Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 491,332 T→A 5.6% intergenic (+90/‑242) metQ_1 → / → sle1_1 putative D‑methionine‑binding lipoprotein MetQ/N‑acetylmuramoyl‑L‑alanine amidase sle1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE491,3320TA 5.6% 430.6 / 11.7 138intergenic (+90/‑242)metQ_1/sle1_1putative D‑methionine‑binding lipoprotein MetQ/N‑acetylmuramoyl‑L‑alanine amidase sle1
Reads supporting (aligned to +/- strand):  ref base T (63/66);  new base A (5/3);  total (68/70)
Fisher's exact test for biased strand distribution p-value = 4.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 143 total aligned reads displayed.

AATTTATCTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAATTATAAAATTTGATGATACAGTATATGATTTTTTTGTAATCATAATGTCATCA  >  USA300TCH1516_ALE/491192‑491472
                                                                                                                                            |                                                                                                                                            
aaTTTATCTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGAt                                                                                                                                              <  4:123906/141‑1 (MQ=255)
      tCTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAAt                                                                                                                                        <  2:940976/141‑1 (MQ=255)
        tAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCa                                                                                                                                      <  1:1065523/141‑1 (MQ=255)
              tGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTTGACAATAAAATTATTTCCAATAGAGAAAGGGAATGTAATCATTTtat                                                                                                                                <  1:593423/141‑1 (MQ=255)
              tGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTtat                                                                                                                                <  2:905027/141‑1 (MQ=255)
               gagaTTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTtata                                                                                                                               >  1:518105/1‑141 (MQ=255)
                agaTTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTTGACAATAAAATTATTTCCAATAGAGAAAGGGAATGTAATCATTTTATAg                                                                                                                              >  2:718791/1‑141 (MQ=255)
                 gaTTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGt                                                                                                                             >  2:763428/1‑141 (MQ=255)
                 gaTTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGt                                                                                                                             >  2:280327/1‑141 (MQ=255)
                   ttAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTCGGGCGTTATTGTTGGAGACTAAAATTCTTTCCAATAGAGAAGGGGATTGTAATCATTTTATAGTGa                                                                                                                           <  1:973687/141‑1 (MQ=255)
                   ttAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGa                                                                                                                           >  4:166125/1‑141 (MQ=255)
                   ttAAAGAAATAGAAACGTCATTAGCAAAATAAATAATATTGCATACAACAAGCAAAGTTCATGCTTATGTTAGTCGGAAGATAATGTTGGAGCCTACAATTATTATCAATCCAGAAAGGGACTTAAATCATTTTATAGTGa                                                                                                                           <  1:973900/141‑1 (MQ=255)
                       ggCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTACATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTGTTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAata                                                                                                                       >  3:16494/1‑141 (MQ=255)
                        gCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAatat                                                                                                                      >  2:891724/1‑141 (MQ=255)
                          aaTAGAAACGTCATGAGGAAAATAAATTATATGGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAAtatta                                                                                                                    <  2:31613/141‑1 (MQ=255)
                            tAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATg                                                                                                                  <  1:283128/141‑1 (MQ=255)
                              gAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTTGACAATAAAATTATTTCCAATAGAGAAAGGGAATGTAATCATTTTATAGTGAAATATTATGaa                                                                                                                >  1:1020180/1‑141 (MQ=255)
                                 aCGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAAtt                                                                                                             >  2:757262/1‑141 (MQ=255)
                                 aCGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAAtt                                                                                                             >  1:188079/1‑141 (MQ=255)
                                  cGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTg                                                                                                            <  1:325299/141‑1 (MQ=255)
                                  cGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTg                                                                                                            <  4:7789/141‑1 (MQ=255)
                                   gTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGt                                                                                                           <  3:224920/141‑1 (MQ=255)
                                    tCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGta                                                                                                          <  1:2717/141‑1 (MQ=255)
                                    tCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGta                                                                                                          <  1:2711/141‑1 (MQ=255)
                                     cATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGtaa                                                                                                         <  3:32101/141‑1 (MQ=255)
                                     cATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGtaa                                                                                                         >  1:124287/1‑141 (MQ=255)
                                         aGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTaataat                                                                                                     <  2:169965/141‑1 (MQ=255)
                                         aGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAAGTGTaataat                                                                                                     >  2:376722/1‑141 (MQ=255)
                                         aGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATCTTTGTAGGGCGTTATTGTTGGATAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTaataat                                                                                                     <  1:375912/141‑1 (MQ=255)
                                              aataaatTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGa                                                                                                >  2:409367/1‑141 (MQ=255)
                                                taaatTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTTGACAATAAAATTATTTCCAATAGAGAAAGGGAATGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGata                                                                                              >  1:306152/1‑141 (MQ=255)
                                                taaatTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGata                                                                                              >  2:1041924/1‑141 (MQ=255)
                                                 aaatTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGatat                                                                                             <  3:752/141‑1 (MQ=255)
                                                   atTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTg                                                                                           <  3:131545/141‑1 (MQ=255)
                                                   atTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTg                                                                                           >  2:733269/1‑141 (MQ=255)
                                                   atTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTg                                                                                           <  1:48734/141‑1 (MQ=255)
                                                    ttATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGt                                                                                          >  1:991631/1‑141 (MQ=255)
                                                    ttATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGt                                                                                          >  1:573272/1‑141 (MQ=255)
                                                    ttATATTGCGGCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGAGTTATTGTTGGAGAAGAAAATTATTTCAAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGt                                                                                          <  4:214202/141‑1 (MQ=255)
                                                      atatTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTaa                                                                                        <  3:131689/141‑1 (MQ=255)
                                                       tatTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTaaa                                                                                       <  1:251540/141‑1 (MQ=255)
                                                        atTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTaaaa                                                                                      >  4:136575/1‑141 (MQ=255)
                                                          tGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATc                                                                                    >  3:454555/1‑141 (MQ=255)
                                                          tGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATc                                                                                    <  1:269087/141‑1 (MQ=255)
                                                             gTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCtaa                                                                                 >  1:66578/1‑141 (MQ=255)
                                                             gTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCtaa                                                                                 >  3:358635/1‑141 (MQ=255)
                                                             gTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCtaa                                                                                 >  1:862810/1‑141 (MQ=255)
                                                                       cAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGtaa                                                                       <  2:737375/141‑1 (MQ=255)
                                                                         tatgtCATACTTATGTATGGATTGGGTAATTGTAGAAAAATAAAATTATTTCAAATAGAGAAAGAGATTGTAATCATTTTATAGTTAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGtaata                                                                     <  2:409072/137‑1 (MQ=255)
                                                                         aaGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGtaata                                                                     <  2:1082117/141‑1 (MQ=255)
                                                                         aaGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGtaata                                                                     >  1:878433/1‑141 (MQ=255)
                                                                         aaGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGtaata                                                                     >  4:268835/1‑141 (MQ=255)
                                                                         aaGTTCATGCTTATGTTTGTAGGGCGTCATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATAGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGtaata                                                                     >  2:979254/1‑141 (MQ=255)
                                                                          aGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGtaataa                                                                    <  2:469343/141‑1 (MQ=255)
                                                                           gTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTaataat                                                                   >  1:717143/1‑141 (MQ=255)
                                                                             tCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAAttt                                                                 <  2:71694/141‑1 (MQ=255)
                                                                              cATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAAtttt                                                                >  1:906408/1‑141 (MQ=255)
                                                                              cATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAAtttt                                                                >  1:638415/1‑141 (MQ=255)
                                                                               aTGCTTATGTTTGTAGGGCGTTATTGTTTGACAAAATATTTATTTCCAATAGAGAAAGGGTATGTAATCATGTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTa                                                               <  2:300226/141‑1 (MQ=255)
                                                                               aTGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTTGATATTGTAAAATCTAATAAGTTGTAATAATTTTa                                                               >  2:518177/1‑141 (MQ=255)
                                                                               aTGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTa                                                               >  1:764522/1‑141 (MQ=255)
                                                                                 gCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATTGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAg                                                             >  2:618124/1‑141 (MQ=255)
                                                                                 gCTTATGTTTGTAGGGCGTTATTGTCGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAg                                                             >  2:918170/1‑141 (MQ=255)
                                                                                    tATGGTTGTAGGGCGTTATTGTTGGAGAATAAAGTTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAgggg                                                          >  4:123541/1‑141 (MQ=255)
                                                                                      tGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTa                                                        >  2:1069518/1‑141 (MQ=255)
                                                                                      tGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTa                                                        <  2:331611/141‑1 (MQ=255)
                                                                                      tGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTa                                                        >  4:403266/1‑141 (MQ=255)
                                                                                        tttGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAAt                                                      <  1:1033491/141‑1 (MQ=255)
                                                                                        tttGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAAt                                                      >  1:853895/1‑141 (MQ=255)
                                                                                         ttGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAAtt                                                     <  3:208044/141‑1 (MQ=255)
                                                                                         ttGTAGGGCGTTATTGTTGAAGAATAAAATTATTTCCAATAGAGAAAGGGTTTGTAATCATATTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATATTTTTAAGGGGTAAtt                                                     <  4:53108/141‑1 (MQ=255)
                                                                                          tGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAATta                                                    >  1:44937/1‑141 (MQ=255)
                                                                                           gTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAATtat                                                   <  4:373457/141‑1 (MQ=255)
                                                                                           gTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAATtat                                                   <  1:445895/141‑1 (MQ=255)
                                                                                            tAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAATtata                                                  >  2:960458/1‑141 (MQ=255)
                                                                                             aGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAATTATaa                                                 >  1:783673/1‑141 (MQ=255)
                                                                                                  gTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAATTATAAAAttt                                            <  3:221314/141‑1 (MQ=255)
                                                                                                     aTTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAATTATAAAATTtgat                                         <  2:629270/141‑1 (MQ=255)
                                                                                                                 tAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAATTATAAAATTTGATGATACAGTatat                             <  1:281118/141‑1 (MQ=255)
                                                                                                                 tAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAATTATAAAATTTGATGATACAGTatat                             <  2:1081697/141‑1 (MQ=255)
                                                                                                                  aaaaTTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAATTATAAAATTTGATGATACAGTATATg                            >  1:540629/1‑141 (MQ=255)
                                                                                                                  aaaaTTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAATTATAAAATTTGATGATACAGTATATg                            >  3:269189/1‑141 (MQ=255)
                                                                                                                     attattTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAGATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAATTATAAAATTTGATGATACAGTATATGAtt                         >  1:819681/1‑141 (MQ=255)
                                                                                                                       tattTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATCGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAATTATAAAATTTGATGATACAGTATATGAttgt                       <  2:862810/141‑3 (MQ=255)
                                                                                                                         tttCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAATTATAAAATTTGATGATACAGTATATGAtttttt                     <  2:217087/141‑1 (MQ=255)
                                                                                                                          ttCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAATTATAAAATTTGATGATACAGTATATGAttttttt                    >  1:808882/1‑141 (MQ=255)
                                                                                                                           tCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAATTATAAAATTTGATGATACAGTATATGATTTTTTTg                   <  1:358131/141‑1 (MQ=255)
                                                                                                                            ccAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAATTATAAAATTTGATGATACAGTATATGATTTTTTTGt                  <  2:198967/141‑1 (MQ=255)
                                                                                                                             cAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAATTATAAAATTTGATGATACAGTATATGATTTTTTTGTa                 >  2:835759/1‑141 (MQ=255)
                                                                                                                             cAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAACTTTAAGGGGTAATTATAAAATTTGATGATACAGTATATGAtttttttttt                 >  4:167907/1‑138 (MQ=255)
                                                                                                                             cAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTATGATATGGTAAAATCTAATAAGTTGTAATAATTTTAAGGGATAATTATAAAATTTGATGATACAGTATATGATTTTTTTGTa                 >  2:865130/1‑141 (MQ=255)
                                                                                                                                  gagaAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAATTATAAAATTTGATGATACAGTATATGATTTTTTTGTAATCAt            >  2:427806/1‑141 (MQ=255)
                                                                                                                                  gagaAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAATTATAAAATTTGATGATACAGTATATGATTTTTTTGTAATCAt            >  1:408067/1‑141 (MQ=255)
                                                                                                                                  gagaAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAATTATAAAATTTGATGATACAGTATATGATTTTTTTGTAATCAt            <  1:146533/141‑1 (MQ=255)
                                                                                                                                    gaAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAATTATAAAATTTGATGATACAGTATATGATTTTTTTGTAATCATaa          <  3:298253/141‑1 (MQ=255)
                                                                                                                                     aaaGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAATTATAAAATTTGATGATACAGTATATGATTTTTTTGTAATCATAAt         <  2:496483/141‑1 (MQ=255)
                                                                                                                                       aGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAATTATAAAATTTGATGATACAGTATATGATTTTTTTGTAATCATAATGt       >  2:986882/1‑141 (MQ=255)
                                                                                                                                        gggATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAATTATAAAATTTGATGATACAGTATATGATTTTTTTGTAATCATAATGtc      <  2:385671/141‑1 (MQ=255)
                                                                                                                                            ttGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAATTATAAAATTTGATGATACAGTATATGATTTTTTTGTAATCATAATGtcatca  >  3:375618/1‑141 (MQ=255)
                                                                                                                                            ttGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAATTATAAAATTTGATGATACAGTATATGATTTTTTTGTAATCATAATGtcatca  <  3:184458/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                            
AATTTATCTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAATTATAAAATTTGATGATACAGTATATGATTTTTTTGTAATCATAATGTCATCA  >  USA300TCH1516_ALE/491192‑491472

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: