| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 543,821 | A→T | 5.9% | L370F (TTA→TTT) | mazG → | Nucleoside triphosphate pyrophosphohydrolase/pyrophosphatase MazG |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 543,821 | 0 | A | T | 5.9% | 478.7 / 5.7 | 153 | L370F (TTA→TTT) | mazG | Nucleoside triphosphate pyrophosphohydrolase/pyrophosphatase MazG |
| Reads supporting (aligned to +/- strand): ref base A (67/75); new base T (9/2); total (76/77) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.14e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 157 total aligned reads displayed.
ATTCGTAGACACCCACACATATTTGGTGATGCCAATGCTGAAACTATCGATGACTTAAAAGAAATTTGGTCTAAGGCGAAAGATGCTGAAGGTAAACAGCCAAGAGTTATATTTGAAAAAGTATTTGCAGAGCATTTTTTAAATTTATATGAGAAGACGAAGGATAAGTCATTTGATGAGGCCGCGTTAAAGCAGTGGCTAGAAAAAGGGGAGAGTAATACATGAGATTAGATAAATATTTAAAAGTATCACGGTTAATAAAGCGACGTACGCTAGCAAAA > USA300TCH1516_ALE/543681‑543961 | tCGTAGACACCCACACATATTTGGTGATGCCAATGCTGAAACTATCGATGACTTAAAAGAAATTTGGTCTAAGGCGAAAGATGCTGAAGGTAAACAGCCAAGAGTTATATTTGAAAAAGTATTTGCAGAGCATTTTTTaaa < 1:1066013/141‑1 (MQ=255) tCGTAGACACCCACACATATTTGGTGATGCCAATGCTGAAACTATCGATGACTTAAAAGAAATTTGGTCTAAGGCGAAAGATGCTGAAGGTAAACAGCCAAGAGTTATATTTGAAAAAGTATTTGCAGAGCATTTTTTaaa < 1:359855/141‑1 (MQ=255) cGTAGACACCCACACATATTTGGTGATGCCAATGCTGAAACTATCGATGACTTAAAAGAAATTTGGTCTAAGGCGAAAGATGCTGAAGGTAAACAGCCAAGAGTTATATTTGAAAAAGTATTTGCAGAGCATTTTTTAAAt < 2:507246/141‑1 (MQ=255) gTAGACACCCACACATATTTGGTGATGCCAATGCTGAAACTATCGATGACTTAAAAGAAATTTGGTCTAAGGCGAAAGATGCTGAAGGTAAACAGCCAAGAGTTATATTTGAAAAAGTATTTGCAGAGCATTTTTTAAAtt > 1:62090/1‑141 (MQ=255) gTAGACACCCACACATATTTGGTGATGCCAATGCTGAAACTATCGATGACTTAAAAGAAATTTGGTCTAAGGCGAAAGATGCTGAAGGTAAACAGCCAAGAGTTATATTTGAAAAAGTATTTGCAGAGCATTTTTTAAAtt < 2:639065/141‑1 (MQ=255) acacCCACACATATTTGGTGATGCCAATGCTGAAACTATCGATGACTTAAAAGAAATTTGGTCTAAGTCGAAAGATGCTGAAGGTAAACAGCCAAGAGTTATATTTGAAAAAGTATTTGCAGAGCATTTTTTAAATTtata > 3:44216/1‑141 (MQ=255) acacCCACACATATTTGGTGATGCCAATGCTGAAACTATCGATGACTTAAAAGAAATTTGGTCTAAGGCGAAAGATGCTGAAGGTAAACAGCCAAGAGTTATATTTGAAAAAGTATTTGCAGAGCATTTTTTAAATTtata > 2:706088/1‑141 (MQ=255) acacCCACACATATTTGGTGATGCCAATGCTGAAACTATCGATGACTTAAAAGAAATTTGGTCTAAGGCGAAAGATGCTGAAGGTAAACAGCCAAGAGTTAAATTTGAAAAAGTATTTGCAGAGCATTTTTTTAATTtata < 1:642521/141‑1 (MQ=255) cccACACATATTTGGTGATGCCAATGCTGAAACTATCGATGACTTAAAAGAAATTTGGTCTAAGGCGAAAGATGCTGAAGGTAAACAGCCAAGAGTTATATTTGAAAAAGTATTTGCAGAGCATTTTTTAAATTTATATga < 2:871133/141‑1 (MQ=255) acacaTATTTGGTGATGCCAATGCTGAAACTATCGATGACTTAAAAGAAATTTGGTCTAAGGCGAAAGATGCTGAAGGTAAACAGCCAAGAGTTATATTTGAAAAAGTATTTGCAGAGCATTTTTTAAATTTATATGagaa < 3:171370/141‑1 (MQ=255) tatTTGGTGATGCCAATGCTGAAACTATCGATGACTTAAAAGAAATTTGGTCTAAGGCGAAAGATGCTGAAGGTAAACAGCCAAGAGTTATATTTGAAAAAGTATTTGCAGAGCATTTTTTAAATTTATATGAGAAGACGa < 1:945948/141‑1 (MQ=255) tatTTGGTGATGCCAATGCTGAAACTATCGATGACTTAAAAGAAATTTGGTCTAAGGCGAAAGATGCTGAAGGTAAACAGCCAAGAGTTATATTTGAAAAAGTATTTGCAGAGCATTTTTTAAATTTATATGAGAAGACGa < 1:74165/141‑1 (MQ=255) tatTTGGTGATGCCAATGCTGAAACTATCGATGACTTAAAAGAAATTTGGTCTAAGGCGAAAGATGCTGAAGGTAAACAGCCAAGAGTTATATTTGAAAAAGTATTTGCAGAGCATTTTATATATTTATATGAGacgacgc > 2:749418/1‑140 (MQ=255) tttGGTGATGCCAATGCTGAAACTATCGATGACTTAAAAGAAATTTGGTCTAAGGCGAAAGATGCTGAAGGTAAACAGCCAAGAGTTATATTTGAAAAAGTATTTGCAGAGCATTTTTTAAATTTATATGAGAAGACGAAg < 4:439014/141‑1 (MQ=255) ttGGTGATGCCAATGCTGAAACTATCGATGACTTAAAAGAAATTTGGTCTAAGGCGAAAGATGCTGAAGGTAAACAGCCAAGAGTTATATTTGAAAAAGTATTTGCAGAGCATTTTTTATTTTTATATGAGAAGAAGAAgg > 2:974572/1‑141 (MQ=255) ttGGTGATGCCAATGCTGAAACTATCGATGACTTAAAAGAAATTTGGTCTAAGGCGAAAGATGCTGAAGGTAAACAGCCAAGAGTTATATTTGAAAAAGTATTTGCAGAGCATTTTTTAAATTTATATGAGAAGACGAAgg > 2:767809/1‑141 (MQ=255) tGGGGATGCCAATGCTGAAACTATCGATGACTTAAAAGAAATTTGGTCTAAGGCGAAAGATGCTGAAGGTAAACAGCCAAGAGTTATATTTGAAAAAGTATTTGCAGAGCATTTTTTAAATTTATATGAGAAGACGAAGGa > 2:189411/1‑141 (MQ=255) ggTGATGCCAATGCTGAAACTATCGATGACTTAAAAGAAATTTGGTCTAAGGCGAAAGATGCTGAAGGTAAACAGCCAAGAGTTCTATTTGAAAAAGTATTTGCAGAGCATTTTTTAAATTTATATGAGAAGACGAAGGAt > 1:117287/1‑141 (MQ=255) ggTGATGCCAATGCTGAAACTATCGATGACTTAAAAGAAATTTGGTCTAAGGCGAAAGATGCTGAAGGTAAACAGCCAAGAGTTATATTTGAAAAAGTATTTGCAGAGCATTTTTTAAATTTATATGAGAAGACGAAGGAt < 3:91077/141‑1 (MQ=255) ggTGATGCCAATGCTGAAACTATCGATGACTTAAAAGAAATTTGGTCTAAGGCGAAAGATGCTGAAGGTAAACAGCCAAGAGTTATATTTGAAAAAGTATTTGCAGAGCATTTTTTAAATTTATATGAGAAGACGAAGGAt < 3:329930/141‑1 (MQ=255) gATGCCAATGCTGAAACTATCGATGACTTAAAAGAAATTTGGTCTAAGGCGAAAGATGCTGAAGGTAAACAGCCAAGAGTTATATTTGAAAAAGTATTTGCAGAGCATTTTTTAAATTTATATGGGAAGACGAAGGATAAg > 2:483749/1‑141 (MQ=255) gATGCCAATGCTGAAACTATCGATGACTTAAAAGAAATTTGGTCTAAGGCGAAAGATGCTGAAGGTAAACAGCCAAGAGTTATATTTGAAAAAGTATTTGCAGAGCATTTTTTAAATTTATATGAGAAGACGAAGGATAAg > 2:588999/1‑141 (MQ=255) gATGCCAATGCTGAAACTATCGATGACTTAAAAGAAATTTGGTCTAAGGCGAAAGATGCTGAAGGTAAACAGCCAAGAGTTATATTTGAAAAAGTATTTGCAGAGCATTTTTTAAATTTATATGAGAAGACGAAGGATAAg < 1:829496/141‑1 (MQ=255) tGCCAATGCTGAAACTATCGATGACTTAAAAGAAATTTGGTCTAAGGCGAAAGATGCTGAAGGTAAACAGCCAAGAGTTATATTTGAAAAAGTATTTGCAGAGCATTTTTTAAATTTATATGAGAAGACGAAGGATAAGTc < 1:166520/141‑1 (MQ=255) ccAATGCTGAAACTATCGATGACTTAAAAGAAATTTGGTCTAAGGCGAAAGATGCTGAAGGTAAACAGCCAAGAGTTATATTTGAAAAAGTATTTGCAGAGCATTTTTTAAATTTATATGAGAAGACGAAGGATAAGTCAt < 2:78776/141‑1 (MQ=255) ccAATGCTGAAACTATCGATGACTTAAAAGAAATTTGGTCTAAGGCGAAAGATGCTGAAGGTAAACAGCCAAGAGTTATATTTGAAAAAGTATTTGCAGAGCATTTTTTAAATTTATATGAGAAGACGAAGGATAAGTCAt > 3:257945/1‑141 (MQ=255) tGAAACTATCGATGACTTAAAAGAAATTTGGTCTAAGGCGAAAGATGCTGAAGGTAAACAGCCAAGAGTTATATTTGAAAAAGTATTTGCAGAGCATTTTTTAAATTTATATGAGAAGACGAAGGATAAGTCATTtgatga < 1:960271/141‑1 (MQ=255) aaCTATCGATGACTTAAAAGAAATTTGGTCTAAGGCGAAAGATGCTGAAGGTAAACAGCCAAGAGTTATATTTGAAAAAGTATTTGCAGAGCATTTTTTAAATTTATATGAGAAGACGAAGGATAAGTCATTTGATGAGGc < 1:428327/141‑1 (MQ=255) aaCTATCGATGACTTAAAAGAAATTTGGTCTAAGGCGAAAGATGCTGAAGGTAAACAGCCAAGAGTTATATTTGAAAAAGTATTTGCAGAGCATTTTTTAAATTTATATGAGAAGACGAAGGATAAGTCATTTGATGAGGc > 4:362881/1‑141 (MQ=255) aCTATAGATGACTTAAAAGAAATTTGGTCTAAGGCGAAAGATGCTGAAGGTAAACAGCCAAGAGCTATATTTGAACAAGTATTTGCAGAGCATTTTTTAAATTTATATGGGACGACGAAGGATAAGTCAGTTGTTGAGGcc > 4:16009/1‑141 (MQ=255) tCGATGACTTAAAAGACATTTGGTCTAAGGCGAAAGATGCTGACGGTAAACAGCCAAGAGTTATATTTGAAAAAGTATTTGCAGAGCATTTTTTAAATTTATATGAGAAGACGAAGGATAAGTCATTTGATGAGGCCGCGt > 2:35485/1‑141 (MQ=255) tCGATGACTTAAAAGAAATTTGGTCTAAGGCGAAAGATGCTGAAGGTAAACAGCCAAGAGTTATATTTGAAAAAGTATTTGCAGAGCATTTTTTAAATTTATATGAGAAGACGAAGGATAAGTCATTTGATGAGGCCGCGt < 1:42522/141‑1 (MQ=255) cGATGACTTAAAAGAAATTTGGTCTAAGGCGAAAGATGCTGAAGGTAAACAGCCAAGAGTTATATTTGAAAAAGTATTTGCAGAGCATTTTTTAAATTTATATGAGAAGACGAAGGATAAGTCATTTGATGAGGCCGCGtt < 2:359064/141‑1 (MQ=255) gATGACTTAAAAGAAATTTGGTCTAAGGCGAAAGATGCTGAAGGTAAACAGCCAAGAGTTATATTTGAAAAAGTATTTGCAGAGCATTTTTTAAATTTATATGAGAAGACGAAGGATAAGTCATTTGATGAGGCCGCGTTa > 2:1079129/1‑141 (MQ=255) aaaGAAATTTGGTCTAAGGCGAAAGATGCTGAAGGTAAACAGCCAAGAGTTATATTTGAAAAAGTATTTGCAGAGCATTTTTTAGATGTATATGAGAAGACGAAGGATAAGTCATTTGATGAGGCCGCGTTAAAGCAGTgg < 3:120415/141‑1 (MQ=255) aaaGAAATTTGGTCTAAGGCGAAAGATGCTGAAGGTAAACAGCCAAGAGTTATATTTGAAAAAGTATTTGCAGAGCATTTTTTAAATTTATATGAGAAGACGAAGGATAAGTCATTTGATGAGGCCGCGTTAAAGCAGTgg > 2:943986/1‑141 (MQ=255) aaaGAAATTTGGTCTAAGGCGAAAGATGCTGAAGGTAAACAGCCAAGAGTTATATTTGAAAAAGTATTTGCAGAGCATTTTTTAAATTTATATGAGAAGACGAAGGATAAGTCATTTGATGAGGCCGCGTTAAAGCAGTgg < 2:959122/141‑1 (MQ=255) gAAATTTGGTCTAAGGCGAAAGATGCTGAAGGTAAACAGCCAAGAGTTATATTTGAAAAAGTATTTGCAGAGCATTTTTTAAATTTATATGAGAAGACGAAGGATAAGTCATTGGCTGAGGCCGCGTTAAAGCAGTGGCTa < 4:390016/141‑1 (MQ=255) aaTTTGGTCTAAGGCGAAAGATGCTGAAGGTAAACAGCCAAGAGTTATATTTGAAAAAGTATTTGCAGAGCATTTTTTAAATTTATATGAGAAGACGAAGGATAAGTCATTTGATGAGGCCGCGTTAAAGCAGTGGCTAGa > 1:608613/1‑141 (MQ=255) aaTTTGGTCTAAGGCGAAAGATGCTGAAGGTAAACAGCCAAGAGTTATATTTGAAAAAGTATTTGCAGAGCATTTTTTAAATTTATATGAGAAGACGAAGGATAAGTCATTTGATGAGGCCGCGTTAAAGCAGTGGCTAGa < 1:868191/141‑1 (MQ=255) tttGGTCTAAGGCGAAAGATGCTTAAGGTAAACAGCCAAGAGTTATATTTGAAAAAGTATTTGCAGAGCATTTTTTTTATTTTTTTTAGAAGACGAATGATAAGTCATTTGATGAGGCCGCGTTAAAGCAGTGGTTAGaaa > 2:196629/1‑141 (MQ=255) tGGTCTAAGGCGAAAGATGCTGAAGGTAAACAGCCAAGAGTTATATTTGAAAAAGTATTTGCAGAGCATTTTTTAAATTTATATGAGAAGACGAAGGATAAGTCAt < 2:935166/106‑1 (MQ=255) tGGTCTAAGGCGAAAGATGCTGAAGGTAAACAGCCAAGAGTTATATTTGAAAAAGTATTTGCAGAGCATTTTTTAAATTTATATGAGAAGACGAAGGATAAGTCATTTGATGAGGCCGCGTTAAAGCAGTGGCTAGaaaaa > 2:460262/1‑141 (MQ=255) ggTCTAAGGCGAAAGATGCTGAAGGTAAACAGCCAAGAGTTATATTTGAAAAAGTATTTGCAGAGCATTTTTTTATTTTTTATGAGAAGACGAAGGATAAGTCATTTGATGAGGCCGCGTTAAAGCAGTGGCTAGAAAAAg > 4:278512/1‑141 (MQ=255) ggTCTAAGGCGAAAGATGCTGAAGGTAAACAGCCAAGAGTTATATTTGAAAAAGTATTTGCAGAGCATTTTTTAAATTTATATGAGAAGACGAAGGATAAGTCATTTGATGAGGCCGCGTTAAAGCAGTGGCTAGAAAAAg > 2:335390/1‑141 (MQ=255) gTCTAAGGCGAAAGATGCTGAAGGTAAACAGCCAAGAGTTATATTTGAAAAAGTATTTGCAGAGCATTTTTTTTGTTTAGACGGGATGACGAAGGTTAAGTCATTTGATGAGGCCGCGTTAAAGCAGTGGCTAGAAAAAgg > 1:1028799/1‑141 (MQ=255) gTCTAAGGCGAAAGATGCTGAAGGTAAACAGCCAAGAGTTATATTTGAAAAAGTATTTGCAGAGCATTTTTTAAATTTATATGAGAAGACGAAGGATAAGTCATTTGATGAGGCCGCGTTAAAGCAGTGGCTAGAAAAAgg > 1:889755/1‑141 (MQ=255) aGGCGAAAGATGCTGAAGGTAAACAGCCAAGAGTTATATTTGAAAAAGTATTTGCAGAGCATTTTTTAAATTTATATGAGAAGACGAAGGATAAGTCATTTGATGAGGCCGCGTTAAAGCAGTGGCTAGAAAAAGGGgaga > 3:453034/1‑141 (MQ=255) aGGCGAAAGATGCTGAAGGTAAACAGCCAAGAGTTATATTTGAAAAAGTATTTGCAGAGCATTTTTTAAATTTATATGAGAAGACGAAGGATAAGTCATTTGATGAGGCCGCGTTAAAGCAGTGGCTAGAAAAAGGGgaga > 2:540338/1‑141 (MQ=255) gCGAAAGATGCTGAAGGTAAACAGCCAAGAGTTATATTTGAAAAAGTATTTGCAGAGCATTTTTTAAATTTATATGAGAAGACGAAGGATAAGTCATTTGATGAGGCCGCGTTAAAGCAGTGGCTAGAAAAAGGGGAGAGt < 1:297804/141‑1 (MQ=255) gCGAAAGATGCTGAAGGTAAACAGCCAAGAGTTATATTTGAAAAAGTATTTGCAGAGCATTTTTTAAATTTATATGAGAAGACGAAGGATAAGTCATTTGATGAGGCCGCGTTAAAGAAAAAAAAAAAAAAAGGGGAGAGt < 4:364475/141‑1 (MQ=255) aagaagCTGAAGGTAAACAGCCAAGAGTAATATTTGAAAAAGTATTTGCAGAGCATTTTTTAAATTTATATCAGAAGACAAAGGATAAATCATTTGATGAGGCCGCGTTAAAGAAAAAAAAAAAAAAAGGGGAGAGTAATa < 4:218086/141‑1 (MQ=255) aaGATGCTGAAGGTAAACAGCCAAGAGTTATATTTGAAAAAGTATTTGCAGAGCATTTTTTAAATTTATATGAGAAGACGAAGGATAAGTCATTTGATGAGGCCGCGTTAAAGCAGTGGCTAGAAAAAGGGGAGAGTAATa > 2:771545/1‑141 (MQ=255) aGATGCTGAAGGTAAGCAGCCAAGAGTTATATTTGAAAAAGTATTTGCAGAGCATTTTTTTTTTTTATTTGAGAAGACGAAGGGTAAGTCATTTGATGAGGCCGCGTTAAAGCAGTGGCTAGAAAGAGGGGCGCGTAATAc > 2:328398/1‑141 (MQ=255) aGATGCTGAAGGTAAACAGCCAAGAGTTATATTTGAAAAAGTATTTGCAGAGCATTTTTTAAATTTATATGAGAAGACGAAGGATAAGTCATTTGATGAGGCCGCGTTAAAGCAGTGGCTAGAAAAAGGGGAGAGTAATAc > 2:778472/1‑141 (MQ=255) aGATGCTGAAGGTAAACAGCCAAGAGTTATATTTGAAAAAGTATTTGCAGAGCATTTTTTAAATTTATATGAGAAGACGAAGGATAAGTCATTTGATGAGGCCGCGTCAAAGCAGTGGCTAGAAAAAGGGGAGAGTAATAc > 4:345395/1‑141 (MQ=255) gATGCTGAAGGTAAACAGCCAAGAGTTATATTTGAAAAAGTATTTGCAGAGCATTTTTTAAATTTATATGAGAAGACGAAGGATAAGTc > 1:907286/1‑89 (MQ=255) gATGCTGAAGGTAAACAGCCAAGAGTTATATTTGAAAAAGTATTTGCAGAGCATTTTTTAAATTTATATGAGAAGACGAAGGATAAGTc < 2:907286/89‑1 (MQ=255) gATGCTGAAGGTAAACAGCCAAGAGTTATATTTGAAAAAGTATTTGCAGAGCATTTTTTAAATTTATATGAGAAGACGAAGGATAAGTCATTTGATGAGGCCGCGTTAAAGCAGTGGCTAGAAAAAGGGGAGAGTAATACa < 1:27536/141‑1 (MQ=255) gcagAAGGTAAAGAGCCAAGAGTTATATTTGAAAAAGTATTTGCAGAGCAGTTTTTAAATTTATATGAGAAGACGAAGGATAAGTCATTTGATGAGGCCGCGTTAAAAAAAAAAAAAAAAAAAGGGGAGAGTAATACATga < 2:208164/138‑1 (MQ=255) gCTGAAGGTAAACATCCAAGTGTTATATTTGAAAAAGTATTTGCAGAGCAGTTTTTAAATTTATATGAGAAGACGAAGTATAAGTCAATTGATGAGGCTGCGTTAAAACATTGGCTAGAAAAACGGAAGAGTATTACATga > 4:83296/1‑141 (MQ=255) gCTGAAGGTAAACAGCCAAGAGTTATATTTGAAAAAGTATTTGCAGAGCATTTTTTAAATTTATATGAGAAGACGAAGGATAAGTCATTTGATGAGGCCGCGTTAAAGCAGTGGCTAGAAAAAGGGGAGAGTAATACATga < 2:349352/141‑1 (MQ=255) gCTGAAGGTAAACAGCCAAGAGTTATATTTGAAAAAGTATTTGCAGAGCATTTTTTAAATTTATATGAGAAGACGAAGGATAAGTCATTTGATGAGGCCGCGTTAAAGCAGTGGCTAGAAAAAGGGGAGAGTAATACATga > 4:370800/1‑141 (MQ=255) gCTGAAGGTAAACAGCCAAGAGTTATATTTGAAAAAGTATTTGCAGAGCATTTTTTAAATTTATATGAGAAGACGAAGGATAAGTCATTTGATGAGGCCGCGTTAAAGCAGTGGCTAGAAAAAGGGGAGAGTAATACATga > 2:178471/1‑141 (MQ=255) tGAAGGTAAACAGCCAAGAGTTATATTTGAAAAAGTATTTGCAGAGCATTTTTTAAATTTATATGAGAAGACGAAGGATAAGTCATTTGATGAGGCCGCGTTAAAGCAGTGGCTAGAAAAAGGGGAGAGTAATACATgaga > 1:583161/1‑141 (MQ=255) ggTAAACAACCAAGAGTTATAATTGAAAAAGTATTTGAAGAGAATTTTTTAAATTTATATGAGAAGACGAAGGATAAGTCATTTGATGAGGACGCGTTAAAGAAAAAAAAAAAAAAAGGGGAGAGTAATACATGAGATTAg < 4:352993/141‑1 (MQ=255) aacaacAAAAAGTTATATTTAAAAAAGTATTTAAAGAGCATTTTTTAAATTTATATGAAAAGACGAAGGATAAGTCATTTGATGAGGCCGCGTTAAAGAAGAAACAAAAAAAAGGGGAGAGTAATACATGAGATTAGATaa < 2:977393/141‑1 (MQ=255) aCAGCCAAGAGTTATATTTGAAAAAGTATTTGCAGAGCATTTTTTTTTTTTTTTTGAGAAGACGAAGGATAAGTCATTTGATGAGGCCGCGTTAAAGCAGTGGCTAGAAAAAGGGGAGAGTAATACATGAGATTAGATaaa > 4:179395/1‑141 (MQ=255) aCAGCCAAGAGTTATATTTGAAAAAGTATTTGCAGAGCATTTTTTAAATTTATATGAGAAGACGAAGGATAAGTCATTTGATGAGGCCGCGTTAAAGCAGTGGCTAGAAAAAGGGGAGAGTAATACATGAGATTAGATaaa < 3:38065/141‑1 (MQ=255) aCAGCCAAGAGTTATATTTGAAAAAGTATTTGCAGAGCATTTTTTAAATTTATATGAGAAGACGAAGGATAAGTCATTTGATGAGGCCGCGTTAAAGCAGTGGCTAGAAAAAGGGGAGAGTAATACATGAGATTAGATaaa > 3:32599/1‑141 (MQ=255) agaaaaGAGTTATATATGAAAAAGTATTAGCAGAGCATTTTTTAAATTTATATGAGAAGACGAAGGATAAGTCATTTGAGGAGGCCGCGTTAAAAAAAAAAAAAAAAAAAGGGGAGAGTAATACATGAGATTAGATAAata < 2:215816/137‑1 (MQ=255) aGCCAAGAGTTATATTTGAAAAAGTATTTGCAGAGCATTTTTTATTTTTTTATGAGAAGACGAAGGATAAGTCATTTGATGAGGCCGCGTTAAAGCAGTGGCTAGAAAAAGGGGAGAGTAATACATGAGATTAGATAAata > 4:224903/1‑141 (MQ=255) aGCCAAGAGTTATATTTGAAAAAGTATTTGCAGAGCATTTTTTAAATTTATATGAGAAGACGAAGGATAAGTCATTTGATGAGGCCGCGTTAAAGCAGTGGCTAGAAAAAGGGGAGAGTAATACATGAGATTAGATAAata > 4:290756/1‑141 (MQ=255) ccAAGAGTTATATTTGNAAAAGTATTTGCNGAGCATTTTTTAAATTTATATGAGAAGACGAAGGATAAGTCATTTGATGAGGCCGCGTTAAAGCAGTGGCTAGAAAAAGGGGAGAGTAATACATGAGATTAGATAAATAtt > 1:750597/1‑141 (MQ=255) ccAAGAGTTATATTTGAAAAAGTATTTGCAGAGCATTTTTTAAATTTATATGAGAAGACGAAGGATAAGTCATTTGATGAGGCCGCGTTAAAGCAGTGGCTAGAAAAAGGGGAGAGTAATACATGAGATTAGATAAATAtt < 1:349934/141‑1 (MQ=255) agTTATATTTGAAAAAGTATTTGCAGAGCATTTTTTAAATTTATATGAGAAGACGAAGGATAAGTCATTTGATGAGGCCGCGTTAAAGCAGTGGCTAGAAAAAGGGGAGAGTAATACATGAGATTAGATAAATATTTaaaa > 3:292122/1‑141 (MQ=255) agTTATATTTGAAAAAGTATTTGCAGAGCATTTTTTAAATTTATATGAGAAGACGAAGGATAAGTCATTTGATGAGGCCGCGTTAAAGCAGTGGCTAGAAAAAGGGGAGAGTAATACATGAGATTAGATAAATATTTaaaa < 3:17315/141‑1 (MQ=255) gTTATATTTGAAAAAGTATTTGCAGAGCATTTTTTAAATTTATATGAGAAGACGAAGGATAAGTCATTTGATGAGGCCGCGTTAAAGCAGTGGCTAGAAAAAGGGGAGAGTAATACATGAGATTAGATAAATATTTAAAAg < 1:168801/141‑1 (MQ=255) atatTTGAAAAAGTATTTGCAGAGCATTTTTTTTTTTTATATTTTTTTTTTTATGATAAGTCATTTGATGAGGCCGCGTTAAAGCAGTGGCTAGAAAAAGGGGAGAGTAATACATGAGATTAGATAAATATTTAAAAGTAt > 3:384564/1‑141 (MQ=255) tatTTGAAAAAGTATTTGCAGAGCATTTTTTAAATTTATATGAGAAGACGAAGGATAAGTCATTTGATGAGGCCGCGTTAAAGCAGTGGCTAGAAAAAGGGGAGAGTAATACATGAGATTAGATAAATATTTAAAAGTATc > 3:415981/1‑141 (MQ=255) tatTTGAAAAAGTATTTGCAGAGCATTTTTTAAATTTATATGAGAAGACGAAGGATAAGTCATTTGATGAGGCCGCGTTAAAGCAGTGGCTAGAAAAAGGGGAGAGTAATACATGAGATTAGATAAATATTTAAAAGTATc > 3:48139/1‑141 (MQ=255) tttGAAAAAGTATTTGCAGAGCATTTTTTAAATTTATATGAGAAGACGAAGGATAAGTCATTTGATGAGGCCGCGTTAAAGCAGTGGCTAGAAAAAGGGGAGAGTAATACATGAGATTAGATAAATATTTAAAAGTATCAc < 1:492917/141‑1 (MQ=255) tttGAAAAAGTATTTGCAGAGCATTTTTTAAATTTATATGAGAAGACGAAGGATAAGTCATTTGATGAGGCCGCGTTAAAGCAGTGGCTAGAAAAAGGGGAGAGTAATACATGAGATTAGATAAATATTTAAAAGTATCAc < 2:998860/141‑1 (MQ=255) tGAAAAAGTATTTGCAGAGCATTTTTTAAATTTATATGAGAAGACGAAGGATAAGTCATTTGATGAGGCCGCGTTAAAGCAGTGGCTAGAAAAAGGGGAGAGTAATACATGAGATTAGATAAATATTTAAAAGTATCACgg > 4:338810/1‑141 (MQ=255) gAAAAAGTATTTGCAGAGCATTTTTTAAATTTATATGAGAAGACGAAGGATAAGTCATTTGATGAGGCCGCGTTAAAGCAGTGGCTAGAAAAAGGGGAGAGTAATACATGAGATTAGATAAATATTTAAAAGTATCACGGt < 1:794725/141‑1 (MQ=255) gTATTTGCAGAGCATTTTTTAAATTTATATGAGAAGACGAAGGATAAGTCATTTGATGAGGCCGCGTTAAAGCAGTGGCTAGAAAAAGGGGAGAGTAATACATGAGATTAGATAAATATTTAAAAGTATCACgg > 1:1044748/1‑134 (MQ=255) gTATTTGCAGAGCATTTTTTAAATTTATATGAGAAGACGAAGGATAAGTCATTTGATGAGGCCGCGTTAAAGCAGTGGCTAGAAAAAGGGGAGAGTAATACATGAGATTAGATAAATATTTAAAAGTATCACgg < 2:1044748/134‑1 (MQ=255) tATTTGCAGAGCATTTTTTAAATTTATATGAGAAGACGAAGGATAAGTCATTTGATGAGGCCGCGTTAAAGCAGTGGCTAGAAAAAGGGGAGAGTAATACATGAGATTAGATAAATATTTAAAAGTATCACGGTTAATaaa > 1:709823/1‑141 (MQ=255) aTTTGCAGAGCATTTTTTAAATTTATATGAGAAGACGAAGGATAAGTCATTTGATGAGGCCGCGTTAAAGCAGTGGCTAGAAAAAGGGGAGAGTAATACATGAGATTAGATAAATATTTAAAAGTATCACGGTTAATAAAg > 4:200675/1‑141 (MQ=255) tttGCAGAGCATTTTTTTAATGGATATGAGAAGACGAAGGATAAGTCATTTGATGAGGCCGCGTTAAAGCAGTGGCTAGAAAAAGGGGAGAGTAATACATGAGATTAGATAAATATTTAAAAGTATCACGGTTAATAAAGc < 1:332931/141‑1 (MQ=255) tttGCAGAGCATTTTTTAAATTTATATGAGAAGACGAAGGATAAGTCATTTGATGAGGCCGCGTTAAAGCAGTGGCTAGAAAAAGGGGAGAGTAATACATGAGATTAGATAAATATTTAAAAGTATCACGGTTAATAAAGc < 3:358741/141‑1 (MQ=255) tttGCAGAGCATTTTTTAAATTTATATGAGAAGACGAAGGATAAGTCATTTGATGAGGCCGCGTTAAAGCAGTGGCTAGAAAAAGGGGAGAGTAATACATGAGATTAGATAAATATTTAAAAGTATCACGGTTAATAAAGc < 3:358620/141‑1 (MQ=255) gCAGAGCATTTTTTAAATTTATATGAGAAGACGAAGGATAAGTCATTTGATGAGGCCGCGTTAAAGCAGTGGCTAGAAAAAGGGGAGAGTAATACATGAGATTAGATAAATATTTAAAAGTATCACGGTTAATAAAGCGAc < 2:525209/141‑1 (MQ=255) gCATTTTTTAAATTTATATGAGAAGACGAAGGATAAGTCATTTGATGAGGCCGCGTTAAAGCAGTGGCTAGAAAAAGGGGAGAGTAATACATGAGATTAGATAAATATTTAAAAGTATCACGGTTAATAAAGCGACGTACg < 1:645945/141‑1 (MQ=255) aTTTTTTAAATTTATATGAGAAGACGAAGGATAAGTCATTTGATGAGGCCGCGTTAAAGCAGTGGCTAGAAAAAGGGGAGAGTAATACATGAGATTAGATAAATATTTAAAAGTATCACGGTTAATAAAGCGACGTACGCt < 1:589143/141‑1 (MQ=255) tttAAATTTATATGAGAAGACGAAGGATAAGTCATTTGATGAGGCCGCGTTAAAGCAGTGGCTAGAAAAAGGGGAGAGTAATACATGAGATTAGATAAATATTTAAAAGTATCACGGTTAATAAAGCGACGTACGCTAGCa > 3:101786/1‑141 (MQ=255) ttAAATTTATATGAGAAGACGAAGGATAAGTCATTTGATGAGGCCGCGTTAAAGCAGTGGCTAGAAAAAGGGGAGAGTAATACATGAGATTAGATAAATATTTAAAAGTATCACGGTTAATAAAGCGACGTACGCTAGCaa < 1:559512/141‑1 (MQ=255) ttAAATTTATATGAGAAGACGAAGGATAAGTCATTTGATGAGGCCGCGTTAAAGCAGTGGCTAGAAAAAGGGGAGAGTAATACATGAGATTAGATAAATATTTAAAAGTATCACGGTTAATAAAGCGACGTACGCTAGCaa < 3:312841/141‑1 (MQ=255) tAAATTTATATGAGAAGACGAAGGATAAGTCATTTGATGAGGCCGCGTTAAAGCAGTGGCTAGAAAAAGGGGAGAGTAATACGTGAGATTAGATAAATATTTAAAAGTATCACGGTTAATAAAGCGACGTACGCTAGCaaa > 1:493779/1‑141 (MQ=255) aaaTTTATATGAGAAGACGAAGGATAAGTCATTTGATGAGGCCGCGTTAAAGCAGTGGCTAGAAAAAGGGGAGAGTAATACATGAGATTAGATAAATATTTAAAAGTATCACGGTTAATAAAGCGACGTACGCTAGCaaaa < 4:372126/141‑1 (MQ=255) | ATTCGTAGACACCCACACATATTTGGTGATGCCAATGCTGAAACTATCGATGACTTAAAAGAAATTTGGTCTAAGGCGAAAGATGCTGAAGGTAAACAGCCAAGAGTTATATTTGAAAAAGTATTTGCAGAGCATTTTTTAAATTTATATGAGAAGACGAAGGATAAGTCATTTGATGAGGCCGCGTTAAAGCAGTGGCTAGAAAAAGGGGAGAGTAATACATGAGATTAGATAAATATTTAAAAGTATCACGGTTAATAAAGCGACGTACGCTAGCAAAA > USA300TCH1516_ALE/543681‑543961 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |