTTTCCTGGAGCTGTTGATGCCCCaa                           <  4:180361/141‑1 (MQ=255)
                                                                                                                 tCCTATACATGTTGTTAGGCATGCAAGGATAACCGTCATTCCAAATACGAGGTTACCGAACGAACCAAATAATCGTAATGAGTTGTACGTCAATATATCTGTACCATCTTTAAAGTTTCCTGGAGCTGTTGATGCCCCAAc                          <  2:89429/141‑1 (MQ=255)
                                                                                                                  ccTATACATGTTGTTAGGCATGCAAGGATAACCGTCATTCCAAATACGAGGTTACCGAACGAACCAAATAATCGTAATGAGTTGTACGTCAATATATCTGTACCATCTTTAAAGTTTCCTGGAGCTGTTGATGCCCCAACg                         <  2:750857/141‑1 (MQ=255)
                                                                                                                  ccTATACATGTTGTTAGGCATGCAAGGATAACCGTCATTCCAAATACGAGGTTACCGAACGAACCAAATAATCGTAATGAGTTGTACGTCAATATATCTGTACCATCTTTAAAGTTTCCTGGAGCTGTTGATGCCCCAACg                         >  1:29403/1‑141 (MQ=255)
                                                                                                                            ttgttAGGCATGCAAGGATAACCGTCATTCCAAATACGAGGTTACCGAACGAACCAAATAATCGTAATGAGTTGTACGTCAATATATCTGTACCATCTTTAAAGTTTCCTGGAGCTGTTGATGCCCCAACGTATGCAAGTg               >  3:313458/1‑141 (MQ=255)
                                                                                                                             tgttAGGCATGCAAGGATAACCGTCATTCCAAATACGAGGTTACCGAACGAACCAAATAATCGTAATGAGTTGTACGTCAATATATCTGTACCATCTTTAAAGTTTCCTGGAGCTGTTGATGCCCCAACGTATGCAAGTGc              <  2:420027/141‑1 (MQ=255)
                                                                                                                               ttAGGCATGCAAGGATAACCGTCATTCCAAATACGAGGTTACCGAACGAACCAAATAATCGTAATGAGTTGTACGTCAATATATCTGTACCATCTTTAAAGTTTCCTGGAGCTGTTGATGCCCCAACGTATGCAAGTGCaa            >  3:282036/1‑141 (MQ=255)
                                                                                                                                tAGGCATGCAAGGATAACCGTCATTCCAAATACGAGGTTACCGAACGAACCAAATAATCGTAATGAGTTGTACGTCAATATATCTGTACCATCTTTAAAGTTTCCTGGAGCTGTTGATGCCCCAACGTATGCAAGTGCaaa           <  1:128090/141‑1 (MQ=255)
                                                                                                                                 aGGCATGCAAGGATAACCGTCATTCCAAATACGAGGTTACCGAACGAACCAAATAATCGTAATGAGTTGTACGTCAATATATCTGTACCATCTTTAAAGTTTCCTGGAGCTGTTGATGCCCCAACGTATGCAAGTGCAAAg          >  1:630477/1‑141 (MQ=255)
                                                                                                                                 aGGCATGCAAGGATAACCGTCATTCCAAATACGAGGTTACCGAACGAACCAAATAATCGTAATGAGTTGTACGTCAATATATCTGTACCATCTTTAAAGTTTCCTGGAGCTGTTGATGCCCCAACGTATGCAAGTGCAAAg          <  3:217793/141‑1 (MQ=255)
                                                                                                                                 aGGCATGCAAGGATAACCGTCATTCCAAATACGAGGTTACCGAACGAACCAAATAATCGTAATGAGTTGTACGTCAATATATCTGTACCATCTTTAAAGTTTCCTGGAGCTGTTGATGCCCCAACGTATGCAAGTGCAAAg          >  1:344908/1‑141 (MQ=255)
                                                                                                                                  ggCATGCAAGTATAACCATCATTCCAAATACGTGCTTACCGAACGAACCAAATAATCGTAATGAGTTGTACGTCAATATATCTGTACCATCTTTAAAGTTACCTTGAGCTGTTGATGCCCCAACGTATGCAAGTGCAAAGt         <  4:223419/141‑1 (MQ=255)
                                                                                                                                   gCATGCAAGGATAACCGTCATTCCAAATACGAGGTTACCGAACGAACCAAATAATCGTAATGAGTTGTACGTCAATATATCTGTACCATCTTTAAAGTTTCCTGGAGCTGTTGATGCCCCAACGTATGCAAGTGCAAAGTa        >  1:1021331/1‑141 (MQ=255)
                                                                                                                                   gCATGCAAGGATAACCGTCATTCCAAATACGAGGTTACCGAACGAACCAAATAATCGTAATGAGTTGTACGTCAATATATCTGTACCATCTTTAAAGTTTCCTGGAGCTGTTGATGCCCCAACGTATGCAAGTGCAAAGTa        >  3:413486/1‑141 (MQ=255)
                                                                                                                                   gCATGCAAGGATAACCGTCATTCCAAATACGAGGTTACCGAACGAACCAAATAATCGTAATGAGTTGTACGTCAATATATCTGTACCATCTTTAAAGTTCCCTGGAGCTGTTGATGCCCCAACGTATGCAAGTGCAAAGTa        <  2:655332/141‑1 (MQ=255)
                                                                                                                                     aTGCAAGGATAACCGTCATTCCAAATACGAGGTTACCGAACGAACCAAATAATCGTAATGAGTTGTACGTCAATATATCTGTACCATCTTTAAAGTTTCCTGGAGCTGTTGATGCCCCAACGTATGCAAGTGCAAAGTaaa      <  1:777196/141‑1 (MQ=255)
                                                                                                                                     aTGCAAGGATAACCGTCATTCCAAATACGAGGTTACCGAACGAACCAAATAATCGTAATGAGTTGTACGTCAATATATCTGTACCATCTTTAAAGTTTCCTGGAGCTGTTGATGCCCCAACGTATGCAAGTGCAAAGTaaa      >  2:689651/1‑141 (MQ=255)
                                                                                                                                      tGCAAGGATAACCGTCATTCCAATTACGAGGTTACCGAACGAACCAAATAATCGTAATGAGTTGTACGTCAATATATCTGTACCATCTTTAAAGTTTCCTGGAGCTGTTGATGCCCCAACGTATGCAAGTGCAAAGGAAAt     >  2:674397/1‑141 (MQ=255)
                                                                                                                                        cAAGGATAACCGTCATTCCAAATACGAGGTTACCGAACGAACCAAATAATCGTAATGAGTTGTACGTCAATATATCTGTACCATCTTTAAAGTTTCCTGGAGCTGTTGATGCCCCAACGTATGCAAGTGCAAAGTAAATCa   >  1:364017/1‑141 (MQ=255)
                                                                                                                                         aaGGATAACCGTCATTCCAAATACGAGGTTACCGAACGAACCAAATAATCGTAATGAGTTGTACGTCAATATATCTGTACCATCTTTAAAGTTTCCTGGAGCTGTTGATGCCCCAACGTATGCAAGTGCAAAGTAAATCAt  <  3:442104/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                         
TCTAAACCAAGTGTTGTAAATAAGAACCCTATGATAGAGAAAATAAGTGCGAATATTTTATAAGAAAACTTAGGTACGTGTTTCTTAGTAAATGTGGCGCAAGCATTGACGAGTCCTATACATGTTGTTAGGCATGCAAGGATAACCGTCATTCCAAATACGAGGTTACCGAACGAACCAAATAATCGTAATGAGTTGTACGTCAATATATCTGTACCATCTTTAAAGTTTCCTGGAGCTGTTGATGCCCCAACGTATGCAAGTGCAAAGTAAATCAT  >  USA300TCH1516_ALE/355714‑355991

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: