BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	495,185	T→C	5.9%	L309L (TTA→TTG)	USA300HOU_RS02365 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	495,185	0	T	C	5.9%	387.9 / 9.8	131	L309L (TTA→TTG)	USA300HOU_RS02365	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (59/64); new base C (4/4); total (63/68)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 133 total aligned reads displayed.

ATTGTGATAGGAATTGTAAGTACGATACCTATACCACCTGTTAGTGCGCGCGCAATTTCTAACGACCAATTCATCGAAATAGTATAAGTCACTGTATTGGCATTTTTTAAAAAGATTAAAAACATAGGTAGTGCACCGGATAAATATGAGAATAATAAGATGTTAGTCATTGTTCCCATAATATCTTGGCCGATGTTTCGCCCAGCAAGCGCCCATCTCCTCATTGAAATGTGTGGCGTACGCTGTAAAATTTCATGCATACCACTAGCAATTGTAATTGC  >  USA300TCH1516_ALE/495045‑495325

atTGTGATAGGAATTGTAAGTACGATACCTATACCACCTGTTAGTGCGCGCGCAATTTCTAACGACCAATTCATCGAAATAGTATAAGTCACTGTATTGGCATTTTTTAAAAAGATTAAAAACATAGGTAGTGCACCGGAt                                                                                                                                              >  3:434336/1‑141 (MQ=255)

 ttGTGATAGGAATTGTAAGTACGATACCTATACCACCTGTTAGTGCGAGCGCAGTTTATAGTCTTCATTTCTGTGGTACAGAACATGTTACTGTATTGTCATTTTTTAAATAGATTAAAAACATAGGTAGTGCACCGGATa                                                                                                                                             >  1:102257/1‑141 (MQ=255)

  tgtgATAGGAATTGTAAGTACGATACCTATACCACCTGTTAGTGCGCGCGCAATTTCTAACGACCAATTCATCGAAATAGTATAAGTCACTGTATTGGCATTTTTTAAAAAGATTAAAAACATAGGTAGTGCACCGGATaa                                                                                                                                            >  3:430935/1‑141 (MQ=255)

  tgtgATAGGAATTGTAAGTACGATACCTATACCACCTGTTAGTGCGCGCGCAATTTCTAACGACCAATTCATCGAAATAGTATAAGTCACTGTATTGGCATTTTTTAAAAAGATTAAAAACATAGGTAGTGCACCGGATaa                                                                                                                                            >  1:769835/1‑141 (MQ=255)

    tgATAGGAATTGTAAGTACGATACCTATACCACCTGTTAGTGCGCGCGCAATTTCTAACGACCAATTCATCGAAATAGTATAAGTCACTGTATTGGCATTTTTTTTATATATTTAAAACATAGTTAGTGCACCGGATAAat                                                                                                                                          >  4:84190/1‑141 (MQ=255)

    tgATAGGAATTGTAAGTACGATACCTATACCACCTGTTAGTGCGCGCGCAATTTCTAACGACCAATTCATCGAAATAGTATAAGTCACTGTATTGGCATTTTTTAAAAATATTAAAAACATAGGTAGTGCACCGGATAAat                                                                                                                                          <  2:238671/141‑1 (MQ=255)

    tgATAGGAATTGTAAGTACGATACCTATACCACCTGTTAGTGCGCGCGCAATTTCTAACGACCAATTCATCGAAATAGTATAAGTCACTGTATTGGCATTTTTTAAAAAGATTAAAAACATAGGTAGTGCACCGGATAAat                                                                                                                                          >  4:84340/1‑141 (MQ=255)

    tgATAGGAATTGTAAGTACGATACCTATACCACCTGTTAGTGCGCGCGCAATTTCTAACGACCAATTCATCGAAATAGTATAAGTCACTGTATTGGCATTTTTTAAAAAGATTAAAAACATAGGTAGTGCACCGGATAAat                                                                                                                                          >  1:674028/1‑141 (MQ=255)

     gATAGGAATTGTAAGTACGATACCTATACCACCTGTTAGTGCGCGCGCAATTTCTAACGACCAATTCATCGAAATAGTATAAGTCACTGTATTGGCATTTTTTAAAAAGATTAAAAACATAGGTAGTGCACCGGATAAata                                                                                                                                         <  2:880913/141‑1 (MQ=255)

     aaTAGGAATTGTACGTCCGCTACCTATACCACCTGTTAGTGCGCGCGCAATTTCTAACGACCAATTCATCGAAATAGTATAAGTCACTGTATTGGCATTTTTTAAAAAGATTAAAAACATAGGTAGTGCACCGGATAAata                                                                                                                                         <  4:244694/140‑1 (MQ=255)

        aGGAATTGTAAGTACGATACCTATACCACCTGTTAGTGCGCGCGCAATTTCTAACGACCAATTCATCGAAATAGTATAAGTCACTGTATTGGCATTTTTTAAAAAGATTAAAAACATAGGTAGTGCACCGGATAAATATga                                                                                                                                      >  1:409043/1‑141 (MQ=255)

        aGGAATTGTAAGTACGATACCTATACCACCTGTTAGTGCGCGCGCAACTTCTAACGACCAATTCATCGAAATAGTATAAGTCACTGTATTGGCATTTTTTAAAAAGATTAAAAACATAGGTAGTGCACCGGATAAATATga                                                                                                                                      <  3:244196/141‑1 (MQ=255)

         ggAATTGTAAGTACGATACCTATACCACCTGTTAGTGCGCGCGCAATTTCTAACGACCAATTCATCGAAATAGTATAAGTCACTGTATTGGCATTTTTTAAAAAGATTAAAAACATAGGTAGTGCACCGGATAAATATgag                                                                                                                                     <  1:912243/141‑1 (MQ=255)

             ttGTAAGTACGATACCTATACCGCCTGTTAGTGCGCGCGCAATTTCTAACGACCAATTCATCGAAATAGTATAAGTCACAGTATTTGCATTTTTTAAAAAGATTAAAAACATAGGTAGTGCACCGGACAAATATGAGaata                                                                                                                                 >  1:546655/1‑141 (MQ=255)

               gTAAGTACGATACCTATACCGCCTGTTAGTGCGCGCGCAATTTCTAACGACCAATTCATCGAAATAGTATAAGTCACAGTATTTGCATTTTTTAAAAAGATTAAAAACATAGGTAGTGCACCGGACAAATATGAGaataat                                                                                                                               >  4:132179/1‑141 (MQ=255)

                     aCGATACCTATACCACCTGTTAGTGCGCGCGCAATTTCTAACGACCAATTCATCGAAATAGTATAAGTCACTGTATTGGCATTTTTTAAAAAGATTAAAAACATAGGTAGTGCACCGGATAAATATGAGAATAATAAGATg                                                                                                                         <  1:779105/141‑1 (MQ=255)

                     aCGATACCTATACCACCTGTTAGTGCGCGCGCAATTTCTAACGACCAATTCATCGAAATAGTATAAGTCACTGTATTGGCATTTTTTAAAAAGATTAAAAACATAGGTAGTGCACCGGATAAATATGAGAATAATAAGATg                                                                                                                         <  1:1053957/141‑1 (MQ=255)

                      cGATACCTATACCACCTGTTAGTGCGCGCGCAATTTCTAACGACCAATTCATCGAAATAGTATAAGTCACTGTATTGGCATTTTTTAAAAAGATTAAAAACATAGGTAGTGCACCGGATAAATATGAGAATAATAAGATGt                                                                                                                        >  2:793675/1‑141 (MQ=255)

                         tACCTATACCACCTGTTAGTGCGCGCGCAATTTCTAACGACCAATTCATCGAAATAGTATAAGTCACTGTATTGGCATTTTTTAAAAAGATTAAAAACATAGGTAGTGCACCGGATAAATATGAGAATAATAAGATGTTAg                                                                                                                     <  1:276276/141‑1 (MQ=255)

                          aCCTATACCACCTGTTAGTGCGCGCGCAATTTCTAACGACCAATTCATCGAAATAGTATAAGTCACTGTATTGGCATTTTTTAAAAAGATTAAAAACATAGGTAGTGCACCGGATAAATATGAGAATAATAAGATGTTAGt                                                                                                                    <  2:696128/141‑1 (MQ=255)

                            cTATACCACCTGTTAGTGCGCGCGCAATTTCTAACGACCAATTCATCGAAATAGTATAAGTCACTGTATTGGCATTTTTTAAAAAGATTAAAAACATAGGTAGTGCACCGGATAAATATGAGAATAATAAGATGTTAGTCa                                                                                                                  <  1:419613/141‑1 (MQ=255)

                            cTATACCACCTGTTAGTGCGCGCGCAATTTCTAACGACCAATTCATCGAAATAGTATAAGTCACTGTATTGGCATTTTTTAAAAAGATTAAAAACATAGGTAGTGCACCGGATAAATATGAGAATAATAAGATGTTAGTCa                                                                                                                  >  2:174680/1‑141 (MQ=255)

                              ataCCACCTGTTAGTGCGCGCGCAATTTCTAACGACCAATTCATCGAAATAGTATAAGTCACTGTATTGGCATTTTTTAAAAAGATTAAAAACATAGGTAGTGCACCGGATAAATATGAGAATAATAAGATGTTAGTCAtt                                                                                                                <  1:925849/141‑1 (MQ=255)

                              ataCCACATGTGAGTGCGCGCGCAATTTCTAACGACCAATTCATCGAAATAGTATAAGTCACTGTATTGGCATTTTTTAAAAAGATTAAAAACATAGGTAGTGCACCGGATAAATATGAGAATAATAAGATGTTAGTCAtt                                                                                                                <  1:389005/141‑1 (MQ=255)

                               taCCGCCTGTTAGTGCGCGCGCAATTTCTAACGACCAATTCATCGAAATAGTATAAGTCACAGTATTTGCATTTTTTAAAAAGATTAAAAACATAGGTAGTGCACCGGACAAATATGAGAATAATAAGATGTTAGTCATTg                                                                                                               <  3:186238/141‑1 (MQ=255)

                                accaccTGTTAGTGCGCGCGCAATTTCTAACGACCAATTCATCGAAATAGTATAAGTCACTGTATTGGCATTTTTTTTAATTTTTTTAAACATAGGTAGTGCGACGGATAAATATGAGAATAATAAGATGTTAGTCATTGt                                                                                                              >  2:174459/1‑141 (MQ=255)

                                accaccTGTTAGTGCGCGCGCAATTTCTAACGACCAATTCATCGAAATAGTATAAGTCACTGTATTGGCATTTTTTAAAAAGATTAAAAACATAGGTAGTGCACCGGATAAATATGAGAATAATAAGATGTTAGTCATTGt                                                                                                              >  1:364558/1‑141 (MQ=255)

                                 ccaccTGTTAGTGCGCGCGCAATTTCTAACGACCAATTCATCGAAATAGTATAAGTCACTGTATTGGCATTTTTTAAAAAGATTAAAAACATAGGTAGTGCACCGGATAAATATGAGAATAATAAGATGTTAGTCATTGtt                                                                                                             <  1:509677/141‑1 (MQ=255)

                                   accTGTTAGTGCGCGCGCAATTTCTAACGACCAATTCATCGAAATAGTATAAGTCACTGTATTGGCATTTTTTAAAAAGATTAAAAACATAGGTAGTGCACCGGATAAATATGAGAATAATAAGATGTTAGTCATTGTTcc                                                                                                           <  2:865875/141‑1 (MQ=255)

                                     cTGTTAGTGCGCGCGCAATTTCTAACGACCAATTCATCGAAATAGTATAAGTCACTGTATTGGCATTTTTTAAAAAGATTAAAAACATAGGTAGTGCACCGGATAAATATGAGAATAATAAGATGTTAGTCATTGTTCCCa                                                                                                         >  3:418273/1‑141 (MQ=255)

                                      tGTTAGTGCGCGCGCAATTTCTAACGACCAATTCATCGAAATAGTATAAGTCACTGTATTGGCATTTTTTAAAAAGATTAAAAACATAGGTAGTGCACCGGATAAATATGAGAATAATAAGATGTTAGTCATTGTTCCCat                                                                                                        >  1:791231/1‑141 (MQ=255)

                                       gTTAGTGCGCGCGCAATTTCTAACGACCAATTCATCGAAATAGTATAAGTCACTGTATTGGCATTTTTTAAAAAGATTAAAAACATAGGTAGTGCACCGGATAAATATGAGAATAATAAGATGTTAGTCATTGTTCCCata                                                                                                       >  4:427407/1‑141 (MQ=255)

                                        ttAGTGCGCGCGCAATTTCTAACGACCAATTCATCGAAATAGTATAAGTCACAGTATTTGCATTTTTTAAAAAGATTAAAAACATAGGTAGTGCACCGGACAAATATGAGAATAATAAGATGTTAGTCATTGTTCCCataa                                                                                                      <  4:353404/141‑1 (MQ=255)

                                         tAGTGCGCGCGCAATTTCTAACGACCAATTCATCGAAATAGTATAAGTCACTGTATTGGCATTTTTTAAAAAGATTAAAAACATAGGTAGTGCACCGGATAAATATGAGAATAATAAGATGTTAGTCATTGTTCCCataat                                                                                                     <  2:277552/141‑1 (MQ=255)

                                           gTGCGCGCGCAATTTCTAACGACCAATTCATCGAAATAGTATAAGTCACTGTATTGGCATTTTTTAAAAAGATTAAAAACATAGGTAGTGCACCGGATAAATATGAGAATAATAAGATGTTAGTCATTGTTCCCATAatat                                                                                                   >  1:292955/1‑141 (MQ=255)

                                              cgcgcgcAATTTCTAACGACCAATTCATCGAAATAGTATAAGTCACAGTATTTGCATTTTTTAAAAAGATTAAAAACATAGGTAGTGCACCGGACAAATATGAGAATAATAAGATGTTAGTCATTGTTCCCATAATATCtt                                                                                                <  2:515051/141‑1 (MQ=255)

                                                cgcgcAATTTCTAAACACCAATTCATCGAAATAGTATAAGTCACTGTATTGGCATTTTTTAAAAAGATTAAAAACATAGGTAGTGCACCGGATAAATATGAGAATAATAAGATGTTAGTCATTGTTCCCATAATATCTTgg                                                                                              <  3:251031/141‑1 (MQ=255)

                                                 gcgcAATTTCTAACGACCAATTCATCGAAATAGTATAAGTCACTGTATTGGCATTTTTTAAAAAGATTAAAAACATAGGTAGTGCACCGTATAAATATGAGAATAATAAGATGTTAGTCATTGTTCCCATAATATCGTGGc                                                                                             <  2:331395/141‑1 (MQ=255)

                                                  cgcAATTTCTAACGACCAATTCATCGAAATAGTATAAGTCACTGTATTGGCATTTTTTAAAAAGATTAAAAACATAGGTAGTGCACCGGATAAATATGAGAATAATAAGATGTTAGTCATTGTTCCCATAATATCTTGGcc                                                                                            >  2:748049/1‑141 (MQ=255)

                                                   gcAATTTCTAACGACCAATTCATCGAAATAGTATAAGTCACTGTATTGGCATTTTTTAAAAAGATTAAAAACCTAGGTAGTGCACCGGATAAATATGAGAATAATAAGATGTTAGTCATTGTTCCCATAATATCTTGGCCg                                                                                           <  2:371901/141‑1 (MQ=255)

                                                     aaTTTCTAACGACCAATTCATCGAAATAGTATAAGTCACTGTATTGGCATTTTTTAAAAAGATTAAAAACATAGGTAGTGCACCGGATAAATATGAGAATAATAAGATGTTAGTCATTGTTCCCATAATATCTTGGCCGAt                                                                                         <  2:354041/141‑1 (MQ=255)

                                                      aTTTCTAACGACCAATTCATCGAAATAGTATAAGTCACTGTATTGGCATTTTTTAAAAAGATTAAAAACATAGGTAGTGCACCGGATAACTATGAGAATAATAAGATGTTAGTCATTGTTCCCATAATATCTTGGCCGATg                                                                                        <  3:201285/141‑1 (MQ=255)

                                                       gttCTAACGACCAATTCATCGAAATAGTATAAGTCACTGTATTGGCATTTTTTAAAAAGATTAAAAACATAGGTAGTGCACCGGATAAATATGAGAATAATAAGATGTTAGTCATTGTTCCCATAATATCTTGGCCGATGt                                                                                       >  3:348067/2‑141 (MQ=255)

                                                         tCTAACGACCAATTCATTGAATTAGTTTAAGTCACTGTATTGGCATTTTTTAAAAAGATTAAAAACATAGGTAGTGCACCGGATAAATATGAGAATAATAAGATGTTAGTCATTGTTCCCATAATATCTTGGCCGATGttt                                                                                     >  2:518232/1‑141 (MQ=255)

                                                              cGACCAATTCATCGAAATAGTATAAGTCACTGTATTGGCATTTTTTAAAAAGATTAAAAACATAGGTAGTGCACCGGATAAATATGAGAATAATAAGATGTTAGTCATTGTTCCCATAATATCTTGGCCGATGTTTCGccc                                                                                <  4:252734/141‑1 (MQ=255)

                                                                aCCAATTCATCGAAATAGAATAAGTCACTGTATTGGCATTTTTAAAAAAAAAAAAAAACATAGGTAGTGCACCGGATAAATATGAGAATAATAAGATGTTAGTCATTGTTCCCATAATATCTTGGCCGATGTTTCGCCCAg                                                                              <  2:979772/141‑1 (MQ=255)

                                                                  cAATTCATCGAAATAGTATAAGTCACTGTATTGGCATTTTTTAAAAAGATTAAAAACATAGGTAGTGCACCGGATAAATATGAGAATAATAAGATGTTAGTCATTGTTCCCATAATATCTTGGCCGATGTTTCGCCCAGCa                                                                            <  2:917209/141‑1 (MQ=255)

                                                                  cAATTCATCGAAATAGTATAAGTCACTGTATTGGCATTTTTTAAAAAGATTAAAAACATAGGTAGTGCACCGGATAAATATGAGAATAATAAGATGTTAGTCATTGTTCCCATAATATCTTGGCCGATGTTTCGCCCAGCa                                                                            <  2:124668/141‑1 (MQ=255)

                                                                  cAATTCATCGAAATAGTATAAGTCACTGTATTGGCATTTTTTAAAAAGATTAAAAACATAGGTAGTGCACCGGATAAATATGAGAATAATAAGATGTTAGTCATTGTTCCCATAATATCTTGGCCGATGTTTCGCCCAGCa                                                                            <  3:126747/141‑1 (MQ=255)

                                                                   aaTTCATCGAAATAGTATAAGTCACTGTATTGGCATTTTTTAAAAAGATTAAAAACATAGGTAGTGCGCCGGATAAATATGAGAATAATAAGATGTTAGTCATTGTTCCCATAATATCTTGGCCGATGTTTCGCCCAGCaa                                                                           >  4:1943/1‑141 (MQ=255)

                                                                    aTTCATCGAAATAGTATAAGTCACTGTATTGGCATTTTTTAAAAAGATTAAAAACATAGGTAGTGCACCGGATAAATATGAGGATAATAAGATGTTAGTCATTGTTCCCATAATATCTTGGCCGATGTTTCGCCCAGCAAg                                                                          >  2:737730/1‑141 (MQ=255)

                                                                    aTTCATCGAAATAGTATAAGTCACTGTATTGGCATTTTTTAAAAAGATTAAAAACATAGGTAGTGCACCGGATAAATATGAGAATAATAAGATGTTAGTCATTGTTCCCATAATATCTTGGCCGATGTTTCGCCCAGCAAg                                                                          >  1:626333/1‑141 (MQ=255)

                                                                     ttCATCGAAATAGTATAAGTCACTGTATTGGCATTTTTTAAAAAGATTAAAAACATAGGTAGTGCACCGGATAAATATGAGAATAATAAGATGTTAGTCATTGTTCCCATAATATCTTGGCCGATGTTTCGCCCAGCAAgc                                                                         >  3:340268/1‑141 (MQ=255)

                                                                       cATCGAAATAGTATAAGTCACTGTATTGGCATTTTTTAAAAAGATTAAAAACATAGGTAGTGCACCGGATAAATATGAGAATAATAAGATGTTAGTCATTGTTCCCATAATATCTTGGCCGATGTTTCGCCCAGCAAgcgc                                                                       <  2:745902/141‑1 (MQ=255)

                                                                        aTCGAAATAGTATAAGTCACTGTATTGGCATTTTTTAAAAAGATTAAAAACATAGGTAGTGCACCGGATAAATATGAGAATAATAAGATGTTAGTCATTGTTCCCATAATATCTTGGCCGATGTTTCGCCCAGCAAGCGcc                                                                      <  1:84319/141‑1 (MQ=255)

                                                                        aTCGAAATAGTATAAGTCACTGTATTGGCATTTTTTAAAAAGATTAAAAACATAGGTAGTGCACCGGATAAATATGAGAATAATAAGATGTTAGTCATTGTTCCCATAATATCTTGGCCGATGTTTCGCCCAGCAAGCGcc                                                                      <  3:341032/141‑1 (MQ=255)

                                                                         tCGAAATAGTATACGTCACTGTATTGGCATTTTTTAAAAAGATTAAAAACATAGGTAGTGCACCGGATAAATATGAGAATAATAAGCGGTTAGTCATTGTTCCCATAATATCTTGGCCGATGTTTCGCCCAGCAAGCGccc                                                                     <  3:189884/141‑1 (MQ=255)

                                                                          cGAAATAGTATAAGTCACTGTATTGGCATTTTTTTTTTTTTTTAAAAAAATAGATAGTGCACCGGATAAATATGAGAATAATAAGATGTTAGTCATTGTTCCCATAATATCTTGGCCTATGTTATTACCAGCAAACGCCCa                                                                    >  2:238812/1‑141 (MQ=255)

                                                                          cGAAATAGTATAAGTCACTGTATTGGCATTTTTTAAAAAGATTAAAAACATAGGTAGTGCACCGGATAAATATGAGTATAATAAGATGTTAGTCATTGTTCCCATAATATCTTGGCCGATGTTTCGCCCAGCAAGCGCCCa                                                                    >  3:270172/1‑141 (MQ=255)

                                                                           gAAATAGTATAAGTCACTGTATTGGCATTTTTTAAAAAGATTAAAAACATAGGTAGTGCACCGGATAAATATGAGAATAATAAGATGTTAGTCATTGTTCCCATAATATCTTGGCCGATGTTTCGCCCAGCAAGCGCCCAt                                                                   <  1:208065/141‑1 (MQ=255)

                                                                           gAAATAGTATAAGTCACTGTATTGGCATTTTTTAAAAAGATTAAAAACATAGGTAGTGCACCGGATAAATATGAGAATAATAAGATGTTAGTCATTGTTCCCATAATATCTTGGCCGATGTTTCGCCCAGCAAGCGCCCAt                                                                   <  1:208096/141‑1 (MQ=255)

                                                                            aaaTAGTATAAGTCACTGTATTGGCATTTTTTAAAAAGATTAAAAACATAGGTAGTGCACCGGATAAATATGAGAATAATAAGATGTTAGTCATTGTTCCCATAATATCTTGGCCGATGTTTCGCCCAGCAAGCGCCCAtc                                                                  <  4:58699/141‑1 (MQ=255)

                                                                             aaTAGTATAAGTCACTGTATTGGCATTTTTTAAAAAGATTAAAAACATAGGTAGTGCACCGGATAAATATGAGAATAATAAGATGTTAGTCATTGTTCCCATAATATCTTGGCCGATGTTTCGCCCAGCAAGCGACCAtct                                                                 >  3:62178/1‑141 (MQ=255)

                                                                                aGTATAAGTCACAGTATTTGCATTTTTTAAAAAGATTAAAAACATAGGTAGTGCACCGGACACATATGAGAATAATAAGATGTTAGTCATTGTTCCCATAATATCTTGGCCGATGTTTCGCCCAGCAAGCGCCCATctcct                                                              <  2:575154/141‑1 (MQ=255)

                                                                                     aaGTCACTGTATTGGCATTTTTTAAAAAGATTAAAAACATAGGTAGTGCACCGGATAAATATGAGAATAATAAGATGTTAGTCATTGTTCCCATAATATCTTGGCCGATGTTTCGCCCAGCAAGCGCTCATCTCCTCATTg                                                         >  4:242703/1‑141 (MQ=255)

                                                                                     aaGTCACTGTATTGGCATTTTTTAAAAAGATTAAAAACATAGGTAGTGCACCGGATAAATATGAGAATAATAAGATGTTAGTCATTGTTCCCATAATATCTTGGCCGATGTTTCGCCCAGCAAGCGCCCATCTCCTCATTg                                                         <  1:357846/141‑1 (MQ=255)

                                                                                                   gCATTTTTTAAAAAGATTAAAAACATAGGTAGTGCACCGGATAAATATGAGAATAATAAGATGTTAGTCATTGTTCCCATAATATCTTGGCCGATGTTTCGCCCAGCAAGCGCCCATCTCCTCATTGAAATGTGTGGCGTa                                           >  1:700203/1‑141 (MQ=255)

                                                                                                   gCATTTTTTAAAAAGATTAAAAACATAGGTAGTGCACCGGATAAATATGAGAATAATAAGATGTTAGTCATTGTTCCCATAATATCTTGGCCGATGTTTCGCCCAGCAAGCGCCCATCTCCTCATTGAAATGTGTGGCGTa                                           >  2:107438/1‑141 (MQ=255)

                                                                                                     aTTTTTTAAAAAGATTAAAAACATAGGTAGTGCACCGGATAAATATGAGAATAATAAGATGTTAGTCATTGTTCCCATAATATCTTGGCCGATGTTTCGCCCAGCAAGCGCCCATCTCCTCATTGAAATGTGTGGCGTACg                                         <  1:165497/141‑1 (MQ=255)

                                                                                                      ttttttAAAAAGATTAAAAACATAGGTAGTGCACCGGATAAATATGAGAATAATAAGATGTTAGTCATTGTTCCCATAATATCTTGGCCGATGTTTCGCCCAGCAAGCGCCCATCTCCTCATTGAAATGTGTGGCGTACGc                                        >  2:459162/1‑141 (MQ=255)

                                                                                                      ttttttAAAAAGATTAAAAACATAGGTAGTGCACCGGATAAATATGAGAATAATAAGATGTTAGTCATTGTTCCCATAATATCTTGGCCGATGTTTCGCCCAGCAAGCGCCCATCTCCTCATTGAAATGTGTGGCGTACGc                                        <  2:136840/141‑1 (MQ=255)

                                                                                                        ttttAAAAAGATTAAAAACATAGGTAGTGCACCGGATAAATATGAGAATAATAAGATGTTAGTCATTGTTCCCATAATATCTTGGCCGATGTTTCGCCCAGCAAGCGCCCATCTCCTCATTGAAATGTGTGGCGTACGCTg                                      >  1:253750/1‑141 (MQ=255)

                                                                                                        ttttAAAAAGATTAAAAACATAGGTAGTGCACCGGATAAATATGAGAAGAATAAGATGTTAGTCATTGTTCCCATAATATCTTGGCCGATGTTTCGCCCAGCAAGCGCCCATCTCCTCATTGAAATGTGTGGCGTACGCTg                                      <  1:972437/141‑1 (MQ=255)

                                                                                                         tttAAAAAGATTAAAAACATAGGTAGTGCACCGGATAAATATGAGAATAATAAGATGTTAGTCATTGTTCCCATAATATCTTGGCCGATGTTTCGCCCAGCAAGCGCCCATCTCCTCATTGAAATGTGTGGCGTACGCTGt                                     <  1:518232/141‑1 (MQ=255)

                                                                                                          ttAAAAAGATTAAAAACATAGGTAGTGCACCGGATAAATATGAGAATAATAAGATGTTAGTCATTGTTCCCATAATATCTTGGCCGATGTTTCGCCCAGCAAGCGCCCATCTCCTCATTGAAATGTGTGGCGTACGCTGTa                                    >  1:617645/1‑141 (MQ=255)

                                                                                                          ttAAAAAGAGTAAAAACATAGGTAGTGCACCGGATAAATATGTGAATAATAAGATGTTAGTCATTGTTCCCATAATATCTTGGCCGATGTTTCGCCCAGCAAGCGCCCATCTCCTCAGTGAAATGTGTGGCGTACGCTGTa                                    <  2:1039413/141‑1 (MQ=255)

                                                                                                           tAAAAAGATTAAAAACATAGGTAGTGCACCGGATAAATATGAGAATAATAAGATGTTAGTCATTGTTCCCATAATATCTTGGCCGATGTTTCGCCCAGCAAGCGCCCATCTCCTCATTGAAATGTGTGGCGTACGCTGTaa                                   >  2:51729/1‑141 (MQ=255)

                                                                                                            aaaaaGATTAAAAACATAGGTAGTGCACCGGATAAATATGAGAATAATAAGATGTTAGTCATTGTTCCCATAATATCTTGGCCGATGTTTCGCCCAGCAAGCGCCCATCTCCTCATTGAAATGTGTGGCGTACGCTGTaaa                                  <  1:83446/141‑1 (MQ=255)

                                                                                                            aaaaaGATTAAAAACATAGGTAGTGCACCGGATAAATATGAGAATAATAAGATGTTAGTCATTGTTCCCATAATATCTTGGCCGATGTTTCGCCCAGCAAGCGCCCATCTCCTCATTGAAATGTGTGGCGTACGCTGTaaa                                  >  2:707922/1‑141 (MQ=255)

                                                                                                            aaaaaGATTAAAAACATAGGTAGTGCACCGGATAAATATGAGAATAATAAGATGTTAGTCATTGTTCCCATAATATCTTGGCCGATGTTTCGCCCAGCAAGCGCCCATCTCCTCATTGAAATGTGTGGCGTACGCTGTaaa                                  >  2:190629/1‑141 (MQ=255)

                                                                                                               aaGATTAAAAACATAGGTAGTGCACCGGATAAATATGAGAATAATAAGATGTTAGTCATTGTTCCCATAATATCTTGGCCGATGTTTCGCCCAGCAAGCGCCCATCTCCTCATTGAAATGTGTGGCGTACGCTGTAAAAtt                               >  1:336290/1‑141 (MQ=255)

                                                                                                                 gATTAAAAACATAGGTAGTGCACCGGATAAATATGAGAATAATAAGATGTTAGTCATTGTTCCCATAATATCTTGGCCGATGTTTCGCCCAGCAAGCGCCCATCTCCTCATTGAAATGTGTGGCGTACGCTGTAAAATTTc                             >  4:385319/1‑141 (MQ=255)

                                                                                                                     aaaaaCATAGGTAGTGCACCGGATAAATATGAGAATAATAAGATGTTAGTCATTGTTCCCATAATATCTTGGCCGATGTTTCGCCCAGCAAGCGCCCATCTCCTCATTGAAATGTGTGGCGTACGCTGTAAAATTTCATGc                         <  1:1075855/141‑1 (MQ=255)

                                                                                                                     aaaaaCATAGGTAGTGCACCGGATAAATATGAGAATAATAAGATGTTAGTCATTGTTCCCATAATATCTTGGCCGATGTTTCGCCCAGCAAGCGCCCATCTCCTCATTGAAATGTGTGGCGTACGCTGTAAAATTTCATGc                         >  1:984432/1‑141 (MQ=255)

                                                                                                                      aaaaCATAGGTAGTGCACCGGATAAATATGAGAATAATAAGATGTTAGTCATTGTTCCCATAATATCTTGGCCGATGTTTCGCCCAGCAAGCGCCCATCTCCTCATTGAAATGTGTGGCGTACGCTGTAAAATTTCATGCa                        >  1:1076627/1‑141 (MQ=255)

                                                                                                                      aaaaCATAGGTAGTGCACCGGATAAATATGAGAATAATAAGATGTTAGTCATTGTTCCCATAATATCTTGGCCGATGTTTCGCCCAGCAAGCGCCCATCTCCTCATTGAAATGTGTGGCGTACGCTGTAAAATTTCATGCa                        <  1:619646/141‑1 (MQ=255)

                                                                                                                         aCATAGGTAGTGCACCGGATAAATATGAGAATAATAAGATGTTAGTCATTGTTCCCATAATATCTTGGCCGATGTTTCGCCCAGCAAGCGCCCATCTCCTCATTGAAATGTGTGGCGTACGCTGTAAAATTTCATGCATAc                     <  2:475192/141‑1 (MQ=255)

                                                                                                                         aCATAGGTAGTGCACCGGATAAATATGAGAATAATAAGATGTTAGTCATTGTTCCCATAATATCTTGGCCGATGTTTCGCCCAGCAAGCGCCCATCTCCTCATTGAAATGTGTGGCGTACGCTGTAAAATTTCATGCATAc                     <  4:24262/141‑1 (MQ=255)

                                                                                                                           aTAGGTAGTGCACCGGATAAATATGAGAATAATAAGATGTTAGTCATTGTTCCCATAATATCTTGGCCGATGTTTCGCCCAGCAAGCGCCCATCTCCTCATTGAAATGTGTGGCGTACGCTGTAAAATTTCATGCATACCa                   <  4:405483/141‑1 (MQ=255)

                                                                                                                           aTAGGTAGTGCACCGGATAAATATGAGAATAATAAGATGTTAGTCATTGTTCCCATAATATCTTGGCCGATGTTTCGCCCAGCAAGCGCCCATCTCCTCATTGAAATGTGTGGCGTACGCTGTAAAATTTCATGCATACCa                   <  2:426066/141‑1 (MQ=255)

                                                                                                                              ggTAGTGCACCGGATAAATATGAGAATAATAAGATGTTAGTCATTGTTCCCATAATATCTTGGCCGATGTTTCGCCCAGCAAGCGCCCATCTCCTCATTGAAATGTGTGGCGTACGCTGTAAAATTTCATGCATACCACTa                <  4:218964/141‑1 (MQ=255)

                                                                                                                                tAGTGCACCGGATAAATATGAGAATAATAAGATGTTAGTCATTGTTCCCATAATATCTTGGCCGATGTTTCGCCCAGCAAGCGCCCATCTCCTCATTGAAATGTGTGGCGTACGCTGTAAAATTTCATGCATACCACTAGc              >  1:73013/1‑141 (MQ=255)

                                                                                                                                 aGTGCACCGGATAAATATGAAAATAATAAGATGTTAGTCATTGTTCCCATAATATCTTGGCCGATGTTTCGCCCAGCAAGCGCCCATCTCCTCATTGAAATGTGTGGCGTACGCTGTAAAATTTCATGCATACCACTAGCa             <  1:775113/141‑1 (MQ=255)

                                                                                                                                    gCACCGGATAAATATGAGAATAATAAGATGTTAGTCATTGTTCCCATAATATCTTGGCCGATGTTTCGCCCAGCAAGCGCCCATCTCCTCATTGAAATGTGTGGCGTACGCTGTAAAATTTCATGCATACCACTAGCAAtt          >  2:834564/1‑141 (MQ=255)

                                                                                                                                    gCACCGGATAAATATGAGAATAATAAGATGTTAGTCATTGTTCCCATAATATCTTGGCCGATGTTTCGCCCAGCAAGCGCCCATCTCCTCATTGAAATGTGTGGCGTACGCTGTAAAATTTCATGCATACCACTAGCAAtt          >  2:834547/1‑141 (MQ=255)

                                                                                                                                    gCACCGGACAAATATGAGAATAATAAGATGTTAGTCATTGTTGCCATAATATCTTGGCCGATGTTTCTCCCAGCAAGCGCCCATCTCCTCATTGAAATGTGTGGCGTACGCTGTAAAATTTCATGCATACCACTAGCAAtt          >  1:933968/1‑141 (MQ=255)

                                                                                                                                     cACCGGATAAATATGAGAATAATAAGATGTTAGTCATTGTTCCCATAATATCTTGGCCGATGTTTCGCCCAGCAAGCGCCCATCTCCTCATTGAAATGTGTGGCGTACGCTGTAAAATTTCATGCATACCACTAGCAATTg         <  2:868641/141‑1 (MQ=255)

                                                                                                                                     cACCGGATAAATATGAGAATAATAAGATGTTAGTCATTGTTCCCATAATATCTTGGCCGATGTTTCGCCCAGCAAGCGCCCATCTCCTCATTGAAATGTGTGGCGTACGCTGTAAAATTTCATGCATACCACTAGCAATTg         <  4:242590/141‑1 (MQ=255)

                                                                                                                                       ccGGATAAATATGAGAATAATAAGATGTTAGTCATTGTTCCCATAATATCTTGGCCGATGTTTCGCCCAGCAAGCGCCCATCTCCTCATTGAAATGTGTGGCGTACGCTGTAAAATTTCATGCATACCACTAGCAATTGTa       >  3:439327/1‑141 (MQ=255)

                                                                                                                                        cGGATAAATATGAGAATAATAAGATGTTAGTCATTGTTCCCATAATATCTTGGCCGATGTTTCGCCCAGCAAGCGCCCATCTCCTCATTGAAATGTGTGGCGTACGCTGTAAAATTTCATGCATACCACTAGCAATTGTaa      >  3:83180/1‑141 (MQ=255)

ATTGTGATAGGAATTGTAAGTACGATACCTATACCACCTGTTAGTGCGCGCGCAATTTCTAACGACCAATTCATCGAAATAGTATAAGTCACTGTATTGGCATTTTTTAAAAAGATTAAAAACATAGGTAGTGCACCGGATAAATATGAGAATAATAAGATGTTAGTCATTGTTCCCATAATATCTTGGCCGATGTTTCGCCCAGCAAGCGCCCATCTCCTCATTGAAATGTGTGGCGTACGCTGTAAAATTTCATGCATACCACTAGCAATTGTAATTGC  >  USA300TCH1516_ALE/495045‑495325

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`