Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 614,172 T→C 9.2% S828S (AGT→AGC sdrC → Serine‑aspartate repeat‑containing protein C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE614,1720TC 9.2% 336.7 / 3.3 142S828S (AGT→AGCsdrCSerine‑aspartate repeat‑containing protein C
Reads supporting (aligned to +/- strand):  ref base T (70/52);  new base C (4/12);  total (76/66)
Fisher's exact test for biased strand distribution p-value = 1.74e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 146 total aligned reads displayed.

TCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGA  >  USA300TCH1516_ALE/614032‑614312
                                                                                                                                            |                                                                                                                                            
 cGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTg                                                                                                                                             <  1:737040/141‑1 (MQ=255)
 cGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCAGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACACAGACAGCGACTCAGACTCAGACAGTg                                                                                                                                             >  1:458608/1‑141 (MQ=255)
     tAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTgattc                                                                                                                                         >  1:208480/1‑141 (MQ=255)
        cGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACCCACACAGTgattcaga                                                                                                                                      >  1:875736/1‑141 (MQ=255)
         gactcagactcaGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCGGACAGCGACTCAGACTCAGACAGTgattcagat                                                                                                                                     <  2:494147/141‑1 (MQ=255)
           ctcagactcagaTAGCGATTCAGATTCAGATTGCGATTCGGACTCAGACAGAGACTCAGATTCAGACTCAGATAGCGACTCAGACTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTgattcagattc                                                                                                                                   <  2:226333/141‑1 (MQ=255)
            tcagactcagaTAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGATTCAGACTCAGATACCGACTCAGATTGTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGCgattcagataca                                                                                                                                  >  2:387971/1‑141 (MQ=18)
                   cagaTAGCGATTCAGATTCAGATAGCGATTCAGACTCAGACAGTGATTCAGATTCAGACTCAGATAGAGATTCAGATTCTGACACCGATTCAGCCTCAGACTGCGAGTCAGACTCAGAACCCCATTCAGATACAGACaatg                                                                                                                           >  2:1045925/1‑138 (MQ=18)
                     gaTAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGAc                                                                                                                         >  4:31360/1‑141 (MQ=255)
                      aTACCGATTCAGATAAAGATTCCGATTCCGACAAAGACTCTGATTCAGATGCAGATTCAGATAGCGACTCAGACTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACt                                                                                                                        <  2:818825/141‑1 (MQ=17)
                           gattcagattcaGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACtcagat                                                                                                                   >  1:404393/1‑141 (MQ=255)
                           gattcagattcaGATAGAGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGCCTCAGATTCTGACAGCGATTCCGACTCAGACAGCGACTCTGACTCAGACAGTGATTCAGATTCAGACAGCGACtcagat                                                                                                                   <  1:648012/141‑1 (MQ=255)
                            attcagattcagATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACtcagatt                                                                                                                  <  3:197111/141‑1 (MQ=255)
                               cagattcagatAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACtcagattcag                                                                                                               >  2:468839/1‑141 (MQ=255)
                                cgattcagatAGCGATTCGGACTGAGCCGGCGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACtcagattcaga                                                                                                              <  1:1048816/140‑1 (MQ=255)
                                 gattcagatAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACtcagattcagat                                                                                                             <  3:405169/141‑1 (MQ=255)
                                     cagatAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcg                                                                                                         >  2:608047/1‑141 (MQ=255)
                                       gatAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgac                                                                                                       >  1:509480/1‑141 (MQ=255)
                                       gatAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgac                                                                                                       <  3:304014/141‑1 (MQ=255)
                                        ataacGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgact                                                                                                      >  2:391771/5‑141 (MQ=255)
                                           gCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgactcag                                                                                                   >  2:471893/1‑141 (MQ=255)
                                           gCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATGCAGACTCAGACAGCGACTCAGGCTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgactcag                                                                                                   >  1:919447/1‑141 (MQ=255)
                                            cGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgactcaga                                                                                                  >  4:32141/1‑141 (MQ=255)
                                            cGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgactcaga                                                                                                  <  4:355799/141‑1 (MQ=255)
                                             gATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgactcagac                                                                                                 >  2:364597/1‑141 (MQ=255)
                                                   gACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgactcagactcagat                                                                                           >  1:315195/1‑141 (MQ=255)
                                                     cTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgactcagactcagatag                                                                                         >  1:153754/1‑141 (MQ=255)
                                                     cTCAGACAGGGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGCGATTCAGATGCAGACAGCGACTCAGATacagaaagcgactcagactcagataa                                                                                         >  4:412985/1‑140 (MQ=18)
                                                      tCAGACTCTGATTAAGACTCAGACTCAGATAGAGACTCAGACTCTGACGGAGATTCAGACTCAGACAGCGACTCAGACTCTGACAGTGATTCAGATTCAGATAGCGACTCAGATtcagatagcgactcagactcagatagc                                                                                        <  2:97803/141‑1 (MQ=14)
                                                      tCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACTGCGATTCAGACTCAGACAGCGACTCAGACGCAGACAGTGACTCAGATTCAGACAGCGACTCAGATtcagatagcgactcagactcagatagc                                                                                        <  2:960552/141‑1 (MQ=255)
                                                           cAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgactcagactcagatagcgactc                                                                                   >  4:336581/1‑141 (MQ=255)
                                                             gTGATTCAGATTCAGACTCAGATAGCGACTCCGATACTAACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgactcagactcagatagcgactcag                                                                                 >  1:29901/1‑141 (MQ=255)
                                                              tGATTCAGATTCCGACTCAGATACCGACTCCGACTCAGACAGCGATTCCGACTCAGACAGCGACTCAGACTCAGACAGCGATTCAGATTCAGACAGCGACTCAGATtcagatagcgactcagactcagatagcgactcaga                                                                                <  2:765087/141‑1 (MQ=14)
                                                               gactcagattcagaCTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgactcagactcagatagcgactcagac                                                                               >  2:1025252/4‑141 (MQ=255)
                                                                  tcagattcagaCTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgactcagactcagatggcgactcagactca                                                                            <  1:579353/141‑1 (MQ=255)
                                                                   cagattcagaCTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGACTCAGACAGCGACtcaga                                                                                                                    <  3:124383/100‑1 (MQ=255)
                                                                   cagattcagaCTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGACTCAGACAGCGACtcaga                                                                                                                    >  4:124383/1‑100 (MQ=255)
                                                                     gattcagattcaGATAGCGATTCAGATTCCGACAGTGATTCCGACTCAGACAGCGATTCTGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgactcagactcagatagcgacccagactcagat                                                                         <  2:426600/141‑1 (MQ=11)
                                                                     gattcagaCTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgactcagactcagatagcgactcagactcagat                                                                         >  1:634104/1‑141 (MQ=255)
                                                                     gattcagaCTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgactcagactcagatagcgactcagactcagat                                                                         >  2:675990/1‑141 (MQ=255)
                                                                         cagaCTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgactcagactcagatagcgactcagactcagatagcg                                                                     >  3:137604/1‑141 (MQ=255)
                                                                             cTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgactcagactcagatagcgactcagactcagatagcgactc                                                                 <  1:736220/141‑1 (MQ=255)
                                                                             cTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGCGATTCAGATTCAGACAGCGACTCAGATtcagatagcgactcagactcagatagcgactcagactcagatagcgactc                                                                 >  4:384461/1‑141 (MQ=255)
                                                                               cAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgactcagactcagatagcgactcagactcagatagcgactcag                                                               <  3:177217/141‑1 (MQ=255)
                                                                               cAGATACCGACTCAGATTCTGACACCGATTCGGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGATTCAGACAGCGACTCAGAGtcagatagcgactcagactcagatagcgactcagactcagatagcgactcag                                                               <  2:57802/141‑1 (MQ=14)
                                                                                cgATAGCGACTCAGATTCAGACACAGATTCAGACTCAGACAGCGACTCAGACTCAGACACTGATTCAGATTCAGACAGCGACTCAGACtcagatagcgactcagactcagatagcgactcagactcagatagcaactcaga                                                              <  4:228958/140‑1 (MQ=14)
                                                                                 gATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGACAGCGATTCAGACTCAGATAGTGATTCAGATTCAGATAGTGATTCAGATtcagatagtgattcagattcagacagcgactcagactcagatagtgactcagac                                                             >  1:3243/1‑141 (MQ=1)
                                                                                   gagagACTCAGATTCAGACACCGATTCCGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCagatagcgactcagactcagatagcgactcagactcagatagcgactcagactc                                                           <  2:806434/137‑1 (MQ=11)
                                                                                    aGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCg                                                          >  2:674314/1‑141 (MQ=255)
                                                                                      cGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGa                                                        >  2:999990/1‑141 (MQ=255)
                                                                                      cGACTCAGACTCCGACAGCCACTCAGACTCAGACAGCGCCTCAGCCTCAGACAGCGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGa                                                        <  2:306758/141‑1 (MQ=14)
                                                                                       gACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGAt                                                       >  1:935827/1‑141 (MQ=255)
                                                                                        aCTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATa                                                      <  1:299020/141‑1 (MQ=255)
                                                                                        aCTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATa                                                      <  1:181920/141‑1 (MQ=255)
                                                                                           cAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCg                                                   <  1:778332/141‑1 (MQ=255)
                                                                                            aGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCga                                                  <  1:679066/141‑1 (MQ=255)
                                                                                             gATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCgat                                                 <  1:1086395/141‑1 (MQ=255)
                                                                                              aTTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCgatt                                                >  4:47506/1‑141 (MQ=255)
                                                                                               ttCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCgattc                                               >  4:189793/1‑141 (MQ=255)
                                                                                                  tGACAGCGATTCAGATTCAGACAGCGACTGAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCgattcaga                                            <  2:245543/141‑1 (MQ=255)
                                                                                                   gACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCgattcagat                                           <  1:13486/141‑1 (MQ=255)
                                                                                                   gACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCgattcacta                                           >  4:346238/1‑138 (MQ=255)
                                                                                                   gACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCgattcacat                                           >  2:708092/1‑141 (MQ=255)
                                                                                                   gACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGACAGCgattcagat                                           >  2:128868/1‑141 (MQ=255)
                                                                                                    aCAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCgattcagatt                                          >  1:152751/1‑141 (MQ=255)
                                                                                                     cAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCgattcagattc                                         >  3:395321/1‑141 (MQ=255)
                                                                                                     cAGCGAGTCAGACTCAGACACCGACTCAGGCCCCGACAGTGATTCAGACTCAGACAGCGCCTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCgattcagattc                                         <  2:770033/141‑1 (MQ=17)
                                                                                                       gCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGattcagattcag                                       >  2:113776/1‑141 (MQ=255)
                                                                                                           ttCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAg                                   <  1:629364/141‑1 (MQ=255)
                                                                                                           ttCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAg                                   <  3:151021/141‑1 (MQ=255)
                                                                                                           ctcagactcagccTCCGACTCAGACTCGGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAg                                   <  2:305175/140‑1 (MQ=14)
                                                                                                           ctcagactcagaaAGCGACTCAGCCCCCGCCTGCGAGCCAGACTCAGACAGCGCCTCAGAGTCAGATAGCGCCTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAg                                   <  2:980228/140‑1 (MQ=14)
                                                                                                             cagactcagacAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGAATCAGATGCAAACAGCg                                 >  2:939123/1‑141 (MQ=255)
                                                                                                               gactcagacAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGAc                               >  2:618174/1‑141 (MQ=255)
                                                                                                               gactcagacAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGAc                               >  1:838358/1‑141 (MQ=255)
                                                                                                               gactcagacAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGAc                               <  1:999883/141‑1 (MQ=255)
                                                                                                                 ctcagacAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACtc                             <  1:81181/141‑1 (MQ=255)
                                                                                                                   cagacAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACtcag                           >  3:106626/1‑141 (MQ=255)
                                                                                                                    agacAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACtcaga                          <  3:226539/141‑1 (MQ=255)
                                                                                                                    agacAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACtcaga                          <  1:230838/141‑1 (MQ=255)
                                                                                                                     gacAGCGACTCAGACTCAGACAGTGATTAAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACtcagat                         >  1:574978/1‑141 (MQ=255)
                                                                                                                        aGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACtcagattca                      >  3:213019/1‑141 (MQ=255)
                                                                                                                         gCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACtcagattcag                     >  3:332615/1‑141 (MQ=255)
                                                                                                                         gCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACtcagatacag                     >  2:506223/1‑141 (MQ=255)
                                                                                                                          cGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACtcagattcaga                    <  1:685980/141‑1 (MQ=255)
                                                                                                                          cGACTCAGACTCAGACACAGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACtcagattcaga                    <  2:1047638/141‑1 (MQ=255)
                                                                                                                           gactcagactcaGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACtcagattcagat                   <  1:768007/141‑1 (MQ=255)
                                                                                                                            actcagactcagACAGTGAGTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCAGATa                  <  1:1046664/141‑1 (MQ=255)
                                                                                                                               cagactaagacAGTGATTCAGATTCAGACAGCGACTCAGATGCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGAATAGCGATCCACATTCACACAGCGACTCAAATGCAGACAGCg               >  4:22246/1‑141 (MQ=17)
                                                                                                                                cgactcagacAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGa              <  2:459618/140‑1 (MQ=255)
                                                                                                                                 gactcagacAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGAt             >  1:1013150/1‑141 (MQ=255)
                                                                                                                                 gactcagacAGCGACTCAGATTCAGATAGCGACTCAGATTCAGATAGCGACGCAGACTCAGATAGCGACTCAGACTCTGATAGCGACTCAGACTCGGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGATAGCGAt             <  4:229401/141‑1 (MQ=14)
                                                                                                                                   ttcagacAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGATTc           <  1:520922/140‑1 (MQ=255)
                                                                                                                                   ctcagacAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGATTc           <  1:273426/141‑1 (MQ=255)
                                                                                                                                   ctcagacAGCGATTCAGGTTCAGACAGCGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGATTc           <  4:205250/141‑1 (MQ=255)
                                                                                                                                    tcagacAGTGATTCAGATTCATACAGCGTCTCAGATCCAGATAGCGATTCACCACCAGATAGAAAATCAGACTCAGAGAGCGTCCCAGACTCAGGTAGCGATTCAGATTCAGTCAGCGACTCAGATTCAGCTAGCGATTCg          <  4:198990/141‑1 (MQ=2)
                                                                                                                                      agacAGAGGTTCAGACTCCGACAGCGACTCAGACTCAGACGGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGATTCGGa        <  2:534390/141‑1 (MQ=14)
                                                                                                                                         cAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTc     <  2:105116/141‑1 (MQ=255)
                                                                                                                                           gTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTAAGATAGCGATTCGGACTCAg   >  1:824961/1‑141 (MQ=255)
                                                                                                                                            tGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGa  <  1:15001/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                            
TCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGA  >  USA300TCH1516_ALE/614032‑614312

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: