BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	480,623	G→A	5.5%	K550K (AAG→AAA)	USA300HOU_RS02280 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	480,623	0	G	A	5.5%	522.8 / 10.3	168	K550K (AAG→AAA)	USA300HOU_RS02280	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (76/82); new base A (6/4); total (82/86)
Fisher's exact test for biased strand distribution p-value = 5.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 169 total aligned reads displayed.

ACATTTAAATTGATGAAAAATAATGTCATGCCTAGTCTGTTATTGCCTGAATTAAGTGGGCCATTTTTAAGCTTAAGTACCATTGTCAATTCGATTATGCCTAGAAAAAGTCGCGCGTCTTTACAAAAAATAAAACAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGTGAGTTTGACCGAACATCAGACTTACCTGTTGGATTTACTGAGCAAGAGCAAATTGATTTCGCGTTACAAGCGTTGAAATTGATGGATTTAACCGAAGCA  >  USA300TCH1516_ALE/480483‑480761

acaTTTAAATTGATGAAAAATAATGTCATGCCTAGTCTGTTATTGCCTGAATTAAGTGGGCCATTTTTAAGCTTAAGTACCATTGTCAATTCGATTATGCCTAGAAAAAGTCGCGCGTCTTTACAAAAAATAAAACAAAAg                                                                                                                                            >  4:234990/1‑141 (MQ=255)

   tttAAATTGATGAAAAATAATGTCATGCCTAGTCTGTTATTGCCTGAATTAAGTGGGCCATTTTTAAGCTTAAGTACCATTGTCAATTCGATTATGCCTAGAAAAAGTCGCGCGTCTTTACAAAAAATAAAACAAAAGTgg                                                                                                                                         >  3:318065/1‑141 (MQ=255)

    ttAAATTGATGAAAAATAATGTCATGCCTAGTCTGTTATTGCCTGAATTAAGTGGGCCATTTTTAAGCTTAAGTACCATTGTCAATTCGATTATGCCTAGAAAAAGTCGCGCGTCTTTACAAAAAATAAAACAAAAGTGGt                                                                                                                                        >  3:380904/1‑141 (MQ=255)

     tAAATTGATGAAAAATAATGTCATGCCTAGTCTGTTATTGCCTGAATTAAGTGGGCCATTTTTAAGCTTAAGTACCATTGTCAATTCGATTATGCCTAGAAAAAGTCGCGCGTCTTTACAAAAAATAAAACAAAAGTGGtt                                                                                                                                       >  2:603672/1‑141 (MQ=255)

       aaTTGATGAAAAATAATGTCATGCCTAGTCTGTTATTGCCTGAATTAAGTGGGCCATTTTTAAGCTTAAGTACCATTGTCAATTCGATTATGCCTAGAAAAAGTCGCGCGTCTTTACAAAAAATAAAACAAAAGTGGTTGa                                                                                                                                     <  2:999256/141‑1 (MQ=255)

        aTTTATTAAAAATAATGTCATGCCTAGTCTGTTATTGCCTGAATTAAGTGGGCCATTTTTAAGCTTAAGTACCATTGTCAATTCGATTATGCCTAGAAAAAGTCGCGCGTCTTTACAAAAAATAAAACAAAAGTGGTTGaa                                                                                                                                    <  2:761777/141‑1 (MQ=255)

        aTTGATGAAAAATAATGTCATGCCTAGTCTGTTATTGCCTGAACTAAGTGGGCCATTTTTAAGCTTAAGTACCATTGTCAATTCGATTATGCCTAGAAAAAGTCGCGCGTCTTTACAAAAAATAAAACAAAAGTGGTTGaa                                                                                                                                    <  1:855351/141‑1 (MQ=255)

          tgatgaAAAATAATGTCATGCCTAGTCTGTTATTGCCTGAATTAAGTGGGCCATTTTTAAGCTTAAGTACCATTGTCAATTCGATTATGCCTAGAAAAAGTCGCGCGTCTTTACAAAAAATAAAACAAAAGTGGTTGaaaa                                                                                                                                  <  1:111864/141‑1 (MQ=255)

               aaaaaTAATGTCATGCCTAGTCTGTTATTGCCTGAATTAAGTGGGCCATTTTTAAGCTTAAGTACCATTGTCAATTCGATTATGCCTAGAAAAAGCCGCGCGTCTTTACAAAAAATAAAACAAAAGTGGTTGAAAAAGCCt                                                                                                                             <  4:44495/141‑1 (MQ=255)

                aaaaTAATGTCATGCCTAGTCTGTTATTGCCTGAATTAAGTGGGCCATTTTTAAGCTTAAGTACCATTGTCAATTCGATTATGCCTAGAAAAAGTCGCGCGTCTTTACAAAAAATAAAACAAAAGTGGTTGAAAAAGCCTg                                                                                                                            >  1:888742/1‑141 (MQ=255)

                     aatGTCATGCCTAGTCTGTTATTGCCTGAATTAAGTGGGCCATTTTTAAGCTTTATTATAATTGTCAATTCGATTATGCCTAGAAAAAGTCGCGCGTCTTTACAAAAAATAAAACAAAAGTGGTTGAAAAAGCCTGAAACa                                                                                                                       >  2:322005/1‑141 (MQ=255)

                       tGTCATGCCTAGTCTGTTATTGCCTGAATTAAGTGGGCCATTTTTAAGCTTAAGTACCATTGTCAATTCGATTATGCCTAGAAAAAGTCGCGCGTCTTTACAAAAAATAAAACAAAAGTGGTTGAAAAAGCCTGAAACaaa                                                                                                                     >  1:855322/1‑141 (MQ=255)

                        gTCATGCCTAGTCTGTTATTGCCTGAATTAAGTGGGCCATTTTTAAGCTTAAGTACCATTGTCAATTCGATTATGCCTAGAAAAAGTCGCGCGTCTTTACAAAAAATAAAACAAAAGTGGTTGAAAAAGCCTGAAACAAAg                                                                                                                    <  3:428598/141‑1 (MQ=255)

                        gTCATGCCTAGTCTGTTATTGCCTGAATGAAGTGGGCCATTTTTAAGCTTAAGTACCATTGTCAATTCGATTATGCCTCGAAAAAGTCGCGCGTCTTTACAAAAAATAAAACAAAAGTGGTTGAAAAAGCCTGAAACAAAg                                                                                                                    >  2:576101/1‑141 (MQ=255)

                          cATGCCTAGTCTGTTATTGCCTGAATTAAGTGGGCCATTTTTAAGCTTAAGTACCATTGTCAATTCGATTATGCCTAGAAAAAGTCGCGCGTCTTTACAAAAAATAAAACAAAAGTGGTTGAAAAAGCCTGAAACAAAGtt                                                                                                                  <  3:436474/141‑1 (MQ=255)

                              ccTAGTCTGTTATTGCCTGAATTAAGTGGGCCATTTTTAAGCTTAAGTACCATTGTCAATTCGATTATGCCTAGAAAAAGTCGCGCGTCTTTACAAAAAATAAACCAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACg                                                                                                              <  2:381956/141‑1 (MQ=255)

                               cTAGTCTGTTATTGCCTGAATTAAGTGGGCCATTTTTAAGCTTAAGTACCATTGTCAATTCGATTATGCCTAGAAAAAGTCGCGCGTCTTTACAAAAAATAAAAAAAACATGGTTGAAATGTCCGGAAACAAAGTGGACGa                                                                                                             >  4:300596/1‑141 (MQ=255)

                                tAGTCTGTTATTGCCTGAATTAAGTGGGCCATTTTTAAGCTTGAGTACCATTGTCAATTCGATTATGCCTAGAAAAAGTCGCGCGTCTTTACAAAAAATAAAACAAAAATGGTTGAAAAAGCCTGAAACAAAGTTAACGAt                                                                                                            <  1:52106/141‑1 (MQ=255)

                                 aGTCTGTTATTGCCTGAATTAAGTGGGCCATTTTTTTTTTTTTTTACCATTGTCAATTCGATTATGCCTAGAAAAAGTCGCGCGTCTTTACAACAAATATACCACAAATGGTTGAAAAATCCTGAAACAAAGttaccgctt                                                                                                           >  4:84576/1‑134 (MQ=255)

                                       ttATTGCCTGACTTGAGTGGTCCATTTTTAAGCTTAAGAACCATTGTCAATACGATTATGCCTAGAAAAAGTCGCACGTCAAAACAAAAAATAAAACAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGt                                                                                                     <  4:34802/141‑1 (MQ=255)

                                       ttATTGCCTGAATTAAGTGGGCCATTTTTAAGCTTAAGTACCATTGTCAATTCGATTATGCCTAGAAAAAGTCGCGCGTCTTTACAAAAAATAAAACAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGt                                                                                                     <  1:992801/141‑1 (MQ=255)

                                       ttATTGCCTGAATTAAGTGGGCCATTTTTAAGCTTAAGTACCATTGTCAATTCGATTATGCCTAGAAAAAGTCGCGCGTCTTTACAAAAAATAAAACAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGt                                                                                                     <  1:926495/141‑1 (MQ=255)

                                        tATTGCCTGAATTAAGTGGGCCATTTTTAAGCTTAAGTACCATTGTCAATTCGATTATGCCTAGAAAAAGTCGCGCGTCTTTACAAAAAATAAAACAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGTg                                                                                                    <  1:424420/141‑1 (MQ=255)

                                         aTTGCCTGAATTAAGTGGGCCATTTTTAAGCTTAAGTACCATTGTCAATTCGATTATGCCTAGAAAAAGTCGCGCGTCTTTACAAAAAATAAAACAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGTGa                                                                                                   <  2:718246/141‑1 (MQ=255)

                                             ccTGAATTAAGTGGGCCATTTTTAAGCTTAAGTACCATTGTCAATTCGATTATGCCTAGAAAAAGTCGCGCGTCTTTACAAAAAATAAAACAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGTGAGttt                                                                                               >  1:365155/1‑141 (MQ=255)

                                              cTGAATTAAGTGGGCCATTTTTAAGCTTGAGTACCATTGTCAATTCGATTATGCCTAGAAAAAGTCGCGCGTCTTTACAAAAAATAAAACAAAAATGGTTGAAAAAGCCTGAAACAAAGTTAACGATTGATCGTGAGTTTg                                                                                              <  2:42138/141‑1 (MQ=255)

                                              cTGAATTAAGTGGGCCATTTTTAAGCTTAAGTACCATTGTCAATTCGATTATGCCTAGAAAAAGTCGCGCGTCTTTACAAAAAATAAAACAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGTGAGTTTg                                                                                              >  2:275319/1‑141 (MQ=255)

                                                gAATTAAGTGGGCCATTTTTAAGCTTGAGTACCATTGTCAATTCGATTATGCCTAGAAAAAGTCGCGCGTCTTTACAAAAAATAAAACAAAAATGGTTGAGAAAGCCTGAAACAAAGTTAACGATTGATCGTGAGTTTGAc                                                                                            <  3:258125/141‑1 (MQ=255)

                                                gAATTAAGTGGGCCATTTTTAAGCTTAAGTACCATTGTCAATTCGATTATGCCTAGAAAAAGTCGCGCGTCTTTACAAAAAATAAAACAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGTGAGTTTGAc                                                                                            >  1:151360/1‑141 (MQ=255)

                                                gAATTAAGTGGGCCATTTTTAAGCTTAAGTACCATTGTCAATTCGATTATGCCTAGAAAAAGTCGCGCGTCTTTACAAAAAATAAAACAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGTGAGTTTGAc                                                                                            >  1:799007/1‑141 (MQ=255)

                                                  aTTAAGTGACCCATATATAAGCTAAAGTACCATCGTCAATACGAAAAAGCCAAGAAAAAGTCGAGCGTCTATAAAAAAAATAAAACAAAAGAGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGTGAGTTTGACCg                                                                                          <  2:1045403/141‑1 (MQ=255)

                                                       gTGGGCCATTTTTAAGCTTAAGTACCCTTGTCAATGCGATTATGCCTAGAAAAAGTCGCGCGTCTTTACAAAAAATAAAACAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGTGAGTTTGACCGAACAt                                                                                     >  4:225640/1‑141 (MQ=255)

                                                       gTGGGCCATTTTTAAGCTTAAGTACCATTGTCAATTCGATTATGCCTAGAAAAAGTCGCGCGTCTTTACAAAAAATAAAACAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGTGAGTTTGACCGAACAt                                                                                     <  1:210696/141‑1 (MQ=255)

                                                        tGGGCCATTTTTAAGCTTAAGTACCATTGTCAATTCGATTATGCCTAGAAAAAGTCGCGCGTCTTTACAAAAAATAAAACAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGTGAGTTTGACCGAACATc                                                                                    <  4:252662/141‑1 (MQ=255)

                                                           gCCATTTTTAAGCTTAAGTACCATTGTCAATTCGATTATGCCTAGAAAAAGTCGCGCGTCTTTACAAAAAATAAAACAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGTGAGTTTGACCGAACATCAGa                                                                                 >  1:104360/1‑141 (MQ=255)

                                                            ccATTTTTAAGCTTAAGTACCATTGTCAATTCGATTATGCCTAGAAAAAGTCGCGCGTCTTTACAAAAAATAAAACAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGTGAGTTTGACCGAACATCAGAc                                                                                >  1:32065/1‑141 (MQ=255)

                                                            ccATTTTGAAGCTTGAGTACCATTGTCAATTCGATTATGCCTAGAAAAAGTCGCGCGTCTTTACAAAAAATAAAACAAAAATGGTTGAAAAAGCCTGAAACAAAGTTAACGATTGATCGTGAGTTTGACCGAACATCAGAc                                                                                >  2:209218/1‑141 (MQ=255)

                                                             cATTTTTAAGCTTAAGTACCATTGTCAATTCGATTATGCCTAGAAAAAGTCGCGCGTCTTTACAAAAAATAAAACAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGTGAGTTTGACCGAACATCAGACt                                                                               <  2:511374/141‑1 (MQ=255)

                                                               tttttAAGCTTAAGTACCATTGTCAATTCGATTATGCCTAGAAAAAGTCGCGCGTCTTTACAAAAAATAAAACAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGTGAGTTTGACCGAACATCAGACTTa                                                                             >  1:946707/1‑141 (MQ=255)

                                                               tttttAAGCTTAAGTACCATTGTCAATTCGATTATGCCTAGAAAAAGTCGCGCGTCTTTACAAAAAATAAAACAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGTGAGTTTGACCGAACATCAGACTTa                                                                             >  1:385741/1‑141 (MQ=255)

                                                                   tAAGCTTAAGTACCATTGTCAATTCGATTATGCCTAGAAAAAGTCGCGCGTCTTTACAAAAAATAAAACAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGTGAGTTTGACCGAACATCAGACTTACCTg                                                                         <  3:72131/141‑1 (MQ=255)

                                                                    aaGCTTAAGTACCATTGTCAATTCGATTATGCCTAGAAAAAGTCGCGCGTCTTTACAAAAAATAAAACAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGTGAGTTTGACCGAACATCAGACTTACCTGt                                                                        <  1:275319/141‑1 (MQ=255)

                                                                    aaGCTTAAGTACCATTGTCAATTCGATTATGCCTAGAAAAAGTCGCGCGTCTTTACAAAAAATAAAACAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGTGAGTTTGACCGAACATCAGACTTACCTGt                                                                        >  2:361567/1‑141 (MQ=255)

                                                                     aGCTTAAGTACCATTGTCAATTCGATTATGCCTAGAAAAAGTCGCGCGTCTTTACAAAAAATAAAACAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGTGAGTTTGACCGAACATCAGACTTACCTGtt                                                                       >  3:367779/1‑141 (MQ=255)

                                                                       cTTAAGTACCATTGTCAATTCGATTATGCCTAGAAAAAGTCGCGCGTCTTTACAAAAAATAAAACAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGTGAGTTTGACCGAACATCAGACTTACCTGTTgg                                                                     <  3:129822/141‑1 (MQ=255)

                                                                       cTTAAGTACCATTGTCAATTCGATTATGCCTAGAAAAAGTCGCGCGTCTTTACAAAAAATAAAACAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGTGAGTTTGACCGAACATCAGACTTACCTGTTgg                                                                     <  2:476723/141‑1 (MQ=255)

                                                                       cTTAAGTACCATTGTCAATTCGATTATGCCTAGAAAAAGTCGCGCGTCTTTACAAAAAATAAAACAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGTGAGTTTGACCGAACATCAGACTTACCTGTTgg                                                                     <  1:352044/141‑1 (MQ=255)

                                                                       cTTAAGTACCATTGTCAATTCGATTATGCCTAGAAAAAGTCGCGCGTCTTTACAAAAAATAAAACAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGTGAGTTTGACCGAACATCAGACTTACCTGTTgg                                                                     <  2:839750/141‑1 (MQ=255)

                                                                         tAAGTACCATTGTCAATTCGATTATGCCTAGAAAAAGTCGCGCGTCTTTACAAAAAATAAAACAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGTGAGTTTGACCGAACATCAGACTTACCTGTTGGAt                                                                   <  1:916046/141‑1 (MQ=255)

                                                                         tAAGTACCATTGTCAATTCGATTATGCCTAGAAAAAGTCGCGCGTCTTTACAAAAAATAAAACAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGTGAGTTCGACCGAACATCAGACTTACCTGTTGGAt                                                                   <  2:291199/141‑1 (MQ=255)

                                                                             tACCATTGTCAATTCGATTATGCCTAGAAAAAGTCGCGCGTCTTTACAAAAAATAAAACAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGTGAGTTTGACCGAACATCAGACTTACCTGTTGGATTTAc                                                               <  1:713683/141‑1 (MQ=255)

                                                                             tACCATTGTCAATTCGATTATGCCTAGAAAAAGTCGCGCGTCTTTACAAAAAATAAAACAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGTGAGTTTGACCGAACATCAGACTTACCTGTTGGATTTAc                                                               <  3:119381/141‑1 (MQ=255)

                                                                               ccATTGTCAATTCGATTATGCCTAGAAAAAGTCGCGCGTCTTTACAAAAAATAAAACAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGTGAGTTTGACCGAACATCAGACTTACCTGTTGGATTTACTg                                                             >  2:1003883/1‑141 (MQ=255)

                                                                               ccATTGTCAATTCGATTATGCCTAGAAAAAGTCGCGCGTCTTTACAAAAAATAAAACAAAAATGGTTGAAAAAGCCTGAAACAAAGTTAACGATTGATCGTGCGTTTGACGGAACATCAGACTTACCTGTTGGATTTACTg                                                             >  1:41898/1‑141 (MQ=255)

                                                                                cATTGTCAATTCGATTATGCCTAGAAAAAGTCGCGCGTCTTTACAAAAAATAAAACAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGTGAGTTTGACCGAACATCAGACTTACCTGTTGGATTTACTga                                                            >  2:949250/1‑141 (MQ=255)

                                                                                 aTTGTCAATTCGATTATGCCTAGAAAAAGTCGCGCGTCTTTACAAAAAATAAAACAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGTGAGTTTGACCGAACATCAGACTTACCTGTTGGATTTACTgag                                                           <  2:403085/141‑1 (MQ=255)

                                                                                 aTTGTCAATTCGATTATGCCTAGAAAAAGTCGCGCGTCTTTACAAAAAATAAAACAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGTGAGTTTGACCGAACATCAGACTTACCTGTTGGATTTACTgag                                                           >  2:1073903/1‑141 (MQ=255)

                                                                                 aTTGTCAATTCGATTATGCCTAGAAAAAGTCGCGCGTCTTTACAAAAAATAAAACAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGTGAGTTTGACCGAACATCAGACTTACCTGTTGGATTTACTgag                                                           >  2:696218/1‑141 (MQ=255)

                                                                                   tGTCAATTCGATTATGCCTAGAAAAAGTCGCGCGTCTTTACAAAAAATAAAACAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGTGAGTTTGACCGAACATCAGACTTACCTGTTGGATTTACTgagca                                                         >  2:721321/1‑141 (MQ=255)

                                                                                   tGTCAATTCGATTATGCCTAGAAAAAGTCGCGCGTCTTTACAAAAAATAAAACAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGTGAGTTTGACCGAACATCAGACTTACCTGTTGGATTTACTgagca                                                         <  1:487268/141‑1 (MQ=255)

                                                                                        aTTCGATTATGCCTAGAAAAAGTCGCGCGTCTTTACAAAAAATAAAACAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGTGAGTTTGACCGAACATCAGACTTACCTGTTGGATTTACTgagcaagagc                                                    >  1:1044263/1‑141 (MQ=255)

                                                                                           cGATTATGCCTAGAAAAAGTCGCGCGTCTTTACAAAAAATAAAACAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGTGAGTTTGACCGAACATCAGACTTACCTGTTGGATTTACTGAGCAAGAGCaaa                                                 >  2:463726/1‑141 (MQ=255)

                                                                                                aTGCCTAGAAAAAGTCGCGCGTCTTTACAAAAAATAAAACAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGTGAGTTTGACCGAACATCAGACTGACCTATTGGATTTACTGAGCAAGAGCAAATTGAt                                            >  1:1005212/1‑141 (MQ=255)

                                                                                                      aGAAAAAGTCGCGCGTCTTTACAAAAAATAAAACAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGTGAGTTTGACCGAACATCAGACTTACCTGTTGGATTTACTGAGCAAGAGCAAATTGATTTcgcg                                      <  2:799007/141‑1 (MQ=255)

                                                                                                      aGAAAAAGTCGCGCGTCTTTACAAAAAATAAAACAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGTGAGTTTGACCGAACATCAGACTTACCTGTTGGATTTACTGAGCAAGAGCAAATTGATTTcgcg                                      >  4:382116/1‑141 (MQ=255)

                                                                                                      aGAAAAAGTCGCGCGTCTGTACAAAAAATAAAACAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGTGAGTTTGACCGAACATCAGACTTACCTGTTGGATTTACTGAGCAAGAGCAAATTGATTTcgcg                                      <  1:748662/141‑1 (MQ=255)

                                                                                                        aaaaaGTCGCGCGTCTTTACAAAAAATAAAACAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGTGAGTTTGACCGAACATCAGACTTACCTGTTGGATTTACTGAGCAAGAGCAAATTGATTTCGCGtt                                    <  1:178502/141‑1 (MQ=255)

                                                                                                        aaaaaGTCGCGCGTCTTTACAAAAAATAAAACAAAAATGGTTGAAAAAGCCTGAAACAAAGTTAACGATTGATCGTGAGTTTGACCGAACATCAGACTTACCTGTTGGATTTACTGAGCACGAGCAAATTGATTTCGCGtt                                    >  3:368195/1‑141 (MQ=255)

                                                                                                         aaaaGTCGCGCGTCTTTACAAAAAATAAAACAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGTGAGTTTGACCGAACATCAGACTTACCTGTTGGATTTACTGAGCAAGAGCAAATTGATTTCGCGTTa                                   <  4:437740/141‑1 (MQ=255)

                                                                                                            aatCGCGTGTCTTTACAAAAAATAAAACAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGTGAGTTTGACCGAACATCAGACTTACCTGTTGGATTTACTGAGCAAGAGCAAATTGATTTCGCGTTACaa                                <  3:30628/139‑1 (MQ=255)

                                                                                                            aGTCGAGCGTCTGTACCGGAAATAAAAGAACTGTATTTGGAAAACCCTGAGGCAAAGTTGACGAGTGGTCGGGAGTTTGACCGAACATCAGACTTACCTGTTGGATTTACTGAGCAAGGGCAATTTGATTTCGCGTTACaa                                <  2:311875/141‑1 (MQ=255)

                                                                                                              tCGCGCGTCTTTACAAAAAATAAAACAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGTGAGTTTGACCGAACATCAGACTTACCTGTTGGATTTACTGAGCAAGAGCAAATTGATTTCGCGTTACAAGc                              >  1:1000714/1‑141 (MQ=255)

                                                                                                              tCGCGCGTCTTTACAAAAAATAAAACAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGTGAGTTTGACCGAACATCAGACTTACCTGTTGGATTTACTGAGCAAGAGCAAATTGATTTCGCGTTACAAGc                              >  4:369211/1‑141 (MQ=255)

                                                                                                                gcgctTCTTTACAAAAAATAAAACAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGTGAGTTTGACCGAACATCAGACTTACCTGTTGGATTTACTGAGCAAGAGCAAATTGATTTCGCGTTACAAGCGt                            <  2:1005537/141‑1 (MQ=255)

                                                                                                                gcgcgTCTTTACAAAAAATAAAACAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGTGAGTTTGACCGAACATCAGACTTACCTGTTGGATTTACTGAGCAAGAGCAAATTGATTTCGCGTTACAAGCGt                            <  2:735144/141‑1 (MQ=255)

                                                                                                                 cgcgTCTTTACAAAAAATAAAACAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGTGAGTTTGACCGAACATCAGACTTACCTGTTGGATTTACTGAGCAAGAGCAAATTGATTTCGCGTTACAAGCGtt                           <  1:1011378/141‑1 (MQ=255)

                                                                                                                 cgcgTCTTTACAAAAAATAAAACAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGTGAGTTTGACCGAACATCAGACTTACCTGTTGGATTTACTGAGCAAGAGCAAATTGATTTCGCGTTACAAGCGtt                           >  4:208884/1‑141 (MQ=255)

                                                                                                                 cgcgTCTTTACAAAAAATAAAACAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGTGAGTTTGACCGAACATCAGACTTACCTGTTGGATTTACTGAGCAAGAGCAAATTGATTTCGCGTTACAAGCGtt                           <  1:769880/141‑1 (MQ=255)

                                                                                                                 cgcgACTTTACAAAAAATAAAACAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGTGAGTTTGACCGAACATCAGACTTACCTGTTGGATTTACTGAGCAAGAGCAAATTGATTTCGCGTTACAAGCGtt                           <  2:607999/141‑1 (MQ=255)

                                                                                                                  gcgTCTTTACAAAAAATAAAACAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGTGAGTTTGACCGAACATCAGACTTACCTGTTGGATTTACTGAGCAAGAGCAAATTGATTTCGCGTTACAAGCGTTg                          <  2:892457/141‑1 (MQ=255)

                                                                                                                  gcgTCTTTACAAAAAATAAAACAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGTGAGTTTGACCGAACATCAGACTTACCTGTTGGATTTACTGAGCAAGAGCAAATTGATTTCGCGTTACAAGCGTTg                          >  3:247683/1‑141 (MQ=255)

                                                                                                                     tCTTTACAAAAAATAAAACAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGTGAGTTTGACCGAACATCAGACTTACCTGTTGGATTTACTGAGCAAGAGCAAATTGATTTCGCGTTACAAGCGTTGaaa                       <  1:739788/141‑1 (MQ=255)

                                                                                                                       tttACAAAAAATAAAACAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGTGAGTTTGACCGAACATCAGACTTACCCGTTGGCTATAATTAACAAGAACTAATAGTTTTCACGTTACAAGCGTAGAAAtt                     >  1:209545/1‑141 (MQ=255)

                                                                                                                         tACAAAAAATAAAACAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGTGAGTTTGACCGAACATCAGACTTACCTGTTGGATTTACTGAGCAAGAGCAAATTGATTTCGCGTTACAAGCGTTGAAATTGa                   >  2:867891/1‑141 (MQ=255)

                                                                                                                         tACAAAAAATAAAACAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGTGAGTTTGACCGAACATCAGACTTACCTGTTGGATTTACTGAGCAAGAGCAAATTGATTTCGCGTTACAAGCGTTGAAATTGa                   <  3:173317/141‑1 (MQ=255)

                                                                                                                          aCAAAAAATAAAACAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGTGAGTTTGACCGAACATCAGACTTACCTGTTGGATTTACTGAGCAAGAGCAAATTGATTTCGCGTTACAAGCGTTGAAATTGAt                  <  3:328990/141‑1 (MQ=255)

                                                                                                                          aCAAAAAATAAAACAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGTGAGTTTGACCGAACATCAGACTTACCTGTTGGATTTACTGAGCAAGAGCAAATTGATTTCGCGTTACAAGCGTTGAAATTGAt                  <  2:179491/141‑1 (MQ=255)

                                                                                                                              aaaaTAAAACAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGTGAGTTTGACCGAACATCAGACTTACCTGTTGGATTTACTGAGCAAGAGCAAATTGATTTCGCGTTACAAGCGTTGAAATTGATGGAt              <  2:225529/141‑1 (MQ=255)

                                                                                                                                aaTAAAACAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGTGAGTTTGACCGAACATCAGACTTACCTGTTGGATTTACTGAGCAAGAGCAAATTGATTTCGCGTTACAAGCGTTGAAATTGATGGAttt            <  4:88893/141‑1 (MQ=255)

                                                                                                                                aaTAAAACAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGTGAGTTTGACCGAACATCAGACTTACCTGTTGGATTTACTGAGCAAGAGCAAATTGATTTCGCGTTACAAGCGTTGAAATTGATGGAttt            >  1:152833/1‑141 (MQ=255)

                                                                                                                                aaTAAAACAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGTGAGTTTGACCGAACATCAGACTTACCTGTTGGATTTACTGAGCAAGAGCAAATTGATTTCGCGTTACAAGCGTTGAAATTGATGGAttt            >  4:218176/1‑141 (MQ=255)

                                                                                                                                aaTAAAACAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGTGAGTTTGACCGAACATCAGACTTACCTGTTGGATTTACTGAGCAAGAGCAAATTGATTTCGCGTTACAAGCGTTGAAATTGATGGAttt            >  1:530314/1‑141 (MQ=255)

                                                                                                                                    aaaCAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACGAGTGATCGTGAGTTTGACCGAACATCAGACTTACCTGTTGGATTTACTGAGCAAGAGCAAATTGATTTCGCGTTACAAGCGTTGAAATTGATGGATTTAAcc        >  4:138406/1‑141 (MQ=255)

                                                                                                                                     aaCAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGTGAGTTTGACCGAACATCAGACTTACCTGTTGGATTTACTGAGCAAGAGCAAATTGATTTCGCGTTACAAGCGTTGAAATTGATGGATTTAACCg       >  2:241008/1‑141 (MQ=255)

                                                                                                                                     aaCAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGTGAGTTTGACCGAACATCAGACTTACCTGTTGGATTTACTGAGCAAGAGCAAATTGATTTCGCGTTACAAGCGTTGAAATTGATGGATTTAACCg       >  1:961286/1‑141 (MQ=255)

                                                                                                                                     aaCAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGTGAGTTTGACCGAACATCAGACTTACCTGTTGGATTTACTGAGCAAGAGCAAATTGATTTCGCGTTACAAGCGTTGAAATTGATGGATTTAACCg       >  3:350334/1‑141 (MQ=255)

                                                                                                                                       cAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGTGAGTTTGACCGAACATCAGACTTACCTGTTGGATTTACTGAGCAAGAGCAAATTGATTTCGCGTTACAAGCGTTGAAATTGATGGATTTAACCGaa     <  1:464129/141‑1 (MQ=255)

                                                                                                                                        aaaaGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGTGAGTTTGACCGAACATCAGACTTACCTGTTGGATTTACTGAGCAAGAGCAAATTGATTTCGCGTTACAAGCGTTGAAATTGATGGATTTAACCGAAg    <  1:449804/141‑1 (MQ=255)

                                                                                                                                         aaaGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGTGAGTTTGACCGAACATCAGACTTACCTGTTGGATTTACTGAGCAAGAGCAAATTGATTTCGCGTTACAAGCGTTGAAATTGATGGATTTAACCGAAGc   >  1:547800/1‑141 (MQ=255)

                                                                                                                                         aaaGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGTGAGTTTGACCGAACATCAGACTTACCTGTTGGATTTACTGAGCAAGAGCAAATTGATTTCGCGTTACAAGCGTTGAAATTGATGGATTTAACCGAAGc   <  1:965332/141‑1 (MQ=255)

ACATTTAAATTGATGAAAAATAATGTCATGCCTAGTCTGTTATTGCCTGAATTAAGTGGGCCATTTTTAAGCTTAAGTACCATTGTCAATTCGATTATGCCTAGAAAAAGTCGCGCGTCTTTACAAAAAATAAAACAAAAGTGGTTGAAAAAGCCTGAAACAAAGTTGACGATTGATCGTGAGTTTGACCGAACATCAGACTTACCTGTTGGATTTACTGAGCAAGAGCAAATTGATTTCGCGTTACAAGCGTTGAAATTGATGGATTTAACCGAAGCA  >  USA300TCH1516_ALE/480483‑480761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`